CN108586043A - A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock - Google Patents
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock Download PDFInfo
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- CN108586043A CN108586043A CN201810509853.6A CN201810509853A CN108586043A CN 108586043 A CN108586043 A CN 108586043A CN 201810509853 A CN201810509853 A CN 201810509853A CN 108586043 A CN108586043 A CN 108586043A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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Abstract
The present invention relates to a kind of methods producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, include the following steps：（1）Silicate bacteria is cultivated；（2）The culture of fixed nitrogen phosphate solubilizing bacteria；（3）Silicate bacteria secretes silica and aluminium oxide in organic acid abjection phosphorus ore；（4）Fixed nitrogen phosphate solubilizing bacteria secretion organic acid dissolves the phosphorus in low-grade phosphate ore, by step（3）Gained filtrate and step（4）The mixing of gained bacterium solution is composite fertilizer, the method of the present invention phosphorus removal efficiency is high, the mineral element of the easy absorptions such as easy silicon, calcium, the iron absorbed is not only contained containing a great number of elements nitrogen phosphorus, also in the composite fertilizer being prepared into, and active microbial content is abundant, there are significant effect of increasing production, and the low-grade phosphate ore resource for adequately utilizing China abundant.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of to be given birth to using microorganism species dissolving mid low grade phosphate rock
The method for producing composite fertilizer.
Background technology
Phosphorus is one of most important nutrient of plant, and for a long time, phosphate fertilizer plays in terms of improving crop yield
Important role.The production of traditional phosphate fertilizer be usually using the inorganic acid solutions ground phosphate rock such as sulfuric acid, by sulfuric acid to ground phosphate rock into
Row chemistry ferments.But the phosphate fertilizer utilization efficiency of this method production is very low, about 80% or more P elements are not made
Object absorbs, but by soil fixing, therefore cause the significant wastage of resource and be produced into the raising of wood, while also resulting in huge
Big environmental pollution.
China is a phosphate rock resource big country, but most of is all low-grade phosphate ore, and the content of phosphorus pentoxide is 20%
Hereinafter, directly application only shows certain effect on acid soil and certain crops, and in ore Phosphate minerals it is embedding
Cloth granularity, complexity and grade are related.Currently, due to carrying out ore dressing and Beneficiation into high concentration phosphorus to these low-grade phosphate ores
The cost of fertilizer is very high, and many low-grade phosphate ore resources are all discarded, and are not developed and utilized effectively.
In recent years, with the new demand of the development of biotechnology and Green Chemistry, Ore leaching technology by microbe is handling low product
More and more huge effect has been played in terms of position, difficult, complex ore.It is micro- using chemosynthetic autotrophs such as Thiobacillus ferrooxidans
Biology obtains commercial Application on the metals such as the complicated copper mine of processing, gold mine extraction copper, gold.Currently, utilizing microorganism
In terms of soaking mine technical finesse phosphorus ore production microorganism phosphate fertilizer, also there is a small amount of related invention patent both at home and abroad, but these patents are mostly
It is relatively harsh to the condition of culture requirement of microorganism using chmosynthetic heterotrophs microorganism, while also indirectly improving and being produced into
This.In addition, not having substantially particular for the patent of invention of the utilization of low-grade phosphate ore resource at present, if utilizationization can be different
The type of supporting microbiological treatment low-grade phosphate ore produces complex microorganism phosphate fertilizer, thus both can overcome the disadvantages that former related invention patent not
Foot improves p fertilizer efficiency, while the low-grade phosphate ore resource that can make China abundant again is adequately developed and utilized.
Invention content
The purpose of the present invention is to provide a kind of sides producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock
Method, this method production cost is low, nitrogen and phosphorus content is high and can improve phosphate fertilizer utilization efficiency.
To achieve the goals above, the technical scheme is that：It is a kind of to dissolve middle-low grade phosphorus using microorganism species
The method that mine produces composite fertilizer, includes the following steps：
（1）Silicate bacteria is cultivated：
（2）The culture of fixed nitrogen phosphate solubilizing bacteria：
（3）Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria；
（4）Dissolve the phosphorus in low-grade phosphate ore using the secretion of fixed nitrogen phosphate solubilizing bacteria.
The step（1）Middle silicate bacteria is cultivated：
A, silicate bacteria culture medium is prepared, the 1-2h that sterilizes at 100-150 DEG C is spare.
B, in the inoculation of medium silicate bacteria being cooled to room temperature, in 26-29 DEG C, the condition of 180-250 r/min
The exponential phase of lower culture to bacterium is spare.
Silicate bacteria culture medium is in a：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:
1000ml、pH:7.1-7.3。
Silicate bacteria inoculum concentration is the 0.8%-1.5% of culture medium quality in the b.
The step（2）The culture of middle fixed nitrogen phosphate solubilizing bacteria includes：Fixed nitrogen phosphate solubilizing bacteria is seeded to sterilized phosphorus decomposing culture
In base, 5-7 d are cultivated at 27-29 DEG C, 150-200 r/min, obtain fixed nitrogen phosphorus decomposing bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.0-
7.2。
The step（3）Silica and aluminium oxide are in the middle secretion abjection phosphorus ore using silicate bacteria：By silicon
Hydrochlorate bacteria log growth period bacterium solution, low-grade phosphate ore and silicate bacteria culture medium in mass ratio 1:（25-45）：1000 is mixed
It closes, 18-23d is cultivated at 27-29 DEG C, 150-200 r/min.
The step（4）The phosphorus that the middle secretion using fixed nitrogen phosphate solubilizing bacteria dissolves in low-grade phosphate ore is：By step（3）
150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate, fixed nitrogen phosphorus decomposing bacteria culture fluid, filter residue, phosphorus decomposing culture medium are pressed respectively
According to mass ratio 1:（20-25）: 1000 mixing cultivate 10-15d, with step at 28 DEG C, 150-200 r/min（3）Gained filtrate
It mixes up to the composite fertilizer.
The principle of the invention：
Silicate bacteria refers mainly to gel-shaped bacillus, and of less demanding to nutritional condition, environmental condition is adaptable.Silicic acid
Salt mineral can promote organic acids, amino acid and the polysaccharide such as silicate bacteria production oxalic acid, tartaric acid, malic acid, while can stimulate silicon
Hydrochlorate bacterial metabolism generates specific citric acid and amino acid；These metabolites make silicate mineral with good decomposition
With.
Fixed nitrogen phosphate solubilizing bacteria can secrete different types of organic acid, organized enzyme in growth, these organic acids can reduce pH
Value acts synergistically with organized enzyme, the phosphate of slightly solubility is made to dissolve.
Beneficial effects of the present invention：
1, the present invention first uses the silicon and aluminium in silicate bacteria dissolving low-grade phosphate ore, destroys phosphorus ore structure, reuses fixed nitrogen
Phosphate solubilizing bacteria dissolves out the phosphorus in phosphorus ore, and the dissolution rate of phosphorus is made to improve.And it is thin that fertilizer produced by the present invention contains abundant silicate
Bacterium, fixed nitrogen phosphate solubilizing bacteria can be such that the available phosphorus content in soil increases using the activity of these microorganisms, and phosphate fertilizer utilization efficiency improves,
And increases Soil available nitrogen content, can be that soil supplements a variety of mineral elements such as silicon, calcium, improve biological activity of soil, promote
The formation fallen into Soil Probiotics, there is significant effect of increasing production.
2, the phosphate fertilizer of this method production is suitable for various soil and crops, without phosphorus residual.
3, this method more can adequately utilize the abundant low-grade phosphate ore resource in China, and phosphorus dissolution rate is high in phosphorus ore, together
When reduce production of phosphate fertilizer cost, have important practical significance to China's agricultural development and Phosphate Fertilizer Industry environmental improvement.
Specific implementation mode
With reference to specific embodiment, the present invention is described in further detail.Silicate bacteria used is thought again by Xingtai
Special bio tech ltd provides, and fixed nitrogen phosphate solubilizing bacteria is provided by Shandong Su Kehan bioengineering limited liability company.Phosphorus used
Mine experiment phosphorus ore sample used is derived from Zhongxiang Hubei, and phosphorus ore grade is 18.25%, Al2O3Content 2.19%, SiO2Content 31.69%,
Belong to low-grade phosphate ore.
Embodiment 1
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, it is characterised in that it includes following step
Suddenly：
（1）Silicate bacteria is cultivated；
A, according to formula：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:1000ml、pH:7.1, configuration culture
Base, the 2h that sterilizes at 150 DEG C is spare,
B, in the inoculation of medium silicate bacteria being cooled to room temperature, inoculum concentration is the 1.5% of culture medium quality, 29 DEG C,
The exponential phase of culture to bacterium is spare under conditions of 250 r/min.
（2）The culture of fixed nitrogen phosphate solubilizing bacteria；
Fixed nitrogen phosphate solubilizing bacteria is seeded in sterilized phosphorus decomposing culture medium, 7 d is cultivated at 29 DEG C, 200 r/min, obtains fixed nitrogen
Phosphorus decomposing bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.2.
（3）Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria：
Mid low grade phosphate rock is processed as 100 mesh powders, in mass ratio silicate bacteria exponential phase bacterium solution: low-grade phosphorus
Miberal powder end: silicate bacteria culture medium=1: 45：1000 mixing, 23d is cultivated at 29 DEG C, 200 r/min.
（4）Utilize the phosphorus in the secretion dissolving low-grade phosphate ore of fixed nitrogen phosphate solubilizing bacteria：
By step（3）150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate respectively, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter
Slag, phosphorus decomposing culture medium are mixed according to mass ratio 1: 25: 1000,15d are cultivated at 28 DEG C, 200 r/min, with step（3）Gained
Filtrate mixes up to the composite fertilizer.
Embodiment 2
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, it is characterised in that it includes following step
Suddenly：
（1）Silicate bacteria is cultivated：
A, according to formula：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:1000ml、pH:7.1, configuration culture
Base, the 1h that sterilizes at 100 DEG C is spare,
B, in the inoculation of medium silicate bacteria being cooled to room temperature, inoculum concentration is the 0.8% of culture medium quality, 26 DEG C,
The exponential phase of culture to bacterium is spare under conditions of 180 r/min.
（2）The culture of fixed nitrogen phosphate solubilizing bacteria；
Fixed nitrogen phosphate solubilizing bacteria is seeded in sterilized phosphorus decomposing culture medium, 5d is cultivated at 27 DEG C, 150r/min, obtains fixed nitrogen solution
Phosphorus bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.0.
（3）Utilize the phosphorus in the secretion dissolving low-grade phosphate ore of fixed nitrogen phosphate solubilizing bacteria：
Mid low grade phosphate rock is processed as to 80 powder, in mass ratio silicate bacteria exponential phase bacterium solution: low-grade phosphate ore
Powder: silicate bacteria culture medium=1: 25：1000 mixing, 18d is cultivated at 27 DEG C, 150 r/min.
（4）Utilize the phosphorus in the secretion dissolving low-grade phosphate ore of fixed nitrogen phosphate solubilizing bacteria：
By step（3）150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate respectively, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter
Slag, phosphorus decomposing culture medium are mixed according to mass ratio 1: 20: 1000,10d are cultivated at 28 DEG C, 150r/min, with step（3）Gained is filtered
Liquid mixes up to the composite fertilizer.
Embodiment 3
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, it is characterised in that it includes following step
Suddenly：
（1）Silicate bacteria is cultivated：
A, according to formula：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:1000ml、pH:7.1, configuration culture
Base, the 1.5h that sterilizes at 100 DEG C is spare,
B, in the inoculation of medium silicate bacteria being cooled to room temperature, inoculum concentration is the 1% of culture medium quality, 28 DEG C, 190
The exponential phase of culture to bacterium is spare under conditions of r/min.
（2）The culture of fixed nitrogen phosphate solubilizing bacteria：
Fixed nitrogen phosphate solubilizing bacteria is seeded in sterilized phosphorus decomposing culture medium, 6d is cultivated at 28 DEG C, 180r/min, obtains fixed nitrogen solution
Phosphorus bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.0.
（3）Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria：
Mid low grade phosphate rock is processed as 90 mesh powders, in mass ratio silicate bacteria exponential phase bacterium solution: low-grade phosphorus
Miberal powder end: silicate bacteria culture medium=1: 30：1000 mixing, 19d is cultivated at 28 DEG C, 180 r/min.
（4）Utilize the phosphorus in the secretion dissolving low-grade phosphate ore of fixed nitrogen phosphate solubilizing bacteria：
By step（3）150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate respectively, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter
Slag, phosphorus decomposing culture medium are mixed according to mass ratio 1: 23: 1000,13d are cultivated at 28 DEG C, 180 r/min, with step（3）Gained
Filtrate mixes up to the composite fertilizer.
Embodiment 4
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, it is characterised in that it includes following step
Suddenly：
（1）Silicate bacteria is cultivated：
A, according to formula：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:1000ml、pH:7.2, configuration culture
Base, the 1.8h that sterilizes at 140 DEG C is spare,
B, in the inoculation of medium silicate bacteria being cooled to room temperature, inoculum concentration is the 0.9% of culture medium quality, 27 DEG C,
The exponential phase of culture to bacterium is spare under conditions of 240 r/min.
（2）The culture of fixed nitrogen phosphate solubilizing bacteria：
Fixed nitrogen phosphate solubilizing bacteria is seeded in sterilized phosphorus decomposing culture medium, 6d is cultivated at 28 DEG C, 180r/min, obtains fixed nitrogen solution
Phosphorus bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.2.
（3）Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria：
Mid low grade phosphate rock is processed as 90 mesh powders, in mass ratio silicate bacteria exponential phase bacterium solution: low-grade phosphorus
Miberal powder end: silicate bacteria culture medium=1: 35：1000 mixing, 18-23d is cultivated at 28 DEG C, 180 r/min.
（4）Utilize the phosphorus in the secretion dissolving low-grade phosphate ore of fixed nitrogen phosphate solubilizing bacteria：
By step（3）150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate respectively, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter
Slag, phosphorus decomposing culture medium are mixed according to mass ratio 1: 21: 1000,14d are cultivated at 28 DEG C, 180 r/min, with step（3）Gained
Filtrate mixes up to the composite fertilizer.
Embodiment 5
A method of producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, it is characterised in that it includes following step
Suddenly：
（1）Silicate bacteria is cultivated：
A, according to formula：Beef extract:3.0g, peptone:10.0g、NaCl:5.0g, water:1000ml、pH:7.3, configuration culture
Base, the 1-2h that sterilizes at 100 DEG C is spare,
B, in the inoculation of medium silicate bacteria being cooled to room temperature, inoculum concentration is the 1.2% of culture medium quality, 28 DEG C,
The exponential phase of culture to bacterium is spare under conditions of 230 r/min.
（2）The culture of fixed nitrogen phosphate solubilizing bacteria：
Fixed nitrogen phosphate solubilizing bacteria is seeded in sterilized phosphorus decomposing culture medium, 5d is cultivated at 29 DEG C, 150r/min, obtains fixed nitrogen solution
Phosphorus bacteria culture fluid, it is spare.
The phosphorus decomposing culture medium prescription is：NaCl0.5g；KCl0.2g；MgSO4·7H2O0.1g；Na2HPO4.7H2O23g；
(NH4)2SO40.1g；FeSO40.004g；MnSO40.004gL；Sucrose 10g；Yeast extract 0.5g；Distilled water 1000mL；pH7.2.
（3）Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria：
Mid low grade phosphate rock is processed as 100 mesh powders, in mass ratio silicate bacteria exponential phase bacterium solution: low-grade phosphorus
Miberal powder end: silicate bacteria culture medium=1: 44：1000 mixing, 23d is cultivated at 29 DEG C, 200 r/min.
（4）Dissolve the phosphorus in low-grade phosphate ore using the secretion of fixed nitrogen phosphate solubilizing bacteria：
By step（3）150 mesh filter-cloth filterings of gained bacterium solution collect filter residue filtrate respectively, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter
Slag, phosphorus decomposing culture medium are mixed according to mass ratio 1: 23: 1000,11d are cultivated at 28 DEG C, 200 r/min, with step（3）Gained
Filtrate mixes up to the composite fertilizer.
The Soluble phosphorus experiment of 1 present invention of test example
By sample ore grind into powder, is sampled after dry and use DlMAX-3A type X ray powder diffraction instrument assaying phase compositions.Inspection
It surveys result and shows that phosphorus ore main component is P2O518.25%、Al2O3Content 2.19%, SiO2Content 31.69%, CaO26.85%, other
21.02%。
Using embodiment 1-3 as test group 1-3,；Control group, according to steps are as follows step operation,（1）Cultivate fixed nitrogen phosphorus decomposing
Bacterium,（2）Dissolve the phosphorus in low-grade phosphate ore, the same embodiment of implementation using the secretion of fixed nitrogen phosphate solubilizing bacteria（3）, detection training
Phosphorus content after supporting in solution.
Dissolving phosphorus measurement method be：10ml culture solutions are taken to filter, after centrifugation（4000rpm, 20min）Using vanadium molybdenum Huang ratio
Color method measures culture solution P2O5Concentration calculates in culture solution according to volume multiple and dissolves phosphorus content.
Leaching rate calculation formula：Dissolve phosphorus/mineral used phosphorus content × 100%=leaching rate
Test result：
| Control group | Test group 1 | Test group 2 | Test group 3 | |
| Leaching rate | 26.74% | 51.23% | 60.58% | 63.29% |
As can be seen that effect of solubilizing phosphate of the present invention is excellent, and the control group of relative usage single culture Soluble phosphorus, test group significant effect.
Composite fertilizer's simulation experiments prepared by 2 present invention of test example
In Guizhou Province south Pingtang County tuber of pinellia planting base, the tuber of pinellia planting site for selecting continuous cropping soil physicochemical property difference little, point
For control group and test group, control group uses nitrogen, phosphorus and potassium fertilizer, test group to be used using the product of the present invention of embodiment 3 according to a conventional method
Amount is 200kg/ mus, and potash fertilizer usage amount is as control group, and two groups are unified farming, poured, and way to manage is consistent, record tuber of pinellia life
Long character, the yield and content of drug effect components for measuring the harvest tuber of pinellia.
It selects 200 plants of tuber of pinellia at random respectively in reference group, control group, such as following table is measured to growth character：
| Group | Reference group | Test group |
| Average plant height（cm） | 18.65 | 24.18 |
| Average bulbil number（It is a） | 6.8 | 9.3 |
| Harvest fresh weight（Kg/ mus） | 1108 | 1563 |
It can be seen from table using the present invention after, test group be averaged plant height, bulbil number, harvest yield dramatically increase.
Claims (6)
1. a kind of method producing composite fertilizer using microorganism species dissolving mid low grade phosphate rock, which is characterized in that including as follows
Step：
Silicate bacteria is cultivated；
The culture of fixed nitrogen phosphate solubilizing bacteria；
Utilize silica and aluminium oxide in the secretion abjection phosphorus ore of silicate bacteria；
Dissolve the phosphorus in low-grade phosphate ore using the secretion of fixed nitrogen phosphate solubilizing bacteria.
2. utilizing the method for microorganism species dissolving mid low grade phosphate rock production composite fertilizer, feature as described in claim 1
It is, the step（1）Middle silicate bacteria is cultivated：
Silicate bacteria culture medium is prepared, the 1-2h that sterilizes at 100-150 DEG C is spare,
B, it in the inoculation of medium silicate bacteria being cooled to room temperature, is trained under conditions of 26-29 DEG C, 180-250 r/min
The exponential phase supported to bacterium is spare.
3. utilizing the method for microorganism species dissolving mid low grade phosphate rock production composite fertilizer, feature as claimed in claim 2
It is：The silicate bacteria inoculum concentration is the 0.8%-1.5% of culture medium quality.
4. utilizing the method for microorganism species dissolving mid low grade phosphate rock production composite fertilizer, feature as described in claim 1
It is, the step（2）The culture of middle fixed nitrogen phosphate solubilizing bacteria includes：Fixed nitrogen phosphate solubilizing bacteria is seeded to sterilized phosphorus decomposing culture medium
In, 5-7 d are cultivated at 27-29 DEG C, 150-200 r/min, obtain fixed nitrogen phosphorus decomposing bacteria culture fluid, it is spare.
5. utilizing the method for microorganism species dissolving mid low grade phosphate rock production composite fertilizer, feature as described in claim 1
It is, the step（3）Silica and aluminium oxide include in the middle secretion abjection phosphorus ore using silicate bacteria：By silicic acid
Salt bacteria log growth period bacterium solution, low-grade phosphate ore and silicate bacteria culture medium in mass ratio 1:（25-45）：1000 mixing,
18-23d is cultivated at 27-29 DEG C, 150-200 r/min.
6. utilizing the method for microorganism species dissolving mid low grade phosphate rock production composite fertilizer, feature as described in claim 1
It is, the step（4）It is middle to dissolve the phosphorus in low-grade phosphate ore using fixed nitrogen phosphate solubilizing bacteria secretion and include：By step（3）Institute
150 mesh filter-cloth filterings of bacterium solution, respectively collection filter residue filtrate, by fixed nitrogen phosphorus decomposing bacteria culture fluid, filter residue, phosphorus decomposing culture medium according to
Mass ratio 1:（20-25）: 10-15d is cultivated in 1000 mixing at 28 DEG C, 150-200 r/min.
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| CN111440034A (en) * | 2020-04-15 | 2020-07-24 | 山东省农业科学院农业资源与环境研究所 | Soil conditioner and preparation method and application thereof |
| CN112280563A (en) * | 2020-10-28 | 2021-01-29 | 福建龙净环保股份有限公司 | Phosphate solubilizing bacteria mineralization conditioner, preparation method thereof and heavy metal contaminated soil remediation and improvement method |
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| CN112280563B (en) * | 2020-10-28 | 2022-04-26 | 福建龙净环保股份有限公司 | Phosphate solubilizing bacteria mineralization conditioner, preparation method thereof and heavy metal contaminated soil remediation and improvement method |
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Application publication date: 20180928 |