CN108542894A - A method of preparing charge reversal type nano particle using instantaneous nanometer sedimentation - Google Patents
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Abstract
The present invention relates to a kind of methods preparing charge reversal type nano particle using instantaneous nanometer sedimentation, include the following steps：Block copolymer A with negative electrical charge is dissolved in deionized water, as No. 1 liquid stream；Hydrophobic drug or fluorescent dye and protonated polymer B are dissolved in tetrahydrofuran, as No. 2 liquid streams；No. 3 liquid streams and No. 4 liquid streams are deionized water；It described No. 1, No. 2, No. 3 and No. 4 four bursts of liquid streams is injected to four-way simultaneously quickly liquidates and quickly mixed in vortex mixer, collect the nano suspending liquid formed, nano particle is prepared after dialysis removes organic solvent in the size that nano particle is adjusted by the velocity ratio adjusted between four bursts of liquid streams.
Description
Technical field
The present invention relates to a kind of charge reversal type nano particles of pH responses, are prepared specifically by instantaneous nanometer sedimentation
Charge reversal nano particle, belongs to new material technology field.
Background technology
In recent years, nano particle has become biology, material, doctor as the research of anti-biotic material or antineoplastic drug carrier
Worker's focus of attention.Nano material antibacterial or a key for carrying medicine treatment are how in different environments such as
PH stablizes nano material under ion concentration.And the distribution of nano-material surface charge greatly affects the stabilization of nanoparticle
Property, to determine the effective antibacterial or loading functional of nano particle.
The surface nature of nano particle also contributes to the final antibacterial or carrier medicine carrying efficiency of drug-loading nanoparticles.For example, nanometer
The surface charge of particle is just or excessively negative, and nano particle can be quickly by reticuloendothelial system in body fluid circulatory
(reticuloendothelial system, RES) is removed.Research shows that the surface charge of nano particle is neutral or slightly negative
(<- 15mV) there is macrocyclic property.However, when drug-loading nanoparticles are enriched to bacterium or tumor section, due to cell
Film surface current potential is negative, therefore surface charge is that positive nano particle can be by electrostatic interaction promotion bacterium or tumour cell to receiving
The phagocytosis of rice grain, it is just that this needs nanoparticle surface charge again.This contradictory demand is just promoting people anti-to charge
The research of transition nano particle.Research shows that the microenvironment of tumour cell is relatively low compared to the pH of normal blood of human body environment,
About 6.5~6.8.Using this characteristic, this characteristic can be realized by designing a series of pH response nanos material, to aobvious
Write the anti-cancer effectiveness for improving drug-loading nanoparticles.
Method prepared by current nano particle is broadly divided into emulsion-solvent evaporation method, spray drying, dialysis etc..However
These methods for preparing nano particle have its limitation.
Invention content
The present invention uses instantaneous nanoprecipitation method (Flash Nanoprecipitation, FNP), and it is anti-quickly to prepare charge
Make the transition nano particle, and preparation time only has several seconds, while can regulate and control the size of nano particle, the making by adjusting velocity ratio
Method has many advantages, such as that easy to operate, preparation time is extremely short, reproducible, easy industrial amplification, has great commercial Application
Foreground.
Specific technical solution is as follows：
A method of charge reversal type nano particle being prepared using instantaneous nanometer sedimentation, is included the following steps：
Block copolymer A with negative electrical charge is dissolved in deionized water, as No. 1 liquid stream；Hydrophobic drug or fluorescence dye
Material and protonated polymer B are dissolved in tetrahydrofuran, as No. 2 liquid streams；No. 3 liquid streams and No. 4 liquid streams are deionized water；
It described No. 1, No. 2, No. 3 and No. 4 four bursts of liquid streams is injected to four-way simultaneously quickly liquidates and quickly mixed in vortex mixer,
The nano suspending liquid formed is collected, the size of nano particle is adjusted by the velocity ratio adjusted between four bursts of liquid streams, is removed through dialysis
Nano particle is prepared after removing organic solvent.
Further, the block copolymer A with negative electrical charge be Dextran-b-PLGA based on glucan,
Dextran-b-PCL or Dextran-b-PLA.
Further, the block copolymer A：Dextran-b-PLGA configuration concentrations are 0.7-1.5mg/mL.
Further, the block copolymer A：Dextran-b-PLGA configuration concentrations are 1mg/mL, and pH is adjusted to 3.
Further, the block copolymer A with negative electrical charge is to be based on poly(ethylene glycol) methacrylate
(PEGMA) PEGMA-b-PCL, PEGMA-b-PLA or PEGMA-b-PLGA.
Further, the hydrophobic drug or fluorescent dye are beta carotene, anti-cancer medicine paclitaxel, camptothecine, four
Valence hydrophobicity cis-platinum or fluorescent dye ED (1-Ethyl-2- (4-dimethylaminostyryl) -4-
(dicyanomethylene)-1,4-dihydroquinoline)。
Further, the hydrophobic drug or fluorescent dye concentration range are respectively：Beta carotene solution concentration is
0.5-1mg/mL；Fluorescent dye ED solution concentrations are 0.02-0.05mg/mL.
Further, the beta carotene solution concentration is 0.5mg/mL；Fluorescent dye ED solution concentrations are 0.02mg/
mL。
Further, the protonated polymer B be PDMAEMA-b-PCL, PDMAEMA-b-PLGA,
PDMAEMA-b-PLA, Chitosan-g-PCL, Chitosan-g-PLA or Chitosan-g-PLGA.
Further, the block copolymer B：PDMAEMA-b-PCL configuration concentrations are 0.2-0.8 mg/mL.
Further, PDMAEMA-b-PCL configuration concentrations are 0.5mg/mL.
Further, the velocity ratio adjusted between four bursts of liquid streams is by adjusting No. 1 liquid stream and No. 2 liquid streams and No. 3
Velocity ratio between liquid stream and No. 4 liquid streams.The injection flow velocity of No. 1 liquid stream is 12mL/min；The injected current of No. 2 liquid streams
Speed is 12mL/min；The injection flow velocity of No. 3 liquid streams is 24~96 mL/min；The injection flow velocity of No. 4 liquid streams be 24~
96mL/min。
Further, 90 degree of the four plumes liquid interval, while injecting vortex centers.
The innovation of the invention consists in that a kind of novel nanometer grain preparation method is used, and by being simply blended two
Kind block copolymer realizes the effect of charge reversal.The preparation method is simple and efficient and easily controllable, in the diagnoses and treatment of tumour
Field has broad application prospects.
Description of the drawings
Fig. 1 is to prepare charge reversal nano particle schematic diagram；
Fig. 2 is that materials chemistry structural formula used is tested in the present invention；
Fig. 3 is the particle diameter distribution (DLS) that embodiment 1 prepares the charge reversal type nano particle for containing beta carotene；
Fig. 4 is the table for the charge reversal type nano particle that beta carotene is contained under ratio different in flow rate prepared by embodiment 1-4
Surface charge is with pH trend charts；
Fig. 5 is the grain that beta carotene charge reversal type nano particle is contained under ratio different in flow rate prepared by Examples 1 to 4
Diameter is with pH trend charts.
Fig. 6 is the charge reversal type nano particle that fluorescent dye ED is contained under ratio different in flow rate prepared by embodiment 5-8
Surface charge is with pH trend charts；
Fig. 7 is the charge reversal type nano particle that fluorescent dye ED is contained under ratio different in flow rate prepared by embodiment 5-8
Grain size is with pH trend charts；
Fig. 8 be embodiment 2 prepare contain beta carotene charge reversal type nano particle transmission electron microscope picture；
Fig. 9 be embodiment 2 prepare contain fluorescent dye ED charge reversal type nano particle transmission electron microscope pictures；
Figure 10 is the stability study for containing beta carotene charge reversal type nano particle prepared by embodiment 6.
Specific implementation mode
In the following, content is further illustrated the present invention with embodiment, but protection scope of the present invention is not limited in implementing
Example.The others that those skilled in the art makes without departing substantially from spirit of that invention and protection domain are changed and repaiied
Change, is included within the scope of the present invention.
Embodiment 1
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Beta carotene is dissolved in tetrahydrofuran, and concentration is all 0.5mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream 4 are deionized water, pH
It is 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4 respectively, and the flow velocity of liquid stream 1 and liquid stream 2 is set
It is set to 12mL/min, the flow velocity of liquid stream 3 and liquid stream 4 is set as 24mL/min, and four bursts of liquid streams enter four-way vortex mixing simultaneously
It is quickly mixed in device, collects the nano granule suspension of preparation, dialysis removes organic solvent, and as velocity ratio is 2： 1
The charge reversal type nano particle of (ratio of 3 flow velocity of liquid stream and 1 flow velocity of liquid stream).Product is characterized, and attached drawing 2 is DLS grain sizes
Analysis.
Embodiment 2
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Beta carotene is dissolved in tetrahydrofuran, and concentration is all 0.5mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream 4 are deionized water, pH
It is 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4 respectively, and the flow velocity of liquid stream 1 and liquid stream 2 is set
It is set to 12mL/min, the flow velocity of liquid stream 3 and liquid stream 4 is set as 48mL/min, and four bursts of liquid streams enter four-way vortex mixing simultaneously
It is quickly mixed in device, collects the nano granule suspension of preparation, dialysis removes organic solvent, and as velocity ratio is 4:1
Charge reversal type nano particle.
Embodiment 3
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Beta carotene is dissolved in tetrahydrofuran, and concentration is all 0.5mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream 4 are deionized water, pH
It is 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4 respectively, and the flow velocity of liquid stream 1 and liquid stream 2 is set
It is set to 12mL/min, the flow velocity of liquid stream 3 and liquid stream 4 is set as 72mL/min, and four bursts of liquid streams enter four-way vortex mixing simultaneously
It is quickly mixed in device, collects the nano granule suspension of preparation, dialysis removes organic solvent, and as velocity ratio is 6:1
Charge reversal type nano particle.
Embodiment 4
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；
PDMAEMA-b-PCL and beta carotene are dissolved in tetrahydrofuran, and concentration is all 0.5mg/mL, as liquid stream 2；Liquid
Stream 3 and liquid stream 4 are deionized water, pH 3.Two syringe pumps control the stream of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4 respectively
The flow velocity of speed, liquid stream 1 and liquid stream 2 is set as 12mL/min, and the flow velocity of liquid stream 3 and liquid stream 4 is set as 96mL/min, four strands of liquid
It flows while quickly being mixed into four-way vortex mixer is interior, collect the nano granule suspension of preparation, dialysis, which removes, to be had
Solvent, as velocity ratio are 8:1 charge reversal type nano particle.
Embodiment 5
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Fluorescent dye ED is dissolved in tetrahydrofuran, and concentration is respectively 0.5mg/mL and 0.02mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream
4 be deionized water, pH 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4,1 He of liquid stream respectively
The flow velocity of liquid stream 2 is set as 12mL/min, and the flow velocity of liquid stream 3 and liquid stream 4 is set as 24mL/min, and four bursts of liquid streams enter simultaneously
It is quickly mixed in four-way vortex mixer, collects the nano granule suspension of preparation, dialysis removes organic solvent, as
Velocity ratio is 2：The charge reversal type nano particle of 1 (ratio of 3 flow velocity of liquid stream and 1 flow velocity of liquid stream).Product is characterized, attached drawing
2 be DLS granularmetric analyses, and attached drawing 4 is Zeta Potential with pH value of solution mutation analysis, and attached drawing 3 is transmission electron microscope analysis.
Embodiment 6
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Fluorescent dye ED is dissolved in tetrahydrofuran, and concentration is respectively 0.5mg/mL and 0.02mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream
4 be deionized water, pH 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4,1 He of liquid stream respectively
The flow velocity of liquid stream 2 is set as 12mL/min, and the flow velocity of liquid stream 3 and liquid stream 4 is set as 48mL/min, and four bursts of liquid streams enter simultaneously
It is quickly mixed in four-way vortex mixer, collects the nano granule suspension of preparation, dialysis removes organic solvent, as
Velocity ratio is 4:1 charge reversal type nano particle.
Embodiment 7
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；PDMAEMA-b-PCL and
Fluorescent dye ED is dissolved in tetrahydrofuran, and concentration is respectively 0.5mg/mL and 0.02mg/mL, as liquid stream 2；Liquid stream 3 and liquid stream 4
For deionized water, pH 3.Two syringe pumps control the flow velocity of liquid stream 1 and liquid stream 2 and liquid stream 3 and liquid stream 4, liquid stream 1 and liquid respectively
The flow velocity of stream 2 is set as 12mL/min, and the flow velocity of liquid stream 3 and liquid stream 4 is set as 72mL/min, and four bursts of liquid streams enter four simultaneously
It is quickly mixed in the vortex mixer of channel, collects the nano granule suspension of preparation, dialysis removes organic solvent, as flows
Speed ratio is 6:1 charge reversal type nano particle.
Embodiment 8
Dextran-b-PLGA is dissolved in deionized water, a concentration of 1mg/mL, as liquid stream 1；
PDMAEMA-b-PCL and fluorescent dye ED are dissolved in tetrahydrofuran, and concentration is respectively 0.5mg/mL and 0.02mg/
ML, as liquid stream 2；Liquid stream 3 and liquid stream 4 are deionized water, pH 3.Two syringe pumps control liquid stream 1 and liquid stream 2 and liquid respectively
The flow velocity of stream 3 and liquid stream 4, the flow velocity of liquid stream 1 and liquid stream 2 are set as 12mL/min, and the flow velocity of liquid stream 3 and liquid stream 4 is set as
96mL/min, four bursts of liquid streams are quickly mixed into four-way vortex mixer is interior simultaneously, and the nano particle for collecting preparation is outstanding
Supernatant liquid, dialysis remove organic solvent, and as velocity ratio is 8:1 charge reversal type nano particle.
A kind of method quickly continuously preparing charge reversal type nano particle of the present invention, using instantaneous nanoprecipitation method,
In homemade multichannel swirl mixer, the block copolymer with pH responses quickly mixes to be formed with functional molecular
Nano particle with charge reversal type.This nano particle is negative in pH 7.4 (blood of human body pH value) surface charge, can be prolonged
The circulation time of long nano particle in vivo, when 6.6~6.8 pH, the surface charge reversion of nano particle becomes positive charge.
This method have many advantages, such as quickly, can industry amplify, answered with wide in nano-antibacterial, oncotherapy imaging diagnosis etc.
With value.
Claims (14)
1. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation, which is characterized in that including as follows
Step：
Block copolymer A with negative electrical charge is dissolved in deionized water, as No. 1 liquid stream；Hydrophobic drug or fluorescent dye and
Protonated polymer B is dissolved in tetrahydrofuran, as No. 2 liquid streams；No. 3 liquid streams and No. 4 liquid streams are deionized water；It will be described
No. 1, No. 2, No. 3 and No. 4 four bursts of liquid stream inject four-way and quickly liquidate simultaneously quickly to be mixed in vortex mixer, and shape is collected
At nano suspending liquid, by adjust four bursts of liquid streams between velocity ratio adjust nano particle size, through dialysis remove it is organic
Nano particle is prepared after solvent.
2. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, the block copolymer A with negative electrical charge is Dextran-b-PLGA, Dextran-b- based on glucan
PCL or Dextran-b-PLA.
3. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 2,
It is characterized in that, the block copolymer A：Dextran-b-PLGA configuration concentrations are 0.7-1.5mg/mL.
4. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 3,
It is characterized in that, the block copolymer A：Dextran-b-PLGA configuration concentrations are 1mg/mL.
5. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, the block copolymer A with negative electrical charge is based on poly(ethylene glycol) methacrylate (PEGMA)
PEGMA-b-PCL, PEGMA-b-PLA or PEGMA-b-PLGA.
6. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, the hydrophobic drug or fluorescent dye are that beta carotene, anti-cancer medicine paclitaxel, camptothecine, tetravalence are hydrophobic
Property cis-platinum or fluorescent dye ED.
7. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 6,
It is characterized in that, the hydrophobic drug or fluorescent dye concentration range are respectively：Beta carotene solution concentration is 0.5-1mg/
mL；Fluorescent dye ED solution concentrations are 0.02-0.05mg/mL.
8. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 7,
It is characterized in that, the beta carotene solution concentration is 0.5mg/mL；Fluorescent dye ED solution concentrations are 0.02mg/mL.
9. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, the protonated polymer B be PDMAEMA-b-PCL, PDMAEMA-b-PLGA, PDMAEMA-b-PLA,
Chitosan-g-PCL, Chitosan-g-PLA or Chitosan-g-PLGA.
10. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 9,
It is characterized in that, the block copolymer B：PDMAEMA-b-PCL configuration concentrations are 0.2-0.8mg/mL.
11. a kind of side preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 10
Method, which is characterized in that PDMAEMA-b-PCL configuration concentrations are 0.5mg/mL.
12. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, the velocity ratio adjusted between four bursts of liquid streams is by adjusting No. 1 liquid stream and No. 2 liquid streams and No. 3 liquid streams and 4
Velocity ratio between number liquid stream.
13. a kind of side preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 12
Method, which is characterized in that the injection flow velocity of No. 1 liquid stream is 12mL/min；The injection flow velocity of No. 2 liquid streams is 12mL/
min；The injection flow velocity of No. 3 liquid streams is 24~96mL/min；The injection flow velocity of No. 4 liquid streams is 24~96mL/min.
14. a kind of method preparing charge reversal type nano particle using instantaneous nanometer sedimentation according to claim 1,
It is characterized in that, 90 degree of the four plumes liquid interval, while injecting vortex centers.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110496251A (en) * | 2019-09-03 | 2019-11-26 | 上海微创医疗器械（集团）有限公司 | Cation nanometer drug and preparation method thereof carries medicine implanted medical device |
| CN111517333A (en) * | 2020-05-11 | 2020-08-11 | 石河子大学 | Porous SiO of three-dimensional hierarchical structure2Method for preparing microspheres |
| CN111545087A (en) * | 2020-04-27 | 2020-08-18 | 中山大学 | Multi-channel shunting high-efficiency homogeneous mixing device and application thereof in preparation of drug-loaded nanoparticles |
| CN114209658A (en) * | 2021-12-08 | 2022-03-22 | 华东理工大学 | Preparation method of GSH (glutathione) response type paclitaxel fluorescent nanoparticles |
| CN114350346A (en) * | 2021-12-09 | 2022-04-15 | 华东理工大学 | Method for preparing stable ICG dye-sensitized up-conversion nanoparticles |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669639A (en) * | 2017-09-30 | 2018-02-09 | 李岩 | A kind of method for preparing charge reversal type nano particle using instantaneous nanometer sedimentation |
-
2018
- 2018-02-14 CN CN201810151289.5A patent/CN108542894A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669639A (en) * | 2017-09-30 | 2018-02-09 | 李岩 | A kind of method for preparing charge reversal type nano particle using instantaneous nanometer sedimentation |
Non-Patent Citations (2)
| Title |
|---|
| MINGWEI WANG等: "Facile Preparation of AIE-Active Fluorescent Nanoparticles through Flash Nanoprecipitation", 《IND. ENG. CHEM. RES.》 * |
| 王铭纬等: "一种快速可控制备纳米粒子的新方法——瞬时纳米沉淀法研究进展", 《石河子大学学报：自然科学版》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110496251A (en) * | 2019-09-03 | 2019-11-26 | 上海微创医疗器械（集团）有限公司 | Cation nanometer drug and preparation method thereof carries medicine implanted medical device |
| WO2021043145A1 (en) * | 2019-09-03 | 2021-03-11 | 上海微创医疗器械（集团）有限公司 | Cationic nanodrug, preparation method therefor, and drug-loaded implantable medical device |
| CN111545087A (en) * | 2020-04-27 | 2020-08-18 | 中山大学 | Multi-channel shunting high-efficiency homogeneous mixing device and application thereof in preparation of drug-loaded nanoparticles |
| CN111545087B (en) * | 2020-04-27 | 2024-04-26 | 中山大学 | Multichannel diversion efficient homogeneous mixing device and application thereof in preparation of drug-loaded nano-particles |
| CN111517333A (en) * | 2020-05-11 | 2020-08-11 | 石河子大学 | Porous SiO of three-dimensional hierarchical structure2Method for preparing microspheres |
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