CN108535477A - A kind of rapid detection method of echinococcosis intermediate host lesion internal organs - Google Patents
A kind of rapid detection method of echinococcosis intermediate host lesion internal organs Download PDFInfo
- Publication number
- CN108535477A CN108535477A CN201810318966.8A CN201810318966A CN108535477A CN 108535477 A CN108535477 A CN 108535477A CN 201810318966 A CN201810318966 A CN 201810318966A CN 108535477 A CN108535477 A CN 108535477A
- Authority
- CN
- China
- Prior art keywords
- echinococcosis
- lesion
- antibody
- internal organs
- intermediate host
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 206010014096 Echinococciasis Diseases 0.000 title claims abstract description 27
- 208000009366 Echinococcosis Diseases 0.000 title claims abstract description 27
- 238000001514 detection method Methods 0.000 title claims abstract description 15
- 230000003902 lesion Effects 0.000 title claims abstract description 15
- 210000001835 viscera Anatomy 0.000 title claims abstract description 13
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 13
- 239000000427 antigen Substances 0.000 claims abstract description 11
- 102000036639 antigens Human genes 0.000 claims abstract description 11
- 108091007433 antigens Proteins 0.000 claims abstract description 11
- 241001494479 Pecora Species 0.000 claims abstract description 10
- 241000283690 Bos taurus Species 0.000 claims abstract description 9
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 6
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 6
- 239000010931 gold Substances 0.000 claims description 12
- 229910052737 gold Inorganic materials 0.000 claims description 12
- 239000000084 colloidal system Substances 0.000 claims description 9
- 210000002726 cyst fluid Anatomy 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 239000000284 extract Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 238000002372 labelling Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 230000002265 prevention Effects 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 238000007689 inspection Methods 0.000 abstract description 3
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 9
- 208000014674 injury Diseases 0.000 description 4
- 238000002559 palpation Methods 0.000 description 4
- 230000008733 trauma Effects 0.000 description 4
- 230000005856 abnormality Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 201000003808 Cystic echinococcosis Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 244000144980 herd Species 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012353 t test Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
- G01N2333/43526—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms
- G01N2333/43539—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms from cestodes
- G01N2333/43543—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from worms from cestodes from Taenia
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention provides a kind of rapid detection methods of echinococcosis intermediate host lesion internal organs, i.e., resist by the way that recombinant protein antigen expression, preparation are specific more, prepare colloidal gold strip.Using colloidal gold strip, testing result can be conveniently and efficiently obtained, can be used for cattle and sheep echinococcosis disease condition inspection in echinococcosis prevention and control.
Description
Technical field
The present invention relates to prevention and control of diseases fields, and in particular to a kind of quick inspection of echinococcosis intermediate host lesion internal organs
Survey method.
Background technology
The most important intermediate host in China echinococcosis Endemic Area is the livestocks such as ox, sheep, and cattle and sheep Hydatid Disease Infection rate can be anti-
Local echinococcosis prevalence is reflected, is an important parameter for weighing local echinococcosis popularity, is echinococcosis prevention and control
An important indicator.
Domestic and international cattle and sheep echinococcosis detection means includes mainly dissect digital palpation for examination of trauma, PCR identification methods.The country generally uses at present
Live dissect touches, in the method progress cattle and sheep echinococcosis that laboratory carries out PCR identifications to suspected abnormality and two methods combine
Detection.Dissect digital palpation for examination of trauma is completely dependent on the experience of skilled addressee, and error is larger；PCR method needs to be transported to related real again
It tests room to be identified, there is the shortcomings of transport is inconvenient, two methods, which are combined, then has the shortcomings of waiting for the testing result time long.
In short, existing main method there is to technical staff require it is high, be not easy to that base uses, result is not accurate enough, feedback is tied
Fruit not in time the shortcomings of.
Invention content
In view of this, the present invention provides a kind of rapid detection method of echinococcosis intermediate host lesion internal organs, that is, pass through
Recombinant protein antigen expression prepares the mostly anti-of specificity, prepares colloidal gold strip.It, can be conveniently fast using colloidal gold strip
Testing result is obtained promptly, be can be used for cattle and sheep echinococcosis disease condition inspection in echinococcosis prevention and control, can be examined in time
It looks into as a result, solving the problems, such as that PCR identification methods must transport to laboratory and be detected；Suspected abnormality is checked according to antigen-antibody reaction
In cyst fluid as a result accurately solve the problems, such as in work on the spot that digital palpation for examination of trauma is not accurate enough and personnel's experience dependence；Using colloid
Gold test paper strip method, it is easy to operate, it is used convenient for base's profession weak area, it is easy to carry, solution experiment condition requirement height,
As a result the problem of feeding back not in time；Stability is good, can long-term preservation.
An aspect of of the present present invention provides a kind of colloid gold test paper of quick detection echinococcosis intermediate host lesion internal organs,
It is characterized in that, by colloid gold particle prepare, determine the most suitable labelled amount of antibody, gold labeling antibody prepare purifying, treatment fluid selection,
Determine that quantity for spray, assembling are prepared.
Further, the antibody is the antibody that specificity is prepared using antigen-antibody reaction principle.
Further, the antigen is cyst fluid to be extracted from existing echinococcosis lesion, and then extract specific antigen, according to
Its sequence carries out recombinant protein antigen expression.
Another aspect of the present invention provides a kind of rapid detection method of echinococcosis intermediate host lesion internal organs, special
Sign is, is completed by following steps：Appropriate echinococcosis lesion is separately taken, appropriate cyst fluid is taken, drops in the sample-adding pad of colloid gold test paper
On, Site Detection cattle and sheep internal organ show result.
The advantage of the invention is that can solve live digital palpation for examination of trauma accuracy is poor, test in laboratory not in time with require high ask
Topic.Easy to operate, inexperienced people slightly demonstrates and can be used, and is suitble to scene large-scale application.Sample can be detected in batch,
Again can single part of detection, as a result display is fast.Infection for flocks and herds situation is understood in time, and number is provided for echinococcosis prevention and control policy development and adjustment
According to data.Stable reagent, can long term storage；As a result accurate, it mustn't test in laboratory；Easy to operate, layman, which slightly does, to be shown
Model also can be used, and the area to lack qualified technical personnel is suitble to carry out work on the spot.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1：The preparation of colloidal gold strip
1, recombinant protein antigen is expressed.Cyst fluid is extracted from existing echinococcosis lesion, and then extracts specific antigen, root
Recombinant protein antigen expression is carried out according to its sequence.
2, the mostly anti-of specificity is prepared.The antibody of specificity is prepared using antigen-antibody reaction principle.
3, colloidal gold strip is prepared.It prepared by colloid gold particle, determine prepared by the most suitable labelled amount of antibody, gold labeling antibody
Purifying, determines quantity for spray, assembling at treatment fluid selection, prepares simple, fast test strips.
4, it detects.Appropriate echinococcosis lesion is separately taken, appropriate cyst fluid is taken, drops in sample-adding pad, Site Detection cattle and sheep internal organ；
As a result it shows.10min-15min is shown as a result, providing foundation for the detection of cattle and sheep internal organ and subsequent processing.
The above, patent preferred embodiment only of the present invention, but the protection domain of patent of the present invention is not limited to
This, any one skilled in the art is in the range disclosed in patent of the present invention, according to the skill of patent of the present invention
Art scheme and its inventive concept are subject to equivalent substitution or change, belong to the protection domain of patent of the present invention.
Claims (4)
1. a kind of colloid gold test paper of quick detection echinococcosis intermediate host lesion internal organs, which is characterized in that pass through colloidal gold
Grain prepares, determines that the most suitable labelled amount of antibody, gold labeling antibody prepare purifying, treatment fluid selection, determine that quantity for spray, assembling are prepared
's.
2. colloid gold test paper according to claim 1, which is characterized in that the antibody is to utilize antigen-antibody reaction principle
Prepare the antibody of specificity.
3. colloid gold test paper according to claim 2, which is characterized in that the antigen is from existing echinococcosis lesion
Cyst fluid is extracted, and then extracts specific antigen, recombinant protein antigen expression is carried out according to its sequence.
4. a kind of rapid detection method of echinococcosis intermediate host lesion internal organs, which is characterized in that completed by following steps：Separately
Appropriate echinococcosis lesion is taken, appropriate cyst fluid is taken, is dropped on the sample-adding pad of colloid gold test paper described in claim 1, Site Detection
Cattle and sheep internal organ show result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810318966.8A CN108535477A (en) | 2018-04-11 | 2018-04-11 | A kind of rapid detection method of echinococcosis intermediate host lesion internal organs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810318966.8A CN108535477A (en) | 2018-04-11 | 2018-04-11 | A kind of rapid detection method of echinococcosis intermediate host lesion internal organs |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108535477A true CN108535477A (en) | 2018-09-14 |
Family
ID=63480866
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810318966.8A Pending CN108535477A (en) | 2018-04-11 | 2018-04-11 | A kind of rapid detection method of echinococcosis intermediate host lesion internal organs |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108535477A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN106093378A (en) * | 2016-05-30 | 2016-11-09 | 吉林大学 | Detection dog echinococcus granulosus infection colloidal gold immune chromatography test and preparation method |
-
2018
- 2018-04-11 CN CN201810318966.8A patent/CN108535477A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101949926A (en) * | 2010-08-04 | 2011-01-19 | 浙江大学 | Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card |
| CN102608330A (en) * | 2012-03-12 | 2012-07-25 | 中国疾病预防控制中心寄生虫病预防控制所 | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof |
| CN106093378A (en) * | 2016-05-30 | 2016-11-09 | 吉林大学 | Detection dog echinococcus granulosus infection colloidal gold immune chromatography test and preparation method |
Non-Patent Citations (3)
| Title |
|---|
| 丁淑琴 等: "细粒棘球蚴热休克蛋白 70重组抗原的免疫学特性研究", 《宁夏医学院学报》 * |
| 佟雪琪 等: "包虫病诊断抗原分子Eg-00512的筛选、重组表达及抗原性鉴定", 《中国病原生物学杂志》 * |
| 秦小平 等: "免疫酶和胶体金技术在包虫病诊断中的应用", 《临床和实验医学杂志》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Tiwari et al. | Effects of seropositivity for bovine leukemia virus, bovine viral diarrhoea virus, Mycobacterium avium subspecies paratuberculosis, and Neospora caninum on culling in dairy cattle in four Canadian provinces | |
| CN104804082A (en) | Immunofluorescence detection test strip and preparation method thereof for rapid quantitative detection of porcine epidemic diarrhea viruses | |
| CN104650234A (en) | Anti-AKR1B10 protein monoclonal antibody and applications thereof | |
| CN107918011A (en) | The detection method and detection kit of fungi | |
| CN109414456A (en) | Fat and its medical application | |
| CN106771208A (en) | Brucella antibody test strip | |
| Barile et al. | Approaches to identify pregnancy failure in buffalo cows | |
| CN105911277A (en) | Animal epidemic disease antibody virus colloidal gold quantitative detection system and preparation method thereof | |
| CN108845143A (en) | A kind of PCT-CRP dual card and preparation method thereof | |
| Giammarco et al. | Evaluation of brix refractometry to estimate immunoglobulin G content in buffalo colostrum and neonatal calf serum | |
| Davoodi et al. | Incidence of iodine deficiency and congenital goitre in goats and kids of Darreh Garm region, Khorramabad, Iran | |
| CN107271665A (en) | A kind of test strips for detecting salbutamol and its application | |
| CN101382549A (en) | Mycobacterium tuberculosis rapid detecting system and method | |
| CN108535477A (en) | A kind of rapid detection method of echinococcosis intermediate host lesion internal organs | |
| CN106053783A (en) | Quick time-resolved fluorescence immunoassay kit for detection of T cells infected with tuberculosis and detection method of kit | |
| CN110320364A (en) | A kind of double antibodies sandwich gold-immunochromatographyreagent reagent for assay box, and the preparation method and application thereof | |
| ONUMA et al. | Usefulness of monoclonal antibodies for detection of enzootic bovine leukemia cells | |
| CN105424941A (en) | AKR1B10 protein and reagent kit for liver cirrhosis diagnosis | |
| CN109856397B (en) | Colloidal gold detection card for rapidly detecting rabbit plague virus and preparation method thereof | |
| CN102608330A (en) | Immunochromatographic strip for detecting alveolar echinococcosis and preparation method thereof | |
| Wu | Effect of radioimmunoassay on accuracy of thyroid hormone detection | |
| CN204287191U (en) | The two-in-one immune colloid gold Rapid detection test strip of a kind of rice leaf spot bacteria and xanthomonas oryzae pv. oryzicola | |
| CN204575661U (en) | Beta-stimulants nine test card | |
| Valdmann et al. | Individual and combined effects of diseases and cytological endometritis on reproductive performance and culling of dairy cows: preliminary results | |
| Lee et al. | The detection of bovine estrus by lactoferrin monoclonal antibody |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 207 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Applicant after: Institute of parasitic disease control and prevention, China Center for Disease Control and Prevention (National Center for tropical diseases) Address before: 207 Ruijin 2nd Road, Huangpu District, Shanghai 200025 Applicant before: INSTITUTE OF PARASITIC DISEASES, CHINESE CENTER FOR DISEASES CONTROL AND PREVENTION |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180914 |