CN108562662A - A method of Radix Curcumae Contained Serum is measured using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums - Google Patents
A method of Radix Curcumae Contained Serum is measured using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums Download PDFInfo
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Abstract
This application involves a kind of methods for analyzing Radix Curcumae decocting liquid Contained Serum using Thermo UPLC ITQ Orbitrap high resolution mass spectrums.The application uses the chemical composition in Thermo UPLC ITQ Orbitrap high resolution mass spectrums analysis hand Radix Curcumae Contained Serum to lay the first stone for next step research pharmacokinetics.
Description
Technical field：Radix Curcumae extract is measured using UPLC-LTQ-Orbitrap high resolution mass spectrums this application involves a kind of
The method of Contained Serum belongs to Analysis of Chinese Traditional Medicine and measures field.
Background technology：
Radix Curcumae Source of Drug Plants is more, and also there are different variations in the medicinal part different periods of history.《Tang materia medica》It says
" for this medicine seedling like turmeric, grey matter is red, and the last autumn goes out the stem heart, no reality.Root is helvolus.The sub- root in four sides is taken, is removed the peel, fire does it.Raw Sichuan and
Western army ... south of the Five Ridges person has reality, unbearably light like red bean model.”《Sixteen grades of doctoral candidate's academic dissertations of department of traditional Chinese medicine institute of this China
Grass covers label》Carry " the red simultaneous Huang of color, raw Sichuan person victory.Body circle has section, class cicada tripe person true.”《Compendium of Materia Medica》Also cloud " its seedling like ginger,
Root size such as finger, elder cun are permitted, and body, which is justified, band such as cicada abdomen shape, red in outer Huang." from the description deducibility of above-mentioned morphological feature
The source of Radix Curcumae is the axillary root stem of plant turmeric, rather than the root tuber of the present before the Ming Dynasty.About late Ming ＆ early Qing when, Radix Curcumae it is medicinal
Position is changed from rhizome to root tuber, such as the Qing Dynasty《The Illustrated Book on Plants》" Radix Curcumae, life Sichuan person are RADIX CURCUMAE from Sichuan of China to cloud, such as with root
Locust dung beetle tripe person is true.It is turmeric to contaminate yellow person ".《Chinese Pharmacopoeia》One is recorded when version in 2005, and Radix Curcumae is zingiberaceous plant
RADIX CURCUMAE, turmeric, Guangxi obtain the dried root of art or fluffy legendary ruler of great antiquity, the first of the Three August Ones's art.The above two practise respectively title " RADIX CURCUMAE " and " Radix Curcuma " remaining
It is practised by character difference and claims " Du Yujin " or " green silk Radix Curcumae ".Excavated after winter cauline leaf is withered, remove silt and radicula, steam or boil to
The saturating heart, it is dry.Property it is pungent, bitter, tremble with fear.Return liver, the heart, lung channel.Work(with promoting the circulation of qi addiction, heart fire-clearing upset-relieving, normalizing gallbladder to cure jaundice.It is mainly used for
Treatment Amenorrhea, dysmenorrhoea, chest and abdomen swelling and pain, shouting pain, pyreticosis coma, insane carbuncle go mad, yellow subcutaneous ulcer, urinate the diseases such as red.
Invention content：
We have obtained during the pharmacological property of early-stage study Radix Curcumae：Radix Curcumae water extract shows as cold in nature, Radix Curcumae alcohol extracting
Object shows as warm-natured；The cold temperature difference of Radix Curcumae extract is different to have certain positive correlation with its turmeric cellulose content.In order to further grind
Study carefully the cold material base of Radix Curcumae, it is objective, accurately, needs to analyze the ingredient in Radix Curcumae water extract Contained Serum, survey
It is fixed.During the studies above, a kind of method of analysis Radix Curcumae Contained Serum has been obtained.Specially：
It is special this application involves a kind of method for analyzing Radix Curcumae extract Contained Serum using UPLC-LTQ-Orbitrap
Sign is to take sample to be tested (Radix Curcumae Contained Serum), carries out pre-treatment, analyzed using UPLC-LTQ-Orbitrap, is identified
The group gone out in Contained Serum is divided into Curcumin (curcumin), Dihydrocurcumin (double hydrogen curcumins), 5-Hydroxy-1,
7-bis (4-hydroxyphenyl) -3-heptanone (bis- (4- the hydroxy phenyls) -3- heptanone of 5- hydroxyls -1,7-).
The application is using the Radix Curcumae extract in the method for UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums
Contained Serum sample is：Give Radix Curcumae extract gavage to rat, successive administration 16 days is anaesthetized, abdominal aortic blood, centrifugation,
Collect serum.Wherein Radix Curcumae extract preparation method is：Radix Curcumae is taken, is crushed, 40 mesh sieve is crossed, adds 10 times of amount water, first impregnates 0.5h,
Decoct 2 times, each 45min, filtration, merge decocting liquid, be concentrated into 40ml to get.
In method of the application using UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums, sample to be tested
Pre-treating method is：200 μ L of Radix Curcumae Contained Serum are taken, 1300 μ L of chromatography methanol are added, it is heavy to carry out albumen for fully shaking 20 seconds
It forms sediment, centrifuges (12000r/min, 10min), nitrogen drying adds 120 μ L of methanol, centrifuges (12000r/min, 15 seconds), take supernatant
To obtain the final product.
In method of the application using UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums, chromatographic condition is：
Chromatographic condition：Chromatographic column：Thermo Scientific Syncronic (C18,100mm × 2.1mm, 1.7 μm), mobile phase：A
To contain the methanol of 0.1% formic acid (percent by volume), B is the water containing 0.1% glacial acetic acid (percent by volume), gradient elution
Program：0-20min, 10%A, 90%B；15-20min, 100%A, 0%B；20-25min, 30%A, 70%B.Flow velocity
0.30mL/min, column temperature：55 DEG C, sample size：1.5 μ L, Detection wavelength：265nm；
In method of the application using UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums, Mass Spectrometry Conditions：Matter
Spectrum analysis is using the quadrupole rod orbit ion trap mass spectrograph for being equipped with thermoelectricity esi ion source.Negative ions source voltage is respectively
3.7ky and 3.5kv；Capillary heating temperature is：320℃；Sheath atmospheric pressure：30psi；Assist gas pressure power：10psi.Volume heats
300 DEG C of evaporating temperature.Sheath rises and auxiliary gas is nitrogen.Collision gas is nitrogen, pressure 1.5mTorr.Level-one sweep parameter
For：Resolution ratio 7000, automatic growth control target are 1 × 106, maximum isolation time 50ms, mass-to-charge ratio scanning range 150-
1500.Using data dependence scan pattern, design parameter is for compound component identification：Resolution ratio 17500, automatic growth control mesh
It is designated as 1 × 105, maximum isolation time 50ms, maximum 10 ion scan secondary fragments, dynamic exclusion.
The application using UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serum method in, liquid matter system by
2.2 SP1.48 softwares of Xcalibur control, and data acquisition and measured portions processing are operated by the software.
A method of Radix Curcumae Contained Serum is analyzed using UPLC-LTQ-Orbitrap high resolution mass spectrums, which is characterized in that
200 μ L of Radix Curcumae extract Contained Serum are taken, 1300 μ L of chromatography methanol are added, fully shaking 20 seconds carries out albumen precipitation, centrifugation
(12000r/min, 10min), nitrogen drying, adds 120 μ L of methanol, centrifuges (12000r/min, 15 seconds), takes supernatant sample introduction.Its
In, chromatographic condition is：Chromatographic condition：Chromatographic column：Thermo Scientific Syncronic (C18,100mm × 2.1mm,
1.7 μm), mobile phase：A is the methanol containing 0.1% formic acid (percent by volume), and B is to contain 0.1% glacial acetic acid (volume basis
Than) water, gradient elution program：0-20min, 10%A, 90%B；15-20min, 100%A, 0%B；20-25min, 30%A,
70%B.Flow velocity 0.30mL/min, column temperature：55 DEG C, sample size：1.5 μ L, Detection wavelength：265nm；Mass Spectrometry Conditions：Mass spectral analysis
Using the quadrupole rod orbit ion trap mass spectrograph for being equipped with thermoelectricity esi ion source.Negative ions source voltage be respectively 3.7kv and
3.5ky；Capillary heating temperature is：320℃；Sheath atmospheric pressure：30psi；Assist gas pressure power：10psi.Volume heating evaporation temperature
300℃.Sheath gas and auxiliary gas are nitrogen.Collision gas is nitrogen, pressure 1.5mTorr.Level-one sweep parameter is：Resolution ratio
7000, automatic growth control target is 1 × 106, maximum isolation time 50ms, mass-to-charge ratio scanning range 150-1500.Compound
Using data dependence scan pattern, design parameter is for component identification：Resolution ratio 17500, automatic growth control target are 1 × 105,
Maximum isolation time 50ms, maximum 10 ion scan secondary fragments, dynamic exclude.Liquid matter system is by Xcalibur 2.2
SP1.48 softwares control, and data acquisition and measured portions processing are operated by the software.Wherein Radix Curcumae Contained Serum sample is：
Give Radix Curcumae extract gavage to rat, successive administration 16 days, anesthesia, abdominal aortic blood, centrifugation, collection serum.It is wherein strongly fragrant
Golden method for preparing extractive is：Radix Curcumae is taken, is crushed, 40 mesh sieve is crossed, adds 10 times of amount water, first impregnates 0.5h, decocts 2 times, every time
45min, filtration, merge decocting liquid, be concentrated into 40ml to get；Wherein sample treatment is：200 μ L of Radix Curcumae Contained Serum are taken,
1300 μ L of chromatography methanol are added, fully shaking 20 seconds carries out albumen precipitation, centrifuges (12000r/min, 10min), nitrogen drying,
Add 120 μ L of methanol, centrifuge (12000r/min, 15 seconds), take supernatant to obtain the final product.
The application is using in Thermo UPLC-ITQ-0rbitrap high resolution mass spectrums analysis hand Radix Curcumae Contained Serum
Chemical composition is that research pharmacokinetics lays the first stone in next step.
A method of Radix Curcumae Contained Serum is analyzed using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums, it is special
Sign is, takes blank serum, 600 μ L of Radix Curcumae Contained Serum respectively, and 1800 μ L of chromatography methanol are added, centrifuge (12000r/min,
10min), N2 is dried up, and adds 100 μ L of methanol, is centrifuged (15000r/min, 15min), is taken supernatant sample introduction.Wherein, chromatographic condition is
Chromatographic condition：Chromatographic column：Therno Scientific Syncronic (C18,100 × 2.1mm, 1.7um), mobile phase：A is
Acetonitrile, B are 0.1% formic acid water, gradient elution program：0-20min, 10%A；20-45min, 10%~60%；45-50min,
60%~99%；50-60min, 99~10%.Flow velocity 0.2mL/min, column temperature：35 DEG C, sample size：3uL, Detection wavelength：
265nm；Mass Spectrometry Conditions：Scan mode：Negative ions scan respectively.Electron spray voltage：+ 5KV (cation), -5KV (bear from
Son)；Capillary temperature：350℃；Sheath gas：35psi；Secondary air speed：10psi；It winds up gas velocity：2psi.Scanning of the mass spectrum
Mode：Mode (is swept) entirely using FT Full Scan to be scanned, scanning range 50-1500Da；Resolution ratio：30000.MSN：It adopts
It is scanned with FT modes, resolution ratio：15000.The setting scanned using data dependence carries out collision-induced solution to most strong ion
From (CID), CID resolution widths are 2Da, and cracking energy is 35%.Wherein Radix Curcumae decocting liquid Contained Serum is that rat is taken continuously to fill
Stomach is administered 2 days, gives Radix Curcumae routine decocting liquid 5ml every time, 2 hours after the last administration, takes blood, centrifuges, supernatant is taken to obtain drug containing
Serum.
A method of Radix Curcumae is analyzed using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums, which is characterized in that
Radix Curcumae powder 1g is taken respectively, is placed in conical flask with stopper, 50ml methanol is added, and ultrasonic 30min crosses the miillpore filter mistake of 0.22um
After filter, direct injection analysis, wherein chromatographic condition is chromatographic condition：Chromatographic column：Thermo Scientific Syncronic
(C18,100 × 2.1mm, 1.7um), mobile phase：A is acetonitrile, and B is 0.1% formic acid water, gradient elution program：0-20min,
10%A；20-45min, 10%~60%；45-50min, 60%~99%；50-60min, 99~10%.Flow velocity 0.2mL/
Min, column temperature：35 DEG C, sample size：3uL, Detection wavelength：265nm；Mass Spectrometry Conditions：Scan mode：Negative ions scan respectively.Electricity
Spray voltage：+ 5KV (cation), -5KV (anion)；Capillary temperature：350℃；Sheath gas：35psi；Secondary air
Speed：10psi；It winds up gas velocity：2psi.Scanning of the mass spectrum mode：Mode (is swept) entirely using FT Full Scan to be scanned, and is scanned
Range 50-1500Da；Resolution ratio：30000.MSN：It is scanned using FT modes, resolution ratio：15000.It is swept using data dependence
The setting retouched carries out collision induced dissociation (CID) to most strong ion, and CID resolution widths are 2Da, and cracking energy is 35%.
Description of the drawings：
1 Radix Curcumae Contained Serum of attached drawing detects chromatogram-C1：Radix Curcumae extract radical anion peak figure (BPC), C2：Radix Curcumae water
Extract Contained Serum radical anion peak figure (BPC)；
2 Radix Curcumae Contained Serum of attached drawing detects chromatogram-D1：Radix Curcumae extract radical cation peak figure (BPC)；D2：Radix Curcumae water
Extract Contained Serum radical anion peak figure (BPC)
Specific implementation mode：
Specific experiment is as follows：
Using Thermo UPLC-LTQ-Orbitrap high resolution mass spectrums, to the Contained Serum of Radix Curcumae decocting liquid carried out at
Analysis, and analysis is carried out using turmeric medicinal substances extract, Radix Curcumae medicinal substances extract and is compareed as retinue.For curcumin at
Point, it is compared by accurate molecular weight and second order ms parses.
One, laboratory apparatus, reagent and method
1, instrument and reagent：UPLC-ITQ-Orbitrap (Thermo companies of the U.S.), supper-fast liquid chromatogram instrument
(Thermo Fisher Scientific, model DionexTMUltiMateTM300), quadrupole rod orbit ion trap mass spectrograph, first
Alcohol (chromatographic grade), acetonitrile (mass spectrum grade), high purity water, formic acid, glacial acetic acid, nitrogen.
2, chromatographic condition：Chromatographic condition：Chromatographic column：Thermo Scientific Syncronic (C18,100mm ×
2.1mm, 1.7 μm), mobile phase：A is the acetonitrile containing 0.1% formic acid (percent by volume), and B is to contain 0.1% glacial acetic acid (body
Product percentage) water, gradient elution program：0-20min, 10%A, 90%B；15-20min, 100%A, 0%B；20-25min,
30%A, 70%B.Flow velocity 0.30mL/min, column temperature：55 DEG C, sample size：1.5 μ L, Detection wavelength：265nm.
3, Mass Spectrometry Conditions：
Mass spectral analysis is using the quadrupole rod orbit ion trap mass spectrograph for being equipped with thermoelectricity esi ion source.Negative ions source electricity
Pressure is respectively 3.7ky and 3.5kv；Capillary heating temperature is：320℃；Sheath atmospheric pressure：30psi；Assist gas pressure power：10psi.
300 DEG C of volume heating evaporation temperature.Sheath gas and auxiliary gas are nitrogen.Collision gas is nitrogen, pressure 1.5mTorr.Level-one is swept
Retouching parameter is：Resolution ratio 7000, automatic growth control target are 1 × 106, maximum isolation time 50ms, mass-to-charge ratio scanning range
150-1500.Using data dependence scan pattern, design parameter is for compound component identification：Resolution ratio 17500, automatic gain control
Target processed is 1 × 105, maximum isolation time 50ms, maximum 10 ion scan secondary fragments, dynamic exclusion.Liquid matter system by
2.2 SP1.48 softwares of Xcalibur control, and data acquisition and measured portions processing are operated by the software.
4, sample-pretreating method：
It is prepared by Radix Curcumae medicinal substances extract：Radix Curcumae powder 1g is taken respectively, is placed in conical flask with stopper, 50ml methanol is added, and is surpassed
Sound 30min, after crossing the filtering with microporous membrane of 0.22um, direct injection analysis.
Radix Curcumae Contained Serum sample preparation：Give Radix Curcumae extract gavage to rat, successive administration 16 days, anesthesia, abdomen master
Serum is collected in arterial blood extracting, centrifugation.Wherein Radix Curcumae extract preparation method is：Radix Curcumae is taken, is crushed, 40 mesh sieve is crossed, adds 10 times of amounts
Water, first impregnates 0.5h, decocts 2 times, each 45min, and filtration merges decocting liquid, be concentrated into 40ml to get.
Sample treatment：200 μ L of Radix Curcumae Contained Serum are taken, 1300 μ L of chromatography methanol are added, fully shaking 20 seconds carries out albumen
Precipitation centrifuges (12000r/min, 10min), and nitrogen drying adds 120 μ L of methanol, centrifuges (12000r/min, 15 seconds), take supernatant
Liquid to obtain the final product.
5. sample analysis：The above-mentioned sample handled well is taken, according to above-mentioned chromatographic condition, Mass Spectrometry Conditions sample introduction.
Two, analysis result
1. Radix Curcumae extract and Contained Serum analysis result
1 Radix Curcumae extract of table and Contained Serum analysis result
**：What expression detected in Contained Serum
Brief summary：13 kinds of curcumin ingredients have been analyzed and identified in Radix Curcumae medicinal substances extract；From the drug containing blood of Radix Curcumae extract
3 compound Curcumin (curcumin), Dihydrocurcumin (double hydrogen curcumins), 5- have been analyzed and identified in clear
Hydroxy-1,7-bis (4-hydroxyphenyl) -3-heptanone (5- hydroxyls -1,7- bis- (4- hydroxy phenyls) -3- heptan
Ketone).Result above is to carry out Radix Curcumae pharmacokinetic in next step to offer reference.
Claims (10)
1. a kind of method for analyzing Radix Curcumae extract Contained Serum using UPLC-LTQ-Orbitrap, it is characterised in that take to be measured
Sample (Radix Curcumae extract Contained Serum) is carried out pre-treatment, is analyzed using UPLC-LTQ-Orbitrap, identify drug containing
Group in serum is divided into Curcumin (curcumin), Dihydrocurcumin (double hydrogen curcumins), 5-Hydroxy-1,7-bis
(4-hydroxyphenyl) -3-heptanone (bis- (4- the hydroxy phenyls) -3- heptanone of 5- hydroxyls -1,7-).
2. as described in claim 1 using the method for UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums, feature
For Radix Curcumae extract Contained Serum sample is：Give Radix Curcumae extract gavage to rat, successive administration 16 days, anesthesia, abdomen is actively
Arteries and veins takes blood, and serum is collected in centrifugation.Wherein Radix Curcumae extract preparation method is：Radix Curcumae is taken, is crushed, 40 mesh sieve is crossed, adds 10 times of amounts
Water, first impregnates 0.5h, decocts 2 times, each 45min, and filtration merges decocting liquid, be concentrated into 40ml to get.
3. using the side of UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums as described in claim any one of 1-2
Method, which is characterized in that the pre-treating method of sample to be tested is：200 μ L of Radix Curcumae extract Contained Serum are taken, chromatography methanol is added
1300 μ L, fully shaking 20 seconds carry out albumen precipitation, centrifuge (12000r/min, 10min), and nitrogen drying adds 120 μ L of methanol,
It centrifuges (12000r/min, 15 seconds), takes supernatant to obtain the final product.
4. special using the method for UPLC-LTQ-Orbitrap analysis Radix Curcumae Contained Serums as described in claim any one of 1-3
Sign is that chromatographic condition is：Chromatographic column：Thermo Scientific Syncronic (C18,100mm × 2.1mm, 1.7 μm), stream
Dynamic phase：A is the methanol containing 0.1% formic acid (percent by volume), and B is the water containing 0.1% glacial acetic acid (percent by volume), ladder
Spend elution program：0-20min, 10%A, 90%B；15-20min, 100%A, 0%B；20-25min, 30%A, 70%B.Flow velocity
0.30mL/min, column temperature：55 DEG C, sample size：1.5 μ L, Detection wavelength：265nm.
5. special using the method for UPLC-LTQ-Orbitrap analysis Radix Curcumae Contained Serums as described in claim any one of 1-4
Sign is Mass Spectrometry Conditions：Mass spectral analysis is using the quadrupole rod orbit ion trap mass spectrograph for being equipped with thermoelectricity esi ion source.It is positive and negative from
Component voltage is respectively 3.7kv and 3.5kv；Capillary heating temperature is：320℃；Sheath atmospheric pressure：30psi；Assist gas pressure power：
10psi.300 DEG C of volume heating evaporation temperature.Sheath gas and auxiliary gas are nitrogen.Collision gas is nitrogen, pressure 1.5mTorr,
Level-one sweep parameter is：Resolution ratio 7000, automatic growth control target are 1 × 106, maximum isolation time 50ms, mass-to-charge ratio sweeps
Range 150-1500 is retouched, using data dependence scan pattern, design parameter is for compound component identification：Resolution ratio 17500, automatically
Gain control targe is 1 × 105, maximum isolation time 50ms, maximum 10 ion scan secondary fragments, dynamic exclusion.
6. using the side of UPLC-LTQ-Orbitrap analysis Radix Curcumae extract Contained Serums as described in claim any one of 1-5
Method, it is characterized in that, liquid matter system is controlled by 2.2 SP1.48 softwares of Xcalibur, and data acquisition and measured portions processing are equal
It is operated by the software.
7. a kind of method for analyzing Radix Curcumae extract Contained Serum using UPLC-LTQ-Orbitrap high resolution mass spectrums, feature
It is, takes 200 μ L of Radix Curcumae Contained Serum, 1300 μ L of chromatography methanol are added, fully shaking 20 seconds carries out albumen precipitation, centrifugation
(12000r/min, 10min), nitrogen drying, adds 120 μ L of methanol, centrifuges (12000r/min, 15 seconds), takes supernatant sample introduction.Its
In, chromatographic condition is：Chromatographic condition：Chromatographic column：Thermo Scientific Syncronic (C18,100mm × 2.1mm,
1.7 μm), mobile phase：A is the methanol containing 0.1% formic acid (percent by volume), and B is to contain 0.1% glacial acetic acid (volume basis
Than) water, gradient elution program：0-20min, 10%A, 90%B；15-20min, 100%A, 0%B；20-25min, 30%A,
70%B.Flow velocity 0.30mL/min, column temperature：55 DEG C, sample size：1.5 μ L, Detection wavelength：265nm；Mass Spectrometry Conditions：Mass spectral analysis
Using the quadrupole rod orbit ion trap mass spectrograph for being equipped with thermoelectricity esi ion source.Negative ions source voltage be respectively 3.7kv and
3.5kv；Capillary heating temperature is：320℃；Sheath atmospheric pressure：30psi；Assist gas pressure power：10psi.Volume heating evaporation temperature
300℃.Sheath gas and auxiliary gas are nitrogen.Collision gas is nitrogen, pressure 1.5mTorr.Level-one sweep parameter is：Resolution ratio
7000, automatic growth control target is 1 × 106, maximum isolation time 50ms, mass-to-charge ratio scanning range 150-1500.Compound
Using data dependence scan pattern, design parameter is for component identification：Resolution ratio 17500, automatic growth control target are 1 × 105,
Maximum isolation time 50ms, maximum 10 ion scan secondary fragments, dynamic exclude.Liquid matter system is by Xcalibur 2.2
SP1.48 softwares control, and data acquisition and measured portions processing are operated by the software, wherein Radix Curcumae extract Contained Serum
Sample is：Give Radix Curcumae extract gavage to rat, successive administration 16 days, anesthesia, abdominal aortic blood, centrifugation, collection serum.
Wherein Radix Curcumae extract preparation method is：Radix Curcumae is taken, is crushed, 40 mesh sieve is crossed, adds 10 times of amount water, first impregnates 0.5h, decoct 2 times,
Each 45min, filtration, merge decocting liquid, be concentrated into 40ml to get；Wherein sample treatment is：Take Radix Curcumae Contained Serum
1300 μ L of chromatography methanol are added in 200 μ L, and fully shaking 20 seconds carries out albumen precipitation, centrifuges (12000r/min, 10min), nitrogen
Air-blowing is dry, adds 120 μ L of methanol, centrifuges (12000r/min, 15 seconds), takes supernatant to obtain the final product.
8. a kind of identifying Curcumin (curcumin) in Radix Curcumae extract Contained Serum, Dihydrocurcumin (double hydrogen ginger simultaneously
Flavine), 5-Hydroxy-1,7-bis (4-hydroxyphenyl) -3-heptanone (bis- (4- hydroxy benzenes of 5- hydroxyls -1,7-
Base) -3- heptanone) method, which is characterized in that using as described in claim any one of 1-7 using UPLC-LTQ-Orbitrap
The method for analyzing Radix Curcumae extract Contained Serum.
9. a kind of method that Radix Curcumae decocting liquid Contained Serum is analyzed using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums,
It is characterized in that, taking 600 μ L of Radix Curcumae decocting liquid Contained Serum, 1800 μ L of chromatography methanol are added, centrifuge (12000r/min,
10min), N2 is dried up, and adds 100 μ L of methanol, is centrifuged (15000r/min, 15min), is taken supernatant sample introduction.Wherein, chromatographic condition is
Chromatographic condition：Chromatographic column：Thermo Scientific Syncronic (C18,100 × 2.1mm, 1.7um), mobile phase：A is
Acetonitrile, B are 0.1% formic acid water, gradient elution program：0-20min, 10%A；20-45min, 10%~60%；45-50min,
60%~99%；50-60min, 99~10%.Flow velocity 0.2mL/min, column temperature：35 DEG C, sample size：3uL, Detection wavelength：
265nm；Mass Spectrometry Conditions：Scan mode：Negative ions scan respectively.Electron spray voltage：+ 5KV (cation), -5KV (bear from
Son)；Capillary temperature：350℃；Sheath gas：35psi；Secondary air speed：10psi；It winds up gas velocity：2psi.Scanning of the mass spectrum
Mode：Mode (is swept) entirely using FT Full Scan to be scanned, scanning range 50-1500Da；Resolution ratio：30000.MSN：It adopts
It is scanned with FT modes, resolution ratio：15000.The setting scanned using data dependence carries out collision-induced solution to most strong ion
From (CID), CID resolution widths are 2Da, and cracking energy is 35%.Wherein Radix Curcumae decocting liquid Contained Serum is that rat is taken continuously to fill
Stomach is administered 2 days, gives Radix Curcumae routine decocting liquid 5ml every time, 2 hours after the last administration, takes blood, centrifuges, supernatant is taken to obtain drug containing
Serum.
10. a kind of analyzing Radix Curcumae method using Thermo UPLC-ITQ-Orbitrap high resolution mass spectrums, which is characterized in that take strongly fragrant
600 μ L of golden sample are added 1800 μ L of chromatography methanol, centrifuge (12000r/min, 10min), and N2 dryings add 100 μ L of methanol, centrifuge
(15000r/min, 15min) takes supernatant sample introduction.Wherein, chromatographic condition is chromatographic condition：Chromatographic column：Thermo
Scientific Syncronic (C18,100 × 2.1mm, 1.7um), mobile phase：A is acetonitrile, and B is 0.1% formic acid water, gradient
Elution program：0-20min, 10%A；20-45min, 10%~60%；45-50min, 60%~99%；50-60min, 99~
10%.Flow velocity 0.2mL/min, column temperature：35 DEG C, sample size：3uL, Detection wavelength：265nm；Mass Spectrometry Conditions：Scan mode：It is positive and negative
Ion scans respectively.Electron spray voltage：+ 5KV (cation), -5KV (anion)；Capillary temperature：350℃；Sheath gas：
35psi；Secondary air speed：10psi；It winds up gas velocity：2psi.Scanning of the mass spectrum mode：Using the sides FT Full Scan (sweeping entirely)
Formula is scanned, scanning range 50-1500Da；Resolution ratio：30000.MSN：It is scanned using FT modes, resolution ratio：
15000.The setting scanned using data dependence carries out collision induced dissociation (CID) to most strong ion, and CID resolution widths are 2Da,
It is 35% to crack energy.Wherein turmeric sample is to be prepared with the following method：Turmeric, Radix Curcumae powder 1g are taken respectively, are placed in
In conical flask with stopper, 50ml methanol is added, ultrasonic 30min is spare after crossing the filtering with microporous membrane of 0.22um.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009236734A (en) * | 2008-03-27 | 2009-10-15 | Kinjo Gakuin | Method for separating and refining curcumins from turmeric powder (curcuma powder) by high speed counter flow chromatography |
| CN104914191A (en) * | 2015-06-29 | 2015-09-16 | 四川新绿色药业科技发展股份有限公司 | Method for simultaneously determining content of three effective components in turmeric products |
| CN105738546A (en) * | 2014-12-12 | 2016-07-06 | 桂林八加一药物研究股份有限公司 | Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof |
-
2018
- 2018-02-14 CN CN201810173021.1A patent/CN108562662A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009236734A (en) * | 2008-03-27 | 2009-10-15 | Kinjo Gakuin | Method for separating and refining curcumins from turmeric powder (curcuma powder) by high speed counter flow chromatography |
| CN105738546A (en) * | 2014-12-12 | 2016-07-06 | 桂林八加一药物研究股份有限公司 | Establishment method of curcuma aromatica medicine fingerprint map and the fingerprint map thereof |
| CN104914191A (en) * | 2015-06-29 | 2015-09-16 | 四川新绿色药业科技发展股份有限公司 | Method for simultaneously determining content of three effective components in turmeric products |
Non-Patent Citations (4)
| Title |
|---|
| HONGLIANG JIANG 等: "Use of liquid chromatography–electrospray ionization tandem mass spectrometry to identify diarylheptanoids in turmeric (Curcuma longa L.) rhizome", 《JOURNAL OF CHROMATOGRAPHY A》 * |
| 孙瑜 等: "姜黄、郁金、莪术鉴别及高效液相色谱法测定姜黄色素含量", 《实用中医内科杂志》 * |
| 胡晋卿 等: "HPLC-UV法测定大鼠血中姜黄素及一种新类似物的浓度", 《中药新药与临床药理》 * |
| 钟杰 等: "大鼠体内生地黄吸收成分分析及其药动学研究", 《药学学报》 * |
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