CN108507623A - The environment network of silver ion content is detected based on gold nano grain - Google Patents
The environment network of silver ion content is detected based on gold nano grain Download PDFInfo
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- CN108507623A CN108507623A CN201810567662.5A CN201810567662A CN108507623A CN 108507623 A CN108507623 A CN 108507623A CN 201810567662 A CN201810567662 A CN 201810567662A CN 108507623 A CN108507623 A CN 108507623A
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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Abstract
The invention discloses the environment networks that silver ion content is detected based on gold nano grain, including host node computer (10), are connect for wireless signal between the host node computer (10) and multiple sensors of default type；Multiple sensors of the default type are mounted in the heat distribution pipeline (3) being laid in the underground space (100) and water supply line (6) and on the side wall of the underground space；Multiple sensors of the default type are used to acquire the detection data of the corresponding underground space.The environment network disclosed by the invention that silver ion content is detected based on gold nano grain, its can the environment of safe and reliable ground-to-ground down tube net carry out comprehensive monitoring, the content of silver ion is effectively detected in the water that can be especially transmitted to underground pipe network, the security risk of underground pipe network can be reduced, ensure the safe handling of underground pipe network, be conducive to widely apply, be of great practical significance.
Description
Technical field
The present invention relates to Underground Space Environment monitoring technical field, more particularly to based on gold nano grain detection silver from
The environment network of sub- content.
Background technology
Currently, underground pipe network is the most important thing in Chinese Urbanization construction process, it is to play city function, it is ensured that city
City's economy, Community health, the important substance of coordinated development are basic, and intricate, mutually overlapping stereoscopic features, city is presented
City's underground pipe network is the important component of urban infrastructure construction, is city energy conveying, mass transfer, an information
The important carrier that transmission, water drainage mitigation and waste dump.
Currently, when the environment water in underground pipe network is contaminated, it is easy to dramatically increase containing for the silver ion in water body
Amount.Silver ion is extremely toxic substance, and the toxic action having includes mainly：(1) protein and various enzymes for making human body become
Property.(2) the internal silver ion of entrance is more than 0.8g will cause the silver-colored spot of blue to precipitate on the skin.Since silver ion has
Very strong oxidisability, so can also cause the symptoms such as internal organs oedema into the silver ion in human body.Cause people dead when serious
It dies.Human body is without effectively arranging silver-colored mechanism.So once intake silver ion is just mainly accumulated in bone and liver.And silver from
Neutron poisoning does not have effective antidote.Thus, the silver ion content in natural water is it in weight in environment measuring control
Weight, to ensure human health, there is an urgent need to develop quick, sensitive, selected method to detect silver ion.
For current underground pipe network, the problem of being primarily present, is：Since the space structure of underground pipe network is complicated, nothing
Method carries out comprehensive monitoring to the environment of underground pipe network, and silver ion contains in the water that can not be especially transmitted to underground pipe network
Amount is effectively detected, and to reduce the security risk of underground pipe network, ensures the safe handling of underground pipe network.
Therefore, at present there is an urgent need to develop going out a kind of technology, can safe and reliable ground-to-ground down tube net environment into
The comprehensive monitoring of row especially can effectively detect the content of silver ion in the water of underground pipe network transmission, can be subtracted
The security risk of few underground pipe network, ensures the safe handling of underground pipe network.
Invention content
In view of this, the object of the present invention is to provide the environment network for detecting silver ion content based on gold nano grain,
Can the environment of safe and reliable ground-to-ground down tube net carry out comprehensive monitoring, the water that underground pipe network can be especially transmitted
The content of middle silver ion is effectively detected, and the security risk of underground pipe network can be reduced, and ensures that the safety of underground pipe network makes
With being conducive to widely apply, be of great practical significance.
For this purpose, the present invention provides the environment network for detecting silver ion content based on gold nano grain, including host node meter
Calculation machine connects between the host node computer and multiple sensors of default type for wireless signal；
Multiple sensors of the default type be mounted on the underground space in be laid with heat distribution pipeline and water supply line in
And on the side wall of the underground space；
Multiple sensors of the default type are used to acquire the detection data of the corresponding underground space.
Wherein, multiple sensors of the default type include：First temperature sensor, second flow sensor and table
Face enhances Raman SERS water quality sensors；
First temperature sensor, second flow sensor and surface-enhanced Raman SERS water quality sensors are mounted on
In the water supply line, for detecting the temperature of water, flow and silver ion content in the water supply line, it is then sent to
Host node computer.
Wherein, multiple sensors of the default type further include：First pressure sensor, first flow sensor and
Second temperature sensor；
The first pressure sensor, first flow sensor and second temperature sensor are mounted on the heat distribution pipeline
In, pressure, flow and temperature for detecting institute's transmission medium in the heat distribution pipeline are then sent to host node computer.
Wherein, multiple sensors of the default type further include：Oxygen sensor, carbon monoxide transducer, hydrogen sulfide
Sensor, Temperature Humidity Sensor and NOx sensor；
The oxygen sensor, carbon monoxide transducer, hydrogen sulfide sensor, Temperature Humidity Sensor and nitrogen oxides pass
Sensor is separately mounted on the side wall of the underground space, for collecting the oxygen gas concentration in the underground space, CO gas
Concentration, sulfureted hydrogen gas concentration, temperature, humidity and nitrous oxides concentration are then sent to host node computer.
Wherein, further include：First monitoring camera and the second monitoring camera；
First monitoring camera is located at the upper right side of the second monitoring camera；
First monitoring camera and the second monitoring camera are mounted on the side wall of the underground space, are respectively used to adopt
The image for collecting institute overlay area, is then sent to host node computer.
Wherein, the host node computer, including：Data memory module, data processing module and wireless data transmission mould
Block, wherein：
Data memory module, the detection data of the underground space for acquiring multiple sensors of the default type,
It is stored in real time；
Data processing module is connected with data memory module, for acquiring multiple sensors of the default type
The underground space detection data, be compared respectively with preset, corresponding normal value range, if positioned at it is preset,
Within corresponding normal value range, then corresponding data qualifier is judged, otherwise, then judge corresponding defect of data, together
When, comparable situation is sent to the computer of ground base station by wireless data transfer module in real time；
Data processing module is additionally operable to first monitoring camera and the second monitoring camera acquired image,
The computer of ground base station is sent to by wireless data transfer module.
Wherein, further include：Crusing robot, the crusing robot are arranged between heat distribution pipeline and water supply line
On ground.
Wherein, the surface-enhanced Raman SERS water quality sensors include kit and detection switch, wherein：
Detection switch is mounted on the water supply line；
The kit is located at the lower section of detection switch, and the top of the kit has multiple holes；
Include inserted with surface-enhanced Raman SERS chips, the surface-enhanced Raman SER S chips in the kit
Silicon dioxide substrates are arranged at intervals with multiple gold nanos modified by DNA and 2- thionaphthols in the silicon dioxide substrates
Grain distributed areas；
Each gold nano grain distributed areas by the modification of DNA and 2- thionaphthols are correspondingly arranged with a hole and phase
Connection；
The kit is arranged above a fibre-optical probe, and the fibre-optical probe passes through an optical fiber and one
Portable Raman detector is connected；
It is provided with antenna in the portable Raman detector.
Wherein, the preparation of the surface-enhanced Raman SERS chips includes the following steps：
The first step, the chlorauric acid solution ebuillition of heated with vigorous stirring for being 0.01% by mass concentration, in stirring
Under be rapidly added 1% trisodium citrate aqueous solution, the volume ratio of the chlorauric acid solution and trisodium citrate aqueous solution is
100:3；
Second step continues to heat and boil 15 minutes, so that lurid solution is gradually become stable peony, then will
Manufactured liquid is cooled to room temperature, and obtains gold colloid；
The 2- thionaphthols of a concentration of 0.01mM of molal volume are added to gold colloid solution mixing third step, are gently mixed
10 minutes, the gold nanoparticle separating liquid of 2- thionaphthols label is obtained, is then turned according to the centrifugation of the speed of 8000 rev/min
It is 10 minutes dynamic, remove supernatant, wherein the volume ratio of the 2- thionaphthols and gold colloid solution is 1:100；
4th step uses the phosphate buffer of a concentration of 10mM of 1mL to suspend again, the gold colloid solution after being suspended,
In, the volume ratio of phosphate buffer and gold colloid solution is 1：1；
Preset DNA solution is added in the gold colloid solution after suspending 5th step, reacts 12 hours, is then added
The sodium chloride solution salinization of a concentration of 0.1M of molal volume 30 minutes, then be placed in 4 degrees Celsius of temperature and continue salinization 6 hours,
Obtain mixture, wherein the volume ratio of 2- thionaphthols and sodium chloride solution is 1:2；
6th step centrifuges mixture 30 minutes under 8000 rpms of rotating speed, removes supernatant, is subsequently placed in
It suspends in the phosphate buffer of a concentration of 10mM of molal volume, obtains the gold nano grain by the modification of DNA and 2- thionaphthols
Solution；
7th step will be coated in silicon dioxide substrates by the gold nano grain of DNA and 2- thionaphthols modification, then set
It is dried in drying box, finally forms the gold nano grain by the modification of DNA and 2- thionaphthols in silicon dioxide substrates
Distributed areas prepare surface-enhanced Raman SERS chips.
Wherein, in the 5th step, the specific preparation process of the preset DNA solution is as follows：
DNA (CCCCCACCTCCCACCCACC) solution and molal volume of a concentration of 100uM of molal volume is a concentration of
Three (2- carboxyethyls) phosphonium salt acid salt solutions of 10mM mix, wherein the body of DN solution As and three (2- carboxyethyls) phosphonium salt acid salt solutions
Product is than being 20:3, and the volume ratio of DNA solution and the 2- thionaphthols in third step is 1:1, room temperature stands activation 1 hour, obtains
Obtain preset D NA solution.
By the above technical solution provided by the invention as it can be seen that compared with prior art, the present invention provides based on Jenner
Rice grain detect silver ion content environment network, can safe and reliable ground-to-ground down tube net environment carry out it is comprehensive
Monitoring especially can effectively detect the content of silver ion in the water of underground pipe network transmission, can reduce underground pipe network
Security risk, ensure the safe handling of underground pipe network, be conducive to widely apply, be of great practical significance.
Description of the drawings
Fig. 1 is the structural schematic diagram of the environment network provided by the invention that silver ion content is detected based on gold nano grain；
Fig. 2 is that the environment network provided by the invention that silver ion content is detected based on gold nano grain is based on purple honeybee
The data transmission schematic diagram that ZigBee wireless communication techniques are formed；
Fig. 3 is that surface enhanced is drawn in the environment network provided by the invention for detecting silver ion content based on gold nano grain
The cooperating state simplified schematic diagram of graceful (SERS) water quality sensor and water supply line；
Fig. 4 is that surface enhanced is drawn in the environment network provided by the invention for detecting silver ion content based on gold nano grain
The structure schematic diagram of graceful (SERS) chip；
Fig. 5 is gold nano grain in the environment network provided by the invention for detecting silver ion content based on gold nano grain
Attachment structure schematic diagram between DNA chain；
Fig. 6 is that surface enhanced is drawn in the environment network provided by the invention for detecting silver ion content based on gold nano grain
The Raman signal schematic diagram that graceful (SERS) chip obtains when being detected to liquid；
In figure, 1 is the first monitoring camera；2 be the second monitoring camera；3 be heat distribution pipeline；4 sense for first pressure
Device；5 be the first temperature sensor；6 be water supply line；7 be crusing robot；8 be first flow sensor；9 be second flow
Sensor；10 be main node computer；11 be second temperature sensor；12 be surface-enhanced Raman (SERS) water quality sensor；
13 be oxygen sensor；14 be carbon monoxide transducer；15 be hydrogen sulfide sensor；16 be Temperature Humidity Sensor；17 be nitrogen oxygen
Compound sensor；
18 be detection switch；19 be surface-enhanced Raman (SERS) chip；20 be kit；21 examine for portable Raman
Survey instrument；22 be antenna；23 be fibre-optical probe；24 be the gold nano grain distributed areas modified by DNA and 2- thionaphthols；25
For silicon dioxide substrates；26 be gold nano grain；27 be DNA chain.
Specific implementation mode
In order to make those skilled in the art replace understand the present invention program, below in conjunction with the accompanying drawings with embodiment pair
The present invention is described in further detail.
Referring to Fig. 1 to Fig. 6, the present invention provides the environment networks that silver ion content is detected based on gold nano grain, including
It is between multiple sensors (being used as minor node) of host node computer 10, the host node computer 10 and default type
Wireless signal (especially by purple honeybee ZigBee wireless communication techniques) connects；
Multiple sensors of the default type are mounted on the heat being laid in the underground space 100 (i.e. underground pipe network space)
In hydraulic piping 3 and water supply line 6 and on the side wall of the underground space；
Multiple sensors of the default type are used to acquire the detection data of the corresponding underground space, for example, temperature passes
Sensor is used to detect the temperature data of institute's installation site.
In the present invention, in specific implementation, multiple sensors of the default type include：First temperature sensor 5,
Second flow sensor 9 and surface-enhanced Raman SERS water quality sensors 12；
First temperature sensor 5, second flow sensor 9 and surface-enhanced Raman SERS water quality sensors 12 are pacified
In the water supply line 6, for detecting the temperature of water, flow and silver ion content in the water supply line 6, then
It is sent to host node computer 10 (especially by purple honeybee ZigBee wireless communication techniques).
In the present invention, in specific implementation, multiple sensors of the default type further include：First pressure sensor
4, first flow sensor 8 and second temperature sensor 11；
The first pressure sensor 4, first flow sensor 8 and second temperature sensor 11 are mounted on the heating power
In pipeline 3, pressure, flow and temperature for detecting institute's transmission medium (such as hot water or steam) in the heat distribution pipeline 3
Degree, is then sent to host node computer 10 (especially by purple honeybee Z igBee wireless communication techniques, such as ZigBee ad hoc network).
It should be noted that heat distribution pipeline is the transmission pipeline of high-temperature gas or high-temp liquid, for example, it is warm in city
Feed channel.
In the present invention, in specific implementation, multiple sensors of the default type further include：Oxygen sensor 13, one
Aoxidize carbon sensor 14, hydrogen sulfide sensor 15, Temperature Humidity Sensor 16 and NOx sensor 17；
The oxygen sensor 13, carbon monoxide transducer 14, hydrogen sulfide sensor 15, Temperature Humidity Sensor 16 and nitrogen
Oxide sensor 17 is separately mounted on the side wall of the underground space, is received for the integrated environment information to the underground space
Collection is specifically used for collecting oxygen gas concentration, carbon monoxide gas concentration, sulfureted hydrogen gas concentration, temperature in the underground space
Degree, humidity and nitrous oxides concentration are then sent to host node computer 10 and (are wirelessly communicated especially by purple honeybee ZigBee
Technology, such as ZigBee ad hoc network).
In the present invention, in specific implementation, Underground Space Environment provided by the invention monitors network, further includes：First prison
Control camera 1 and the second monitoring camera 2；
First monitoring camera is located at the upper right side of the second monitoring camera 2；
First monitoring camera, 1 and second monitoring camera 2 is mounted on the side wall of the underground space, is respectively used to
The image for acquiring institute overlay area is then sent to host node computer 10 and (wirelessly communicates skill especially by purple honeybee ZigBee
Art, such as ZigBee ad hoc network), institute overlay area is monitored in real time to realize.
In specific implementation, first monitoring camera, 1 and second monitoring camera 2 can be monitoring gunlock.
It should be noted that in specific implementation, first monitoring camera 1 can be with 15 meters of pipe network entrance located underground
Locate furred ceiling, supports 360 degree of infrared real time monitoring.18 sides meter Chu of second monitoring camera, 2 pipe network entrance located underground
Wall can monitor institute overlay area in real time.
It should be noted that for the present invention, in multiple sensors of the default type, each sensor wraps
Include power supply module, central control module, environment acquisition module and wireless communication module.
For the present invention, the host node computer 10, including：Data memory module, data processing module and without line number
According to transmission module, wherein：
Data memory module, the underground for acquiring multiple sensors (i.e. as minor node) of the default type
The detection data in space, is stored in real time, to form database；
Data processing module is connected with data memory module, is used for multiple sensors of the default type (i.e.
As minor node) detection data of the underground space of acquisition, it is (such as default with preset, corresponding normal value range respectively
Temperature value range, preset humidity range) be compared, if be located at preset, corresponding normal value range
Within, then judge corresponding data qualifier, otherwise, then judges corresponding defect of data, meanwhile, comparable situation is led in real time
Cross the computer that wireless data transfer module is sent to ground base station so that the staff on ground is recognized that underground
Every environmental parameter situation in space, obtains the environmental information of the underground space, realizes the real time monitoring of underground pipe network.In addition,
Data processing module is additionally operable to, by 1 and second monitoring camera of the first monitoring camera, 2 acquired image, pass through nothing
Line data transmission module is sent to the computer of ground base station.
For the present invention, in order to which more preferably ground-to-ground down space is monitored, Underground Space Environment network provided by the invention
Further include：Crusing robot 7, the crusing robot 7 are arranged on the ground between heat distribution pipeline 3 and water supply line 6.
It should be noted that the crusing robot 7 is mainly used for carrying out the security information monitoring of underground utilities, collect
The security information of underground utilities.The bottom of the crusing robot have wheel, can be equipped with thereon infrared thermography,
Visible image capturing head and laser navigation module (existing laser navigation module)；
Wherein, by infrared thermography, temperature change can be detected, so as to monitor heat distribution pipeline 3 in real time and supply
The leakage problem of the underground utilities such as waterpipe 6；
Visible image capturing head monitors in real time for the image to the underground space, for ensureing that pipeline performance has safely
Important role.
Laser navigation module, the route planning for ensureing crusing robot 7 and intelligent detecting.
It should be noted that the crusing robot 7 is used to move and carry out the agent structure of environmental monitoring, it is existing
The structure of crusing robot be not unfolded to describe herein herein similarly to the prior art.
In specific implementation, the data memory module, data processing module and wireless data transfer module are located at host node
The lower part of computer 10.
It should be noted that for the present invention, the data of all minor nodes can be transmitted to master by zigbee network
In node computer, data are collected, handle, compare and are retrieved by each data module of host node computer, most
The environmental information that will be obtained afterwards can be transferred out by zigbee wireless communication modules.It is adopted by the data of whole system
Collection is stored, handles and is transmitted, and realizes the comprehensive utilization of underground pipe gallery information, is the overall planning of the underground space and comprehensive tune
Degree provides strong data supporting.
As shown in figure 3, for the present invention, surface-enhanced Raman (SERS) water quality sensor 12 includes kit 20
With detection switch 18, wherein：
Detection switch 18 is mounted on the water supply line 6；It is specifically as follows an electromagnetic switch valve；
The kit 20 is located at the lower section of detection switch 18, and the top of the kit 20 has multiple holes；
Inserted with surface-enhanced Raman (SERS) chip 19, the surface-enhanced Raman (SERS) in the kit 20
Chip 19 includes silicon dioxide substrates 25, is arranged at intervals in the silicon dioxide substrates 25 multiple by DNA and 2- thionaphthols
The gold nano grain distributed areas 24 of modification；
Each gold nano grain distributed areas 24 and a hole by the modification of DNA and 2- thionaphthols are correspondingly arranged simultaneously
It is connected, the liquid flowed into from hole can be connected to；
The kit 20 is arranged above a fibre-optical probe 23, and the fibre-optical probe 23 passes through an optical fiber
It is connected with a portable Raman detector 21；
Antenna 22 (being specifically as follows purple honeybee ZigBee antennas) is provided in the portable Raman detector 21.
In specific implementation, the portable Raman detector 21 can be a kind of existing portable Raman detector.Example
Such as, it may refer to Chinese utility model patent application No. is CN201621206981.6, being announced on November 9th, 2016
Application《A kind of portable multi-function Raman detector》The portable Raman detector that prospectus is recorded.
Therefore, it for the present invention, due to installing detection switch 18 below water supply line 6, when starting detection, opens
Detection switch 18 will instill in the SERS detection chips 19 in kit 20 from the dropping liquid drop that water supply line 6 flows out, to
It reacts, different pollutant monitorings will be instilled in different holes and are detected.Fibre-optical probe 23 can send out swashing for 532nm
Light carries out Raman detection and is handled in portable Raman detector 21, finally detects portable Raman detector 21
Silver ion content data radio to host node computer 10 by antenna 22 in the water of acquisition.
For the present invention, it should be noted that surface-enhanced Raman (SERS) water quality sensor 12 is mainly used in water silver-colored
The detection of ion, the principle utilized, which is silver ion, to form " C-Ag2+-C " structure with the C bases in DNA, pass through SERS skills
Art carrys out the presence of indirect detection silver ion.It, can be by 5 terminal modified mercaptos since sulfydryl can form stable Au-S keys with nanogold
Base and the DNA (DNA sequence dna CCCCCACCTCCCACCCACC) for being rich in C bases, which are modified, arrives gold nano grain surface.
In order to form the gold nano grain distributed areas by the modification of DNA and 2- thionaphthols in silicon dioxide substrates 25
24, surface-enhanced Raman (SERS) chip 19 is prepared, the gold nano particle modification process specifically carried out includes following step
Suddenly：
The first step, the chlorauric acid solution ebuillition of heated with vigorous stirring for being 0.01% by mass concentration, in stirring
Under be rapidly added 1% trisodium citrate aqueous solution, the volume ratio of the chlorauric acid solution and trisodium citrate aqueous solution is
100:3；
Second step continues to heat and boil 15 minutes, so that lurid solution is gradually become stable peony, then will
Manufactured liquid is cooled to room temperature, and obtains gold colloid solution.At this time, gold colloid can be stored in black bottle at 4 DEG C
In it is spare.
In specific implementation, when the chlorauric acid solution is 50mL, corresponding trisodium citrate aqueous solution is 1.5mL, corresponding
The diameter of gold colloid solution can be 30nm.
Third step, by 2- thionaphthols (2N T) addition of a concentration of 0.01mM of molal volume (i.e. mM every liter) to gold size
Liquid solution mix, be gently mixed 10 minutes, obtain 2- thionaphthols label gold nanoparticle separating liquid, then according to 8000 turns/
Speed centrifugation rotation per minute 10 minutes, removes supernatant, wherein the body of 2- thionaphthols (2NT) and gold colloid solution
Product is than being 1:100；
In specific implementation, when the volume of the 2- thionaphthols (2NT) is 10uL, corresponding gold colloid solution needs 1mL.
4th step uses the phosphate buffer (i.e. PBS, pH value 7) of a concentration of 10mM of 1mL to suspend again, after being suspended
Gold colloid solution, wherein the volume ratio of phosphate buffer and gold colloid solution be 1：1；
In specific implementation, when gold colloid solution is 1mL, selected phosphate buffer is also 1m L.
Preset DNA solution is added in the gold colloid solution after suspending 5th step, reacts 12 hours, is then added
The sodium chloride solution salinization of a concentration of 0.1M of molal volume (i.e. mole every liter) 30 minutes, then it is placed in 4 degrees Celsius of temperature relaying
Continuous salinization 6 hours, obtains mixture, wherein the volume ratio of 2- thionaphthols (2NT) and sodium chloride solution is 1:2；
In the 5th step, in specific implementation, when the volume of the 2- thionaphthols (2NT) is 10uL, sodium chloride solution
Volume is 20uL.
In specific implementation, the specific preparation process of the preset DNA solution is as follows：
By DNA (DNA sequence dna the is CCCCCACCTCCCAC CCACC) solution of a concentration of 100uM of molal volume and mole body
Three (2- carboxyethyls) phosphonium salt hydrochlorate (TCEP) solution mixing of a concentration of 10mM of product, wherein DNA solution and three (2- carboxyethyls) phosphines
The volume ratio of hydrochloride (TCEP) solution is 20:3, and the volume ratio of DNA solution and the 2- thionaphthols (2NT) in third step
It is 1:1, then room temperature, which is stood, activates 1 hour, obtains preset DNA solution.For example, when DNA solution is 1 0uL, three (2-
Carboxyethyl) phosphonium salt hydrochlorate (TCEP) solution be 1.5uL.
6th step centrifuges mixture 30 minutes under 8000 rpms of rotating speed, removes supernatant, is subsequently placed in
It suspends, is obtained by the modification of DNA and 2- thionaphthols in the phosphate buffer (pH value 7) of a concentration of 10mM of molal volume
Gold nano grain solution；
7th step will be coated in silicon dioxide substrates by the gold nano grain of DNA and 2- thionaphthols modification, then set
It is dried in drying box, finally forms the gold nano by the modification of DNA and 2- thionaphthols in silicon dioxide substrates 25
Grain distributed areas 24, prepare surface-enhanced Raman (SERS) chip 19.
As shown in figure 5, being prepared by the above method, any one gold nano grain 26 and adjacent gold nano grain 26
Between be closely joined together by DNA chain 27.
As shown in fig. 6, abscissa is Raman shift, wave-number difference of the light relative to incident light is exactly scattered, ordinate is light
Sub-count exactly scatters the intensity of light.In the presence of having silver ion in the drop instilled, gold nano grain passes through " C-Ag2+-
C " structures make gold nano grain reunite, and due to quantum hot spot-effect after reunion, generate strong Raman signal, feature
Peak is predominantly located at 1380cm-1Place, obtains concentration of silver ions, and transmit a signal to host node computer by the power of signal
10 are handled, in this way can be achieved drinking water silver ion 1pg/mL (every milliliter of pik) quick in situ monitoring, high-precision and
Real-time property in situ, ensures the safety of Drinking Water for Residents, to ensure the safe operation of underground pipe network.
In conclusion compared with prior art, it is provided by the invention that silver ion content is detected based on gold nano grain
Environment network, can the environment of safe and reliable ground-to-ground down tube net carry out comprehensive monitoring, especially can be to underground
The content of silver ion is effectively detected in the water of pipe network transmission, can reduce the security risk of underground pipe network, ensures buried pipe
The safe handling of net is conducive to widely apply, be of great practical significance.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. detecting the environment network of silver ion content based on gold nano grain, which is characterized in that including host node computer (10),
It is connect for wireless signal between the host node computer (10) and multiple sensors of default type；
Multiple sensors of the default type are mounted on the heat distribution pipeline (3) and water supply line being laid in the underground space (100)
(6) in and on the side wall of the underground space；
Multiple sensors of the default type are used to acquire the detection data of the corresponding underground space.
2. environment network as described in claim 1, which is characterized in that multiple sensors of the default type include：First
Temperature sensor (5), second flow sensor (9) and surface-enhanced Raman SERS water quality sensors (12)；
First temperature sensor (5), second flow sensor (9) and surface-enhanced Raman SERS water quality sensors (12) peace
In the water supply line (6), temperature, flow and silver ion content for detecting water in the water supply line (6), so
After be sent to host node computer (10).
3. environment network as claimed in claim 2, which is characterized in that multiple sensors of the default type further include：The
One pressure sensor (4), first flow sensor (8) and second temperature sensor (11)；
The first pressure sensor (4), first flow sensor (8) and second temperature sensor (11) are mounted on the heating power
In pipeline (3), pressure, flow and temperature for detecting institute's transmission medium in the heat distribution pipeline (3) are then sent to main section
Point computer (10).
4. environment network as claimed in claim 3, which is characterized in that multiple sensors of the default type further include：Oxygen
Gas sensor (13), carbon monoxide transducer (14), hydrogen sulfide sensor (15), Temperature Humidity Sensor (16) and nitrogen oxides pass
Sensor (17)；
The oxygen sensor (13), carbon monoxide transducer (14), hydrogen sulfide sensor (15), Temperature Humidity Sensor (16) and
NOx sensor (17) is separately mounted on the side wall of the underground space, dense for collecting the oxygen gas in the underground space
Degree, carbon monoxide gas concentration, sulfureted hydrogen gas concentration, temperature, humidity and nitrous oxides concentration, are then sent to host node
Computer (10).
5. environment network as claimed in claim 4, which is characterized in that further include：First monitoring camera (1) and the second monitoring
Camera (2)；
First monitoring camera is located at the upper right side of the second monitoring camera (2)；
First monitoring camera (1) and the second monitoring camera (2) are mounted on the side wall of the underground space, are respectively used to adopt
The image for collecting institute overlay area, is then sent to host node computer (10).
6. environment network as claimed in claim 5, which is characterized in that the host node computer (10), including：Data store
Module, data processing module and wireless data transfer module, wherein：
Data memory module, the detection data of the underground space for acquiring multiple sensors of the default type, in real time
It is stored；
Data processing module is connected with data memory module, the ground for acquiring multiple sensors of the default type
The detection data of down space is compared with preset, corresponding normal value range respectively, if positioned at preset, corresponding
Normal value range within, then judge corresponding data qualifier, otherwise, then judge corresponding defect of data, meanwhile, in real time
Comparable situation is sent to the computer of ground base station by wireless data transfer module；
Data processing module is additionally operable to first monitoring camera (1) and the second monitoring camera (2) acquired image,
The computer of ground base station is sent to by wireless data transfer module.
7. such as environment network according to any one of claims 1 to 6, which is characterized in that further include：Crusing robot (7), institute
It states on the ground that crusing robot (7) is arranged between heat distribution pipeline (3) and water supply line (6).
8. environment network as claimed in claim 2, which is characterized in that the surface-enhanced Raman SERS water quality sensors (12)
Including kit (20) and detection switch (18), wherein：
Detection switch (18) is mounted on the water supply line (6)；
The kit (20) is located at the lower section of detection switch (18), and the top of the kit (20) has multiple holes；
Inserted with surface-enhanced Raman SERS chips (19), the surface-enhanced Raman SERS chips in the kit (20)
(19) include silicon dioxide substrates (25), be arranged at intervals on the silicon dioxide substrates (25) multiple by DNA and 2- naphthalene sulphur
The gold nano grain distributed areas (24) of phenol modification；
Each gold nano grain distributed areas (24) by the modification of DNA and 2- thionaphthols are correspondingly arranged and are connected with a hole
It is logical；
The kit (20) is arranged above a fibre-optical probe (23), and the fibre-optical probe (23) passes through an optical fiber
It is connected with a portable Raman detector (21)；
It is provided with antenna (22) on the portable Raman detector (21).
9. environment network as claimed in claim 8, which is characterized in that the preparation of the surface-enhanced Raman SERS chips includes
Following steps：
The first step, the chlorauric acid solution ebuillition of heated with vigorous stirring for being 0.01% by mass concentration, it is fast under stirring
The volume ratio of the trisodium citrate aqueous solution of speed addition 1%, the chlorauric acid solution and trisodium citrate aqueous solution is 100:3；
Second step continues to heat and boil 15 minutes, and lurid solution is made to gradually become stable peony, then will be manufactured
Liquid is cooled to room temperature, and obtains gold colloid；
The 2- thionaphthols of a concentration of 0.01mM of molal volume are added to gold colloid solution mixing third step, are gently mixed 10 points
Clock obtains the gold nanoparticle separating liquid of 2- thionaphthols label, then according to 10 points of the speed of 8000 rev/min centrifugation rotation
Clock removes supernatant, wherein the volume ratio of the 2- thionaphthols and gold colloid solution is 1:100；
4th step uses the phosphate buffer of a concentration of 10mM of 1mL to suspend again, the gold colloid solution after being suspended, wherein phosphorus
The volume ratio of phthalate buffer and gold colloid solution is 1：1；
Preset DNA solution is added in the gold colloid solution after suspending 5th step, reacts 12 hours, then addition mole
The sodium chloride solution salinization that volumetric concentration is 0.1M 30 minutes, then be placed in 4 degrees Celsius of temperature and continue salinization 6 hours, it obtains
Mixture, wherein the volume ratio of 2- thionaphthols and sodium chloride solution is 1:2；
6th step centrifuges mixture 30 minutes under 8000 rpms of rotating speed, removes supernatant, is subsequently placed in a mole body
It suspends in the phosphate buffer of a concentration of 10mM of product, obtains the gold nano grain solution by the modification of DNA and 2- thionaphthols；
7th step will be coated in silicon dioxide substrates by the gold nano grain of DNA and 2- thionaphthols modification, then be placed in drying
It is dried in case, finally forms the gold nano grain distributed area by the modification of DNA and 2- thionaphthols in silicon dioxide substrates
Domain 24 prepares surface-enhanced Raman SERS chips 19.
10. environment network as claimed in claim 9, which is characterized in that in the 5th step, the tool of the preset DNA solution
Preparation step is as follows：
By DNA (CCCCCACCTCCCACCCACC) solution of a concentration of 100uM of molal volume with a concentration of 10mM's of molal volume
Three (2- carboxyethyls) phosphonium salt acid salt solutions mix, and the volume ratio of wherein DNA solution and three (2- carboxyethyls) phosphonium salt acid salt solutions is
20:3, and the volume ratio of DNA solution and the 2- thionaphthols in third step is 1:1, room temperature stands activation 1 hour, is preset
DNA solution.
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