CN108619551A - A kind of preparation method of antiseptic dressing - Google Patents
A kind of preparation method of antiseptic dressing Download PDFInfo
- Publication number
- CN108619551A CN108619551A CN201810593238.8A CN201810593238A CN108619551A CN 108619551 A CN108619551 A CN 108619551A CN 201810593238 A CN201810593238 A CN 201810593238A CN 108619551 A CN108619551 A CN 108619551A
- Authority
- CN
- China
- Prior art keywords
- stirred
- mass ratio
- take
- rotary evaporation
- mixed liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/20—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing organic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/26—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/204—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials with nitrogen-containing functional groups, e.g. aminoxides, nitriles, guanidines
- A61L2300/208—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of preparation methods of antiseptic dressing, belong to biomedicine field.The present invention using agarose as raw material, have excellent gelling properties, to protein without specific adsorption, have a characteristics such as significant stability, chlorosulfonic acid and anhydrous pyridine is added, by esterification, reactant is obtained, contains a large amount of hydroxyl in agarose, with sulfuric acid ester bond after reaction, all there is hydrophilicity, after being combined with the moisture in blood, it is absorbed by agarose molecules, instantaneous touch angle becomes smaller, and conjugation is got higher, and Fungicidal substance is added, bacteria breed is prevented, infection probability is reduced.The present invention solves the problems, such as current antiseptic dressing, and there are the laminating degree of antibacterial effect difference and skin is poor.
Description
Technical field
The invention belongs to biomedicine fields, and in particular to a kind of preparation method of antiseptic dressing.
Background technology
Medical dressing is the articles for use of packet wound, to cover the medical material of sore, wound or other damages.Medical dressing is made
For the covering of wound, it is made temporarily to play the partial action of skin barrier function, provides one and be conducive to wound healing
Environment waits for surface of a wound epithelialization or is transitioned into and rebuilds permanent skin barrier.With the pathologic, physiologic to wound
Further investigation, people are also more and more deep to the understanding of wound, to push continuously improving for Wound dressing
With development.
Today, novel Wound dressing relative to revolutionary variation is had occurred that in early days, from passive-type dressing, development
To coactive pattern dressing, followed by develop to biological active dressing now.Ideal Wound dressing should meet claimed below：It is anti-
The loss of sealing part and body fluid；Bacterial invasion is resisted, prevents from infecting；There is affinity with the surface of a wound, there is preferable bonding force；Keep,
Promote granulation and epithelial tissue normal growth, promotes recovery from illness；Preventing from scar is not deformed；Softness has certain mechanical strength；
Ventilative, moisture-inhibiting has moisture retention；Good biocompatibility；Comfortably, conveniently, it prepares and is easy, it is cheap.Flap coverage it is most ideal
Method be self-skin transplant, but it is often insufficient to transplant skin source.With the development of Polymeric Industry, prepared by synthetic material applies
Material is also increasing, substantially there is film class, liquid type, spray-like, foam class, Hydrogels etc..But in actual use
The problems such as generally existing antibacterial effect is undesirable poor with the laminating degree of skin.Therefore, producing a kind of can solve the above problems
Antiseptic dressing have prodigious market prospects.
Invention content
The technical problems to be solved by the invention：For current antiseptic dressing, there are the fittings of antibacterial effect difference and skin
The problem of degree difference provides a kind of preparation method of antiseptic dressing.
In order to solve the above technical problems, the present invention is using technical solution as described below：
A kind of preparation method of antiseptic dressing includes the following steps：
（1）Take agarose in mass ratio 1 ~ 3：0.8~1.5：10 ~ 30 are added fungicide, dry formamide mixing, are stirred in 45 ~ 55 DEG C
Mixing is mixed, mixture is obtained, anhydrous pyridine is taken to be stirred in -4 ~ -1 DEG C, adds the chlorosulfonic acid of 2 ~ 4 times of anhydrous pyridine quality,
It is stirred, filters, take filter residue, take mixture in mass ratio 1 ~ 4：2 ~ 5 addition filter residues are stirred, and are obtained stirring mixture, are taken
Stir mixture in mass ratio 1：3 ~ 5 are added absolute ethyl alcohol, and centrifugation takes precipitation to dialyse, and is evaporated under reduced pressure, and freeze-drying must freeze
Dried object, it is spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：7 ~ 10 addition dichloromethane are stirred, and add fluorenes methoxy
The 2,2'- of 3 ~ 5 times of carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, keep the temperature, rotary evaporation obtains rotary evaporation object；
（3）Take rotary evaporation object in mass ratio 1 ~ 3：0.3~0.5：10 ~ 15 addition methacrylic acids, dichloromethane are stirred,
Mixture A must be stirred, stirring mixture A in mass ratio 50 ~ 60 is taken：0.5~0.8：0.3 ~ 0.5 is added 1-（3- dimethylaminos third
Base）- 3- ethyl-carbodiimide hydrochlorides, N, N '-diisopropylcarbodiimide are stirred, and rotary evaporation obtains rotary evaporation object
A；
（4）Take step（1）Spare freeze-drying object in mass ratio 2 ~ 5：1~3：10 ~ 15 are added rotary evaporation object A, dimethyl Asia
Sulfone mixes, and is stirred 30 ~ 40min in 25 ~ 30 DEG C, adds the 1- of rotary evaporation object A mass 30 ~ 40%（3- dimethylaminos third
Base）The N of -3- ethyl-carbodiimide hydrochlorides and rotary evaporation object A mass 30 ~ 40%, N '-diisopropylcarbodiimide, in 25 ~
35 DEG C are stirred 5 ~ 7h, obtain mixture A, take mixture A in mass ratio 1：5 ~ 7 are added isopropanol, stand, take precipitation drying,
Obtain dried object；
（5）Take polycaprolactone in mass ratio 11 ~ 3：10~14：1 ~ 3 addition dichloromethane, dimethylformamide are stirred, then add
The ascorbyl palmitate for entering polycaprolactone quality 10 ~ 15%, is stirred 2 ~ 3h, obtains mixed liquor, takes dried object in mass ratio
1：10 ~ 13 are added distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, be placed in micro-injection
Spinning on pump, then be placed in mass fraction be 20% glutaraldehyde steam in keep 24 ~ 36h, under ultraviolet light keep 10 ~
20min, vacuum distillation are dried in vacuo to get antiseptic dressing.
The step（1）Middle fungicide is：Dodecyl trimethyl ammonium chloride, dodecyl benzyl dimethyl ammonium chloride,
Any one in 2,3- epoxypropyltrimethylchloride chlorides.
The step（1）It is middle stirring mixture formation condition be：Be stirred 1 ~ 2h in 55 ~ 65 DEG C, adjust pH to 7 ~
7.2。
The step（2）Middle rotary evaporation object formation condition is：In -1 ~ 2 DEG C keep the temperature 18 ~ for 24 hours.
The step（3）The formation condition of middle rotary evaporation object A is：It is protected from light in -1 ~ 2 DEG C and is stirred 6 ~ 8h.
The step（5）The service condition of mixed liquor and mixed liquor a in micro-injection pump：A points of mixed liquor and mixed liquor
It is not placed in and receives roller both sides, placed in 180o, mixed liquor and mixed liquor a flow velocitys are 8 μ L/min, syringe needle and receiver roller
The distance between be 10 ~ 15 cm.
The step（5）Middle spinning condition is：6 ~ 9h of spinning under the conditions of 25 ~ 30 DEG C, relative humidity 30 ~ 32%.
Compared with other methods, advantageous effects are the present invention：
（1）The present invention using agarose as raw material, have excellent gelling properties, to protein without specific adsorption, have it is significant
Chlorosulfonic acid and anhydrous pyridine is added in the characteristics such as stability, by esterification, obtains reactant, contains in agarose a large amount of
Hydroxyl all there is hydrophilicity to be inhaled by agarose molecules after being combined with the moisture in blood with sulfuric acid ester bond after reaction
It receives, instantaneous touch angle becomes smaller, and conjugation is got higher, and Fungicidal substance is added, and prevents bacteria breed, reduces infection probability；
（2）The present invention is with 2,2'-（Ethylene dioxy）It is double（Ethamine）, methacrylic acid be raw material, be added amino protecting agent fluorenes methoxy
Carbonyl acyl succinimide etc. prepares the small-molecule substance with cross-linking double bond by mono amino Protection Code, is grafted to above-mentioned
With agarose, a part for dressing is obtained, wherein having a large amount of OEG units so that the hydrone in blood is made by hydrogen bond
Hydrated sheath is formed to which as protein, in the barrier of adsorption, protein and biology can be effectively inhibited with being combined with dressing
The absorption of body, to good anti-protein adsorption performance, have stain resistance, while ensure that when more change dressings
Adhesion is not generated with wound, cause the secondary damage of wound, and mitigate feeling of pain, the gel structure of formation is conducive to the life of cell
Length, the entrance of oxygen, waste discharge and the invasion for preventing bacterium；
（3）It is raw material that polycaprolactone, which is added, with Vitamin C palmitate in the present invention, and another component is made and is added to antiseptic dressing
In, a kind of liposoluble vitamin of ascorbyl palmitate has stable inoxidizability, can accelerate human body fibroblast
The migration of tissue and proliferation are the confactors for generating hydroxyproline and hydroxylysine, so for the first kind and third class
The growth of collagen has preferable facilitation, the pride of polycaprolactone fiber such as to play the tensile strength that toughening effect improves dressing
And mechanical property, improve the overall performance of antiseptic dressing.
Specific implementation mode
Fungicide：Dodecyl trimethyl ammonium chloride, dodecyl benzyl dimethyl ammonium chloride, 2,3- glycidyl front threes
Any one in ammonium chloride.
A kind of preparation method of antiseptic dressing, includes the following steps：
（1）Take agarose in mass ratio 1 ~ 3：0.8~1.5：10 ~ 30 are added fungicide, dry formamide mixing, are stirred in 45 ~ 55 DEG C
20 ~ 30min of mixing is mixed, mixture is obtained, takes anhydrous pyridine to stir 20 ~ 30min in -4 ~ -1 DEG C, adds anhydrous pyridine quality 2 ~ 4
Chlorosulfonic acid again, is stirred 1 ~ 3h, filters, takes filter residue, take mixture in mass ratio 1 ~ 4：2 ~ 5 are added filter residue, in 55 ~ 65 DEG C
It is stirred 1 ~ 2h, pH to 7 ~ 7.2 is adjusted, obtains stirring mixture, takes stirring mixture in mass ratio 1：3 ~ 5 are added anhydrous second
Alcohol, centrifugation take precipitation dialysis 3 ~ 5 days, are evaporated under reduced pressure, and freeze-drying obtains freeze-drying object, spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：7 ~ 10 are added dichloromethane, are stirred 30 ~ 40min, then add
Enter the 2,2'- of 3 ~ 5 times of fluorenes methoxy carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, in -1 ~ 2 DEG C keep the temperature 18 ~ for 24 hours, rotation
Evaporation, obtains rotary evaporation object；
（3）Take rotary evaporation object in mass ratio 1 ~ 3：0.3~0.5：10 ~ 15 addition methacrylic acids, dichloromethane are stirred 1 ~
2h must stir mixture A, take stirring mixture A in mass ratio 50 ~ 60：0.5~0.8：0.3 ~ 0.5 is added 1-（3- dimethylaminos
Propyl）- 3- ethyl-carbodiimide hydrochlorides, N, N '-diisopropylcarbodiimide are protected from light in -1 ~ 2 DEG C and are stirred 6 ~ 8h, rotation
Turn evaporation, obtains rotary evaporation object A；
（4）Take step（1）Spare freeze-drying object in mass ratio 2 ~ 5：1~3：10 ~ 15 are added rotary evaporation object A, dimethyl Asia
Sulfone mixes, and is stirred 30 ~ 40min in 25 ~ 30 DEG C, adds the 1- of rotary evaporation object A mass 30 ~ 40%（3- dimethylaminos third
Base）The N of -3- ethyl-carbodiimide hydrochlorides and rotary evaporation object A mass 30 ~ 40%, N '-diisopropylcarbodiimide, in 25 ~
35 DEG C are stirred 5 ~ 7h, obtain mixture A, take mixture A in mass ratio 1：5 ~ 7 are added isopropanol, stand 8 ~ 10h, take precipitation
It is dry, obtain dried object；
（5）Take polycaprolactone in mass ratio 1 ~ 3：10~14：1 ~ 3 addition dichloromethane, dimethylformamide be stirred 30 ~
40min adds the ascorbyl palmitate of polycaprolactone quality 10 ~ 15%, is stirred 2 ~ 3h, obtain mixed liquor, takes drying
Object in mass ratio 1：10 ~ 13 are added distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, set
In on micro-injection pump, mixed liquor and mixed liquor a are respectively placed in and receive roller both sides, are placed in 180o, mixed liquor and mixed liquor a
Flow velocity is 8 μ L/min, and the distance between syringe needle and receiver roller are 10 ~ 15 cm, in 25 ~ 30 DEG C, relative humidity 30 ~
6 ~ 9h of spinning under the conditions of 32%, then be placed in the glutaraldehyde steam that mass fraction is 20% and keep 24 ~ 36h, then at ultraviolet light
10 ~ 20min of lower holding, vacuum distillation are dried in vacuo to get antiseptic dressing.
Fungicide：Dodecyl trimethyl ammonium chloride.
A kind of preparation method of antiseptic dressing, includes the following steps：
（1）Take agarose in mass ratio 1：0.8：10 are added fungicide, dry formamide mixing, and 20min is stirred in 45 DEG C,
Mixture is obtained, takes anhydrous pyridine to stir 20min in -4 DEG C, adds the chlorosulfonic acid of 2 times of anhydrous pyridine quality, be stirred 1h,
It filters, takes filter residue, take mixture in mass ratio 1：2 are added filter residue, and 1h is stirred in 55 DEG C, adjust pH to 7, must be stirred
Object takes stirring mixture in mass ratio 1：3 are added absolute ethyl alcohol, and centrifugation takes precipitation dialysis 3 days, is evaporated under reduced pressure, freeze-drying,
It must be freeze-dried object, it is spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：7 are added dichloromethane, are stirred 30min, add fluorenes first
The 2,2'- of 3 times of oxygen carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, 18h is kept the temperature in -1 DEG C, rotary evaporation must rotate steaming
Send out object；
（3）Take rotary evaporation object in mass ratio 1：0.3：10 addition methacrylic acids, dichloromethane are stirred 1h, must stir mixed
Object A is closed, stirring mixture A in mass ratio 50 is taken：0.5：0.3 is added 1-（3- dimethylamino-propyls）- 3- ethyl carbodiimide salt
Hydrochlorate, N, N '-diisopropylcarbodiimide are protected from light in -1 DEG C and are stirred 6h, rotary evaporation, obtain rotary evaporation object A；
（4）Take step（1）Spare freeze-drying object in mass ratio 2：1：10 are added rotary evaporation object A, dimethyl sulfoxide (DMSO) mixing,
It is stirred 30min in 25 DEG C, adds the 1- of rotary evaporation object A mass 30%（3- dimethylamino-propyls）- 3- ethyls carbon two is sub-
The N of amine hydrochlorate and rotary evaporation object A mass 30%, N '-diisopropylcarbodiimide are stirred 5h in 25 DEG C, obtain mixture
A takes mixture A in mass ratio 1：5 are added isopropanol, stand 8h, take precipitation drying, obtain dried object；
（5）Take polycaprolactone in mass ratio 1：10：1 addition dichloromethane, dimethylformamide are stirred 30min, add
The ascorbyl palmitate of polycaprolactone quality 10%, is stirred 2h, obtains mixed liquor, takes dried object in mass ratio 1：10 add
Enter distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, be placed on micro-injection pump, mixes
Liquid and mixed liquor a, which are respectively placed in, receives roller both sides, is placed in 180o, mixed liquor and mixed liquor a flow velocitys are 8 μ L/min, needle
The distance between head and receiver roller are 10cm, the spinning 6h under the conditions of 25 DEG C, relative humidity 30%, then are placed in quality point
Number for 20% glutaraldehyde steam in keep for 24 hours, 10min being kept under ultraviolet light, be evaporated under reduced pressure, vacuum drying to get
Antiseptic dressing.
Fungicide：Dodecyl benzyl dimethyl ammonium chloride.
A kind of preparation method of antiseptic dressing, includes the following steps：
（1）Take agarose in mass ratio 3：1.5：30 are added fungicide, dry formamide mixing, and 30min is stirred in 55 DEG C,
Mixture is obtained, takes anhydrous pyridine to stir 30min in -1 DEG C, adds the chlorosulfonic acid of 4 times of anhydrous pyridine quality, be stirred 3h,
It filters, takes filter residue, take mixture in mass ratio 4：5 are added filter residue, and 2h is stirred in 65 DEG C, adjust pH to 7.2, must stir mixed
Object is closed, stirring mixture in mass ratio 1 is taken：5 are added absolute ethyl alcohol, and centrifugation takes precipitation dialysis 5 days, is evaporated under reduced pressure, and freezing is dry
It is dry, freeze-drying object is obtained, it is spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：10 are added dichloromethane, are stirred 40min, add fluorenes
The 2,2'- of 5 times of methoxy carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, for 24 hours in 2 DEG C of heat preservations, rotary evaporation must rotate steaming
Send out object；
（3）Take rotary evaporation object in mass ratio 3：0.5：15 addition methacrylic acids, dichloromethane are stirred 2h, must stir mixed
Object A is closed, stirring mixture A in mass ratio 60 is taken：0.8：0.5 is added 1-（3- dimethylamino-propyls）- 3- ethyl carbodiimide salt
Hydrochlorate, N, N '-diisopropylcarbodiimide are protected from light in 2 DEG C and are stirred 8h, and rotary evaporation obtains rotary evaporation object A；
（4）Take step（1）Spare freeze-drying object in mass ratio 5：3：15 are added rotary evaporation object A, dimethyl sulfoxide (DMSO) mixing,
It is stirred 40min in 30 DEG C, adds the 1- of rotary evaporation object A mass 40%（3- dimethylamino-propyls）- 3- ethyls carbon two is sub-
The N of amine hydrochlorate and rotary evaporation object A mass 40%, N '-diisopropylcarbodiimide are stirred 7h in 35 DEG C, obtain mixture
A takes mixture A in mass ratio 1：7 are added isopropanol, stand 10h, take precipitation drying, obtain dried object；
（5）Take polycaprolactone in mass ratio 3：14：3 addition dichloromethane, dimethylformamide are stirred 40min, add
The ascorbyl palmitate of polycaprolactone quality 15%, is stirred 3h, obtains mixed liquor, takes dried object in mass ratio 1：13 add
Enter distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, be placed on micro-injection pump, mixes
Liquid and mixed liquor a, which are respectively placed in, receives roller both sides, is placed in 180o, mixed liquor and mixed liquor a flow velocitys are 8 μ L/min, needle
The distance between head and receiver roller are 15 cm, the spinning 9h under the conditions of 30 DEG C, relative humidity 32%, then are placed in quality point
Number for 20% glutaraldehyde steam in keep 36h, 20min is kept under ultraviolet light, be evaporated under reduced pressure, vacuum drying to get
Antiseptic dressing.
Fungicide：2,3- epoxypropyltrimethylchloride chlorides.
A kind of preparation method of antiseptic dressing, includes the following steps：
（1）Take agarose in mass ratio 2：1.1：20 are added fungicide, dry formamide mixing, and 25min is stirred in 50 DEG C,
Mixture is obtained, takes anhydrous pyridine to stir 25min in -2 DEG C, adds the chlorosulfonic acid of 3 times of anhydrous pyridine quality, be stirred 2h,
It filters, takes filter residue, take mixture in mass ratio 3：4 are added filter residue, and 1h is stirred in 60 DEG C, adjust pH to 7, must be stirred
Object takes stirring mixture in mass ratio 1：4 are added absolute ethyl alcohol, and centrifugation takes precipitation dialysis 4 days, is evaporated under reduced pressure, freeze-drying,
It must be freeze-dried object, it is spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：8 are added dichloromethane, are stirred 35min, add fluorenes first
The 2,2'- of 4 times of oxygen carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, 21h is kept the temperature in -1 DEG C, rotary evaporation must rotate steaming
Send out object；
（3）Take rotary evaporation object in mass ratio 2：0.4：13 addition methacrylic acids, dichloromethane are stirred 1h, must stir mixed
Object A is closed, stirring mixture A in mass ratio 55 is taken：0.7：0.4 is added 1-（3- dimethylamino-propyls）- 3- ethyl carbodiimide salt
Hydrochlorate, N, N '-diisopropylcarbodiimide are protected from light in 1 DEG C and are stirred 7h, and rotary evaporation obtains rotary evaporation object A；
（4）Take step（1）Spare freeze-drying object in mass ratio 3：2：13 are added rotary evaporation object A, dimethyl sulfoxide (DMSO) mixing,
It is stirred 35min in 27 DEG C, adds the 1- of rotary evaporation object A mass 35%（3- dimethylamino-propyls）- 3- ethyls carbon two is sub-
The N of amine hydrochlorate and rotary evaporation object A mass 35%, N '-diisopropylcarbodiimide are stirred 6h in 30 DEG C, obtain mixture
A takes mixture A in mass ratio 1：6 are added isopropanol, stand 9h, take precipitation drying, obtain dried object；
（5）Take polycaprolactone in mass ratio 2：12：2 addition dichloromethane, dimethylformamide are stirred 35min, add
The ascorbyl palmitate of polycaprolactone quality 13%, is stirred 2h, obtains mixed liquor, takes dried object in mass ratio 1：12 add
Enter distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, be placed on micro-injection pump, mixes
Liquid and mixed liquor a, which are respectively placed in, receives roller both sides, is placed in 180o, mixed liquor and mixed liquor a flow velocitys are 8 μ L/min, needle
The distance between head and receiver roller are 13cm, the spinning 7h under the conditions of 27 DEG C, relative humidity 31%, then are placed in quality point
Number for 20% glutaraldehyde steam in keep 30h, 15min is kept under ultraviolet light, be evaporated under reduced pressure, vacuum drying to get
Antiseptic dressing.
Comparative example：The antiseptic dressing of Nanjing company production.
The biological antibacterial dressing obtained respectively with comparative example to embodiment is detected, and is pressed《Drug Micro biological Tests handbook》
It is tested, measurement result is referring to table 1.
Table 1：
| Test event | Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example |
| Staphylococcus aureus bacteriostasis rate（%） | 95 | 96 | 95 | 78~86 |
| Escherichia coli bacteriostasis rate（%） | 90 | 94 | 93 | 80~85 |
| Candida albicans bacteriostasis rate（%） | 92 | 94 | 95 | 83~86 |
In summary, antiseptic dressing of the present invention has obvious inhibiting effect to gold-coloured staphylococci, Escherichia coli, Candida albicans.
Claims (7)
1. a kind of preparation method of antiseptic dressing, which is characterized in that the preparation method includes the following steps：
（1）Take agarose in mass ratio 1 ~ 3：0.8~1.5：10 ~ 30 are added fungicide, dry formamide mixing, are stirred in 45 ~ 55 DEG C
Mixing is mixed, mixture is obtained, anhydrous pyridine is taken to be stirred in -4 ~ -1 DEG C, adds the chlorosulfonic acid of 2 ~ 4 times of anhydrous pyridine quality,
It is stirred, filters, take filter residue, take mixture in mass ratio 1 ~ 4：2 ~ 5 addition filter residues are stirred, and are obtained stirring mixture, are taken
Stir mixture in mass ratio 1：3 ~ 5 are added absolute ethyl alcohol, and centrifugation takes precipitation to dialyse, and is evaporated under reduced pressure, and freeze-drying must freeze
Dried object, it is spare；
（2）Take fluorenes methoxy carbonyl acyl succinimide in mass ratio 1：7 ~ 10 addition dichloromethane are stirred, and add fluorenes methoxy
The 2,2'- of 3 ~ 5 times of carbonyl acyl succinimide（Ethylene dioxy）It is double（Ethamine）, keep the temperature, rotary evaporation obtains rotary evaporation object；
（3）Take rotary evaporation object in mass ratio 1 ~ 3：0.3~0.5：10 ~ 15 addition methacrylic acids, dichloromethane are stirred,
Mixture A must be stirred, stirring mixture A in mass ratio 50 ~ 60 is taken：0.5~0.8：0.3 ~ 0.5 is added 1-（3- dimethylaminos third
Base）- 3- ethyl-carbodiimide hydrochlorides, N, N '-diisopropylcarbodiimide are stirred, and rotary evaporation obtains rotary evaporation object
A；
（4）Take step（1）Spare freeze-drying object in mass ratio 2 ~ 5：1~3：10 ~ 15 are added rotary evaporation object A, dimethyl Asia
Sulfone mixes, and is stirred 30 ~ 40min in 25 ~ 30 DEG C, adds the 1- of rotary evaporation object A mass 30 ~ 40%（3- dimethylaminos third
Base）The N of -3- ethyl-carbodiimide hydrochlorides and rotary evaporation object A mass 30 ~ 40%, N '-diisopropylcarbodiimide, in 25 ~
35 DEG C are stirred 5 ~ 7h, obtain mixture A, take mixture A in mass ratio 1：5 ~ 7 are added isopropanol, stand, take precipitation drying,
Obtain dried object；
（5）Take polycaprolactone in mass ratio 1 ~ 3：10~14：1 ~ 3 addition dichloromethane, dimethylformamide are stirred, then add
The ascorbyl palmitate for entering polycaprolactone quality 10 ~ 15%, is stirred 2 ~ 3h, obtains mixed liquor, takes dried object in mass ratio
1：10 ~ 13 are added distilled water, obtain mixed liquor a, take mixed liquor and mixed liquor a to suck respectively in 5mL syringes, be placed in micro-injection
Spinning on pump, then be placed in mass fraction be 20% glutaraldehyde steam in keep 24 ~ 36h, under ultraviolet light keep 10 ~
20min, vacuum distillation are dried in vacuo to get antiseptic dressing.
2. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（1）Middle fungicide is：
It is arbitrary in dodecyl trimethyl ammonium chloride, dodecyl benzyl dimethyl ammonium chloride, 2,3- epoxypropyltrimethylchloride chlorides
It is a kind of.
3. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（1）In be stirred
The formation condition of object is：It is stirred 1 ~ 2h in 55 ~ 65 DEG C, adjusts pH to 7 ~ 7.2.
4. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（2）Middle rotary evaporation
Object formation condition is：In -1 ~ 2 DEG C keep the temperature 18 ~ for 24 hours.
5. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（3）Middle rotary evaporation
The formation condition of object A is：It is protected from light in -1 ~ 2 DEG C and is stirred 6 ~ 8h.
6. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（5）Mixed liquor and mixed
Close service conditions of the liquid a in micro-injection pump：Mixed liquor and mixed liquor a, which are respectively placed in, receives roller both sides, is placed in 180o,
Mixed liquor and mixed liquor a flow velocitys are 8 μ L/min, and the distance between syringe needle and receiver roller are 10 ~ 15 cm.
7. the preparation method of antiseptic dressing according to claim 1, which is characterized in that the step（5）Middle spinning condition
For：6 ~ 9h of spinning under the conditions of 25 ~ 30 DEG C, relative humidity 30 ~ 32%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810593238.8A CN108619551A (en) | 2018-06-11 | 2018-06-11 | A kind of preparation method of antiseptic dressing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810593238.8A CN108619551A (en) | 2018-06-11 | 2018-06-11 | A kind of preparation method of antiseptic dressing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108619551A true CN108619551A (en) | 2018-10-09 |
Family
ID=63691479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810593238.8A Withdrawn CN108619551A (en) | 2018-06-11 | 2018-06-11 | A kind of preparation method of antiseptic dressing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108619551A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110003362A (en) * | 2019-04-23 | 2019-07-12 | 福建省绿麒食品胶体有限公司 | A kind of preparation method of the modified agar of high grade of transparency high-gel strength |
-
2018
- 2018-06-11 CN CN201810593238.8A patent/CN108619551A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110003362A (en) * | 2019-04-23 | 2019-07-12 | 福建省绿麒食品胶体有限公司 | A kind of preparation method of the modified agar of high grade of transparency high-gel strength |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Lei et al. | Optimization of human-like collagen composite polysaccharide hydrogel dressing preparation using response surface for burn repair | |
| Patil et al. | Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability | |
| CN104623718B (en) | Chitosan petrolatum gauze and preparation method thereof | |
| CN101693121B (en) | Method for preparing hydrogel dressing with half interpenetrating network structure and application | |
| CN106377791A (en) | Bamboo alginate functional dressing and preparation method thereof | |
| CN103159967B (en) | Preparation method of collagen-based sponge wound dressing with self-anti-inflammatory function | |
| CN103755965A (en) | Epsilon-polylysine hydrogel and preparation method and application thereof | |
| CN113289050B (en) | Hemostatic sponge and preparation method thereof | |
| CN112480434A (en) | Copper ion antibacterial hydrogel and preparation method and application thereof | |
| CN103120803A (en) | Preparation method of bacterial cellulose composite chitosan moist antimicrobial dressing | |
| CN103848926A (en) | Preparation method and applications of carboxylation chitosan | |
| CN106344954A (en) | Bio-antimicrobial bacterial cellulose dressing and preparation method thereof | |
| Cui et al. | A chitosan-based self-healing hydrogel for accelerating infected wound healing | |
| CN111228296A (en) | Cross-linked hyaluronic acid ectoine isotonic wound flushing fluid | |
| CN108245700A (en) | A kind of hydroxypropyl methyl cellulose chitosan film dressing and preparation method thereof | |
| CN105288698A (en) | Calcium alginate composite medical surgical dressing and preparation method thereof | |
| CN108619551A (en) | A kind of preparation method of antiseptic dressing | |
| KR102333779B1 (en) | Protruded polyurethane dressing foam using nitric oxide plasma and its manufacturing method | |
| CN100384485C (en) | Microbiological cellulose injury dressing for treating chronic injury | |
| CN114479124B (en) | Self-healing hydrogel, preparation method and application thereof | |
| CN105879102B (en) | A kind of feather keratin grafting alginic acid sponge dressing and preparation method thereof | |
| CN112472867A (en) | Antibacterial medical hydrogel dressing and preparation method thereof | |
| CN113512132A (en) | Quick hemostatic hydrogel and preparation method thereof | |
| CN106937941A (en) | A kind of medical skin diminishing inflammation of wound gel and preparation method thereof | |
| CN112891605A (en) | Preparation method of ozone alginate antibacterial functional wound dressing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20181009 |