CN108519329A - A kind of line co-focusing imaging device of multi-channel scanning and detection - Google Patents
A kind of line co-focusing imaging device of multi-channel scanning and detection Download PDFInfo
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention discloses the line co-focusing imaging devices of a kind of multi-channel scanning and detection, including：Excitation module, scanning imagery module and detecting module；For excitation module for providing the two linear light spots being separated from each other obtained by two beam laser beam reshapings, the inclination angle of laser beam and the angle of divergence are adjustable；Two lines hot spot for being focused on different image spaces by scanning imagery module, to generate two beam imaging beams, and realizes that 3-D scanning is imaged；Detecting module for realizing two-way imaging beam separation and detection；By fine-tuning the inclination angle of two-way light beam and the angle of divergence in excitation module, bifocal segment scanning imagery or single same focal plane two-wire of objective angular field while scanning imagery simultaneously may be implemented.The present invention can effectively improve image taking speed, while be convenient for system adjustment.
Description
Technical field
The invention belongs to micro-imaging fields, more particularly, to the line co-focusing imaging of a kind of multi-channel scanning and detection
Device.
Background technology
Micro-imaging technique is widely used in life science and medical domain, and image taking speed is compared in micro-imaging field
It is particularly important to obtain image information particularly with the imaging of substantially product sample in the short time for important parameter.According to exciting light
Shape, micro imaging method can be divided into spot scan, line scanning and Surface scan.The most common application of spot scan is copolymerized for laser scanning
Focusing microscope, this imaging method are just placed aperture and are interfered with filtering out non-focus plane information before the detectors, form optical chromatography, from
And improve picture contrast；Laser Scanning Confocal Microscope is point by point scanning sample to obtain complete image, and image taking speed is slow, is usually used in small
Imaging samples.Surface scan, that is, wide field imaging pattern, single exposure can obtain the signal of entire objective angular field, but by
In no chromatographic effect, so the signal between difference interferes with each other, it is difficult to obtain high-resolution image.Line is scanned into image space
Formula is a kind of imaging mode between the spot scan knead dough scanning imagery mode, it both can as Laser Scanning Confocal Microscope
Slit is added in detection light path, improves signal noise ratio (snr) of image, and sweep speed can be improved to a certain extent.
Existing line Laser Scanning Confocal Microscope generally generates linear light spot using cylindrical mirror, and using galvanometer as scanning device,
This kind of microscope is under 512 × 512 Pixel Dimensions of single objective angular field, and frame per second is up to 100Hz.But if will be to Centimeter Level substantially
Product sample is imaged, then needs to introduce three-dimensional mobile sample platform and sample is driven to carry out mosaic scanning imagery, due to platform from
During current location is moved to next image space, system is not imaged, and sample is bigger, platform move number it is more, it is non-at
The picture time is also more, affects image taking speed and imaging efficiency.
Invention content
In view of the drawbacks of the prior art and Improvement requirement, the present invention provides a kind of multi-channel scanning and the copolymerization of the line of detection are burnt
Imaging device realizes the burnt multichannel of line scanning copolymerization simultaneously it is intended that by redesigning illumination path and detection light path
Scanning and detection, to improve image taking speed and imaging efficiency.
To achieve the above object, the present invention provides the line co-focusing imaging devices of a kind of multi-channel scanning and detection, including：
Excitation module, scanning imagery module and detecting module；Excitation module is used to provide to be obtained by two beam laser beam reshapings
Two linear light spots being separated from each other, the inclination angle of laser beam and the angle of divergence are adjustable；Scanning imagery module is used for two lines
Hot spot focuses on different image spaces, to generate two beam imaging beams, and control sample in X direction, Y-direction and Z-direction
Movement is to realize that 3-D scanning is imaged；Detecting module for realizing two beam imaging beams separation and detection；Based on laser beam
The angle of divergence is adjustable, the linear light spot obtained through laser beam reshaping be focused after Z-direction position-adjustable；Inclining based on laser beam
Oblique angle is adjustable, the linear light spot obtained through laser beam reshaping be focused after X-direction position-adjustable；Wherein, X-direction is scanning
Direction, Z-direction are that sample is axial, and Y-direction is the distribution arrangement of imaging band in same focal plane, and X-direction, Y-direction, Z-direction
Constitute right-handed coordinate system.
Further, excitation module includes：Polarization splitting prism, cylindrical lens and two beam arrangements；Two light beams
For structure for providing two beam laser beams, the inclination angle and the angle of divergence per one laser beam are adjustable；Polarization splitting prism is used for
According to the polarization direction of laser beam transmission or reflection laser beam so that the direction of propagation of two beam laser beams is identical；Cylindrical lens are used for
The linear light spot that the identical two beams laser beam reshaping in the direction of propagation is separated from each other at two.
Further, beam arrangement includes that the laser set gradually along optical path direction, expand unit, inclination angle are adjusted
Unit and half-wave plate；Expand unit is used to expanding the laser beam that laser generates and adjusting the angle of divergence of laser beam,
To adjust the Z-direction position after linear light spot is focused；Inclination angle adjusts the inclination angle that unit is used to adjust laser beam, to adjust
Nodel line hot spot be focused after X-direction position；Half-wave plate for changing laser beam polarization direction so that two beam laser beams pass through
The direction of propagation is identical after polarization splitting prism.
Further, expand unit includes two convex lenses of common optical axis setting, the axially opposing position of two convex lenses
It sets adjustable；Expand unit expands method realization using Keplerian and expands, and expands than the ratio between the focal length for two convex lenses.
Further, it includes two speculums that inclination angle, which adjusts unit,.
Further, beam arrangement further includes speculum, for adjusting optical path direction.
Further, scanning imagery module includes：Dichroic mirror, object lens, D translation platform, optical filter and cylinder mirror；Two colors
Mirror is used to linear light spot reflecting into object lens, and selects the fluorescence generated through sample that fluorescence signal is made to enter a mirror；Object lens are used
In two lines hot spot to be focused on to different image spaces, excitation sample generates two-way fluorescence signal；Cylinder mirror and object lens common optical axis
Setting, the two constitute infinity correct microscope, and the fluorescence signal for being generated to sample is imaged, and form two-way imaging beam；
Optical filter is set between the object lens and cylinder mirror, for filtering out impurity signal, improves image quality；D translation platform is for making
Sample in X direction, Y-direction and Z-direction movement with realize 3-D scanning be imaged, surface is perpendicular to objective lens optical axis；In one kind
Under operating mode, the setting of excitation module makes the adjusting Jing Guo excitation module, two lines hot spot be focused on not through object lens
With focal plane and have dislocation in X-direction, with realize bifocal segment simultaneously scanning imagery and ensure two beam imaging beams can divide completely
From to improve image taking speed；And then sample moved by D translation platform, it can be achieved that the quick three-dimensional of substantially product sample at
Picture.
Further, include that the uniaxial galvanometer set gradually along optical path direction, scanning are saturating between dichroic mirror and object lens
Mirror, scanning drum mirror and the 4th speculum；Uniaxial galvanometer is for being scanned linear light spot to realize the imaging of entire visual field；It sweeps
Lens are retouched to relay for realizing light path with scanning drum mirror；4th speculum is for adjusting optical path direction；In one mode of operation,
The setting of excitation module so that adjusting Jing Guo excitation module, two lines hot spot through object lens focus on different focal planes and
X-direction has dislocation, with realize bifocal segment simultaneously scanning imagery and ensure two beam imaging beams can be kept completely separate, to improve at
As speed；And then by D translation platform movement sample, it can be achieved that the rapid three dimensional imaging to substantially product sample；At another
Under operating mode, the setting of excitation module makes the adjusting Jing Guo excitation module, two lines hot spot be focused on together by object lens
The image space that Y-direction position is identical in one focal plane and X-direction is at a distance of visual field apart from half, and realized by uniaxial vibration mirror scanning
Single same focal plane two-wire of objective angular field while scanning imagery improve image taking speed.
Further, detecting module includes：Right-angle prism, the first line-scan digital camera and the second line-scan digital camera；Right-angle prism
Two right-angle surfaces on be coated with reflectance coating, for two-way imaging beam to be detached, and make separation after imaging beam difference
It is detected by the first line-scan digital camera and the second line-scan digital camera；The test surface of first line-scan digital camera and the second line-scan digital camera is respectively positioned on scanning
In the image planes of image-forming module, and the first line-scan digital camera and the second line-scan digital camera are utilized respectively its test surface as line copolymerization coke
Slit improves picture contrast to filter out non-focal plane signal.
In general, contemplated above technical scheme through the invention, can obtain following advantageous effect：
(1) the line co-focusing imaging device of multi-channel scanning provided by the present invention and detection, by excitation module provide by
Two linear light spots being separated from each other that two beam laser beam reshapings obtain, by the angle of divergence and the inclination angle of adjusting laser beam so that line
Hot spot focuses to different image spaces by scanning imagery mould object lens in the block, to generate two beam imaging beams, and by detecting
Module realizes the separation and detection of two beam imaging beams.It can realize to two image spaces while scanning imagery, retain line
While being copolymerized burnt imaging advantage, the imaging flux in the unit interval is increased, sweep speed is effectively improved；
(2) the line co-focusing imaging device of multi-channel scanning provided by the present invention and detection, in its preferred embodiment, scanning
Image-forming module includes dichroic mirror, object lens, D translation platform, optical filter and cylinder mirror；The setting of excitation module makes through too drastic
The adjusting of light emitting module, two lines hot spot focus on different focal planes through object lens and have dislocation in X-direction, can ensure two beams
Bifocal segment scanning imagery simultaneously is realized while imaging beam can be kept completely separate, and improves image taking speed；And then it is flat by three-dimensional
Moving stage moves sample, it can be achieved that rapid three dimensional imaging to substantially product sample；
(3) the line co-focusing imaging device of multi-channel scanning provided by the present invention and detection, in its preferred embodiment, scanning
Image-forming module further includes uniaxial galvanometer；The setting of excitation module makes the adjusting Jing Guo excitation module, two lines hot spot warp
Object lens focus on different focal planes, bifocal segment can be realized while ensureing that two beam imaging beams can be kept completely separate while swept
Imaging is retouched, image taking speed is improved；And then sample moved by D translation platform, it can be achieved that the quick three-dimensional of substantially product sample at
Picture；Or the adjusting of excitation module being arranged Jing Guo excitation module, two lines hot spot focus on Y in same focal plane by object lens
The image space that direction position is identical and X-direction is at a distance of visual field apart from half, and then can be realized by uniaxial vibration mirror scanning single
The same focal plane two-wire of objective angular field while scanning imagery improve image taking speed；
(4) the line co-focusing imaging device of multi-channel scanning provided by the present invention and detection, the linear array phase of detecting module
When machine detection imaging light beam, according to line-scan digital camera test surface in the characteristic for there was only several pixels perpendicular to the direction of linear light spot, directly
It connects and is copolymerized burnt slit as line using its test surface, non-focal plane signal can either be filtered out, improve picture contrast, and convenient for being
System is adjusted.
Description of the drawings
Fig. 1 is the line co-focusing imaging schematic device of multi-channel scanning and detection that first embodiment of the invention provides；
Fig. 2 is the formation schematic diagram of center line hot spot of the embodiment of the present invention；(a) illustrate for the formation of YZ planes interior lines hot spot
Figure；(b) it is the schematic diagram of XZ planes interior lines hot spot；
Fig. 3 is that multi-route hot spot forms schematic diagram；
Fig. 4 is the scanning imagery mode of system；
Fig. 5 is the line co-focusing imaging schematic device of multi-channel scanning and detection that second embodiment of the invention provides；
In all the appended drawings, identical reference numeral is used for indicating identical element or structure, wherein：
1 is excitation module, and 2 be scanning imagery module, and 3 be detecting module, and 10 be laser, and 11 be the first convex lens,
12 be the second convex lens, and 13 be the first speculum, and 14 be the second speculum, and 15 be third speculum, and 16 be half-wave plate, and 17 be inclined
Shake Amici prism, and 18 be cylindrical lens, and 20 be dichroic mirror, and 21 be object lens, and 22 be sample, and 23 be D translation platform, and 24 be optical filter,
25 be cylinder mirror, and 26 be uniaxial galvanometer, and 27 be scanning lens, and 28 be scanning drum mirror, and 29 be the 4th speculum, and 30 be right-angle prism,
31 be the first line-scan digital camera, and 32 be the second line-scan digital camera.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
It does not constitute a conflict with each other and can be combined with each other.
The line co-focusing imaging device of multi-channel scanning provided by the invention and detection, as shown in Figure 1, in first embodiment
In, including：Excitation module 1, scanning imagery module 2 and detecting module 3；Excitation module 1 is for providing by two beam laser
Two linear light spots being separated from each other that beam shaping obtains, the inclination angle of laser beam and the angle of divergence are adjustable；Scanning imagery module 2
For two lines hot spot to be focused on to different image spaces, to generate two beam imaging beams, and control sample in X direction, Y
Direction and Z-direction movement are to realize that 3-D scanning is imaged；Detecting module 3 for realizing two beam imaging beams separation and detection；
The angle of divergence based on laser beam is adjustable, the linear light spot obtained through laser beam reshaping be focused after Z-direction position-adjustable；Base
It is adjustable in the inclination angle of laser beam, the linear light spot obtained through laser beam reshaping be focused after X-direction position-adjustable；Its
In, X-direction is scanning direction, and Z-direction is that sample is axial, and Y-direction is the distribution arrangement of imaging band in same focal plane, and the side X
To, Y-direction, Z-direction constitute right-handed coordinate system.
Excitation module 1 includes：Polarization splitting prism 17, cylindrical lens 18 and two beam arrangements；Two beam arrangements
For providing two beam laser beams, the inclination angle and the angle of divergence per one laser beam are adjustable；Polarization splitting prism 17 is used for root
According to the polarization direction transmission or reflection laser beam of laser beam so that the direction of propagation of two beam laser beams is identical；Cylindrical lens 18 are used for
The linear light spot that the identical two beams laser beam reshaping in the direction of propagation is separated from each other at two；Beam arrangement include along optical path direction according to
The laser 10 of secondary setting, expand unit, inclination angle adjust unit and half-wave plate 16；Expand unit is used to generate laser
Laser beam expanded and adjusted the angle of divergence of laser beam, to adjust the Z-direction position after linear light spot is focused；Inclination angle
The inclination angle that unit is used to adjust laser beam is adjusted, to adjust the X-direction position after linear light spot is focused；Half-wave plate 16 is used for
Change the polarization direction of laser beam so that two beam laser beam directions of propagation after polarization splitting prism are identical；Expand unit packet
The first convex lens 11 and the second convex lens 12 of common optical axis setting are included, the relative axial position of two convex lenses is adjustable；It expands
Unit expands method realization using Keplerian and expands, and expands than the ratio between the focal length for two convex lenses；Inclination angle adjusts unit
Including the first speculum 13 and the second speculum 14；In one of two beam arrangements beam arrangement, in the second speculum
Further include third speculum 15 between 14 and half-wave plate 16, for adjusting optical path direction.
Scanning imagery module 2 includes：Dichroic mirror 20, object lens 21, D translation platform 23, optical filter 24 and cylinder mirror 25；Two
Look mirror 20 is used to linear light spot reflecting into object lens 21, and selects the fluorescence generated through sample 22 that fluorescence signal is made to enter a mirror
25；Object lens 21 generate two-way fluorescence signal for two lines hot spot to be focused on to different image spaces, excitation sample 22；Cylinder mirror
25 are arranged with 21 common optical axis of object lens, and the two constitutes infinity correct microscope, and the fluorescence signal for being generated to sample 22 is imaged,
Form two-way imaging beam；Optical filter 24 is set between object lens 21 and cylinder mirror 25, for filtering out impurity signal, is improved into image quality
Amount；D translation platform for so that sample 22 in X direction, Y-direction and Z-direction movement with realize 3-D scanning be imaged, surface
Perpendicular to objective lens optical axis；In one mode of operation, the setting of excitation module 1 is so that adjusting Jing Guo excitation module 1, and two
Linear light spot has dislocation through poly- 21 coke of object lens to different focal planes and in X-direction, i.e., two lines hot spot after object lens 21 focus in X
Direction and Z-direction have dislocation, with realize bifocal segment simultaneously scanning imagery and ensure two beam imaging beams can be kept completely separate；Into
And by the movement sample 22 of D translation platform 23, it can be achieved that the rapid three dimensional imaging to substantially product sample；In this operating mode
Under, a linear light spot after the focusing of object lens 21, YZ planes and XZ planes formation respectively as shown in Fig. 2 (a) and Fig. 2 (b), two
The formation of road hot spot is as shown in Figure 3；When scanning imagery, as shown in figure 4, D translation platform 23 first drives sample 22 to move in X direction
It is dynamic, while realizing the imaging of a band in two focal planes；In focal plane after the completion of a Scan imaging, D translation platform 23
Sample 22 is driven to be moved along Y-direction, next band is scanned imaging in focusing plane；After the completion of entire focal plane scanning imagery,
D translation platform 23 drives sample 22 to be moved along Z-direction, and imaging is scanned to next group of focal plane.
Detecting module 3 includes：Right-angle prism 30, the first line-scan digital camera 31 and the second line-scan digital camera 32；Right-angle prism 30
Two right-angle surfaces on be coated with reflectance coating, for two-way imaging beam to be detached, and make separation after imaging beam difference
It is detected by the first line-scan digital camera 31 and the second line-scan digital camera 32；The test surface of first line-scan digital camera 31 and the second line-scan digital camera 32 is equal
In the image planes of scanning imagery module 2, and the first line-scan digital camera 31 and the second line-scan digital camera 32 are utilized respectively its test surface
The slit burnt as line copolymerization improves picture contrast to filter out non-focal plane signal.
The line co-focusing imaging device of multi-channel scanning provided by the invention and detection, as shown in figure 5, in second embodiment
In, include shaking along the single shaft that optical path direction is set gradually between dichroic mirror 20 and object lens 21 on the basis of first embodiment
Mirror 26, scanning lens 27, scanning drum mirror 28 and the 4th speculum 29；Uniaxial galvanometer 26 is for being scanned with reality linear light spot
The now imaging of entire visual field；Scanning lens 27 is relayed with scanning drum mirror 28 for realizing light path；4th speculum 29 is for adjusting
Optical path direction；In one mode of operation, the setting of excitation module 1 makes the adjusting Jing Guo excitation module 1, two lines light
Spot focuses on different focal planes through object lens 21 and has dislocation in X-direction, to realize bifocal segment scanning imagery and two beams of guarantee simultaneously
Imaging beam can be kept completely separate；And then sample 22 is moved, it can be achieved that the quick of substantially product sample by D translation platform 23
Three-dimensional imaging；Under another operating mode, the setting of excitation module 1 is so that adjusting Jing Guo excitation module 1, two
Linear light spot is focused on the image space that Y-direction position is identical in same focal plane and X-direction is at a distance of visual field apart from half by object lens 21,
And then it is scanned by uniaxial galvanometer 26 and realizes single same focal plane two-wire of objective angular field scanning imagery simultaneously.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, all within the spirits and principles of the present invention made by all any modification, equivalent and improvement etc., should all include
Within protection scope of the present invention.
Claims (8)
1. the line co-focusing imaging device of a kind of multi-channel scanning and detection, which is characterized in that including：Excitation module (1), scanning
Image-forming module (2) and detecting module (3)；
The excitation module (1) is described for providing the two linear light spots being separated from each other obtained by two beam laser beam reshapings
The inclination angle of laser beam and the angle of divergence are adjustable；The scanning imagery module (2) is used to two lines hot spot focusing on difference
Image space, to generate two beam imaging beams, and control sample in X direction, Y-direction and Z-direction movement to realize three-dimensional
Scanning imagery；The detecting module (3) for realizing two beam imaging beams separation and detection；The angle of divergence based on laser beam can
Adjust, the linear light spot obtained through laser beam reshaping be focused after Z-direction position-adjustable；Inclination angle based on laser beam is adjustable
Section, the linear light spot obtained through laser beam reshaping be focused after X-direction position-adjustable；
Wherein, X-direction is scanning direction, and Z-direction is that sample is axial, and Y-direction is the distribution arrangement of imaging band in same focal plane,
And X-direction, Y-direction, Z-direction constitute right-handed coordinate system.
2. the line co-focusing imaging device of multi-channel scanning as described in claim 1 and detection, which is characterized in that the exciting light
Module (1) includes：Polarization splitting prism (17), cylindrical lens (18) and two beam arrangements；Described two beam arrangements are used for
Two beam laser beams are provided, the inclination angle and the angle of divergence per one laser beam are adjustable；The polarization splitting prism (17) is used for
According to the polarization direction of laser beam transmission or reflection laser beam so that the direction of propagation of two beam laser beams is identical；The cylindrical lens
(18) the linear light spot for being used to the identical two beams laser beam reshaping in the direction of propagation being separated from each other at two.
3. the line co-focusing imaging device of multi-channel scanning as claimed in claim 2 and detection, which is characterized in that the light beam knot
Structure includes laser (10), expand unit, inclination angle adjusting unit and the half-wave plate set gradually along optical path direction；
The laser beam that the expand unit is used to generate the laser (10) is expanded and is adjusted the angle of divergence of laser beam,
To adjust the Z-direction position after linear light spot is focused；The inclination angle adjusts the inclination angle that unit is used to adjust laser beam, from
And adjust the X-direction position after linear light spot is focused；The half-wave plate (16) for changing laser beam polarization direction so that two
Beam laser beam direction of propagation after the polarization splitting prism (17) is identical.
4. the line co-focusing imaging device of multi-channel scanning as described in claim 1 and detection, which is characterized in that described to be scanned into
As module (2) includes：Dichroic mirror (20), object lens (21), D translation platform (23), optical filter (24) and cylinder mirror (25)；
The dichroic mirror (20) is used to linear light spot reflecting into object lens, and the fluorescence generated through sample is selected to make fluorescence signal
Into the cylinder mirror (25)；Two lines hot spot for being focused on different image spaces, excitation sample production by the object lens (21)
Raw two-way fluorescence signal；The cylinder mirror (25) is arranged with the object lens (21) common optical axis, and the two constitutes infinity correct microscope,
Fluorescence signal imaging for being generated to sample, forms two-way imaging beam；The optical filter (24) is set to the object lens
(21) between the cylinder mirror (25), for filtering out impurity signal, image quality is improved；The D translation platform (23) is for making
Sample in X direction, Y-direction and Z-direction movement with realize 3-D scanning be imaged, surface is perpendicular to objective lens optical axis.
5. the line co-focusing imaging device of multi-channel scanning as claimed in claim 4 and detection, which is characterized in that the exciting light
The setting of module (1) makes the adjusting by the excitation module (1), two lines hot spot be focused on not through the object lens (21)
With focal plane and have dislocation in X-direction, to realize bifocal segment simultaneously scanning imagery and ensure two beam imaging beams can be complete
Separation.
6. the line co-focusing imaging device of multi-channel scanning as claimed in claim 4 and detection, which is characterized in that in two color
Include the uniaxial galvanometer (26) set gradually along optical path direction between mirror (20) and the object lens (21), scanning lens (27), sweep
Retouch a mirror (28) and the 4th speculum (29)；
The single shaft galvanometer (26) is for being scanned linear light spot to realize the imaging of entire visual field；The scanning lens (27)
It is relayed for realizing light path with the scanning drum mirror (28)；4th speculum (29) is for adjusting optical path direction.
7. the line co-focusing imaging device of multi-channel scanning as claimed in claim 6 and detection, which is characterized in that the exciting light
The setting of module (1) makes the adjusting by the excitation module (1), two lines hot spot be focused on not through the object lens (21)
With focal plane and have dislocation in X-direction, with realize bifocal segment simultaneously scanning imagery and ensure two beam imaging beams can divide completely
From；Or the setting of the excitation module (1) makes the adjusting by the excitation module (1), two lines hot spot is by institute
It states object lens (21) and focuses on the image space that Y-direction position is identical in same focal plane and X-direction is at a distance of visual field apart from half, and lead to
It crosses the uniaxial galvanometer (26) and scans the single same focal plane two-wire of objective angular field of realization scanning imagery simultaneously.
8. the line co-focusing imaging device of multi-channel scanning as described in claim 1 and detection, which is characterized in that the detection mould
Block (3) includes：Right-angle prism (30), the first line-scan digital camera (31) and the second line-scan digital camera (32)；
It is coated with reflectance coating in two right-angle surfaces of the right-angle prism (30), for detaching two-way imaging beam, and is made
Imaging beam after separation is detected by first line-scan digital camera (31) and second line-scan digital camera (32) respectively；Described first
Line-scan digital camera (31) and the test surface of second line-scan digital camera (32) are respectively positioned in the image planes of the scanning imagery module (2),
And first line-scan digital camera (31) and second line-scan digital camera (32) are utilized respectively its test surface as line copolymerization coke
Slit improves picture contrast to filter out non-focal plane signal.
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| CN201810252673.4A CN108519329B (en) | 2018-03-26 | 2018-03-26 | Multi-channel scanning and detecting line confocal imaging device |
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| CN201810252673.4A CN108519329B (en) | 2018-03-26 | 2018-03-26 | Multi-channel scanning and detecting line confocal imaging device |
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| CN110824684A (en) * | 2019-10-28 | 2020-02-21 | 华中科技大学 | High-speed three-dimensional multi-modal imaging system and method |
| CN111012325A (en) * | 2019-12-05 | 2020-04-17 | 华中科技大学苏州脑空间信息研究院 | Precise light control system and light control method |
| CN111122568A (en) * | 2018-11-01 | 2020-05-08 | 华中科技大学苏州脑空间信息研究院 | High-flux optical tomography method and imaging system |
| CN113267495A (en) * | 2021-05-06 | 2021-08-17 | 华中科技大学苏州脑空间信息研究院 | Device and method for recombining every two parallel line light spots into collinear line light spots |
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| CN113267495A (en) * | 2021-05-06 | 2021-08-17 | 华中科技大学苏州脑空间信息研究院 | Device and method for recombining every two parallel line light spots into collinear line light spots |
| CN114217055A (en) * | 2021-12-02 | 2022-03-22 | 极瞳生命科技(苏州)有限公司 | Portable fluorescence scanning detection device and method |
| CN116754565A (en) * | 2023-08-04 | 2023-09-15 | 哈尔滨工业大学 | Automatic focusing detection method for optical element full-caliber surface micro-defect photo-induced fluorescence detection |
| CN116754565B (en) * | 2023-08-04 | 2024-04-26 | 哈尔滨工业大学 | Automatic focusing detection method for optical element full-caliber surface micro-defect photo-induced fluorescence detection |
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