CN108572172A - Detection device and detection method - Google Patents
Detection device and detection method Download PDFInfo
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- CN108572172A CN108572172A CN201710146599.3A CN201710146599A CN108572172A CN 108572172 A CN108572172 A CN 108572172A CN 201710146599 A CN201710146599 A CN 201710146599A CN 108572172 A CN108572172 A CN 108572172A
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- Prior art keywords
- test paper
- detection device
- secondary sample
- sample
- detection
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- 238000001514 detection method Methods 0.000 title claims abstract description 134
- 238000012360 testing method Methods 0.000 claims abstract description 150
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- 238000003908 quality control method Methods 0.000 claims description 10
- 238000007789 sealing Methods 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 5
- 238000003032 molecular docking Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 30
- 239000003795 chemical substances by application Substances 0.000 description 52
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 13
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- 230000002745 absorbent Effects 0.000 description 3
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- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
Embodiments of the present invention provide a kind of detection device and detection method.The detection device includes secondary sample-adding structure.Secondary sample-adding structure includes accommodating device and turned parts；By secondary sample-adding structure, various test paper can be directed to and carry out several functions extension, functional reagent can be housed using accommodating device, and with can the turned parts of precisely controlled unlatching carry out release function reagent；It can carry out release function reagent on the accurate position of test paper, in accurate flow nodes.To extend the detection function of test paper, and ensure the accuracy of testing result.
Description
Technical field
Embodiments of the present invention are related to detection technique field, more specifically, embodiments of the present invention are related to using examination
The detection device and detection method that paper is detected sample.
Background technology
Background that this section is intended to provide an explanation of the embodiments of the present invention set forth in the claims or context.Herein
Description recognizes it is the prior art not because not being included in this part.
Test paper detection device is generally used for the colouring information of Test paper detection line, can obtain target according to colouring information
The concentration level of substance.
Reactant (T line positions) is typically contained in test paper, when adding sample liquid to test paper, sample liquid can slowly climb
To T line positions and C line positions, the target substance in reactant and sample liquid, which carries out reaction, to develop the color in T line positions, can normally show
Existing detection line T, when sample liquid rises to C line positions, the nature controlling line C colour developings of C line positions.When nature controlling line C is effective, test paper detection
Device obtains the concentration level of target substance by detecting the colouring information of detection line T.
However, in some cases, since the developing concentration of detection line T is too low, it is difficult to which it is accurate to be obtained according to color signal
Detection data.For the bad sample of certain chromogenic reactions, possibly can not be detected by test paper detection device.
It, can be by adding this kind of functional reagent of sensitizer, to carry out enhanced sensitivity to testing result by the current understanding of inventor
The operations such as colour developing.But these functional reagents, due to lacking the carrier of steady implementation, so laboratory's item can only be directed to now
It uses under part, also cannot achieve for ordinary consumer or large-scale industrial application.
Invention content
It is various secondary to add when the test paper device detection of the prior art as it can be seen that for the reason of lack the carrier of steady implementation
The functional reagent added good can't be applied.
Therefore in the prior art, bad this of detection device chromogenic reaction is very bothersome process.
Thus, it is also very desirable to a kind of improved detection device, so that can be with steady implementation for the offer of various functions reagent
Carrier, with to detection structure carry out defuelling, rapidly to obtain accurate detection data.
In the present context, embodiments of the present invention are intended to provide a kind of detection device.
In the first aspect of embodiment of the present invention, a kind of detection device is provided, sample is examined using test paper
It surveys, the test paper has a sample application zone and detection colour developing area；The detection device have secondary sample-adding structure, it is described it is secondary plus
Spline structure includes accommodating device and turned parts；Accommodating cavity is formed in the accommodating device, the accommodating cavity is equipped with secondary
It is loaded agent；The accommodating device has the controllable openings towards the test paper；The controllable openings are directed at the two of the test paper
Secondary sample application zone；The controllable openings are opened in turned parts control, by the secondary sample-adding agent in the accommodating cavity discharge to
The test paper.
In some embodiments, the secondary sample application zone described in any of the above-described embodiment according to the present invention is located at the detection
The region developed the color between area and a sample application zone.
In some embodiments, the controllable openings described in any of the above-described embodiment according to the present invention include an outflow wall.
In some embodiments, the outflow wall described in any of the above-described embodiment according to the present invention is to form reeded wall
Body；Alternatively, the outflow wall is a membrane structure.
In some embodiments, the turned parts described in any of the above-described embodiment according to the present invention includes top, institute
Top is stated to correspond to towards the outflow wall；After the turned parts is driven, to flow out wall described in the top bursting, with
Secondary sample-adding agent in the accommodating cavity is discharged to the test paper.
In some embodiments, the top described in any of the above-described embodiment according to the present invention has front end centrum knot
Structure, the vertebral body structure are tapered to the other end towards the outflow wall from first end.
In some embodiments, top periphery described in any of the above-described embodiment according to the present invention formed there are one or
Multiple diversion trenches, the diversion trench extend to the direction of the test paper.
In some embodiments, the cross-sectional shape of the diversion trench described in any of the above-described embodiment according to the present invention is in V
Shape or U-shaped.
In some embodiments, the detection device described in any of the above-described embodiment according to the present invention further includes permeable,
The permeable carrier band has second two times sample-adding agent；Two after wall in the accommodating device are flowed out described in the top bursting
Secondary sample-adding agent discharges after being mixed with described second two times sample-adding agent to the test paper.
In some embodiments, the detection device described in any of the above-described embodiment according to the present invention further comprises propping up
Frame, accommodating device described in the rack bearing.
In some embodiments, the holder described in any of the above-described embodiment according to the present invention also has an aerial portion, institute
It states aerial portion and forms the overhead space passed through for the test paper, the overhead space is by the accommodating device and the test paper interval
Certain distance.
In some embodiments, the accommodating device described in any of the above-described embodiment according to the present invention can by bindiny mechanism
Assembly ground connects the holder.
In some embodiments, the detection device described in any of the above-described embodiment according to the present invention further includes permeable,
The holder have release channel, described permeable be located at the release channel top, the release channel openly towards
The test paper；After flowing out wall described in the top bursting, the top supports described permeable so that the permeable warp
The release channel comes close to or in contact with downwards the test paper, and the secondary sample-adding agent is through described permeable release to the test paper.
In some embodiments, the holder described in any of the above-described embodiment according to the present invention has plummer, for holding
Described permeable is carried, the plummer is located at the release channel top and forms step.
In some embodiments, the release channel described in any of the above-described embodiment according to the present invention corresponds to the top
Guide groove is offered, the top is guided with orientation.
In some embodiments, permeable described in any of the above-described embodiment according to the present invention be natural fiber filter paper,
Chemical fibre filter paper or glass fiber filter paper.
In some embodiments, the detection colour developing area described in any of the above-described embodiment according to the present invention includes Quality Control colour developing
Area and testing result colour developing area；Normal colour developing and/or testing result colour developing area's color conspicuousness parameter in Quality Control colour developing area
In the case of predetermined threshold, drive the turned parts movement to carry out secondary sample-adding.
In some embodiments, the accommodating cavity described in any of the above-described embodiment according to the present invention has an open end, institute
It states turned parts to be located in the accommodating cavity, the turned parts further comprises that main body, the main body are slidably mounted on
For the accommodating cavity to form a seal cavity, external drive structure drives the turned parts to move by the open end.
In some embodiments, the accommodating cavity described in any of the above-described embodiment according to the present invention is cylindrical cavity, described
The main body of turned parts is the shape being adapted to cylindrical cavity, and the main body of the turned parts is provided at least one layer of sealing ring.
In some embodiments, the accommodating device described in any of the above-described embodiment according to the present invention has a flexible shell
Body, by driving the turned parts to move flexible shell pressure.
In some embodiments, the detection device described in any of the above-described embodiment according to the present invention further includes a shell
Body, the shell offer adding mouth, observation window and secondary sample-adding operate interface；The adding mouth is aligned the primary of the test paper
Sample application zone；The observation window is aligned the detection colour developing area of the test paper；The secondary sample-adding structure is grasped by the secondary sample-adding
It is installed on the shell as interface.
In some embodiments, the secondary sample-adding operate interface described in any of the above-described embodiment according to the present invention is opened for one
Mouth is put, the accommodating device passes through the holder assembly connection in the opening port and opening port.
In some embodiments, the shell described in any of the above-described embodiment according to the present invention includes first shell and second
Shell, the first shell are combined with second shell docking, form cavity between for accommodating the test paper.
In some embodiments, the secondary sample-adding agent described in any of the above-described embodiment according to the present invention is sensitizer.
In the second aspect of embodiment of the present invention, a kind of detection method is provided, is included the following steps：
Prepare a test paper, the test paper has a sample application zone and detection colour developing area；
Preparing a carrier band has the container of secondary sample-adding agent, the container to have the controllable openings towards the test paper；
It is once loaded to a sample application zone；
The controllable openings are aligned to the secondary sample application zone of the test paper；
The controllable openings of the container are opened in control, the secondary sample-adding agent are discharged to the test paper, with to primary
Test paper after sample-adding carries out secondary sample-adding.
In some embodiments, any of the above-described embodiment according to the present invention, providing permeable carrier band has another kind secondary
It is loaded agent；It waits for after being once loaded detection, permeable is positioned on the test paper, and by the secondary sample-adding agent of the container
It is filled to described permeable so that the secondary sample-adding agent of the container is mixed with the secondary sample-adding agent of another kind in described permeable
After discharge to the test paper.
In some embodiments, any of the above-described embodiment according to the present invention, the secondary sample-adding agent is sensitizer, to one
The testing result of secondary sample-adding carries out enhanced sensitivity colour developing.
In some embodiments, any of the above-described embodiment according to the present invention, the controllable openings include an outflow wall,
The controllable openings that the container is opened in middle control include flowing out wall described in the top bursting using turned parts, by described two
Secondary sample-adding agent is discharged to the test paper.
In some embodiments, any of the above-described embodiment according to the present invention further comprises using in the top
The one or more diversion trenches of periphery setting, by the secondary sample-adding agent water conservancy diversion to the test paper.
In some embodiments, any of the above-described embodiment according to the present invention, further comprising will be described using sealing element
Turned parts is movably sealed in the drum ladle with open end.
In some embodiments, any of the above-described embodiment according to the present invention, further comprises by having flexible shell
The sealing container of body presses, and drives the turned parts to move, so as to flow out wall described in the top bursting.
In some embodiments, any of the above-described embodiment according to the present invention, further comprises：
Permeable, which is positioned on a step, makes described permeable certain distance is isolated with the test paper；
Described in the top bursting flow out wall after, the top supports described permeable so that described permeable to
The lower contact test paper, the secondary sample-adding agent is through described permeable release to the test paper.
In some embodiments, any of the above-described embodiment according to the present invention, wherein detection colour developing area includes Quality Control
Develop the color area and testing result colour developing area, and the method further includes in Quality Control colour developing area, normally colour developing and/or detection are tied
Fruit develops the color area's color conspicuousness parameter less than in the case of predetermined threshold, driving the turned parts to move to carry out secondary add
Sample.
According to the detection device and detection method of embodiment of the present invention, it will be understood by those skilled in the art that phase
For the prior art, it is provided with secondary sample-adding structure for test paper, various test paper can be directed to and carry out several functions extension, it can
House functional reagent using accommodating device, and with can the turned parts of controlled unlatching carry out release function reagent；It can try
Carry out release function reagent on the accurate position of paper, in accurate flow nodes.To extend the detection function of test paper, and ensure
The accuracy of testing result.
Description of the drawings
Detailed description below, above-mentioned and other mesh of exemplary embodiment of the invention are read by reference to attached drawing
, feature and advantage will become prone to understand.In the accompanying drawings, if showing the present invention's by way of example rather than limitation
Dry embodiment, wherein：
Fig. 1 schematically shows structure of the detecting device schematic diagram according to a first embodiment of the present invention；
Fig. 2 schematically shows the schematic cross-sectional views of the line A-A in Fig. 1；
Fig. 3 schematically shows the cross-sectional view of secondary sample-adding structure according to a first embodiment of the present invention；
Fig. 4 schematically shows the structural schematic diagrams of turned parts according to a first embodiment of the present invention；
Fig. 5 schematically shows detection device using process diagram one according to a first embodiment of the present invention；
Fig. 6 schematically shows detection device using process diagram two according to a first embodiment of the present invention；
Fig. 7 schematically shows structure of the detecting device schematic diagram according to a second embodiment of the present invention；
Fig. 8 schematically shows detection device schematic cross-sectional view according to a third embodiment of the present invention；
Fig. 9 schematically shows secondary sample-adding structural profile structural schematic diagram according to a third embodiment of the present invention.
In the accompanying drawings, identical or corresponding label indicates identical or corresponding part.
1, test paper；11, a sample application zone；12, detection colour developing area；121, Quality Control colour developing area；122, testing result colour developing area；
13, sample pad；14, absorbent filter；15, colloidal gold pad；100, secondary sample-adding structure；2, holder；21, plummer；22, release is logical
Road；23, guide groove；24, portion is maked somebody a mere figurehead；25, connecting interface；3, accommodating device；30, controllable openings；31, accommodating cavity；32, open
End；33, wall is flowed out；34, outer wall；35, hush panel；36, bindiny mechanism；4, turned parts；41, main body；411, fixing groove；412、
Sealing ring；42, top；421, vertebra rib；422, diversion trench；5, secondary sample-adding agent；6, permeable；70, chassis；7, shell；71、
Adding mouth；72, observation window；73, secondary sample-adding operate interface；74, first shell；75, second shell；76, installing zone；8, it presses
Bar.
3 ', accommodating device；31 ', accommodating cavity；32 ', flexible shell；33 ', wall is flowed out；34 ', internals.
Specific implementation mode
The principle and spirit of the invention are described below with reference to several illustrative embodiments.It should be appreciated that providing this
A little embodiments are used for the purpose of making those skilled in the art can better understand that realizing the present invention in turn, and be not with any
Mode limits the scope of the invention.On the contrary, these embodiments are provided so that the disclosure is more thorough and complete, and energy
It is enough that the scope of the present disclosure is completely communicated to those skilled in the art.
According to the embodiment of the present invention, it is proposed that a kind of detection device and detection method.
Herein, it is to be understood that involved term test paper, refer to chemicals it is impregnated, it can be passed through
A kind of paper existing for Cucumber in color change detecting liquid or gas.E.g. with the dipped dry paper of indicator or reagent
Item.For another example colloid gold test paper, general from one end to the other end includes mainly sample pad, colloidal gold pad, carrier and absorbent filter.And
This several part can also be each attached on a baseboard material.Above-mentioned carrier is appreciated that detection colour developing area.In addition, in attached drawing
Any number of elements is used to example and unrestricted and any name is only used for distinguishing, without any restrictions meaning.
Below with reference to several representative embodiments of the present invention, the principle and spirit of the invention are illustrated in detail.
Summary of the invention
The inventors discovered that in some cases, since the detection line concentration of test paper is too low, it is difficult to be obtained according to color signal
Obtain accurate detection data.For the bad sample of certain chromogenic reactions, possibly can not be detected by test paper detection device.
It is therefore desirable to be directed to test paper secondary sample-adding structure is arranged, various test paper can be directed to and carry out several functions expansion
Exhibition can house functional reagent using accommodating device, and with can the turned parts of controlled unlatching carry out release function reagent；It can
Carry out release function reagent on the accurate position of test paper, in accurate flow nodes.To extend the detection function of test paper, and
Ensure the accuracy of testing result.
After the basic principle for describing the present invention, lower mask body introduces the various non-limiting embodiment party of the present invention
Formula.
Illustrative methods
Embodiments of the present invention can be applied to applicable any scene.
An embodiment of the present invention provides a kind of detection methods, it is believed that mainly includes the following steps：
Prepare a test paper, test paper has a sample application zone and detection colour developing area；
Preparing a carrier band has the container of secondary sample-adding agent, container to have the controllable openings towards test paper；
It is once loaded to test paper；
It waiting for after being once loaded detection, the controllable openings of container are opened in control, and secondary sample-adding agent is discharged to test paper,
To carry out secondary sample-adding to being once loaded the test paper after detecting.Secondary sample-adding designated herein refers to being carried out using subject sample
After being loaded for the first time, sample or the action of other functional reagents are filled to test paper carrier again.Secondary sample-adding agent be appreciated that
Test paper filling former subject sample or various functions reagent, such as screening agent, color developing agent, sensitizer or diluent etc. be possible into
Row defuelling or the sample repeatedly filled.
In another concrete operations example, can also provide permeable carrier band has another secondary sample-adding agent；It waits for once being loaded inspection
After survey, permeable is positioned on test paper, and by the secondary sample-adding agent of container to permeable fill, with in permeable
It is discharged to test paper after another secondary sample-adding agent mixing.Permeable for can for liquid by material, may be selected to be natural fiber
Filter paper, chemical fibre filter paper or glass fiber filter paper, are not limited thereto system.In one specific practical example, permeable is selected as glass
Glass fiber filter paper.In use, permeable can will be in secondary sample-adding agent equably water conservancy diversion to test paper.
Secondary sample-adding agent can be sensitizer, in order to carry out enhanced sensitivity colour developing to the testing result being once loaded.According to this hair
Bright concrete application example, wherein sensitizer can also include the two or more components stored respectively.Specific carrier band mode is upper
By the agency of is stated in configuration example, details are not described herein.The efficient sensitizer mixed in this use can further improve enhanced sensitivity effect
Rate.If there is demand to use three components or more component sensitizer, it may be considered that place more permeable, each permeable can carry not
Same component.With reference to above description, it will be understood by those skilled in the art that compared with the existing technology, with above-described embodiment
Detection method is operated for test paper provided with secondary sample-adding, can be directed to various test paper and be carried out several functions extension, available
Container houses functional reagent, and with can precisely controlled unlatching container carry out release function reagent；It can be in the accurate of test paper
On position, carry out release function reagent in accurate flow nodes.To extend the detection function of test paper, and ensure testing result
Accuracy.
Example devices
After describing the method for exemplary embodiment of the invention, next, showing the present invention referring to figs. 1 to Fig. 9
The detection device introduction of example property embodiment is described as follows：
First embodiment
Fig. 1 schematically shows structure of the detecting device schematic diagram according to a first embodiment of the present invention；Fig. 2 is schematically
Show the schematic cross-sectional view of the part-structure of the line A-A in Fig. 1；Fig. 3 is schematically shown according to the present invention again
The cross-sectional view of secondary sample-adding structure in first embodiment.
As shown in Figure 1 to Figure 3, first embodiment of the invention provides a kind of detection device, can be directed to using various test paper
Corresponding subject sample is detected.It should be understood that test paper 1 generally has a sample application zone 11 and detection colour developing area 12.
Sample application zone 11 can be understood as the region for reagent such as sample to be added, and be normally at one end of test strips, can such as have
The sample pad 13 being connect with detection colour developing area 12；It is understood that sample pad 13 also can be integrated into one with detection colour developing area 12
Body.Detection colour developing area 12 is it is to be appreciated that the carrier of detection reagent detects colour developing 12 material of area for example for carrying detection reagent
It is filter paper, nitrocellulose membrane, miillpore filter.Detection colour developing area 12 generally comprises Quality Control colour developing area 121 and testing result colour developing area
122.Detection colour developing 12 other end of area can connect an absorbent filter 14.Above-mentioned test paper 1 may be selected to utilize a chassis 70 into luggage
With fixation, chassis 70 can accordingly be connected with choice arrangement and positioning element.
In this embodiment, also there is detection device secondary sample-adding structure 100 (see Fig. 3), secondary sample-adding structure 100 can lead
To include accommodating device 3 and turned parts 4.In order to carry out secondary sample-adding to test paper 1 using secondary sample-adding structure 100, with reality
Secondary sample-adding is now carried out on demand.Secondary sample-adding designated herein refers to after subject sample be loaded for the first time, again to test paper
Carrier fills sample or the action of other functional reagents.The reagent of secondary sample-adding, which is appreciated that test paper, fills former subject sample
Or various functions reagent, such as screening agent, color developing agent, sensitizer or diluent etc. are possible to carry out defuelling or repeatedly add
The sample of note.
In one embodiment, primary sample-adding is used for target sample to be tested, secondary sample-adding to be added for sensitizer to be added
In the color conspicuousness for increasing detection line.
In one embodiment, one holder 2 of setting also may be selected in secondary sample-adding structure 100, to be used for filling with accommodating device 3
Match.Secondary sample-adding structure 100 may be selected to be fixed on chassis 70, may be selected detachably to be assemblied in the connector of 2 bottom of holder
On chassis 70, connector may be selected to be assembled with chassis 70 in a manner of being spirally connected, be clamped, rivet, weld etc., with snap fit in figure
Carry out example.
In one embodiment, the selection of holder 2 is set between detection colour developing area 12 and a sample application zone 11, that is, is located at along examination
On region between the detection colour developing area 12 and a sample application zone 11 of test paper 1 on 1 length direction of paper.So as to detect
The position developed the color between area 12 and a sample application zone 11 carries out secondary sample-adding.It should be understood that in detection colour developing area 12 and one
Secondary sample-adding is carried out between secondary sample application zone 11, the reagent of secondary sample-adding is identical as sample chromatography direction, first, inspection can be normally entered
Survey colour developing area 12；Second is that chromatography process can be sufficiently mixed with a loaded sample；Third, a sample application zone 11 with it is secondary
Sample-adding structure 100 will not interfere with each other；Fourth, primary sample-adding and secondary sample-adding can be distinguished effectively, maloperation is avoided.
It will be understood by those skilled in the art that secondary sample-adding structure carries out test paper the position of secondary sample-adding, except upper
It gives an account of between the detection colour developing area 12 to continue and a sample application zone 11, can also there is other selections, such as detection colour developing area or through it
Connection member is again to target area between it.By taking colloid gold test paper as an example, in sample application zone of colloid gold test paper and detection colour developing area one
As can also set colloidal gold pad, secondary sample-adding structure can select alignment colloidal gold pad to carry out secondary sample-adding.
Holder 2 is for carrying and being fixedly mounted accommodating device 3, it is also possible that being separated between accommodating device 3 and test paper 1
Certain distance.It will be understood by those skilled in the art that holder 2 therein is only intended to fixed accommodating device 3, structure shape
Formula does not limit.Utilize the installation position of holder 2, it may be determined that be directed to the exact position of the secondary sample-adding of test paper.And profit
With the interval action of holder 2, it is ensured that in the case that without secondary sample-adding, do not interfere with the detection being once loaded or chromatography
Process, to avoid impacting to being once loaded detection.In one embodiment, secondary sample-adding structure 100 may not include independence
Supporting structure, secondary sample-adding structure for example can carry and install accommodating fill by housing the integrally formed shell of test paper
It sets.
In this embodiment, an accommodating cavity 31 has been could be alternatively formed in accommodating device 3, in order to accommodating cavity 31 accommodating one
The secondary sample-adding agent 5 (see Fig. 3) of kind.Accommodating device 3 can be provided with the controllable openings 30 towards test paper 1.Wherein accommodating device 3
It is mainly used for housing secondary sample-adding agent 5, so accommodating device 3 can be the variously-shaped or works for meeting container function, example
Such as cylindrical shape, square tube shape, spheroidal, elliposoidal or capsule shape container shapes.It should be understood that controllable openings 30 can be
The structure of the various controlled unlatchings of energy, such as removable cover structure, dynamic seal structure, fragile membrane configuration or fragile wall surface knot
Structure.It will be understood by those skilled in the art that it is various can it is controlled open a controllable openings structure be applicable as opening
Component 4, here without specific limitation.Citing is such as：The direct acting or tilting member with spine driven by resetting spring；Certainly it resets
Spring also can be replaced other accumulators (such as piston type or gas type accumulator).
As in the specific example enumerated in figure, accommodating device 3 can be a kind of cylindrical vessel, wherein bottom is controllable
Opening 30 may include an outflow wall 33, and 3 outside of accommodating device is circular ring shape outer wall 34, the open end 32 on top may be selected assembly or
Connect a hush panel 35.And it is understood that outflow wall 33 be that can be opened by steering test paper direction, it is secondary in order to carry out
Sample-adding.Outflow wall 33 may be selected to be to form reeded wall body；Alternatively, outflow wall 33 can be a membrane structure, in order to open
Secondary sample-adding agent 5 can be discharged after opening downwards.Secondary sample-adding agent 5 to test paper it is to be appreciated that fill former subject sample or various work(
Energy reagent, such as screening agent, color developing agent, sensitizer or diluent etc. are possible to the sample for carrying out defuelling or repeatedly filling
Product.
Turned parts 4 may include main body 41 and top 42, and top 42 is corresponded to towards outflow wall 33.In order to open
After component 4 is driven, wall 33 is flowed out with 42 bursting of top, by secondary sample-adding agent 5 release in accommodating cavity 31 to test paper 1.
Turned parts 4 may be alternatively located at outside accommodating cavity 31, or selection is located in accommodating cavity 31.
In one concrete application example, after primary sample-adding, if Quality Control colour developing area 121 normally develops the color under situation, then unlatching is driven
Component 4 is moved to carry out secondary sample-adding.In this way, ensureing that sample to be tested carries out that when being once loaded permeable 6 will not be stained with, can avoid
Secondary sample-adding action influences the Quality Control colour developing of test paper, ensures to carry out next step operation again after examining the test paper whether normal.
With reference to above description, it will be understood by those skilled in the art that compared with the existing technology, with above-described embodiment
Detection device is provided with secondary sample-adding structure for test paper, can be directed to various test paper and carry out several functions extension, utilize branch
Frame realizes the secondary sample-adding structure of assembly connection, can house functional reagent using accommodating device, and precisely controlled can open
The turned parts opened carrys out release function reagent；Work(can be discharged on the accurate position of test paper, in accurate flow nodes
It can property reagent.To extend the detection function of test paper, and ensure the accuracy of testing result.
The concrete structure example of each component in secondary sample-adding structure 100, as shown in Figure 1 to Figure 3, branch are introduced in conjunction with attached drawing as follows
Frame 2 may be selected to be a plastic part, such as polypropylene (PP), may be selected, with injection molding, to be not limited thereto system.Holder 2 can be
Upper table downwards concave shape at plummer 21 and release channel 22.The size of plummer 21 can be more than 22 (radial ruler of release channel
It is very little), in order to form a step between, with this step, movably support places permeable 6.The step can make permeable
Piece and the test paper of lower section are spaced apart, ensure that primary sample-adding chromatography and process color are not influenced by secondary sample-adding structure.
Plummer 21 is connected to release channel 22, forms the channel of a holder 2 up and down, and this channel can be openly towards examination
Paper 1.After holder 2 and accommodating device 3 assemble, plummer 21 is aligned the outflow wall 33 of accommodating device 3 with release channel 22 simultaneously
And the top 42 of turned parts 4.
According to above-mentioned concrete structure example, the one or both sides of release channel 22 can also be equipped with guide groove 23, and guide groove 23 is
It is set to the concave and is formed by 22 outer wall of release channel, 23 size of guide groove corresponds to the outside dimension of top 42, in order to top 42
Into after release channel 22, the movement track of top 42 can be limited using guide groove 23, occurred in avoiding top 42 from advancing
Offset.
According to above-mentioned concrete structure example, after wall 33 is flowed out in 42 bursting driven of top, top 42 can be supported permeable
Piece 6 so that permeable 6 contacts downwards test paper 1 through release channel 22, in this way, the secondary sample-adding agent 5 in accommodating device 3 can be through saturating
Water piece 6 is discharged to test paper 1.
With reference to shown in Fig. 2 and Fig. 3, in the concrete structure example of holder 2, holder 2 can also have an aerial portion 24, make somebody a mere figurehead portion 24
The overhead space passed through for test paper 1 is formed, overhead space can be spaced apart by accommodating device 3 and test paper 1.It can ensure
In the case of secondary sample-adding, the detection being once loaded or chromatography process are not interfered with, to avoid to being once loaded detection
It impacts.
As shown in Figure 1 to Figure 3, holder 2 can also have the connecting interface 25 towards accommodating device 3, so as to accommodating device 3
36 assembly connection of bindiny mechanism of lower part, it will be understood by those skilled in the art that connection type between the two may be selected
The modes such as to be spirally connected, being clamped, weld, example is carried out with snap fit in figure.As shown, connecting interface 25 is from holder 2
Two card holes that top surface opens up downwards, corresponding, the bindiny mechanism 36 of 3 lower part of accommodating device is two card columns, bindiny mechanism 36
Card column the location structures such as convex block can also be set.
According to another concrete structure example, accommodating device 3 is whole can be cylindrical, and turned parts 4 is located in accommodating cavity 31, opens
It is the shape being adapted to cylindrical cavity to open 4 main body 41 of component, and 41 periphery of main body is offered an at least fixing groove 411, fixed with this
There is at least one layer of sealing ring 412, turned parts 4 is movably sealed in accommodating cavity 31 whereby.The main body 41 of turned parts 4 is removable
It is sealed in open end 32 dynamicly, external drive structure drives turned parts 4 to carry out piston motion by open end 32.With this structure
It can prevent the secondary sample-adding agent 5 in accommodating device 3 from leaking out.Using siphon principle, two can also be controlled according to down stroke
The injection rate of secondary sample-adding.Meanwhile using movably sealing structure, it can also push secondary sample-adding agent 5 to be flowed to test paper direction, keep away
Exempt from because work angle problem influences the filling of secondary sample-adding agent 5.
According to concrete structure example as shown in Figure 4, top 42 is at least vertebral body structure in front end, and vertebral body structure is opened by close
The one end for opening 4 main body 41 of component is tapered to the other end, and vertebral body structure can be located at 42 front end of top, can also be by main body 41 1
It is straight to extend to 42 top of top.Spine structure is formed with vertebral body structure, wall 33 is flowed out convenient for puncturing with this.42 periphery of top
There can be multiple vertebra ribs 421 extended downwardly, 42 periphery of top may also be formed with one or more diversion trenches 422, diversion trench
422 are respectively positioned between two neighboring vertebra rib 421.Diversion trench 422 can be extended from 4 main body 41 of turned parts to the direction of test paper 1.It leads
The cross-sectional shape of chute 422 can V-shaped or diversion trench 422 cross-sectional shape can take the shape of the letter U.Outflow wall 33 can punctured
Afterwards, secondary sample-adding agent 5 is flowed out using 422 water conservancy diversion of diversion trench, avoids blocking.
According to another concrete application example, permeable 6 can carry second two times sample-adding agent 5, second two times sample-adding agent 5
It can select to be solid states, such as freeze-dried powder.It is secondary in accommodating device 3 after permeable 6 contact test paper 1 is supported in top 42
Sample-adding agent 5 discharges after being mixed with second two times sample-adding agent 5 in permeable to test paper 1.Features examination can be met in this way
The demand of agent, is only just mixed when in use, is gone bad after can avoid the long-term mixing of the two.
It may be selected to be natural fiber filter paper, chemical fibre filter paper or glass fiber filter paper for permeable 6, be not limited thereto
System.In one specific practical example, permeable 6 is selected as glass fiber filter paper.In use, permeable 6 can be equal by secondary sample-adding agent 5
On even ground water conservancy diversion to test paper.
According to a concrete application example of the invention, secondary sample-adding agent 5 may be selected to be a kind of sensitizer, in order to be surveyed in a sample-adding
After examination, the sensitizer is filled to test paper 1 using secondary sample-adding structure 100, when sensitizer enters the detection zone of test paper, may make
Detection line color depth is deepened.With this so that testing result is accurately read.Furthermore it is also possible to greatly shorten detection time.
Average 12 minutes detection times of traditional detection mode can be foreshortened to average using the scheme of the application example through actual test
6 minutes or so.
According to a concrete application example of the invention, wherein sensitizer can also include the two or more components stored respectively.
Specific carrier band mode by the agency of in above structure example, details are not described herein.The efficient sensitizer mixed in this use, can
Further increase enhanced sensitivity efficiency.If there is demand to use three components or more component sensitizer, it may be considered that place permeable more
6, each permeable 6 can carry different components.
Fig. 5 schematically shows detection device using process diagram one according to a first embodiment of the present invention；Fig. 6 shows
Show detection device using process diagram two according to a first embodiment of the present invention to meaning property.As shown in the figure.It can utilize
Motor-driven feed head drives turned parts 4, specifically, after can first removing hush panel 35, is placed on accommodating device 3
One compression bar 8 recycles the downward moulded rod 8 of feed head, flows out wall 33 with 42 bursting of top, by secondary in accommodating cavity 31 plus
Sample agent 5 is discharged to test paper 1.
According to an embodiment, for detection device when being detected using colloid gold test paper, secondary sample-adding structure 100 is corresponding
The colloidal gold pad position of corresponding colloid gold test paper may be selected in the position of secondary sample-adding.
Second embodiment
According to Fig.7, the embodiment of the present invention provides a kind of detection device, relative to above-mentioned first embodiment, detection dress
It further includes a shell 7 to set, and shell 7 one operates can offer adding mouth 71, observation window 72 and secondary sample-adding operate interface on surface
73.Adding mouth 71 can be aligned a sample application zone 11 of test paper 1.Observation window 72 can be aligned the detection colour developing area 12 of test paper 1.It is secondary to add
For sample operate interface 73 between adding mouth 71 and observation window 72, secondary sample-adding operate interface 73 can be an opening port, house dress
3 are set by 2 assembly connection of holder in opening port and opening port.Secondary sample-adding structure 100 passes through secondary sample-adding operate interface 73
It is installed on shell 7.Wherein, 2 main body of holder is mountable in shell 7, and 2 top of holder can protrude from secondary sample-adding operate interface 73
Opening port, in order to which accommodating device 3 can be by connection structure assembly connection in holder 2.In this way, detection device is being packed and is being transported
Two parts can be divided into defeated, a part is the component being fixedly mounted in shell 7, and another part is accommodating device 3, user
Above-mentioned two parts are assembled again before, Package size can be obviously reduced, save transportation cost.
Shell 7 can be plastics or other materials, may be selected to use injection molding.According to actual production conditions, shell 7 can
To include first shell 74 and second shell 75, first shell 74 is combined with the docking of second shell 75, combination may be selected be
In a manner of being spirally connected, be clamped, rivet, weld.Installing zone 76 can be formed between first shell 74 and second shell 75.Test paper 1
It is mountable in installing zone 76 with holder 2, the plummer 21 of holder 2 is aligned secondary sample-adding operate interface 73.
In one embodiment, holder 2 can be considered a part for secondary sample-adding structure.In another embodiment, holder 2
Also it can be considered a part for shell 7, can be integrally formed or be removed and assembled into type with shell.
Test paper detection device may also include imaging sensor, and inspection is obtained for absorbing the colouring information of detection line on test paper
Survey numerical value.It may include such as Quick Response Code identification pattern on shell 7, the imaging sensor in detection device can be by absorbing and identifying
The mark determines the type of detection device with this, so as to based thereon determine whether to be loaded, the time of sample-adding, after sample-adding between
How often the presupposed informations such as the camera shooting of line are detected.
3rd embodiment
According to Fig.8, the embodiment of the present invention provides a kind of detection device, real relative to above-mentioned first embodiment and second
Example is applied, the main distinction is, accommodating device 3 ' includes mainly a flexible shell 32 ', an internals 34 ' and an outflow wall 33 ', flexibility
Shell 32 ' surrounds accommodating cavity 31 ' jointly with internals 34 '.Internals 34 ' are columnar structured, and inside forms cylindrical space, internals
34 ' peripheries can form convex-concave structure, and internals 34 ' can be hard plastic structure, such as polypropylene (PP).
Flexible shell 32 ' can be flexible material, such as thermoplastic polyurethane elastomer rubber (TPU)；Flexible shell 32 ' is whole
External shape is in hat shape, and 32 ' opening portion of flexible shell is incorporated in 34 ' periphery of internals, and combination can be that splicing or hot melt connect
It connects, bond strength is increased by convex-concave structure.Flexible shell 32 ' is internal also to form cylindrical space, the internal cylinder with internals 34 '
Shape space articulation forms accommodating cavity 31 '.An indent can also be arranged in 32 ' top of flexible shell, and corresponding inside can also be
One protrusion can so be convenient for external drive to press.
Outflow wall 33 ' may be incorporated in the opening of flexible shell 32 ' and internals 34 ', and in the present embodiment, flowing out wall 33 ' is
One sealing film, material can be composite material or metal material.
Turned parts 4 equally may be selected to be set in accommodating cavity 31 '.The structure of turned parts 4 is referred to aforementioned implementation
Example.
It may be selected to support accommodating device 3 ', holder 2 that connection structure can also be arranged with accommodating device 3 ' with holder 2 in shell 7,
It may be selected to be attached in a manner of being spirally connected, be clamped, rivet, weld etc..Also it may be selected to utilize first shell 74 and second shell
75 and holder 23 ' fixed clamp of accommodating device is fixed jointly.
Accommodating device 3 ' in the present embodiment, sealing effect is more preferable, and the secondary sample-adding agent 5 in accommodating cavity 31 ' can not possibly be to
Outer spilling.If with first shell 74 and second shell 75 and holder 2 jointly by the fixed side of 3 ' fixed clamp of accommodating device
Formula, and combine with transparent material selectively closing adding mouth 71 and observation window 72.Not only waterproof can be achieved in accommodating device 3 ', whole
Detection device can also realize waterproof.
As shown in Figure 9, according to an embodiment, detection device using colloid gold test paper when being detected, secondary sample-adding
The position of the colloidal gold pad 15 of corresponding colloid gold test paper may be selected in the position of the corresponding secondary sample-adding of structure 100.
It should be noted that although being referred to several types of devices or sub-device of detection device in above-detailed,
It is that this division is only not enforceable.In fact, according to the embodiment of the present invention, two or more above-described dresses
The feature and function set can embody in one apparatus.Conversely, the feature and function of an above-described device can be with
It is further divided into and is embodied by multiple devices.
In addition, although the operation of the method for the present invention is described with particular order in the accompanying drawings, this do not require that or
Hint must execute these operations according to the particular order, or have to carry out shown in whole operation could realize it is desired
As a result.Additionally or alternatively, it is convenient to omit multiple steps are merged into a step and executed by certain steps, and/or by one
Step is decomposed into execution of multiple steps.
Although by reference to several spirit and principle that detailed description of the preferred embodimentsthe present invention has been described, it should be appreciated that, this
It is not limited to the specific embodiments disclosed for invention, does not also mean that the feature in these aspects cannot to the division of various aspects
Combination is this to divide the convenience merely to statement to be benefited.The present invention is directed to cover appended claims spirit and
Included various modifications and equivalent arrangements in range.
Claims (22)
1. a kind of detection device is detected sample using test paper, the test paper has a sample application zone and detection colour developing area；
The detection device has secondary sample-adding structure, and the secondary sample-adding structure includes accommodating device and turned parts；
Accommodating cavity is formed in the accommodating device, the accommodating cavity is equipped with secondary sample-adding agent；The accommodating device has one
Controllable openings towards the test paper；
The controllable openings are directed at the secondary sample application zone of the test paper；
The controllable openings are opened in the turned parts control, and the secondary sample-adding agent in the accommodating cavity is discharged to the examination
Paper.
2. detection device as described in claim 1, wherein the secondary sample application zone is located at detection colour developing area and described one
Region between secondary sample application zone.
3. detection device as described in claim 1, wherein the controllable openings include an outflow wall.
4. detection device as claimed in claim 3, wherein the outflow wall is to form reeded wall body；Alternatively, the stream
It is a membrane structure to go out wall.
5. detection device as claimed in claim 3, wherein the turned parts includes top, the top corresponding surface
To the outflow wall；It, will be in the accommodating cavity to flow out wall described in the top bursting after the turned parts is driven
Secondary sample-adding agent discharge to the test paper.
6. detection device as claimed in claim 5, wherein the top has front end vertebral body structure, the vertebral body structure
It is tapered to the other end towards the outflow wall from first end.
7. detection device as claimed in claim 6, wherein the top periphery is formed with one or more diversion trenches, institute
Diversion trench is stated to the direction of the test paper to extend.
8. detection device as claimed in claim 7, wherein the cross-sectional shape of the diversion trench is V-shaped or U-shaped.
9. detection device as claimed in claim 5, wherein the detection device further includes permeable, the permeable carrier band
There are second two times sample-adding agent；Flowed out described in the top bursting secondary sample-adding agent after wall in the accommodating device with it is described
It is discharged to the test paper after second two times sample-adding agent mixing.
10. detection device as claimed in claim 5, wherein the detection device further comprises holder, the rack bearing
The accommodating device.
11. detection device as claimed in claim 10, wherein the holder also has an aerial portion, and the aerial portion is formed
For the overhead space that the test paper passes through, the overhead space is spaced apart by the accommodating device and the test paper.
12. detection device as claimed in claim 10, wherein the detection device further includes permeable, and the holder has
Release channel, the described permeable top for being located at the release channel, the release channel is openly towards the test paper；It is described
After flowing out wall described in the bursting of top, the top supports described permeable so that described permeable through the release channel
The test paper is come close to or in contact with downwards, and the secondary sample-adding agent is through described permeable release to the test paper.
13. detection device as claimed in claim 12, wherein the holder has plummer, for carrying described permeable,
The plummer is located at the release channel top and forms step.
14. detection device as described in claim 1, wherein detection colour developing area includes Quality Control colour developing area and testing result
Develop the color area；In Quality Control colour developing area, normally colour developing and/or testing result colour developing area's color conspicuousness parameter are less than predetermined threshold
In the case of, drive the turned parts movement to carry out secondary sample-adding.
15. such as claim 1 to 14 any one of them detection device, wherein the accommodating cavity has an open end, described to open
It opens component to be located in the accommodating cavity, the turned parts further comprises that main body, the main body are slidably mounted on described
For accommodating cavity to form a seal cavity, external drive structure drives the turned parts to move by the open end.
16. detection device as claimed in claim 15, wherein the accommodating cavity is cylindrical cavity, the master of the turned parts
Body is the shape being adapted to cylindrical cavity, and the main body of the turned parts is provided at least one layer of sealing ring.
17. such as claim 1 to 14 any one of them detection device, wherein the accommodating device has a flexible shell, leads to
It crosses and the turned parts movement is driven to flexible shell pressure.
Further include a shell 18. such as claim 1 to 14 any one of them detection device, the shell offer adding mouth,
Observation window and secondary sample-adding operate interface；The adding mouth is aligned a sample application zone of the test paper；The observation window is aligned institute
State the detection colour developing area of test paper；The secondary sample-adding structure is installed on the shell by the secondary sample-adding operate interface.
19. detection device as claimed in claim 18, wherein the secondary sample-adding operate interface is an opening port, the appearance
It sets device and passes through the holder assembly connection in the opening port and opening port.
20. detection device as claimed in claim 18, wherein the shell includes first shell and second shell, and described
One shell is combined with second shell docking, forms cavity between for accommodating the test paper.
21. such as claim 1 to 14 any one of them detection device, wherein the secondary sample-adding agent is sensitizer.
22. a kind of detection method, includes the following steps：
Prepare a test paper, the test paper has a sample application zone and detection colour developing area；
Preparing a carrier band has the container of secondary sample-adding agent, the container to have the controllable openings towards the test paper；
It is once loaded to a sample application zone；
The controllable openings are aligned to the secondary sample application zone of the test paper；
The controllable openings of the container are opened in control, the secondary sample-adding agent are discharged to the test paper, with to being once loaded
Test paper afterwards carries out secondary sample-adding.
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| CN201710146599.3A CN108572172A (en) | 2017-03-13 | 2017-03-13 | Detection device and detection method |
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| CN201710146599.3A CN108572172A (en) | 2017-03-13 | 2017-03-13 | Detection device and detection method |
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