CN108562563A - Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ - Google Patents
Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ Download PDFInfo
- Publication number
- CN108562563A CN108562563A CN201810225012.2A CN201810225012A CN108562563A CN 108562563 A CN108562563 A CN 108562563A CN 201810225012 A CN201810225012 A CN 201810225012A CN 108562563 A CN108562563 A CN 108562563A
- Authority
- CN
- China
- Prior art keywords
- gold nano
- nano grain
- citrazinic acid
- gold
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000010931 gold Substances 0.000 title claims abstract description 51
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 229910052737 gold Inorganic materials 0.000 title claims abstract description 37
- CSGQJHQYWJLPKY-UHFFFAOYSA-N CITRAZINIC ACID Chemical compound OC(=O)C=1C=C(O)NC(=O)C=1 CSGQJHQYWJLPKY-UHFFFAOYSA-N 0.000 title claims abstract description 29
- 238000007306 functionalization reaction Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 238000012921 fluorescence analysis Methods 0.000 claims abstract description 5
- 239000000523 sample Substances 0.000 claims abstract description 5
- 239000011734 sodium Substances 0.000 claims description 16
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 15
- 229910052708 sodium Inorganic materials 0.000 claims description 15
- 239000001509 sodium citrate Substances 0.000 claims description 11
- FDWREHZXQUYJFJ-UHFFFAOYSA-M gold monochloride Chemical compound [Cl-].[Au+] FDWREHZXQUYJFJ-UHFFFAOYSA-M 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 8
- 238000002360 preparation method Methods 0.000 claims description 8
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000004737 colorimetric analysis Methods 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- GLUUGHFHXGJENI-UHFFFAOYSA-N Piperazine Chemical compound C1CNCCN1 GLUUGHFHXGJENI-UHFFFAOYSA-N 0.000 claims 4
- 244000248349 Citrus limon Species 0.000 claims 2
- 235000005979 Citrus limon Nutrition 0.000 claims 2
- 239000002253 acid Substances 0.000 claims 2
- 238000012800 visualization Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 25
- 229910052751 metal Inorganic materials 0.000 description 13
- 239000002184 metal Substances 0.000 description 13
- 150000001768 cations Chemical class 0.000 description 12
- 150000002500 ions Chemical class 0.000 description 12
- 235000011083 sodium citrates Nutrition 0.000 description 9
- RJHLTVSLYWWTEF-UHFFFAOYSA-K gold trichloride Chemical class Cl[Au](Cl)Cl RJHLTVSLYWWTEF-UHFFFAOYSA-K 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical class [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- 230000008033 biological extinction Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 238000000862 absorption spectrum Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011895 specific detection Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F1/00—Compounds containing elements of Groups 1 or 11 of the Periodic Table
- C07F1/005—Compounds containing elements of Groups 1 or 11 of the Periodic Table without C-Metal linkages
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/18—Metal complexes
- C09K2211/188—Metal complexes of other metals not provided for in one of the previous groups
Landscapes
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Materials Engineering (AREA)
- Plasma & Fusion (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention discloses the gold nano grains of citrazinic acid functionalization in detection Cr3+In application, the gold nano grain of the citrazinic acid functionalization can be used as fluorescence analysis detection Cr3+Fluorescence probe.The fluorescence probe is to Cr3+Specific good selectivity and anti-interference ability are, it can be achieved that Cr3+Visualization quickly detect.
Description
Technical field
The present invention relates to a kind of gold nano grains of citrazinic acid functionalization in detection Cr3+In application.
Background technology
Nano material refers at least one-dimensional being in three dimensions in nanoscale range (1nm~100nm) or by him
As basic unit constitute material.Since the 1970s comes out, since it has very unique physics, chemistry
And the properties such as biology, and as research hotspot in recent years.
Gold nano grain be even more because its have 1000 times of extinction coefficient higher than general dye molecule (13nm Au NPs'
Extinction coefficient is up to 2.7 × 108Mol/ (Lcm)), according to Beer-Lambert laws it is found that gold nano grain can reach
Detection limit be far below dye molecule.Furthermore since gold nano grain system has different color changes under different conditions,
Therefore gold nano grain occupies an important position in Visual retrieval.Since the Visual retrieval mechanism of gold nano grain is：
Red is presented in monodisperse gold particle in the solution, and when detected material is added, gold nano grain is assembled, to make
The plasmon coupling of intergranular changes, and red shift occurs for absorption peak, and the color of solution changes.Gold nano grain is in addition to tool
Have outside above-mentioned optical characteristics, surface is easy to be chemically modified the extensive use for being also gold nano grain in analysis detects
Provide convenient condition.Row such as gold nano grain surface can be realized by modifying small molecule, protein, polypeptide, DNA to not
With specific detection of target substance, including small molecule, heavy metal ion, protein, nucleic acid, tumour cell and pathogen etc..
Visible detection method based on gold nano grain does not depend on any large-scale instrument, and solution colour variation can be used as reading letter
Number, signal detection speed is fast, and material requested cost is more cheap, has been particularly suitable for quickly detection, Site Detection demand and item
Part falls behind the region for not having large-scale instrument relatively.
Invention content
It is an object of the invention to the present situations according to above-mentioned background technology, provide a kind of gold nano of citrazinic acid functionalization
Particle is in detection Cr3+In application.
In order to solve the above technical problem, the present invention provides the following technical solutions：
The gold nano grain of citrazinic acid functionalization is in detection Cr3+In application；Cr is detected using fluorescence analysis3+,
In, the gold nano grain of the citrazinic acid functionalization is detection Cr3+Fluorescence probe.
Preferably, the fluorescence analysis is optical colorimetry.The gold nano grain of the citrazinic acid functionalization is equally
Fluorescence probe be can be used as spectrophotometry Cr3+。
Preferably, the preparation process of the gold nano grain of the citrazinic acid functionalization includes：By gold chloride and citrazinic acid sodium
Mixed solution be heated to boiling, sodium citrate is then added, the gold nano grain of the citrazinic acid functionalization is obtained by the reaction.Its
In, citrazinic acid sodium is the ligand of gold chloride, and sodium citrate is reducing agent.
Preferably, the molar ratio of the gold chloride and citrazinic acid sodium is 1:1~30:1.Mole of gold chloride and citrazinic acid sodium
It is excessively more shallow or muddy than the too low or excessively high easy dispersion liquid color for making gold nano grain, the observation of color when visual colorimetric determination is influenced,
Therefore, more preferably molar ratio is 10:1.
Preferably, the molar ratio of the gold chloride and sodium citrate is 1:1.5~1:8.
Description of the drawings
Attached drawing is used to provide further understanding of the present invention, and a part for constitution instruction, the reality with the present invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the transmission electron microscope figure of the Au NPs of the present invention.As seen from the figure, gold nano grain is in monodisperse type,
Grain size is about 35nm.
Fig. 2 is the Au NPs+Cr of the present invention3+Transmission electron microscope figure.As seen from the figure, Cr is added3+Gold nano afterwards
Coherent condition is presented in grain.
Fig. 3 is the ion selectivity test chart of the present invention.Uv absorption spectra, which can be seen that, is added Cr3+Gold nano
Absorption peak of the particle at 525nm declines, and occurs new absorption peak at 700nm, and it is poly- that this also illustrates that gold nano grain occurs
Collection.Block diagram is clearly seen that Cr3+It is highly selective, wherein photo also will become apparent from the variation of color.
Fig. 4 is the ion interference test chart of the present invention.As seen from the figure, other 18 kinds of ion pair Cr3+Detection do not produce
Raw interference.
Fig. 5 is the sensitivity test figure of the present invention.
Specific implementation mode
Hereinafter, preferred embodiments of the present invention will be described with reference to the accompanying drawings, it should be understood that preferred reality described herein
Apply example only for the purpose of illustrating and explaining the present invention and is not intended to limit the present invention.
Embodiment 1
1) preparation of gold nano grain (Au NPs)：0.1mM 100mL gold chlorides and 0.1mM 100mL citrazinic acid sodium are mixed
It closes in 250mL round-bottomed flasks, is heated to boiling in a water bath, 20mg sodium citrates (Na is added immediately3C6H5O7), solution by
It is faint yellow to immediately become light gray, and baby pink is gradually become, stop heating after 20min, cooled to room temperature filters standby
With the transmission electron microscope figure of Au NPs is as shown in Figure 1.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the above-mentioned different metal cations of 20 μ L, find only have in 1mL centrifuge tubes
Added with Cr3+Solution colour change, and do not change added with the solution colour of other ions, as a result such as Fig. 3, Fig. 4
It is shown.Au NPs and Cr3+Transmission electron microscope figure in conjunction with after is as shown in Figure 2.
Comparison：The difference is that, be not added with ligand citrazinic acid sodium with the synthesis of above-mentioned Au NPs, above-mentioned most metals from
Son can make obtained Au NPs that color change occur, and cannot achieve to Cr3+Selective recognition.
The citrazinic acid sodium of the present invention is obtained by the way that citrazinic acid to be dissolved in sodium hydroxide solution.
Embodiment 2
1) preparation of gold nano grain：0.3mM 100mL gold chlorides and 0.1mM 100mL citrazinic acid sodium are blended in
It in 250mL round-bottomed flasks, is heated to boiling in a water bath, 20mg sodium citrates is added immediately, solution is immediately become by faint yellow
Black then becomes red, muddy stain, stops heating after 20min, cooled to room temperature filters spare.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the different metal cations of 20 μ L in 1mL centrifuge tubes, find only added with
Cr3+Solution grey is become from red immediately.And after crossing about 10min, added with Hg2+Solution colour slight change also has occurred,
But its variation is less apparent.
Embodiment 3
1) preparation of gold nano grain：0.3mM 100mL gold chlorides and 0.05mM 100mL citrazinic acid sodium are blended in
It in 250mL round-bottomed flasks, is heated to boiling in a water bath, 20mg sodium citrates is added immediately, solution is immediately become by faint yellow
Black then becomes red, muddy stain, stops heating after 20min, cooled to room temperature filters spare.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the different metal cations of 20 μ L in 1mL centrifuge tubes, find only added with
Cr3+Solution grey is become from red immediately.After crossing about 10min, variation is not observed added with the solution colour of other ions.
Embodiment 4
1) preparation of gold nano grain：0.3mM 100mL gold chlorides and 0.01mM 100mL citrazinic acid sodium are blended in
It in 250mL round-bottomed flasks, is heated to boiling in a water bath, 26mg sodium citrates is added immediately, solution is immediately become by faint yellow
Black then becomes baby pink, bright, stops heating after 20min, and cooled to room temperature filters spare.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the different metal cations of 20 μ L in 1mL centrifuge tubes, find only added with
Cr3+Solution grey is become from baby pink immediately.After crossing about 10min, the solution face for adding other metal ions is not observed
Color changes.
Embodiment 5
1) preparation of gold nano grain：0.3mM 100mL gold chlorides and 0.03mM 100mL citrazinic acid sodium are blended in
It in 250mL round-bottomed flasks, is heated to boiling in a water bath, 26mg sodium citrates is added immediately, solution is immediately become by faint yellow
Black then becomes pink, bright, stops heating after 20min, and cooled to room temperature filters spare.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the different metal cations of 20 μ L in 1mL centrifuge tubes, find only added with
Cr3+Solution grey is become from pink immediately, solution colour variation zone is shown clearly.After crossing about 10min, it is not observed and adds it
The solution colour of its metal ion changes.
Embodiment 6
1) preparation of gold nano grain：0.5mM 100mL gold chlorides and 0.05mM 100mL citrazinic acid sodium are blended in
(10 in 250mL round-bottomed flasks:1) it, is heated to boiling in a water bath, 26mg sodium citrates is added immediately, solution is by faint yellow vertical
Become black, then become red, color is deeper, stops heating after 20min, and cooled to room temperature filters spare.
2) ion detection：Prepare 10mL 10-3mol·L-119 kinds of different metal cation (Na+、K+、Ag+、NH4 +、
Mg2+、Ca2+、Cu2+、Co2+、Mn2+、Cd2+、Ni2+、Hg2+、Pb2+、Zn2+、Fe2+、Al3+、Fe3+、Cr3+、Cr6+).Take a series of phases
The above-mentioned Au NPs made of same volume sequentially add the different metal cations of 20 μ L in 1mL centrifuge tubes, find only added with
Cr3+Solution grey is become from red immediately.After crossing about 10min, it is not observed plus the solution colour of other metal ions is sent out
Changing.
3) a series of Cr of various concentrations is prepared3+Solution, successively from low concentration to high concentration be added dropwise Cr3+, while in purple
The variation tendency that its ultraviolet absorpting spectrum is observed under external spectrum, when collection of illustrative plates is not when changing or varying less, stopping is dripped
Add, obtains a figures in Fig. 5；Then two peak values and concentration are done into curve and obtains b figures；Then pass through fitting in the range of linearity
Curve is schemed to c.It can be seen that with Cr3+Concentration is incremented by, and absorption peak continuously decreases at 525nm, and the suction at 700nm
Peak is received to gradually increase.B, c figure are it can be seen that the linear correlation having had in the range of linearity, detection sensitivity reach 9nM.
Concentration unit mol/L of the present invention indicates that corresponding nM indicates nmol/L with M, μM expression μm ol/L, mM expression
mmol/L。
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention,
Although the present invention is described in detail referring to the foregoing embodiments, for those skilled in the art, still may be used
With technical scheme described in the above embodiments is modified or equivalent replacement of some of the technical features.
All within the spirits and principles of the present invention, any modification, equivalent replacement, improvement and so on should be included in the present invention's
Within protection domain.
Claims (7)
1. the gold nano grain of citrazinic acid functionalization is in detection Cr3+In application.
2. application according to claim 1, it is characterised in that：Cr is detected using fluorescence analysis3+, wherein the lemon piperazine
The gold nano grain of acid functionalization is detection Cr3+Fluorescence probe.
3. application according to claim 2, it is characterised in that：The fluorescence analysis is optical colorimetry.
4. application according to claim 1, it is characterised in that：The preparation of the gold nano grain of the citrazinic acid functionalization
Journey includes：The mixed solution of gold chloride and citrazinic acid sodium is heated to boiling, sodium citrate is then added, the lemon is obtained by the reaction
The gold nano grain of piperazine acid functionalization.
5. application according to claim 4, it is characterised in that：The molar ratio of the gold chloride and citrazinic acid sodium is 1:1~
30:1。
6. application according to claim 4, it is characterised in that：The molar ratio of the gold chloride and citrazinic acid sodium is 10:1.
7. application according to claim 4, it is characterised in that：The molar ratio of the gold chloride and sodium citrate is 1:1.5
~1:8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810225012.2A CN108562563A (en) | 2018-03-19 | 2018-03-19 | Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810225012.2A CN108562563A (en) | 2018-03-19 | 2018-03-19 | Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108562563A true CN108562563A (en) | 2018-09-21 |
Family
ID=63531779
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810225012.2A Pending CN108562563A (en) | 2018-03-19 | 2018-03-19 | Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108562563A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507454A (en) * | 2011-10-31 | 2012-06-20 | 中国科学院宁波材料技术与工程研究所 | Method for detecting trivalent chromium ions |
| CN102944551A (en) * | 2012-10-16 | 2013-02-27 | 中国科学院宁波材料技术与工程研究所 | Detection method of Cd2+ |
| WO2013159185A1 (en) * | 2012-04-24 | 2013-10-31 | University Health Network | Porphyrin-lipid stabilized nanoparticles for surface enhanced raman scattering based imaging |
| CN103852467A (en) * | 2014-03-13 | 2014-06-11 | 哈尔滨师范大学 | Chromium ion colorimetric detection probe and application method thereof |
| CN105372222A (en) * | 2014-08-22 | 2016-03-02 | 中国科学院生态环境研究中心 | Detection method of divalent heavy metal |
-
2018
- 2018-03-19 CN CN201810225012.2A patent/CN108562563A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507454A (en) * | 2011-10-31 | 2012-06-20 | 中国科学院宁波材料技术与工程研究所 | Method for detecting trivalent chromium ions |
| WO2013159185A1 (en) * | 2012-04-24 | 2013-10-31 | University Health Network | Porphyrin-lipid stabilized nanoparticles for surface enhanced raman scattering based imaging |
| CN102944551A (en) * | 2012-10-16 | 2013-02-27 | 中国科学院宁波材料技术与工程研究所 | Detection method of Cd2+ |
| CN103852467A (en) * | 2014-03-13 | 2014-06-11 | 哈尔滨师范大学 | Chromium ion colorimetric detection probe and application method thereof |
| CN105372222A (en) * | 2014-08-22 | 2016-03-02 | 中国科学院生态环境研究中心 | Detection method of divalent heavy metal |
Non-Patent Citations (6)
| Title |
|---|
| AI-JUNWANG等: "Sensitive and selective colorimetric detection of cadmium(II)using gold nanoparticles modified with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole", 《MICROCHIM ACTA》 * |
| NA CHEN等: "Colorimetric Detection of Cadmium Ions Using DL-Mercaptosuccinic Acid-Modified Gold Nanoparticles", 《NANOSCIENCE AND NANOTECHNOLOGY》 * |
| RUQAYA SHAIKH等: "2,3-Pyridine dicarboxylic acid functionalized gold nanoparticles: Insight into experimental conditions for Cr3+ sensing", 《SPECTROCHIMICA ACTA PART A: MOLECULAR AND BIOMOLECULAR SPECTROSCOPY》 * |
| SOUGATA SARKAR等: "A pH dependent Raman and surface enhanced Raman spectroscopic studies of citrazinic acid aided by theoretical calculations", 《SPECTROCHIMICA ACTA PART A: MOLECULAR AND BIOMOLECULAR SPECTROSCOPY》 * |
| 关桦楠等: "环糊精修饰金纳米粒子检测镉离子的研究", 《化学工程师》 * |
| 张付强等: "基于功能化金纳米粒子灵敏检测水溶液中的镉离子", 《广州化工》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wu et al. | Colorimetric determination of hexavalent chromium with ascorbic acid capped silver nanoparticles | |
| Xu et al. | Microwave-Assisted Rapid Synthesis of Amphibious Yellow Fluorescent Carbon Dots as a Colorimetric Nanosensor for Cr (VI). | |
| CN110118769B (en) | Gold nanoparticles for detecting heavy metal ions and preparation method thereof | |
| Alizadeh et al. | A review on gold nanoparticles aggregation and its applications | |
| US10928326B2 (en) | Selective colorimetric detection sensor and selective colorimetric detection method for detecting hexavalent chromium ions using size controlled label-free gold nanoparticles | |
| Xu et al. | Copper nanoclusters-based fluorescent sensor array to identify metal ions and dissolved organic matter | |
| Wang et al. | Carbon dot-and gold nanocluster-based three-channel fluorescence array sensor: Visual detection of multiple metal ions in complex samples | |
| Li et al. | Smartphone-assisted off─ on photometric determination of phosphate ion based on target-promoted peroxidase-mimetic activity of porous CexZr1-xO2 (x≥ 0.5) nanocomposites | |
| CN109777412B (en) | Double-emission fluorescent carbon dot and preparation method and application thereof | |
| CN101851677A (en) | Nucleic acid nano-gold biosensor used for detecting Hg2<+> | |
| Wang et al. | A single-nanozyme colorimetric array based on target-induced differential surface passivation for quantification and discrimination of Cl−, Br− and I− ions | |
| CN110907589B (en) | Visible Cu detection based on GQDs photocatalysis2+Method (2) | |
| Zeng et al. | Novel N, F co-doped carbon dots to detect sulfide and cadmium ions with high selectivity and sensitivity based on a “turn-off-on” mechanism | |
| CN105651744A (en) | Method for detecting Au3+ concentration | |
| Kong et al. | Dual-responsive ratiometric fluorescence detection of Ce4+ and ascorbic acid by regulating oxidase-mimicking activity of Ce4+-based nanocomplex | |
| Yang et al. | Portable intelligent paper-based sensors for rapid colorimetric and smartphone-assisted analysis of hydrogen peroxide for food, environmental and medical detection applications | |
| Huang et al. | Single-particle cadmium (Ⅱ) and chromium (Ⅲ) ions assay using GNP@ Ag core− shell nanoparticles with dark-field optical microscopy | |
| Kondekar et al. | Ultrasensitive, highly specific, colorimetric recognition of sulfide ions [S 2−] in aqueous media: applications to environmental analysis | |
| Feke et al. | Colorimetric detection of chromium (VI) via its instigation of oxidase-mimic activity of CuO | |
| CN104034711A (en) | Method for detecting potassium dichromate by utilizing graphene quantum dot probe | |
| Hua et al. | Quantitative colorimetric sensing of heavy metal ions via analyte-promoted growth of Au nanoparticles with timer or smartphone readout | |
| Ma et al. | An ultrahigh-resolution multicolor sensing platform via target-induced etching of gold nanorods for multi-colorimetric analysis of trace silver ions | |
| CN108562563A (en) | Application of the gold nano grain of citrazinic acid functionalization in detecting Cr3+ | |
| Shahamirifard et al. | Design and construction of a new optical solid-state mercury (II) sensor based on PVC membrane sensitized with colloidal carbon dots | |
| CN112717934A (en) | Attapulgite-loaded transition metal oxide composite material and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180921 |