CN108545804A - A method of bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique - Google Patents
A method of bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique Download PDFInfo
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- 241000894006 Bacteria Species 0.000 title claims abstract description 43
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 37
- 230000003647 oxidation Effects 0.000 title claims abstract description 36
- 239000006229 carbon black Substances 0.000 claims abstract description 27
- 238000002156 mixing Methods 0.000 claims abstract description 25
- 238000006243 chemical reaction Methods 0.000 claims abstract description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 6
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 5
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 5
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229910052753 mercury Inorganic materials 0.000 claims abstract description 4
- 239000007791 liquid phase Substances 0.000 claims abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 20
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 18
- 229910052799 carbon Inorganic materials 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000013019 agitation Methods 0.000 claims description 6
- 229910002804 graphite Inorganic materials 0.000 claims description 6
- 239000010439 graphite Substances 0.000 claims description 6
- 239000000693 micelle Substances 0.000 claims description 6
- 239000000126 substance Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 4
- 230000009977 dual effect Effects 0.000 claims description 3
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- 239000000203 mixture Substances 0.000 claims description 3
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 22
- 239000007788 liquid Substances 0.000 description 15
- 241000588724 Escherichia coli Species 0.000 description 10
- 241000191967 Staphylococcus aureus Species 0.000 description 10
- 239000007787 solid Substances 0.000 description 9
- 241000191963 Staphylococcus epidermidis Species 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 239000002131 composite material Substances 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
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- 239000002609 medium Substances 0.000 description 6
- 239000011734 sodium Substances 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 5
- 238000007747 plating Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 239000001888 Peptone Substances 0.000 description 3
- 108010080698 Peptones Proteins 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
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- 235000019319 peptone Nutrition 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- BFKJFAAPBSQJPD-UHFFFAOYSA-N tetrafluoroethene Chemical compound FC(F)=C(F)F BFKJFAAPBSQJPD-UHFFFAOYSA-N 0.000 description 3
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- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000186146 Brevibacterium Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 206010011409 Cross infection Diseases 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
- MSFSPUZXLOGKHJ-UHFFFAOYSA-N Muraminsaeure Natural products OC(=O)C(C)OC1C(N)C(O)OC(CO)C1O MSFSPUZXLOGKHJ-UHFFFAOYSA-N 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
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- 230000001580 bacterial effect Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
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- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
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- 235000013312 flour Nutrition 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
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- 239000001963 growth medium Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- 229910052708 sodium Inorganic materials 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/72—Treatment of water, waste water, or sewage by oxidation
- C02F1/722—Oxidation by peroxides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/30—Treatment of water, waste water, or sewage by irradiation
- C02F1/32—Treatment of water, waste water, or sewage by irradiation with ultraviolet light
-
- C—CHEMISTRY; METALLURGY
- C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
- C25B—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTION OF COMPOUNDS OR NON-METALS; APPARATUS THEREFOR
- C25B1/00—Electrolytic production of inorganic compounds or non-metals
- C25B1/01—Products
- C25B1/28—Per-compounds
- C25B1/30—Peroxides
-
- C—CHEMISTRY; METALLURGY
- C25—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES; APPARATUS THEREFOR
- C25B—ELECTROLYTIC OR ELECTROPHORETIC PROCESSES FOR THE PRODUCTION OF COMPOUNDS OR NON-METALS; APPARATUS THEREFOR
- C25B11/00—Electrodes; Manufacture thereof not otherwise provided for
- C25B11/04—Electrodes; Manufacture thereof not otherwise provided for characterised by the material
- C25B11/042—Electrodes formed of a single material
- C25B11/043—Carbon, e.g. diamond or graphene
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/02—Specific form of oxidant
- C02F2305/023—Reactive oxygen species, singlet oxygen, OH radical
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Electrochemistry (AREA)
- Materials Engineering (AREA)
- Metallurgy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Inorganic Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A method of bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique.This method includes two steps, and first step structure is used for H2O2The bioelectrochemical system of synthesis, the system use graphitic carbon black mixing mill piezoelectricity pole as cathode；The UV/H of second step structure sterilization2O2Advanced oxidation system, the H of the advanced oxidation system2O2Source is that electrode cathode is pressed in graphitic carbon black mixing mill, and the low pressure mercury lamp that power is 4W is as ultraviolet source, and reaction system carries out shading treatment, and the light path of ultraviolet source and advanced oxidation liquid-phase reaction system is 2cm, and the pH value of solution is adjusted to 7.0 ± 0.5.The present invention is for killing bacterium.
Description
Technical field
The present invention relates to a kind of methods for killing bacterium based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique.
Background technology
In the environment of human living, bacterium is ubiquitous.Especially in densely populated region, a large amount of pathogenic bacteria are latent
In air and aqueous medium.By taking air as an example, it is estimated that every cubic metre there are about 280,000 bacteriums, human lives and production are pacified
Huge hidden danger is caused entirely.Escherichia coli, staphylococcus aureus and staphylococcus albus are usually as thin in test air
The representative species of bacterium sum.Escherichia coli（Escherichia coli, E.coli）It is Gram-negative brevibacterium, size
0.5 × 1~3 microns.Peritrichous can move, no gemma；The various saccharides that can ferment production acid, aerogenesis, are in humans and animals enteron aisle
Normal perch bacterium, enter enteron aisle with lactation after baby due, all the life together with people, almost account for the 1/3 of excrement dry weight.Country
It provides, the total plate count in every milliliter of drinking water is less than 100, and total coli group must not be detected in every 100mL water.Golden yellow grape
Coccus (Staphylococcus aureus), also referred to as " S. aureus L-forms ", cell wall contain 90% peptide glycan and 10% teichoic acid.Its
The reticular structure of peptide glycan is not decolourized so presenting purple when dyeing than Gram-negative bacteria densification after crystal violet attachment by alcohol
Color, on the contrary, the whole cell peptidoglycan layer of negative bacterium is thin, the degree of cross linking is poor, lipid content is high, so purple compound is washed out by alcohol
Then it is attached to the red of husky of common dye.Staphylococcus aureus is a kind of important pathogen of the mankind, is under the jurisdiction of staphylococcus
（Staphylococcus）, have the nickname of " thermophilic meat bacterium ", be the representative of gram-positive bacteria, many severe infections can be caused.And
For amount of the staphylococcus aureus in quick-frozen food, the Ministry of Public Health announces food security country on November 24th, 2011
Standard《Quick-frozen wheat flour and rice products》, allow S. aureus L-forms to limit the quantity and exist.It is a group gram-positive cocci, because normal heap is polymerized to grape cluster
Shape, therefore gain the name, majority is non-pathogenic bacteria, and minority can lead to disease.Staphylococcus albus is most common pyococcus, is doctor
The important sources of institute's cross-infection, about 0.8 μm of thalline diameter, pellet shapes, but in the children phase culture of liquid medium within, usually divide
It dissipates, bacterial cell individualism.
Invention content
The object of the present invention is to provide a kind of UV/H2O2Technology has technological process simple, and oxidation efficiency is high, without secondary dirt
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique of dye.
Above-mentioned purpose is realized by following technical scheme：
A method of bacterium being killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique, this method includes two steps, first step structure
It builds and is used for H2O2The bioelectrochemical system of synthesis, the system use graphite-carbon black mixing mill piezoelectricity pole as cathode；Second step
Build the UV/H of sterilization2O2Advanced oxidation system, the H of the advanced oxidation system2O2Source is graphite-carbon black mixing mill piezoelectricity pole
Cathode, for the low pressure mercury lamp that power is 4W as ultraviolet source, reaction system carries out shading treatment, ultraviolet source and advanced oxidation liquid
The light path of phase reaction system is 2cm, and the pH value of solution is adjusted to 7.0 ± 0.5.
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique, the first step
Cathode preparation method, be by powdery graphite, powdered conductive carbon black by 5:1 ratio mixing, mixes with absolute ethyl alcohol,
10 min are stirred by ultrasonic, keep mixing carbon dust fully dispersed and are dissolved in absolute ethyl alcohol, under conditions of ultrasonic agitation, add dropwise
Enter ptfe emulsion, then 10 min are stirred by ultrasonic；Said mixture matter stirs 120min under 80 DEG C of water bath conditions, adds
Enter absolute ethyl alcohol and becomes micelle shape, continuous roll-in on roll squeezer by it, until compacting flakiness, is made graphite-carbon black
Mixing mill piezoelectricity pole.
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique, obtained by the first step
Graphite-carbon black mixing mill piezoelectricity pole be used for H2O2Synthesising biological electro-chemical systems；Graphite-carbon black mixing mill piezoelectricity pole is loaded
In the cathode chamber for entering dual chamber BES, anode chamber is placed into using carbon brush as anode；Anode is inoculated with waste water；50 are added in the cathodic compartment
The Na of mM concentration2SO4Solution is simultaneously constantly aerated；Construct graphite-carbon black mixing mill pressure electrode cathode bioelectrochemistry system
System synthesizes H in the cathode chamber of the system2O2。
Advantageous effect：
1. the present invention is based on the principle of UV/H2O2 advanced oxidations, in conjunction with the skill of bioelectrochemical system fabricated in situ hydrogen peroxide
Art, develop it is of low cost, it is simple for process, it is environmental-friendly, low energy consumption and to the formaldehyde in solution have high germicidal efficiency side
Method.
The present invention uses UV/H2O2 high-level oxidation technologies, excites dissociation H2O2 to generate Strong oxdiative hydrogen hydroxyl free using UV
Base（·OH）, and make the organic molecule of bacterium surface that oxidation reaction mineralising occur, to make cell rupture and death.
The present invention low energy consumption micro-current low-carbon environment-friendly, and the bacterium to being filled into solution has the excellent of high germicidal efficiency
Point is bioelectrochemistry advanced oxidation, efficient and non-secondary pollution.
Description of the drawings：
Attached drawing 1 is the compound roll-in cathode preparation flow figure of graphite-carbon black of the present invention.
Attached drawing 2 is bioelectrochemistry/advanced oxidation Fourier Series expansion technique method for disinfection flow chart of the present invention.
Attached drawing 3 is the effect contrast figure of the embodiment of the present invention 4.
Attached drawing 4 is the effect contrast figure of the embodiment of the present invention 5.
Attached drawing 5 is the effect contrast figure of the embodiment of the present invention 6.
Specific implementation mode：
Below in conjunction with the attached drawing of the present invention, technical scheme in the embodiment of the invention is clearly and completely described.
Embodiment 1：
A method of bacterium being killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique, this method includes two steps, first step structure
It builds and is used for H2O2The bioelectrochemical system of synthesis, the system use graphite-carbon black mixing mill piezoelectricity pole as cathode；Second step
Build the UV/H of sterilization2O2Advanced oxidation system, the H of the advanced oxidation system2O2Source is graphite-carbon black mixing mill piezoelectricity pole
Cathode, for the low pressure mercury lamp that power is 4W as ultraviolet source, reaction system carries out shading treatment, ultraviolet source and advanced oxidation liquid
The light path of phase reaction system is 2cm, and the pH value of solution is adjusted to 7.0 ± 0.5.
Bioelectrochemistry/advanced oxidation involved by this method couples sterilization system by being used for H2O2The bioelectrochemical of synthesis
System and ultraviolet/H2O2Advanced oxidation system two parts form.
Embodiment 2：
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique described in embodiment 1, described first
The preparation method of the cathode of step is by powdery graphite（Model HTF0325,40 μm of grain size, purity> 99.9 %）, it is powdered
Conductive carbon black（Model Vulcan XC-72R, 30 nm of grain size）By 5:1 ratio mixing, mixes with absolute ethyl alcohol, is stirred by ultrasonic
10 min keep mixing carbon dust fully dispersed and are dissolved in absolute ethyl alcohol, under conditions of ultrasonic agitation, polytetrafluoro is added dropwise
Vac emulsion, then 10 min are stirred by ultrasonic；Said mixture matter stirs 120min under 80 DEG C of water bath conditions, and anhydrous second is added
Alcohol becomes micelle shape, continuous roll-in on roll squeezer by it, until compacting flakiness, is made graphite-carbon black mixing roll-in
Electrode.
Embodiment 3：
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique described in embodiment 2, by first step institute
The graphite-carbon black mixing mill piezoelectricity pole obtained is used for H2O2Synthesising biological electro-chemical systems；Graphite-carbon black mixing mill piezoelectricity pole is filled
It inserts in the cathode chamber of dual chamber BES, anode chamber is placed into using carbon brush as anode；Anode is inoculated with waste water；It is added in the cathodic compartment
The Na of 50 mM concentration2SO4Solution is simultaneously constantly aerated；Construct graphite-carbon black mixing mill pressure electrode cathode bioelectrochemistry
System synthesizes H in the cathode chamber of the system2O2。
Embodiment 4：
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique described in embodiment 1,
Steps are as follows：
1）1g conductive blacks are weighed, 5g graphite carbon dusts are mixed with absolute ethyl alcohol, under conditions of ultrasonic agitation, are added dropwise poly-
Tetrafluoroethene lotion, then 10 min are stirred by ultrasonic；Compounding substances are stirred into 120min under 80 DEG C of water bath conditions, are added anhydrous
Ethyl alcohol becomes micelle shape, continuous roll-in on roll squeezer by it, until compacting flakiness, is made graphite-carbon black composite roll
Piezoelectricity pole；
2）The electrode is fitted into the cathode pool of bioelectrochemical system, using carbon brush as anode, is discharged as anode and is connect using BES
Kind liquid, with the Na of 50 mM concentration2SO4 Solution is as electrolyte；H will be synthesized2O2BES cathode solution press 1:1 ratio point
It does not react with the dilution of staphylococcus aureus, pH value is neutrality.
）The nutrient broth NB Liquid Culture based components of staphylococcus aureus include：Peptone:5 g, beef extract: 30
G, sodium chloride:5 g, distilled water:1000mL, pH:7.0 ~ 7.2, solid medium adds 15000 g/LmL agar powders.
Freeze-dried powder strain recovery incubation step is as follows：1. according to illustrate open ampoul tube take 0.2 mL NB culture mediums molten
Solve freeze-dried powder bacterium；2. respectively taking 0.1 mL lysate spread plates under aseptic conditions；3. is in 37 DEG C, constant incubator culture
2880 min；Then the staphylococcus aureus single bacterium for choosing recovery falls within 5 mL NB fluid nutrient mediums, in 37 DEG C, 120 rpm
1440 min of shaking table culture；It takes out and activates complete staphylococcus aureus, with 1 under aseptic condition:100 secondary cultures, 37
DEG C, 120 rpm shaking table cultures, 1440 min, secondary culture to the 3rd generation；Third generation staphylococcus aureus is taken out, in sterile item
Under part 10 are diluted to 10 times of dilution methods-6, take 0.1 mL applying solid plating mediums of bacterium solution after dilution；By original bacteria liquid 1：1 adds
Enter above-mentioned H2O2Solution and in 30 min of ultraviolet lower irradiation；Bacterium solution 1mL applying solid plating mediums after handling, in 37 DEG C of perseverances
1440 min of incubator culture is counted.
The experimental results showed that：The graphite-carbon black composite roll piezoelectricity pole 1. prepared according to step is installed to bioelectrochemistry system
In the cathode pool of system, under 2. experiment parameter that step is arranged, 30min is reacted, staphylococcus aureus is a concentration of in inoculation liquid
2.4*109 CFU/mL, after processing such as without detection viable bacteria（Attached drawing 3）.
Embodiment 5：
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique described in embodiment 1,
Steps are as follows：
1. weighing 1g conductive blacks, 5g graphite carbon dusts are mixed with absolute ethyl alcohol, under conditions of ultrasonic agitation, are added dropwise poly-
Tetrafluoroethene lotion, then 10 min are stirred by ultrasonic；Compounding substances are stirred into 120min under 80 DEG C of water bath conditions, are added anhydrous
Ethyl alcohol becomes micelle shape, continuous roll-in on roll squeezer by it, until compacting flakiness, is made graphite-carbon black composite roll
Piezoelectricity pole；
2. the electrode is fitted into the cathode pool of bioelectrochemical system, using carbon brush as anode, it is discharged as anode and is connect using BES
Kind liquid, with the Na of 50 mM concentration2SO4Solution is as electrolyte；H will be synthesized2O2BES cathode solution press 1:1 ratio point
It does not react with the dilution of staphylococcus albus, pH is neutral.
3. the LB liquid medium ingredient of staphylococcus albus includes：Peptone:10 g, yeast extract:5 g, chlorine
Change sodium:10 g, distilled water:1000mL, pH:7.0 ~ 7.5, solid medium adds 10000 g/mL agar powders.
Freeze-dried powder strain recovery incubation step is as follows：According to illustrate open ampoul tube take 0.2 mL LB culture mediums dissolving freeze
Dry powder bacterium, respectively takes 0.1 mL lysate spread plates under aseptic condition, 37 DEG C, 2880 min of constant incubator culture；Picking is multiple
The staphylococcus albus single bacterium of Soviet Union falls within 5 mL LB liquid mediums in 37 DEG C, 120 rpm shaking table cultures, 1440 min；It takes out
Complete staphylococcus albus is activated, aseptically 1:100 secondary cultures, 37 DEG C, 120 rpm shaking table cultures 1440
min；Secondary culture is to the 3rd generation；It takes out 3 platinite staphylococcuses, 10 is diluted to 10 times of dilution methods under aseptic condition-6.；It takes dilute
Release 0.1 mL applying solid plating mediums of rear bacterium solution；By original bacteria liquid 1：1 is added above-mentioned H2O2Solution and in it is ultraviolet it is lower irradiation 30
min.Bacterium solution 1mL applying solid plating mediums after handling are counted in 37 DEG C of 1440 min of insulating box culture.
The experimental results showed that：The graphite-carbon black composite roll piezoelectricity pole 1. prepared according to step is installed to bioelectrochemistry system
In the cathode pool of system, 30min is reacted, a concentration of 1*10 of staphylococcus albus in inoculation liquid5 CFU/mL, without detection viable bacteria after processing
Such as（Attached drawing 4）.
Embodiment 6：
A kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique described in embodiment 1,
Steps are as follows：
1. weighing 1g conductive blacks, 5g graphite carbon dusts are mixed with absolute ethyl alcohol, under conditions of ultrasonic agitation, are added dropwise poly-
Tetrafluoroethene lotion, then 10 min are stirred by ultrasonic；Compounding substances are stirred into 120min under 80 DEG C of water bath conditions, are added anhydrous
Ethyl alcohol becomes micelle shape, continuous roll-in on roll squeezer by it, until compacting flakiness, is made graphite-carbon black composite roll
Piezoelectricity pole；
2. the electrode is fitted into the cathode pool of bioelectrochemical system, using carbon brush as anode, it is discharged as anode and is connect using BES
Kind liquid, with the Na of 50 mM concentration2SO4 Solution is as electrolyte；H will be synthesized2O2BES cathode solution press 1:1 ratio point
It does not react with the dilution of Escherichia coli, pH is neutral.
3. the culture solution of Escherichia coli is LB liquid medium, peptone:10 g, yeast extract:5 g, sodium chloride:
10 g, distilled water:1000mL, pH:7.0 ~ 7.5, solid medium adds 10000 g/mL agar powders；It takes out in -80 DEG C of guarantors
The Escherichia coli glycerol stock deposited, 1 under aseptic condition:50 are inoculated in 5 mL culture mediums, 37 DEG C, 120 rpm shaking table cultures
1120min；It takes out and activates complete Escherichia coli, 1 under aseptic condition:100 secondary cultures, 37 DEG C, 120 rpm shaking table cultures
600 min；It takes out 2 generation Escherichia coli, 10-3 is diluted to 10 times of dilution methods under aseptic condition, 0.1 mL of bacterium solution is applied after taking dilution
Cloth solid plate culture medium；By original bacteria liquid 1：1 is added above-mentioned H2O2Solution and in 30 min of ultraviolet lower irradiation；Bacterium solution after handling
1mL applying solid plating mediums are counted in 37 DEG C of 1440 min of insulating box culture.
The experimental results showed that：The graphite-carbon black composite roll piezoelectricity pole 1. prepared according to step is installed to bioelectrochemistry system
In the cathode pool of system, 30min is reacted, e. coli concentration is 1.2*105 CFU/mL in inoculation liquid, only has 1 detection after processing
Viable bacteria（1CFU/mL, attached drawing 5）.
Claims (3)
1. a kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique, it is characterized in that:This method includes two
Step, first step structure are used for H2O2The bioelectrochemical system of synthesis, the system use graphite-carbon black mixing mill piezoelectricity pole conduct
Cathode；The UV/H of second step structure sterilization2O2Advanced oxidation system, the H of the advanced oxidation system2O2Source is mixed for graphite-carbon black
Close roll-in electrode cathode, power be 4W low pressure mercury lamp be used as ultraviolet source, reaction system carry out shading treatment, ultraviolet source and
The light path of advanced oxidation liquid-phase reaction system is 2cm, and the pH value of solution is adjusted to 7.0 ± 0.5.
2. a kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique according to claim 1,
It is characterized in that:The preparation method of the cathode of the first step is by powdery graphite, powdered conductive carbon black by 5:1 ratio
Example mixing, mixes with absolute ethyl alcohol, and 10 min are stirred by ultrasonic, and keeps mixing carbon dust fully dispersed and is dissolved in absolute ethyl alcohol,
Under conditions of ultrasonic agitation, ptfe emulsion is added dropwise, then 10 min are stirred by ultrasonic；Said mixture matter is at 80 DEG C
120min is stirred under water bath condition, and absolute ethyl alcohol is added and becomes micelle shape, continuous roll-in on roll squeezer by it, until pressure
It laminates, graphite-carbon black mixing mill piezoelectricity pole is made.
3. a kind of method that bacterium is killed based on bioelectrochemistry, advanced oxidation Fourier Series expansion technique according to claim 2,
It is characterized in that:Graphite-carbon black mixing mill piezoelectricity pole obtained by the first step is used for H2O2Synthesising biological electro-chemical systems；By graphite-
Carbon black mixing mill piezoelectricity pole is packed into the cathode chamber of dual chamber BES, and anode chamber is placed into using carbon brush as anode；Anode inoculation is useless
Water；The Na of 50 mM concentration is added in the cathodic compartment2SO4Solution is simultaneously constantly aerated；Construct graphite-carbon black mixing roll-in
Electrode cathode bioelectrochemical system synthesizes H in the cathode chamber of the system2O2。
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| CN109621701A (en) * | 2018-11-08 | 2019-04-16 | 天津大学 | A kind of device of biology dystopy degradation of organic substances |
| CN113292185A (en) * | 2021-04-25 | 2021-08-24 | 南京中微纳米功能材料研究院有限公司 | Multifunctional green pre-oxidation sewage treatment equipment and application method |
| CN113669834A (en) * | 2021-06-18 | 2021-11-19 | 天津大学 | Based on H2O2In situ synthesized UV/H2O2Indoor air sterilizing technology |
| CN114269691A (en) * | 2019-08-02 | 2022-04-01 | 懿华水处理技术有限责任公司 | Regulation of in-situ electrochemical generation of hydrogen peroxide for ultraviolet advanced oxidation process control |
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| CN113669834A (en) * | 2021-06-18 | 2021-11-19 | 天津大学 | Based on H2O2In situ synthesized UV/H2O2Indoor air sterilizing technology |
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Application publication date: 20180918 |