CN1085954A - The method of extraction of glutamic acid from concentrated bacteria-contained fermentation liquor - Google Patents
The method of extraction of glutamic acid from concentrated bacteria-contained fermentation liquor Download PDFInfo
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- CN1085954A CN1085954A CN 92112240 CN92112240A CN1085954A CN 1085954 A CN1085954 A CN 1085954A CN 92112240 CN92112240 CN 92112240 CN 92112240 A CN92112240 A CN 92112240A CN 1085954 A CN1085954 A CN 1085954A
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- glutamic acid
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Abstract
Present method comprises fermented liquid iso-electric point extraction L-glutamic acid, obtains glutamic acid crystallization, the condensing crystal mother liquor, and filtering and concentrating liquid directly extracts from concentrated solution and obtains the high L-glutamic acid of purity.The invention reside in the fermented liquid of the L-glutamic acid of extracting section that concentrates mycetome, thickening equipment is selected falling film type film evaporator for use, drops into defoamer.
Description
In the glutamate production process, have from the step of broth extraction L-glutamic acid, fermented liquid is industrialness production, and by starch, molasses are raw material, through microorganism culturing fermentative preparation gained, method of enrichment is extracted L-glutamic acid and is compared with existing various extraction L-glutamic acid methods, and advantage is the yield height, can also collect proteinaceous thalline, the most of evaporation of the moisture content of fermented liquid, the concentrated solution of last gained are easy to handle and are made as fertilizer.
When but method of enrichment is always extracted L-glutamic acid, at first will be the thalline Impurity removal of fermented liquid, the clear liquid that obtains just enters concentrated, remove thalline impurity earlier, always there is the supercentrifuge of employing to separate, inorganic membrane filtration, but these methods are all because consumption of electric energy is big, and complicated operation, the reasonable enterprise of the condition that can only be confined to uses.
Patent of invention CN1048702A is that method of enrichment is extracted L-glutamic acid, and the foam that produces for fear of concentrated broth the time disturbs, and also is to use flocculation agent, removes thalline impurity earlier, but also has throw out to filter the difficulty of trouble.
Purpose of the present invention is to avoid existing method of enrichment to extract the deficiency of L-glutamic acid, simplifies processing step, can realize that also the method for enrichment of suitability for industrialized production is extracted L-glutamic acid.
Purpose of the present invention can reach by following steps, cultivate in the sophisticated fermented liquid by L-glutamic acid and to extract glutamic acid crystallization, comprise fermented liquid iso-electric point extraction L-glutamic acid, choosing is fit to concentration ratio, the condensing crystal mother liquor, filtering and concentrating liquid, iso-electric point is extracted L-glutamic acid from concentrated solution, the fermented liquid that it is characterized in that the L-glutamic acid of extracting section of concentrated mycetome is adjusted the L-glutamic acid content that is concentrated liquid and is restrained/100 milliliters 1.7～2.8, or spissated liquid, L-glutamic acid content is adjusted at/100 milliliters of 4.5～8.5 grams, concentration evaporator is an one-pass type flow evaporation device, 50 ℃～85 ℃ of temperature, vacuum tightness 0.06Mpa～0.1Mpa, drop into defoamer, in (bubble enemy is no more than 0.04%, or methyl-silicone oil is no more than 0.04%, or vegetables oil is no more than 0.4%) any.
One-pass type flow evaporation device is triple effect rising-film evaporator (or falling film evaporator).
The invention has the advantages that the one-pass type flow evaporation device of selecting for use, thickening equipment than other type is more conducive to overcome the foamy influence that concentration process produces, drop into defoamer such as silicone oil in the concentration process and can remove the foam that concentration process produces, the fermented liquid of extracting section L-glutamic acid enters concentrated at suitable L-glutamic acid content when selecting best concentration ratio to adjust, with the L-glutamic acid content that adjust to concentrate the concentrated solution that finishes in suitable concentration, help from concentrated solution the glutamic acid crystallization that extraction easily obtains well-crystallized.
The present invention is the improvement of patent of invention CN1048702A, has omitted the step of fermented liquid removal thalline impurity, embodies operational path of the present invention, and more reasonable than original not improved patent of invention, facility investment can reduce 1/4.
The non-limiting examples that will narrate by face not is further elaborated the present invention.
Embodiment one:
Get L-glutamic acid and cultivate sophisticated fermented liquid, iso-electric point gets the mycetome fermented liquid of extracting section L-glutamic acid after extracting L-glutamic acid, its L-glutamic acid content is/100 milliliters of 1.5 grams, drops into the fermented liquid that contains/100 milliliters of amount of glutamic acid 6.5 grams, makes the liquid L-glutamic acid content that is concentrated reach/100 milliliters of 2.2 grams, getting 20 tons of these liquid enters concentrated, select reverse-flow triple-effect evaporator, 50 ℃ of the temperature of triple effect, vacuum tightness 0.1Mpa, 70 ℃ of second temperature of imitating, vacuum tightness 0.08Mpa, 85 ℃ of first temperature of imitating, vacuum tightness 0.06Mpa, concentration ratio 3: 1, drop into defoamer any (silicone oil 0.04%, bubble enemy 0.04%, vegetables oil 0.4%), get 6.6 tons of concentrated solutions, concentrated solution is heated to 80 ℃, drops into 15 kilograms of gacs, through pressure filter filter clear liquid, clear liquid is through iso-electric point, PH3, cooling, freezing, extract 350 kilograms in L-glutamic acid, purity 90%, its secondary crystal mother liquor L-glutamic acid content is/100 milliliters of 1.8 grams, behind this mother liquor reconcentration with in the ammoniacal liquor with make fertilizer.
Embodiment two:
Get L-glutamic acid and cultivate sophisticated fermented liquid, iso-electric point gets the mycetome fermented liquid of extracting section L-glutamic acid after extracting L-glutamic acid, its L-glutamic acid content is/100 milliliters of 1.5 grams, PH3, get 20 tons of liquid and enter concentratedly, use reverse-flow triple-effect evaporator, 50 ℃ of the temperature of triple effect, vacuum tightness 0.1Mpa, 70 ℃ of second temperature of imitating, vacuum tightness 0.08Mpa, 85 ℃ of first temperature of imitating, vacuum tightness 0.06Mpa, concentration ratio gets 6.6 tons of concentrated solutions at 3: 1, and its amount of glutamic acid is/100 milliliters of 4.5 grams, drops into 150 kilograms in L-glutamic acid, purity 90%, make by dense filtrate body L-glutamic acid content reach 6.6 the gram/100 milliliters, concentrated solution is heated to 60 ℃, through pressure filter filter clear liquid, clear liquid is lowered the temperature through iso-electric point, freezing, extract 350 kilograms in L-glutamic acid, purity 88%, its secondary crystal mother liquor L-glutamic acid content is/100 milliliters of 1.8 grams, behind this mother liquor reconcentration with in the ammoniacal liquor and make fertilizer, the filtrate of mycetome is capable of using as feed.
As can be seen, the remarkable technical progress of the inventive method, it is fairly obvious comparing with former processing method.
Claims (2)
1, cultivate the method for extracting L-glutamic acid the sophisticated fermented liquid from L-glutamic acid, comprise fermented liquid iso-electric point extraction L-glutamic acid, choosing is fit to concentration ratio, the condensing crystal mother liquor, filtering and concentrating liquid, iso-electric point is extracted L-glutamic acid from concentrated solution, the fermented liquid that it is characterized in that the L-glutamic acid of extracting section of concentrated mycetome, adjustment is concentrated the L-glutamic acid content of liquid/100 milliliters of 1.7～2.8 grams, or spissated liquid, L-glutamic acid content is adjusted at/100 milliliters of 4.5～8.5 grams, and concentration evaporator is an one-pass type flow evaporation device, 50 ℃～85 ℃ of temperature, vacuum tightness 0.06Mpa～0.1Mpa drops into defoamer, and (the bubble enemy is no more than 0.04%, or methyl-silicone oil is no more than 0.04%, or vegetables oil is no more than 0.4%) in any.
2, require described method according to power 1, it is characterized in that one-pass type flow evaporation device is triple effect rising-film evaporator (or falling-film evaporator).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92112240 CN1085954A (en) | 1992-10-16 | 1992-10-16 | The method of extraction of glutamic acid from concentrated bacteria-contained fermentation liquor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92112240 CN1085954A (en) | 1992-10-16 | 1992-10-16 | The method of extraction of glutamic acid from concentrated bacteria-contained fermentation liquor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1085954A true CN1085954A (en) | 1994-04-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92112240 Pending CN1085954A (en) | 1992-10-16 | 1992-10-16 | The method of extraction of glutamic acid from concentrated bacteria-contained fermentation liquor |
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| Country | Link |
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| CN (1) | CN1085954A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7198943B2 (en) | 2001-02-20 | 2007-04-03 | Ajinomoto Co., Inc. | Organic nitrogen-containing composition and fertilizer comprising the same |
| CN102285895A (en) * | 2011-08-30 | 2011-12-21 | 无锡荣丰生物工程有限公司 | Triple-effect internal circulation continuous evaporation crystallization process of phenylalanine |
-
1992
- 1992-10-16 CN CN 92112240 patent/CN1085954A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7198943B2 (en) | 2001-02-20 | 2007-04-03 | Ajinomoto Co., Inc. | Organic nitrogen-containing composition and fertilizer comprising the same |
| CN1318599C (en) * | 2001-02-20 | 2007-05-30 | 味之素株式会社 | Composition containing organic nitrogen and its fertilizer containing said composition |
| CN102285895A (en) * | 2011-08-30 | 2011-12-21 | 无锡荣丰生物工程有限公司 | Triple-effect internal circulation continuous evaporation crystallization process of phenylalanine |
| CN102285895B (en) * | 2011-08-30 | 2013-08-07 | 无锡荣丰生物工程有限公司 | Triple-effect internal circulation continuous evaporation crystallization process of phenylalanine |
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