CN108627252A - Sequenator image taking temperature alarming method and system - Google Patents
Sequenator image taking temperature alarming method and system Download PDFInfo
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- CN108627252A CN108627252A CN201710178501.2A CN201710178501A CN108627252A CN 108627252 A CN108627252 A CN 108627252A CN 201710178501 A CN201710178501 A CN 201710178501A CN 108627252 A CN108627252 A CN 108627252A
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- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000008859 change Effects 0.000 claims abstract description 11
- 238000003384 imaging method Methods 0.000 claims description 33
- 108090000623 proteins and genes Proteins 0.000 claims description 19
- 238000012163 sequencing technique Methods 0.000 claims description 14
- 239000011324 bead Substances 0.000 claims description 6
- 239000000571 coke Substances 0.000 claims 1
- 230000009467 reduction Effects 0.000 description 9
- 238000010586 diagram Methods 0.000 description 4
- 230000006978 adaptation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 230000011218 segmentation Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J5/10—Radiation pyrometry, e.g. infrared or optical thermometry using electric radiation detectors
- G01J5/20—Radiation pyrometry, e.g. infrared or optical thermometry using electric radiation detectors using resistors, thermistors or semiconductors sensitive to radiation, e.g. photoconductive devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J5/00—Radiation pyrometry, e.g. infrared or optical thermometry
- G01J2005/0077—Imaging
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- Spectroscopy & Molecular Physics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a kind of sequenator image taking temperature alarming methods comprising is focused the same reference position of sample to obtain two pinpointed focus values twice in different moments；According to two pinpointed focus value computing environment temperature change values；And carry out alarm when the variation of ambient temperature value calculated is more than temperature difference threshold.The present invention also provides a kind of sequence instrument image taking temperature alarm system, sequenator image taking temperature alarming method and system of the invention can alarm to environment temperature extremely when using sequenator.
Description
Technical field
The present invention relates to gene sequencing fields, it is more particularly related to a kind of sequenator image taking temperature report
Alarm method and system.
Background technology
Gene sequencing field usually requires to carry out image taking and identification to sample, since the camera lens of imaging device observes model
The area much smaller than sample is enclosed, therefore camera lens every time can only shoot the part of sample.Prior art generally use is continuous
The mode of stepping shoots the multiple regions of the observation scope of adaptation imaging device successively, is shot successively to each zonule.
Several progress in the generally selected multiple regions of the prior art focus the corresponding focal length of acquisition automatically, and other regions pass through Linear Quasi
The mode of conjunction calculates pinpointed focus when shooting, and imaging device shoots zonule according to the pinpointed focus of calculating.By
High in the precision requirement of gene sequencing field image taking, room temperature variation leads to Linear Quasi to caused phenomenon of expanding with heat and contract with cold
The pinpointed focus that the mode of conjunction calculates will appear apparent error, and then lead to focal length occur when gene sequencing progress image taking
The case where distortion.
Invention content
The purpose of the present invention is to provide a kind of sequenator image taking temperature alarming method and systems, it is intended to solve environment
The technical issues of temperature alarming is carried out to sequenator when temperature anomaly.
A kind of sequenator image taking temperature alarming method includes：
The same reference position of sample is focused twice to obtain two pinpointed focus values in different moments；
According to two pinpointed focus value computing environment temperature change values；And
Alarm is carried out when the variation of ambient temperature value of calculating is more than temperature difference threshold.
Further, the sequenator image taking temperature alarming method further includes：In advance in difference at room temperature to sample
Same reference position carries out multi-focusing to obtain the temperature profile of pinpointed focus variation, the meter of the variation of ambient temperature value
The temperature profile changed by two pinpointed focus values and the pinpointed focus is calculated to obtain.
Further, the sequenator image taking temperature alarming method further includes the changing value of sense ambient temperature.
Further, when the sensing value of variation of ambient temperature is more than compensation threshold value, according to pinpointed focus variation
Focal length distortion when temperature profile compensation shooting sample.
Further, the sample is the high-throughput gene sequencing sample for including multiple magnetic beads.
A kind of sequenator image taking temperature alarm system includes：
Article carrying platform, the article carrying platform are used for fixed sample；
Imaging device, for carrying out image taking to fixed sample on article carrying platform, the imaging device is in different moments pair
The same reference position of sample is focused twice to obtain two pinpointed focus values；
Temperature computation module, for according to two pinpointed focus value computing environment temperature change values；And
Alarm module sends out alarm for working as variation of ambient temperature value more than temperature difference threshold.
Further, the sequenator image taking temperature alarm system further includes：Curve generation module, for controlling
Different imaging devices at room temperature carry out multi-focusing to obtain the temperature curve of pinpointed focus variation to the same reference position of sample
The calculating of figure, the variation of ambient temperature value is obtained by the temperature profile that two pinpointed focus values and the pinpointed focus change
.
Further, the sequenator image taking temperature alarm system further includes temperature sensing module, for sensing ring
The changing value of border temperature.
Further, the sequenator image taking temperature alarm system further includes focal length compensating module, for control at
As device variation of ambient temperature sensing value be more than compensation threshold value when, according to the pinpointed focus variation temperature profile mend
Repay focal length distortion when shooting sample.
Further, the sample is the high-throughput gene sequencing sample for including multiple magnetic beads.
Compared with the existing technology, sequenator image taking temperature alarming method and system of the invention, by focusing twice
Different focal length calculate room temperature variation, alarm is carried out when temperature anomaly, it is larger in temperature fluctuation that this avoid users
In the case of use sequenator.In addition, the present invention also it is different use at room temperature imaging device to the same reference position of sample into
Row multi-focusing can be according to environment when carrying out gene sequencing image taking with the temperature profile for obtaining pinpointed focus variation
Focal length when the temperature profile compensation shooting sample that temperature change is changed by pinpointed focus is distorted, and it is automatic right not only to accelerate
Burnt speed, and so that more accurate after the pinpointed focus compensation that linear fit mode calculates, the clarity of image taking is more
It is good.
Description of the drawings
Fig. 1 is the flow diagram of first embodiment of the invention sequenator image taking temperature alarming method.
Fig. 2 is the schematic diagram focused twice in first embodiment of the invention sequenator image taking temperature alarming method.
Fig. 3 is an example of the temperature profile of pinpointed focus provided by the invention variation.
Fig. 4 is the flow diagram of second embodiment of the invention sequenator image taking temperature alarming method.
Fig. 5 is another example of the temperature profile of pinpointed focus provided by the invention variation.
Fig. 6 is the structural schematic diagram of third embodiment of the invention sequenator image taking temperature alarm system.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, below in conjunction with drawings and the embodiments,
The present invention will be described in further detail.
- Fig. 3 is please referred to Fig.1, first embodiment of the invention provides a kind of sequenator image taking temperature alarming method,
Including step S10-S13.The sequenator image taking temperature alarming method of the present invention is applied to gene sequencer, and the gene is surveyed
Sequence instrument includes article carrying platform, imaging device and alarm module.For fixing and carrying sample, the sample is the article carrying platform
High-throughput gene sequencing sample including multiple magnetic beads.Article carrying platform described in the present embodiment is XY platforms, can be from reduction point
Start to move along X-coordinate axis direction and/or move along Y coordinate axis direction, to facilitate imaging device to being fixed on article carrying platform
Sample be focused and take pictures.In the present embodiment, the imaging device includes camera lens and obtains the ccd image sensor of image.
Step S11 is focused twice to obtain two pinpointed focus the same reference position of sample in different moments
Value.It, can be with prefixed time interval, such as 1-2 minutes, preferable embodiment party between the different moments in the present embodiment with reference to figure 2
Time interval described in formula is 1 minute.Together with reference to figure 2, in the present embodiment, the focusing range of imaging device is much smaller than sample 20
Area, the shooting of gene sequencing sample is carried out with the preferable rectangle shot region 30 of the imaging effect being pre-selected.It is practical
When shooting, the rectangle shot region 30 chosen in advance is divided into the zonule 31 of the focusing range of multiple adaptation imaging devices.
In present embodiment, multiple subregions 31 it is arranged in arrays and include the multirow subregion A1-A5 arranged along X-coordinate axis direction,
B1-B5, C1-C5 and D1-D5.In the present embodiment, the centre coordinate for pre-defining the subregion A1 nearest apart from reduction point o is ginseng
Examine position.Can also be selected in other embodiment the center of any one in subregion A1-A5, B1-B5, C1-C5 and D1-D5 for
Reference position.In alternate embodiment, also can not to sample 20 carry out region segmentation, in sample range optionally a little as reference
Position.In the present embodiment, XY platforms 10 are moved from reduction point o along Y direction, such as circuit Y1 in Fig. 2, make imaging device and first
The Y coordinate of row subregion A1-A5（0, Y1）Alignment.Then XY platforms 10 are moved along X-coordinate, such as circuit X1 in Fig. 2, i.e., from multiple
The X-coordinate in site（0, Y1）Start mobile XY platforms 10, imaging device is made to carry out the to being located at subregion A1 at the first moment
It is primary to focus, to obtain the first pinpointed focus.After preset time, such as 1 minute, moved again from reduction point o along Y direction
Dynamic XY platforms 10, pass through circuit Y1 and circuit X1 successively, and imaging device is made to carry out second to being located at subregion A1 at the second moment
Secondary focusing, to obtain the second pinpointed focus.In the present embodiment, the light conditions focused twice are identical.
Step S12, according to two pinpointed focus value computing environment temperature change values.In the present embodiment, can also further it wrap
Step S10 is included, for carrying out multi-focusing to the same reference position of sample at room temperature different to obtain pinpointed focus change in advance
The temperature profile of change, what the calculating of the variation of ambient temperature value was changed by two pinpointed focus values and the pinpointed focus
Temperature profile obtains.In one embodiment, if Fig. 3 is the temperature profile of pinpointed focus variation, the first pinpointed focus Z1 is
2280 microns, the second pinpointed focus Z2 is that 2250 microns, then the first pinpointed focus and the second pinpointed focus difference are 30 microns, is led to
Cross the temperature profile that pinpointed focus in Fig. 3 changes can calculate the first pinpointed focus Z1 and the second pinpointed focus Z2 it
Between corresponding variation of ambient temperature value be 5 degree.In alternate embodiments, the step S10 can be omitted, at this point, user can be with
The corresponding variation of ambient temperature value of mathematic interpolation of two pinpointed focus values is estimated according to use experience.
Step S13 carries out alarm when the variation of ambient temperature value of calculating is more than temperature difference threshold.In the present embodiment, if
Temperature difference threshold is 2 degree, then when the variation of ambient temperature value that step S12 is calculated is 5 degree, then alarm module carries out alarm,
For prompting user.The prompt can be word pop-up prompt such as " the current environmental temperature variation of display interface
It is larger, equipment performance may be influenced, please used later ", can also be audible alarm prompt.
Referring to FIG. 4, second embodiment of the invention provides a kind of sequenator image taking temperature alarming method comprising
Step S10-S15, second embodiment and first embodiment the difference is that, second embodiment further comprises walking
Rapid S14 and step S15.
Step S14, the changing value of sense ambient temperature.Highly sensitive temperature-sensitive sticker may be used in the present embodiment to gene
The environment temperature of sequenator, such as indoor temperature around sequenator carry out real-time detection and calculate prefixed time interval correspondence
Sense ambient temperature changing value.
Step S15, when the sensing value of variation of ambient temperature is more than compensation threshold value, according to the temperature of pinpointed focus variation
Focal length distortion when line chart of writing music compensation shooting sample.With reference to figure 5, in an embodiment, it is assumed that sample needs the part shot
Default focal length be 2284 microns, the changing value of sense ambient temperature is to reduce by 4 degree, then passes through pinpointed focus variation in Fig. 5
It is 24 microns that temperature profile, which can calculate the focal length variations, then the real focal length of the sample shooting after compensating should be
2284 microns subtract 24 microns, i.e., 2260 microns.The compensation threshold value described in the present embodiment is more than zero and is less than the temperature difference threshold
Value, preferably, the compensation threshold value can set the arbitrary value between 3-5 degree.
Compared with the existing technology, sequenator image taking temperature alarming method of the invention by different moments twice
The different focal length of focusing calculates room temperature variation, alarm is carried out when temperature anomaly, this avoid users in temperature fluctuation
Sequenator is used in the case of larger.In addition, the present invention also uses imaging device to the same reference bit of sample at room temperature different
Setting progress multi-focusing can basis when carrying out gene sequencing image taking with the temperature profile for obtaining pinpointed focus variation
Focal length when the temperature profile compensation shooting sample that variation of ambient temperature is changed by pinpointed focus is distorted, and is not only accelerated certainly
The speed of dynamic focusing, and make it is more accurate after pinpointed focus compensation that linear fit mode calculates, image taking it is clear
Degree is more preferable.
Referring to FIG. 6, third embodiment of the invention provides a kind of sequenator image taking temperature alarm system 100, institute
It includes article carrying platform 110, imaging device 120, temperature computation module 130, report to state sequenator image taking temperature alarm system 100
Alert module 140, curve generation module 150, temperature sensing module 160 and focal length compensating module 170.It is described in present embodiment
Module can execute the integrated circuit of specific function, can also be that storage executes completion spy in memory and by processor
Determine the software program of function.
For the article carrying platform 110 for fixing and carrying sample, the sample is the high-throughput gene for including multiple magnetic beads
Sample is sequenced.Article carrying platform described in the present embodiment be XY platforms, can since reduction point along X-coordinate axis direction move and/
Or moved along Y coordinate axis direction, it is taken pictures with facilitating imaging device to be focused the sample being fixed on article carrying platform.This implementation
In example, the imaging device includes camera lens and obtains the ccd image sensor of image.
The imaging device 120 is used to carry out image taking to fixed sample on article carrying platform, and the imaging device exists
Different moments focus twice to obtain two pinpointed focus values the same reference position of sample.In the present embodiment, it is described not
Can be with prefixed time interval, such as 1-2 minutes between in the same time, time interval described in better embodiment is 1 minute.Together
With reference to figure 2, in the present embodiment, the focusing range of imaging device is much smaller than the area of sample 20, the shooting to gene sequencing sample
It is carried out with the preferable rectangle shot region 30 of the imaging effect being pre-selected.When actual photographed, the rectangle chosen in advance is taken pictures
Region 30 is divided into the zonule 31 of the focusing range of multiple adaptation imaging devices.In present embodiment, multiple subregions 31 are in
Matrix arrangement and include multirow subregion A1-A5, B1-B5, C1-C5 and the D1-D5 arranged along X-coordinate axis direction.The present embodiment
In, the centre coordinate for pre-defining the subregion A1 nearest apart from reduction point o is reference position.It can also be selected in other embodiment
The center for selecting any one in subregion A1-A5, B1-B5, C1-C5 and D1-D5 is reference position.In alternate embodiment, also may be used
Region segmentation is not carried out to sample 20, optionally a little as with reference to position in sample range.In the present embodiment, from reduction point o
XY platforms 10 are moved along Y direction, such as circuit Y1 in Fig. 2, make the Y coordinate of imaging device and the first row subregion A1-A5（0,
Y1）Alignment.Then XY platforms 10 are moved along X-coordinate, such as circuit X1 in Fig. 2, i.e., from the X-coordinate of reduction point（0, Y1）Start to move
Dynamic XY platforms 10, make imaging device carry out first time focusing to being located at subregion A1 at the first moment, best to obtain first
Focal length.After preset time, such as 1 minute, XY platforms 10 are moved from reduction point o along Y direction again, pass through circuit successively
Y1 and circuit X1 makes imaging device carry out second of focusing to being located at subregion A1 at the second moment, best burnt to obtain second
Away from.In the present embodiment, the light conditions focused twice are identical.
The temperature computation module 130 is used for according to two pinpointed focus value computing environment temperature change values.The present embodiment
In, it can also further comprise curve generation module 150, be used in advance at room temperature carry out the same reference position of sample different
Multi-focusing is to obtain the temperature profile of pinpointed focus variation, in preferred embodiment, the calculating of the variation of ambient temperature value
The temperature profile changed by two pinpointed focus values and the pinpointed focus obtains.In one embodiment, if Fig. 3 is best burnt
Temperature profile away from variation, the first pinpointed focus Z1 are 2280 microns, and the second pinpointed focus Z2 is 2250 microns, then first most
Good focal length and the second pinpointed focus difference are 30 microns, and the temperature profile changed by pinpointed focus in Fig. 3 can calculate
Corresponding variation of ambient temperature value is 5 degree between the first pinpointed focus Z1 and the second pinpointed focus Z2.
The variation of ambient temperature value that the alarm module 140 is used to be calculated in temperature computation module 130 is more than temperature difference threshold
Value carries out alarm.In the present embodiment, if temperature difference threshold is 2 degree, then when variation of ambient temperature value is 5 degree, then mould of alarming
Block carries out alarm, and the alarm can be the word pop-up prompt of display interface, can also be audible alarm prompt.
The temperature sensing module 160 is used for the changing value of sense ambient temperature.Temperature sensing module 160 in the present embodiment
Environment temperature of the highly sensitive temperature-sensitive sticker to gene sequencer may be used, such as indoor temperature carries out real-time detection and calculates
Go out the changing value of the corresponding sense ambient temperature of prefixed time interval.
The focal length compensating module 170 is used for when the sensing value of variation of ambient temperature is more than compensation threshold value, according to described
Focal length distortion when the temperature profile compensation shooting sample of pinpointed focus variation.With reference to figure 5, in an embodiment, it is assumed that sample
The focal length for the part that this needs is shot is 2284 microns, and the changing value of sense ambient temperature is to reduce by 4 degree, then by Fig. 5 most
It is 24 microns that the temperature profile of good focal length variations, which can calculate the focal length variations, then the reality of the sample shooting after compensating
Focal length should be 2284 microns and subtract 24 microns, i.e., 2260 microns.
Compared with the existing technology, sequenator image taking temperature alarm system of the invention by different moments twice
The different focal length of focusing calculates room temperature variation, alarm is carried out when temperature anomaly, this avoid users in temperature fluctuation
Sequenator is used in the case of larger.In addition, the present invention also uses imaging device to the same reference bit of sample at room temperature different
Setting progress multi-focusing can basis when carrying out gene sequencing image taking with the temperature profile for obtaining pinpointed focus variation
Focal length when the temperature profile compensation shooting sample that variation of ambient temperature is changed by pinpointed focus is distorted, and is not only accelerated certainly
The speed of dynamic focusing, and make it is more accurate after pinpointed focus compensation that linear fit mode calculates, image taking it is clear
Degree is more preferable.
The foregoing is merely the better embodiments of the present invention, are not intended to limit the invention, all the present invention's
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within spirit and principle.
Claims (10)
1. a kind of sequenator image taking temperature alarming method, it is characterised in that including：
The same reference position of sample is focused twice to obtain two pinpointed focus values in different moments；
According to two pinpointed focus value computing environment temperature change values；And
Alarm is carried out when the variation of ambient temperature value of calculating is more than temperature difference threshold.
2. sequenator image taking temperature alarming method according to claim 1, it is characterised in that further comprise：
Multi-focusing is carried out to the same reference position of sample at room temperature to obtain the temperature song of pinpointed focus variation different in advance
The temperature profile that the calculating of line chart, the variation of ambient temperature value passes through two pinpointed focus values and the pinpointed focus changes
It obtains.
3. sequenator image taking temperature alarming method according to claim 2, which is characterized in that further comprise sensing
The changing value of environment temperature.
4. sequenator image taking temperature alarming method according to claim 3, it is characterised in that：Work as variation of ambient temperature
Sensing value be more than compensation threshold value when, according to the pinpointed focus variation temperature profile compensation shooting sample when focal length lose
Very.
5. sequenator image taking temperature alarming method according to claim 1, it is characterised in that：The sample is to include
The high-throughput gene sequencing sample of multiple magnetic beads.
6. a kind of sequenator image taking temperature alarm system, it is characterised in that including：
Article carrying platform, the article carrying platform are used for fixed sample；
Imaging device, for carrying out image taking to fixed sample on article carrying platform, the imaging device is in different moments pair
The same reference position of sample is focused twice to obtain two pinpointed focus values；
Temperature computation module, for according to two pinpointed focus value computing environment temperature change values；And
Alarm module sends out alarm for working as variation of ambient temperature value more than temperature difference threshold.
7. sequenator image taking temperature alarm system according to claim 6, it is characterised in that further comprise：
Curve generation module, for control different imaging devices at room temperature to the same reference position of sample carry out multi-focusing with
Obtain the temperature profile of pinpointed focus variation, the calculating of the variation of ambient temperature value passes through two pinpointed focus values and described
The temperature profile of pinpointed focus variation obtains.
8. sequenator image taking temperature alarm system according to claim 7, which is characterized in that further comprise temperature
Sensing module is used for the changing value of sense ambient temperature.
9. sequenator image taking temperature alarm system according to claim 8, it is characterised in that：Further comprise focal length
Compensating module, for controlling imaging device when the sensing value of variation of ambient temperature is more than compensation threshold value, according to the best coke
Temperature profile compensation away from variation shoots focal length distortion when sample.
10. sequenator image taking temperature alarm system according to claim 6, it is characterised in that：The sample is packet
Include the high-throughput gene sequencing sample of multiple magnetic beads.
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