CN108548867A - Restoring method is simulated in vitro tissue ultrasonic image feature extraction - Google Patents
Restoring method is simulated in vitro tissue ultrasonic image feature extraction Download PDFInfo
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- CN108548867A CN108548867A CN201810057558.1A CN201810057558A CN108548867A CN 108548867 A CN108548867 A CN 108548867A CN 201810057558 A CN201810057558 A CN 201810057558A CN 108548867 A CN108548867 A CN 108548867A
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- vitro tissue
- ultrasonic image
- image feature
- animal flesh
- animal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N29/00—Investigating or analysing materials by the use of ultrasonic, sonic or infrasonic waves; Visualisation of the interior of objects by transmitting ultrasonic or sonic waves through the object
- G01N29/04—Analysing solids
- G01N29/06—Visualisation of the interior, e.g. acoustic microscopy
- G01N29/0654—Imaging
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2291/00—Indexing codes associated with group G01N29/00
- G01N2291/02—Indexing codes associated with the analysed material
- G01N2291/024—Mixtures
- G01N2291/02475—Tissue characterisation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2291/00—Indexing codes associated with group G01N29/00
- G01N2291/02—Indexing codes associated with the analysed material
- G01N2291/024—Mixtures
- G01N2291/02483—Other human or animal parts, e.g. bones
Abstract
A kind of in vitro tissue ultrasonic image feature extraction simulation restoring method, A. choose one piece of animal flesh that can envelope in vitro tissue completely；The animal flesh distance butchers the time within 48 hours, and the lean meat for including at least 1cm thickness is coated with；B. the echo of the ultrasound examination animal flesh is moderate echo；C. crosscutting at a kerf at lean meat coating top surface 0.5cm 2cm, and a muscle valve in homogeneous thickness is started, in vitro tissue is completely placed in the notch, and in vitro tissue is made to be enveloped completely by muscle valve；D. supersonic sounding is carried out to the in vitro tissue being coated in it across animal flesh with 10M 16M high-frequency linear arrays ultrasonic probes, extracts ultrasonic image feature.Clinical Practice proves, the present invention is simple and practical, and the ultrasonic image characteristic image quality of extraction is high, similar to in-vivo image structure, it greatly helps doctor diagnosed in art, has filled up technological gap that is accurate, clearly extracting ultrasonic image feature in vitro tissue art.
Description
Technical field
The present invention relates to a kind of methods that the in vitro tissue to operation excision carries out supersonic sounding, specifically, of the invention
Be related to it is a kind of first by vitro tissue simulation be reduced into the close attached state of tissue, then ultrasonic wave is recycled to be surpassed
Method of the sound shadow as feature extraction.The present invention is especially suitable for being diagnosed in art, and it is the engineering of ultrasonic artificial intelligence diagnosis
Practise mark image.
Background technology
Supersonic sounding be soft tissue, internal organs lesions diagnosis important image check method, soft tissue resolution is high,
It is radiationless, it can be repeated, especially there is significant superiority to the diagnosing tumor of the organs such as mammary gland, thyroid gland, liver.
In the course of surgery, the in vitro tissue sample cut off in art is used for quickly detecting with clinical and basic research two
Aspect meaning.Clinically, it can be that lesion fast qualitative, incisxal edge are sentenced in art to be used for quickly detecting diagnosis in vitro tissue sample
Fixed provide greatly helps, and doctor is helped to determine whether complete resection pathological tissues.Such as detect breast-conserving surgery for breast cancer sample
Doctor can be helped to judge whether excision extension is enough, second operation caused by avoiding excision insufficient.If lymph node resection is performed the operation,
Doctor can be helped to judge that lymph node whether there is metastasis of cancer rapidly using in vitro tissue supersonic sounding, doctor is helped to decide whether
It needs to carry out frozen section pathologic finding.In terms of basic medical research, research can be made by carrying out ultrasound detection in vitro tissue
Person obtains clearly lesion structural images, can accurately obtain characteristics of image, be compareed with pathological section, give clinical note
It releases, learning sample is provided for the machine learning of artificial intelligence diagnosis, the level of conventional Ultrasound diagnosis can be improved in turn.
However, after pathological tissues are removed in vitro, since it loses the neighbouring relationship organized with human peripheral, acoustics is special
Sign significantly changes, and internal cyto-architectural judgement is huge with body surface ultrasonoscopy difference.In vitro tissue tumour cell is most
The 3D shapes such as spherical in shape or irregular shape, ultrasound directly detect sample and are only capable of obtaining tangent line bit image, are in direct contact a both sides
Sample is difficult to form good combination with ultrasonic probe, and picture quality is difficult to ensure.Clinician utilizes based on transabdominal ultrasonography
Diagnosis is difficult to instruct to diagnose in art again.
Invention content
In view of the foregoing, the object of the present invention is to provide a kind of in vitro tissue ultrasonic image feature extractions to simulate reduction side
Method, this method first by vitro tissue simulation be reduced into the close attached state of tissue, then surpassed using ultrasonic wave
Sound shadow is as feature extraction.
To achieve the above object, the present invention uses following technical scheme：A kind of in vitro tissue ultrasonic image feature extraction mould
Quasi- restoring method,
A. one piece of animal flesh that in vitro tissue can be enveloped completely is chosen, the animal flesh is that distance butchers the time and exists
Animal flesh in 48 hours, the animal flesh include the lean meat coating of at least 1cm thickness；
B. the echo of animal flesh described in ultrasound examination is moderate echo；
C. crosscutting at a kerf at the coated top surface 0.5cm-2cm of lean meat, and a piece of flesh in homogeneous thickness is started
In vitro tissue is completely placed in the notch by meat valve, and in vitro tissue is made to be enveloped completely by muscle valve；
D. it is wrapped up 10M-16M high-frequency linear array ultrasonic probe daubing coupling agents and with film, across animal muscle valve to packet
The in vitro tissue overlayed in it carries out supersonic sounding, extracts ultrasonic image feature.
Preferably, the animal flesh is thin pork.
Preferably, the contact surface of the animal muscle valve and ultrasonic probe is without skin layer.
Preferably, the animal flesh usage time is within 6 hours.
Preferably, the animal flesh is fresh animal meat.
Preferably, the animal flesh is frost animal flesh, the meat net film of the frost animal or gloves package it is tight after with
Hot water thaws.
Clinical Practice proves that the present invention is simple and practical, and the ultrasonic image characteristic image quality of extraction is high, with in-vivo image
Structure is similar, and doctor is greatly helped diagnosed in art, has filled up in vitro tissue art accurate, has clearly extracted ultrasonic image
The technological gap of feature.
Description of the drawings
Fig. 1 is by the in vitro tissue striograph under water for carrying out ultrasonic image feature extraction；
Fig. 2 is that in vitro tissue is immersed in the striograph that ultrasonic image feature extraction is carried out in couplant；
Fig. 3 is in vitro tissue ultrasonic image feature extraction simulation restoring method flow chart of the present invention；
Fig. 4 is the striograph that the present invention carries out in vitro tissue ultrasonic image feature extraction；
Fig. 5 is the striograph for the tissue ultrasonic image feature extracted before non-removal of lesions.
Specific implementation mode
The present invention is described in detail below in conjunction with the accompanying drawings, and the same reference numbers in the drawings refer to function is identical or phase
As element.The embodiment of attached drawing description is exemplary, it is intended to for explaining the present invention, and should not be understood as to the present invention's
Limitation.
Clinically, in vitro tissue is used for quickly detecting and can be carried for diseased region fast qualitative, incisxal edge judgement in art
For greatly helping, doctor is helped to determine whether completely to have cut off pathological tissues.However, when pathological tissues are removed in vitro
Afterwards, since it loses the neighbouring relationship organized with human peripheral, acoustic feature significantly changes, internal cyto-architectural judgement and people
Body transabdominal ultrasonography image difference is huge.3D shapes, the ultrasounds such as the most spherical in shape or irregular shapes of in vitro tissue tumour cell are straight
Connect detect sample be only capable of obtain tangent line bit image, be in direct contact a both sides sample and be difficult to form good combination with ultrasonic probe,
Picture quality is difficult to ensure.Clinician is difficult to instruct to diagnose in art again using the diagnostic result based on transabdominal ultrasonography.
Therefore in vitro tissue is immersed in clean distilled water by some scholars, then, using ultrasonic wave in vitro tissue into
Row ultrasonic image feature extraction.Present the edge of lesion due to huge acoustic characteristic contrast between in vitro tissue and water anti-
Normal blur effect.As shown in Figure 1, the imaged image edge of extraction is presented unusual strong echo, and internal structure show it is unclear,
And sample is placed in liquid and is difficult to fixed position, can not provide reliable diagnosis basis to diagnosis in art.
In vitro tissue is immersed in couplant by some researchers, then, ultrasound is carried out in vitro tissue using ultrasonic wave
Image feature extracts.It is set off as shown in Fig. 2, the imaged image of extraction lacks echo with surrounding, is shown as unusual strong echo.Coupling
Mixture is easy to be organized to pollute, and stray echoes are presented, influence picture quality.In addition, distilled water, couplant immersion method are required to grasp
Author's hand-held ultrasound probe is overhang in medium, it is desirable that and fabulous stability operates with ordinary ultrasonic inspection and there is very big difference,
It brings inconvenience to examiner.
In order to as much as possible by vitro tissue simulation be reduced into the closely coupled state of tissue, make in vitro tissue
Good combination is formed with ultrasonic probe, clear, comprehensively and accurately in vitro tissue is ultrasonic to be extracted using ultrasonic wave
Image feature, as shown in figure 3, the present invention provides following method：
A. first, choosing one piece can be by animal flesh 1 that in vitro tissue envelopes completely.
It is required that：It is more shorter better that the animal flesh distance butchers the time, no longer than 2 days, includes at least the lean meat of 1cm thickness
Coating.The animal flesh is fresh state, and non-freezing state, no water filling do not go bad.
B. the echo of the ultrasound examination animal flesh is moderate echo.
Because human body subcutaneous fat, muscle echo be moderate echo, the purpose of the detection be prevent choose animal flesh be
Frost, water filling or rotten animal flesh, the in vitro tissue ultrasonic image for influencing extraction are inaccurate.If it is in strong echo to detect the animal flesh
It may be freezing state, water filling or rotten then to prompt the meat, discard the animal flesh, choose again.
C. a muscle valve 3 in homogeneous thickness is started at a kerf 2 crosscutting at lean meat coating top surface 0.5cm-2cm,
In vitro tissue 4 is completely placed in the notch, and in vitro tissue 4 is made to be enveloped completely by muscle valve 3.
Preferably animal flesh belt leather is not lain against behaviour to selected animal flesh by belt leather on one side if the animal flesh belt leather
Make on platform table top, making it, belt leather is not placed in top surface on one side, and the muscle valve 3 of cladding in vitro tissue 4 do not have with 5 contact surface of ultrasonic probe
There is skin layer, the purpose is to so that ultrasonic wave is preferably detected the in vitro tissue being coated in animal flesh across animal flesh.
When crosscutting apart from lean meat coating top surface 0.5cm-2cm, animal flesh had better not be cut into two parts, retained on one side
It is connected, opens coating, in vitro tissue is put into notch, adjust required orientation, cover coating, gently massage.
D. it is wrapped up by 5 daubing coupling agent of 10M-16M high-frequency linear arrays ultrasonic probe and with clean film, across animal muscle
Valve 3 carries out supersonic sounding to the in vitro tissue 4 being coated in it, extracts ultrasonic image feature.
It can be used repeatedly for animal flesh selected by the present invention, but usage time should be in 6 hours.
Animal flesh selected by the present invention does not freeze thin pork to be fresh, certainly, other animal meat tissues also can be selected, but selected
Animal flesh is used to prepare muscle valve one side preferably without skin layer, does not have fat meat tissue, the texture of meat to want fine and smooth.If selected
Animal meat tissue is freezed, is thawed with hot water after being wrapped up tightly with clean film or gloves, is not directly put into hot water or micro-
Wave stove thaws.
The present invention coats in vitro tissue using fresh animal flesh, and simulation is reduced into imaginary tissue, according to clinic
Ultrasound inspection methods are detected.Since the animal flesh of cladding in vitro tissue is soft fresh thin pork, make in vitro tissue with
Fresh pork can be coupled perfectly；Again since fresh pork texture is fine and smooth, and there is no water filling, fine simulation reduction is in vitro
Tissue and the closely coupled state of tissue, when Probe Ultrasonic Searching detects in vitro tissue, in vitro tissue is formed with ultrasonic probe
The in vitro tissue ultrasonic image feature of good combination, extraction is clear, and picture quality is high, as shown in Figure 4.
The one side contacted with ultrasonic probe due to the animal flesh chosen of the present invention again without skin layer, and, ultrasonic human body sheet
When body lesion, ultrasonic probe is needed across skin Layer Detection lesion, and such as the part marked with asterisk in Fig. 5, the two is compared, this hair
The ultrasonic image image resolution ratio and clarity of bright extraction are better than the body foci's organization ultrasonic imaged image directly extracted, very
Clearly.
In vitro tissue ultrasonic image feature extracting method Clinical Practice provided by the invention proves, simple and practical, extraction
Ultrasonic image characteristic image quality it is high, it is similar to in-vivo image structure, greatly help doctor diagnosed in art, be also people
The study of work intelligence machine provides the study image that can annotate, filled up in vitro tissue art it is accurate, clearly extract ultrasonic image
The technological gap of feature.
Finally it should be noted that：Above-described embodiments are merely to illustrate the technical scheme, rather than to it
Limitation；Although the present invention is described in detail referring to the foregoing embodiments, it will be understood by those of ordinary skill in the art that：
It can still modify to the technical solution recorded in previous embodiment, or to which part or all technical features into
Row equivalent replacement；And these modifications or substitutions, it does not separate the essence of the corresponding technical solution various embodiments of the present invention technical side
The range of case.
Claims (6)
1. restoring method is simulated in a kind of in vitro tissue ultrasonic image feature extraction, it is characterised in that：
A. one piece of animal flesh that in vitro tissue can be enveloped completely is chosen, the animal flesh is that butcher the time small 48 for distance
When in animal flesh, the animal flesh include at least 1cm thickness lean meat coating；
B. the echo of animal flesh described in ultrasound examination is moderate echo；
C. crosscutting at a kerf at the coated top surface 0.5cm-2cm of lean meat, and a piece of muscle valve in homogeneous thickness is started,
In vitro tissue is completely placed in the notch, and in vitro tissue is made to be enveloped completely by muscle valve；
D. it is wrapped up 10M-16M high-frequency linear array ultrasonic probe daubing coupling agents and with film, across animal muscle valve to being coated on
In vitro tissue in it carries out supersonic sounding, extracts ultrasonic image feature.
2. restoring method is simulated in vitro tissue ultrasonic image feature extraction according to claim 1, it is characterised in that：It is described
Animal flesh is thin pork.
3. restoring method is simulated in vitro tissue ultrasonic image feature extraction according to claim 2, it is characterised in that：It is described
The contact surface of animal muscle valve and ultrasonic probe is without skin layer.
4. restoring method is simulated in vitro tissue ultrasonic image feature extraction according to claim 3, it is characterised in that：It is described
Animal flesh usage time is within 6 hours.
5. restoring method is simulated in vitro tissue ultrasonic image feature extraction according to claim 4, it is characterised in that：It is described
Animal flesh is fresh animal meat.
6. restoring method is simulated in vitro tissue ultrasonic image feature extraction according to claim 4, it is characterised in that：It is described
Animal flesh is frost animal flesh, and the meat net film of the frost animal or gloves are thawed after wrapping up tightly with hot water.
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Citations (7)
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| US5055051A (en) * | 1990-08-03 | 1991-10-08 | Dornier Medical Systems, Inc. | Semi-anthropomorphic biliary/renal training phantom for medical imaging and lithotripsy training |
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|---|---|---|---|---|
| US5055051A (en) * | 1990-08-03 | 1991-10-08 | Dornier Medical Systems, Inc. | Semi-anthropomorphic biliary/renal training phantom for medical imaging and lithotripsy training |
| CN2232610Y (en) * | 1995-03-09 | 1996-08-07 | 四川联合大学 | Human body chest irradiation emulation body model |
| GB2328775B (en) * | 1997-08-29 | 2001-10-31 | Sami Ahmed Moussa | Simulator |
| CN1937956A (en) * | 2004-05-06 | 2007-03-28 | 日本电信电话株式会社 | Component concentration measuring device and method of controlling component concentration measuring device |
| CN101011238A (en) * | 2007-02-02 | 2007-08-08 | 华南师范大学 | Image-forming method and device for detecting HIFU action effect |
| CN202795877U (en) * | 2012-08-10 | 2013-03-13 | 天津市天堰医教科技开发有限公司 | Abdominothoracic damage ultrasonic evaluation training model |
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Application publication date: 20180918 |