CN108618753A - A method of continuing Tensile stress stimulation and repairs rabbit rupture of achilles tendon - Google Patents
A method of continuing Tensile stress stimulation and repairs rabbit rupture of achilles tendon Download PDFInfo
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- CN108618753A CN108618753A CN201710177771.1A CN201710177771A CN108618753A CN 108618753 A CN108618753 A CN 108618753A CN 201710177771 A CN201710177771 A CN 201710177771A CN 108618753 A CN108618753 A CN 108618753A
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Abstract
Method that repairing rabbit rupture of achilles tendon is stimulated by lasting Tensile stress the present invention relates to a kind of, including：Using new zealand male rabbit, rabbit heel string acute rupture animal model is created by hind leg Tendon achillis art；The ankle-joint for fixing rabbit with gypsum in function digit, limits rabbit ankle moving, gives heel string disconnected section sustained stress stimulation, establishes the animal model of the lower heel string reparation of early stage sustained stress stimulation；Under being fixed by gross examination of skeletal muscle and the clear ankle joint function position of microscopic observation, rabbit heel string acute rupture repairs the histological change of early postoperation；Using type i collagen, TGF β 1,1 protein expression situation of bFGF, VEGF, PDGF and IGF in ELISA method detection repair tissue；By measuring maximum intensity, maximum value load and stiffness index, repair tissue biomechanical characterization after giving the stimulation of early stage sustained stress in the postoperative repair process of rabbit heel string acute rupture is evaluated.
Description
Technical field
The present invention relates to recovery technique more particularly to a kind of methods for continuing Tensile stress stimulation and repairing rabbit rupture of achilles tendon.
Background technology
Tendon injury is a kind of common damage, after reparation it occur frequently that adhesion of tendon or anchylosis and cause serious
Dysfunction.Domestic and foreign scholars focus primarily upon repairing for the improvement of sewing method and stndon sheath in art to the research of tendon repair at present
In multiple and replacement, but the functional rehabilitation that various therapies are still not up to satisfied.It is found in long-term clinical practice, tendon is repaiied
Although multiple postoperative Tendon loose position gypsum is fixed reduces the chance that tendon is broken again most possibly, flesh will also result in
Tendon contracture or anchylosis, substantially increase the difficulty and rehabilitation duration of later stage functional training, or even cause permanent work(
Energy obstacle, and there is the risks that higher tendon is broken again for early functional exercise.It can be by the postoperative relaxation of tendon suture
Position gypsum fixation is changed to plaster bandage in functional position and fixes and will generate strong influence to the difficulty or ease of later stage functional training and effect.
Invention content
It is existing to solve the purpose of the present invention is to provide a kind of method for continuing Tensile stress stimulation and repairing rabbit rupture of achilles tendon
There is the problem of postoperative relaxation position gypsum fixation of tendon suture can not being changed to plaster bandage in functional position present in technology.
The present invention provides a kind of method stimulating reparation rabbit rupture of achilles tendon by lasting Tensile stress, including：Using new west
Blue male rabbit creates rabbit heel string acute rupture animal model by hind leg Tendon achillis art；Rabbit is fixed with gypsum in function digit
Ankle-joint, limit rabbit ankle moving, give heel string and break the stimulation of section sustained stress, establish the lower heel string of early stage sustained stress stimulation
The animal model of reparation；Under being fixed by gross examination of skeletal muscle and the clear ankle joint function position of microscopic observation, rabbit heel string acute rupture is repaiied
The histological change of multiple early postoperation；Using type i collagen, TGF-β 1, bFGF, VEGF, PDGF in ELISA method detection repair tissue
With IGF-1 protein expression situations；By measuring maximum intensity, maximum value load and stiffness index, evaluation rabbit heel string is acute disconnected
Split repair tissue biomechanical characterization after giving the stimulation of early stage sustained stress in postoperative repair process.
The beneficial effects of the present invention are：Sustained stress stimulation pair caused by being fixed by ankle joint function position of analyzing and researching
The influence and tendon biomethanics of tissue culture and relevant growth factors expression in the postoperative repair process of rabbit heel string acute rupture
The variation of characteristic, the tissue repair mechanisms under being stimulated according to tendon acute rupture early postoperation sustained stress.It is disconnected to provide a kind of tendon
Split the fixed new method of gypsum after prosthetic.
Description of the drawings
The present invention is described in detail by following preferred embodiments and attached drawing for ease of explanation,.
Fig. 1 is that the method flow diagram for repairing rabbit rupture of achilles tendon is stimulated by lasting Tensile stress.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with attached drawing to the present invention
Embodiment be described in detail.
Tendon injury is a kind of clinically common damage, after reparation it occur frequently that adhesion of tendon or anchylosis and cause
Serious dysfunction.Domestic and foreign scholars focus primarily upon the improvement of sewing method and tendon in art to the research of tendon repair at present
In the reparation and replacement of sheath, but satisfied effect is not obtained always about postoperative functional rehabilitation.In addition rupture of tendon is most common
It in young people, is affected, should attach the importance to family, society.
We have found in long-term clinical practice, although the postoperative Tendon loose position gypsum of tendon repair fix maximum can
Energy ground reduces the chance that tendon is broken again, but will also result in tendon contracture or anchylosis, substantially increases later stage work(
The difficulty and rehabilitation duration that can be taken exercise, or even cause permanent dysfunction.System after being repaired some researches show that rupture of tendon
Dynamic that tendon will be made to be in appraxia state, the tensile strength of tendon tissue is substantially reduced.Killian ML etc. have found such as under study for action
The load that fruit completely removes the disconnected section spot of tendon will be unfavorable for healing process of tendons, and healing tendon tissue can be limited by completely removing load
Growth with structure simultaneously reduces tendon intensity.With new zealand white rabbit research braking to rabbit knee anterior cruciate ligament biology
Can it was found that, experiment each group rabbit fix 6 weeks in gypsum respectively and after 8 weeks dismounting gypsum after, the preceding friendship of each experimental group
Fork ligament maximum load, maximum tension length and maximum stress, maximum strain, elasticity modulus are more normally organized and become smaller.Thus it opens
Beginning has using gypsum fix postoperative to tendon suture of scholar to generate query.
With the development of tendon suture material, the continuous improvement of tendon suture technology, part hand surgeon begins attempt to
Early postoperative period functional training come prevent adhesion of tendon, promote tendon immediate union.Pass through opponent's extensor tendon injury repair art
Patient gives tendon disconnected section one early stage stress stimulation afterwards, is found with the comparison of delay activity group within postoperative 6th week and the 8th week, total master
Dynamic range-of-motion evaluation and functional independence scoring are apparently higher than control group, to illustrate that the stimulation of early stage sustained stress is cured conducive to tendon
It closes.However in early functional exercise using exercise method it is improper, or not health care professional instruct under fail very accurately
The intensity for holding strength, causes the early ambulant after tendon suture to have higher fracture rate again.Work early stage tendon repair
In dynamic, highest has 46% fracture rate in document.The adjustment of daily function exercise and brace also increases clinician's simultaneously
Workload and psychological pressure, this is also that current most hand surgeon still uses relaxation position gypsum system after tendon suture
The main reason for dynamic method.
Since traditional non-functional position gypsum fixes the chance of the adhesion and anchylosis that increase tendon, and early stage is applied
Functional brace and active and passive functions temper the dynamic stress stimulation generated again due to the uncontrollability of strength size, exist
The risk that tendon is broken again.So it is contemplated that the tendon after suture is fixed using plaster bandage in functional position, to avoid non-functional
Position gypsum fixes the tendon contracture and anchylosis brought, and reduces the difficulty and rehabilitation duration of later stage functional training.It can keep away again
Exempt from because of the risk that early functional exercise causes tendon to be broken again.But the Tensile stress of the lasting static state generated therefrom pierces
The healing of tendon can be had an impact by swashing.By being found after literature search and inspection information, Julius Wolff were delivered in 1892
's《The law of bone conversion》It elaborates：Bone tissue has a very strong adaptability to its mechanical environment, the form of bone and function it is every
A kind of variation or the individually variation of function, will lead to the variation of corresponding internal structure and outer shape, variation follows mathematics
Rule, that is to say, that the growth of bone can be influenced by mechanical stimulation and change its structure.Then strong with it, use of giving up is then weak, i.e.,
Wolff laws.Ilizarov etc. has found when studying distraction osteogenesis, drawing-off lasting to living tissue, stable, slow,
Certain histiocytic regeneration and active growth can be stimulated or activate, growth pattern is consistent with fetal tissue, is identical thin
Born of the same parents divide, i.e., famous Ilizarov tension-stress rule.Same rule is suitable for all connectives and connects tissue, tendon master
It to be made of dense connective tissue, so this law is also applied for tendon.The adaptability reconstruction institute occurred under this stress
Purpose to be achieved be exactly " most properly position is used in the material most saved, maximum load is carried with minimum deformation,
To drive most economical effective movement ".That is, more than compression can promote connective tissue growth, lasting, static leads
Tensile stress can similarly promote connective tissue growth.It is multiple that the reconstruction of stress undertissue is related to molecule, cell, histoorgan
Level, process is complicated, although proposing that over one hundred year, the biomechanism of wolff laws behind are complete not yet away from the present from earliest
It is clear, have more and more researchs and start to notice the effect that various growth factors play wherein, this may be pierced with machinery
It is related to swash the cell signalling caused.Mechanical signal can stimulate the stretching receptor and attachment sites of cell surface, lead to a system
Row water fall effect, nutritional ingredient, oxygen etc. to change cell peripheral.The second messenger N0 in Tenocyte cell can also be changed
Or Ca2+ concentration, the expression of cytokines mRNA is directly or indirectly influenced, to influence the synthesis [12] of stromatin.At present
That knows mainly has with relevant growth factor in healing process of tendons repair process：Transforming growth factor-β（transforming
Growth factor- β, TGF-β）, insulin-like growth factor（Insulin-like growth factor, IGF）, blood it is small
Plate source property growth factor（Platelet-derived growth factor, PDGF）, exogenous basic fibroblast growth
The factor（Basic fibroblast growth factor, bFGF）, vascular endothelial growth factor (vascular
Endothelial growth factor, VEGF) etc..Therefore, after tendon injury prosthetic, early stage carries out stress stimulation, can be with
Promote cytokine-expressing, promotes Tenocyte cell proliferation, improve the expression of collagen, promote the Complicated primary of tendon structure.
Based on the principles above present invention will by establishing plaster bandage in functional position fixed model after rabbit heel string acute injury prosthetic,
According to Wolff laws and Ilizarov tension-stress rule, the embodiment of the present invention repairs early postoperation because of function according to rabbit heel string
The influence for continuing biomethanics and relevant growth factors expression of the Tensile stress stimulation to heel string caused by position is fixed, provides one kind
The method for repairing rabbit rupture of achilles tendon is stimulated by lasting Tensile stress.
Fig. 1 is that the method flow diagram for repairing rabbit rupture of achilles tendon is stimulated by lasting Tensile stress.
The present invention stimulates the method for repairing rabbit rupture of achilles tendon to include the following steps by lasting Tensile stress：
Step 100, using new zealand male rabbit, rabbit heel string acute rupture animal model is created by hind leg Tendon achillis art.
Step 200, the ankle-joint of rabbit is fixed with gypsum in function digit, limits rabbit ankle moving, the disconnected section of heel string is given and holds
Continuous stress stimulation establishes the animal model of the lower heel string reparation of early stage sustained stress stimulation.
Step 300, under by gross examination of skeletal muscle and under the microscope, clear ankle joint function position is fixed, rabbit heel string acute rupture is repaiied
The histological change of multiple early postoperation.
Step 400, using type i collagen, TGF-β 1, bFGF, VEGF, PDGF and IGF-1 in ELISA method detection repair tissue
Protein expression situation.
Step 500, by measuring maximum intensity, maximum value load and stiffness index, rabbit heel string acute rupture art is evaluated
Given in repair process afterwards early stage sustained stress stimulation after repair tissue biomechanical characterization.
The beneficial effects of the present invention are：Sustained stress stimulation pair caused by being fixed by ankle joint function position of analyzing and researching
The influence and tendon biomethanics of tissue culture and relevant growth factors expression in the postoperative repair process of rabbit heel string acute rupture
The variation of characteristic, the tissue repair mechanisms under being stimulated according to tendon acute rupture early postoperation sustained stress.It is disconnected to provide a kind of tendon
Split the fixed new method of gypsum after prosthetic.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, for the skill of this field
For art personnel, the invention may be variously modified and varied.All within the spirits and principles of the present invention, any made by repair
Change, equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (1)
1. a kind of stimulating the method for repairing rabbit rupture of achilles tendon by lasting Tensile stress, which is characterized in that including：
Using new zealand male rabbit, rabbit heel string acute rupture animal model is created by hind leg Tendon achillis art；
The ankle-joint for fixing rabbit with gypsum in function digit, limits rabbit ankle moving, gives heel string disconnected section sustained stress stimulation, builds
The animal model of the vertical lower heel string reparation of early stage sustained stress stimulation；
Under being fixed by gross examination of skeletal muscle and the clear ankle joint function position of microscopic observation, rabbit heel string acute rupture repairs early postoperation
Histological change；
Using type i collagen, TGF-β 1, bFGF, VEGF, PDGF and IGF-1 protein expression feelings in ELISA method detection repair tissue
Condition；
By measuring maximum intensity, maximum value load and stiffness index, evaluate in the postoperative repair process of rabbit heel string acute rupture
Give early stage sustained stress stimulation after repair tissue biomechanical characterization.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710177771.1A CN108618753A (en) | 2017-03-23 | 2017-03-23 | A method of continuing Tensile stress stimulation and repairs rabbit rupture of achilles tendon |
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| CN201710177771.1A CN108618753A (en) | 2017-03-23 | 2017-03-23 | A method of continuing Tensile stress stimulation and repairs rabbit rupture of achilles tendon |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109381478A (en) * | 2018-10-31 | 2019-02-26 | 青岛大学附属医院 | A kind of miRNA inhibits new vessels to generate and inhibit the application in the reagent of VEGF-A factor expression in preparation |
-
2017
- 2017-03-23 CN CN201710177771.1A patent/CN108618753A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109381478A (en) * | 2018-10-31 | 2019-02-26 | 青岛大学附属医院 | A kind of miRNA inhibits new vessels to generate and inhibit the application in the reagent of VEGF-A factor expression in preparation |
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