CN108497057A - Composite coating preservative of the chromocor extract containing tartary buckwheat bran and its preparation method and application - Google Patents
Composite coating preservative of the chromocor extract containing tartary buckwheat bran and its preparation method and application Download PDFInfo
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- CN108497057A CN108497057A CN201810263238.1A CN201810263238A CN108497057A CN 108497057 A CN108497057 A CN 108497057A CN 201810263238 A CN201810263238 A CN 201810263238A CN 108497057 A CN108497057 A CN 108497057A
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- Prior art keywords
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- tartary buckwheat
- buckwheat bran
- chromocor extract
- carboxymethyl chitosan
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- 244000130270 Fagopyrum tataricum Species 0.000 title claims abstract description 54
- 235000014693 Fagopyrum tataricum Nutrition 0.000 title claims abstract description 54
- 239000000284 extract Substances 0.000 title claims abstract description 49
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N flavone Chemical compound O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 239000003755 preservative agent Substances 0.000 title claims abstract description 37
- 239000002131 composite material Substances 0.000 title claims abstract description 36
- 230000002335 preservative effect Effects 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000011248 coating agent Substances 0.000 title claims abstract description 8
- 238000000576 coating method Methods 0.000 title claims abstract description 8
- 229920001661 Chitosan Polymers 0.000 claims abstract description 59
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims abstract description 45
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 235000011187 glycerol Nutrition 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 34
- 239000000243 solution Substances 0.000 claims description 34
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
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- 238000005265 energy consumption Methods 0.000 abstract description 2
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- 230000004580 weight loss Effects 0.000 description 4
- JMGZEFIQIZZSBH-UHFFFAOYSA-N Bioquercetin Natural products CC1OC(OCC(O)C2OC(OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5)C(O)C2O)C(O)C(O)C1O JMGZEFIQIZZSBH-UHFFFAOYSA-N 0.000 description 3
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 235000009508 confectionery Nutrition 0.000 description 3
- IVTMALDHFAHOGL-UHFFFAOYSA-N eriodictyol 7-O-rutinoside Natural products OC1C(O)C(O)C(C)OC1OCC1C(O)C(O)C(O)C(OC=2C=C3C(C(C(O)=C(O3)C=3C=C(O)C(O)=CC=3)=O)=C(O)C=2)O1 IVTMALDHFAHOGL-UHFFFAOYSA-N 0.000 description 3
- FDRQPMVGJOQVTL-UHFFFAOYSA-N quercetin rutinoside Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 FDRQPMVGJOQVTL-UHFFFAOYSA-N 0.000 description 3
- 235000005493 rutin Nutrition 0.000 description 3
- IKGXIBQEEMLURG-BKUODXTLSA-N rutin Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](C)O[C@@H]1OC[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](OC=2C(C3=C(O)C=C(O)C=C3OC=2C=2C=C(O)C(O)=CC=2)=O)O1 IKGXIBQEEMLURG-BKUODXTLSA-N 0.000 description 3
- ALABRVAAKCSLSC-UHFFFAOYSA-N rutin Natural products CC1OC(OCC2OC(O)C(O)C(O)C2O)C(O)C(O)C1OC3=C(Oc4cc(O)cc(O)c4C3=O)c5ccc(O)c(O)c5 ALABRVAAKCSLSC-UHFFFAOYSA-N 0.000 description 3
- 229960004555 rutoside Drugs 0.000 description 3
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
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- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
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- 235000017173 flavonoids Nutrition 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000002736 nonionic surfactant Substances 0.000 description 2
- 239000004014 plasticizer Substances 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000005068 transpiration Effects 0.000 description 2
- 241000167854 Bourreria succulenta Species 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 241000219050 Polygonaceae Species 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
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- 239000013543 active substance Substances 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
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- 230000000259 anti-tumor effect Effects 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
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- 230000003138 coordinated effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
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- -1 flavone compound Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 150000004001 inositols Chemical class 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 238000005457 optimization Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/16—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cosmetics (AREA)
Abstract
The present invention discloses composite coating preservative of the chromocor extract containing tartary buckwheat bran and its preparation method and application.Composite coating preservative is composed of the following raw materials by weight：1-4 parts of tartary buckwheat bran chromocor extract, 10-25 parts of carboxymethyl chitosan, 30-40 parts of glycerine, 0.1-0.5 parts of Tween-20,1000 parts of water.High-pressure pulse electric extractive technique according to the present invention belongs to non-heated processing, have many advantages, such as to transmit uniformly quickly, processing time is short, heat production be few, low energy consumption, a variety of injuries that can overcome hot-working that may bring.The present invention improves the homogeneity of composite preservative film, while enhancing the integrality of film forming by adding the coalescents such as qs glycerin, it does not crack after so that film is air-dried, not only have good choke, resistance moist, also there is excellent anti-oxidant, fungistatic effect, and fresh-keeping effect is apparent；Each component of the present invention is environmentally protective, good water solubility, easy cleaning, nontoxic secondary residual.
Description
Technical field
The present invention relates to a kind of composite preservatives, more particularly to contain tartary buckwheat bran with anti-oxidant, notable antagonistic property
The composite coating preservative and the preparation method and application thereof of skin chromocor extract, belongs to fresh agricultural products technical field of preservation of fresh.
Background technology
Bitter buckwheat category polygonaceae, Fagopyrum, also known as hull buckwheat, which are that China is distinctive, to be suitable for growing in short season under Cold and cool climate
Dicotyledon.Bitter buckwheat nourishing also contains compared with horn of plenty in addition to containing protein, fat, vitamin and some trace elements
The small-molecule active substances such as flavonoids and inositol class, it is known that its wheat bran is used as feed always.But research has shown that hardship
Contain a large amount of flavone compound, such as rutin, Quercetin in buckwheat bran.This kind of Flavonoid substances remove free radical,
Pharmacological action, radiation resistance, anticancer and it is antitumor etc. be widely used, therefore tartary buckwheat bran is developed and utilized,
It therefrom extracts natural anti-oxidation, bacteriostasis efficacy ingredient and is applied to the fresh-keeping of the fresh agricultural products such as fruits and vegetables, not only open
The new way that tartary buckwheat bran efficiently utilizes, at the same be also fresh agricultural products wide spectrum, efficiently, the exploitation of natural bacteriostatic antistaling agent carries
New approaches are supplied, to realizing that the safety of the fresh agricultural products such as fruits and vegetables, preservative have important practical significance.
Fresh agricultural products are fresh-keeping at present mostly uses chemical fresh-keeping method greatly, and chemical fresh-keeping method toxicity is big, causes residual serious,
Not easy-clear, safety are under suspicion；In addition most of coating-film fresh-keeping generally only serves choke, hinders wet effect, lacks
Inhibit the multi-functional fresh-keeping effect of surface microorganism activity etc..
Invention content
Present invention aims to overcome that problem of the existing technology, it is a kind of movable with inhibition surface microorganism to provide,
Composite coating preservative of the chromocor extract containing tartary buckwheat bran safe to use and its preparation method and application is bitter by changing
The addition of buckwheat bran chromocor extract significantly affects anti-oxidant, the bacteriostasis property of the film.
The present invention by by from tartary buckwheat bran chromocor extract and water-soluble good carboxymethyl chitosan it is compound,
There is provided it is a kind of it is novel with antioxidant activity, high-efficiency broad spectrum is antibacterial and multifunctional green without any toxicity and side effects is naturally compound
Coating antistaling agent will have broad application prospects.
The present invention in order to achieve the above object used by technical solution be：
A kind of composite preservative containing tartary buckwheat bran chromocor extract, is composed of the following raw materials by weight：Duck wheat
1-4 parts of wheat bran chromocor extract, 10-25 parts of carboxymethyl chitosan, 30-40 parts of glycerine, 0.1-0.5 parts of Tween-20, water 1000
Part.
To further realize the object of the invention, it is preferable that the tartary buckwheat bran chromocor extract uses following methods system
：
(1) respectively as unit of g and mL, by dry tartary buckwheat bran and ethanol solution according to solid-liquid ratio 1：(30‐50)
Mixing is uniformly mixed and is placed on high-pressure pulse electric, is 12-16mL/min, umber of pulse 10-14 in circulation, electric field strength is
It repeats to extract under conditions of 25kV/cm；
(2) merge extracting solution and filter, then in a rotary evaporator after concentration, be freeze-dried to get tartary buckwheat bran Beijing opera
Ketone extract.
Preferably, the ethanol solution is the aqueous solution of ethyl alcohol, volumetric concentration 70vol%.
Preferably, the number of repetition extraction is 2-4 times.
The preparation method of the composite preservative containing tartary buckwheat bran chromocor extract, includes the following steps：
(1) in terms of mass fraction, 10-25 parts of carboxymethyl chitosans is dissolved in 200 parts of aqueous acetic acids, are stirred overnight,
Obtain chitosan solution；Reprecipitation is carried out to carboxymethyl chitosan solution with sodium hydroxide solution again, is filtered, with distilled water and nothing
Water-ethanol washs filter cake repeatedly, until filtrate weakly acidic pH, vacuum drying, obtain carboxymethyl chitosan after purification；
(2) in terms of mass fraction, 10-25 parts of carboxymethyl chitosan, 0.1-0.5 parts of Tween-20s and water after purification is mixed
It closes, ultrasonic wave added dissolving obtains carboxymethyl chitosan mixed liquor；
(3) in terms of mass fraction, 30-40 parts of glycerine is mixed with 1-4 parts of tartary buckwheat bran chromocor extract, are obtained sweet
Oil-flavones mixed liquor；
(4) carboxymethyl chitosan mixed liquor is mixed with glycerine-flavones mixed liquor, it is multiple obtains coating-film fresh-keeping after mixing
With liquid；
(5) the coating-film fresh-keeping compositional liquor is placed in vacuum environment and is de-gassed, until bubble-free, obtains fresh-keeping
Agent.
Preferably, ultrasonic wave added dissolving instrument and equipment used is to be cleaned by ultrasonic instrument；Ultrasound is controlled in course of dissolution
Time 40-50min, ultrasonic power 300W, temperature are 45 DEG C -60 DEG C.
Preferably, the mass concentration of step (1) described carboxymethyl chitosan is 1%；The quality of the aqueous acetic acid is dense
Degree is 2%.
Preferably, a concentration of 1-2% of step (1) described sodium hydroxide solution.
The present invention also provides application of the antistaling agent in fresh agricultural products are fresh-keeping.
Composite preservative provided by the present invention obtains application method：Select it is fresh, have no mechanical damage, without rot and size
Uniform cherry or apple rinse surface with clean tap water, then naturally dry uses infusion method equal in apple surface
It is even to apply the prefabricated tartary buckwheat bran chromocor extract-carboxymethyl chitosan composite preservative of last layer, it is wrapped after natural air drying
Dress, you can play fresh-keeping effect.
Compared with the existing technology, beneficial effects of the present invention are：
(1) high-pressure pulse electric treatment technology belongs to non-heated processing, has and transmits uniform quick, processing time is short, production
Heat less, low energy consumption the advantages that, a variety of injuries that can overcome hot-working that may bring, the present invention application high-pressure pulse electric processing hardship
Buckwheat bran makes it that can be carried out under normal temperature condition, effectively increases the recovery rate of tartary buckwheat bran chromocor extract, and makes
It is high to obtain tartary buckwheat bran flavones purity.
(2) carboxymethyl chitosan is one kind of polysaccharide, and cross-linked structure can be by the main function object in chromocor extract
Matter rutin embeds, and then more preferably fungistatic effect, tartary buckwheat bran of the present invention are played on the basis of chitosan anti-bacteria fresh-keeping function
Skin chromocor extract and carboxymethyl chitosan are compound, and inoxidizability, broad-spectrum antibacterial activity significantly improve, and the composite preservative is green
Color is natural, nontoxic residue-free.
(3) there is chitosan anti-bacterial refreshing anti-corrosion ability, bitter buckwheat flavone extract to have good anti-oxidant in the present invention
Activity causes chain to lack mobility there are active force between chitosan molecule, and the film of formation is rigid structure and rough surface, with
Glycerine has softened the rigid structure of film as plasticizer, effectively improves the mechanical performance of film, is allowed to soft and high resilience；
In addition flavones can also improve the hydroscopicity of chitosan film, and good moisture-absorbing moisture-keeping performance can delay the transpiration of fruits and vegetables,
Reduce the moisture loss in storage；And Tween-20 is nonionic surfactant, primarily serves the work of dispersion and stabilization
With.The present invention not only improves composite preservative film by adding the coalescents such as qs glycerin in carboxymethyl chitosan
Homogeneity also enhances the integrality of its film forming, does not crack after so that the film is air-dried, and has good choke, moisture barrier property, has simultaneously
There are excellent anti-oxidant, fungistatic effect, and fresh-keeping effect is apparent.
(4) present invention improves the antioxygen of composite preservative by the addition of optimization tartary buckwheat bran chromocor extract
Change, antibacterial activity.
(5) each component of the present invention is environmentally protective (tartary buckwheat bran category refuse reclamation), good water solubility, and easy cleaning is nontoxic
Pair residual, has a vast market foreground.
Specific implementation mode
With reference to embodiment, the present invention will be further elaborated, it should which explanation, embodiment is merely to solution
The present invention is released, the scope of the present invention is not defined.
Ethanol solution used in the present invention is the aqueous solution of ethyl alcohol.
Embodiment 1
A kind of extraction of tartary buckwheat bran flavones, using following methods：
By dry tartary buckwheat bran and 70vol% ethanol solutions according to solid-liquid ratio 1：30 (g/mL) are mixed, and are uniformly mixed
Be placed on high-pressure pulse electric, circulation be 12mL/min, umber of pulse 10, electric field strength be 25kV/cm under conditions of repeat
Extraction is three times；Merge extracting solution and filter, then in a rotary evaporator after concentration, is freeze-dried to get tartary buckwheat bran flavones
Extract.
A kind of composite preservative containing tartary buckwheat bran chromocor extract, in terms of parts by weight, raw material group becomes：Bitter buckwheat
1 part of wheat bran skin chromocor extract, 10 parts of carboxymethyl chitosan, 30 parts of glycerine, 0.1 part of Tween-20,1000 parts of water.
A kind of preparation method of the composite preservative containing tartary buckwheat bran chromocor extract, includes the following steps：
(1) in terms of parts by weight, the carboxymethyl chitosan that 10 parts of mass concentrations are 1%, which is dissolved in 200 parts of mass concentrations, is
It in 2% aqueous acetic acid, is stirred overnight, obtains chitosan solution.The sodium hydroxide for being again 1% with 300 parts of mass concentrations is molten
Liquid carries out reprecipitation to carboxymethyl chitosan solution, filters, is washed repeatedly to filter cake with distilled water and absolute ethyl alcohol, until filtrate
Weakly acidic pH, vacuum drying, obtains carboxymethyl chitosan after purification.
(2) in terms of parts by weight, 10 parts of carboxymethyl chitosans, 0.1 part of Tween-20 are mixed with 1000 parts of water, ultrasound is auxiliary
Hydrotropy solution (ultrasonic time 40min, ultrasonic power 300W, temperature are 45 DEG C), obtains carboxymethyl chitosan mixed liquor.
(3) in terms of parts by weight, 30 parts of glycerine is mixed with 1 part of tartary buckwheat bran chromocor extract, obtain glycerine-flavones
Mixed liquor.
(4) carboxymethyl chitosan mixed liquor is mixed with glycerine-flavones mixed liquor, it is multiple obtains coating-film fresh-keeping after mixing
With liquid.
(5) above-mentioned fresh-keeping compositional liquid is placed in vacuum environment and is de-gassed, until bubble-free, obtains antistaling agent.
Embodiment 2
A kind of extraction of tartary buckwheat bran flavones, using following methods：
By dry tartary buckwheat bran and 70vol% ethanol solutions according to solid-liquid ratio 1：40 (g/mL) are mixed, and are uniformly mixed
Be placed on high-pressure pulse electric, circulation be 14mL/min, umber of pulse 12, electric field strength be 25kV/cm under conditions of repeat
Extraction is three times；Merge extracting solution and filter, then in a rotary evaporator after concentration, is freeze-dried to get tartary buckwheat bran flavones
Extract.
A kind of composite preservative containing tartary buckwheat bran chromocor extract, in terms of parts by weight, raw material group becomes：Bitter buckwheat
3 parts of wheat bran skin chromocor extract, 18 parts of carboxymethyl chitosan, 35 parts of glycerine, 0.25 part of Tween-20,1000 parts of water.
A kind of preparation method of the composite preservative containing tartary buckwheat bran chromocor extract, includes the following steps：(1) with
18 parts of mass concentrations are the aqueous acetic acid that 1% carboxymethyl chitosan is dissolved in that 200 parts of mass concentrations are 2% by parts by weight meter
In, it is stirred overnight, obtains chitosan solution.The sodium hydroxide solution for being again 1% with 300 parts of mass concentrations is to carboxymethyl chitosan
Solution carries out reprecipitation, filters, and distilled water and absolute ethyl alcohol wash filter cake repeatedly, until filtrate weakly acidic pH, vacuum drying, obtain
Carboxymethyl chitosan after purification.
(2) in terms of parts by weight, 18 parts of carboxymethyl chitosans, 0.25 part of Tween-20 are mixed with 1000 parts of water, ultrasound is auxiliary
Hydrotropy solution (ultrasonic time 40min, ultrasonic power 300W, 55 DEG C of temperature), obtains carboxymethyl chitosan mixed liquor.
(3) in terms of parts by weight, 35 parts of glycerine is mixed with 3 parts of tartary buckwheat bran chromocor extracts, obtain glycerine-flavones
Mixed liquor.
(4) carboxymethyl chitosan mixed liquor is mixed with glycerine-flavones mixed liquor, it is multiple obtains coating-film fresh-keeping after mixing
With liquid.
(5) above-mentioned fresh-keeping compositional liquid is placed in vacuum environment and is de-gassed, until bubble-free, obtains antistaling agent.
Embodiment 3
A kind of extraction of tartary buckwheat bran flavones, using following methods：
By dry tartary buckwheat bran and 70vol% ethanol solutions according to solid-liquid ratio 1：50 (g/mL) are mixed, and are uniformly mixed
Be placed on high-pressure pulse electric, circulation be 16mL/min, umber of pulse 14, electric field strength be 25kV/cm under conditions of repeat
Extraction 4 times；Merge extracting solution and filter, then in a rotary evaporator after concentration, freeze-drying carries to get tartary buckwheat bran flavones
Take object.
A kind of composite preservative containing tartary buckwheat bran chromocor extract, in terms of parts by weight, raw material group becomes：Bitter buckwheat
4 parts of wheat bran skin chromocor extract, 16 parts of carboxymethyl chitosan, 40 parts of glycerine, 0.5 part of Tween-20,1000 parts of water.
A kind of preparation method of the composite preservative containing tartary buckwheat bran chromocor extract, includes the following steps：
(1) it is that be dissolved in 200 parts of mass concentrations be 2% to 1% carboxymethyl chitosan by 16 parts of mass concentrations in terms of parts by weight
Aqueous acetic acid in, be stirred overnight, obtain chitosan solution.The sodium hydroxide solution pair for being again 1% with 300 parts of mass concentrations
Carboxymethyl chitosan solution carries out reprecipitation, filters, and distilled water and absolute ethyl alcohol wash filter cake repeatedly, until during filtrate is close
Property, vacuum drying obtains carboxymethyl chitosan after purification.
(2) in terms of parts by weight, 16 parts of carboxymethyl chitosans, 0.5 part of Tween-20 are mixed with 1000 parts of water, ultrasound is auxiliary
Hydrotropy solution (ultrasonic time 40min, ultrasonic power 300W, temperature 60 C), obtains carboxymethyl chitosan mixed liquor.
(3) in terms of parts by weight, 40 parts of glycerine is mixed with 4 parts of tartary buckwheat bran chromocor extracts, obtain glycerine-flavones
Mixed liquor.
(4) carboxymethyl chitosan mixed liquor is mixed with glycerine-flavones mixed liquor, it is multiple obtains coating-film fresh-keeping after mixing
With liquid.
(5) above-mentioned fresh-keeping compositional liquid is placed in vacuum environment and is de-gassed, until bubble-free, obtains antistaling agent.
Measure of merit：The fresh-keeping experiment of composite preservative prepared by embodiment 1-3 to apple.
Test method：Select 4 groups it is fresh, have no mechanical damage, without rotting and apple of uniform size, by embodiment 1-3 and
The antistaling agent of preparation is uniformly applied to the apple surface of 1-3 groups, and the 4th group is blank control group, natural without any processing
It air-dries, apple 30kg is handled per 1L composite preservatives, different time is stored at being 25 ± 2 DEG C in average room temperature, to its color, gas
Taste, form, hardness, weight-loss ratio and surface bacteria sum carry out effect assessment, and evaluation criterion is as follows：
Smell：There are the distinctive fragrance of apple, fresh and sweet oiliness A‐—A+；Apple aroma is light, or without apple aroma B‐—B+；
There are fermentation tart flavour or slight musty C‐—C+；Acid smell D‐—D+。
Color：Normal apple color, glossy A‐—A+；It is faint in color, tarnish B‐—B+；Apple surface spottiness
It bears, matt C‐—C+；There are a large amount of mildews to bear, and place pecky becomes yellowish-brown or dark brown D‐—D+。
Form：Institutional framework is good, full to have moisture A‐—A+；Institutional framework is preferable, the less full B of apple shape‐—B+；Institutional framework is poor, epidermal fold, no moisture C‐—C+；Institutional framework is poor, have it is a large amount of it is mould bear, the outflow of apple internal moisture
D‐—D+。
Weight-loss ratio is calculated using following formula：Quality x100% after quality/storage before weight-loss ratio=storage.
Surface bacteria sum uses colony counting method.It takes 5g (belt leather) to be placed on 100mL respectively 4 groups of above-mentioned apples to contain
5min is impregnated in the physiological saline of the Tween-80 of mass fraction 0.2%, soak is serially diluted (1:10) it, then adopts
With colony counting method, is cultivated in 37 DEG C of incubators for 24 hours, measure total number of bacteria.
Table 1 is the result that composite preservative prepared by embodiment 1-3 evaluates apple fresh-keeping with blank control group.Table 1 is opened up
5 indexs are shown, wherein the method that smell, color and form are all made of subjective appreciation carries out, and participates in the people of subjective appreciation every time
It is 25-30 people to count, and result is subjective appreciation statistical result in table；Weight-loss ratio calculates gained using formula, and total number of bacteria is using flat
Plate counting method.
Table 1 compared the influence of embodiment 1-3 and blank control group to apple physiology.Pass through embodiment 1-3 and blank pair
It does comparison according to the data of group and obtains：All have obviously than blank control group in the various aspects effect of the composite preservative of embodiment 1-3
Promotion, not only reached ideal fungistatic effect, but also do not interfere with the form of apple, the physics organoleptic properties such as color.It can be seen that
The compound preservative that invention tartary buckwheat bran chromocor extract is combined with carboxymethyl chitosan is imitated compared with blank control group fresh-keeping bacteriostatic
Fruit is apparent.Probably due to carboxymethyl chitosan is one kind of polysaccharide, cross-linked structure can be by the main work in chromocor extract
It is embedded with substance rutin, and then plays more preferably fungistatic effect, bitter buckwheat of the present invention on the basis of chitosan anti-bacteria fresh-keeping function
Wheat bran skin chromocor extract and carboxymethyl chitosan are compound, and inoxidizability, broad-spectrum antibacterial activity significantly improve, the composite fresh-keeping
Agent green natural, nontoxic residue-free.
Table 1
There is chitosan anti-bacterial refreshing anti-corrosion ability, bitter buckwheat flavone extract to have good anti-oxidant work in the present invention
Property, there are active forces between chitosan molecule, and chain is caused to lack mobility, and the film of formation is rigid structure and rough surface, with sweet
Oil is used as plasticizer, has softened the rigid structure of film, has effectively improved the mechanical performance of film, is allowed to soft and high resilience；Separately
Outer flavones can also improve the hydroscopicity of chitosan film, and good moisture-absorbing moisture-keeping performance can delay the transpiration of fruits and vegetables, subtract
Moisture loss in few storage；And Tween-20 is nonionic surfactant, primarily serves dispersion and the effect of stabilization.
It can be seen that there are coordinated effects for plurality of raw materials in composition of raw materials of the present invention.
The present invention improves the homogeneity of composite preservative film, enhances simultaneously by adding the coalescents such as qs glycerin
The integrality of film forming does not crack after so that film is air-dried, and not only has good choke, resistance moist, also have it is excellent it is anti-oxidant,
Fungistatic effect, and fresh-keeping effect is apparent.
Each component of the present invention is environmentally protective, good water solubility, easy cleaning, nontoxic secondary residual.
It should be noted that embodiment of the present invention are not limited by the above embodiments, it is other any without departing from this
The Spirit Essence of invention and the change made under principle, modification, combine, replacement, simplification should be equivalence replacement mode, all include
Within protection scope of the present invention.
Claims (9)
1. a kind of composite preservative containing tartary buckwheat bran chromocor extract, which is characterized in that by following raw material group
At：1-4 parts of tartary buckwheat bran chromocor extract, 10-25 parts of carboxymethyl chitosan, 30-40 parts of glycerine, Tween-20 0.1-0.5
Part, 1000 parts of water.
2. the composite preservative according to claim 1 containing tartary buckwheat bran chromocor extract, which is characterized in that described
Tartary buckwheat bran chromocor extract is made using following methods：
(1) respectively as unit of g and mL, by dry tartary buckwheat bran and ethanol solution according to solid-liquid ratio 1：(30-50) is mixed,
It is uniformly mixed and is placed on high-pressure pulse electric, be 12-16mL/min, umber of pulse 10-14, electric field strength 25kV/ in circulation
It repeats to extract under conditions of cm；
(2) merge extracting solution and filter, then in a rotary evaporator after concentration, freeze-drying carries to get tartary buckwheat bran flavones
Take object.
3. the composite preservative according to claim 2 containing tartary buckwheat bran chromocor extract, it is characterised in that：It is described
Ethanol solution be ethyl alcohol aqueous solution, volumetric concentration 70vol%.
4. the composite preservative according to claim 2 containing tartary buckwheat bran chromocor extract, it is characterised in that：It is described
Repetition extraction number be 2-4 times.
5. claim 1-4 any one of them contains the preparation method of the composite preservative of tartary buckwheat bran chromocor extract,
It is characterized by comprising the following steps：
(1) in terms of mass fraction, 10-25 parts of carboxymethyl chitosans is dissolved in 200 parts of aqueous acetic acids, is stirred overnight, obtains
Chitosan solution；Reprecipitation is carried out to carboxymethyl chitosan solution with sodium hydroxide solution again, is filtered, with distilled water and anhydrous second
Alcohol washs filter cake repeatedly, until filtrate weakly acidic pH, vacuum drying, obtain carboxymethyl chitosan after purification；
(2) in terms of mass fraction, 10-25 parts of carboxymethyl chitosan, 0.1-0.5 parts of Tween-20s after purification are mixed with water, is surpassed
Sound auxiliary dissolving, obtains carboxymethyl chitosan mixed liquor；
(3) in terms of mass fraction, 30-40 parts of glycerine is mixed with 1-4 parts of tartary buckwheat bran chromocor extract, obtain glycerine-
Flavones mixed liquor；
(4) carboxymethyl chitosan mixed liquor is mixed with glycerine-flavones mixed liquor, obtains coating-film fresh-keeping compounding after mixing
Liquid；
(5) the coating-film fresh-keeping compositional liquor is placed in vacuum environment and is de-gassed, until bubble-free, obtains antistaling agent.
6. the preparation method of the composite preservative according to claim 5 containing tartary buckwheat bran chromocor extract, special
Sign is that ultrasonic wave added dissolving instrument and equipment used is to be cleaned by ultrasonic instrument；Ultrasonic time 40- is controlled in course of dissolution
50min, ultrasonic power 300W, temperature are 45 DEG C -60 DEG C.
7. the preparation method of the composite preservative according to claim 5 containing tartary buckwheat bran chromocor extract, special
Sign is that the mass concentration of step (1) described carboxymethyl chitosan is 1%；The mass concentration of the aqueous acetic acid is 2%.
8. the preparation method of the composite preservative according to claim 5 containing tartary buckwheat bran chromocor extract, special
Sign is, a concentration of 1-2% of step (1) described sodium hydroxide solution.
9. a kind of application of composite coating preservative according to any one of claims 1-4 in fresh agricultural products are fresh-keeping.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074173A (en) * | 2019-05-24 | 2019-08-02 | 陕西师范大学 | A kind of goat milk and mutton antistaling agent |
| CN112425711A (en) * | 2020-11-23 | 2021-03-02 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
| CN114158611A (en) * | 2021-12-20 | 2022-03-11 | 南京泛成生物科技有限公司 | Preparation and application of carboxymethyl chitosan-based preservative film |
| CN115612131A (en) * | 2022-09-07 | 2023-01-17 | 长江师范学院 | Method for preparing antibacterial preservative film by using longan shell extract and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105282A (en) * | 2015-09-14 | 2015-12-02 | 青岛农业大学 | Composite coating fresh keeping agent containing hawthorn leaf flavone and preparation method and application thereof |
| CN105285077A (en) * | 2015-09-14 | 2016-02-03 | 青岛农业大学 | Composite coating film fresh keeping agent containing persimmon leaf flavones as well as preparation method and application of composite coating film fresh keeping agent |
| CN105998139A (en) * | 2016-07-05 | 2016-10-12 | 华宝食用香精香料（上海）有限公司 | Method for extracting flavone from sweet potato leaves |
| CN107372770A (en) * | 2017-06-20 | 2017-11-24 | 兰溪市捷喜食品加工技术有限公司 | Antistaling agent based on shell and preparation method thereof |
-
2018
- 2018-03-28 CN CN201810263238.1A patent/CN108497057A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105105282A (en) * | 2015-09-14 | 2015-12-02 | 青岛农业大学 | Composite coating fresh keeping agent containing hawthorn leaf flavone and preparation method and application thereof |
| CN105285077A (en) * | 2015-09-14 | 2016-02-03 | 青岛农业大学 | Composite coating film fresh keeping agent containing persimmon leaf flavones as well as preparation method and application of composite coating film fresh keeping agent |
| CN105998139A (en) * | 2016-07-05 | 2016-10-12 | 华宝食用香精香料（上海）有限公司 | Method for extracting flavone from sweet potato leaves |
| CN107372770A (en) * | 2017-06-20 | 2017-11-24 | 兰溪市捷喜食品加工技术有限公司 | Antistaling agent based on shell and preparation method thereof |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110074173A (en) * | 2019-05-24 | 2019-08-02 | 陕西师范大学 | A kind of goat milk and mutton antistaling agent |
| CN110074173B (en) * | 2019-05-24 | 2022-08-30 | 陕西师范大学 | Goat milk and mutton preservative |
| CN112425711A (en) * | 2020-11-23 | 2021-03-02 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
| CN112425711B (en) * | 2020-11-23 | 2023-01-24 | 东北林业大学 | Acorn shell extract coating preservative and preparation method thereof |
| CN114158611A (en) * | 2021-12-20 | 2022-03-11 | 南京泛成生物科技有限公司 | Preparation and application of carboxymethyl chitosan-based preservative film |
| CN114158611B (en) * | 2021-12-20 | 2023-08-11 | 南京泛成生物科技有限公司 | Preparation and application of carboxymethyl chitosan-based preservative film |
| CN115612131A (en) * | 2022-09-07 | 2023-01-17 | 长江师范学院 | Method for preparing antibacterial preservative film by using longan shell extract and application |
| CN115612131B (en) * | 2022-09-07 | 2024-02-20 | 长江师范学院 | Method for preparing antibacterial preservative film by using longan shell extract and application of antibacterial preservative film |
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