CN108529012A - A kind of autologous tissue stem cell applies constant temperature save set in vitro - Google Patents
A kind of autologous tissue stem cell applies constant temperature save set in vitro Download PDFInfo
- Publication number
- CN108529012A CN108529012A CN201810310657.6A CN201810310657A CN108529012A CN 108529012 A CN108529012 A CN 108529012A CN 201810310657 A CN201810310657 A CN 201810310657A CN 108529012 A CN108529012 A CN 108529012A
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- babinet
- fixed
- vitro
- constant temperature
- stem cell
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 28
- 238000000338 in vitro Methods 0.000 title claims abstract description 22
- 239000004065 semiconductor Substances 0.000 claims abstract description 19
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims description 3
- 230000006835 compression Effects 0.000 claims description 2
- 238000007906 compression Methods 0.000 claims description 2
- 238000005057 refrigeration Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 238000013016 damping Methods 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/18—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents providing specific environment for contents, e.g. temperature above or below ambient
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0273—Transport containers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D25/00—Details of other kinds or types of rigid or semi-rigid containers
- B65D25/02—Internal fittings
- B65D25/10—Devices to locate articles in containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D81/00—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents
- B65D81/02—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage
- B65D81/05—Containers, packaging elements, or packages, for contents presenting particular transport or storage problems, or adapted to be used for non-packaging purposes after removal of contents specially adapted to protect contents from mechanical damage maintaining contents at spaced relation from package walls, or from other contents
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Environmental Sciences (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of autologous tissue stem cells to apply constant temperature save set in vitro, including babinet, the rear side of the babinet upper end and the lower end of cover board are rotatablely connected by hinge, the underrun elastic device of box house is fixedly connected with the lower surface of gas-permeable nets, the side of gas-permeable nets and the inner wall of babinet are slidably connected, there are two placing boxes for the upper surface fixation of gas-permeable nets, the upper surface of two placing boxes is mounted on buckling device, elastic device improves the damping performance of placing box, rubber pneumatic bag prevents the collection bag equipped with mesenchymal stem cells from shaking, ensure the activity of mesenchymal stem cells；When the temperature in babinet is less than set temperature, PLC controller controls wind turbine and semiconductor chilling plate work, wind turbine makes the cold air that semiconductor chilling plate generates be uniformly distributed in babinet, the temperature of babinet maintains setting range, improves the reliability that this autologous tissue stem cell applies constant temperature save set in vitro.
Description
Technical field
The present invention relates to stem cell Techniques of preserving field, specially a kind of autologous tissue stem cell applies constant temperature to preserve in vitro
Device.
Background technology
Containing in placenta has a large amount of mescenchymal stem cell, and mescenchymal stem cell has multi-lineage potential, hematopoiesis support
It the features such as with stem cell implantation, immunoregulation and self-replacation is promoted, is concerned by people.Therefore, placenta is often located
Reason extraction mescenchymal stem cell, doctor carry out constant temperature preservation to mesenchymal stem cells.Mesenchymal stem cells mainly use collection bag at present
It holds, is equipped with protection liquid and preserves, be placed in heat-proof device, while low temperature environment is maintained with biological ice bag.Existing equipment structure is multiple
It is miscellaneous, and collection bag can collide and rub in transportational process, influence the activity of mescenchymal stem cell.
Invention content
The technical problem to be solved by the present invention is to overcome the existing defects, provides a kind of autologous tissue stem cell and applies in vitro
Constant temperature save set, simple in structure, elastic device improves the damping performance of placing box, and rubber pneumatic bag prevents dry thin equipped with mesenchymal
The collection bag of born of the same parents shakes, and ensures the activity of mesenchymal stem cells, can effectively solve the problems in background technology.
To achieve the above object, the present invention provides the following technical solutions：A kind of autologous tissue stem cell applies constant temperature in vitro
Save set, including babinet, the rear side of the babinet upper end and the lower end of cover board are rotatablely connected by hinge, the bottom of box house
Face is fixedly connected by elastic device with the lower surface of gas-permeable nets, and the side of gas-permeable nets and the inner wall of babinet are slidably connected, and is breathed freely
There are two placing boxes for the upper surface fixation of net, and the upper surface of two placing boxes is mounted on buckling device, the bottom surface of box house
It is fixed with PLC controller, temperature sensor and wind turbine, the position that babinet right side corresponds to wind turbine offers mounting hole, mounting hole
In be fixed with semiconductor chilling plate, the refrigeration end of semiconductor chilling plate is towards the inside of babinet, the input terminal of PLC controller and outer
The output end of portion's power supply is electrically connected, the output end input with the input terminal of semiconductor chilling plate and wind turbine respectively of PLC controller
End electrical connection, the output end electrical connection of the input terminal and temperature sensor of PLC controller.
As a preferred technical solution of the present invention, the leading flank of the cover board is fixed with ultraviolet radiator, ultraviolet radiator
Input terminal and PLC controller output end electrical connection.
As a preferred technical solution of the present invention, the bottom surface of the box house is fixed with air pump, the outlet of air pump
Mouth is connected to the air inlet of babinet by conduit, and the air inlet of air pump is connected to the air inlet of check valve by conduit, and babinet is right
The second gas nozzle, the input terminal of air pump and the output end electrical connection of PLC controller are fixed in the mounting hole of side.
As a preferred technical solution of the present invention, the buckling device includes transverse slat, and the lower surface of transverse slat is fixed with
It is clamped ring, the inner wall of the lateral surface and placing box that are clamped ring is slidably connected, is clamped on the inner wall of ring and is fixed with rubber pneumatic bag.
As a preferred technical solution of the present invention, the first gas nozzle is installed in the through-hole of the transverse slat upper surface, the
The gas outlet of one gas nozzle is connected to the air inlet of rubber pneumatic bag.
As a preferred technical solution of the present invention, the upper end of the placing box leading flank is fixed with fixing device, Gu
It includes fixed block to determine device, and the upper surface of fixed block is fixed with screw rod, and the upper end of screw rod is connected with nut thread, screw rod and side plate
The card slot of side is clamped, and the rear end of side plate is fixedly connected with the leading flank of transverse slat.
As a preferred technical solution of the present invention, the upper end of the cover board and one end of rotor plate are rotated by shaft
It connects, the first permanent magnet is fixed in the mounting hole of rotor plate, the position that babinet corresponds to the first permanent magnet is fixed with the second permanent magnetism
Iron.
As a preferred technical solution of the present invention, the side of the cover board leading flank is fixed with rubber ring, rubber ring
It is correspondingly arranged with the upper end of babinet.
As a preferred technical solution of the present invention, the quantity of the elastic device is four, and four elastic devices
It is uniformly distributed in the bottom surface of box house.
As a preferred technical solution of the present invention, the elastic device includes telescopic rod, the lower end of telescopic rod and case
The bottom surface in internal portion is fixedly connected, and the upper end of telescopic rod is fixedly connected with the lower surface of gas-permeable nets, and the side of telescopic rod is socketed with
Spring.
Compared with prior art, the beneficial effects of the invention are as follows：1) this autologous tissue stem cell applies constant temperature to preserve in vitro
Device, elastic device improve the damping performance of placing box, and rubber pneumatic bag prevents the collection bag equipped with mesenchymal stem cells from shaking, and protects
Demonstrate,prove the activity of mesenchymal stem cells.
2) when the temperature in babinet is less than set temperature, PLC controller controls wind turbine and semiconductor chilling plate work, wind
Machine makes the cold air that semiconductor chilling plate generates be uniformly distributed in babinet, and the temperature of babinet maintains setting range, improves
This autologous tissue stem cell applies the reliability of constant temperature save set in vitro.
3) PLC controller control air pump work so that maintain negative pressure close between babinet and cover board to ensure in babinet
Feng Xing, prevents that bacterium from entering in babinet and cover board is not easy to open.
4) card slot on side plate and screw rod clamping, the inner wall of the lateral surface and placing box that are clamped ring are slidably connected, Yi Shengxuan
Turn nut makes the position of side plate and transverse slat fix, and the collection bag good fixing effect of mesenchymal stem cells is housed.
Description of the drawings
Fig. 1 is main view cross-sectional view of the present invention；
Fig. 2 is right side structural representation of the present invention；
Fig. 3 is structure enlargement diagram at A of the present invention；
Fig. 4 is structure enlargement diagram at B of the present invention.
In figure：1 rubber ring, 2 rotor plates, 3 first permanent magnets, 4 cover boards, 5 ultraviolet radiator, 6 hinges, 7 fixing devices, 71 spiral shells
Mother, 72 screw rods, 73 fixed blocks, 8 elastic devices, 81 springs, 82 telescopic rods, 9 buckling devices, 91 transverse slats, 92 first gas nozzles, 93 cards
Connect ring, 94 rubber pneumatic bags, 10 placing boxes, 11 semiconductor chilling plates, 12 wind turbines, 13 temperature sensors, 14PLC controllers, 15 gas
Pump, 16 side plates, 17 second permanent magnets, 18 babinets, 19 card slots, 20 second gas nozzles, 21 gas-permeable nets.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 4 are please referred to Fig.1, the present invention provides a kind of technical solution：A kind of autologous tissue stem cell applies constant temperature to preserve in vitro
Device, including babinet 18, the rear side of 18 upper end of babinet and the lower end of cover board 4 are rotatablely connected by hinge 6, the upper end of cover board 4 and
One end of rotor plate 2 is rotatablely connected by shaft, is fixed with the first permanent magnet 3 in the mounting hole of rotor plate 2, and babinet 18 corresponds to the
The position of one permanent magnet 3 is fixed with the second permanent magnet 17, and the first permanent magnet 3 and the second permanent magnet 17 adsorb so that cover board 4 is fixed
Securely, the underrun elastic device 8 inside babinet 18 is fixedly connected with the lower surface of gas-permeable nets 21, the quantity of elastic device 8
It it is four, and bottom surface of four elastic devices 8 inside babinet 18 is uniformly distributed, elastic device 8 includes telescopic rod 82, telescopic rod
82 lower end is fixedly connected with the bottom surface inside babinet 18, and the upper end of telescopic rod 82 is fixedly connected with the lower surface of gas-permeable nets 21,
The side of telescopic rod 82 is socketed with spring 81, and spring 81 allows gas-permeable nets 21 to teetertotter, the side of gas-permeable nets 21 and case
The inner wall of body 18 is slidably connected, and the upper surface of gas-permeable nets 21 is fixed there are two placing box 10, and the upper surface of two placing boxes 10 is equal
Buckling device 9 is installed.
Buckling device 9 includes transverse slat 91, and the lower surface of transverse slat 91 is fixed with clamping ring 93, is clamped the lateral surface of ring 93 and puts
The inner wall for setting case 10 is slidably connected, and is clamped on the inner wall of ring 93 and is fixed with rubber pneumatic bag 94, pacifies in the through-hole of 91 upper surface of transverse slat
Equipped with the first gas nozzle 92, the gas outlet of the first gas nozzle 92 is connected to the air inlet of rubber pneumatic bag 94, and elastic device 8 improves placement
The damping performance of case 10, rubber pneumatic bag 94 prevent the collection bag equipped with mesenchymal stem cells from shaking, ensure the activity of mesenchymal stem cells.
The upper end of 10 leading flank of placing box is fixed with fixing device 7, and fixing device 7 includes fixed block 73, fixed block 73
Upper surface is fixed with screw rod 72, and the upper end of screw rod 72 and nut 71 are threadedly coupled, and the card slot 19 of 16 side of screw rod 72 and side plate blocks
It connects, the rear end of side plate 16 is fixedly connected with the leading flank of transverse slat 91, and fixing device 7 is so that the position of side plate 16 and transverse slat 91 is solid
It is fixed.
Bottom surface inside babinet 18 is fixed with air pump 15, and the gas outlet of air pump 15 and the air inlet of babinet 18 are connected by conduit
Logical, the air inlet of air pump 15 is connected to the air inlet of check valve by conduit, and the is fixed in the mounting hole of 18 right side of babinet
Two gas nozzles 20, the bottom surface inside babinet 18 are fixed with PLC controller 14, temperature sensor 13 and wind turbine 12,18 right side of babinet
The position of corresponding wind turbine 12 offers mounting hole, and semiconductor chilling plate 11, the system of semiconductor chilling plate 11 are fixed in mounting hole
Cold end is towards the inside of babinet 18, and when the temperature in babinet 18 is less than set temperature, PLC controller 14 controls wind turbine 12 and half
Conductor cooling piece 11 works, and wind turbine 12 makes the cold air that semiconductor chilling plate 11 generates be uniformly distributed in babinet 18, babinet
18 temperature maintains setting range, improves the reliability that this autologous tissue stem cell applies constant temperature save set in vitro, cover board 4
The side of leading flank is fixed with rubber ring 1, and the upper end of rubber ring 1 and babinet 18 is correspondingly arranged, and rubber ring 1 improves babinet 18 and lid
The leading flank of leakproofness between plate 4, cover board 4 is fixed with ultraviolet radiator 5, and ultraviolet radiator 5 works so that maintaining nothing in babinet 18
The output end electrical connection of collarium border, the input terminal of PLC controller 14 and external power supply, the output end of PLC controller 14 respectively with
The input terminal of air pump 15, the input terminal of semiconductor chilling plate 11, the input terminal of ultraviolet radiator 5 and wind turbine 12 input terminal be electrically connected
It connects, the output end electrical connection of the input terminal and temperature sensor 13 of PLC controller 14.
When in use：Collection bag equipped with mesenchymal stem cells is put into the inside of placing box 10 by doctor, and doctor makes side plate
Card slot 19 and screw rod 72 on 16 are clamped, and doctor makes the inner wall of the lateral surface and placing box 10 that are clamped ring 93 be slidably connected,
For doctor's rotating nuts 71 so that the position of side plate 16 and transverse slat 91 is fixed, doctor makes the gas outlet and first of external compression gas tank
The air inlet of gas nozzle 92 is connected to, and inflation makes the collection bag position equipped with mesenchymal stem cells fix in rubber pneumatic bag 94.
Doctor fastens cover board 4, and the first permanent magnet 3 and the second permanent magnet 17 adsorb and make 4 fixation of cover board, PLC
Controller 14 controls air pump 15 and works so that and maintain negative pressure to ensure the leakproofness between babinet 18 and cover board 4 in babinet 18,
Prevent that bacterium from entering in babinet 18 and cover board 4 is not easy to open；When needing to open cover board 4, doctor makes the second gas nozzle 20 lead
It is logical, restore normal barometric pressure in babinet 18, doctor makes the first permanent magnet 3 and the second permanent magnet 17 detach, doctor rotate the cover 4 from
And cover board 4 is opened.
When PLC controller 14 detects that the temperature in babinet 18 is less than set temperature by temperature sensor 13, PLC controls
Device 14 processed controls wind turbine 12 and semiconductor chilling plate 11 works, and wind turbine 12 makes the cold air that semiconductor chilling plate 11 generates in case
It is uniformly distributed in body 18.
The present invention, 1) elastic device 8 improves the damping performance of placing box 10, and rubber pneumatic bag 94 prevents dry thin equipped with mesenchymal
The collection bag of born of the same parents shakes, and ensures the activity of mesenchymal stem cells.
2) when the temperature in babinet 18 is less than set temperature, PLC controller 14 controls wind turbine 12 and semiconductor chilling plate
11 work, wind turbine 12 so that the cold air that semiconductor chilling plate 11 generates is uniformly distributed in babinet 18, tie up by the temperature of babinet 18
It holds and improves the reliability that this autologous tissue stem cell applies constant temperature save set in vitro in setting range.
3) PLC controller 14 controls air pump 15 and works so that maintains negative pressure to ensure babinet 18 and cover board 4 in babinet 18
Between leakproofness, prevent that bacterium from entering in babinet 18 and cover board 4 is not easy to open.
4) card slot 19 on side plate 16 and screw rod 72 are clamped, and the inner wall sliding for being clamped the lateral surface and placing box 10 of ring 93 connects
It connects, doctor's rotating nuts 71 make the position of side plate 16 and transverse slat 91 fix, and the collection bag fixed effect of mesenchymal stem cells is housed
It is good.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (10)
1. a kind of autologous tissue stem cell applies constant temperature save set, including babinet (18) in vitro, it is characterised in that：The babinet
(18) lower end of the rear side and cover board (4) of upper end is rotatablely connected by hinge (6), the internal underrun elasticity dress of babinet (18)
It sets (8) to be fixedly connected with the lower surface of gas-permeable nets (21), the side of gas-permeable nets (21) and the inner wall of babinet (18) are slidably connected, thoroughly
There are two placing boxes (10) for the upper surface fixation of gas net (21), and the upper surface of two placing boxes (10) is mounted on buckling device
(9), the internal bottom surface of babinet (18) is fixed with PLC controller (14), temperature sensor (13) and wind turbine (12), and babinet (18) is right
The position that side corresponds to wind turbine (12) offers mounting hole, and semiconductor chilling plate (11), semiconductor refrigerating are fixed in mounting hole
Towards the inside of babinet (18), the input terminal of PLC controller (14) and the output end of external power supply are electrically connected the refrigeration end of piece (11)
It connects, the input terminal of the output end of PLC controller (14) respectively with the input terminal of semiconductor chilling plate (11) and wind turbine (12) is electrically connected
It connects, the output end electrical connection of the input terminal and temperature sensor (13) of PLC controller (14).
2. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
The leading flank for stating cover board (4) is fixed with ultraviolet radiator (5), the output end of the input terminal and PLC controller (14) of ultraviolet radiator (5)
Electrical connection.
3. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
It states the internal bottom surface of babinet (18) and is fixed with air pump (15), the gas outlet of air pump (15) and the air inlet of babinet (18) pass through conduit
Connection, the air inlet of air pump (15) are connected to the air inlet of check valve by conduit, solid in the mounting hole of babinet (18) right side
Surely there are the second gas nozzle (20), the input terminal of air pump (15) and the output end electrical connection of PLC controller (14).
4. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
It includes transverse slat (91) to state buckling device (9), and the lower surface of transverse slat (91) is fixed with clamping ring (93), the lateral surface of clamping ring (93)
It is slidably connected with the inner wall of placing box (10), rubber pneumatic bag (94), transverse slat (91) upper table is fixed on the inner wall of clamping ring (93)
First gas nozzle (92) is installed, the gas outlet of the first gas nozzle (92) is connected to the air inlet of rubber pneumatic bag (94) in the through-hole in face.
5. a kind of autologous tissue stem cell according to claim 4 applies constant temperature save set in vitro, it is characterised in that：Institute
One end of the upper end and rotor plate (2) of stating cover board (4) is rotatablely connected by shaft, and is fixed in the mounting hole of rotor plate (2)
The position of one permanent magnet (3), corresponding first permanent magnet (3) of babinet (18) is fixed with the second permanent magnet (17).
6. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
The upper end for stating placing box (10) leading flank is fixed with fixing device (7), and fixing device (7) includes fixed block (73), fixed block
(73) upper surface is fixed with screw rod (72), and the upper end of screw rod (72) and nut (71) are threadedly coupled.
7. a kind of autologous tissue stem cell according to claim 6 applies constant temperature save set in vitro, it is characterised in that：Institute
Card slot (19) clamping of screw rod (72) and side plate (16) side is stated, the rear end of side plate (16) and the leading flank of transverse slat (91) are fixed and connected
It connects.
8. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
The side for stating cover board (4) leading flank is fixed with rubber ring (1), and the upper end of rubber ring (1) and babinet (18) is correspondingly arranged.
9. a kind of autologous tissue stem cell according to claim 1 applies constant temperature save set in vitro, it is characterised in that：Institute
The quantity for stating elastic device (8) is (four), and four elastic devices (8) are uniformly distributed in the internal bottom surface of babinet (18)；Institute
It includes telescopic rod (82) to state elastic device (8), and the bottom surface that the lower end of telescopic rod (82) is internal with babinet (18) is fixedly connected, and is stretched
The upper end of bar (82) is fixedly connected with the lower surface of gas-permeable nets (21), and the side of telescopic rod (82) is socketed with spring (81).
10. a kind of autologous tissue stem cell applies constant temperature store method in vitro, it is characterised in that：Doctor will be equipped with mesenchymal stem cells
Collection bag be put into the inside of placing box 10, doctor makes card slot 19 on side plate 16 and screw rod 72 be clamped, and doctor makes card
The inner wall of the lateral surface and placing box 10 that connect ring 93 is slidably connected, and doctor's rotating nuts 71 make the position of side plate 16 and transverse slat 91
Fixed, doctor makes the gas outlet of external compression gas tank be connected to the air inlet of the first gas nozzle 92, and inflation is swollen in rubber pneumatic bag 94
It is swollen that the collection bag position equipped with mesenchymal stem cells is fixed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810310657.6A CN108529012B (en) | 2018-04-09 | 2018-04-09 | Isolated application constant-temperature preservation device for autologous tissue stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810310657.6A CN108529012B (en) | 2018-04-09 | 2018-04-09 | Isolated application constant-temperature preservation device for autologous tissue stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108529012A true CN108529012A (en) | 2018-09-14 |
| CN108529012B CN108529012B (en) | 2019-12-20 |
Family
ID=63483393
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810310657.6A Expired - Fee Related CN108529012B (en) | 2018-04-09 | 2018-04-09 | Isolated application constant-temperature preservation device for autologous tissue stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108529012B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357740A (en) * | 2020-06-01 | 2020-07-03 | 广州赛太特生物医学科技有限公司 | Low-energy-consumption cell culture transfer heat-preservation box |
| CN112744429A (en) * | 2020-12-23 | 2021-05-04 | 河南理工大学 | Kit for detecting activity of anti-hand-foot-and-mouth disease drug |
| CN112913836A (en) * | 2021-04-21 | 2021-06-08 | 济南创泽生物医药科技有限公司 | A cold-storage plant for stem cell culture |
| CN113686072A (en) * | 2021-08-17 | 2021-11-23 | 王正昕 | Constant temperature refrigerator is transplanted to liver convenient to transport |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111357740A (en) * | 2020-06-01 | 2020-07-03 | 广州赛太特生物医学科技有限公司 | Low-energy-consumption cell culture transfer heat-preservation box |
| CN112744429A (en) * | 2020-12-23 | 2021-05-04 | 河南理工大学 | Kit for detecting activity of anti-hand-foot-and-mouth disease drug |
| CN112744429B (en) * | 2020-12-23 | 2022-07-22 | 河南理工大学 | Kit for detecting activity of anti-hand-foot-and-mouth disease drug |
| CN112913836A (en) * | 2021-04-21 | 2021-06-08 | 济南创泽生物医药科技有限公司 | A cold-storage plant for stem cell culture |
| CN112913836B (en) * | 2021-04-21 | 2022-02-01 | 济南创泽生物医药科技有限公司 | A cold-storage plant for stem cell culture |
| CN113686072A (en) * | 2021-08-17 | 2021-11-23 | 王正昕 | Constant temperature refrigerator is transplanted to liver convenient to transport |
| CN113686072B (en) * | 2021-08-17 | 2022-08-19 | 王正昕 | Constant temperature refrigerator is transplanted to liver convenient to transport |
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| CN108529012B (en) | 2019-12-20 |
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