CN108633735B - Method for breeding new variety of dendrobium - Google Patents
Method for breeding new variety of dendrobium Download PDFInfo
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- CN108633735B CN108633735B CN201810411077.6A CN201810411077A CN108633735B CN 108633735 B CN108633735 B CN 108633735B CN 201810411077 A CN201810411077 A CN 201810411077A CN 108633735 B CN108633735 B CN 108633735B
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- culture medium
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- new variety
- dendrobium
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a dendrobium new variety breeding method, which comprises the steps of mutagenesis culture, proliferation culture, rooting and strong seedling culture and seedling hardening, and the dendrobium new variety seedling is obtained. The invention takes the lateral bud and the terminal bud of the strain with excellent flower shape and flower color as the explant, the explant is induced by the induced mutation culture medium, the excellent individual plant with mutation is selected from the explant, and the excellent individual plant is expanded to form a new variety, the culture period is short, and the survival rate is high.
Description
Technical Field
The invention relates to a breeding method of a new tropical orchid variety, in particular to a breeding method of a new dendrobium variety.
Background
The dendrobium belongs to tropical orchid and has high ornamental value and medicinal value. Due to the extremely high economic value of dendrobium, wild resources are dug in a transitional way. Most of dendrobium varieties popular in the market are hybrid varieties. The existing dendrobium crossbreeding period is long, and a new crossbreed can be cultivated within 7-8 years.
Disclosure of Invention
The invention aims to provide a dendrobium new variety breeding method, which solves the problem of long culture period in the prior art.
A method for breeding a new variety of dendrobium comprises the following steps:
selecting side buds and terminal buds of strains with excellent flower shapes and colors as explants, and carrying out mutagenesis culture in a mutagenesis culture medium for about 40 days to form protocorm-like bodies; the formula of the mutagenesis culture medium is as follows: 5g of sucrose, 10g of peptone, 2.5g of agar powder, 1.5g of ethyl methanesulfonate, 0.2g of clomazone and water to a constant volume of 1L;
transferring the protocorm-like body to an enrichment culture medium for proliferation differentiation culture to form a seedling; the formula of the proliferation culture medium is as follows: 1g of Huabao 1, 1g of Huabao 2, 5g of peptone, 2g of coconut juice, 20g of cane sugar, 20g of agar powder and water to a constant volume of 1L;
and step three, transferring the small seedlings into a strong seedling rooting culture medium for strong seedling rooting, wherein the strong seedling rooting culture medium is 1/2MS +0.5mg/L NAA, the rooting culture is about 30 days, transferring the medium into a pergola for hardening the seedlings for 7-15 days, then transplanting the medium into water moss for planting, transplanting the seedlings into a pot when the seedlings grow to about 15cm, transplanting substrates are coconut pieces and vesuvianite, the volume ratio of the coconut pieces to the vesuvianite is 1:1, when the plants bloom, selecting the plants with variation and good ornamental performance, and performing propagation to form new varieties.
Preferably, in the first step and the second step, the culture temperature is 20-25 ℃, the illumination intensity is 1500-.
The invention has the following beneficial effects:
the new dendrobium variety breeding method provided by the invention takes the lateral buds and terminal buds of strains with excellent flower shapes and colors as explants, induces the strains through the induced mutation culture medium, breeds the excellent single plants with variation from the induced mutation culture medium, and forms a new variety through propagation, the culture period is short, and the survival rate is high.
Detailed Description
The following examples are provided to further illustrate the embodiments of the present invention.
Examples
A method for breeding a new variety of dendrobium comprises the following steps:
selecting side buds and terminal buds of dendrobium nobile lindl with excellent flower shape and flower color as explants, and culturing the side buds and the terminal buds in a mutagenesis culture medium for 40 days to form protocorm-like bodies; the formula of the mutagenesis culture medium is as follows: 5g of sucrose, 10g of peptone, 2.5g of agar powder, 1.5g of ethyl methanesulfonate, 0.2g of clomazone and water to a constant volume of 1L; the culture temperature is 20-25 ℃, the illumination intensity is 1500-;
continuing to develop the protocorm-like body, developing partial protocorm-like body into a small plant, transferring the protocorm-like body forming the small plant into a proliferation culture medium, and performing proliferation differentiation culture for about 30 days to form a small seedling; the formula of the proliferation culture medium is as follows: 1g of Huabao 1, 1g of Huabao 2, 5g of peptone, 2g of coconut juice, 20g of cane sugar, 20g of agar powder and water to a constant volume of 1L; the culture temperature is 20-25 ℃, the illumination intensity is 1500-;
transferring the plantlets into a strong seedling rooting culture medium for strong seedling rooting, wherein the strong seedling rooting culture medium is 1/2MS +0.5mg/L NAA, when the rooting and strong seedling culture is carried out for 30 days, the plantlets grow to 3-4 cm and have 2-3 roots, transferring the plantlets into a pergola for hardening the plantlets for 10 days, and then transplanting the plantlets into water moss for planting; the seedlings grow to about 15cm, the seedlings are transplanted into pots, transplanting substrates are coconut pieces and vesuvianite, and the volume ratio of the coconut pieces to the vesuvianite is 1: 1; when the plants bloom, the plants with variation and good ornamental value are selected and expanded to form new varieties.
The composition and content of the MS medium are shown in Table 1.
TABLE 1 MS Medium composition
| Composition (I) | Concentration (mg/L) |
| Potassium nitrate KNO3 | 1900 |
| Ammonium nitrate NH4NO3 | 1650 |
| Potassium dihydrogen phosphate KH2PO4 | 170 |
| Magnesium sulfate MgSO4·7H2O | 370 |
| Calcium chloride CaCl2·2H2O | 440 |
| Potassium iodide KI | 0.83 |
| Boric acid H3BO3 | 6.2 |
| Manganese sulfate MnSO4·4H2O | 22.3 |
| Zinc sulfate ZnSO4·7H2O | 8.6 |
| Sodium molybdate Na2MoO4·2H2O | 0.25 |
| Copper sulfate CuSO4·5 H2O | 0.025 |
| Cobalt chloride CoCl2·6H2O | 0.025 |
| Disodium ethylenediaminetetraacetate Na2.EDTA | 37.25 |
| Ferrous sulfate FeSO4·7H2O | 27.85 |
| Glycine | 2 |
| Thiamine hydrochloride VB1 | 0.1 |
| Pyridoxine hydrochloride VB6 | 0.5 |
| Sucrose | 30g/L |
| Agar | 7g/L |
In the embodiment, the dendrobium variety with excellent characters is selected, the lateral buds and the terminal buds of the strain with excellent flower shape and flower color are used as explants, after about 300-fold and 310-day culture, part of the plants flower, the flower shape is novel, the flower color is various and odditious, and the survival rate after transplantation reaches more than 90%.
The method for breeding the new dendrobium variety provided by the invention takes the stem node and the stem tip of a strain with excellent flower shape and flower color as explants, performs mutagenesis culture on the explant to form a new variety, and performs culture such as rooting and seedling strengthening to grow the new variety into a plant, so that the culture period is short and the survival rate is high.
Claims (1)
1. A dendrobium new variety breeding method is characterized in that: the method comprises the following steps:
selecting side buds and terminal buds of strains with excellent flower shapes and colors as explants, and carrying out mutagenesis culture in a mutagenesis culture medium for about 40 days to form protocorm-like bodies; the formula of the mutagenesis culture medium is as follows: 5g of sucrose, 10g of peptone, 2.5g of agar powder, 1.5g of ethyl methanesulfonate, 0.2g of clomazone and water to a constant volume of 1L;
transferring the protocorm-like body to a proliferation culture medium for proliferation and differentiation culture to form a small seedling; the formula of the proliferation culture medium is as follows: 1g of Huabao 1, 1g of Huabao 2, 5g of peptone, 2g of coconut juice, 20g of cane sugar, 20g of agar powder and water to a constant volume of 1L;
transferring the plantlets into a strong seedling rooting culture medium for strong seedling rooting, wherein the strong seedling rooting culture medium is 1/2MS +0.5mg/L NAA, the rooting and strong seedling culture medium is cultured for about 30 days, transferring the plantlets into a shady shed for hardening seedlings for 7-15 days, and then transplanting the plantlets into water moss for planting; transplanting the seedlings into a pot when the seedlings grow to about 15cm, wherein transplanting substrates are coconut pieces and vesuvianite, and the volume ratio of the coconut pieces to the vesuvianite is 1: 1; when the plants bloom, selecting the plants which have variation and good ornamental value, and carrying out propagation to form a new variety;
in the first step and the second step, the culture temperature is 20-25 ℃, the illumination intensity is 1500-1800lx, and the illumination time is 10-12 h/day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201810411077.6A CN108633735B (en) | 2018-05-02 | 2018-05-02 | Method for breeding new variety of dendrobium |
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| CN201810411077.6A CN108633735B (en) | 2018-05-02 | 2018-05-02 | Method for breeding new variety of dendrobium |
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| CN108633735A CN108633735A (en) | 2018-10-12 |
| CN108633735B true CN108633735B (en) | 2021-12-17 |
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| CN201810411077.6A Expired - Fee Related CN108633735B (en) | 2018-05-02 | 2018-05-02 | Method for breeding new variety of dendrobium |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110250005B (en) * | 2019-07-22 | 2023-04-07 | 中国热带农业科学院热带作物品种资源研究所 | Cultivation method of new cymbidium goeringii variety |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103314861A (en) * | 2013-07-08 | 2013-09-25 | 中国科学院华南植物园 | Crossbreeding method in dendrobium test tube |
| CN104012401A (en) * | 2013-02-28 | 2014-09-03 | 江南大学 | Rapid propagation method of dendrobium huoshanense |
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- 2018-05-02 CN CN201810411077.6A patent/CN108633735B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012401A (en) * | 2013-02-28 | 2014-09-03 | 江南大学 | Rapid propagation method of dendrobium huoshanense |
| CN103314861A (en) * | 2013-07-08 | 2013-09-25 | 中国科学院华南植物园 | Crossbreeding method in dendrobium test tube |
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| CN108633735A (en) | 2018-10-12 |
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