CN108578770A - A kind of injection medical and beauty treatment suspension and preparation method with long-acting function - Google Patents
A kind of injection medical and beauty treatment suspension and preparation method with long-acting function Download PDFInfo
- Publication number
- CN108578770A CN108578770A CN201810378205.1A CN201810378205A CN108578770A CN 108578770 A CN108578770 A CN 108578770A CN 201810378205 A CN201810378205 A CN 201810378205A CN 108578770 A CN108578770 A CN 108578770A
- Authority
- CN
- China
- Prior art keywords
- weight
- long
- injection
- parts
- beauty treatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/06—Flowable or injectable implant compositions
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Transplantation (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of injection medical and beauty treatment suspension and preparation method with long-acting function.It is made by following steps：A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, nano-scale fiber is made in freeze grinding；B, nano-scale fiber is added in the alcoholic solution of polyethyleneimine dendrimers, mixed liquor is made in ultrasonic disperse；C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into mixed liquor, ultrasonic disperse is uniform, and kind of the injection medical and beauty treatment suspension with long-acting function is made.Long-acting in body of the present invention by improving Sodium Hyaluronate, with good biocompatibility, the filling effect of stabilization and minimum bio-toxicity, inject has microswelling under humoral effect after body, filling effect is apparent, and even injection, effectively prevent injecting it is uneven, can be widely used for augmentation rhinoplasty injection, smoothing wrinkle injection in.
Description
Technical field
The present invention relates to medical material fields, and in particular to the preparation of beauty suspension having length more particularly, to one kind
Imitate the injection medical and beauty treatment suspension and preparation method of function.
Background technology
As people are one kind of non-operating shaping beauty to injection beauty, using the method for injection by biomaterial or people
Work synthesising biological compatibility material is injected into skin corium or subcutaneous, is reached by the different mechanism of action and reduces skinfold or modeling
One major class plastic surgery procedures of type.Injection beauty implement has the advantages that without operation, painful small, few side effects, quick, market
Demand constantly expands.
As injection beautifying technique is more and more flourishing, injection beauty crowd also increasingly grows.The injection developed at present is beautiful
Capacity materials kind is more and more.Wherein, premise that is safe, being persistently injection beauty consideration.Current injection material, after injection
Internal big city causes distending pain and sense of discomfort.Especially for sensitive constitution crowd, injection beauty still has certain risk.Mesh
Preceding existing collagen, sodium hyaluronate etc. are used for injection material.But it due to being easy to be absorbed by organisms and degrade, needs continuous regular
It is injected, it is not only of high cost, and also multiple injection is larger to body effect.Even if the prior art passes through to cross-linking hyaluronic acid
Improve anti-degradability, but the bio-toxicity of crosslinking agent causes it there are security risks.Therefore, beauty skill is safely and efficiently injected
The research and application of art are more and more in widespread attention.
Number of patent application 201410715492.2 discloses a kind of preparation method of novel skin filler, and this method uses
The amino acid and its derivative of low toxicity prepare cross-linking sodium hyaluronate gel as crosslinking agent；It is intrinsic DMTMM methods have been given full play to
The advantages such as reactivity is high, mild condition and processing simplicity, select the basic amino acid-in essential amino acid to rely ammonia
Acid and arginine or derivative are crosslinked with the c-terminus of hyaluronic acid sodium molecule as crosslinking agent and are reacted, then partially enclosed
The recognition site of internal hyaluronidase, has delayed the identification and degradation of enzyme, has finally improved the internal residence time, to open
Hair safety, long-acting Sodium Hyaluronate class injection cosmetic filler provide a kind of new technology, have the exploitation of preferable market
Foreground.
Number of patent application 201510512621.2 discloses a kind of sterile injection material.The material is mainly by cross-linked transparent
Matter acid gel, free hyaluronic acid, water soluble vitamin etc. are at being grouped as.The material has degradation cycle length, plasticity strong, steady
Qualitative good and pliability is high, can be used for subcutaneous tissue filling or skin repair, operation antiblocking and articular cavity lubrication etc., belongs to
Medical treatment and shaping and beauty field.
Number of patent application 201610563450.0 discloses a kind of injection molding process beauty modification anti-aging, beautifying skin, face
Portion's rejuvenation material.The invention pharmaceutical formulation includes hyaluronic acid, collagen, vitamin E and PLGA.Preparation method packet
It includes：（1）Prepare high-viscosity gel；（2）Prepare PLGA nanoparticles；（3）High-viscosity gel and PLGA nanoparticles is fully mixed
It closes；（4）Sterilization is filling.Compared with prior art, which has an advantageous effect in that：Collagen and hyaluronic acid
It is used in combination, reduces erythema, swelling, hardening or itch, muscle caused by single product collagen injections agent possibility
Bitterly, the symptoms such as nettle rash or other fash.The blood vessel distal cycle embolism that simple hyaluronic acid injections may cause is reduced,
Cutaneous necrosis, the generation of the severe complications probability such as embolism of retinal artery and heart and brain arterial embolism, product safety in utilization carry
It is high.
Number of patent application 201611075350.X discloses a kind of injectable beauty and shaping material and preparation method thereof.Tool
For body, the spherical hydroxyl that injectable beauty and shaping material includes collagen protein powder and grain size is 30 ~ 40 μm of invention offer
Base floats.The spherical hydroxylapatite powder for being 30 ~ 40 μm by using grain size, the smooth characteristic in surface so that its is right
The irritation of tissue is preferably minimized, it is most important that it can reach most natural effect after these round smooth microsphere injections,
The tissue of injection site is soft, no scleroma.And the particle size of microballoon both can guarantee that filler was easily injected by tiny syringe needle
Subcutaneously, it in turn ensures that microballoon is not swallowed by the monocyte of human body, macrophage etc., taken away, enduringly stays in therapentic part,
After injection can skeletonization, mechanical strength is higher.
It easily being absorbed or is degraded it can be seen that injection beauty treatment material in the prior art exists, effect persistence is poor, and
Poor to the bioactive materials property held, poor biocompatibility easily influences even injection at reuniting, and the crosslinking agent used exists
Certain toxicity, and then the problems such as the normal use of influence injection beauty.
Invention content
Effectively to solve above-mentioned technical problem, the present invention proposes a kind of injection medical and beauty treatment suspension with long-acting function
Liquid and preparation method, the problem of effectively can inhibiting to be absorbed degradation, and even injection, the uneven of injection is avoided, is pacified
Good perfection, effect are lasting.
The specific technical solution of the present invention is as follows：
It is a kind of with long-acting function injection medical and beauty treatment suspension preparation method, the injection medical and beauty treatment suspension be by
The alcoholic solution of Sodium Hyaluronate nano-scale fiber made from freeze grinding and polyethyleneimine dendrimers, gathers protein capsule
Ethylene glycol dimethacrylate microballoon disperses through ultrasonic wave added and is made, and specific preparation process is：
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
4 ~ 8 parts by weight of sodium, 36 ~ 48 parts by weight of Sodium Hyaluronate, 44 ~ 60 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：30 ~ 40 parts by weight of nano-scale fiber, 8 ~ 15 weight of polyethyleneimine dendrimers
Part, 45 ~ 62 parts by weight of alcohol；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
The injection medical and beauty treatment suspension with long-acting function is made；Wherein：65 ~ 80 parts by weight of mixed liquor, 15 ~ 25 weight of protein capsule
Part, 5 ~ 10 parts by weight of polymethyl methacrylate glycol ester microballoon.
Preferably, in the step a, alcohol is at least one of ethyl alcohol, propyl alcohol.
Preferably, in the step a, the power of freeze grinding instrument is 200 ~ 300W, and electromagnetic frequency is 5 ~ 20Hz, amount of grinding
For 0.5 ~ 5g/ batches.
Preferably, in the step a, the time of precooling is 3 ~ 6min.
Preferably, in the step a, the time of grinding is 1 ~ 3min.
Preferably, in the step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is 8 ~ 12.
Preferably, in the step b, ultrasonic frequency is 50 ~ 100kHz, and power density is 2 ~ 5W/cm2, jitter time
For 20 ~ 30min.
Preferably, in the step c, ultrasonic frequency is 50 ~ 100kHz, and power density is 2 ~ 5W/cm2, jitter time
For 30 ~ 50min.
In general, to improve the anti-degradability of injection material, crosslinking agent is used, bio-toxicity has larger potential wind
Danger.The present invention innovatively uses dendroid polyethyleneimine polymers to replace crosslinking agent, can also realize raising Sodium Hyaluronate
Long-acting in body.The diameter range of dendritic is between 10nm ~ 130nm, with common high molecular polymer
Difference, dendritic have the characteristics that low viscosity, highly dissoluble, miscibility and high response, at the same its volume and
Form can also be subject to the control of specificity in the synthesis process, to design with huge inner hydrophobic space, and surface
It is the dendritic of hydrophilic nmature.The present invention exactly utilizes this characteristic, and the nano-scale fiber of Sodium Hyaluronate is disperseed
In dendroid polyethyleneimine polymers liquid, surface layer there is excellent combination, internal cavities to have Sodium Hyaluronate
The enzymolysis of effect ground protection Sodium Hyaluronate, to improve the long-acting of Sodium Hyaluronate.
In addition, Sodium Hyaluronate belongs to hydrophilic molecule, the poor biocompatibility with body, it is difficult to effectively dispersion, injection
Easily reunite afterwards, influences the uniformity of injection.In consideration of it, the present invention improves life using polymethyl methacrylate glycol ester microballoon
Object compatibility and uniformity.There is polymethyl methacrylate glycol ester microballoon good biocompatibility and the filling stablized to make
With, bio-toxicity is small, and inject has microswelling under humoral effect after body, filling effect is apparent, in injection, microballoon energy
Enough effectively reduce the dispersibility of the resistance and injection when injection so that even injection effectively prevents injecting uneven.It is special
It is not suitable for augmentation rhinoplasty injection, smoothing wrinkle injection.
The above of the present invention also proposes a kind of injection medical and beauty treatment suspension with long-acting function, includes spherical structure
Dendritic, the enzymolysis of can effectively protect Sodium Hyaluronate, and with good biocompatibility and stablize fill out
Use use as, bio-toxicity is small.
Beneficial effects of the present invention are：
1. proposing injection of the polymer microballoon preparation with long-acting function that dendritic and high-biocompatibility is added
The method of medical and beauty treatment suspension.
2. the present invention is by the way that Sodium Hyaluronate to be scattered in dendroid polyethyleneimine polymers liquid, dendritic
Internal cavities be effectively protected the enzymolysis of Sodium Hyaluronate, the long-acting in body to improve Sodium Hyaluronate.
3. the present invention is by being added polymethyl methacrylate glycol ester microballoon, using its good biocompatibility, surely
Fixed filling effect and minimum bio-toxicity, inject has microswelling under humoral effect after body, filling effect is apparent,
And even injection, effectively prevent injecting it is uneven, can be widely used for augmentation rhinoplasty injection, smoothing wrinkle injection in.
Specific implementation mode
In the following, the present invention will be further described in detail by way of specific embodiments, but this should not be interpreted as to the present invention
Range be only limitted to example below.Without departing from the idea of the above method of the present invention, according to ordinary skill
The various replacements or change that knowledge and customary means are made, should be included in the scope of the present invention.
Embodiment 1
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
6 parts by weight of sodium, 42 parts by weight of Sodium Hyaluronate, 52 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：35 parts by weight of nano-scale fiber, 12 parts by weight of polyethyleneimine dendrimers, alcohol
53 parts by weight；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：72 parts by weight of mixed liquor, 20 parts by weight of protein capsule,
8 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is ethyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
10。
In step a, the power of freeze grinding instrument is 250W, and electromagnetic frequency 12Hz, amount of grinding is 3g/ batches；Step a
In, the time of precooling is 5min；In step a, the time of grinding is 2min；In step b, ultrasonic frequency 80kHz, power is close
Degree is 4W/cm2, jitter time 25min；In step c, ultrasonic frequency 80kHz, power density 4W/cm2, jitter time
For 40min.
Embodiment 2
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
4 parts by weight of sodium, 36 parts by weight of Sodium Hyaluronate, 60 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：30 parts by weight of nano-scale fiber, 8 parts by weight of polyethyleneimine dendrimers, alcohol
62 parts by weight；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：80 parts by weight of mixed liquor, 15 parts by weight of protein capsule,
5 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is propyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
8。
In step a, the power of freeze grinding instrument is 200W, and electromagnetic frequency 5Hz, amount of grinding is 0.5g/ batches；Step a
In, the time of precooling is 3min；In step a, the time of grinding is 3min；In step b, ultrasonic frequency 50kHz, power is close
Degree is 2W/cm2, jitter time 30min；In step c, ultrasonic frequency 50kHz, power density 2W/cm2, jitter time
For 50min.
Embodiment 3
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
8 parts by weight of sodium, 48 parts by weight of Sodium Hyaluronate, 44 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：40 parts by weight of nano-scale fiber, 15 parts by weight of polyethyleneimine dendrimers, alcohol
45 parts by weight；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：65 parts by weight of mixed liquor, 25 parts by weight of protein capsule,
10 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is ethyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
12。
In step a, the power of freeze grinding instrument is 300W, and electromagnetic frequency 20Hz, amount of grinding is 5g/ batches；Step a
In, the time of precooling is 6min；In step a, the time of grinding is 1min；In step b, ultrasonic frequency 100kHz, power
Density is 5W/cm2, jitter time 30min；In step c, ultrasonic frequency 100kHz, power density 5W/cm2, dispersion
Time is 30min.
Embodiment 4
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
5 parts by weight of sodium, 38 parts by weight of Sodium Hyaluronate, 57 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：32 parts by weight of nano-scale fiber, 10 parts by weight of polyethyleneimine dendrimers, alcohol
58 parts by weight；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：76 parts by weight of mixed liquor, 18 parts by weight of protein capsule,
6 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is propyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
9。
In step a, the power of freeze grinding instrument is 220W, and electromagnetic frequency 8Hz, amount of grinding is 1g/ batches；In step a,
The time of precooling is 3min；In step a, the time of grinding is 2min；In step b, ultrasonic frequency 60kHz, power density
For 3W/cm2, jitter time 28min；In step c, ultrasonic frequency 70kHz, power density 3W/cm2, jitter time is
45min。
Embodiment 5
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
7 parts by weight of sodium, 43 parts by weight of Sodium Hyaluronate, 50 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：38 parts by weight of nano-scale fiber, 12 parts by weight of polyethyleneimine dendrimers, alcohol
50 parts by weight；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：70 parts by weight of mixed liquor, 22 parts by weight of protein capsule,
8 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is ethyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
11。
In step a, the power of freeze grinding instrument is 280W, and electromagnetic frequency 15Hz, amount of grinding is 4g/ batches；Step a
In, the time of precooling is 5min；In step a, the time of grinding is 2min；In step b, ultrasonic frequency 80kHz, power is close
Degree is 4W/cm2, jitter time 28min；In step c, ultrasonic frequency 90kHz, power density 4W/cm2, jitter time
For 40min.
Comparative example 1
It is not added with dendroid polyethyleneimine polymers
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
6 parts by weight of sodium, 42 parts by weight of Sodium Hyaluronate, 52 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in alcoholic solution, and is uniformly dispersed by ultrasonic wave added, mixed liquor is made；
Wherein：35 parts by weight of nano-scale fiber, 65 parts by weight of alcohol；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
Kind of the injection medical and beauty treatment suspension with long-acting function is made；Wherein：72 parts by weight of mixed liquor, 20 parts by weight of protein capsule,
8 parts by weight of polymethyl methacrylate glycol ester microballoon.
In step a, alcohol is ethyl alcohol；In step a, the power of freeze grinding instrument is 250W, electromagnetic frequency 12Hz, amount of grinding
For 3g/ batches；In step a, the time of precooling is 5min；In step a, the time of grinding is 2min；In step b, ultrasonic wave frequency
Rate is 80kHz, power density 4W/cm2, jitter time 25min；In step c, ultrasonic frequency 80kHz, power density
For 4W/cm2, jitter time 40min.
Comparative example 2
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
6 parts by weight of sodium, 42 parts by weight of Sodium Hyaluronate, 52 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：35 parts by weight of nano-scale fiber, 12 parts by weight of polyethyleneimine dendrimers, alcohol
53 parts by weight；
C, protein capsule is added into the mixed liquor of step b, ultrasonic disperse is uniform, and kind of the injection medical treatment with long-acting function is made
Beauty suspension；Wherein：80 parts by weight of mixed liquor, 20 parts by weight of protein capsule.
In step a, alcohol is ethyl alcohol；In step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is
10。
In step a, the power of freeze grinding instrument is 250W, and electromagnetic frequency 12Hz, amount of grinding is 3g/ batches；Step a
In, the time of precooling is 5min；In step a, the time of grinding is 2min；In step b, ultrasonic frequency 80kHz, power is close
Degree is 4W/cm2, jitter time 25min；In step c, ultrasonic frequency 80kHz, power density 4W/cm2, jitter time
For 40min.
Above-described embodiment 1 ~ 5 and the injection medical and beauty treatment suspension obtained of comparative example 1 ~ 2, are tested saturating in its proteolysis assay
Skin surface situation after bright matter acid sodium quality, test injection, method or the condition for testing characterization are as follows：
Hyaluronic acid na concn：100g beauty suspension produced by the present invention is taken, the transparent of a concentration of 862U/mL of 100mL is added
Matter acid sodium enzyme solutions, are digested at 37 DEG C, using Agilent1100 type liquid chromatographic systems, using exclusion chromatography point
The quality of Sodium Hyaluronate when Ce Ding not digest 5h, 15h, 35h and 65h.
Even injection：Using 20 ~ 30 years old healthy women volunteer as subjects, by beauty produced by the present invention
Suspension is slowly injected into volunteer's inner forearm, and then whether the skin of injection site is smooth after observation 48h.
The results are shown in Table 1.
Table 1：
Claims (9)
1. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function, it is characterised in that：The injection medical treatment
Beauty suspension is that the Sodium Hyaluronate nano-scale fiber made from freeze grinding and the alcohol of polyethyleneimine dendrimers are molten
Liquid, protein capsule, polymethyl methacrylate glycol ester microballoon disperse through ultrasonic wave added and are made, and specific preparation process is：
A, the sodium hyaluronate solution containing sodium chloride is added in alcohol, forms fibrous Sodium Hyaluronate, is further added cold
Freeze in beveller, in fore-cooling room after liquid nitrogen cools down, is ground into grinding chamber, nano-scale fiber is made；Wherein：Chlorination
4 ~ 8 parts by weight of sodium, 36 ~ 48 parts by weight of Sodium Hyaluronate, 44 ~ 60 parts by weight of alcohol；
B, nano-scale fiber made from step a is added in the alcoholic solution of polyethyleneimine dendrimers, and auxiliary by ultrasound
It helps and is uniformly dispersed, mixed liquor is made；Wherein：30 ~ 40 parts by weight of nano-scale fiber, 8 ~ 15 weight of polyethyleneimine dendrimers
Part, 45 ~ 62 parts by weight of alcohol；
C, protein capsule, polymethyl methacrylate glycol ester microballoon are added into the mixed liquor of step b, ultrasonic disperse is uniform,
The injection medical and beauty treatment suspension with long-acting function is made；Wherein：65 ~ 80 parts by weight of mixed liquor, 15 ~ 25 weight of protein capsule
Part, 5 ~ 10 parts by weight of polymethyl methacrylate glycol ester microballoon.
2. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step a, alcohol is at least one of ethyl alcohol, propyl alcohol.
3. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step a, the power of freeze grinding instrument is 200 ~ 300W, and electromagnetic frequency is 5 ~ 20Hz, and amount of grinding is 0.5 ~ 5g/
Batch.
4. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step a, the time of precooling is 3 ~ 6min.
5. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step a, the time of grinding is 1 ~ 3min.
6. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step b, the repeated monomer number of the alkyl segment of polyethyleneimine dendrimers is 8 ~ 12.
7. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step b, ultrasonic frequency is 50 ~ 100kHz, and power density is 2 ~ 5W/cm2, jitter time is 20 ~ 30min.
8. a kind of preparation method of the injection medical and beauty treatment suspension with long-acting function according to claim 1, feature
It is：In the step c, ultrasonic frequency is 50 ~ 100kHz, and power density is 2 ~ 5W/cm2, jitter time is 30 ~ 50min.
9. a kind of injection medical and beauty treatment with long-acting function that any one of claim 1 ~ 8 the method is prepared suspends
Liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810378205.1A CN108578770A (en) | 2018-04-25 | 2018-04-25 | A kind of injection medical and beauty treatment suspension and preparation method with long-acting function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810378205.1A CN108578770A (en) | 2018-04-25 | 2018-04-25 | A kind of injection medical and beauty treatment suspension and preparation method with long-acting function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108578770A true CN108578770A (en) | 2018-09-28 |
Family
ID=63609137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810378205.1A Withdrawn CN108578770A (en) | 2018-04-25 | 2018-04-25 | A kind of injection medical and beauty treatment suspension and preparation method with long-acting function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108578770A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110279887A (en) * | 2019-07-18 | 2019-09-27 | 王月玲 | A kind of multipurpose photon epoxy resin and preparation method thereof |
-
2018
- 2018-04-25 CN CN201810378205.1A patent/CN108578770A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110279887A (en) * | 2019-07-18 | 2019-09-27 | 王月玲 | A kind of multipurpose photon epoxy resin and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11992580B2 (en) | Method of soft tissue augmentation | |
| CN106589424B (en) | Cross-linked hyaluronic acid gel for injection and preparation method thereof | |
| JP5642388B2 (en) | Hyaluronic acid gel graft mixture for injection | |
| Andre | Hyaluronic acid and its use as a “rejuvenation” agent in cosmetic dermatology | |
| CA2805008C (en) | Filler composition for tissue augmentation comprising a hydrogel of hyaluronic acid cross-linked with alkylene diamine | |
| KR101773989B1 (en) | Injectable sterile aqueous formulation based on crosslinked hyaluronic acid and hydroxyapatite for aesthetic use | |
| CN105473623B (en) | The method for being used to prepare the hyaluronic acid product of crosslinking | |
| JP5574083B2 (en) | Injectable hydrogel with high ability to give high persistence and volume | |
| JPH01265970A (en) | Collagen water solution or water dispersion solution including hyaluronic acid | |
| KR102527685B1 (en) | Silicone oil-in-water composition useful as injectable filler and scaffold for collagen growth | |
| CN107522881B (en) | Method for preparing single-phase modified sodium hyaluronate gel | |
| ES2732317T3 (en) | Dermocosmetic filler material and uses thereof for aesthetic purposes | |
| CN110279888A (en) | A kind of injection Ago-Gel agent and preparation method thereof | |
| JP2014510590A (en) | Composition comprising a filler product and at least one bioabsorbable and biodegradable silica-based material | |
| CN110327488B (en) | Injection filling microsphere preparation and preparation method thereof | |
| CN108578770A (en) | A kind of injection medical and beauty treatment suspension and preparation method with long-acting function | |
| CN110279887B (en) | Multipurpose photon cold gel and preparation method thereof | |
| CN110292655A (en) | A kind of injection fillers preparation of hydroxyl apatite and preparation method thereof | |
| JP2004517655A (en) | How to repair fat bodies | |
| CN109843345A (en) | Act on the new compositions of fat cell | |
| WO2021018377A1 (en) | Cosmetic composition with combined filler and skin regenerative effect | |
| KR102348467B1 (en) | A method for manufacturing a filler containing dna fraction and the filler prepared therefrom | |
| CN116251231B (en) | Cosmetic filling material for injection and preparation method thereof | |
| KR102417671B1 (en) | Hyaluronic acid-based filler composition having self-crosslinking system and method for manufacturing the same | |
| Devi et al. | Significance of hyaluronic acid in the biomedical field: A review |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180928 |
|
| WW01 | Invention patent application withdrawn after publication |