CN108552223A - A kind of liquid bacteria suspension and its application containing bacillus - Google Patents
A kind of liquid bacteria suspension and its application containing bacillus Download PDFInfo
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- CN108552223A CN108552223A CN201810044252.2A CN201810044252A CN108552223A CN 108552223 A CN108552223 A CN 108552223A CN 201810044252 A CN201810044252 A CN 201810044252A CN 108552223 A CN108552223 A CN 108552223A
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- Prior art keywords
- bacillus
- liquid
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- bacterium
- bacterium suspension
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
Abstract
The liquid bacteria suspension containing bacillus that the invention discloses a kind of, it includes general bacillus liquid bacterium suspension, the bacillus liquid bacterium suspension suitable for organic agriculture.It include mainly agriculture breast 34：2.68%~4.18%；D‑425：2.32%~2.56%；OP‑10：1.78%~2.06%；Potassium sorbate：0.48%~0.65%；Glycerine：4.96%~6.18%；Xanthans：0.16%~0.22%；Surplus group is divided into fermentation of bacillus liquid.The fermentation of bacillus liquid refers to the liquid bacteria suspension containing TB1340, TB1344, TB1358.Bacillus liquid bacterium suspension of the present invention is succeeded in developing, not only make bacillus preparation chemical industry skill simpler, cost is saved, improve bacillus preparation efficiency, more importantly improve the stability of bacterium suspension, the promotion and application of excellent Biocontrol Bacillus bacterial strain are accelerated, the use of chemical agent and chemical fertilizer is reduced.
Description
Technical field
The present invention relates to research of plant disease technical field, specifically a kind of liquid bacteria suspension containing bacillus and
It is applied.
Background technology
Plant disease causes great economic loss to the harm getting worse of agricultural production.Currently, mainly by adopting
Prevention and control were carried out with chemical agent, and the long-time service of chemical agent, pesticide residue and water, native environmental pollution can be caused, is destroyed
The ecological balance threatens the serious consequences such as human security.With the enhancing of people's environmental consciousness, food-safe attention and agriculture
The requirement of industry sustainable development, biological control plant disease are increasingly becoming current research and the hot spot of exploitation.Bacillus is
More extensive biocontrol microorganisms resource, including bacillus subtilis, bacillus amyloliquefaciens, waxy gemma bar are studied and applied to one kind
Bacterium etc..Because it is with good high temperature resistant, acidproof, salt resistance ability, to plant, human body and environmentally friendly, metabolite tool
There is the effects that certain sterilization, growth-promoting, do not cause the disease resistance of plant, and be easy to formulation, is widely used in agricultural production.
Bacillus preparation is mainly reflected in bio-bacterial manure, composite fertilizer, biological pesticide addition etc., system both at home and abroad at present
It is more complicated to make technique, cost is higher, is unfavorable for the promotion and application of biocontrol microorganisms.The research of bacillus liquid bacterium suspension is also located
In bottleneck period, for liquid bacteria suspension at being grouped as also in the exploratory stage, the stability of bacterium suspension is difficult manipulation, carries out bacillus
The research significance of liquid bacteria suspension is great.Bacillus liquid bacterium suspension is succeeded in developing, and bacillus preparation chemical industry is not only made
Skill is simpler, has saved cost, improves bacillus preparation efficiency, it is often more important that accelerates excellent biological and ecological methods to prevent plant disease, pests, and erosion gemma bar
The promotion and application of bacteria strain reduce the use of chemical agent and chemical fertilizer.
Invention content
To achieve the above object, the liquid bacteria suspension containing bacillus that the invention discloses a kind of, it is characterised in that it is wrapped
General bacillus liquid bacterium suspension, the bacillus liquid bacterium suspension suitable for organic agriculture are included, wherein general bacillus
Liquid bacteria suspension is made of following weight percent composition;
Agriculture breast 34：2.68%~4.18%
D-425：2.32%~2.56%
OP-10：1.78%~2.06%
Potassium sorbate：0.48%~0.65%
Glycerine：4.96%~6.18%
Xanthans：0.16%~0.22%
Surplus group is divided into fermentation of bacillus liquid；
Bacillus liquid bacterium suspension suitable for organic agriculture
Organosilicon：2.83%~3.62%
Potassium sorbate：0.48%~0.65%
Sucrose：3.54%~4.26%
Xanthans：0.16%~0.22%
Surplus group is divided into fermentation of bacillus liquid；
The fermentation of bacillus liquid refers to the liquid bacteria suspension of TB1340, TB1344, TB1358；About TB1340,
TB1344, TB1358 bacterial strain information, referring to Shandong agricultural sciences, volume 27, the third phase, page 26, phytophthora blight of pepper antagonism is thin
Screening, identification and the control effect testing of bacterium.
The present invention further discloses the preparation methods of the bacterium suspension of liquid containing bacillus, it is characterised in that by following step into
Row：
It is prepared by general bacillus liquid bacterium suspension：
（1）Prepare Biocontrol Bacillus zymotic fluid；
（2）Each ingredient is mixed according to following ratio：
Agriculture breast 34：2.68%~4.18%
D-425：2.32%~2.56%
OP-10：1.78%~2.06%
Potassium sorbate：0.48%~0.65%
Glycerine：4.96%~6.18%
Xanthans：0.16%~0.22%
Remaining group is divided into fermentation of bacillus liquid；
（3）By mixed liquor turbula shaker, with the rotating speed of 200 rmp/min, continuously concussion mixes well, and bacterium suspension can be observed
Precipitation is not generated, it is evenly dispersed, place room temperature preservation；
It is prepared by the bacillus liquid bacterium suspension suitable for organic agriculture：
（1）Prepare Biocontrol Bacillus zymotic fluid；
（2）Each ingredient is mixed according to following ratio：
Organosilicon：2.83%~3.62%
Potassium sorbate：0.48%~0.65%
Sucrose：3.54%~4.26%
Xanthans：0.16%~0.22%
Remaining group is divided into fermentation of bacillus liquid；
（3）By mixed liquor turbula shaker, with the rotating speed of 200 rmp/min, continuously concussion mixes well, and bacterium suspension can be observed
It does not precipitate, it is evenly dispersed, place room temperature preservation.
Biocontrol Bacillus zymotic fluid preparation method：By taking biocontrol bacterial strain TB1340 as an example（Common zymotic fluid preparation method）：
（1）Solid LB media is prepared：10g tryptones, 10g sodium chloride, 5g beef extract powder, 20g agar powders, distilled water
It is settled to 1 liter, 100 milliliters of triangular flasks dispense, 121 DEG C, moist heat sterilization 30 minutes.
LB liquid medium is prepared：10g tryptones, 10g sodium chloride, 5g beef extract powder, distilled water are settled to 1 liter, 100 millis
Triangular flask packing is risen, 5 milliliters of test tubes dispense, 121 DEG C, moist heat sterilization 30 minutes.
（2）LB solid plate activated strains TB1340 take activated single bacterium colony to be inoculated in 5 milliliters of liquid LB culture mediums,
160rpm is incubated overnight, as TB1340 seed liquors.
（3）The seed liquor that will be prepared, 1/1000 is inoculated in 100 milliliters of liquid LB culture mediums, 160rpm, cultivates 48-72h,
The TB1340 zymotic fluids as prepared.
The present invention further discloses application of the liquid bacteria suspension containing bacillus in terms of improving bacterium suspension stability.It is real
It tests result and shows the bacterium suspension viable bacteria clump count containing different strains 3d, 30d, 90d after the preparation of bacterium suspension, obviously do not become
Change, still maintains biological and ecological methods to prevent plant disease, pests, and erosion activity, and deposited phenomenon does not occur in bacterium suspension, stability is good.
General bacillus liquid bacterium suspension formula disclosed by the invention and the Biocontrol Bacillus liquid suitable for organic agriculture
Bacterium suspension formula, two kinds of formulas can be used as Biocontrol Bacillus liquid bacteria suspension to make formula.General Biocontrol Bacillus
Bacterium suspension made by liquid bacteria suspension formula can be used in any agricultural production, be suitable for the Biocontrol Bacillus of organic agriculture
Liquid bacteria suspension formula is on the basis of general formulation, it is contemplated that organic agriculture it is prevailing, use organosilicon to replace other
Dispersant adjusts density using sucrose, harmless to agricultural production and human body.
Specific implementation mode
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, implement below in conjunction with the present invention
Example, technical scheme in the embodiment of the invention is clearly and completely described.It should be noted that：
Agriculture breast 34 of the present invention refers to No. 34 emulsifiers of pesticide, has the works such as good dispersion, wetting, infiltration, adhesion
With purchase is in modest and amiable Chemical Co., Ltd. of Nantong City；
D-425 refers to the dispersant used in aqueous suspension agent and dry dosage form, buys in Nanjing Jie Run Science and Technology Ltd.s；
OP-10 is a kind of emulsifier, has good emulsification, moistening, diffusion, characteristic of solubilizing, purchase is in Tianjin Tianjin hundred along examination
Agent Co., Ltd；
Potassium sorbate is a kind of common food preservative, and purchase is in Tianjin Tianjin hundred along reagent Co., Ltd；Xanthans, tool
There are many functions, can be used as emulsifier, stabilizer, gelling thickener, size, film forming agent etc., are widely used in national warp
It helps each field, purchase is in Tianjin Tianjin hundred along reagent Co., Ltd；Glycerine and sucrose purchase are limited along reagent in Tianjin Tianjin hundred
Company；Organosilicon assists Pesticide use usually as auxiliary agent, has extraordinary dispersibility, buys in Weifang City Wo Fengsheng
Object fertilizer Co., Ltd；
Fermentation of bacillus liquid in the present invention is not directed to specific Biocontrol Bacillus, the zymotic fluid of any bacillus
It can be with.Such as bacillus subtilis, bacillus amyloliquefaciens, bacillus cereus etc., typically TB1340,
TB1344、TB1358.About TB1340, TB1344, TB1358 bacterial strain information, referring to Shandong agricultural sciences, volume 27, third
Phase, page 26, screening, identification and the control effect testing of phytophthora blight of pepper antagonistic bacterium.Bacterial strain TB1340, TB1344, TB1358 are
Agricultural microbial fermentation research department of Tianjin Plant Protection Institute has bacterial strain by oneself, belongs to bacillus, used in research
Bacterial strain, can externally provide free, only pursue fundamental studies use, it is impossible to be used in the production of microorganism formulation.
Embodiment 1
（1）Biocontrol Bacillus zymotic fluid preparation method：By taking biocontrol bacterial strain TB1358 as an example.（Common zymotic fluid preparation side
Method）：
1）Solid LB media is prepared：10g tryptones, 10g sodium chloride, 5g beef extract powder, 20g agar powders, distilled water are fixed
Hold to 1 liter, 100 milliliters of triangular flasks dispense, 121 DEG C, moist heat sterilization 30 minutes.
LB liquid medium is prepared：10g tryptones, 10g sodium chloride, 5g beef extract powder, distilled water are settled to 1 liter, 100 millis
Triangular flask packing is risen, 5 milliliters of test tubes dispense, 121 DEG C, moist heat sterilization 30 minutes.
2）LB solid plate activated strains TB1358 take activated single bacterium colony to be inoculated in 5 milliliters of liquid LB culture mediums,
160rpm is incubated overnight, as TB1340 seed liquors.
3）The seed liquor that will be prepared, 1/1000 is inoculated in 100 milliliters of liquid LB culture mediums, 160rpm, cultivates 48-72h, i.e.,
For the TB1358 zymotic fluids prepared.
（2）Each ingredient is mixed according to following ratio：
Organosilicon：3%
Potassium sorbate：0.6%
Sucrose：4%
Xanthans：0.18%
Remaining group is divided into containing TB1358 fermentation of bacillus liquid；
By mixed liquor turbula shaker, with the rotating speed of 200 rmp/min, continuously concussion mixes well, and bacterium suspension can be observed not
Precipitation, it is evenly dispersed, place room temperature preservation.
Embodiment 2
The stability test of 3 kinds of bacterium suspensions containing different strains：
Bacterium suspension Detection of Stability
Bacterium suspension Detection of Stability is the results show that viable bacteria clump count 3d, 30d, 90d after the preparation of bacterium suspension, without significant change,
Biological and ecological methods to prevent plant disease, pests, and erosion activity is still maintained, and deposited phenomenon does not occur in bacterium suspension, stability is good.
Embodiment 3
General bacillus liquid bacterium suspension containing bacterial strain TB1340 prevents Phytophthora capsici disease effect measuring
Method：Indoor biometrics experiment
Step：
1. after selection pepper seed of uniform size is sterilized, impregnating 4 h with aqua sterilisa, being sowed to dew in 28 DEG C of vernalization
In vain；
2. with the general bacillus liquid bacterium suspension seed soaking 45min containing bacterial strain TB1340；
3. being put into 4 seeds in the nutritive cube equipped with sterilized soil, stay two plants of growing ways uniform when seedling grew to the 3-4 leaf phases
Seedling handles 20 basins；
4. after Phytophthora capsici pathogen is cultivated 10d with CA culture mediums at 25 DEG C, zoospore is induced, it will with sterile water
Zoospore concentration is adjusted to 1.0*105Cfu/ml is spare；
5. 5ml zoospore suspensions are injected plant root at plant 3cm；
6. experiment plant is positioned over 28 DEG C of illumination box cultures, plant is investigated after 7,14,21d and is wilted（Diseased plant
Number）Situation.
Conclusion:General bacillus liquid bacterium suspension prevention Phytophthora capsici disease 7 containing bacterial strain TB1340,14,21d prevent relatively
Effect reaches 100%, 92%, 88%, and control effect is preferable.
Embodiment 4
The bacillus liquid bacterium suspension suitable for organic agriculture containing bacterial strain TB1344 prevents Phytophthora capsici disease effect measuring
Method：Indoor biometrics experiment
Step：
1. after selection pepper seed of uniform size is sterilized, impregnating 4 h with aqua sterilisa, being sowed to dew in 28 DEG C of vernalization
In vain；
2. with the bacillus liquid bacterium suspension seed soaking 45min suitable for organic agriculture containing bacterial strain TB1344；
3. being put into 4 seeds in the nutritive cube equipped with sterilized soil, stay two plants of growing ways uniform when seedling grew to the 3-4 leaf phases
Seedling handles 20 basins；
4. after Phytophthora capsici pathogen is cultivated 10d with CA culture mediums at 25 DEG C, zoospore is induced, it will with sterile water
Zoospore concentration is adjusted to
5. 5ml zoospore suspensions are injected plant root at plant 3cm；
6. experiment plant is positioned over 28 DEG C of illumination box cultures, plant is investigated after 7,14,21d and is wilted（Diseased plant
Number）Situation.
Conclusion:General bacillus liquid bacterium suspension prevention Phytophthora capsici disease 7d, 14d, 21d containing bacterial strain TB1344 are opposite
Preventive effect reaches 88%, 90%, 78%, and control effect is preferable.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although
Present invention has been described in detail with reference to the aforementioned embodiments, for those skilled in the art, still can be right
Technical solution recorded in foregoing embodiments is modified or equivalent replacement of some of the technical features.It is all
Within the spirit and principles in the present invention, any modification, equivalent replacement, improvement and so on should be included in the protection of the present invention
Within the scope of.
Claims (3)
1. a kind of liquid bacteria suspension containing bacillus, it is characterised in that it includes general bacillus liquid bacterium suspension, is applicable in
In the bacillus liquid bacterium suspension of organic agriculture, wherein general bacillus liquid bacterium suspension has following weight percent composition
Composition：
Agriculture breast 34：2.68%~4.18%
D-425：2.32%~2.56%
OP-10：1.78%~2.06%
Potassium sorbate：0.48%~0.65%
Glycerine：4.96%~6.18%
Xanthans：0.16%~0.22%
Surplus group is divided into fermentation of bacillus liquid：
Bacillus liquid bacterium suspension suitable for organic agriculture
Organosilicon：2.83%~3.62%
Potassium sorbate：0.48%~0.65%
Sucrose：3.54%~4.26%
Xanthans：0.16%~0.22%
Surplus group is divided into fermentation of bacillus liquid；
The fermentation of bacillus liquid refers to the liquid bacteria suspension of TB1340, TB1344, TB1358.
2. the preparation method of the bacterium of liquid containing bacillus suspension described in claim 1, it is characterised in that carried out by following step：
It is prepared by general bacillus liquid bacterium suspension：
（1）Prepare Biocontrol Bacillus zymotic fluid；
（2）Each ingredient is mixed according to following ratio：
Agriculture breast 34：2.68%~4.18%
D-425：2.32%~2.56%
OP-10：1.78%~2.06%
Potassium sorbate：0.48%~0.65%
Glycerine：4.96%~6.18%
Xanthans：0.16%~0.22%
Remaining group is divided into fermentation of bacillus liquid；
（3）By mixed liquor turbula shaker, with the rotating speed of 200 rmp/min, continuously concussion mixes well, and bacterium suspension can be observed
Precipitation is not generated, it is evenly dispersed, place room temperature preservation；
It is prepared by the bacillus liquid bacterium suspension suitable for organic agriculture：
（1）Prepare Biocontrol Bacillus zymotic fluid；
（2）Each ingredient is mixed according to following ratio：
Organosilicon：2.83%~3.62%
Potassium sorbate：0.48%~0.65%
Sucrose：3.54%~4.26%
Xanthans：0.16%~0.22%
Remaining group is divided into fermentation of bacillus liquid；
（3）By mixed liquor turbula shaker, with the rotating speed of 200 rmp/min, continuously concussion mixes well, and bacterium suspension can be observed
It does not precipitate, it is evenly dispersed, place room temperature preservation.
3. application of the liquid bacteria suspension containing bacillus described in claim 1 in terms of improving bacterium suspension stability.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110447659A (en) * | 2019-08-15 | 2019-11-15 | 江苏苏滨生物农化有限公司 | A kind of bacillus amyloliquefaciens suspending agent and preparation method thereof |
| CN111592992A (en) * | 2019-02-21 | 2020-08-28 | 中国科学院南京土壤研究所 | Preparation method and application of bacillus amyloliquefaciens microbial inoculum |
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| CN111592992A (en) * | 2019-02-21 | 2020-08-28 | 中国科学院南京土壤研究所 | Preparation method and application of bacillus amyloliquefaciens microbial inoculum |
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Effective date of registration: 20210219 Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Applicant after: Tianjin Academy of Agricultural Sciences Address before: 300384 Tianjin Plant Protection Research Institute, 17 km away from Jinjing Road, Xiqing District, Tianjin Applicant before: TIANJIN INSTITUTE OF PLANT PROTECTION |
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180921 |