CN108541476A - A method of chemical inducer enhancing tomato is applied by external source and resists multiple disease - Google Patents
A method of chemical inducer enhancing tomato is applied by external source and resists multiple disease Download PDFInfo
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- CN108541476A CN108541476A CN201810222628.4A CN201810222628A CN108541476A CN 108541476 A CN108541476 A CN 108541476A CN 201810222628 A CN201810222628 A CN 201810222628A CN 108541476 A CN108541476 A CN 108541476A
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- tomato
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
It is specially a kind of to apply the method that chemical inducer enhancing tomato resists multiple disease by external source the invention belongs to vegetables and fruits pest control technical field.The method of the present invention is by chemical inducer allyl isothiazole（PBZ）It is blended in and pours in liquid filling, be applied to the root of 57 days tomato seedlings after being colonized；A concentration of 0.3 0.5 mM for pouring PBZ in liquid filling.The number of application is controlled at 12 times.The present invention can significantly increase resistance of the tomato to multiple diseases, reduce the generation of tomato disease by applying allyl isothiazole outside.The method of the present invention is easy to operate, and cost is relatively low.
Description
Technical field
The invention belongs to vegetables and fruits pest control technical fields, and in particular to side of the enhancing tomato to multiple diseases resistance
Method.
Technical background
Tomato（Scientific nameSolanum lycopersicum, English name tomato）, it is that Solanaceae tomato genus is annual or for many years
Raw herbaceous plant, body is 0.6-2 meters high, and the raw cement glandular hairs of entirety have overpowering odor, stem easily to lodge, and leaf pinnate compound leaf or pinniform are deep
Split, inflorescence always obstructs 2-5 centimetres long, normal 3-7 flower, calyx spoke shape, corolla spoke shape, berry oblate spheroid shape or close spherical, meat and it is more
Juice, seed yellow, flowering fruit bearing stage summer and autumn.The fruits nutrition of tomato is abundant, has flavour；And it can eat raw, cook, process
Catsup, juice or whole fruit canning.Therefore tomato is widely cultivated in China the South and the North various regions.
In actual production, tomato planting is for a long time by diseases such as early blight, late blight, gray mold, leaf mold, blossom-end rots
Harm.Disease seriously constrains the raising of tomato yield and quality.Currently, especially each big city Efforts To Develop in all parts of the country
It is built as a new round " vegetable basket project " for outstanding feature using facility, technicalization.Wherein facility vegetables and fruits turn " dish into
The core engineering of basket engineering ", it not only solves the light problem of guarantor of urban vegetable, and provides for city dweller abundant
Multi items vegetables and fruits.Soil-borne disease, virus disease are serious, and low temperature and high relative humidity disease becomes the obstacle of tomato production, physiological disturbance day
Become savage, seriously affects tomato normally production and greenhouse tomato industry development.And the production environment and producer of greenhouse tomato
Formula is different from traditional crop field tomato, and multiple crop index is high, and growth period is long, is more susceptible to the harm of disease and leads to its chemical fertilizer and pesticide
Amount of application be apparently higher than crop field tomato.Since greenhouse tomato is grown under the environmental condition of relative closure, product be easy because
Air flowing is slow, cannot get the reasons such as rainwater shower causes pesticide high residue to pollute, and which greatly limits pushing away for greenhouse tomato
Wide and development.
Allyl isothiazole（3-allyloxy-1,2-Benzisothiazole-1,1-dioxide, PBZ）It is a kind of effective
Prevent rice blast（Magnaporthe grisea）Efficient disease-resistance derivant（Watanabe, 1977; Watanabe
et al, 1979）.In isolated experiment, PBZ there's almost no antimicrobial bioactivity.To the microorganism of no pathogenicity
With warm-blooded animal low toxicity, safety, belong to a kind of environment amenable biological pesticide (Yi et al, 2006).The medicament is to planting
The normal growth and development of object has no significant effect, and is not easy to cause the drug resistance of pathogen（Kessmann et al, 1994）.PBZ is not
But can cause it is a variety of with accumulation of the immune defense in relation to enzyme in rice body, such as peroxidase, polyphenol oxidase and phenylpropyl alcohol
Propylhomoserin lyases etc.（Iwata et al, 1980; Sekizawa et al, 1981）, and can induce includingPBZ1WithRPR1The expression of defensin gene inside, to improve disease resistance of the rice to rice blast（Midoh et al, 1996 &
1997）.Compared to rice, Yoshioka etc. uses arabidopsis the investigation of materials anti-disease mechanism of PBZ, research to findNahG（Water
Poplar acid hydroxylase）Transfer-gen plant andnpr1On mutant strain, PBZ and BIT not can inducePR-1The expression of gene, butetr1
Withcoi1-1It can induce in mutant strainPR-1The expression of gene infers that SA and NPR1 rather than ethylene and JA are PBZ induced resistances
It is necessary, therefore, it is considered that PBZ participates in defense reaction by having activated the signal pathway that the SA/NPR1 of the upstreams SA is mediated
（Yoshioka et al, 2001）.Nakashita etc. is in tobacco it has also been found that similar as a result, the conjunction that PBZ passes through induction SA
At the Defense response reaction in activation plant body（Nakashita et al, 2002）.Moreover, this working group early-stage study
It was found that other than the disease resistance that document has been reported, the experiment tentatively promoted on rice, corn material shows that PBZ can also be lured
Water guide rice, corn multiple resistance.Therefore, it is suitable and feasible to promote application of the chemical inducer on tomato.Explore profit
With chemical inducer enhance the ability that main vegetables and fruits actively cope with stress from outside, rather than passes through agrochemical high poison, high residue
The new method of chemical pesticide meets the trend of social and economic development, has a very important significance.
Bibliography：
[1] Watanabe T (1977). Studies on rice blast controlling agent of
benzisothiazole analogs .2. Effects of probenazole (oryzemate) on each stage
of rice blast fungus (pyricularia-oryzae-cavara) in its life-cycle. Journal
of Pesticide Science, 2(4):395-404.
[2] Watanabe T, Sekizawa Y, Shimura M et al (1979). Effects of
probenazole (Oryzemate) on rice plants with reference to controlling rice
blast. Journal of Pesticide Science, 4:53-59.
[3] Yi XH, Lu YT (2006). Residues and dynamics of probenazole in rice
field ecosystem. Chemosphere, 65(4):639-643.
[4] Kessmann H, Staub T, Hofmann C et al (1994). Induction of systemic
acquired disease resistance in plants by chemicals. Annu ReV Phytopathol, 32:
439-459.
[5] Iwata M, Suzuki Y, Watanabe T et al (1980). Effect of probenazole on
the activities of enzymes related to the resistant reaction i n rice plant.
Ann.Phytopathol.Soc.Japan, 46:297-306.
[6] Sekizawa Y, Watanabe T (1981). On the mode of action of probenazole
against rice blast. Journal of Pesticide Science, 6(2):247-255.
[7] Midoh N, Iwata M (1996). Cloning and characterization of a
probenazole-inducible gene for an intracellular pathogenesis-related protein
in rice. Plant and Cell Physiology, 37(1):9-18.
[8] Midoh N, Iwata M (1997). Expression of defense-related genes by
probenazole or 1,2-benzisothiazole-3(2h)-one 1,1-dioxide. Journal of
Pesticide Science, 22(1):45-47.
[9] Yoshioka K, Nakashita H, Klessig DF et al (2001). Probenazole induces
systemic acquired resistance in Arabidopsis with a novel type of action.
Plant Journal, 25(2):149-157.
[10] Nakashita H, Yoshioka K, Yasuda M et al (2002). Probenazole induces
systemic acquired resistance in tobacco through salicylic acid accumulation.
Physiol Mol Plant Pathol, 61:197-203。
Invention content
It is induced the object of the present invention is to provide a kind of chemical inducer allyl isothiazole and improves tomato to multiple diseases resistance
Method.
The method that induction provided by the present invention improves tomato disease resistance, is by chemical inducer allyl isothiazole（PBZ）
It is blended in and pours in liquid filling, be applied to the root of 5-7 days tomato seedlings after being colonized（I.e. applying mode fills for root）.
Described to pour in liquid filling in the present invention, a concentration of 0.3-0.5 mM of PBZ, preferred concentration is 0.5 mM.
In the present invention, it is described apply be mixed with PBZ pour liquid filling, need continuous several times to apply, general number control is in 1-2
Secondary, preferably number is 2 times.
In the present invention, when continuous several times apply, front and back general 5-7 days of the 2 midfeather time is advisable.
Experiment shows that the method for the present invention can greatly improve the disease resistance of tomato.
The method of the present invention can be applied and close various tomato planting environment, such as chamber planting, field planting etc..
Method of the present invention by applying allyl isothiazole outside can significantly increase resistance of the tomato to multiple diseases, reduction kind
The generation of eggplant disease.The method of the present invention is easy to operate, and cost is relatively low.
Description of the drawings
Disease-resistant sex expression of Fig. 1 tomato varieties 1 under PBZ processing.
Disease-resistant sex expression of Fig. 2 tomato varieties 1 under water process.
Disease-resistant sex expression of Fig. 3 tomato varieties 2 under PBZ processing.
Disease-resistant sex expression of Fig. 4 tomato varieties 2 under water process.
Specific implementation mode
Embodiment 1 improves tomato disease resistance using chemical inducer PBZ.
1.1 tomato planting
Matrix culture:
Matrix components：Vermiculite：Black earth：Perlite=9: 3 : 0.5
Nutrient solution prescription：
。
After matrix is impregnated with nutrient solution, tomato seeds are seeded in nursery cave（Long 4cm, width 4cm, depth 3.5cm）In, 16h L/
8h D illumination carries out nursery under the conditions of 22 DEG C.It is transplanted seedlings after 28 days to big basin（Diameter 30cm, depth 25cm）In, matrix is impregnated with nutrition
Liquid.Field planting 5-7 days（It is calculated 27-29 days from sowing）I.e. for processing.
1.2 PBZ inductions are handled
By 0.5 mM PBZ additions in watering pours water, the tomato variety of different disease resistances is handled.Continuous processing 2 times
（The midfeather time is one week）Afterwards, the survival rate that most tomato varieties perform better than is found（Referring to Fig. 1-Fig. 4）.
Claims (3)
1. a kind of applying the method that chemical inducer enhances tomato to Disease Resistance by external source, which is characterized in that lure chemistry
It leads agent allyl isothiazole PBZ and is blended in and pour in liquid filling, be applied to the root of 5-7 days tomato seedlings after field planting.
2. according to the method described in claim 1, it is characterized in that, described pour in liquid filling, a concentration of 0.3-0.5 mM of PBZ.
3. according to the method described in claim 1, it is characterized in that, described apply the number control for pouring liquid filling for being mixed with PBZ
At 1-2 times.
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| CN201810222628.4A CN108541476A (en) | 2018-03-19 | 2018-03-19 | A method of chemical inducer enhancing tomato is applied by external source and resists multiple disease |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537944A (en) * | 2003-10-23 | 2004-10-20 | 复旦大学 | Promoter induced by plant system acquired character resistance inducer and its application |
| CN1539973A (en) * | 2003-10-25 | 2004-10-27 | 复旦大学 | Promotor induced by resistance inducer acquired by paddy system and application |
| CN102630512A (en) * | 2012-04-11 | 2012-08-15 | 复旦大学 | Method for improving abiotic stress resistance of plants to disease sources, drought and high salinity |
-
2018
- 2018-03-19 CN CN201810222628.4A patent/CN108541476A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1537944A (en) * | 2003-10-23 | 2004-10-20 | 复旦大学 | Promoter induced by plant system acquired character resistance inducer and its application |
| CN1539973A (en) * | 2003-10-25 | 2004-10-27 | 复旦大学 | Promotor induced by resistance inducer acquired by paddy system and application |
| CN102630512A (en) * | 2012-04-11 | 2012-08-15 | 复旦大学 | Method for improving abiotic stress resistance of plants to disease sources, drought and high salinity |
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