CN108548710A - A kind of prefabricated examination powder and its application method for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid - Google Patents
A kind of prefabricated examination powder and its application method for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid Download PDFInfo
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- CN108548710A CN108548710A CN201810337699.9A CN201810337699A CN108548710A CN 108548710 A CN108548710 A CN 108548710A CN 201810337699 A CN201810337699 A CN 201810337699A CN 108548710 A CN108548710 A CN 108548710A
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- boric acid
- powder
- edta
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- nucleic acid
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
Abstract
The invention belongs to biological analysis fields, provide a kind of prefabricated examination powder and its application method for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid.Its constituent and weight are as follows：Based on the electrophoretic buffer for preparing mono- times of (1 ×) concentration of 1L, every part of prefabricated examination powder contains the Na of the Tris alkali of 9.6 12g, the boric acid of 4.89 6.11g, 0.186 0.744g2EDTA·2H2O, the KH of 3.40 3.67g2PO4.In use, at room temperature, it is sufficiently mixed with 1L deionized waters and is dissolved each other to get to the electrophoretic buffer of 1 × concentration of 1L per a examination powder.The present invention eliminates the multinomial drug of weighing of conventional method, adjusts the step of solution ph when preparing electrophoretic buffer so that the process for preparation of buffer solution is completed in 5 10 minutes, improves nucleic acid electrophoresis conventional efficient.
Description
Technical field
The invention belongs to biological analysis field, provides in a kind of biochemistry or molecular biology experiment analysis, be used for
The prefabricated examination powder and its application method that nucleic acid electrophoresis buffer solution is quickly prepared.
Background technology
Electrophoresis detection has critical role, is Nucleic acid quality and concentration in the biochemistry and molecular biological analysis of nucleic acid
One of conventional method of detection is analysis DNA and RNA clip sizes, the important evidence of mass concentration and purity.Traditional nucleic acid
Electrophoresis mainly uses acetate salt buffer system TAE (Tris-Acetic acid-EDTA) or borate buffer systems TBE (Tris-
Boric acid-EDTA).Relative to TAE buffer solutions, tbe buffer liquid has better Nucleic acid stabilization and longer electrophoresis longevity
Life, however, being TAE or tbe buffer liquid, is required for experimenter independently to prepare, including weighs multinomial drug and adjust pH value
And etc., waste more manpower and time.It is badly in need of a kind of electrophoresis that is simple, quickly, accurately preparing fixed concentration and pH value of exploitation
Buffer PROCEDURE, to improve electrophoresis experiment whole efficiency.
The present invention is insufficient for the above experiment demand and method, develops and a kind of quickly preparing the pre- of nucleic acid electrophoresis buffer solution
System examination powder, which is the tbe buffer liquid of improvement, uses Tris alkali, boric acid, Na2EDTA and KH2PO4For principal component, by one
Certainty ratio is mixed with deionized water dissolve each other after, can quickly prepare nucleic acid electrophoresis buffer solution in 5-10 minutes, it is real to reduce electrophoresis
It tests time, improves electrophoresis experiment efficiency.
Invention content
In view of the problems of the existing technology, the present invention provides a kind of quickly preparing nucleic acid electrophoresis buffering based on boric acid
The prefabricated examination powder of liquid.The main component of the examination powder includes Tris alkali (trishydroxymethylaminomethane), boric acid (H3BO3)、Na2EDTA
(disodium ethylene diamine tetraacetate) and KH2PO4(potassium dihydrogen phosphate).It is tradition using the electrophoretic buffer obtained by the examination powder
TBE (Tris-Boric acid-EDTA) the i.e. improvement systems of borate buffer solution.
In order to achieve the above object, technical scheme is as follows：
A kind of prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid, constituent and weight are as follows：
Based on the electrophoretic buffer for preparing mono- times of (1 ×) concentration of 1L, every part of prefabricated examination powder contains Tris alkali, the 4.89- of 9.6-12g
The boric acid of 6.11g, the Na of 0.186-0.744g2EDTA·2H2O, the KH of 3.40-3.67g2PO4.Thus the running buffer prepared
Each constituent is a concentration of in liquid：Tris alkali 80-100mmol/L, boric acid 80-100mmol/L, Na2EDTA 0.5-2mmol/
L, KH2PO425-27mmol/L, pH stable is between 7.8-8.2.All materials are thoroughly mixed in examination powder, with loose powder or pressure
Sheet form is stored or is encapsulated in plastic containers or bag.The Na2EDTA·2H2O and KH2PO4It can be by other containing difference
The Na of the crystallization water2EDTA is replaced, but Na2EDTA and KH2PO4Mass concentration remain unchanged.
The electrophoretic buffer based on preparation mono- times of (1 ×) concentration of 1L, each constituent and weight of prefabricated examination powder
Preferably：The Na of Tris alkali containing 10.8g, the boric acid of 5.5g, 0.744g in every part of prefabricated examination powder2EDTA·2H2O、3.53g
KH2PO4；Thus each constituent is a concentration of in the electrophoretic buffer prepared：Tris alkali 90mmol/L, boric acid 90mmol/L,
Na2EDTA 1mmol/L, KH2PO426mmol/L, pH of cushioning fluid is between 8.0-8.1.
A kind of application method of the prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid is as follows：
At room temperature, it is sufficiently mixed and dissolves each other to get to the electrophoresis of 1 × concentration of 1L with 1L deionized waters per a examination powder
Buffer solution.This process is simple and quick without adjusting pH of cushioning fluid without weighing examination powder.
A kind of preparation method of the prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid is as follows：
The prefabricated examination powder can zoom in or out the preparation of scale, be tried in powder with 1L 1 × concentration electrophoretic buffers
On the basis of each component mass fraction, according to the product of Target buffer liquid product and concentration, each component quality in benchmark is calculated
Zoom in or out multiple, weigh each component and after evenly mixing, with loose powder or compression forms, store or be encapsulated in plastic containers
Or in bag.It is exemplified below：It is such as equipped with 1L 10 × concentration electrophoretic buffers examination powder, then each component quality in benchmark is amplified to 10
Times, it is such as equipped with 5L 0.5 × concentration electrophoretic buffers and tries powder, then each component quality in benchmark is amplified to 2.5 times.
The prefabricated examination powder can also be prepared integrally, according to the mass ratio of the component, weighed and uniformly mixed each
Component is stored in plastic containers or bag.When preparing electrophoretic buffer, according to multiplying for the concentration of buffer solution to be matched and volume
Product first calculates required prefabricated examination silty amount, then weighs prefabricated examination powder, finally fully mixed with the deionized water of buffer solution volume to be matched
It closes, electrophoretic buffer is made.
Compared with prior art, beneficial effects of the present invention are：
Prefabricated examination powder provided by the invention is the tbe buffer liquid of improvement, and the use process of the prefabricated examination powder provided is easy to be fast
Speed uses Tris alkali, boric acid, Na2EDTA and KH2PO4For principal component, mixed by a certain percentage with deionized water after dissolving each other,
It eliminates the multinomial drug of weighing of conventional method, adjust the step of solution ph so that the process for preparation of buffer solution is at 5-10 points
It is completed in clock, quickly prepares nucleic acid electrophoresis buffer solution, reduce electrophoresis experiment time, improve electrophoresis experiment efficiency.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1
Based on the nucleic acid electrophoresis buffer solution for preparing 1 × concentration of 1L, prefabricated examination powder is prepared, every part of examination powder contains 10.8g's
The Na of Tris alkali, the boric acid of 5.5g, 0.744g2EDTA·2H2O, the KH of 3.53g2PO4.A examination powder is taken to be filled with 1L deionized waters
After dividing mixing, electrophoretic buffer (Tris alkali 90mmol/L, boric acid 90mmol/L, Na of 1 × concentration are obtained2EDTA 2mmol/L,
KH2PO426mmol/L, pH value 8.02), electrophoresis is after 5 hours, positive pH value 8.0, cathode pH value 8.4, nucleic acid samples in electrophoresis tank
Stablize, ultraviolet imagery is good.
Embodiment 2
Examination powder formula in embodiment 1 is adjusted slightly, based on the electrophoretic buffer for preparing 1 × concentration of 1L, every part prefabricated
Examination powder contains the Na of the Tris alkali of 10.8g, the boric acid of 5.5g, 0.372g2EDTA·2H2O, the KH of 3.4g2PO4.Take a examination powder
After being sufficiently mixed with 1L deionized waters, obtain 1 × concentration electrophoretic buffer (Tris alkali 90mmol/L, boric acid 90mmol/L,
Na2EDTA 1mmol/L, KH2PO425mmol/L, pH value 8.03), electrophoresis is after 5 hours, positive pH value 8.1, cathode in electrophoresis tank
PH value 8.3, nucleic acid samples are stablized, and ultraviolet imagery is good.
Embodiments of the present invention above described embodiment only expresses, but therefore can not be interpreted as special to the present invention
The limitation of the range of profit, it is noted that for those skilled in the art, without departing from the inventive concept of the premise,
Various modifications and improvements can be made, these are all belonged to the scope of protection of the present invention.
Claims (4)
1. a kind of prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid, which is characterized in that the prefabricated examination
Each constituent and weight are as follows in powder：Based on the electrophoretic buffer for preparing mono- times of (1 ×) concentration of 1L, every part of prefabricated examination powder contains
The Na of the Tris alkali of 9.6-12g, the boric acid of 4.89-6.11g, 0.186-0.744g2EDTA·2H2O, 3.40-3.67g
KH2PO4；Thus each constituent is a concentration of in the electrophoretic buffer prepared：Tris alkali 80-100mmol/L, boric acid 80-
100mmol/L、Na2EDTA 0.5-2mmol/L, KH2PO425-27mmol/L, pH stable is between 7.8-8.2；It tries in powder
All materials are thoroughly mixed, and are stored with loose powder or compression forms.
2. a kind of prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid according to claim 1, special
Sign is that described based on the electrophoretic buffer for preparing mono- times of (1 ×) concentration of 1L, each constituent and weight of prefabricated examination powder are excellent
It is selected as：The Na of Tris alkali containing 10.8g, the boric acid of 5.5g, 0.744g in every part of prefabricated examination powder2EDTA·2H2O, 3.53g
KH2PO4；Thus each constituent is a concentration of in the electrophoretic buffer prepared：Tris alkali 90mmol/L, boric acid 90mmol/L,
Na2EDTA 1mmol/L, KH2PO426mmol/L, pH of cushioning fluid is between 8.0-8.1.
3. a kind of prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid according to claim 1 or 2,
It is characterized in that, the Na2EDTA·2H2O and KH2PO4It can be by other Na containing the different crystallizations water2EDTA is replaced, it is desirable that
Na2EDTA and KH2PO4Mass concentration remain unchanged.
4. claims 1 or 2 or a kind of 3 any prefabricated examination powder for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid
Application method, it is characterised in that following steps：At room temperature, the examination powder of every portion and 1L deionized waters are sufficiently mixed mutually
It is molten to get to the electrophoretic buffer of 1 × concentration of 1L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810337699.9A CN108548710A (en) | 2018-04-12 | 2018-04-12 | A kind of prefabricated examination powder and its application method for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid |
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| CN201810337699.9A CN108548710A (en) | 2018-04-12 | 2018-04-12 | A kind of prefabricated examination powder and its application method for quickly preparing nucleic acid electrophoresis buffer solution based on boric acid |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327537A (en) * | 1999-12-02 | 2001-12-19 | 海茂株式会社 | Polyacrylamide precast gels for electrophoresis, process for producing the same and electrophoresis method by using the gels |
| CN103476930A (en) * | 2011-04-22 | 2013-12-25 | 索尼公司 | Method for electrophoresing nucleic acids, method for concentrating and purifying nucleic acids, cartridge for nucleic acid electrophoresis, and method for producing cartridge for nucleic acid electrophoresis |
-
2018
- 2018-04-12 CN CN201810337699.9A patent/CN108548710A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1327537A (en) * | 1999-12-02 | 2001-12-19 | 海茂株式会社 | Polyacrylamide precast gels for electrophoresis, process for producing the same and electrophoresis method by using the gels |
| CN103476930A (en) * | 2011-04-22 | 2013-12-25 | 索尼公司 | Method for electrophoresing nucleic acids, method for concentrating and purifying nucleic acids, cartridge for nucleic acid electrophoresis, and method for producing cartridge for nucleic acid electrophoresis |
Non-Patent Citations (1)
| Title |
|---|
| 刘桂元: "姜黄素诱导人髓母细胞瘤Daoy细胞自噬及其机制研究", 《中国优秀硕士学位论文全文数据库 医药卫生科技辑》 * |
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