CN108589045A - A kind of nano fibrous membrane and preparation and application loading prebiotics and/or probiotics - Google Patents
A kind of nano fibrous membrane and preparation and application loading prebiotics and/or probiotics Download PDFInfo
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- CN108589045A CN108589045A CN201810314108.6A CN201810314108A CN108589045A CN 108589045 A CN108589045 A CN 108589045A CN 201810314108 A CN201810314108 A CN 201810314108A CN 108589045 A CN108589045 A CN 108589045A
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- probiotics
- prebiotics
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/413—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties containing granules other than absorbent substances
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
- D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
- D04H1/4282—Addition polymers
- D04H1/4309—Polyvinyl alcohol
Abstract
The invention belongs to technical field of functional food, disclose a kind of nano fibrous membrane and preparation and application loading prebiotics and/or probiotics.Polyvinyl alcohol is dissolved in aseptic deionized water, polyvinyl alcohol spinning solution is obtained；Prebiotics are added into polyvinyl alcohol spinning solution again, stir evenly, obtain polyvinyl alcohol/prebiotics spinning solution；Probiotics bacterial liquid is added in polyvinyl alcohol spinning solution or polyvinyl alcohol/prebiotics spinning solution, is stirred evenly, polyvinyl alcohol/probiotics spinning solution or polyvinyl alcohol/prebiotics/probiotics spinning solution are obtained；Load prebiotics and/or the nano fibrous membrane of probiotics are prepared by electrostatic spinning in gained spinning solution.Present invention load prebiotics and/or the nano fibrous membrane of probiotics can largely improve the activity and thermal stability of probiotics.
Description
Technical field
The invention belongs to technical field of functional food, and in particular to a kind of Nanowire loading prebiotics and/or probiotics
Tie up film and preparation and application.
Background technology
Probiotics is also known as active bacteria formulation, microecologic regulator, ecological product, regulator etc., and host can be promoted by being one kind
The ecological balance of gut microflora generates host health and/or physiological function the active microorganism of beneficial effect.So
And probiotics its prebiotic function of competence exertion when only taking in a certain amount of, and food processing, storage and digestive environments can all influence
The activity of probiotics.Make probiotics not only in production and processing, packing box storage requirement it would therefore be highly desirable to develop a kind of new technology
Lower survival, and can also survive when passing through gastrointestinal tract, to make probiotics play its positive health promotion effect.
In recent years, embedding techniques have been widely used for improving probiotics unfavorable as a kind of maximally efficient Preservation tactics
Survival rate in environment.The wherein selection of embedding method is most important to the protective effect for embedding system.As nanotechnology is ground
That studies carefully deepens continuously, and method of electrostatic spinning receives highest attention in active material embedding field.(spraying is dry with other embedding techniques
Dry method, emulsion process, extrusion etc.) it compares, method of electrostatic spinning is that a kind of preparation condition is mild, simple for process and can continuously prepare and receive
The method of rice fiber, gained nano fibrous membrane large specific surface area, porosity is high, high to active material load factor and with good
Controlled release properties.
Prebiotics are the non-starch polysaccharide in plant, it cannot be decomposed by the enzyme system in host, but can pass through selection
Property stimulate the growth of intestinal microflora and activity and wholesome effect generated to host, so as to improve the healthy water of host
It is flat.Although embedding techniques can improve the survival rate of probiotics to a certain extent, when probiotics only takes in a certain amount of
Prebiotic function can be played.But there has been no the system reports about prebiotics electrostatic spinning at present.
Invention content
In place of the above shortcoming and defect of the existing technology, the primary purpose of the present invention is that providing a kind of load
The preparation method of prebiotics and/or the nano fibrous membrane of probiotics.
Another object of the present invention is to provide a kind of nano fibrous membranes being prepared by the above method.
It is still another object of the present invention to provide above-mentioned load prebiotics and/or the nano fibrous membrane of probiotics in functionality
Application in food.
The object of the invention is achieved through the following technical solutions：
A kind of preparation method for the nano fibrous membrane loading prebiotics and/or probiotics, includes the following steps：
(1) polyvinyl alcohol is dissolved in aseptic deionized water, obtains polyvinyl alcohol spinning solution；Again to polyvinyl alcohol spinning solution
Middle addition prebiotics, stir evenly, and obtain polyvinyl alcohol/prebiotics spinning solution；
(2) the polyvinyl alcohol spinning solution or polyvinyl alcohol/prebiotics spinning solution being added to probiotics bacterial liquid in step (1)
In, it stirs evenly, obtains polyvinyl alcohol/probiotics spinning solution or polyvinyl alcohol/prebiotics/probiotics spinning solution；
(3) load prebiotics and/or benefit are prepared by electrostatic spinning in spinning solution obtained by step (1) or step (2)
The nano fibrous membrane of raw bacterium.
Preferably, the aseptic deionized water described in step (1) refers to after 115~121 DEG C of 15~20min of high-temperature sterilization
Deionized water.
Preferably, the mass concentration of polyvinyl alcohol spinning solution described in step (1) is 6%~10%.
Preferably, prebiotics described in step (1), which include oligosaccharide, polysaccharide, polyphenol, flavones etc., can promote probiotics
At least one of the substance for rising in value or improving its vigor.The additive amount of the prebiotics is polyvinyl alcohol/prebiotics spinning solution
The 0.5%~3% of quality.
Preferably, the probiotics described in step (2) refers at least one of Lactobacillus and Bifidobacterium；Institute
State a concentration of the 10 of probiotics bacterial liquid8~1010CFU/mL。
Preferably, the addition of probiotics bacterial liquid described in step (2) and polyvinyl alcohol spinning solution or polyvinyl alcohol/prebiotic
The volume ratio of first spinning solution is 1:20.
Preferably, the condition of electrostatic spinning described in step (3) is：Spinning flow velocity is 0.2~1.0mL/h, and syringe needle is straight
0.4~0.6mm of diameter, voltage be 10~20kV, receive distance be 8~14cm, temperature be 20~25 DEG C, humidity be 35%~
55%.
A kind of nano fibrous membrane loading prebiotics and/or probiotics, is prepared by the above method.
Preferably, the fibre diameter of the load prebiotics and/or the nano fibrous membrane of probiotics is 210~1040nm.
The application of above-mentioned load prebiotics and/or the nano fibrous membrane of probiotics in functional food.
The present invention preparation method and obtained product has the following advantages that and advantageous effect：
(1) present invention embedding probiotics using prebiotics as electrospun material for the first time, system research prebiotics additions
The influence of influence and electrostatic spinning to prebiotics probiotic effects to electrostatic spinning.The addition of prebiotics significantly improves probiotics
Survival rate, add probiotics survival rate in the more un-added tunica fibrosa of tunica fibrosa of prebiotics and increase 1.1logs, equally contain
There is the spinning solution of prebiotics to increase 0.7logs compared with probiotics survival rate in the spinning solution without prebiotics.
(2) present invention embeds probiotics, the used voltage of electrostatic spinning using green, mild electrostatic spinning technique
(10~16kV) has no significant effect (ANOVA, P to the activity of probiotics>0.05).The present invention uses electrostatic spinning technique with prebiotic
Member embeds the embedding that the composite nano-fiber membrane that probiotics is prepared is not only functional mass for wall material and provides one newly
Form, while electrostatic spinning technique has also been expanded in the application space of food industry.In addition, electrostatic spinning technique it is simple for process,
It is economic and environment-friendly, compared with other embedding techniques, while ensureing activity of probiotic, electrospun fibers film large specific surface area,
Porosity is high, and the High efficient encapsulation and controlled release of active material may be implemented.
Description of the drawings
Fig. 1 be different three kinds of oligosaccharide probiotics of probiotics pair in embodiment 1 utilization power comparison diagram (A1,
L.casei；A2, L.paracasei；A3, L.plantarum；A4, L.acidophilus；B1, B.adolescence；B2,
B.bifidum；Glc, glucose；FOS, oligofructose；GOS, galactooligosaccharide；IMO, oligoisomaltose；Control, it is empty
White control).
Fig. 2 is that four kinds of Bacillus acidi lacticis of oligofructose additive amount pair of various concentration and two kinds of Bifidobacterium probiotic effects compare
Scheme (A, L.casei；B,L.paracasei；C,L.plantarum；D,L.acidophilus；E,B.adolescence；F,
B.bifidum)。
Fig. 3 be different spinning voltages under the conditions of nano fibrous membrane in lactobacillus plantarum Activity Results figure.
Fig. 4 is that the SEM of the nano fibrous membrane of gained load oligofructose and lactobacillus plantarum in embodiment 5 schemes and fiber is straight
Diameter distribution map.
Fig. 5 is that embodiment 3 (PVA/FOS, 2.5wt.%FOS) and embodiment 5 (PVA/FOS/L.plantarum) obtain
Nano fibrous membrane, pva powder (PVA), oligofructose powder (FOS) and lactobacillus plantarum thalline (L.plantarum)
Infrared spectrogram.
Fig. 6 be the obtained nano fibrous membrane (PVA/FOS/L.plantarum) of embodiment 5 and pva powder (PVA),
Thermogravimetric analysis figure (A, the TGA of oligofructose powder (FOS), lactobacillus plantarum thalline (L.plantarum)；B,DTG).
Fig. 7 be nano fibrous membrane containing oligofructose and corresponding spinning solution with without oligofructose nano fibrous membrane and
Vigor test result figure (P-F-L NF, 2.5% oligofructose/6% polyvinyl alcohol/plant of lactobacillus plantarum in corresponding spinning solution
Object lactobacillus nano fibrous membrane；P-F-L S, 2.5% oligofructose/6% polyvinyl alcohol/lactobacillus plantarum spinning solution；P-L NF,
6% polyvinyl alcohol/lactobacillus plantarum nano fibrous membrane；P-L S, 6% polyvinyl alcohol/lactobacillus plantarum spinning solution；**,P<
0.001)。
Fig. 8 is that the lactobacillus plantarum after Rhodamine 123 dyes prepares gained load oligofructose and lactobacillus plantarum
Nano fibrous membrane shows fluorescent microscopy images.
Specific implementation mode
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
Embodiment 1
Different prebiotics test the probiotic effects of probiotics：
(1) four kinds of Lactobacillus (Lactobacillus casei, L.casei are chosen；Lactobacillus paracasei, L.paracasei；It plants
Object lactobacillus, L.plantarum；Lactobacillus acidophilus, L.acidophilus) it is (green in MRS culture mediums and two kinds of Bifidobacteriums
Spring Bifidobacterium, B.adolescence；Two qi Bifidobacteriums, B.bifidum) in modified MRS culture medium (peptone 25g, ox
Meat extract 25g, yeast extract 12.5g, glucose 20g, sodium acetate 12.5g, dipotassium hydrogen phosphate 5g, ammonium citrate 5g, magnesium sulfate 0.5g,
Manganese sulfate 0.13g, L-cysteine 1.25g, Tween-80 2.5g, 1000mL water, pH 7.0) in trained under 37 DEG C of anaerobic conditions
It supports for 24 hours, then transfers and continue to activate 18h, obtain a concentration of 109The bacterium solution of CFU/mL.
(2) three kinds of oligosaccharide (oligofructose FOS, galactooligosaccharide GOS and oligoisomaltose IMO) prebiotics point are chosen
Glucose sugared (carbon source) in the MRS culture mediums and modified MRS culture medium of other alternative steps (1) prepares culture medium, and step (1) is obtained
To bacterium solution be diluted to 108Then CFU/mL is added in above-mentioned culture medium by the inoculum concentration of 1% (v/v) in 37 DEG C of anaerobism items
It is cultivated under part, measures the absorbance value (OD of different time points culture solution600), draw growth curve.Meanwhile to be free of glucose
MRS culture mediums and modified MRS culture medium as blank control.Different oligosaccharide are weighed with growth indexes (Growth GI)
Probiotic effects.
Wherein, P：Oligosaccharide；G：Glucose；C：Blank；AP24、AG24、AC24Represent the absorbance after culture for 24 hours；AP0、
AG0、AC0Represent the absorbance of 0h.
The utilization power of different three kinds of oligosaccharide of probiotics pair is as shown in figure 1 and table 1 in the present embodiment.
Table 1
By Fig. 1 and 1 result of table it is found that oligofructose (fructo-oligosaccharide, FOS) to Lactobacillus and
Bifidobacterium all has best probiotic effects, therefore following embodiment selects FOS to be used for functional nano-fiber as an example
The preparation of film.
Embodiment 2
The oligofructose (FOS) of various concentration tests the probiotic effects of probiotics：
The present embodiment difference from Example 1 be choose various concentration (0.5%, 1.0%, 1.5%, 2.0%,
2.5%, 3.0%, w/v) oligofructose replace MRS and modified MRS culture medium in glucose (carbon source), finally measure culture
Absorbance value (the OD of liquid600) and pH.Four kinds of Bacillus acidi lacticis of oligofructose additive amount pair of various concentration and two kinds of Bifidobacterium benefits
Raw effect contrast figure is as shown in Figure 2.Fig. 2 the result shows that with oligofructose additive amount increase, to the prebiotic effect of probiotics
Fruit, which is presented, first enhances the trend stablized afterwards.
Embodiment 3
A kind of preparation of the nano fibrous membrane of load FOS of the present embodiment, is as follows：
(1) polyvinyl alcohol is dissolved in the poly-vinyl alcohol solution for stirring 1h in aseptic deionized water at 80 DEG C and preparing 6wt.%,
It is cooled to room temperature.It is then respectively adding the oligofructose stirring of 0.5%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% (w/w)
It is uniformly mixed, obtains polyvinyl alcohol/oligofructose spinning solution of different oligofructose concentration.
(2) polyvinyl alcohol of gained/oligofructose spinning solution is sucked in the asepsis injector of 5mL, using the needle of 0.6mm
The distance of head, adjustment syringe needle to receiver is 14cm, and adjusting voltage is 16kV, and flow velocity 0.5mL/h, temperature is 25 DEG C, humidity
It is 40%, spinning 1h respectively obtains the nano fibrous membrane (PVA/FOS) of the load FOS of different oligofructose concentration.
The viscosity and electricity of polyvinyl alcohol/oligofructose spinning solution of different oligofructose concentration in the present embodiment step (1)
The results are shown in Table 2 for conductance.
Table 2
As seen from the results in Table 2, the addition of oligofructose is little to the viscosity influence of poly-vinyl alcohol solution, and conductivity is with oligomeric
Fructose additive amount increases and reduces.
Verify benefit of the nano fibrous membrane to different probiotics of the load FOS of different oligofructose concentration obtained by the present embodiment
It comes into force fruit, is as follows：
(1) as prepared MRS and modified MRS culture medium without glucose (carbon source) in embodiment 1；
(2) nano fibrous membrane of 20mg the present embodiment is taken to be added separately in the culture medium of 5mL steps (1), it then will be real
It applies the bacterium solution (Lactobacillus casei, lactobacillus plantarum, bifidobacterium adolescentis, two qi Bifidobacteriums) that example 1 obtains and is diluted to 108CFU/
ML is added by 1% (v/v) additive amount in culture medium, is cultivated under 37 DEG C of anaerobic conditions for 24 hours, is measured culture medium absorbance value
(OD600), while being compareed with 6% sterile nano fibrous membrane (6%PVA).
Nano fibrous membrane is to Lactobacillus casei, lactobacillus plantarum, bifidobacterium adolescentis, two qi bifid bars obtained by the present embodiment
The probiotic effects of bacterium are as shown in Table 3 and Table 4.
Table 3
Table 4
By table 3 and 4 result of table it is found that with oligofructose content in tunica fibrosa increase, tunica fibrosa is to different probiotics
Probiotic effects presentation first increase the trend stablized afterwards, therefore select 2.5% oligofructose for optimum addition.
Embodiment 4
A kind of preparation of the nano fibrous membrane of embedding probiotics of the present embodiment, is as follows：
(1) it using lactobacillus plantarum as pattern bacterium, chooses a concentration of 10 in embodiment 19The lactobacillus plantarum bacterium solution of CFU/mL
1mL is added in the 6% aseptic polyethylene alcohol spinning solution of 20mL, is stirred 10min under normal temperature condition, is obtained 6% polyvinyl alcohol/plant
Object lactobacillus spinning solution.
(2) gained polyvinyl alcohol/lactobacillus plantarum spinning solution is sucked in the asepsis injector of 5mL, using the needle of 0.6mm
The distance of head, adjustment syringe needle to receiver is 14cm, adjusts different spinning voltage (10kV, 12kV, 14kV and 16kV) respectively,
Flow velocity is 0.5mL/h, and temperature is 25 DEG C, and humidity 40%, spinning 1h obtains the nano fibrous membrane of embedded plant lactobacillus
(PVA/L.plantarum)。
The activity of lactobacillus plantarum in nano fibrous membrane under the conditions of different spinning voltages obtained by verification the present embodiment, specifically
Steps are as follows：
(1) it is prepared according to embodiment 1 and is free of glucose (carbon source) MRS culture mediums；
(2) nano fibrous membrane of 20mg the present embodiment is taken to be added separately in the culture medium of 5mL steps (1), 37 DEG C of anaerobism
Under the conditions of cultivate and measure culture medium absorbance value (OD for 24 hours600)。
The present embodiment adjust respectively spinning voltage be 10kV, 12kV, 14kV and 16kV when, gained difference spinning voltage condition
Under nano fibrous membrane in lactobacillus plantarum Activity Results it is as shown in Figure 3.By Fig. 3 results it is found that spinning voltage (10~
It does not make significant difference to the activity of lactobacillus plantarum in fiber 16kV), lactobacillus plantarum is embedded still keeps higher in the fibre
Vigor.
Embodiment 5
The preparation of a kind of load prebiotics of the present embodiment and the nano fibrous membrane of probiotics, is as follows：
(1) polyvinyl alcohol is dissolved in aseptic deionized water, obtains 6wt.% polyvinyl alcohol spinning solutions；Again to polyvinyl alcohol
The oligofructose of 2.5wt.% is added in spinning solution, stirs evenly, obtains polyvinyl alcohol/oligofructose spinning solution；
(2) it chooses a concentration of 10 in embodiment 19The lactobacillus plantarum bacterium solution (1%v/v additive amounts) of CFU/mL is added to step
Suddenly stirred evenly under normal temperature condition in polyvinyl alcohol/oligofructose spinning solution obtained by (1), obtain polyvinyl alcohol/oligofructose/
Lactobacillus plantarum spinning solution；
(3) gained polyvinyl alcohol/oligofructose/lactobacillus plantarum spinning solution is sucked in the asepsis injector of 5mL, is used
The distance of the syringe needle of 0.6mm, adjustment syringe needle to receiver is 14cm, and adjusting spinning voltage is 16kV, flow velocity 0.5mL/h, temperature
Degree is 25 DEG C, humidity 40%, and spinning 1h obtains the nano fibrous membrane (PVA/FOS/ of load oligofructose and lactobacillus plantarum
L.plantarum)。
The SEM figures and distribution of fiber diameters of the nano fibrous membrane of load oligofructose and lactobacillus plantarum obtained by the present embodiment
Figure is as shown in Figure 4.As shown in Figure 4, fibre diameter is resulted in the fibre locally become larger, pearl section occur since probiotics is embedded
Structure.
Test and characterization：
(1) examination of infrared spectrum：By 5 (PVA/FOS/ of embodiment 3 (PVA/FOS, 2.5wt.%FOS) and embodiment
L.plantarum nano fibrous membrane, pva powder (PVA), oligofructose powder (FOS) and the lactobacillus plantarum) obtained
Thalline (L.plantarum) carries out IR spectrum scanning, and scanning range is 3800~500cm-1, tunica fibrosa use ATR, latter several groups
Sample uses KRr tablettings.As a result see Fig. 5.In 1300~900cm in the absorption spectrum of lactobacillus plantarum in figure-1Section exists special
Levy peak, this is because in thalline albumen and nucleic acid characteristic absorption.5 gained nano fibrous membrane (PVA/FOS/ of same embodiment
L.plantarum in 1300~900cm in infrared spectrogram)-1It comes to a point and sends out there are characteristic absorption peak and characteristic peak in range
Raw certain offset, illustrates that lactobacillus plantarum is embedded in nanofiber.
(2) thermogravimetric analysis：Nano fibrous membrane (PVA/FOS/L.plantarum), the polyvinyl alcohol powder that embodiment 5 is obtained
Last (PVA), oligofructose powder (FOS) and lactobacillus plantarum thalline (L.plantarum) carry out thermogravimetric analysis, and temperature range is
25 DEG C~700 DEG C, thermogravimetric analysis figure is shown in Fig. 6 (A, TGA；B,DTG).The decomposition of lactobacillus plantarum is divided into three as seen from Figure 6
Stage embodies and can be found in Fig. 6 A (51 DEG C, 217 DEG C, 319 DEG C).In addition, it will be appreciated from fig. 6 that the Nanowire that embodiment 5 obtains
The thermal stability of lactobacillus plantarum increases in dimension film.
(3) nano fibrous membrane containing oligofructose and corresponding spinning solution and nano fibrous membrane without oligofructose and right
The vigor of lactobacillus plantarum in spinning solution is answered to test：It is prepared according to embodiment 1 and is free of glucose (carbon source) MRS culture mediums；It takes
The nano fibrous membrane of 20mg embodiments 3 and embodiment 5 is added separately in the above-mentioned culture mediums of 5mL, equally, takes 600 μ L couple respectively
The spinning solution answered is added separately in 5mL culture mediums, and measurement culture medium absorbance value for 24 hours is cultivated under 37 DEG C of anaerobic conditions
(OD600).The results are shown in Figure 7.As shown in Figure 7, lactobacillus plantarum in nanofiber or spinning solution containing oligofructose
Vigor is significantly higher than the lactobacillus plantarum (P in the fiber or spinning solution without oligofructose<0.001).
(4) existence of verification lactobacillus plantarum in the fibre：With gained load oligofructose and plant in embodiment 5
The nano fibrous membrane of lactobacillus is the difference is that the lactobacillus plantarum being added is dyed by Rhodamine 123.Then by causing
The presence in the fibre of fluorescence microscope lactobacillus plantarum and distribution situation.The shows fluorescent microscopy images of gained nano fibrous membrane
As shown in Figure 8.As seen from Figure 8, the lactobacillus plantarum of fluoresced green is uniformly distributed in the fibre.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Claims (10)
1. the preparation method of a kind of load prebiotics and/or the nano fibrous membrane of probiotics, it is characterised in that include the following steps：
(1) polyvinyl alcohol is dissolved in aseptic deionized water, obtains polyvinyl alcohol spinning solution；Add again into polyvinyl alcohol spinning solution
Enter prebiotics, stir evenly, obtains polyvinyl alcohol/prebiotics spinning solution；
(2) in polyvinyl alcohol spinning solution or polyvinyl alcohol/the prebiotics spinning solution probiotics bacterial liquid being added in step (1),
It stirs evenly, obtains polyvinyl alcohol/probiotics spinning solution or polyvinyl alcohol/prebiotics/probiotics spinning solution；
(3) load prebiotics and/or probiotics are prepared by electrostatic spinning in spinning solution obtained by step (1) or step (2)
Nano fibrous membrane.
2. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is：Aseptic deionized water described in step (1) refers to the deionization after 115~121 DEG C of 15~20min of high-temperature sterilization
Water.
3. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is：The mass concentration of polyvinyl alcohol spinning solution described in step (1) is 6%~10%.
4. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is：Prebiotics described in step (1) include at least one of oligosaccharide, polysaccharide, flavones, polyphenol, the prebiotics
Additive amount be polyvinyl alcohol/prebiotics spinning solution quality 0.5%~3%.
5. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is：Probiotics described in step (2) refers at least one of Lactobacillus and Bifidobacterium；The probiotics
A concentration of the 10 of bacterium solution8~1010CFU/mL。
6. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is：The addition of probiotics bacterial liquid described in step (2) and polyvinyl alcohol spinning solution or polyvinyl alcohol/prebiotics spinning solution
Volume ratio be 1:20.
7. the preparation method of a kind of load prebiotics according to claim 1 and/or the nano fibrous membrane of probiotics, special
Sign is that the condition of electrostatic spinning described in step (3) is：Spinning flow velocity be 0.2~1.0mL/h, needle diameter 0.4~
0.6mm, voltage are 10~20kV, and it is 8~14cm to receive distance, and temperature is 20~25 DEG C, and humidity is 35%~55%.
8. a kind of load prebiotics and/or the nano fibrous membrane of probiotics, it is characterised in that：Pass through any one of claim 1~7
The method is prepared.
9. a kind of load prebiotics according to claim 8 and/or the nano fibrous membrane of probiotics, it is characterised in that：Institute
The fibre diameter for stating load prebiotics and/or the nano fibrous membrane of probiotics is 210~1040nm.
10. the nano fibrous membrane of a kind of load prebiotics and/or probiotics described in claim 8 or 9 is in functional food
Application.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110934894A (en) * | 2019-12-09 | 2020-03-31 | 西南交通大学 | Bifidobacterium bifidum-loaded anti-tumor fiber local implantation agent and preparation method and application thereof |
| WO2020097062A1 (en) | 2018-11-05 | 2020-05-14 | University Of Louisville Research Foundation | Methods and composition for treating microbial infections |
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| CN109620797A (en) * | 2019-01-24 | 2019-04-16 | 东南大学 | A kind of nanometer fiber slow-releasing solid formulation and preparation method thereof of tolerance gastric juice stress |
| CN109620797B (en) * | 2019-01-24 | 2023-02-03 | 东南大学 | Gastric juice stress-resistant nanofiber sustained-release solid agent and preparation method thereof |
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| CN110934894B (en) * | 2019-12-09 | 2021-11-30 | 西南交通大学 | Bifidobacterium bifidum-loaded anti-tumor fiber local implantation agent and preparation method and application thereof |
| CN112695386A (en) * | 2020-12-16 | 2021-04-23 | 义乌禾维科技有限公司 | Composite fiber containing antioxidant active probiotics and preparation method thereof |
| CN114287634A (en) * | 2022-01-07 | 2022-04-08 | 东北农业大学 | Probiotic-embedded Arabic gum composite fiber/capsule as well as preparation method and application thereof |
| CN114525221A (en) * | 2022-02-21 | 2022-05-24 | 华南理工大学 | Method for improving tolerance of probiotics and application thereof |
| CN114525221B (en) * | 2022-02-21 | 2023-11-03 | 华南理工大学 | Method for improving tolerance of probiotics and application thereof |
| CN115305653A (en) * | 2022-08-31 | 2022-11-08 | 江苏大学 | Preparation method and application of food antibacterial nanofiber membrane |
| CN115305653B (en) * | 2022-08-31 | 2024-03-19 | 江苏大学 | Preparation method and application of food antibacterial nanofiber membrane |
| CN116084047A (en) * | 2022-12-20 | 2023-05-09 | 大连工业大学 | Functional nanofiber co-loaded with probiotics and antioxidants |
| CN117107395A (en) * | 2023-08-22 | 2023-11-24 | 东北农业大学 | Preparation method of functional nanofiber carrying probiotics altogether |
| CN117107395B (en) * | 2023-08-22 | 2024-03-22 | 东北农业大学 | Preparation method of functional nanofiber carrying probiotics altogether |
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