CN108557997A - A kind of preparation of sludge lysate and its application in high-salt wastewater Anaerobic Treatment - Google Patents
A kind of preparation of sludge lysate and its application in high-salt wastewater Anaerobic Treatment Download PDFInfo
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- CN108557997A CN108557997A CN201810316668.5A CN201810316668A CN108557997A CN 108557997 A CN108557997 A CN 108557997A CN 201810316668 A CN201810316668 A CN 201810316668A CN 108557997 A CN108557997 A CN 108557997A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
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- Treatment Of Sludge (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention belongs to high-salt wastewater processing technology field, it is related to a kind of preparation of sludge lysate and its application in high-salt wastewater Anaerobic Treatment.By the secondary settling tank sludge natural subsidence of Sewage Disposal, supernatant, a concentration of 20 25g/L of conditioning of mud are removed；Born of the same parents are carried out brokenly to the microorganism in sludge with physical chemistry united method, release the Dissolve things inside of microorganism, sludge lysate is made.Sludge lysate is added in the biochemical reaction tank of high-salt wastewater, is sufficiently mixed under anaerobic condition uniformly.The present invention effectively shortens the period of the domestication of conventional salt-durable microbe, and the preparation method of sludge lysate is simple, energy consumption is low and pollution-free, and the minimizing and recycling for realizing sludge recycle, economical and reliable and stable.
Description
Technical field
The invention belongs to high-salt wastewater processing technology field, it is related to a kind of preparation of sludge lysate and its in high-salt wastewater
The application of Anaerobic Treatment.
Background technology
Referred to as high-salt wastewater when inorganic salts mass percent is more than 1% in waste water, is a great problem of field of waste water treatment.
Many links will produce a large amount of high salinity waste water in industrial production, such as marine products processing process, printing and dyeing, oil recovery, papermaking, chemical industry
And seawater utilization etc., and in these waste water other than the salt containing high concentration, usually also organic dirt containing high concentration
Object is contaminated, it is saline and alkaline such as soil if many problem of environmental pollutions will be caused in these untreated direct discharging of waste water to environment
Change, the eutrophication etc. of water body, therefore a kind of cost-effective becoming for processing means processing high-salt wastewater is taken to study at present
The task of top priority of person and engineer.With general physico-chemical process such as advanced oxidation, ion exchange, multiple-effect evaporation, reverse osmosis
Etc. techniques be successfully applied to the processing of high-salt wastewater, but the running of the high-tech of these complexity and high cost limits
The extensive use of these technologies, and bioanalysis is because with good performance driving economy and non-secondary pollution, being presently believed to be makes
The most efficient method of organics removal.Compared with Aerobic Process for Treatment, Anaerobic Treatment is in organics removal and life in bioprocess
There is significant advantage in the recycling of object fuel gas, but mesohaline drastically increase of high-salt wastewater causes microbial cell osmotic pressure
The plasmolysis and death of cell are increased and then caused, inhibits the activity of 50% methanogen, therefore with traditional anaerobic organism
It is very difficult that processing method, which removes processing high-salt wastewater, and with Biochemical method high-salt wastewater, there is still a need for more complicated processing sides
Formula.
When microorganism is by salinity shock, normal metabolism is suppressed, and microorganism can generally pass through two kinds
Salinity mechanism copes with extraneous hyperosmosis and ionic strength, i.e., cell inner salt mechanism and organic matter permeate mechanism.Organic matter oozes
Saturating mechanism refers to accumulation in the cell (synthesis is absorbed from the external world) small-molecule substance and, come the extraneous hypertonic environment that contends with, has now been found that
Permeation protective agent mainly have glycine, proline, glycine betaine, trehalose and potassium ion etc., but the cost purified is higher, no
It is suitble to industrialized large-scale application.The effect for the permeation protective agent usually extracted from biology is better than the protective agent of synthesis,
Sludge mainly by microorganism group at, can by physico-chemical process crack microbial cell, release many nutriments,
Including amino acid, nucleotide, monosaccharide and vitamin etc. and their polymer, such as protein, DNA, RNA and its polysaccharide
Deng, these small molecule nutriments not only can this microorganism recycle for growing, the case where external environment changes suddenly
Under, it can be entered through cell membrane into the cell to maintain the balance of intraor extracellular osmotic pressure, avoid losing activity because of dehydration.
Therefore compared with other physical chemistry, strengthen the Anaerobic Treatment process of high-salt wastewater by sludge lysate, be not only pair
A kind of recycling of residual active sludge, and it is economical pollution-free.
Invention content
The unresolved above problem uses sludge lysate as permeation protective agent, effective solution the present invention provides a kind of
Facultative Halophiles acclimation periods is long, microorganism plasmolysis and death problem, at the same to excessive excess sludge realize minimizing and
The utilization of recycling provides a kind of method of effective enhanced biological processing for processing high-salt wastewater.The present invention is using surplus
Remaining sludge prepares sludge lysate, is added in the biological treatment process of high-salt wastewater as permeation protective agent, realizes to high salt
The reinforcing of anaerobic waste water biological treatment methane phase.
Technical scheme of the present invention：
A kind of preparation method of sludge lysate, steps are as follows：
By secondary settling tank sludge natural subsidence, supernatant, a concentration of 20-25g/L of conditioning of mud are removed；Combined with physical chemistry
Method carries out brokenly born of the same parents to the microorganism in sludge, and the united method of physical chemistry of use is the method connection added with ultrasound and alkali
Close, first by sludge under conditions of acoustic density is 3w/ml ultrasound 20min, then in the ratio of 1g/g (TS/NaOH) add alkali,
5h is mixed.The Dissolve things inside of microorganism is released, sludge lysate is made.
Wherein, TS is total solid, refers to the total amount that sludge is evaporated to all remaining solids of constant weight at 105 DEG C, including sludge
In suspended matter, colloid substances and dissolved matter, wherein existing organic matter also organic/inorganic substance.
A kind of sludge lysate is in the application of high-salt wastewater Anaerobic Treatment, and steps are as follows：
Sludge lysate is added in the biochemical reaction tank of high-salt wastewater, is sufficiently mixed under anaerobic condition uniformly；Control
Sludge concentration in biochemical reaction tank is 2~3g/L, volume and the mud in biochemical reaction tank after addition lysate of sludge lysate
The total volume ratio of aqueous mixtures is that 0.05~0.15, COD is 1000mg/L~1500mg/L, and reaction temperature is 30~35 DEG C, pH
Be 7.0~8.0, hydraulic detention time be 18~for 24 hours.
The method of the present invention beneficial effect：The present invention effectively shortens the period of the domestication of conventional salt-durable microbe,
The preparation method of sludge lysate is simple, energy consumption is low and pollution-free, and the minimizing and recycling for realizing sludge recycle, economical
And it is reliable and stable.
Description of the drawings
Fig. 1 is passage at any time, the yield schematic diagram of methane under the salinity of 35g/L；
Fig. 2 is passage at any time, the yield schematic diagram of methane under the salinity of 40g/L；
Fig. 3 is passage at any time, the yield schematic diagram of methane under the salinity of 45g/L；
Fig. 4 is passage at any time, the yield schematic diagram of methane under the salinity of 50g/L.
Specific implementation mode
Below in conjunction with attached drawing and technical solution, the specific implementation mode that further illustrates the present invention.
Embodiment 1
(1) preparation of sludge lysate
The secondary settling tank sludge for removing supernatant after settling is taken, its a concentration of 20g/L is adjusted, it is small that 1 is cracked under conditions of 100 DEG C
When, alkali NaOH is added according still further to the ratio of 0.7g/g (TS/NaOH), mixture is fully anti-in 35 DEG C, the shaking table of 150rpm
Smudge cells are answered, after 5 hours, are centrifuged 20 minutes in 10000r/min, and collect supernatant, as sludge lysate.
(2) anaerobic fermentation stage of sludge lysate intensive treatment high-salt wastewater
The high-salt wastewater fetched from factory is diluted, it is respectively 35g/L, 40g/L, 45g/L, 50g/ to make its salt content
L, in the anaerobic fermentation stage of processing high-salt wastewater, the sludge lysate that step 1 is obtained is added in reaction bulb, keeps anaerobism ring
Border, dirty a concentration of 2.5gVSS/L, PH=7.0, COD 1000, are then added sludge lysate and total volume ratio are in bottle
0.05, the domestication of sludge is carried out after mixing in 35 DEG C, the shaking table of 150rpm.The continuous production for cultivating and detecting anaerobic methane production
Amount.
Embodiment 2
(1) preparation of sludge lysate
Its a concentration of 25g/L of the secondary settling tank sludge conditioning of supernatant, in Ultrasonic Cell Disruptor, the sound of 3w/ml are removed after taking sedimentation
Ultrasound 20 minutes, adds alkali NaOH according still further to the ratio of 1g/g (TS/NaOH), pours the mixture into anaerobism bottle under energy density,
Smudge cells are fully reacted in 35 DEG C, the shaking table of 150rpm, after 5 hours, are centrifuged 20 minutes in 10000r/min, and collect
Clear liquid, as sludge lysate.
(2) sludge lysate intensive treatment printing and dyeing high-salt wastewater
The high-salt wastewater fetched from printworks is diluted, make its salt content be respectively 35g/L, 40g/L, 45g/L,
Sludge lysate and sludge that step 1 obtains are added in high-salt wastewater by 50g/L together, holding anaerobic environment, dirty dense in bottle
Degree is 2.5gVSS/L, PH=7.0~8.0, COD 1000, and it is 0.1 that sludge lysate and total volume ratio, which is then added, mixing
The domestication of sludge is carried out in 35 DEG C, the shaking table of 150rpm afterwards.The continuous yield for cultivating and detecting anaerobic methane production.
Experimental result is as shown in Figs 1-4, and reinforcing group is to add the system of lysate, and control group is the body for being not added with lysate
System shows in figure during continuous culture, under different salinity, the methane production of sludge lysate fortification system
All it is apparently higher than the control systems of no sludge lysate.
Claims (4)
1. a kind of preparation method of sludge lysate, which is characterized in that steps are as follows：
By secondary settling tank sludge natural subsidence, supernatant, a concentration of 20-25g/L of conditioning of mud are removed；With physical chemistry integrated processes
Born of the same parents are carried out brokenly to the microorganism in sludge, release the Dissolve things inside of microorganism, sludge lysate is made.
2. the preparation method of sludge lysate according to claim 1, which is characterized in that the physical chemistry of use is united
Method is the method joint added with ultrasound and alkali.
3. the preparation method of sludge lysate according to claim 2, which is characterized in that the physical chemistry of use is united
Method is as follows：First by sludge under conditions of acoustic density is 2w/ml~4w/ml ultrasound 10min~30min；Again
NaOH is added for 0.8~1.2 by the mass ratio of TS and NaOH, 3~6h is mixed.
4. a kind of sludge lysate is in the application of high-salt wastewater Anaerobic Treatment, which is characterized in that steps are as follows：
Sludge lysate is added in the biochemical reaction tank of high-salt wastewater, is sufficiently mixed under anaerobic condition uniformly；Control is biochemical
Sludge concentration in reaction tank is 2~3g/L, and the volume of sludge lysate and muddy water in biochemical reaction tank after addition lysate are mixed
The total volume ratio for closing object is that 0.05~0.15, COD is 1000mg/L~1500mg/L, and reaction temperature is 30~35 DEG C, pH 7.0
~8.0, hydraulic detention time be 18~for 24 hours.
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Citations (9)
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|---|---|---|---|---|
| JPH07328696A (en) * | 1994-06-02 | 1995-12-19 | Kajima Corp | Treating method of surplus sludge |
| CN101565262A (en) * | 2009-06-04 | 2009-10-28 | 同济大学 | Combined pre-treatment method for improving sludge disintegration effect and strengthening subsequent anaerobic digestion |
| CN104628234A (en) * | 2015-02-03 | 2015-05-20 | 同济大学 | Method for reducing resistance gene release in anaerobic sludge treatment process |
| CN104909455A (en) * | 2015-05-24 | 2015-09-16 | 北京工业大学 | Method for relieving anaerobic ammonium oxidation system salinity shock with betaine as additive |
| CN105330114A (en) * | 2015-10-22 | 2016-02-17 | 天津大学 | Pretreatment method of promoting anaerobic fermentation of high-density sludge through alkaline-hydrolysis coupling ultrasonic processing |
| CN105585231A (en) * | 2014-11-04 | 2016-05-18 | 中国石油化工股份有限公司 | Sludge reduction method with ultrasonic and alkali treatment |
| CN105621826A (en) * | 2016-01-19 | 2016-06-01 | 辽宁大学 | Method for promoting anaerobic digestion of residual activated sludge to generate methane by pretreatment combined electrochemical technology |
| CN105836994A (en) * | 2016-05-27 | 2016-08-10 | 轻工业环境保护研究所 | Intermediate-enhanced sludge digestion process |
| CN107434341A (en) * | 2017-06-16 | 2017-12-05 | 中国科学院生态环境研究中心 | A kind of method of sludge high efficient resourcing |
-
2018
- 2018-04-04 CN CN201810316668.5A patent/CN108557997A/en not_active Withdrawn
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07328696A (en) * | 1994-06-02 | 1995-12-19 | Kajima Corp | Treating method of surplus sludge |
| CN101565262A (en) * | 2009-06-04 | 2009-10-28 | 同济大学 | Combined pre-treatment method for improving sludge disintegration effect and strengthening subsequent anaerobic digestion |
| CN105585231A (en) * | 2014-11-04 | 2016-05-18 | 中国石油化工股份有限公司 | Sludge reduction method with ultrasonic and alkali treatment |
| CN104628234A (en) * | 2015-02-03 | 2015-05-20 | 同济大学 | Method for reducing resistance gene release in anaerobic sludge treatment process |
| CN104909455A (en) * | 2015-05-24 | 2015-09-16 | 北京工业大学 | Method for relieving anaerobic ammonium oxidation system salinity shock with betaine as additive |
| CN105330114A (en) * | 2015-10-22 | 2016-02-17 | 天津大学 | Pretreatment method of promoting anaerobic fermentation of high-density sludge through alkaline-hydrolysis coupling ultrasonic processing |
| CN105621826A (en) * | 2016-01-19 | 2016-06-01 | 辽宁大学 | Method for promoting anaerobic digestion of residual activated sludge to generate methane by pretreatment combined electrochemical technology |
| CN105836994A (en) * | 2016-05-27 | 2016-08-10 | 轻工业环境保护研究所 | Intermediate-enhanced sludge digestion process |
| CN107434341A (en) * | 2017-06-16 | 2017-12-05 | 中国科学院生态环境研究中心 | A kind of method of sludge high efficient resourcing |
Non-Patent Citations (1)
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| 雷太平: ""污泥裂解液强化高盐偶氮染料废水脱色研究"", 《中国优秀硕士学位论文全文数据库（电子期刊）工程科技Ⅰ辑》 * |
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Application publication date: 20180921 |