CN108531170A - A kind of surface has the carbon quantum dot and its preparation method and application of amphoteric ion structure - Google Patents
A kind of surface has the carbon quantum dot and its preparation method and application of amphoteric ion structure Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The present invention provides a kind of preparation method and application of carbon quantum dot of the surface with amphoteric ion structure.The specific steps are：Lecithin is pulverized the last lecithin soln for being dispersed in water, being disperseed；Obtained lecithin soln is transferred in hydrothermal reaction kettle, hydrothermal reaction kettle is heated certain time, natural cooling is taken out, obtains the thick solution of brown；The thick solution centrifugation of obtained brown, filtering are removed into residue, obtain brown clear solution；Obtained brown clear solution is freeze-dried, carbon quantum dot is obtained.Carbon quantum dot in the prior art is solved in vivo in the case of different pH values, stability is relatively low, the technical issues of can not realizing long-term cycle in vivo, the preparation method of the present invention is simple and easy to do, at low cost, and the carbon quantum dot being prepared has stability good, particle diameter distribution is uniform, the advantages such as monodispersity is good can be applied to the nano-carrier of drug especially antitumor drug, realize therapeutic purposes.
Description
Technical field
The invention belongs to fluorescent carbon technical field of nano material, and in particular to a kind of surface has the carbon of amphoteric ion structure
Quantum dot and its preparation method and application.
Background technology
Amphoteric ion structural molecule is a kind of molecule containing amphoteric ion group or zwitterion group, this substance
Positive and negative charge is distributed on same monomer, and Surface-modification of Nanoparticles amphoteric ion can maintain nanoparticles stable and inhibition
Protein non-specific absorption is to realize the cycle of internal long-acting stabilization.Amphoteric ion circle with cellular membrane biomimetic structure
Face can form efficiently hydration layer by ion electrostatic interaction, and the stability and anti-immunity that not only can effectively enhance nano particle are removed
Ability enhances its " passive " targeting ability by improving circulation time in vivo, and divides when with environment-responsive or bioactivity
After son is compound, " active " target function of nano particle can be also effectively realized, therefore " bisexual ion purification " has developed into nanometer
The new strategy of particle surface modification.
The nano material that carbon nanomaterial is mainly made of carbon as one kind has excellent optical property, in energy
The application in the fields such as source, agricultural, biology has been achieved for huge success.In biological field, usually it is applied to bio-imaging
And bio-sensing, carry medicine treatment.The form of carbon nanomaterial is very abundant, including fullerene, carbon nanotube, Nano diamond,
The newcomer of graphene and family：Carbon quantum dot.Carbon quantum dot is that researcher is preparing single wall using arc discharge method
A kind of novel nano fluorescent material serendipitous when carbon nanotube generally refers to discrete, torispherical of the size less than 10nm
Carbon nano-particles.Nowadays, the method for preparing carbon quantum dot is more and more, and preparing carbon quantum dot cost constantly reduces, and step is more next
Simpler, quantum yield (QY) is improved, while mass producing exploitation and also becoming closer to realize.In general, it makes
Preparation Method is broadly divided into two major classes " from bottom to top " and " from top to bottom ".Carbon quantum dot has many excellent performances, such as good
Optical signal stability, resistance to photobleaching, toxicity it is low etc..The unique optical physics and chemical property that carbon quantum dot is showed make
It is obtained to be widely used in researching and developing new bio-imaging probe, high-performance nano sensor and multifunctional nanocomposites.With
Organic molecule or biomolecule to carbon quantum dot carry out further surface modification can obtain it is a series of with different function
Nano-complex based on carbon nanomaterial.With going deep into for research, the nanocomposite based on fluorescent carbon quantum dot
Towards integration imaging in nano material based on by carbon quantum dot, the direction progress of the multiple functions such as medicine and treatment is carried
And differentiation, it is final to realize multifunction and diagnosis and treatment integration.
Carbon quantum dot needs to contact with blood in organism and body fluid etc. for a long time when as nano-medicament carrier.In life
The extrace llular pH of normal structure is 7.3-7.5 in object；The extracellular microenvironment of tumor locus is faintly acid, and pH value is general
In 6.2-6.8；Acidity further increases in tumour cell, and pH is generally in 4.0-6.0.Carbon quantum dot in acid condition, H+
It can be protonated with-COOH and-the OH effect on carbon quantum dot surface, to the cycle reduced in organism that can occur to reunite
Time can not be unable to get fully release in the presence of drug in turn steadily in the long term and be difficult to realize good therapeutic effect.
Invention content
In the case of solution carbon quantum dot in the prior art in vivo different pH values, stability is relatively low, can not
The technical issues of realizing long-term cycle in vivo, the present invention provide a kind of carbon quantum dot of the surface with amphoteric ion structure
Preparation method and application.The preparation method of the present invention is simple and easy to do, at low cost, and the carbon quantum dot being prepared has stability
Good, the advantages such as particle diameter distribution is uniform, and monodispersity is good can be applied to the nano-carrier of drug especially antitumor drug,
Realize therapeutic purposes.
In order to solve the above technical problems, the present invention provides following technical solutions：
The first purpose of the invention is to provide a kind of surface have amphoteric ion structure carbon quantum dot preparation method,
It the described method comprises the following steps：
S1：Lecithin is pulverized the last lecithin soln for being dispersed in water, being disperseed；
S2：The lecithin soln that S1 is obtained is transferred in hydrothermal reaction kettle, hydrothermal reaction kettle is heated into certain time, from
It is so cooling, it takes out, obtains the thick solution of brown；
S3：The thick solution centrifugation of brown, the filtering that S2 is obtained remove residue, obtain brown clear solution；
S4：The brown clear solution freeze-drying that S3 is obtained, obtains carbon quantum dot.
Further, in S1, the dispersion ratio is to be dispersed in 20~100mL water per 1g lecithin powders, the water
For deionized water.
Further, in S2, the hydrothermal reaction kettle is polytetrafluoroethylene (PTFE) hydrothermal reaction kettle, and the heating temperature is 170
~200 DEG C, the heating time is 6~24 hours, and the natural cooling is to naturally cool to 10~35 DEG C.
Further, in S3, the centrifugation is 10~30min of centrifugation under conditions of 11000~14000rpm；The mistake
Filter is filtered for the filter membrane for the use of aperture being 220nm.
Further, in S4, the temperature of the freeze-drying is -60~-45 DEG C, the vacuum degree of freeze-drying is 8~
The processing time of 9Pa, freeze-drying are 24~48h.
Second object of the present invention is to provide the carbon that surface prepared by any one of aforementioned method has amphoteric ion structure
Quantum dot.
Further, it is 2.5~4.3nm, interplanar distance that the surface, which has the carbon quantum dot grain size of amphoteric ion structure,
For 0.3~0.4nm.
Third object of the present invention is to provide aforementioned surfaces to have the carbon quantum dot of amphoteric ion structure in Nano medication
Application in carrier.
Further, the carbon quantum dot that surface has amphoteric ion structure is taken to be dispersed in the solution containing chemotherapeutics,
Dispersion concentration is 1mg/mL, and chemotherapeutics, dialysis washing are adsorbed in immersion for 24 hours, and gained sample is divided into two groups by freeze-drying, point
It is not dispersed in the PBS of pH=5.0 and 7.4, then two groups of samples is respectively put into 1mL bag filters, bag filter is immersed in
In the PBS of 10mL same pH (i.e. the pH of PBS is identical inside and outside bag filter), different time points take the PBS outside 1mL bag filters, use
Ultra-violet analysis drug concentration draws release profiles, studies the release amount of medicine under different time points and different pH ranges.
Further, the chemotherapeutics is adriamycin (DOX).
It is had the beneficial effect that possessed by the present invention：
(1) it is carbon source that preparation method of the invention, which uses the lecithin (Phospholipid) with amphoteric ion structure,
Therefore the carbon quantum dot surface prepared has amphoteric ion structure, and biological safety is good, will not be made to environmental and biological materials
At damage.Meanwhile lecithin derives from a wealth of sources, simple and easy to get, cost is relatively low.
(2) any surface passivator need not be added in preparation method of the invention, and a step, which can prepare surface, to be had
The carbon quantum dot of amphoteric ion structure, enormously simplifies preparation process.
(3) surface prepared by the present invention has the carbon quantum dot of amphoteric ion structure, and stability is good, and particle diameter distribution is equal
Even, monodispersity is good, the drug release of pH responses can may be implemented with carrying medicament.
(4) the carbon quantum dot size uniformity prepared by the present invention, and there is good water solubility, cell compatibility is good,
Blood compatibility is good, can be applied to the nano-carrier of drug, realizes therapeutic purposes.
(5) carbon quantum dot prepared by the present invention realizes the performance with hair different colours light by adjusting excitation wavelength,
Under the light irradiation of different wave length, it can show the fluorescence of different colours, realize the fluorescence imaging of more colors.
Description of the drawings
Fig. 1 is the carbon quantum dot reaction process schematic diagram that surface prepared by the present invention has amphoteric ion structure.
Fig. 2 a are the carbon quantum dot transmission electron microscope pictures that surface prepared by the present invention has amphoteric ion structure；
B is the carbon quantum dot grain size distribution that surface prepared by invention has amphoteric ion structure.
Can clearly it find out, which shows as uniform spheric granules, is about with dispersibility, grain size well
2.9nm。
Fig. 3 is the uv absorption spectra that the present invention is the carbon quantum dot that prepared surface has amphoteric ion structure
(in illustration, 1 is the carbon quantum dot under white light conditions, and 2 be the carbon quantum dot under ultraviolet lamp).Show that carbon quantum dot exists in figure
There are one strong absorption peaks at 240nm, this is because caused by π-π * electron transitions.
Fig. 4 is that be prepared surface have the carbon quantum dot of amphoteric ion structure under different excitation wavelengths to the present invention
Fluorescence spectra.Show that carbon quantum dot possesses excitation wavelength dependence.
Fig. 5 is the XRD spectrum figure that the present invention is the carbon quantum dot that prepared surface has amphoteric ion structure, is shown in figure
Show that characteristic absorption peak occurs at 22.1 ° in carbon quantum dot, corresponding interplanar distance is 0.4nm, has good crystal structure.
Fig. 6 is the infrared spectrogram that the present invention is the carbon quantum dot that prepared surface has amphoteric ion structure,
3420cm-1There are the stretching vibration of O-H or N-H in place, this is carbon quantum dot surface, and there is the feature of hydroxyl and electropositive amino to inhale
Receive peak, 1626cm-1The absorption peak at place is the stretching vibration of P=O, 1070cm-1Locate absorption peak to the stretching vibration for P-O, shows
Phosphate groups are contained on carbon quantum dot surface, in 1720cm-1Absorption peak is the absorption peak of carboxyl, shows carbon quantum dot surface
Containing different electrical groups, further relating to carbon quantum dot has amphoteric ion structure.
Fig. 7 is the hemolysis rate that the present invention is the carbon quantum dot that prepared surface has amphoteric ion structure.Hemolysis rate is real
It tests and is often used as evaluation biomedical material compatibility index, the safety material Valuation Standard of biocompatibility：It is molten
Blood rate needs to be less than 5%, and prepared carbon quantum dot hemolysis rate under various concentration is respectively less than 5%, shows carbon quantum dot tool
There is good biocompatibility.
Fig. 8 is the Cytotoxic evaluation that the present invention is the carbon quantum dot that prepared surface has amphoteric ion structure, table
The growth that prepared carbon quantum dot does not interfere with cell is illustrated, there is good biocompatibility.
Fig. 9 is the present invention,
Release profiles under condition of different pH show carbon quantum dot in different acidity condition drug releasing rate difference, acid condition
Rate of release is very fast, and drug release is responded with pH.
Specific implementation mode
Embodiment 1
There is the carbon quantum dot of amphoteric ion structure to prepare with the following method on surface：
The first step：1g lecithin is dispersed in 30mL water.
Second step：It transfers the solution into reaction kettle, is reacted 8 hours under conditions of 180 DEG C.
Third walks：Until when reaction kettle is cooled to 15 DEG C, it is then centrifuged for 12000rpm 30min, then with 220nm filter membrane mistakes
It filters off and removes residue.
4th step：It is freeze-dried under the conditions of -60 DEG C, vacuum degree 8Pa, drying time is for 24 hours.
Prepared carbon quantum dot is characterized with transmission electron microscope, test result average grain diameter is 2.9nm.Pass through what is obtained
For product quality than the quality of upper reactant, gained yield is 53.12%.
Embodiment 2
There is the carbon quantum dot of amphoteric ion structure to prepare with the following method on surface：
The first step：1g lecithin is dispersed in 100mL water.
Second step：It transfers the solution into reaction kettle, is reacted 24 hours under conditions of 200 DEG C.
Third walks：Until when reaction kettle is cooled to 10 DEG C, it is then centrifuged for 11000rpm 20min, then with 220nm filter membrane mistakes
It filters off and removes residue.
4th step：It is freeze-dried under the conditions of -60 DEG C, vacuum degree 8Pa, drying time is for 24 hours.
Prepared carbon quantum dot is characterized with transmission electron microscope, test result average grain diameter is 4.3nm.Pass through what is obtained
For product quality than the quality of upper reactant, gained yield is 51.64%.
Embodiment 3
There is the carbon quantum dot of amphoteric ion structure to prepare with the following method on surface：
The first step：1g lecithin is dispersed in 20mL water.
Second step：It transfers the solution into reaction kettle, is reacted 6 hours under conditions of 170 DEG C.
Third walks：Until when reaction kettle is cooled to 35 DEG C, it is then centrifuged for 14000rpm 10min, then with 220nm filter membrane mistakes
It filters off and removes residue.
4th step：It is freeze-dried under the conditions of -45 DEG C, vacuum degree 9Pa, drying time 48h.
Prepared carbon quantum dot is characterized with transmission electron microscope, test result average grain diameter is 3.3nm.Pass through what is obtained
For product quality than the quality of upper reactant, carbon quantum dot yield is 53.99%.
Embodiment 4
There is the carbon quantum dot of amphoteric ion structure to prepare with the following method on surface：
The first step：1g lecithin is dispersed in 100mL water.
Second step：It transfers the solution into reaction kettle, is reacted 24 hours under conditions of 190 DEG C.
Third walks：Until when reaction kettle is cooled to 25 DEG C, being then centrifuged for 13000rpm 25min.
4th step：It is freeze-dried under the conditions of -55 DEG C, vacuum degree 9Pa, drying time 48h, then with 220nm filter membrane mistakes
It filters off and removes residue.
Prepared carbon quantum dot is characterized with transmission electron microscope, test result average grain diameter is 3.1nm.Pass through what is obtained
For product quality than the quality of upper reactant, carbon quantum dot yield is 55.76%.
The solution that carbon quantum dot of the surface with amphoteric ion structure prepares 0.5mg/mL is made in Examples 1 to 4, use is glimmering
It is as follows that light protractor measures its fluorescence intensity results.
| Group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Fluorescence intensity | 812 | 786 | 701 | 755 |
Embodiment 5
Appropriate dilute concentration surface is taken to have the water-soluble drop of carbon quantum dot of amphoteric ion structure on copper mesh, after room temperature is dried
With transmission electron microscope (H-7650, Japan) observe sample morphology, see attached drawing 2 as can be seen that the nano-particles size it is uniform, dispersion
Uniformly, grain size is about 2.9nm.
Embodiment 6
The carbon quantum dot aqueous solution that appropriate dilute concentration surface has amphoteric ion structure is taken to utilize UV, visible light spectrophotometric
Its absorption of measurement examination, seeing attached drawing 3, there are one strong absorption peaks at 240nm.
Embodiment 7
The carbon quantum dot for having amphoteric ion structure with the surface of deionized water dispersion synthesis, is measured not with fluorescence protractor
With the fluorescent emission spectrogram under excitation wavelength.As shown in Fig. 4, the fluorescence intensity of carbon quantum dot is in the increase of excitation wavelength
Downward trend after first rising, and maximum excitation wavelength is 340nm, maximum emission wavelength 450nm.The transmitting of carbon quantum dot
Polynary excitation, the spectral characteristic of polynary transmitting is presented in wavelength gradual red shift with the increase of excitation wavelength.It thus is seen that sharp
The carbon quantum dot made from preparation method provided by the invention has excitation wavelength dependence, can be presented under different excitations
Go out different colors.
Embodiment 8
Appropriate carbon quantum dot is taken to be tested using D/MAX-rC types conversion target X-ray diffractometer, test condition：Cu targets, K α are penetrated
Line, tube voltage 40kV, tube current 100mA, 10 °/min of sweep speed show that carbon quantum dot occurs at 22.1 ° in attached drawing 5
Characteristic absorption peak, corresponding interplanar distance are 0.4nm, have good crystal structure.
Embodiment 9
It takes appropriate carbon quantum dot to carry out KBr tablettings, infrared absorption is tested using infrared spectrometer (Nexus 670, USA),
Instrument test condition：Scanning range 4000-400cm-1, resolution ratio：4cm-1, scanning times 64 times.As shown in fig. 6, in 3420cm-1There are the stretching vibration of O-H or N-H in place, this is the characteristic absorption peak that carbon quantum dot surface has hydroxyl and electropositive amino,
1626cm-1The absorption peak at place is the stretching vibration of P=O, 1070cm-1Locate absorption peak to the stretching vibration for P-O, shows carbon
Quantum dot surface contains phosphate groups, in 1720cm-1Absorption peak is the absorption peak of carboxyl, shows carbon quantum dot surface and contains
Different electrical groups, further relating to carbon quantum dot has amphoteric ion structure.
Embodiment 10
There is the carbon quantum dot of amphoteric ion structure to be each configured to 50 μ g/mL, 100 μ g/mL surface with physiological saline,
200 μ g/mL, the solution of 400 μ g/mL.10min is centrifuged to fresh rabbit blood at 1500 rpm, supernatant is removed, with PBS under
Layer red blood cell is washed, then with normal saline dilution, and the surface that various concentration is separately added into 10mL centrifuge tubes has two
Property ionic structure carbon quantum dot solution 4.8mL, add the 200 diluted red blood cells of μ L, 37 DEG C of item be placed on after rocking uniformly
Part hatches 3h, is centrifuged later to it, rotating speed 1500rpm, time 10min.Its supernatant is taken to carry out the test of absorbance, wavelength is set
545nm is set, OD is recorded ast；The diluted red blood cells of 200 μ L are added in 10mL deionized waters and are set as positive group, absorbance value note
Record is ODpc；The diluted red blood cells of 200 μ L are added in the physiological saline of 10mL as negative group, absorbance is recorded as ODnc.It is molten
The calculation formula of blood rate：Hemolysis rate %=[(ODt-ODnc)/(ODpc-ODnc)] × 100%.As shown in fig. 7, prepared carbon amounts
Son point hemolysis rate under various concentration is respectively less than 5%, and showing carbon quantum dot has good biocompatibility.
Embodiment 11
The cytotoxicity for using MTT colorimetric method test samples herein configures 50 μ g/mL, 100 μ g/ using DMEM culture solutions
The surface of mL, 200 μ g/mL, 400 μ g/mL have the carbon quantum dot solution of amphoteric ion structure, HeLa cells are taken out, in wine
Culture bottle is opened under smart lamp, original culture solution is abandoned, cell surface is cleaned using PBS buffer solutions, the pancreas egg of 1mL is added
White enzyme takes appropriate 10% fetal calf serum DMEM culture solutions that it is made to stop digestion after it is fully digested, and piping and druming makes cell detachment shape
At single cell suspension, 96 orifice plates are selected, the cell of 100 μ L is added per hole, are placed it in straight in 37 DEG C of carbon dioxide incubator
It is paved with to bottom hole by cell, removes culture solution original in hole, the surface for being separately added into various concentration has amphoteric ion structure
100 μ L of carbon quantum dot solution, each concentration be inoculated with 3~6 holes.Then 96 orifice plates are placed on to 37 DEG C of carbon dioxide incubator
Middle placement for 24 hours, is taken out later, and 20 μ L MTT solution are added into per hole, continues to be placed in 4h in incubator, liquid in hole is taken
Go out, 100 μ L of DMSO be added in every hole, gently shakes to crystal and fully dissolves, the O.D. values per hole are measured using microplate reader,
570nm is arranged in wavelength.Cell activity calculation formula：[(ODt-ODb)/(ODnc-ODb)] × 100%, ODt：Sample absorbance,
ODnc：Negative control group absorbance, ODbFor blank absorbency.As shown in figure 8, surface under the conditions of various concentration have both sexes from
The carbon quantum dot cell survival rate of minor structure is very high, without overt toxicity, does not interfere with the growth of cell, has good biology
Compatibility.
Embodiment 12
The carbon quantum that the surfaces 2mg have amphoteric ion structure is taken to be dispersed in 2mL DOX solution (DOX solution concentrations 2mg/
ML), impregnate and adsorb drug for 24 hours, then dialysis washing, gained sample is divided into two groups, is dispersed in pH=respectively by freeze-drying
In 5.0 and 7.4 PBS, then each sample is respectively put into 1mL bag filters, bag filter is immersed in 10mL pH=respectively
5.0 with (pH of PBS is identical inside and outside bag filter) in 7.4 PBS, (0,1,2,4,8,12,24,36,48,72 is small for different time points
When) PBS outside 1mL bag filters is taken to draw release profiles with ultra-violet analysis drug concentration.As shown in figure 9, carbon quantum dot is in acid
Property under the conditions of drug releasing rate it is very fast, alkaline condition release it is slower, with pH response drug release.
It is carbon source that the preparation method of the present invention, which uses the lecithin with amphoteric ion structure, is derived from a wealth of sources, simple and easy to get,
Cost is relatively low, and the carbon quantum dot surface prepared has amphoteric ion structure, and biological safety is good, will not be to environment and biology
Damage caused by body.Meanwhile any surface passivator need not be added, a step, which can prepare surface, has amphoteric ion knot
The carbon quantum dot of structure, simplifies preparation process.
The obtained surface of the present invention has the carbon quantum dot of amphoteric ion structure, and stability is good, and particle diameter distribution is uniform, single
The drug release of pH responses can may be implemented with carrying medicament in good dispersion；It can realize that there is hair not by adjusting excitation wavelength
It can show the fluorescence of different colours under the light irradiation of different wave length with the performance of color of light, realize the glimmering of more colors
Light is imaged.And size uniformity has good water solubility, and cell compatibility is good, and blood compatibility is good, can be applied to medicine
The nano-carrier of object realizes therapeutic purposes.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that those skilled in the art without
It needs creative work according to the present invention can conceive and makes many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of surface have amphoteric ion structure carbon quantum dot preparation method, which is characterized in that the method includes with
Lower step：
S1：Lecithin is pulverized the last lecithin soln for being dispersed in water, being disperseed；
S2：The lecithin soln that S1 is obtained is transferred in hydrothermal reaction kettle, hydrothermal reaction kettle is heated certain time, it is naturally cold
But, it takes out, obtains the thick solution of brown；
S3：The thick solution centrifugation of brown, the filtering that S2 is obtained remove residue, obtain brown clear solution；
S4：The brown clear solution freeze-drying that S3 is obtained, obtains carbon quantum dot.
2. preparation method according to claim 1, which is characterized in that in S1, the dispersion ratio is per 1g lecithin powders
It is dispersed in 20~100mL water, the water is deionized water.
3. preparation method according to claim 1, which is characterized in that in S2, the hydrothermal reaction kettle is polytetrafluoroethylene (PTFE) water
Thermal response kettle, the heating temperature are 170~200 DEG C, and the heating time is 6~24 hours, and the natural cooling is cooling
To 10~35 DEG C.
4. preparation method according to claim 1, which is characterized in that in S3, the centrifugation is 11000~14000rpm's
Under the conditions of centrifuge 10~30min；Described be filtered into is filtered using the filter membrane that aperture is 220nm.
5. preparation method according to claim 1, which is characterized in that in S4, the temperature of the freeze-drying is -60~-45
DEG C, the vacuum degree of freeze-drying is 8~9Pa, and the processing time of freeze-drying is 24~48h.
6. surface prepared by any one of claim 1 to 5 method has the carbon quantum dot of amphoteric ion structure.
7. surface according to claim 6 has the carbon quantum dot of amphoteric ion structure, which is characterized in that the surface tool
It is 2.5~4.3nm to have the carbon quantum dot grain size of amphoteric ion structure, and interplanar distance is 0.3~0.4nm.
8. the surface described in claim 6 or 7 has application of the carbon quantum dot of amphoteric ion structure in nano-medicament carrier.
9. application according to claim 8, which is characterized in that the carbon quantum dot that surface has amphoteric ion structure is taken to disperse
In the solution containing chemotherapeutics, chemotherapeutics, dialysis washing are adsorbed in dispersion concentration 1mg/mL, immersion for 24 hours, and freezing is done
It is dry, gained sample is divided into two groups, is dispersed in respectively in the PBS of pH=5.0 and 7.4, is then respectively put into 1mL bag filters,
Bag filter is immersed in the PBS of 10mL same pH, different time points take the PBS outside 1mL bag filters, with ultra-violet analysis drug
Concentration draws release profiles, studies the release amount of medicine under different time points and different pH ranges.
10. application according to claim 9, which is characterized in that the chemotherapeutics is adriamycin.
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| CN106629660A (en) * | 2016-12-22 | 2017-05-10 | 南京师范大学 | Preparation method of N, P co-doping carbon quantum dots, and product and application thereof |
| CN107115319A (en) * | 2017-04-05 | 2017-09-01 | 汪涛 | A kind of biological safety carbon quantum dot load adriamycin complex and its preparation method and application |
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| CN106629660A (en) * | 2016-12-22 | 2017-05-10 | 南京师范大学 | Preparation method of N, P co-doping carbon quantum dots, and product and application thereof |
| CN107115319A (en) * | 2017-04-05 | 2017-09-01 | 汪涛 | A kind of biological safety carbon quantum dot load adriamycin complex and its preparation method and application |
Non-Patent Citations (1)
| Title |
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| YIFANG YUAN ET AL.: "Doxorubicin-loaded environmentally friendly carbon dots as a novel drug delivery system for nucleus targeted cancer therapy", 《COLLOIDS AND SURFACES B: BIOINTERFACES》 * |
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