CN108593899A - It is a kind of to utilize chick chorioallantoic membrane model inspection mask substrate and the irritating method of medical dressing - Google Patents
It is a kind of to utilize chick chorioallantoic membrane model inspection mask substrate and the irritating method of medical dressing Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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Abstract
Chick chorioallantoic membrane model inspection mask substrate and the irritating method of medical dressing are utilized the invention discloses a kind of, belong to Membranes irritation technical field, this method is the first blood vessel state data obtained in each fertilization chick chorioallantoic membrane detection zone；Obtain each sample is spread on correspondingly in detection zone act on a period of time after and remove the second blood vessel state data in the detection zone of sample；Two groups of data are respectively compared, vascular reaction degree result is obtained；It is calculated according to result and obtains single vessel extent of reaction mean value；According to the stimulation levels for being worth to sample.The present invention is suitable for evaluating the irritation of unlike material Membranes and classification, it is kind of a simple, economic, highly sensitive detection method, can effectively replace human body patch test and consumer sensory's test, instruct enterprise screen and the mask substrate of developing suitable special crowd (sensitive skin crowd, skin lesion crowd, infant) etc. directly with the Membranes of skin contact.
Description
Technical field
The present invention relates to Membranes irritation technical fields, and in particular to a kind of to be examined using chick chorioallantoic membrane model
This irritating method of a kind of insoluble thin-film material of solid of survey mask substrate and medical dressing etc..
Background technology
With the continuous improvement of economic development and national life level, demand of the people to cosmetology constantly increases.Face
Film becomes the favorite skin care item of many people seeking beauties because of its advantage such as easy to carry and with obvious effects.Facial mask is according to its carrier
Form can be divided into face paste formula and non-face is mounted, and face paste formula mask substrate is mainly non-woven fabrics, tencel, silk, bamboo charcoal fiber etc., and
The mounted facial mask of non-face is mainly soluble formulation at membrane material.In recent years since, China's facial mask market development is rapid.Currently on the market
Facial mask brand number have reached 300 or more, market scale is just increased with every year about 30% speed.China uses facial mask
Crowd reaches 45%, has much surmounted South Korea and Taiwan.However in booming market, there is also secret worries behind.
For the consumer of partial skin sensitivity after application mask product, there are the adverse reactions such as itch, shouting pain and redness in face.Facial mask produces
The safety issue of product increasingly obtains the concern of consumer and manufacturing enterprise.
It is primarily directed to this kind of water such as film liquid/oil-soluble sample for the safety research of facial mask both at home and abroad at present,
Research is with reference to both at home and abroad for the detection of the irritation of water/oil-soluble sample and grading evaluation criteria.Due to common external thin
It is water solubility that born of the same parents and tissue model, which generally require sample, so being not suitable for water-insoluble membrane material.For example, utilizing chicken embryo chorion
The allantois membrane structure feature similar to people's conjunctiva, European Union and the U.S. use it for Eye irritation object or the mirror of erosion in chemicals
It is fixed.The standard method promulgated has chicken embryo chorioallantoic membrane blood vessel to test (Chorioallantoic membrane
Vascular assay, CAMVA) and chicken embryo chorioallantois film test (Hen`s egg test on the chorio-
Allantoic membrane, HET-CAM).However both methods is only applicable to liquid and water/oil-soluble sample, Bu Nengyong
It is evaluated in the irritation of solid membrane material.
For example, the detection method of a kind of cosmetics and its raw material is disclosed in Chinese patent CN105158449A, master
The step is wanted to include：Fertilization chicken embryo is taken, chick chorioallantoic membrane is prepared；Ptfe ring is placed in chorioallantoic membrane；By sample to be tested
It is added to tested region in ring；Fertilization chicken embryo is placed in incubator after being incubated a period of time and is taken out, compare in ring with the outer villus of ring
Vascular change situation on chorioallantoic membrane is given according to the extent of reaction of blood vessel and scores, and the stimulation of sample to be tested is determined according to scoring
Grade.The detection method is also to be directed to this kind of water of cosmetics/oil-soluble sample, copes with sample at the trial and is first diluted, no
It can be suitably used for the irritating detection and analysis of the insoluble thin-film material of solid.
Therefore, for the irritation of mask substrate or the insoluble thin-film material of this kind of solid of medical dressing itself, at present still
Lack ripe effective irritation determination method.
Invention content
Therefore, technical problems to be solved of the embodiment of the present invention are in the prior art for water/oil-soluble sample
Irritation determination method is not suitable for the insoluble thin-film material of solid.
For this purpose, a kind of solid of the embodiment of the present invention it is non-aqueous/the irritating detection method of oil-soluble Membranes, including with
Lower step：
Obtain the first blood vessel state data in each fertilization chick chorioallantoic membrane detection zone；
Obtain by each solid it is non-aqueous/samples of oil-soluble Membranes spreads in the detection zone and makees correspondingly
With after a period of time and removing the second blood vessel state data in the detection zone of the sample；
First blood vessel state data and the second blood vessel state data described in being respectively compared every group obtain each detection zone
Vascular reaction degree result in domain；
It is calculated according to the vascular reaction degree result in the detection zone, it is equal to obtain the single vessel extent of reaction
Value；
The stimulation levels of the acquisition sample are judged according to the single vessel extent of reaction mean value.
Preferably, it is described be respectively compared every group described in the first blood vessel state data and the second blood vessel state data, obtain every
The step of vascular reaction degree result in a detection zone includes：
Judge that the second blood vessel state data are compared with the variation degree of the first blood vessel state data described in every group respectively；
If blood vessel state is reactionless, the vascular reaction degree result that effect value is equal to 0 is obtained；
It is not flowed if blood vessel state is blood vessel, obtains the vascular reaction degree result that effect value is equal to 1；
If congested value range is more than 0 and is less than or equal to first threshold, the vascular reaction degree that effect value is equal to 2 is obtained
As a result；
If congested value range is more than first threshold and is less than or equal to second threshold, the blood vessel that effect value is equal to 3 is obtained
Extent of reaction result；
If congested value range is more than second threshold and is less than or equal to third threshold value, the blood vessel that effect value is equal to 4 is obtained
Extent of reaction result；
If congested value range is more than third threshold value, the vascular reaction degree result that effect value is equal to 5 is obtained；
If bleeding value range is more than 0 and is less than or equal to the 4th threshold value, the vascular reaction degree that effect value is equal to 6 is obtained
As a result；
If bleeding value range is more than the 4th threshold value, the vascular reaction degree result that effect value is equal to 7 is obtained.
Preferably, the step of the stimulation levels for judging to obtain the sample according to the single vessel extent of reaction mean value
Suddenly include：
Vascular stimulation index is obtained according to the single vessel extent of reaction mean value and single vessel reaction Incidence calculus；
The vascular stimulation index is compared calculating with the vascular stimulation index of negative control, correction stimulation is obtained and refers to
Number；
Judge whether positive reaction incidence is less than or equal to probability threshold value, the positive reaction is effect value more than 0
Vascular reaction degree result；
When positive reaction incidence is less than or equal to probability threshold value, judge whether the correction stimulus index is less than 2；
When correcting stimulus index less than 2, the stimulation levels for obtaining the sample are nonirritant.
Preferably, the step of the stimulation levels for judging to obtain the sample according to the single vessel extent of reaction mean value
Suddenly further include：
When positive reaction incidence be more than probability threshold value when, the correction stimulus index respectively with the first stimulus threshold
Size between value, the second stimulus threshold, third stimulus threshold and the 4th stimulus threshold；
If the correction stimulus index is more than 0 and is less than or equal to the first stimulus threshold, the stimulation of the sample is obtained
Grade is pole slight stimulation；
If the correction stimulus index is more than the first stimulus threshold and is less than or equal to the second stimulus threshold, described in acquisition
The stimulation levels of sample are slight stimulation；
If the correction stimulus index is more than the second stimulus threshold and is less than or equal to third stimulus threshold, described in acquisition
The stimulation levels of sample are moderate irritation；
If the correction stimulus index is more than third stimulus threshold and is less than or equal to the 4th stimulus threshold, described in acquisition
The stimulation levels of sample are strong and stimulating.
Preferably, the solid it is non-aqueous/oil-soluble Membranes include mask substrate, face paper, film cloth, face paste formula face
Film, membrane material and medical dressing.
A kind of solid of the embodiment of the present invention is non-aqueous/the irritating detection device of oil-soluble Membranes, including：
First acquisition unit, for obtaining the first blood vessel state number in each fertilization chick chorioallantoic membrane detection zone
According to；
Second acquisition unit, for obtain by each solid it is non-aqueous/samples of oil-soluble Membranes applies correspondingly
After acting on a period of time in the detection zone and remove the second blood vessel state in the detection zone of the sample
Data；
First compare with vascular reaction degree result acquiring unit, for the first blood vessel state number described in being respectively compared every group
According to the second blood vessel state data, obtain the vascular reaction degree result in each detection zone；
First computing unit obtains single for being calculated according to the vascular reaction degree result in the detection zone
One vascular reaction degree mean value；
Stimulation levels acquiring unit, the thorn for judging the acquisition sample according to the single vessel extent of reaction mean value
Swash grade.
Preferably, described first compares and includes with vascular reaction degree result acquiring unit：
First judging unit, for judging that the second blood vessel state data are compared with the first blood vessel state data described in every group respectively
Variation degree；
First vascular reaction degree result acquiring unit obtains effect value and is equal to 0 if reactionless for blood vessel state
Vascular reaction degree result；
Second vascular reaction degree result acquiring unit is imitated if being that blood vessel does not flow for blood vessel state
The vascular reaction degree result equal to 1 should be worth；
Third vascular reaction degree result acquiring unit, if being more than 0 for congested value range and being less than or equal to the first threshold
Value then obtains the vascular reaction degree result that effect value is equal to 2；
4th vascular reaction degree result acquiring unit, if being more than first threshold for congested value range and being less than or equal to
Second threshold then obtains the vascular reaction degree result that effect value is equal to 3；
5th vascular reaction degree result acquiring unit, if being more than second threshold for congested value range and being less than or equal to
Third threshold value then obtains the vascular reaction degree result that effect value is equal to 4；
6th vascular reaction degree result acquiring unit obtains effect if being more than third threshold value for congested value range
Vascular reaction degree result of the value equal to 5；
7th vascular reaction degree result acquiring unit, if being more than 0 for bleeding value range and being less than or equal to the 4th threshold
Value then obtains the vascular reaction degree result that effect value is equal to 6；
8th vascular reaction degree result acquiring unit obtains effect if being more than the 4th threshold value for bleeding value range
Vascular reaction degree result of the value equal to 7.
Preferably, the stimulation levels acquiring unit includes：
Second computing unit, for reacting Incidence calculus according to the single vessel extent of reaction mean value and single vessel
Obtain vascular stimulation index；
Third computing unit, based on being compared the vascular stimulation index with the vascular stimulation index of negative control
It calculates, obtains correction stimulus index；
Second judgment unit, it is described positive anti-for judging whether positive reaction incidence is less than or equal to probability threshold value
It should be the vascular reaction degree result that effect value is more than 0；
Third judging unit, for when positive reaction incidence is less than or equal to probability threshold value, judging the correction thorn
Whether sharp index is less than 2；
The first estate acquiring unit, the stimulation levels for when correcting stimulus index less than 2, obtaining the sample are nothing
Irritation.
Preferably, the stimulation levels acquiring unit further includes：
Comparing unit, for when positive reaction incidence is more than probability threshold value, the correction stimulus index to be distinguished
With the size between the first stimulus threshold, the second stimulus threshold, third stimulus threshold and the 4th stimulus threshold；
Second grade acquiring unit, if being more than 0 for the correction stimulus index and being less than or equal to the first stimulus threshold,
The stimulation levels for then obtaining the sample are pole slight stimulation；
Tertiary gradient acquiring unit, if being more than the first stimulus threshold for the correction stimulus index and being less than or equal to the
Two stimulus thresholds, the then stimulation levels for obtaining the sample are slight stimulation；
Fourth estate acquiring unit, if being more than the second stimulus threshold for the correction stimulus index and being less than or equal to the
Tristimulus threshold value, the then stimulation levels for obtaining the sample are moderate irritation；
5th grade acquiring unit, if being more than third stimulus threshold for the correction stimulus index and being less than or equal to the
Four stimulus thresholds, the then stimulation levels for obtaining the sample are strong and stimulating.
A kind of solid of the embodiment of the present invention is non-aqueous/the irritating detecting system of oil-soluble Membranes, including above-mentioned inspection
Survey device and observation recorder；
The observation recorder is connect with the detection device, for the image in acquisition testing region and is transferred to described
Detection device.
The technical solution of the embodiment of the present invention, has the following advantages that：
Solid provided in an embodiment of the present invention is non-aqueous/the irritating detection method of oil-soluble Membranes, apparatus and system,
By obtaining the blood vessel state data of same detection zone before and after applying sample, without the operations such as being diluted to sample, from
And it is applicable to the irritation detection of the insoluble thin-film material of solid, it overcomes and is directed to water/oil-soluble sample in the prior art
The not applicable defect of irritation determination method.And carry out difference by the data front and back to sample application in the same area
Quantitative comparison, compared to the comparative approach in sample-adding ring and outside ring, test environment is more with uniformity, the detection knot obtained
Fruit is more comparable, is more accurate, improves accuracy and the precision of detection, to be consumer and facial mask research staff
When membrane material is selected with improveing, a kind of simpler, efficient, highly sensitive in-vitro screening and evaluation method are provided.
Description of the drawings
It, below will be in specific implementation mode in order to illustrate more clearly of the technical solution in the specific embodiment of the invention
Required attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is some embodiment party of the present invention
Formula without creative efforts, can also obtain according to these attached drawings for those of ordinary skill in the art
Obtain other attached drawings.
Fig. 1 be in the embodiment of the present invention 1 solid it is non-aqueous/one of the irritating detection method of oil-soluble Membranes it is specific
Exemplary flow chart；
Fig. 2 be in the embodiment of the present invention 2 solid it is non-aqueous/one of the irritating detection device of oil-soluble Membranes it is specific
Exemplary functional block diagram.
Specific implementation mode
Technical scheme of the present invention is clearly and completely described below in conjunction with attached drawing, it is clear that described implementation
Example is a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, ordinary skill
The every other embodiment that personnel are obtained without making creative work, shall fall within the protection scope of the present invention.
In the description of the present invention, it should be noted that term " first ", " second ", " third " etc. are only used for description mesh
, it is not understood to indicate or imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " connection " should be done
It broadly understood, for example, it may be mechanical connection, can also be electrical connection；It can be directly connected, intermediary can also be passed through
It is indirectly connected, can also be the connection inside two elements；Can be wirelessly connected, can also be wired connection.For ability
For the those of ordinary skill in domain, the concrete meaning of above-mentioned term in the present invention can be understood with concrete condition.
As long as in addition, technical characteristic involved in invention described below different embodiments non-structure each other
It can be combined with each other at conflict.
Embodiment 1
The present embodiment provides a kind of solid it is non-aqueous/the irritating detection method of oil-soluble Membranes, solid is non-aqueous/oil is molten
Property Membranes can also be such as can be mask substrate, face paper, film cloth, face paste formula facial mask, membrane material, medical dressing
The Membranes etc. that disinfected paper napkin, female sanitary towel and baby' diaper etc. are directly contacted with sensitive skin.As shown in Figure 1, the inspection
Survey method includes the following steps：
S1, each the first blood vessel state data being fertilized in chick chorioallantoic membrane detection zone are obtained.Fertilization chicken embryo example
Such as can be SPF grades or non-SPF grades of fertilization chicken embryo.
S2, obtain by each solid it is non-aqueous/samples of oil-soluble Membranes spreads in detection zone make correspondingly
With after a period of time and removing the second blood vessel state data in the detection zone of sample.Preferably, it is added in detection zone
After the samples such as membrane material or dressing, sterile water need to be added to be soaked, the purpose is to which sample and chorioallantoic membrane can be made better connect
It touches.Detection zone can arbitrarily be divided according to sample shape, such as sample can be the round membrane material that sterilized diameter is about 6mm,
Detection zone can use polytetrafluoroethylene (PTFE) "O" ring to draw a circle to approve, and sample is placed in "O" ring by when detection.Above-mentioned effect is for a period of time
It can be selected according to the difference of sample, generally be incubated 15min or so.
S3, every group of first blood vessel state data and the second blood vessel state data are respectively compared, obtained in each detection zone
Vascular reaction degree result.
S4, it is calculated according to the vascular reaction degree result in detection zone, obtains single vessel extent of reaction mean value.
S5, judged to obtain the stimulation levels of sample according to single vessel extent of reaction mean value.
Above-mentioned solid is non-aqueous/the irritating detection method of oil-soluble Membranes, it is same by obtaining by step S1-S5
Blood vessel state data of one detection zone before and after applying sample, without the operations such as being diluted to sample, so as to be suitable for
The irritation of the insoluble thin-film material of solid detects, and overcomes the irritation detection for being directed to water/oil-soluble sample in the prior art
The not applicable defect of analysis method.And the quantitative comparison of difference is carried out by the data front and back to sample application in the same area,
Compared to the comparative approach in sample-adding ring and outside ring, test environment is more with uniformity, and the testing result obtained more has
There is comparativity, more accurate, accuracy and the precision of detection is improved, to be selected in membrane material for consumer and facial mask research staff
When selecting with improvement, a kind of simpler, efficient, highly sensitive in-vitro screening and evaluation method are provided.
Preferably, above-mentioned steps S3 is respectively compared every group of first blood vessel state data and the second blood vessel state data, obtains
Vascular reaction degree result in each detection zone the step of include：
S31, variation degree of the every group of second blood vessel state data compared with the first blood vessel state data is judged respectively；
If S32, blood vessel state are reactionless, the vascular reaction degree result that effect value is equal to 0 is obtained；
It is not flowed if S33, blood vessel state are blood vessel, obtains the vascular reaction degree result that effect value is equal to 1；
If S34, congested value range are more than 0 and are less than or equal to first threshold, it is anti-equal to 2 blood vessel to obtain effect value
Answer degree result；
If S35, congested value range are more than first threshold and are less than or equal to second threshold, obtains effect value and be equal to 3
Vascular reaction degree result；
If S36, congested value range are more than second threshold and are less than or equal to third threshold value, obtains effect value and be equal to 4
Vascular reaction degree result；
If S37, congested value range are more than third threshold value, the vascular reaction degree result that effect value is equal to 5 is obtained；
If S38, bleeding value range are more than 0 and are less than or equal to the 4th threshold value, it is anti-equal to 6 blood vessel to obtain effect value
Answer degree result；
If S39, bleeding value range are more than the 4th threshold value, the vascular reaction degree result that effect value is equal to 7 is obtained.
Wherein, the selection of first threshold, second threshold, third threshold value, the 4th threshold value can come according to different detection objects etc.
It determines, if first threshold can be 25% annular detection zone, second threshold can be 50% annular detection zone, third threshold
Value can be 75% annular detection zone, and the 4th threshold value can be 50% annular detection zone, as shown in table 1 below.
1 vascular reaction degree effect value of table corresponds to table
S31-S39 through the above steps, by comparing the variation journey of the front and back acquisition blood vessel state of same detection zone sample-adding
It, is divided into eight grades, realizes the algorithm automatic decision of vascular reaction degree, to improve obtained effect by degree in detail
The precision of value and accuracy improve the objectivity of subsequent stimuli judgement.
Preferably, above-mentioned steps S4 is calculated according to the vascular reaction degree result in inspection area, is obtained single
The step of vascular reaction degree mean value includes：
Single vessel extent of reaction mean value is calculated, calculation formula is as follows：
Wherein, Si is the sum of N groups i-th kind of effect value of parallel middle generation of experiment, that is, the effect of i-th kind of vascular reaction occurs
The sum of value；I can be with value：I=1 expression ghost blood vessels, i=2 expressions hyperemia (including slight, moderate, severe, pole severe are filled
Blood), i=3 indicates bleeding (including slight, severe bleeding).The calculating of the formula is applicable not only to sample to be tested experiment, is also applicable in
In using sterile water as the experiment of negative control (NC), and with experiment that ethyl alcohol (purity ＞ 99.7%) is positive control (PC).
For the present embodiment using sterile water as negative control (NC), ethyl alcohol (purity ＞ 99.7%) is positive control (PC).
Ni is the generation quantity of i-th kind of vascular reaction (value of i is corresponding with the value of i in Si) during N groups are parallel.
Preferably, the step for judging to obtain the stimulation levels of sample according to single vessel extent of reaction mean value of above-mentioned steps S5
Suddenly include：
S51, vascular stimulation index is obtained according to single vessel extent of reaction mean value and single vessel reaction Incidence calculus.
It is as follows：
It calculates single vessel and reacts incidence, calculation formula is as follows：
Vascular stimulation index is obtained according to single vessel extent of reaction mean value and single vessel reaction Incidence calculus, is calculated
Formula is as follows：
The calculating of the formula is applicable not only to sample to be tested experiment, applies also for using sterile water as the examination of negative control (NC)
Test, and with ethyl alcohol (purity ＞ 99.7%) be positive control (PC) experiment.Note sample sets vascular stimulation index is Isp, negative
Control group vascular stimulation index is Inc。
S52, vascular stimulation index with the vascular stimulation index of negative control is compared to calculating, obtains correction stimulation and refers to
Number (CI), calculation formula is as follows：
CI=Isp-Inc
S53, judge whether positive reaction incidence is less than or equal to probability threshold value, positive reaction is effect value more than 0
Vascular reaction degree is as a result, as ghost blood vessel, capillary injection and the bleeding in upper table can be considered positive reaction.It is positive
Reaction incidence is the sum of single vessel reaction incidence, general parallel test time in the multiple parallel test for a sample
Number can be parallel 6-8 times, and calculation formula is：
When positive reaction incidence is less than or equal to probability threshold value, S54 is entered step；When positive reaction incidence is more than
When probability threshold value, S56 is entered step.Probability threshold value can be chosen according to different sampling test characteristics, such as can be 50% or so.
S54, judge to correct whether stimulus index is less than 2；When correcting stimulus index less than 2, S55 is entered step.
S55, the stimulation levels for obtaining sample are nonirritant.
S56, compare correction stimulus index respectively with the first stimulus threshold, the second stimulus threshold, third stimulus threshold and the
Size between four stimulus thresholds；
If S57, correction stimulus index are more than 0 and are less than or equal to the first stimulus threshold, the stimulation levels of sample are obtained
For pole slight stimulation；If correcting stimulus index to be more than the first stimulus threshold and be less than or equal to the second stimulus threshold, obtain
The stimulation levels of sample are slight stimulation；If correcting stimulus index to be more than the second stimulus threshold and stimulate less than or equal to third
Threshold value, the then stimulation levels for obtaining sample are moderate irritation；If correction stimulus index be more than third stimulus threshold and be less than or
Equal to the 4th stimulus threshold, then the stimulation levels for obtaining sample are strong and stimulating.
Wherein, the first stimulus threshold, the second stimulus threshold, third stimulus threshold and the 4th stimulus threshold can be according to experiments pair
The difference of elephant is chosen, and such as the first stimulus threshold can be 2, and the second stimulus threshold can be 4, and third stimulus threshold can be the 6, the 4th
Stimulus threshold can be 8.
S51-S58 through the above steps, by according to single vessel extent of reaction mean value, with reference to test of many times as a result, with
Correction stimulus index is index, determines irritation grade so that result is more accurate, has more actual reference.
Below as the present embodiment detection method respectively to pure cotton mask substrate, fruit fibre mask substrate, tencel facial mask base
Cloth, hospital gauze, PET superfine fibre films are detected, and testing result is as follows.
Example 1
Pure cotton mask substrate sample done 6 groups it is parallel, it is as a result as follows：
Blood vessel differential responses percentage and positive incidence are：
As it can be seen that the positive reaction incidence X (%) of pure cotton mask substrate is 33.3, and the CI values of its caused vascular reaction
It is 0.48, is less than 2, therefore it is nonirritant testing result to obtain pure cotton mask substrate.
Example 2
Fruit fibre mask substrate sample done 6 groups it is parallel, it is as a result as follows：
Blood vessel differential responses percentage and positive incidence are：
As it can be seen that the positive reaction incidence X (%) of fruit fibre mask substrate is 100, and the CI values of its caused vascular reaction
It is 1.27, is less than 2, therefore obtains the testing result that fruit fibre mask substrate is pole slight stimulation.
Example 3
Tencel mask substrate sample done 6 groups it is parallel, it is as a result as follows：
Blood vessel differential responses percentage and positive incidence are：
As it can be seen that the positive reaction incidence X (%) of tencel fibre mask substrate is 100, and the CI of its caused vascular reaction
Value is 2.17, therefore obtains the testing result that tencel mask substrate is slight stimulation.
Example 4
Hospital gauze sample done 6 groups it is parallel, it is as a result as follows：
Blood vessel differential responses percentage and positive incidence
As it can be seen that the positive reaction incidence X (%) of hospital gauze is 116.6, and the CI values of its caused vascular reaction are
5.67, therefore it is the irritating testing result of moderate to obtain hospital gauze.
Example 5
PET superfine fibre membrane samples done 6 groups it is parallel, it is as a result as follows：
Blood vessel differential responses percentage and positive incidence are：
As it can be seen that the positive reaction incidence X (%) of PET superfine fibre films is 150, and the CI values of its caused vascular reaction
It is 5.45, therefore it is the irritating testing result of moderate to obtain PET superfine fibre films.
Embodiment 2
Corresponding to embodiment 1 solid it is non-aqueous/the irritating detection method of oil-soluble Membranes, as shown in Fig. 2, this
Embodiment provide a kind of solid it is non-aqueous/the irritating detection device of oil-soluble Membranes, including：
First acquisition unit 1, for obtaining the first blood vessel state in each fertilization chick chorioallantoic membrane detection zone
Data；
Second acquisition unit 2, for obtain by each solid it is non-aqueous/samples of oil-soluble Membranes applies correspondingly
After acting on a period of time in detection zone and remove the second blood vessel state data in the detection zone of sample；
First compare with vascular reaction degree result acquiring unit 3, for being respectively compared every group of first blood vessel state data
With the second blood vessel state data, the vascular reaction degree result in each detection zone is obtained；
First computing unit 4 obtains single blood for being calculated according to the vascular reaction degree result in detection zone
Tube reaction degree mean value；
Stimulation levels acquiring unit 5, the stimulation levels for judging acquisition sample according to single vessel extent of reaction mean value.
Solid provided in this embodiment is non-aqueous/the irritating detection device of oil-soluble Membranes, pass through and obtains same inspection
Blood vessel state data of the region before and after applying sample are surveyed, without the operations such as being diluted to sample, so as to be suitable for solid
The irritation of insoluble thin-film material detects, and overcomes the irritation detection and analysis for being directed to water/oil-soluble sample in the prior art
The not applicable defect of method.And the quantitative comparison of difference is carried out by the data front and back to sample application in the same area, it compares
In the comparative approach in sample-adding ring and outside ring, test environment is more with uniformity, and the testing result obtained more has can
Than property, it is more accurate, improve accuracy and the precision of detection, to for consumer and facial mask research staff membrane material selection with
When improvement, a kind of simpler, efficient, highly sensitive in-vitro screening and evaluation method are provided.
Preferably, first compares and includes with vascular reaction degree result acquiring unit：
First judging unit, for judging variation of the every group of second blood vessel state data compared with the first blood vessel state data respectively
Degree；
First vascular reaction degree result acquiring unit obtains effect value and is equal to 0 if reactionless for blood vessel state
Vascular reaction degree result；
Second vascular reaction degree result acquiring unit is imitated if being that blood vessel does not flow for blood vessel state
The vascular reaction degree result equal to 1 should be worth；
Third vascular reaction degree result acquiring unit, if being more than 0 for congested value range and being less than or equal to the first threshold
Value then obtains the vascular reaction degree result that effect value is equal to 2；
4th vascular reaction degree result acquiring unit, if being more than first threshold for congested value range and being less than or equal to
Second threshold then obtains the vascular reaction degree result that effect value is equal to 3；
5th vascular reaction degree result acquiring unit, if being more than second threshold for congested value range and being less than or equal to
Third threshold value then obtains the vascular reaction degree result that effect value is equal to 4；
6th vascular reaction degree result acquiring unit obtains effect if being more than third threshold value for congested value range
Vascular reaction degree result of the value equal to 5；
7th vascular reaction degree result acquiring unit, if being more than 0 for bleeding value range and being less than or equal to the 4th threshold
Value then obtains the vascular reaction degree result that effect value is equal to 6；
8th vascular reaction degree result acquiring unit obtains effect if being more than the 4th threshold value for bleeding value range
Vascular reaction degree result of the value equal to 7.
Preferably, stimulation levels acquiring unit includes：
Second computing unit, for being obtained according to single vessel extent of reaction mean value and single vessel reaction Incidence calculus
Vascular stimulation index；
Third computing unit, for vascular stimulation index to be compared calculating with the vascular stimulation index of negative control,
Obtain correction stimulus index；
Second judgment unit, for judging whether positive reaction incidence is less than or equal to probability threshold value, positive reaction is
Effect value is more than 0 vascular reaction degree result；
Third judging unit, for when positive reaction incidence is less than or equal to probability threshold value, judging that correction stimulation refers to
Whether number is less than 2；
The first estate acquiring unit, the stimulation levels for when correcting stimulus index less than 2, obtaining sample are non-stimulated
Property.
Preferably, stimulation levels acquiring unit further includes：
Comparing unit, for when positive reaction incidence is more than probability threshold value, comparing correction stimulus index respectively with the
Size between one stimulus threshold, the second stimulus threshold, third stimulus threshold and the 4th stimulus threshold；
If second grade acquiring unit obtains for correcting stimulus index more than 0 and being less than or equal to the first stimulus threshold
The stimulation levels for obtaining sample are pole slight stimulation；
Tertiary gradient acquiring unit, if being more than the first stimulus threshold and less than or equal to the second thorn for correcting stimulus index
Swash threshold value, then the stimulation levels for obtaining sample are slight stimulation；
Fourth estate acquiring unit, if being more than the second stimulus threshold and less than or equal to third thorn for correcting stimulus index
Swash threshold value, then the stimulation levels for obtaining sample are moderate irritation；
5th grade acquiring unit, if being more than third stimulus threshold and less than or equal to the 4th thorn for correcting stimulus index
Swash threshold value, then the stimulation levels for obtaining sample are strong and stimulating.
Embodiment 3
The present embodiment provides a kind of solid it is non-aqueous/oil-soluble Membranes irritating detecting systems, including embodiment 2
Detection device and observation recorder；Observation recorder can be the image collecting devices such as stereoscopic disecting microscope, camera.
Observation recorder is connect with detection device, for the image in acquisition testing region and is transferred to detection device.It is excellent
Selection of land, observation recorder include Image Acquisition window and cylindrical light source, which is located at Image Acquisition window
Lower section, close to checking matter, the illumination region of cylindrical light source is uniformly distributed on entire cylinder inner wall, to be carried for checking matter
For the illumination of uniform, enough brightness, improve the clarity and resolution ratio of Image Acquisition, so can improve detection accuracy and
Precision.
It should be understood by those skilled in the art that, the embodiment of the present invention can be provided as method, system or computer program
Product.Therefore, complete hardware embodiment, complete software embodiment or reality combining software and hardware aspects can be used in the present invention
Apply the form of example.Moreover, the present invention can be used in one or more wherein include computer usable program code computer
The computer program production implemented in usable storage medium (including but not limited to magnetic disk storage, CD-ROM, optical memory etc.)
The form of product.
The present invention be with reference to according to the method for the embodiment of the present invention, the flow of equipment (system) and computer program product
Figure and/or block diagram describe.It should be understood that can be realized by computer program instructions every first-class in flowchart and/or the block diagram
The combination of flow and/or box in journey and/or box and flowchart and/or the block diagram.These computer programs can be provided
Instruct the processor of all-purpose computer, special purpose computer, Embedded Processor or other programmable data processing devices to produce
A raw machine so that the instruction executed by computer or the processor of other programmable data processing devices is generated for real
The device for the function of being specified in present one flow of flow chart or one box of multiple flows and/or block diagram or multiple boxes.
These computer program instructions, which may also be stored in, can guide computer or other programmable data processing devices with spy
Determine in the computer-readable memory that mode works so that instruction generation stored in the computer readable memory includes referring to
Enable the manufacture of device, the command device realize in one flow of flow chart or multiple flows and/or one box of block diagram or
The function of being specified in multiple boxes.
These computer program instructions also can be loaded onto a computer or other programmable data processing device so that count
Series of operation steps are executed on calculation machine or other programmable devices to generate computer implemented processing, in computer or
The instruction executed on other programmable devices is provided for realizing in one flow of flow chart or multiple flows and/or block diagram one
The step of function of being specified in a box or multiple boxes.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes still within the protection scope of the invention.
Claims (10)
1. a kind of solid is non-aqueous/the irritating detection method of oil-soluble Membranes, which is characterized in that include the following steps：
Obtain the first blood vessel state data in each fertilization chick chorioallantoic membrane detection zone；
Obtain by each solid it is non-aqueous/samples of oil-soluble Membranes spreads in the detection zone and acts on one correspondingly
After the section time and remove the second blood vessel state data in the detection zone of the sample；
First blood vessel state data and the second blood vessel state data described in being respectively compared every group obtain in each detection zone
Vascular reaction degree result；
It is calculated according to the vascular reaction degree result in the detection zone, obtains single vessel extent of reaction mean value；
The stimulation levels of the acquisition sample are judged according to the single vessel extent of reaction mean value.
2. detection method according to claim 1, which is characterized in that it is described be respectively compared every group described in the first blood vessel state
Data and the second blood vessel state data, the step of obtaining the vascular reaction degree result in each detection zone include：
Judge that the second blood vessel state data are compared with the variation degree of the first blood vessel state data described in every group respectively；
If blood vessel state is reactionless, the vascular reaction degree result that effect value is equal to 0 is obtained；
It is not flowed if blood vessel state is blood vessel, obtains the vascular reaction degree result that effect value is equal to 1；
If congested value range is more than 0 and is less than or equal to first threshold, the vascular reaction degree knot that effect value is equal to 2 is obtained
Fruit；
If congested value range is more than first threshold and is less than or equal to second threshold, the vascular reaction that effect value is equal to 3 is obtained
Degree result；
If congested value range is more than second threshold and is less than or equal to third threshold value, the vascular reaction that effect value is equal to 4 is obtained
Degree result；
If congested value range is more than third threshold value, the vascular reaction degree result that effect value is equal to 5 is obtained；
If bleeding value range is more than 0 and is less than or equal to the 4th threshold value, the vascular reaction degree knot that effect value is equal to 6 is obtained
Fruit；
If bleeding value range is more than the 4th threshold value, the vascular reaction degree result that effect value is equal to 7 is obtained.
3. detection method according to claim 2, which is characterized in that described according to the single vessel extent of reaction mean value
Judge that the step of obtaining the stimulation levels of the sample includes：
Vascular stimulation index is obtained according to the single vessel extent of reaction mean value and single vessel reaction Incidence calculus；
The vascular stimulation index is compared calculating with the vascular stimulation index of negative control, obtains correction stimulus index；
Judge whether positive reaction incidence is less than or equal to probability threshold value, the positive reaction is the blood vessel that effect value is more than 0
Extent of reaction result；
When positive reaction incidence is less than or equal to probability threshold value, judge whether the correction stimulus index is less than 2；
When correcting stimulus index less than 2, the stimulation levels for obtaining the sample are nonirritant.
4. detection method according to claim 3, which is characterized in that described according to the single vessel extent of reaction mean value
Judge that the step of obtaining the stimulation levels of the sample further includes：
When positive reaction incidence is more than probability threshold value, the correction stimulus index respectively with the first stimulus threshold, the
Size between two stimulus thresholds, third stimulus threshold and the 4th stimulus threshold；
If the correction stimulus index is more than 0 and is less than or equal to the first stimulus threshold, the stimulation levels of the sample are obtained
For pole slight stimulation；
If the correction stimulus index is more than the first stimulus threshold and is less than or equal to the second stimulus threshold, the sample is obtained
Stimulation levels be slight stimulation；
If the correction stimulus index is more than the second stimulus threshold and is less than or equal to third stimulus threshold, the sample is obtained
Stimulation levels be moderate irritation；
If the correction stimulus index is more than third stimulus threshold and is less than or equal to the 4th stimulus threshold, the sample is obtained
Stimulation levels be strong and stimulating.
5. according to claim 1-4 any one of them detection methods, which is characterized in that the solid is non-aqueous/oil-soluble film quality
Material includes mask substrate, face paper, film cloth, face paste formula facial mask, membrane material, medical dressing, disinfected paper napkin, female sanitary towel and baby
Youngster's diaper.
6. a kind of solid is non-aqueous/the irritating detection device of oil-soluble Membranes, which is characterized in that including：
First acquisition unit, for obtaining the first blood vessel state data in each fertilization chick chorioallantoic membrane detection zone；
Second acquisition unit, for obtain by each solid it is non-aqueous/samples of oil-soluble Membranes spreads on institute correspondingly
It states in detection zone after effect a period of time and removes the second blood vessel state data in the detection zone of the sample；
First compare with vascular reaction degree result acquiring unit, for the first blood vessel state data described in being respectively compared every group and
Second blood vessel state data obtain the vascular reaction degree result in each detection zone；
First computing unit obtains single blood for being calculated according to the vascular reaction degree result in the detection zone
Tube reaction degree mean value；
Stimulation levels acquiring unit, the stimulation etc. for judging the acquisition sample according to the single vessel extent of reaction mean value
Grade.
7. detection device according to claim 6, which is characterized in that described first compares and obtained with vascular reaction degree result
The unit is taken to include：
First judging unit, for judging that the second blood vessel state data are compared with the variation of the first blood vessel state data described in every group respectively
Degree；
First vascular reaction degree result acquiring unit obtains the blood vessel that effect value is equal to 0 if reactionless for blood vessel state
Extent of reaction result；
Second vascular reaction degree result acquiring unit obtains effect value if being that blood vessel does not flow for blood vessel state
Vascular reaction degree result equal to 1；
Third vascular reaction degree result acquiring unit, if being more than 0 for congested value range and being less than or equal to first threshold,
Obtain the vascular reaction degree result that effect value is equal to 2；
4th vascular reaction degree result acquiring unit, if being more than first threshold for congested value range and being less than or equal to second
Threshold value then obtains the vascular reaction degree result that effect value is equal to 3；
5th vascular reaction degree result acquiring unit, if being more than second threshold for congested value range and being less than or equal to third
Threshold value then obtains the vascular reaction degree result that effect value is equal to 4；
6th vascular reaction degree result acquiring unit obtains effect value etc. if being more than third threshold value for congested value range
In 5 vascular reaction degree result；
7th vascular reaction degree result acquiring unit, if being more than 0 for bleeding value range and being less than or equal to the 4th threshold value,
Obtain the vascular reaction degree result that effect value is equal to 6；
8th vascular reaction degree result acquiring unit obtains effect value etc. if being more than the 4th threshold value for bleeding value range
In 7 vascular reaction degree result.
8. detection device according to claim 7, which is characterized in that the stimulation levels acquiring unit includes：
Second computing unit, for being obtained according to the single vessel extent of reaction mean value and single vessel reaction Incidence calculus
Vascular stimulation index；
Third computing unit, for the vascular stimulation index to be compared calculating with the vascular stimulation index of negative control,
Obtain correction stimulus index；
Second judgment unit, for judging whether positive reaction incidence is less than or equal to probability threshold value, the positive reaction is
Effect value is more than 0 vascular reaction degree result；
Third judging unit, for when positive reaction incidence is less than or equal to probability threshold value, judging that the correction stimulation refers to
Whether number is less than 2；
The first estate acquiring unit, the stimulation levels for when correcting stimulus index less than 2, obtaining the sample are non-stimulated
Property.
9. detection device according to claim 8, which is characterized in that the stimulation levels acquiring unit further includes：
Comparing unit, for when positive reaction incidence is more than probability threshold value, the correction stimulus index is respectively with the
Size between one stimulus threshold, the second stimulus threshold, third stimulus threshold and the 4th stimulus threshold；
Second grade acquiring unit obtains if being more than 0 for the correction stimulus index and being less than or equal to the first stimulus threshold
The stimulation levels for obtaining the sample are pole slight stimulation；
Tertiary gradient acquiring unit, if being more than the first stimulus threshold for the correction stimulus index and less than or equal to the second thorn
Swash threshold value, then the stimulation levels for obtaining the sample are slight stimulation；
Fourth estate acquiring unit, if being more than the second stimulus threshold for the correction stimulus index and being pierced less than or equal to third
Swash threshold value, then the stimulation levels for obtaining the sample are moderate irritation；
5th grade acquiring unit, if being more than third stimulus threshold for the correction stimulus index and less than or equal to the 4th thorn
Swash threshold value, then the stimulation levels for obtaining the sample are strong and stimulating.
10. a kind of solid is non-aqueous/the irritating detecting system of oil-soluble Membranes, which is characterized in that including such as claim
Any one of 6-9 detection devices and observation recorder；
The observation recorder is connect with the detection device, for the image in acquisition testing region and is transferred to the detection
Device.
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