CN108619560A - A kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film - Google Patents

A kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film Download PDF

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CN108619560A
CN108619560A CN201810521043.2A CN201810521043A CN108619560A CN 108619560 A CN108619560 A CN 108619560A CN 201810521043 A CN201810521043 A CN 201810521043A CN 108619560 A CN108619560 A CN 108619560A
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gelma
hemostatic
antibacterial
nanometer film
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CN108619560B (en
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施雪涛
陈云华
宣承楷
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South China University of Technology SCUT
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A61L24/104—Gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/001—Use of materials characterised by their function or physical properties
    • A61L24/0015—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
    • A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
    • A61L24/10—Polypeptides; Proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
    • A61L26/0028—Polypeptides; Proteins; Degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
    • A61L26/0028—Polypeptides; Proteins; Degradation products thereof
    • A61L26/0038—Gelatin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
    • A61L26/0061—Use of materials characterised by their function or physical properties
    • A61L26/0066—Medicaments; Biocides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
    • A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces

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  • Health & Medical Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
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Abstract

The invention discloses a kind of preparation methods of tissue adhension hemostatic and antibacterial nanometer film, including step：1) preparation of tissue adhension hemostatic and antibacterial basis material；2) that step 1) is obtained basis material is soluble in water, obtains nano thin-film by spin-coating method, then dries and removes solvent after UV photocurings, obtain required tissue adhension hemostatic and antibacterial nanometer film；Water soluble polymer is dissolved in water, sacrificial layer is built in nanometer film by spin-coating method, this layer of sacrificial layer facilitates practical operation and follow-up in use, can be removed by washing.The biological safety of tissue adhension hemostatic and antibacterial nanometer film obtained is good by the method for the invention, easy to use comfortable, and for energy secure adhesion in the tissue of different-shape, hemostatic and antibacterial effect is good and not will produce bacterial resistance.

Description

A kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film
Technical field
The present invention relates to the technical field of wound repair material, a kind of tissue adhension hemostatic and antibacterial nanometer film is referred in particular to Preparation method.
Background technology
Huge market and lasting demand, the research and development of soft tissue repair material are always bio-medical material The hot spot in field.Traditional soft-tissue trauma repair materials mainly have medical adhesive tape, suture and biological glue, they are invented certainly Since just always be the mankind existence and health make significant contribution.
Medical adhesive tape mainly has gauze, adhesive bandage etc., be now price it is most cheap, using most convenient, widest wound Wrapper material.But in the complicated surface of a wound of reply, show slightly weak on sealing effect.Closure strength of the operation suture thread to wound It is very high, and have successively develop degradable operation suture thread recently, cause patient's trouble and pain without postoperative dismounting.But Wound secondary damage and infection caused by when needing medical practitioner to be operated, and using, postoperative-scar is also inevitable 's.Commercial biological glue mainly has a-cyanoacrylate medical adhesive, can be used for skin surface or sub-layer wound repair, possesses solid The advantages that change speed is fast, and intensity is high, easy to use.But it is in brittleness after the solidification of cyanoacrylate biological glue, it is difficult to human body Organize modulus matching, when use that wound is caused to generate heat, degradation product has the shortcomings that cytotoxicity also limits its extensive use.
It is rare in view of being subsequently likely to occur moreover, traditional repair mode only stops blooding to being closed after wound debridement mostly A series of problems that bacterium infection is brought.Based on the flexibility that nano thin-film dimensional effect assigns, may be implemented to different-shape The surface of a wound carries out physical adhesion；Based on the good biocompatibility of gelatin modified object (GelMA) and bioactivity, we first will tool The DOPA of adhesiveness is amine-modified in a organized way arrives gelatin modified object skeleton, assigns its tissue adherence；Then that antibacterial effect is excellent, Bacterial resistance, the modification of avirulent antibacterial polypeptide are not generated to gelatin modified object skeleton, assign its antibiotic property；By with blood coagulation because Muon physics mix, and assign its coagulant property, finally obtain a kind of tissue adhension hemostatic and antibacterial basis material.By this basis material It is prepared into nano thin-film by spin-coating method, and structure facilitates the water soluble polymer sacrificial layer of practical operation on it, in use, This sacrificial layer can be removed by the method for washing, finally leave that tissue adherence is good, nanometer film of hemostatic and antibacterial in wound. This hemostatic and antibacterial film can be used for soft tissue repair, and a wide range of epidermis for being especially used to not suturing, adhesive tape can not be closed lacks Damage is repaired, it will is a kind of completely new, efficient scheme.
Invention content
The shortcomings that it is an object of the invention to overcome the prior art and deficiency, it is proposed that a kind of effective, scientific and reasonable Tissue adhension hemostatic and antibacterial nanometer film preparation method, pass through the life of tissue adhension hemostatic and antibacterial nanometer film made from this method Object safety is good, easy to use comfortable, and for energy secure adhesion in the tissue of different-shape, hemostatic and antibacterial effect is good and not will produce thin Bacterium resistance.
To achieve the above object, technical solution provided by the present invention is：A kind of tissue adhension hemostatic and antibacterial nanometer film Preparation method includes the following steps：
1) preparation of tissue adhension hemostatic and antibacterial basis material
1.1) methacrylic acid anhydridization gelatin GelMA is dissolved in water, it is 10-20%'s to be added and account for GelMA mass percents The N- hydroxysuccinimide NHS of 1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides EDC and 20-30%, and adjust It is 5.0~6.0 to save pH, is activated 30 minutes at 37 DEG C, obtains A systems；It is 50-70% that GelMA mass percents, which will then be accounted for, Dopamine hydrochloride DA A systems are added, react at least 12 hours, obtain sample B；By sample B dialysis, then freeze-drying obtains white To light grey solid, it is named as GelMA-DA；
1.2) GelMA-DA that step 1.1) obtains is dissolved in water, it is 2 to be charged with and account for GelMA-DA mass percents The dimethylacetylamide DMAC solution of the triphenylphosphine catalysis of~4% sulfydryl antibacterial polypeptide PT and 0.1~0.2%；Room temperature is lazy Terminate after being reacted 1 hour under property atmosphere, obtains sample C；By sample C dialysis, then freeze-drying obtains white to light grey solid, life Entitled GelMA-DA-PT；
1.3) by obtained by step 1.2) GelMA-DA-PT and account for the calcium chloride CaCl of its mass ratio 2.0~3.0%2 Physical mixed obtains product and is named as GelMA-DA-PT/CaCl2；
2) step 1.3) is obtained into GelMA-DA-PT/CaCl2It is soluble in water, with the molten of obtained 5~10% mass fractions Liquid, addition account for GelMA-DA-PT/CaCl2The initiator i2595 that mass fraction is 0.5~1.0%, is received by spin-coating method Rice film, dries and removes solvent after UV photocurings, obtains required tissue adhension hemostatic and antibacterial nanometer film.
By mass fraction be 20~30% 1788 type aqueous solution of PVAC polyvinylalcohol by spin-coating method, obtained in step 2) Tissue adhension hemostatic and antibacterial nanometer film on build one layer of sacrificial layer, to facilitate later stage practical operation, and follow-up in use, energy It is enough to be removed by washing.
In step 2), the thickness of tissue adhension hemostatic and antibacterial nanometer film obtained is 50nm~100nm.
Sacrificial layer thickness obtained is 0.2~0.5mm.
Compared with prior art, the present invention having the following advantages that and advantageous effect：
Can obtain by the method for the invention a kind of biological safety it is good, it is easy to use it is comfortable, can cope with the complex topography surface of a wound, Adhering soft tissues effect is good, hemostatic and antibacterial excellent effect and the film that not will produce bacterial resistance, can be used for avoiding because hurting, piercing Hinder class external form acute injury, bacterium infection caused by part visceral injury and diabetes class chronic wounds etc. has practical Promotional value.
Specific implementation mode
The present invention is further explained in the light of specific embodiments.
Embodiment 1
The preparation method for the tissue adhension hemostatic and antibacterial nanometer film that the present embodiment is provided, includes the following steps：
1) preparation of tissue adhension hemostatic and antibacterial basis material
1.1) GelMA is dissolved in water, the NHS for accounting for the EDC and 20% that its mass fraction is 10% is added, and adjust pH and be It is activated 30 minutes at 5.0,37 DEG C and obtains system A；The Dopamine hydrochloride that GelMA mass fractions are 50% will then be accounted for, system is added A, reaction obtain sample B at least 12 hours；By sample B dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA。
1.2) GelMA-DA that step 1.1) obtains is dissolved in water, is charged with that account for its mass fraction anti-for 2% sulfydryl The DMAC solution of the triphenylphosphine catalysis of bacterium polypeptide PT and 0.1%；Terminate to obtain sample after reacting 1 hour under room temperature inert atmosphere Product C；By sample C dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA-PT.
1.3) by obtained by step 1.2) GelMA-DA-PT and account for its mass fraction be 2.0% CaCl2Physical mixed, It obtains product and is named as GelMA-DA-PT/CaCl2。
2) step 1.3) is obtained into GelMA-DA-PT/CaCl2It is soluble in water, it is 5% solution with mass fraction is made, adds Enter to account for GelMA-DA-PT/CaCl2The initiator i2595 that mass fraction is 0.5% obtains nano thin-film, UV light by spin-coating method Solvent is dried and removed after solidification, obtains the tissue adhension hemostatic and antibacterial nanometer film that thickness is 50nm or so.
3) by mass fraction be 20% PVA1788 types aqueous solution by spin-coating method, in the tissue adhension that step 2) obtains The sacrificial layer of thickness 0.2mm or so is built in hemostatic and antibacterial nanometer film, this layer of sacrificial layer facilitates practical operation and subsequently make In, it can be removed by washing.
Embodiment 2
The preparation method for the tissue adhension hemostatic and antibacterial nanometer film that the present embodiment is provided, includes the following steps：
1) preparation of tissue adhension hemostatic and antibacterial basis material
1.1) GelMA is dissolved in water, the NHS for accounting for the EDC and 30% that its mass fraction is 20% is added, and adjust pH and be It is activated 30 minutes at 6.0,37 DEG C and obtains system A；The Dopamine hydrochloride that GelMA mass fractions are 75% will then be accounted for, system is added A, reaction obtain sample B at least 12 hours；By sample B dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA。
1.2) GelMA-DA that step 1.1) obtains is dissolved in water, is charged with that account for its mass fraction anti-for 4% sulfydryl The DMAC solution of the triphenylphosphine catalysis of bacterium polypeptide PT and 0.2%；Terminate to obtain sample after reacting 1 hour under room temperature inert atmosphere Product C；By sample C dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA-PT.
1.3) by obtained by step 1.2) GelMA-DA-PT and account for its mass fraction be 3.0% CaCl2Physical mixed, It obtains product and is named as GelMA-DA-PT/CaCl2。
2) step 1.3) is obtained into GelMA-DA-PT/CaCl2It is soluble in water, it is 10% solution with mass fraction is made, adds Enter to account for GelMA-DA-PT/CaCl2The initiator i2595 that mass fraction is 1.0% obtains nano thin-film, UV light by spin-coating method Solvent is dried and removed after solidification, obtains the tissue adhension hemostatic and antibacterial nanometer film that thickness is 100nm or so.
3) by mass fraction be 30% PVA1788 types aqueous solution by spin-coating method, in the tissue adhension that step 2) obtains A thickness 0.5mm or so sacrificial layer is built in hemostatic and antibacterial nanometer film, this layer of sacrificial layer facilitates practical operation and subsequently using In, it can be removed by washing.
Embodiment 3
The preparation method for the tissue adhension hemostatic and antibacterial nanometer film that the present embodiment is provided, includes the following steps：
1) preparation of tissue adhension hemostatic and antibacterial basis material
1.1) GelMA is dissolved in water, the NHS for accounting for the EDC and 25% that its mass fraction is 15% is added, and adjust pH and be It is activated 30 minutes at 5.5,37 DEG C and obtains system A；The Dopamine hydrochloride that GelMA mass fractions are 60% will then be accounted for, system is added A, reaction obtain sample B at least 12 hours；By sample B dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA。
1.2) GelMA-DA that step 1.1) obtains is dissolved in water, is charged with that account for its mass fraction anti-for 3% sulfydryl The DMAC solution of the triphenylphosphine catalysis of bacterium polypeptide PT and 0.15%；Terminate to obtain after reacting 1 hour under room temperature inert atmosphere Sample C；By sample C dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA-PT.
1.3) by obtained by step 1.2) GelMA-DA-PT and account for its mass fraction be 2.5% CaCl2Physical mixed, It obtains product and is named as GelMA-DA-PT/CaCl2。
2) step 1.3) is obtained into GelMA-DA-PT/CaCl2It is soluble in water, it is 7.5% solution with mass fraction is made, Addition accounts for GelMA-DA-PT/CaCl2The initiator i2595 that mass fraction is 0.75% obtains nano thin-film by spin-coating method, Solvent is dried and removed after UV photocurings, obtains the tissue adhension hemostatic and antibacterial nanometer film that thickness is 78nm or so.
3) by mass fraction be 25% PVA1788 types aqueous solution by spin-coating method, in the tissue adhension that step 2) obtains A thickness 0.35mm or so sacrificial layer is built in hemostatic and antibacterial nanometer film, this layer of sacrificial layer facilitates practical operation and subsequently using In, it can be removed by washing.
Embodiment described above is only the preferred embodiments of the invention, and but not intended to limit the scope of the present invention, therefore Change made by all shapes according to the present invention, principle, should all cover within the scope of the present invention.

Claims (4)

1. a kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film, which is characterized in that include the following steps：
1) preparation of tissue adhension hemostatic and antibacterial basis material
1.1) methacrylic acid anhydridization gelatin GelMA is dissolved in water, the 1- (3- for accounting for that GelMA mass percents are 10-20% is added Dimethylamino-propyl) -3- ethyl-carbodiimide hydrochlorides EDC and 20-30% N- hydroxysuccinimide NHS, and adjust pH It is 5.0~6.0, is activated 30 minutes at 37 DEG C, obtain A systems；The salt that GelMA mass percents are 50-70% will then be accounted for A systems are added in sour dopamine D A, react at least 12 hours, obtain sample B；Sample B dialysis and then freeze-drying are obtained white to light Gray solid is named as GelMA-DA；
1.2) GelMA-DA that step 1.1) obtains is dissolved in water, it is 2~4% to be charged with and account for GelMA-DA mass percents The dimethylacetylamide DMAC solution of the triphenylphosphine catalysis of sulfydryl antibacterial polypeptide PT and 0.1~0.2%；Room temperature inert atmosphere Lower reaction terminates after 1 hour, obtains sample C；By sample C dialysis, then freeze-drying obtains white to light grey solid, is named as GelMA-DA-PT；
1.3) by obtained by step 1.2) GelMA-DA-PT and account for the calcium chloride CaCl of its mass ratio 2.0~3.0%2Physics is mixed It closes, obtains product and be named as GelMA-DA-PT/CaCl2；
2) step 1.3) is obtained into GelMA-DA-PT/CaCl2It is soluble in water, with the solution that 5~10% mass fractions are made, it is added Account for GelMA-DA-PT/CaCl2The initiator i2595 that mass fraction is 0.5~1.0% obtains nano thin-film by spin-coating method, Solvent is dried and removed after UV photocurings, obtains required tissue adhension hemostatic and antibacterial nanometer film.
2. a kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film according to claim 1, it is characterised in that：By matter The 1788 type aqueous solution of PVAC polyvinylalcohol that score is 20~30% is measured by spin-coating method, is stopped in the tissue adhension that step 2) obtains One layer of sacrificial layer is built on blood antimicrobial nano film, to facilitate later stage practical operation, and follow-up in use, can be removed by washing It goes.
3. a kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film according to claim 1, it is characterised in that：In step It is rapid 2) in, the thickness of tissue adhension hemostatic and antibacterial nanometer film obtained is 50nm~100nm.
4. a kind of preparation method of tissue adhension hemostatic and antibacterial nanometer film according to claim 2, it is characterised in that：It is made Sacrificial layer thickness be 0.2~0.5mm.
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