CN108576576A - A kind of preparation method of essence precursor material - Google Patents

A kind of preparation method of essence precursor material Download PDF

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Publication number
CN108576576A
CN108576576A CN201810431996.XA CN201810431996A CN108576576A CN 108576576 A CN108576576 A CN 108576576A CN 201810431996 A CN201810431996 A CN 201810431996A CN 108576576 A CN108576576 A CN 108576576A
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China
Prior art keywords
fermentation
parts
precursor material
preparation
enzymolysis
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Pending
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CN201810431996.XA
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Chinese (zh)
Inventor
曲广辉
赵建云
张甲贵
李娟�
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Liaocheng Xinhengji Biological Technology Co Ltd
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Liaocheng Xinhengji Biological Technology Co Ltd
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Priority to CN201810431996.XA priority Critical patent/CN108576576A/en
Publication of CN108576576A publication Critical patent/CN108576576A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/24Synthetic spices, flavouring agents or condiments prepared by fermentation
    • A23L27/25Dairy flavours
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/137Delbrueckii
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/21Streptococcus, lactococcus
    • A23V2400/249Thermophilus

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

A kind of preparation method of essence precursor material provided by the present invention, includes the following steps:Enzymolysis:35 45 parts of raw meat, 0.1 0.4 parts of compound protease will be rubbed, 18 29 parts of pure water is uniformly mixed, and 50 55 DEG C digest 2.5 4.5 hours;Enzymolysis completion, which is placed on 90 95 DEG C of water-baths, makes enzyme-deactivating in 10 15 minutes, obtains enzymolysis liquid;It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 37 parts of skimmed milk powders are added into jar fermenter, after stirring evenly, streptococcus thermophilus and Lactobacillus delbrueckii kind are inoculated with wherein, 40 45 DEG C of sealing and standings are fermented 13 24 hours;Obtain zymotic fluid.Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, 35 40 DEG C of stirrings are fermented 5 13 hours;After fermentation, you can directly apply to Maillard reaction;Or it puts it into 20 DEG C of freezers and preserves.Meat zymolyte is handled using microbe fermentation method, the degree of hydrolysis for improving meat protein is reached, removes the purpose of the bitter taste of enzymolysis liquid.

Description

A kind of preparation method of essence precursor material
Technical field
The invention belongs to technical field of food production, and in particular to a kind of preparation method of essence precursor material.
Background technology
Modern essence production technology mainly based on taste substance is homologous, is mutually tied using zymolysis technique with Maillard reaction technology Conjunction is prepared.Wherein meat flavor is mainly using the zymolyte of various meats as the precursor material of Maillard reaction.
Zymolysis technique principle is that the macromolecular substances such as the protein in the biocatalysis decomposition meat using enzyme are small point Sub- substance, such as some peptides and amino acid etc., these small-molecule substances have important role to the flavour of meat.Enzyme hydrolysis method With mild condition, side reaction is few, the reservation that is conducive to nutritional ingredient, production technology are easy to control, product stable in physicochemical property, production It is object more nutrition, safer, the advantages that non-environmental-pollution.
But the shortcomings that enzyme hydrolysis method, is equally fairly obvious:One is hydrolysis is not thorough, it is anti-to cannot be satisfied follow-up Mei Lade Needed for answering, need additionally to add a certain amount of amino acid, to further increase production cost;Moreover, utilizing enzymolysis legal system Obtaining meat flavor, often fragrance is insufficient, needs additional adding liquid essence, while increasing cost, also reduces the peace of product The natural sense of full property and flavor;For enzymolysis product often with bitter taste, the source of these bitter tastes is mainly the hydrophobic ammonia for digesting and generating Base acid and the polypeptide being made from it.The technology for the removal bitter taste applied now includes mainly exopeptidase method, embedding coverage method, physics and chemistry The methods of processing (separation, extraction, absorption etc.).Although these methods can remove or reduce bitter taste, technique is very multiple It is miscellaneous, and cost is higher, greatly limits production and application.
Microbial fermentation refers to utilizing microorganism, and under appropriate conditions, raw material is turned by specific metabolic pathway Turn to the process for the product that necessary for human is wanted.Application of the microbial fermentation in food has long history.Utilize microorganism Fermentation wine, sauce, fermented bean curd and other numerous food additives obtained etc. have been widely applied in people’s lives.
Invention content
The preparation method of a kind of essence precursor material provided by the present invention, using microbe fermentation method processing meat enzymolysis Object removes the purpose of the bitter taste of enzymolysis liquid to reach the degree of hydrolysis for improving meat protein.
A kind of preparation method of essence precursor material, which is characterized in that include the following steps:
Step 1:Enzymolysis:35-45 parts of raw meat, 0.1-0.4 parts of compound protease, 18-29 parts of mixing of pure water will be rubbed Uniformly, it digests 2.5-4.5 hours for 50-55 DEG C;Enzymolysis completion, which is placed on 90-95 DEG C of water-bath, makes enzyme-deactivating in 10-15 minutes, must digest Liquid;
Step 2:It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 3-7 is added into jar fermenter Part skimmed milk powder after stirring evenly, streptococcus thermophilus and Lactobacillus delbrueckii kind is inoculated with wherein, 40-45 DEG C of sealing Standing for fermentation 13-24 hours;Obtain zymotic fluid;
Step 3:Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, 35-40 DEG C is stirred Mix fermentation 5-13 hours;After fermentation, you can directly apply to Maillard reaction, or put it into -20 DEG C of freezers and preserve.
Preferably, in the step 2 streptococcus thermophilus and Lactobacillus delbrueckii kind with 1:1.8-1:1.5 ratio Wherein, inoculum concentration is respectively 10 for inoculation7-108A/g raw meats.
Preferably, in the step 2 streptococcus thermophilus and Lactobacillus delbrueckii kind with 1:1.7 ratio is inoculated with it In.
Preferably, saccharomycete inoculum concentration is 10 in the step 36-107A/g raw meats.
Preferably, the streptococcus thermophilus:Lactobacillus delbrueckii kind:Saccharomycete is with 15:25.5:2 ratios connect Kind is wherein.
Preferably, the raw meat is chicken, pork, beef, the flesh of fish.
The compound protease believes (China) Bioisystech Co., Ltd purchased from Novi;The strains of streptococcus thermophilus is thermophilic Hot streptococcus ATCC19258;The Lactobacillus delbrueckii kind bacterial strain is Lactobacillus delbrueckii kind CICC6045.
Beneficial effects of the present invention:It is streptococcus thermophilus, Lactobacillus delbrueckii guarantor to prepare the leavening used in essence precursor material Add three kinds of microorganisms of Leah kind and saccharomycete, the degree of hydrolysis of meat proteins can be improved, the amino acid in increasing enzymolysis liquid Also main presentation substance-bitter peptides of bitter taste have been decomposed while content, to reduce so that eliminating bitter taste;Enzyme after fermentation The content of amino acid has met needed for follow-up Maillard reaction in solution liquid, is not required to addition exogenous amino acid, greatly reduces into This;The features such as meat flavoring prepared by meat zymolyte by fermentation process has fragrance simple and honest, and fragrance remaining time is long, be not required into Addition " head is fragrant " process, this improvement eliminate the use of liquid formulation essence, not only reduce into row tradition essence production This, and considerably increase safety and the natural sense of essence.Streptococcus thermophilus, Lactobacillus delbrueckii kind and yeast Bacterium is carrying out the secondary metabolites such as vitamin, the bacteriocin of secondary metabolism generation, has very high health value, and can Inhibit the growth and breeding of other microorganisms at some extent as preservative.
Specific implementation mode
Embodiment 1
A kind of preparation method of essence precursor material, includes the following steps:
Step 1:Enzymolysis:By 35 parts of minced beef meat, 0.2 part of compound protease, 20 parts of pure water is uniformly mixed, 54 DEG C of enzymolysis 3 hours;Enzymolysis completion, which is placed on 95 DEG C of water-baths, makes enzyme-deactivating in 10 minutes, obtains enzymolysis liquid;
Step 2:It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 5 parts are added into jar fermenter Skimmed milk powder, after stirring evenly, by streptococcus thermophilus and Lactobacillus delbrueckii kind with 1:1.5 ratio is inoculated with wherein, Inoculum concentration is respectively 107-108A/g beef, 42 DEG C of sealing and standings are fermented 15 hours;Obtain zymotic fluid.
Step 3:Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, inoculum concentration are 106-107A/g raw meats, 37 DEG C of stirrings are fermented 8 hours.After fermentation, you can directly apply to Maillard reaction, or will It is put into -20 DEG C of freezers and preserves.
Embodiment 2
A kind of preparation method of essence precursor material, which is characterized in that include the following steps:
Step 1:Enzymolysis:40 parts of chicken, 0.1 part of compound protease will be rubbed, 29 parts of pure water is uniformly mixed, 50 DEG C of enzymolysis 2.5 hour;Enzymolysis completion, which is placed on 90 DEG C of water-baths, makes enzyme-deactivating in 10 minutes, obtains enzymolysis liquid;
Step 2:It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 3 parts are added into jar fermenter Skimmed milk powder, after stirring evenly, by streptococcus thermophilus and Lactobacillus delbrueckii kind with 1:1.7 ratio is inoculated with wherein, Inoculum concentration is respectively 107-108A/g raw meats, 45 DEG C of sealing and standings are fermented 13 hours;Obtain zymotic fluid.
Step 3:Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, inoculum concentration are 106-107A/g raw meats, 35 DEG C of stirrings are fermented 5 hours;After fermentation, you can directly apply to Maillard reaction, or will It is put into -20 DEG C of freezers and preserves.
Embodiment 3
Step 1:Enzymolysis:By 45 parts of minced pork meat, 0.4 part of compound protease, 25 parts of pure water is uniformly mixed, 55 DEG C of enzymolysis 4.5 hour;Enzymolysis completion, which is placed on 92 DEG C, makes enzyme-deactivating in 13 minutes, obtains enzymolysis liquid;
Step 2:It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 7 parts are added into jar fermenter Skimmed milk powder, after stirring evenly, by streptococcus thermophilus and Lactobacillus delbrueckii kind with 1:1.8 ratio is inoculated with wherein, Inoculum concentration is respectively 107-108A/g raw meats, 40 DEG C of sealing and standings are fermented 24 hours;Obtain zymotic fluid.
Step 3:Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, inoculum concentration are 106-107A/g raw meats, 40 DEG C of stirrings are fermented 13 hours;After fermentation, you can Maillard reaction is directly applied to, or - 20 DEG C of freezers are put it into preserve.

Claims (6)

1. a kind of preparation method of essence precursor material, which is characterized in that include the following steps:
Step 1:Enzymolysis:35-45 parts of raw meat, 0.1-0.4 parts of compound protease will be rubbed, 18-29 parts of pure water is uniformly mixed, 50-55 DEG C digests 2.5-4.5 hours;Enzymolysis completion, which is placed on 90-95 DEG C of water-bath, makes enzyme-deactivating in 10-15 minutes, obtains enzymolysis liquid;
Step 2:It ferments for the first time:Step 1 gained enzymolysis liquid is all added in jar fermenter, 3-7 parts are added into jar fermenter and takes off Fat milk powder after stirring evenly, streptococcus thermophilus and Lactobacillus delbrueckii kind is inoculated with wherein, 40-45 DEG C of sealing and standing Fermentation 13-24 hours;Obtain zymotic fluid;
Step 3:Second of fermentation:Step 2 gained zymotic fluid is transferred in fermentation tank, and inoculation yeast bacterium, 35-40 DEG C of stirring hair Ferment 5-13 hours;After fermentation, you can directly apply to Maillard reaction;Or it puts it into -20 DEG C of freezers and preserves.
2. a kind of preparation method of essence precursor material according to claim 1, which is characterized in that thermophilic in the step 2 Streptococcus and Lactobacillus delbrueckii kind are with 1:1.8-1:Wherein, inoculum concentration is respectively 10 for 1.5 ratio inoculation7-108A/g Raw meat.
3. a kind of preparation method of essence precursor material according to claim 2, which is characterized in that the streptococcus thermophilus With Lactobacillus delbrueckii kind with 1:1.7 ratio inoculation.
4. a kind of preparation method of essence precursor material according to claim 1, which is characterized in that yeast in the step 3 Bacterium inoculum concentration is 106-107A/g raw meats.
5. a kind of preparation method of essence precursor material according to claim 1, which is characterized in that preferably, described is thermophilic Hot streptococcus:Lactobacillus delbrueckii kind:Saccharomycete is with 15:25.5:2 ratios are inoculated with.
6. a kind of preparation method of essence precursor material according to claim 1, which is characterized in that the raw meat is chicken Meat, pork, beef, the flesh of fish.
CN201810431996.XA 2018-05-08 2018-05-08 A kind of preparation method of essence precursor material Pending CN108576576A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114847413A (en) * 2022-05-31 2022-08-05 西南民族大学 Yak meat source phagostimulant, preparation method and application thereof in pet food additive
CN118303607A (en) * 2024-06-07 2024-07-09 烟台金丝猴食品科技有限公司 Essence composition, preparation method thereof and toffee using essence composition

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114847413A (en) * 2022-05-31 2022-08-05 西南民族大学 Yak meat source phagostimulant, preparation method and application thereof in pet food additive
CN118303607A (en) * 2024-06-07 2024-07-09 烟台金丝猴食品科技有限公司 Essence composition, preparation method thereof and toffee using essence composition

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