CN108490007B - Method for distinguishing artemisia rupestris L and artemisia rupestris L charcoal by adopting X-ray diffraction method - Google Patents
Method for distinguishing artemisia rupestris L and artemisia rupestris L charcoal by adopting X-ray diffraction method Download PDFInfo
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- CN108490007B CN108490007B CN201810565164.7A CN201810565164A CN108490007B CN 108490007 B CN108490007 B CN 108490007B CN 201810565164 A CN201810565164 A CN 201810565164A CN 108490007 B CN108490007 B CN 108490007B
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Abstract
The invention provides a method for distinguishing artemisia anomala from artemisia anomala charcoal by an X-ray diffraction method, which is characterized by identifying by an X-ray diffraction peak. The research of the invention finds that by adopting an X-ray diffraction method, the artemisia anomala and the artemisia anomala charcoal can be distinguished through characteristic peaks, the difference between the two can be found, and the help is provided for distinguishing and identifying the two.
Description
Technical Field
The invention belongs to the technical field of medicinal material distinguishing and identifying.
Background
Charcoal medicinal materials are widely applied clinically and have a long history. The Pueraria scandens (artemisia anomala) is one of the common herbal medicines in Tibetan medicine, is an important medicine mainly used for treating various inflammatory manifestations in Tibetan medicine, can be directly ground into powder, decocted into paste or calcined into ash (calcined and roasted), and is commonly used in the formula: it is often used to clear heat and remove toxicity, diminish inflammation and plague by decocting or directly taking it as medicine; calcined ash is commonly used for treating diseases such as diseases, sores and furuncles, hemostasis, dry pus or yellow water, etc. According to literature records, when the artemisia rupestris L.is calcined into charcoal, the medicinal materials are placed in an earthenware pot or an iron pot, a cover is covered, a seam is tightly sealed by salt slurry, the medicine is calcined by medium fire, the medicine is tightly calcined until the medicine is completely calcined, the medicine is cooled after being separated from the fire, and the medicine is taken out and ground into powder.
However, the chemical composition of the artemisia rupestris L after the charring is less studied. At present, a method for distinguishing the artemisia rupestris L from the artemisia rupestris L charcoal is not seen.
Disclosure of Invention
X-ray diffraction analysis (X-ray diffraction analysis) is an effective spectrum analysis method capable of reflecting the overall characteristics of the molecular structure of each chemical component contained in the inherent form of a traditional Chinese medicine without intervention of chemical solvents. The plant medicine is composed of multiple chemical components, wherein amorphous substances (such as polysaccharide, starch, cellulose, protein and the like) are dispersed in a diffraction pattern to form the geometric topological characteristic of the diffraction pattern, and crystalline substances (such as calcium oxalate crystals and other components) are superposed on the diffraction pattern by a sharp special diffraction spectral line to form a special pattern with fingerprint and graphic topology. The invention aims to adopt an X-ray diffraction analysis method to correspondingly analyze the artemisia anomala and the artemisia anomala charcoal samples and find a method for distinguishing and identifying the two by X-ray diffraction.
The method for distinguishing the artemisia anomala from the artemisia anomala charcoal by the X-ray diffraction method is characterized in that dry medicinal material powder of a sample to be detected of the artemisia anomala or the artemisia anomala charcoal is taken, and the detection is carried out by the X-ray diffraction method under Co target Ka radiation; when the 2 theta angle has diffraction peaks at about 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 42.64, 46.38, 47.44, 48.10 and 52.24, the sample to be detected is artemisia rupestris; when the angle 2 theta has diffraction peaks at 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 30.22, 31.44, 31.84, 35.36, 37.22, 39.40, 40.62, 41.46, 50.30 and 58.76, the sample to be detected is artemisia rupestris L.
Further, when the 2 theta angle has diffraction peaks at about 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 29.98, 38.08, 42.64, 42.98, 44.34, 46.38, 47.44, 48.10, 52.24, 54.68 and 57.00, the sample to be detected is artemisia anomala; when the 2 theta angle has diffraction peaks at about 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 29.76, 30.22, 31.44, 31.84, 35.36, 37.22, 37.98, 39.40, 40.62, 41.46, 43.28, 44.16, 44.60, 50.30, 55.04, 57.88 and 58.76, the sample to be detected is artemisia anomala charcoal.
Furthermore, when the X-ray diffraction spectrum is shown in figure 1, the sample to be detected is artemisia rupestris; when the X-ray diffraction spectrum is shown in figure 2, the sample to be detected is artemisia anomala charcoal.
The invention also provides a method for identifying the artemisia anomala by adopting an X-ray diffraction method, which comprises the steps of taking dry medicinal material powder of a sample to be detected, and detecting the dry medicinal material powder under Co target Ka radiation by adopting the X-ray diffraction method; when the 2 theta angle has diffraction peaks at about 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 42.64, 46.38, 47.44, 48.10 and 52.24, the sample to be detected is artemisia rupestris.
Further, when the 2 theta angle has diffraction peaks at about 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 29.98, 38.08, 42.64, 42.98, 44.34, 46.38, 47.44, 48.10, 52.24, 54.68 and 57.00, the sample to be detected is artemisia anomala.
The invention also provides a method for identifying the artemisia anomala charcoal by adopting an X-ray diffraction method, which comprises the steps of taking dry medicinal material powder of a sample to be detected, and detecting under Co target Ka radiation by adopting the X-ray diffraction method; when the angle 2 theta has diffraction peaks at 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 30.22, 31.44, 31.84, 35.36, 37.22, 39.40, 40.62, 41.46, 50.30 and 58.76, the sample to be detected is artemisia rupestris L.
Further, when the 2 theta angle has diffraction peaks at about 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 29.76, 30.22, 31.44, 31.84, 35.36, 37.22, 37.98, 39.40, 40.62, 41.46, 43.28, 44.16, 44.60, 50.30, 55.04, 57.88 and 58.76, the sample to be detected is artemisia anomala charcoal.
The research of the invention finds that by adopting an X-ray diffraction method, the artemisia anomala and the artemisia anomala charcoal can be distinguished through characteristic peaks, the difference between the two can be found, and the help is provided for distinguishing and identifying the two.
Drawings
FIG. 1X-ray diffraction Fourier diagram of Artemisia rupestris
FIG. 2X-ray diffraction Fourier diagram of mugwort charcoal
Detailed Description
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Instrument for measuring the position of a moving object
Pujingyo XD-6X-ray powder diffractometer (Beijing Pujingyo general instruments, LLC); ESJ200-4 electronic balance (Shenyang Longteng electronics Co., Ltd.); METTLER AE240 electronic analytical balance [ Mettler-Torledo (Shanghai) instruments, Inc. ]; HX-200 type high-speed Chinese medicine grinder (Zhejiang Yongkang Xi Chi hardware medical and medical apparatus factory).
Materials and reagents
Artemisia rupestris and Artemisia rupestris charcoal were purchased from Qiang autonomous State Tibetan Hospital in Abam, Sichuan province at 7 months and 9 days in 2014 (see Table 1).
TABLE 1 Artemisia ordosica (charred) samples
Example 1
1. Sample source and processing
And (3) drying the sample at 60 ℃ for 6h, crushing, and sieving with a 100-mesh sieve for later use.
2. X-ray diffraction test
The test conditions are as follows: co target Ka radiation; the scanning mode is as follows: qualitative and step scanning; voltage/current: 36kV/20mA, step width: 0.02 degree; scanning range: 3 to 60 degrees.
3. Results and analysis
The experimental data were treated with originPro 8.5 (35 points smoothed) to give a powder diffraction pattern, see FIGS. 1 and 2. The data were processed with Jade6.0 to find peaks at 2 θ, interplanar spacing d and relative intensity I/IoIs expressed as 2 theta, d (I/I)o)。
FIG. 1 shows an X-ray diffraction Fourier spectrum of Artemisia rupestris, and the corresponding 19 diffraction characteristic peaks are: 6.00, 14.72(41.0), 6.84, 12.91(39.6), 10.28, 8.60(68.1), 10.82, 8.17(55.6), 16.82, 5.27(40.7), 17.32, 5.12(43.9), 21.52, 4.13(79.8), 29.50, 3.03(37.9), 29.98, 2.98(37.0), 38.08, 2.36(100.0), 42.64, 2.12(24.2), 42.98, 2.10(27.6), 44.34, 2.04(88.6), 46.38, 1.96(24.5), 47.44, 1.91(23.4), 48.10, 1.89(22.8), 52.24, 1.75(23.1), 54.68, 1.68(17.9), 17.00, 1.4 (17.9).
FIG. 2 shows an X-ray diffraction Fourier spectrum of Artemisia rupestris L charcoal, wherein the corresponding 24 diffraction characteristic peaks are as follows: 10.40, 8.50(17.8), 12.06, 7.33(16.7), 14.10, 6.28(16.2), 18.30, 4.84(13.1), 24.38, 3.65(25.1), 25.30, 3.52(12.5), 28.50, 3.13(100.0), 29.76, 3.00(13.5), 30.22, 2.95(16.4), 31.44, 2.84(27.5), 31.84, 2.81(11.8), 35.36, 2.54(10.1), 37.22, 2.41(8.8), 37.98, 2.37(9.4), 39.40, 2.28(9.8), 40.62, 2.22(64.4), 41.46, 2.18(9.1), 26, 2.09(9.7), 16.44, 2.7), 3.7), 14.9.7, 3.9.9.9.9.9, 1, 3.9.7, 3.9.9.1, 3.9.7, 3.9.9.9.7, 3.9.1, 3.9.9.9.7, 3.9.9.1, 3.9.9.9.9.1, 35.7, 35.9.9.9.9.9.
4 small knot
The results of X-diffraction analysis show that the chemical components of the artemisia anomala after being subjected to hard-cover calcination to form charcoal change obviously, diffraction peaks in an XRD (X-ray diffraction) spectrum have obvious difference, and the artemisia anomala charcoal can be effectively distinguished and identified according to the XRD diffraction peaks.
Claims (2)
1. The method for distinguishing the artemisia rupestris L and the artemisia rupestris L charcoal by adopting the X-ray diffraction method is characterized by comprising the following steps of: taking dry medicinal material powder of the sample to be detected of the artemisia rupestris or artemisia rupestris charcoal, and detecting the dry medicinal material powder under Co target Ka radiation by adopting an X-ray diffraction method; when the 2 theta angle has diffraction peaks at 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 42.64, 46.38, 47.44, 48.10 and 52.24, the sample to be detected is artemisia rupestris; when the angle 2 theta has diffraction peaks at 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 30.22, 31.44, 31.84, 35.36, 37.22, 39.40, 40.62, 41.46, 50.30 and 58.76, the sample to be detected is artemisia rupestris L.
2. The method of claim 1, wherein: when the 2 theta angle has diffraction peaks at 6.00, 6.84, 10.28, 10.82, 16.82, 17.32, 21.52, 29.50, 29.98, 38.08, 42.64, 42.98, 44.34, 46.38, 47.44, 48.10, 52.24, 54.68 and 57.00, the sample to be detected is artemisia rupestris; when the 2 theta angle has diffraction peaks at 10.40, 12.06, 14.10, 18.30, 24.38, 25.30, 28.50, 29.76, 30.22, 31.44, 31.84, 35.36, 37.22, 37.98, 39.40, 40.62, 41.46, 43.28, 44.16, 44.60, 50.30, 55.04, 57.88 and 58.76, the sample to be detected is artemisia anomala charcoal.
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| CN103145661A (en) * | 2013-03-25 | 2013-06-12 | 成都天台山制药有限公司 | New crystal form of andrographolide |
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