CN108517341A - A kind of preparation process of elastin laminin - Google Patents
A kind of preparation process of elastin laminin Download PDFInfo
- Publication number
- CN108517341A CN108517341A CN201810369136.8A CN201810369136A CN108517341A CN 108517341 A CN108517341 A CN 108517341A CN 201810369136 A CN201810369136 A CN 201810369136A CN 108517341 A CN108517341 A CN 108517341A
- Authority
- CN
- China
- Prior art keywords
- elastin laminin
- ligament
- preparation process
- solution
- semi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000016942 Elastin Human genes 0.000 title claims abstract description 34
- 108010014258 Elastin Proteins 0.000 title claims abstract description 34
- 229920002549 elastin Polymers 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 210000003041 ligament Anatomy 0.000 claims abstract description 27
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000008363 phosphate buffer Substances 0.000 claims abstract description 6
- 230000000694 effects Effects 0.000 claims abstract description 5
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000020 Nitrocellulose Substances 0.000 claims description 3
- 229920001220 nitrocellulos Polymers 0.000 claims description 3
- 238000000527 sonication Methods 0.000 claims description 3
- 238000002604 ultrasonography Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims 7
- 108091005804 Peptidases Proteins 0.000 claims 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 239000003814 drug Substances 0.000 abstract description 3
- 238000002525 ultrasonication Methods 0.000 abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 2
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 102000034240 fibrous proteins Human genes 0.000 description 3
- 108091005899 fibrous proteins Proteins 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000003321 cartilage cell Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 239000000806 elastomer Substances 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000000887 hydrating effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 206010025135 lupus erythematosus Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 210000000107 myocyte Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000002798 polar solvent Substances 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000009938 salting Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a kind of preparation processes of elastin laminin, include the following steps：The first step is uniformly mixed to obtain ligament solution with phosphate buffer after ligament to be cleaned to rubbing, and elastoser is added in ligament solution；Sodium chloride is added in ligament solution after addition elastoser by second step, then carries out ultrasonication and shake culture；Third step by multilayer filtered through gauze and discards filter residue, and filtrate is passed through semi-permeable membrane after activated carbon decolorizing；4th step, semi-permeable membrane is filtered after elastin laminin collect after be dissolved in pure water, supernatant is taken after centrifugation；Supernatant obtained by 4th step is placed in ultrafilter and passes through semi-permeable membrane by the 5th step.Elastin laminin prepared by a kind of preparation process of elastin laminin of the present invention it is pure, activity is high, is highly suitable for the numerous areas such as food, medicine, makeup.
Description
Technical field
The present invention relates to field of material preparation, especially a kind of preparation process of elastin laminin.
Background technology
Elastin laminin is a kind of scleroprotein being present in ligament and blood vessel wall, is mainly put down by fibroblast, main artery
The sliding generations such as myocyte and cartilage cell, can be combined to form elastomer with microfibril, major function is passive in vivo
Flexible, tissue and organ are with retractility and reversible deformability where assigning.The content of elastin laminin in the tissue is because of elasticity
Function is different to the difference of the significance level of tissue, content highest in ligament, accounts for about 80%, is followed successively by aorta 30% thereafter
~50%, lung 10%~25%, skin 2%~3%.Elastin laminin is a kind of extremely poorly soluble scleroprotein, insoluble in diluted acid,
Only there is Swelling in some polar solvents in alkali, alcohol and salting liquid；Elastin laminin just has elasticity under hydrating condition
Can, and have certain ductility.
Elastin laminin decides the elasticity and flexibility of skin, can prevent aging and promote regenerated effect.In addition, bullet
Property albumen belonging to scleroprotein play a part of supporting body and protection that body is resistance to is hit in animal body.Human body lacks hard
Albumen easilys lead to connective tissue lesion, and it is crisp that hemorrhoid, lupus erythematosus, chorionitis, psoriasis, leucoderma, capillary occurs
Property increase, wound it is refractory close etc. illnesss.
Currently, the market demand of elastin laminin is growing day by day, but the preparation process of elastin laminin and not perfect, it is therefore desirable to
A kind of efficient elastin laminin preparation process meets market needs.
Invention content
In order to overcome the defects of the prior art, a kind of preparation process of elastin laminin is provided.
The present invention is realized by following proposal：
A kind of preparation process of elastin laminin, includes the following steps：
The first step is uniformly mixed to obtain ligament solution with phosphate buffer after ligament to be cleaned to rubbing, in ligament solution
Elastoser is added；
8.5gNaCl will be added in the every 1L solution of ligament solution after addition elastoser in second step, is then surpassed
Sonicated, and shake culture 2h under the conditions of 25 DEG C；
Third step, by second step, treated that ligament solution by multilayer filtered through gauze and discards filter residue, and filtrate is passed through
Activated carbon decolorizing, which is placed in ultrafilter, passes through semi-permeable membrane；
4th step, semi-permeable membrane is filtered after elastin laminin collect after be dissolved in pure water, adjust pH value to 9.5, stand
30min centrifuges 15min under conditions of rotating speed is 15000r/min, takes supernatant；
Supernatant obtained by 4th step is placed in ultrafilter and passes through semi-permeable membrane by the 5th step.
In the second step, the ultrasound intensity of ultrasonication is 0.4W/cm2, frequency 50KHz, power 140W,
Sonication treatment time is 20min.
In the second step, the rotating speed of shake culture is 100~120r/min.
In third step, it is 5.05 × 10 that the ligament solution in ultrafilter, which is in air pressure,5The condition of Pa
It is lower to pass through semi-permeable membrane.
In the 5th step, it is 5.05 × 10 that the supernatant in ultrafilter, which is in air pressure,5Under conditions of Pa
Under pass through semi-permeable membrane.
The semi-permeable membrane is nitrocellulose filter.
Environment temperature in the third step, the 4th step and the 5th step is 4 DEG C.
In the first step, the phosphate buffer pH value is 7.8 ± 0.5.
In the first step, the addition of elastoser is per addition 1g elastoser in 100g ligament solution.
Beneficial effects of the present invention are：
Elastin laminin prepared by a kind of preparation process of elastin laminin of the present invention it is pure, activity is high, is highly suitable for
The numerous areas such as food, medicine, makeup.
Specific implementation mode
The preferred embodiment of the invention is further illustrated with reference to specific embodiment：
A kind of preparation process of elastin laminin, includes the following steps：
The first step is uniformly mixed to obtain ligament solution with phosphate buffer after ligament to be cleaned to rubbing, in ligament solution
Elastoser is added；The phosphate buffer pH value is 7.8 ± 0.5.The addition of elastoser is molten per 100g ligaments
1g elastoser is added in liquid.
8.5gNaCl will be added in the every 1L solution of ligament solution after addition elastoser in second step, is then surpassed
Sonicated, and shake culture 2h under the conditions of 25 DEG C；The ultrasound intensity of ultrasonication is 0.4W/cm2, frequency is
50KHz, power 140W, sonication treatment time 20min.The rotating speed of shake culture is 100~120r/min.
Third step, by second step, treated that ligament solution by multilayer filtered through gauze and discards filter residue, and filtrate is passed through
Activated carbon decolorizing, which is placed in ultrafilter, passes through semi-permeable membrane；Ligament solution in ultrafilter is to be in air pressure
5.05×105Pass through semi-permeable membrane under under conditions of Pa.
4th step, semi-permeable membrane is filtered after elastin laminin collect after be dissolved in pure water, adjust pH value to 9.5, stand
30min centrifuges 15min under conditions of rotating speed is 15000r/min, takes supernatant；
Supernatant obtained by 4th step is placed in ultrafilter and passes through semi-permeable membrane by the 5th step.It is upper in ultrafilter
It is 5.05 × 10 that clear liquid, which is in air pressure,5Pass through semi-permeable membrane under under conditions of Pa.
In practical applications, the environment temperature in the third step, the 4th step and the 5th step is 4 DEG C to the present invention.It is described
Semi-permeable membrane is nitrocellulose filter.Chemical constitution, the preparation and application of elastoser, semi-permeable membrane in the present invention be
Existing known technology, here, repeating no more.
Elastin laminin prepared by a kind of preparation process of elastin laminin of the present invention it is pure, activity is high, is highly suitable for
The numerous areas such as food, medicine, makeup.
Although having done more detailed elaboration to technical scheme of the present invention and having enumerated, it should be understood that for ability
For field technique personnel, modification is made to above-described embodiment or uses equivalent alternative solution, this is to those skilled in the art
It is it is clear that these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the present invention for member
Claimed range.
Claims (9)
1. a kind of preparation process of elastin laminin, which is characterized in that include the following steps：
The first step is uniformly mixed to obtain ligament solution with phosphate buffer after ligament to be cleaned to rubbing, is added in ligament solution
Elastoser；
8.5gNaCl will be added in the every 1L solution of ligament solution after addition elastoser in second step, then carries out ultrasonic wave
Processing, and shake culture 2h under the conditions of 25 DEG C；
Third step, by second step, treated that ligament solution by multilayer filtered through gauze and discards filter residue, by filtrate by activity
Carbon decoloring, which is placed in ultrafilter, passes through semi-permeable membrane；
4th step, semi-permeable membrane is filtered after elastin laminin collect after be dissolved in pure water, adjust pH value to 9.5, stand 30min,
15min is centrifuged under conditions of rotating speed is 15000r/min, takes supernatant；
Supernatant obtained by 4th step is placed in ultrafilter and passes through semi-permeable membrane by the 5th step.
2. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In the second step, surpass
The ultrasound intensity of sonicated is 0.4W/cm2, frequency 50KHz, power 140W, sonication treatment time 20min.
3. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In the second step, shake
The rotating speed for swinging culture is 100~120r/min.
4. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In third step, surpass
It is 5.05 × 10 that ligament solution in filter device, which is in air pressure,5Pass through semi-permeable membrane under under conditions of Pa.
5. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In the 5th step, surpass
It is 5.05 × 10 that supernatant in filter device, which is in air pressure,5Pass through semi-permeable membrane under under conditions of Pa.
6. a kind of preparation process of elastin laminin according to any one claim in claim 1,4 and 5, special
Sign is：The semi-permeable membrane is nitrocellulose filter.
7. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：The third step, the 4th step
It it is 4 DEG C with the environment temperature in the 5th step.
8. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In the first step, the phosphorus
Acid buffer pH value is 7.8 ± 0.5.
9. a kind of preparation process of elastin laminin according to claim 1, it is characterised in that：In the first step, elasticity
The addition of protease is per addition 1g elastoser in 100g ligament solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369136.8A CN108517341A (en) | 2018-04-23 | 2018-04-23 | A kind of preparation process of elastin laminin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369136.8A CN108517341A (en) | 2018-04-23 | 2018-04-23 | A kind of preparation process of elastin laminin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108517341A true CN108517341A (en) | 2018-09-11 |
Family
ID=63429056
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810369136.8A Pending CN108517341A (en) | 2018-04-23 | 2018-04-23 | A kind of preparation process of elastin laminin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108517341A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109680031A (en) * | 2019-03-08 | 2019-04-26 | 福建康是美生物科技有限公司 | A kind of extracting method of Elastin peptide |
| CN110664666A (en) * | 2019-11-06 | 2020-01-10 | 上海海洋大学 | Multi-effect cream for promoting skin fibroblasts to secrete structural proteins |
| CN111171139A (en) * | 2020-02-21 | 2020-05-19 | 海南美肽生物科技有限公司 | Elastin and synthetic method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6284025A (en) * | 1985-10-09 | 1987-04-17 | Ichimaru Fuarukosu Kk | Production of hydrolyzed placenta-insoluble protein having fibroblast cell proliferation promoting action |
| US20090281044A1 (en) * | 2003-02-14 | 2009-11-12 | Thomas Mitts | Elastin digest compositions and methods utilizing same |
| CN102241747A (en) * | 2011-06-17 | 2011-11-16 | 李立文 | Humanlike elastin and production method thereof |
| CN106381323A (en) * | 2016-10-26 | 2017-02-08 | 无限极（中国）有限公司 | Elastin peptide as well as preparation method and application thereof |
| CN107435060A (en) * | 2017-09-29 | 2017-12-05 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation method of longan nuclear protein polypeptide |
-
2018
- 2018-04-23 CN CN201810369136.8A patent/CN108517341A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6284025A (en) * | 1985-10-09 | 1987-04-17 | Ichimaru Fuarukosu Kk | Production of hydrolyzed placenta-insoluble protein having fibroblast cell proliferation promoting action |
| US20090281044A1 (en) * | 2003-02-14 | 2009-11-12 | Thomas Mitts | Elastin digest compositions and methods utilizing same |
| CN102241747A (en) * | 2011-06-17 | 2011-11-16 | 李立文 | Humanlike elastin and production method thereof |
| CN106381323A (en) * | 2016-10-26 | 2017-02-08 | 无限极（中国）有限公司 | Elastin peptide as well as preparation method and application thereof |
| CN107435060A (en) * | 2017-09-29 | 2017-12-05 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of preparation method of longan nuclear protein polypeptide |
Non-Patent Citations (2)
| Title |
|---|
| 张春红等: "《食品酶制剂及应用》", 30 June 2008, 中国计量出版社 * |
| 梅星元等: "《生物化学》", 31 August 2007, 华中师范大学出版 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109680031A (en) * | 2019-03-08 | 2019-04-26 | 福建康是美生物科技有限公司 | A kind of extracting method of Elastin peptide |
| CN110664666A (en) * | 2019-11-06 | 2020-01-10 | 上海海洋大学 | Multi-effect cream for promoting skin fibroblasts to secrete structural proteins |
| CN111171139A (en) * | 2020-02-21 | 2020-05-19 | 海南美肽生物科技有限公司 | Elastin and synthetic method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107929816B (en) | Anti-adhesion material with hemostatic, antibacterial and healing promoting functions and preparation method thereof | |
| US20210100830A1 (en) | Starch-based hemostatic powder and preparation method thereof | |
| CN108517341A (en) | A kind of preparation process of elastin laminin | |
| CN105597156B (en) | Hydrogel and its preparation method and application | |
| JPS5988424A (en) | Hemostatic method, fiber proliferation inhibition and tissueregeneration promotion for tissue wound | |
| JP2014138890A (en) | Biocompatible modifed starch material capable of hemostasis, adhesion prevention, fusion promotion and surgical sealing | |
| JPS6333400A (en) | Collagen treatment method for easily dissolving crosslinking of collagen and collagen obtained | |
| KR20010102231A (en) | Hyaluronic acid gel composition, process for producing the same, and medical material containing the same | |
| CN104740685A (en) | Nerve repairing film and preparation method thereof | |
| CN111759858B (en) | PH-sensitive digestive tract mucosa protective gel and application thereof | |
| CN1157569A (en) | Anti-adhesion agent | |
| BR112020015616B1 (en) | BIOINK COMPOSITION FOR DERMIS REGENERATION SHEET, METHOD FOR MANUFACTURING CUSTOM DERMIS REGENERATION SHEET USING THE SAME AND CUSTOM DERMIS REGENERATION SHEET MANUFACTURED USING THE MANUFACTURING METHOD | |
| CN112121238A (en) | Multifunctional composite anti-adhesion material and preparation method thereof | |
| JP2016528007A (en) | Tissue repair device using self-assembled materials | |
| CN108367093A (en) | For stopping blooding, tissue barrier, wound healing and beauty the carboxymethyl cellulose-based matter of biocompatibility (BCM) | |
| JP2022546512A (en) | Hydrogel composition for temperature-sensitive tissue adhesion prevention and method for producing the same | |
| CN110038162B (en) | Functional silk fibroin material with function of regulating and controlling growth of vascular cells and preparation method thereof | |
| JP2004097033A (en) | Method for producing placental extract | |
| Angaria et al. | Natural polymer-based hydrogels: versatile biomaterials for biomedical applications | |
| CN110711264B (en) | Composite material, medical adhesive, and preparation method and application thereof | |
| CN108452366B (en) | Cod skin gelatin composite hemostatic dressing and preparation method thereof | |
| CN106139237A (en) | A kind of new and effective compound hemostatic sponge and preparation method thereof | |
| CN108969797A (en) | A kind of load lipidosome gel and preparation method thereof with electro photoluminescence and the neural scar effect of inhibition | |
| CN108084466A (en) | A kind of composite membrane that fluidized polymer is derived based on egg white and methacrylic acid and its application in terms of stem cell is cultivated | |
| CN104524603B (en) | It can be by the virus removal/ablation method for hemostasis biological products/biomaterial that living organism absorbs |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180911 |