CN108593605A - Three-dimensional high-speed wide visual field chromatography imaging method and device - Google Patents
Three-dimensional high-speed wide visual field chromatography imaging method and device Download PDFInfo
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Abstract
The present invention proposes a kind of three-dimensional high-speed wide visual field chromatography imaging method and device, wherein method includes:Beam generates step, for generating light beam;High-speed space-time focusing surface scanning step, to the light beam carry out dispersion with after parallel collimation, apply different phases to the light beam of different wave length/frequency to be modulated, light beam is made to generate different time delays, modulated light beam is converged on sample to form focusing surface;Depth of field detection steps are expanded, to the light that the sample is excited, high speed variable focal length lenses are added at the conjugate planes in object lens entrance pupil face, and then realize the imaging synchronous with excitation end axial scan to the object plane of different depth;Synchronous control step, the high speed variable focal length lenses that the light velocity in high-speed space-time focusing surface scanning step is modulated and expanded in depth of field detection steps synchronize control.The method of the present invention can improve image taking speed, realize the three-dimensional imaging of high time resolution under the premise of ensureing spatial resolution.
Description
Technical field
The present invention relates to optical microscopy field more particularly to a kind of three-dimensional high-speed wide visual field chromatography imaging methods and dress
It sets.
Background technology
Optical microscopy imaging technology is the technology for the high-definition picture for optically obtaining object, is widely used in
The structure imaging and function signal of micro-object detect, it has also become the common method of biological study at present.It is existing it is micro- at
As in technology, common wide field single photon image penetration depth is small, and does not have chromatography ability;Although layer may be implemented in mating plate imaging
Analysis imaging, but can not be applied in the sample of strong scattering medium.Multi-photon Mapping technology is imitated using nonlinear optics
Local signal stress be sent out, there is stronger chromatography ability.In addition, multi-photon Mapping technology uses long-wavelength excitation, it can
Realize deeper penetration depth, but since it is based on point-scan mode, temporal resolution is difficult to meet some actual demands.
In order in scattering biological tissue realize high speed wide visual field tomography, pertinent literature proposes and developed space-time gather
Burnt technology.The basic principle of the technology is to carry out shaping to the time dimension of light pulse based on Multi-photon Nonlinear effect, from
And so that light pulse only reaches most short pulse width at focusing surface, obtains highest multiphoton excitation efficiency.Relative to mostly light
Sub- Mapping technology, the technology can excite the region of certain area parallel, improve image taking speed.Specific method is,
Light pulse is broadened in time using Dispersive Devices first, makes power dissipation, will be broadened by collimation lens and object lens later
Light pulse assembled on focusing surface again, to based on nonlinear optical effect realize wide field chromatography excitation.Relative to common
Multi-photon wide field exciting method, due to introducing the time dimension of light pulse, this method have higher axial resolution and
Higher signal-to-noise ratio.
In the imaging of practical Photobiology, in order to study biological dynamic process, need to carry out three-dimensional high-speed imaging.It is intended to use
Space-time focus method carries out three-dimensional high-speed imaging, generally require to target volume carry out high speed successively scan and realize it is synchronous at
Picture.Regrettably, although there has been proposed the axial scanning method that some are focused based on space-time, corresponding axis is not suggested that
To synchronizing detection method, i.e., currently there is not yet the report of relevant three-dimensional high-speed wide visual field chromatography imaging method.Specifically, existing
It is mainly that light pulse is changed to optical dispersions devices such as, gratings by adjusting prism based on the axial scanning method that space-time focuses
Group velocity dispersion, and then change focusing surface and realize scanning.Change group velocity dispersion the result is that so that after dispersion different frequency
The distance between light beam changes, so that the change of light pulse converged position, i.e. focusing surface change in location.However, above-mentioned
The approach for changing group velocity dispersion is constrained by machinery inertial, limits the speed of axial scan.In addition, for realize clearly at
Picture, end of probe image sensor (such as CCD, sCMOS) needs synchronizing moving, to keep the object-image relation of focusing surface and image planes, and it is high
Fast Mechanical Moving image sensor is unpractical, therefore three-dimensional high-speed wide visual field chromatography imaging technique speed is still unhappy.
Invention content
The present invention is directed to solve at least some of the technical problems in related technologies.For this purpose, the present invention
One side purpose is that proposition one kind can be under the premise of ensureing system performance, the three-dimensional high-speed wide visual field of lifting body image taking speed
Chromatography imaging method.
Another aspect of the present invention purpose is to propose a kind of three-dimensional high-speed wide visual field laminated imaging device.
In order to achieve the above objectives, the embodiment of one aspect of the present invention proposes a kind of three-dimensional high-speed wide visual field tomography side
Method includes the following steps:Light beam generates step, for generating light beam;High-speed space-time focusing surface scanning step, to the light beam into
Row dispersion applies different phases to the light beam of different wave length/frequency and is modulated with after parallel collimation, so that light beam is generated different
Time delay, modulated light beam is converged on sample to form focusing surface;Depth of field detection steps are expanded, to the sample
High speed variable focal length lenses are added in the light excited at the conjugate planes in object lens entrance pupil face, and then to the object plane of different depth reality
The now imaging synchronous with excitation end axial scan;Synchronous control step, to the light velocity tune in high-speed space-time focusing surface scanning step
System and the high speed variable focal length lenses expanded in depth of field detection steps synchronize control, to realize lighting position and detecting location
Synchronization.
Three-dimensional high-speed wide visual field chromatography imaging method according to the ... of the embodiment of the present invention, by being diffused to light beam and accurate
Directly, it carries out phase-modulation and converges to form focal plane on sample;Then the light excited to sample carries out high speed zoom, to difference
The object plane synchronous imaging of depth, and synchronous lighting position and detecting location, to reach the not mobile system optical device the case where
Under, ensure imaging space resolution ratio and illumination efficiency, and improve the purpose of the temporal resolution of body imaging.
In some instances, the light beam generates step, including:The light that pulsed laser light source is sent out is collimated and expanded
It dissipates, to generate the light beam.
The embodiment of another aspect of the present invention proposes a kind of three-dimensional high-speed wide visual field laminated imaging device, including:Light
Beam generation device, for generating light beam;High-speed space-time focusing surface scanning means carries out dispersion and parallel collimation to the light beam
Afterwards, apply different phases to the light beam of different wave length/frequency to be modulated, light beam is made to generate different time delays, will adjust
Light beam after system converges on sample to form focusing surface;Depth of field detection device is expanded, to the light that the sample is excited, in object
High speed variable focal length lenses are added at the conjugate planes in mirror entrance pupil face, and then the object plane of different depth is realized and is axially swept with excitation end
Retouch synchronous imaging;Controller is modulated the light velocity in high-speed space-time focusing surface scanning step and is expanded in depth of field detection steps
High speed variable focal length lenses synchronize control, to realize that lighting position is synchronous with detecting location.
Three-dimensional high-speed wide visual field laminated imaging device according to the ... of the embodiment of the present invention, by being diffused to light beam and accurate
Directly, it carries out phase-modulation and converges to form focal plane on sample;Then the light excited to sample carries out high speed zoom, to difference
The object plane synchronous imaging of depth, and synchronous lighting position and detecting location, to reach the not mobile system optical device the case where
Under, ensure imaging space resolution ratio and illumination efficiency, and improve the purpose of the temporal resolution of body imaging.
In some instances, the high-speed space-time focusing surface scanning means includes:Grating 201, collimation lens 202, space
Optical modulator 203, the first speculum 204, the first lens 205, the second lens 206 and the first object lens 207.
Wherein, light beam passes through the dispersion on grating S201, light beam after dispersion by collimation lens 202 and spectrum with space
The beamlet of distribution, different frequency passes through 203 phase modulation of spatial light modulator, to change the group velocity dispersion of light pulse,
Modulated light beam is reflected by the first speculum 204, by the 4f systems being made of the first lens 205 and the second lens 206
It is collected and assembles by the first object lens 207 afterwards, focusing surface is formed on sample 301.
Wherein, the phase to sub- beam modulation, the group velocity dispersion of light pulse are changed at a high speed by spatial light modulator 203
High speed occurs to change, and then makes the focusing surface after convergence that high speed axial movement occur and realizes scanning.
In some instances, the expansion depth of field detection steps, including:Second object lens 302, dichroscope 303, third are saturating
Mirror 304, the 4th lens 305, Zoom lens 306, tube lens 307 and camera 308.
Wherein, sample 301 be stimulated generation optical signal by the second object lens 302 collection after, reflected by dichroscope 303
By the relaying light path being made of the third lens 304 and the 4th lens 305, then light beam is varifocal by Zoom lens 306
Lens are realized that focal length synchronizes by the electric signal driving synchronous with excitation end axial scan and are changed, and light beam is passed through tube lens
It is received by camera 308 after 307 convergences, realizes extended depth-of-field detection.
In some instances, the beam generated device includes:Pulsed laser light source 401, electrooptic modulator 402, collimation
Beam expander 403 and the second speculum 404.
Wherein, the light that pulsed laser light source 401 is sent out incident collimation after light intensity is adjusted in electrooptic modulator 402
Beam expander 403 is collimated, is expanded, and reflexes to high-speed space-time focusing surface scanning means by the second speculum 404.
The additional aspect of the present invention and advantage will be set forth in part in the description, and will partly become from the following description
Obviously, or practice through the invention is recognized.
Description of the drawings
Fig. 1 is the flow chart of three-dimensional high-speed wide visual field chromatography imaging method according to the ... of the embodiment of the present invention;
Fig. 2 is the structural schematic diagram of three-dimensional high-speed wide visual field laminated imaging device according to the ... of the embodiment of the present invention;
Fig. 3 is the structural schematic diagram of the space-time focusing surface scanning means of one embodiment of the invention;
Fig. 4 is the structural schematic diagram of the expansion depth of field detection device of one embodiment of the invention;With
Fig. 5 is three-dimensional high-speed wide visual field laminated imaging device schematic diagram according to an embodiment of the invention.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
It is according to the flow chart of the three-dimensional high-speed wide visual field chromatography imaging method of one embodiment of the invention, originally referring to Fig. 1
The three-dimensional high-speed wide visual field chromatography imaging method of invention one embodiment, includes the following steps:
S1, light beam generates step, for generating light beam.
Specifically, the light that pulsed laser light source is sent out is collimated and is spread, to generate multiple collimated light beams.
S2, high-speed space-time focusing surface scanning step, to light beam carry out dispersion with after parallel collimation, to different wave length/frequency
Light beam apply different phases and be modulated, so that light beam is generated different time delays, modulated light beam converged into sample
To form focusing surface on product.
It is different from conventional space-time focus illumination light path as a specific example, laser pulse after Dispersive Devices,
Applying different phases to the light beam of different wave length (frequency) using optical modulation device makes it generate different time delays, that is, draws
Enter tunable group velocity dispersion, realizes the high speed axial scan of focusing surface.
S3 expands depth of field detection steps, and to the light that sample is excited, high speed is added at the conjugate planes in object lens entrance pupil face can
Variable focal length lens, and then the imaging synchronous with excitation end axial scan is realized to the object plane of different depth.
Specifically, different from conventional space-time focal imaging light path, high speed is added at the conjugate planes in object lens entrance pupil face can
Variable focal length lens, and then the blur-free imaging synchronous with excitation end axial scan is realized to the object plane of different depth.
S4, synchronous control step modulate the light velocity in high-speed space-time focusing surface scanning step and expand depth of field detection step
High speed variable focal length lenses in rapid synchronize control, to realize that lighting position is synchronous with detecting location.
Three-dimensional high-speed wide visual field chromatography imaging method according to the ... of the embodiment of the present invention is carried out accurate by the light sent out to light source
Straight and diffusion generates light beam, carries out dispersion to dispersed light beam and collimates and carry out phase-modulation, converges to and form focal plane on sample;
Then it allows the light that sample is excited by high speed variable focal length lenses, blur-free imaging, while to realization lighting position and detecting position
It sets and synchronizes control, to reach in the case of not mobile system optical device, ensure that imaging space resolution ratio and illumination are imitated
Rate, and improve the purpose of the temporal resolution of body imaging.
In addition, embodiment of the invention discloses that three-dimensional high-speed wide visual field laminated imaging device, as shown in Fig. 2, being this hair
The schematic diagram of the three-dimensional high-speed wide visual field laminated imaging device of bright embodiment, the three-dimensional high-speed wide visual field chromatography of the embodiment of the present invention
Imaging device 10, including:Beam generated device 101, expands depth of field detection device 103 at high-speed space-time focusing surface scanning means 102
With controller 104.
Wherein, beam generated device 101 is for generating light beam;High-speed space-time focusing surface scanning means 102, for light beam
Dispersion is carried out with after parallel collimation, different phases is applied to the light beam of different wave length/frequency and is modulated, light beam is made to generate not
Same time delay, modulated light beam is converged on sample to form focusing surface;Depth of field detection device 103 is expanded, it can
To the light that sample is excited, high speed variable focal length lenses are added at the conjugate planes in object lens entrance pupil face, and then to different depth
Object plane realizes the imaging synchronous with excitation end axial scan;The effect of controller 104 is in high-speed space-time focusing surface scanning step
The light velocity modulation and expand depth of field detection steps in high speed variable focal length lenses synchronize control, with realize lighting position with
The synchronization of detecting location.
Individually below to beam generated device 101, high-speed space-time focusing surface scanning means 102, expansion depth of field detection device
103 and controller 104 be described in detail.
Wherein, beam generated device 101, for generating light beam.
As shown in figure 5, as a specific example, beam generated device 101 includes:Pulsed laser light source 401, electric light tune
Device 402, collimator and extender device 403 and the second speculum 404 processed, wherein the light that pulsed laser light source 401 is sent out passes through Electro-optical Modulation
Device 402 after light intensity is adjusted incident collimator and extender device 403 collimated, expanded, and reflected by the second speculum 404 supreme
Fast space-time focusing surface scanning means 102.Light source is modulated, collimate, is expanded, and is reflected into high-speed space-time and focuses Surface scan dress
Set 102.
High-speed space-time focusing surface scanning means 102, for light beam carry out dispersion with after parallel collimation, to different wave length/
The light beam of frequency applies different phases and is modulated, and light beam is made to generate different time delays, and modulated light beam is assembled
To form focusing surface on to sample.As shown in figure 3, high-speed space-time focusing surface scanning means 102, it can be by Dispersive Devices, collimation
Lens, optical modulation device and object lens constitute the light of laser pulse different wave length after Dispersive Devices in space development, by standard
Straight lens make the parallel collimation of the light beam of different wave length, and the light beam after collimation is by optical modulation device to the light of different wave length (frequency)
Beam is modulated, and modulated light beam is focused on sample again by object lens.
In conjunction with shown in Fig. 2 and 3, in a specific example, high-speed space-time focusing surface scanning means 102 includes:Grating
201, collimation lens 202, spatial light modulator 203, the first speculum 204, the first lens 205, the second lens 206 and the first object
Mirror 207, wherein light beam passes through the dispersion on grating S201, light beam after dispersion by collimation lens 202 and spectrum with space point
The beamlet of cloth, different frequency passes through 203 phase modulation of spatial light modulator, to change the group velocity dispersion of light pulse, adjusts
Light beam after system is reflected by the first speculum 204, after the 4f systems being made of the first lens 205 and the second lens 206
It is collected and assembles by the first object lens 207, focusing surface is formed on sample 301.Wherein, changed at a high speed by spatial light modulator 203
To the phase of sub- beam modulation, the group velocity dispersion of light pulse occurs high speed and changes, and then it is high so that the focusing surface after convergence is occurred
Scanning is realized in speed axial movement.High-speed space-time focusing surface scanning means 102 is that light beam exists according to the different wave length of light beam after dispersion
Space development carries out phase-modulation after collimation, and converges on sample again.
As shown in figure 4, expand depth of field detection device 103, the light excited for sample into line convergence to form image planes, and
Phase-modulation is carried out in image space focal plane or conjugate planes opposite surface to be imaged with the object plane to different depth.Wherein, scape is expanded
Deep investigation device 103 can be made of object lens, tube lens, lens group, light phase modulation device and photodetector etc..It expands
The light path of depth of field detection device 103 is collected in the light of object emission by object lens, assembles to form image planes by lens.Add after image planes
Enter and relay light path, and place optical phase modulator part in the conjugate planes in object lens entrance pupil face and carry out phase-modulation, in being total to for object plane
Yoke surface places photodetector and carries out detection imaging.
As a specific example, as shown in figure 4, the knot of the expansion depth of field detection device for one embodiment of the invention
Structure schematic diagram, as shown in figure 4, depth of field detection device 10 is expanded, including:Second object lens 302, dichroscope 303, the third lens
304, the 4th lens 305, Zoom lens 306, tube lens 307 and camera 308, wherein sample 301 is stimulated the light of generation
Signal is after the collection of the second object lens 302, by the reflection of dichroscope 303 by being made of the third lens 304 and the 4th lens 305
Relaying light path, then for light beam by Zoom lens 306, Zoom lens pass through the telecommunications synchronous with excitation end axial scan
Number driving realizes that focal length synchronizes variation, and light beam is received after the convergence of tube lens 307 by camera 308, realizes extended depth-of-field
Detection.The expansion depth of field detection device can collect the light of object emission, the linear imaging surface of converging light, and then carry out phase to image planes
Position modulation, and place camera in the conjugate planes of object plane and carry out detection imaging, have the function that expand the depth of field.
Controller 104, for depth of field detection steps to be modulated and expanded to the light velocity in high-speed space-time focusing surface scanning step
In high speed variable focal length lenses synchronize control, to realize that lighting position is synchronous with detecting location.
As a specific example, as shown in figure 5, for according to the three-dimensional high-speed wide visual field layer of one embodiment of the invention
Analyse imaging device schematic diagram.Wherein, controller 104 synchronizes control to spatial light modulator 203 and Zoom lens 306,
Achieve the effect that lighting position is synchronous with detecting location.
As shown in figure 5, focusing Surface scan for beam generated device 101, the high-speed space-time described respectively in above example
Device 102, the three-dimensional high-speed wide visual field laminated imaging device for expanding depth of field detection device 103 and the composition of controller 104.At one
In specific example, imaging process is:
In conjunction with Fig. 5, pass through electrooptic modulator 402, collimator and extender device 403 successively in the laser of light source 401, light beam is accurate
Directly, scanning focused on sample 301 by high-speed space-time focusing surface by the transmission of speculum 404 on grating 405 after expanding.
Sample 301 is excited the signal light generated by expanding depth of field device, and wherein controller 104 to spatial light modulator 203 and can be changed
Focus lens 306 synchronize control, finally (i.e. by 308sCMOS:Camera 308) acquisition imaging.When light source is through ovennodulation, high speed
Empty focusing surface is scanning focused and expands depth of field detection, and scape is modulated and expanded to the light velocity in high-speed space-time focusing surface scanning step
High speed variable focal length lenses in deep investigation step synchronize control, complete three-dimensional high-speed wide visual field tomography.
It should be noted that the aforementioned explanation to three-dimensional high-speed wide visual field chromatography imaging method embodiment is also applied for
The device of the three-dimensional high-speed wide visual field tomography embodiment, details are not described herein again.
A kind of three-dimensional high-speed wide visual field laminated imaging device according to the ... of the embodiment of the present invention, by light that light source is sent out into
Row collimation and diffusion generate light beam, carry out dispersion to dispersed light beam and collimate and carry out phase-modulation, converge on sample and formed
Focal plane;Then allow the light that sample is excited by high speed variable focal length lenses, blur-free imaging, while to realizing lighting position and spy
Location, which is set, synchronizes control, to reach in the case of not mobile system optical device, ensures imaging space resolution ratio and photograph
Obvious results rate, and improve the purpose of the temporal resolution of body imaging.
In the description of the present invention, it is to be understood that, term "center", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on ... shown in the drawings or
Position relationship is merely for convenience of description of the present invention and simplification of the description, and does not indicate or imply the indicated device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are used for description purposes only, it is not understood to indicate or imply relative importance
Or implicitly indicate the quantity of indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the present invention, the meaning of " plurality " is at least two, such as two, three
It is a etc., unless otherwise specifically defined.
In the present invention unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral;Can be that machinery connects
It connects, can also be electrical connection;It can be directly connected, can also can be indirectly connected through an intermediary in two elements
The interaction relationship of the connection in portion or two elements, unless otherwise restricted clearly.For those of ordinary skill in the art
For, the specific meanings of the above terms in the present invention can be understood according to specific conditions.
In the present invention unless specifically defined or limited otherwise, fisrt feature can be with "above" or "below" second feature
It is that the first and second features are in direct contact or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists
Second feature " on ", " top " and " above " but fisrt feature be directly above or diagonally above the second feature, or be merely representative of
Fisrt feature level height is higher than second feature.Fisrt feature second feature " under ", " lower section " and " below " can be
One feature is directly under or diagonally below the second feature, or is merely representative of fisrt feature level height and is less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiments or example.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changes, replacing and modification.
Claims (6)
1. a kind of three-dimensional high-speed wide visual field chromatography imaging method, which is characterized in that include the following steps:
Light beam generates step, for generating light beam;
High-speed space-time focusing surface scanning step, to the light beam carry out dispersion with after parallel collimation, to the light of different wave length/frequency
Beam applies different phases and is modulated, and light beam is made to generate different time delays, and modulated light beam is converged on sample
To form focusing surface;
Depth of field detection steps are expanded, to the light that the sample is excited, high speed variable is added at the conjugate planes in object lens entrance pupil face
Focal length lenses, and then the imaging synchronous with excitation end axial scan is realized to the object plane of different depth;
Synchronous control step modulates and expands the height in depth of field detection steps to the light velocity in high-speed space-time focusing surface scanning step
Fast lens of variable focal length synchronizes control, to realize that lighting position is synchronous with detecting location.
2. three-dimensional high-speed wide visual field chromatography imaging method according to claim 1, which is characterized in that the light beam generates step
Suddenly, including:The light that pulsed laser light source is sent out is collimated and spread, to generate the light beam.
3. a kind of three-dimensional high-speed wide visual field laminated imaging device, which is characterized in that including:
Beam generated device, for generating light beam;
High-speed space-time focusing surface scanning means, to the light beam carry out dispersion with after parallel collimation, to the light of different wave length/frequency
Beam applies different phases and is modulated, and light beam is made to generate different time delays, and modulated light beam is converged on sample
To form focusing surface;
Depth of field detection device is expanded, to the light that the sample is excited, high speed variable is added at the conjugate planes in object lens entrance pupil face
Focal length lenses, and then the imaging synchronous with excitation end axial scan is realized to the object plane of different depth;
Controller modulates and expands the high speed variable in depth of field detection steps to the light velocity in high-speed space-time focusing surface scanning step
Focal length lenses synchronize control, to realize that lighting position is synchronous with detecting location.
4. three-dimensional high-speed wide visual field laminated imaging device according to claim 3, which is characterized in that the high-speed space-time is poly-
Focal plane scanning means includes:Grating 201, collimation lens 202, spatial light modulator 203, the first speculum 204, the first lens
205, the second lens 206 and the first object lens 207,
Wherein, light beam passes through the dispersion on grating 201, light beam after dispersion by collimation lens 202 and spectrum with spatial distribution,
The beamlet of different frequency passes through 203 phase modulation of spatial light modulator, to change the group velocity dispersion of light pulse, after modulation
Light beam reflected by the first speculum 204, by the after the 4f systems being made of the first lens 205 and the second lens 206
One object lens 207, which are collected, to be assembled, and focusing surface is formed on sample 301.
Wherein, the phase to sub- beam modulation is changed at a high speed by spatial light modulator 203, the group velocity dispersion of light pulse occurs
High speed changes, and then makes the focusing surface after convergence that high speed axial movement occur and realize scanning.
5. three-dimensional high-speed wide visual field laminated imaging device according to claim 3, which is characterized in that the expansion depth of field is visited
Step is surveyed, including:Second object lens 302, dichroscope 303, the third lens 304, the 4th lens 305, Zoom lens 306, mirror
Cylinder lens 307 and camera 308,
Wherein, sample 301 be stimulated generation optical signal by the second object lens 302 collection after, by dichroscope 303 reflect pass through
The relaying light path being made of the third lens 304 and the 4th lens 305, then light beam pass through Zoom lens 306, Zoom lens
It realizes that focal length synchronizes by the electric signal driving synchronous with excitation end axial scan to change, light beam is passed through into 307 meeting of tube lens
It is received by camera 308 after poly-, realizes extended depth-of-field detection.
6. three-dimensional high-speed wide visual field laminated imaging device according to claim 3, which is characterized in that the light beam generates dress
Set including:Pulsed laser light source 401, electrooptic modulator 402, collimator and extender device 403 and the second speculum 404,
Wherein, the light that pulsed laser light source 401 is sent out incident collimator and extender after light intensity is adjusted in electrooptic modulator 402
Device 403 is collimated, is expanded, and reflexes to high-speed space-time focusing surface scanning means by the second speculum 404.
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