CN108489967A - A kind of micro- spectral method traced to the source for phoenix tail chicken - Google Patents
A kind of micro- spectral method traced to the source for phoenix tail chicken Download PDFInfo
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Abstract
The present invention relates to a kind of micro- spectral methods traced to the source for phoenix tail chicken, belong to phoenix tail chicken breed, place of production tracing technology field.The present invention is by measuring type and content micro- in phoenix tail chicken and white feather chicken, then Multiple range test analysis is carried out, obtaining the content in phoenix tail chicken and white feather chicken, there are the micro- types of significant difference, then it obtains differentiating effective variable to kind by principal component analysis, hierarchical clustering method analysis and discriminant analysis, establish discrimination model, and the validity of model is verified, it thus obtains that there is effective taste to phoenix tail chicken sample, can be applied to the Trace Elements that phoenix tail chicken is traced to the source.This method can determine the Trace Elements that can be used in phoenix tail chicken breed, the place of production is traced to the source, the content of three kinds of elements in discriminant analysis model and sample can be passed through, accurately, phoenix tail chicken is quickly and to effectively identified, can be advantageously applied in the tracing to the source and differentiate of phoenix tail chicken.
Description
Technical field
The present invention relates to a kind of methods that species are traced to the source, it is more particularly related to which a kind of trace to the source for phoenix tail chicken
Micro- spectral method, belong to phoenix tail chicken breed, place of production tracing technology field.
Background technology
Phoenix tail chicken is the one kind for hiding chicken, has very strong ability of looking for food and circle in the air, fit for depasturing free-ranging is Sichuan Province's Aba
The distinguishing products of state Heishui County, the special ecological environment in plateau have bred abundant and unique species resource.Heishui County belongs to half agriculture
The counties Ban Mu, the high mountain gorge area in the middle part of west Sichuan plateau, Abazangzuqiangzu Autonomous Prefecture, are located in Mount Min mountain range stage casing, are
The cradle of Virgin Forest fork Babian Jiang and Mao Er lids river, geomorphic type are with plateau, high mountain gorge and middle mountains and rivers paddy
It is main, it is 1790-5286 meters above sea level.Special humanity and geographical environment has continued the traditional breeding way of Heisui River phoenix tail chicken.Phoenix tail chicken
It is grown in the Heishui County of plateau climate, whole year is raised in a manner of the Continous grazing of grassland, what special growing environment was cultivated,
2014, Heisui River phoenix tail chicken was cited as national agricultural product geography symbol product.Therefore, evaluation phoenix tail chicken quality, research and development phoenix tail chicken
Tracing technology is shaken off poverty and set out on the road to prosperity and is had for exploitation local varieties, raising Additional Value of Commodities, the local aquaculture of promotion Heishui County development
Significance.
With reference to existing research, to the classification of " chicken ", there are mainly two types of methods：First, carrying out microcosmic point according to genetic marker
Class, second is that carrying out macroscopical classification according to producing region distribution, form, body ruler and ecological characteristic.
There is unique ecological environment, mountain stream to trickle natural, limpid stream and thawing for the Heishui County of phoenix tail chicken growth
Snow-broth, phoenix tail chicken has very strong ability of looking for food and circle in the air, unique to herd foster mode its food is made to all originate from nature ring
In border, and the close phase such as the habit and ecological environment of each nutrition composition in phoenix tail chicken and it, i.e. soil, insect, plant, season
It closes, and because of the difference of life style and predation between species, creates unique trophic component structure between species.
But there is presently no one accurately and effectively standard kind quickly is carried out to phoenix tail chicken and the open place of production is traced to the source.
State Intellectual Property Office discloses a Publication No. CN106770617A, a kind of entitled " profit in 2017.5.31
With trace element and rare earth element assay combination multi-variate statistical analysis the method that the place of production is traced to the source is carried out to Radix Salviae Miltiorrhizae " invention,
The disclosure of the invention it is a kind of using trace element and rare earth element assay combination multi-variate statistical analysis to the Radix Salviae Miltiorrhizae progress place of production
The method traced to the source, this method have carried out difference analysis using one-way analysis of variance to all elements of all samples.Work as p<
When 0.05, it is believed that the element data of different producing area has notable difference.Principal component analysis is analyzed for Data Dimensionality Reduction, linear discriminant point
For assessing different producing area red rooted salvia, whether there is or not significant differences for analysis.A kind of method traced to the source the Radix Salviae Miltiorrhizae progress place of production is provided, it should
Method has the advantages that sensitive, quick, differentiation accuracy is high.
State Intellectual Property Office discloses a Publication No. CN105259160A, a kind of entitled " base in 2016.1.20
In the West Lake Dragon Well tea Production area recognition method of ionomics " invention, a kind of West Lake based on ionomics of the disclosure of the invention
Longjing tea Production area recognition method, it is characterised in that include the following steps：1) tealeaves of the selection from the West Lake Dragon Well tea place of production represents
Property sample；2) sample pretreatment；3) sample analysis measures；4) the Production area recognition technology model of West Lake Dragon Well tea is built；It 5) will not
The Production area recognition technology model for knowing the Tea Samples data input tealeaves of place of production information, predicts tea-leaf producing area information.It should
Method can not only meet the quality safety of tealeaves, also can effectively contain the personation of tealeaves, also effectively can recall or cancel and ask
The tea products of topic are correctly sentenced to farthest reduce economic loss using the model acquired in Partial Least Squares
Rate is not 94.4%, and predictablity rate 92.0%, model is reliable.
This two pieces invention disclose it is a kind of using trace element and rare earth element assay combination multi-variate statistical analysis
The method that the place of production is traced to the source is carried out to plant, this method has carried out difference using one-way analysis of variance to all elements of all samples
Specific analysis.Work as p<When 0.05, it is believed that the element data of different producing area has notable difference.Principal component analysis is for Data Dimensionality Reduction point
Analysis, linear discriminant analysis be respectively used to assessment different producing area red rooted salvia and tealeaves whether there is or not significant differences.It is a kind of right to provide
Plant carries out the method that the place of production is traced to the source, and this method has the advantages that sensitive, quick, differentiation accuracy is high.
Though trace element, which is traced to the source, has applied to tracing to the source in plant, the purpose of " tracing to the source " all needs in a variety of distinguishing products
It wants.The quality of foodstuff product mainly determines by its component, and big group it is sub-category include carbohydrate, protein, lipid,
Inorganic salts, vitamin, water, and each major class includes various classifications and component, trace element is only one in inorganic salts
There is characteristic to need, by measuring and research just can determine that, can not directly push away for class, which constituents in a certain food
It is disconnected；Two kinds of plants of Radix Salviae Miltiorrhizae and tealeaves, clearly different plants have Specific adsorption to different heavy metals for research both at home and abroad at present
Effect；But animal be by ingest (including plant and animal) obtain nutrition, and food is needed in vivo by digesting and assimilating
The part in tissue could be become, it is completely different that this, which is shifted to new management mechanisms in animals and plants, so micro- in plant
Content and ratio can't retain in the animal body for taking in the food, therefore Trace Elements Features can not push away from plant
It measures in animal tissue.
Trace element in plant is advanced due to food chain in the soil of growth, in growing environment, habit
Under the influence of predation, micro- difference becomes more notable between animal species, and chicken lacks always the parameter of system,
The micronutrient levels data that the actual value of region can such as be represented, also lack the global analysis to Trace Elements Features, especially lack
The area of weary Trace Elements and species difference analysis.The above method can carry out the place of production to plant and trace to the source, but can not needle
To phoenix tail chicken, it is determined to the standard traced to the source for its kind, the place of production.
Invention content
Present invention seek to address that method in the prior art can not be directed to phoenix tail chicken, it is determined to for its kind, the place of production
The problem of standard traced to the source, provides a kind of micro- spectral method traced to the source for phoenix tail chicken, and this method can be determined can
For the Trace Elements that phoenix tail chicken breed, the place of production are traced to the source, with it is accurate, quickly and to effectively identify phoenix tail chicken.
In order to achieve the above-mentioned object of the invention, specific technical solution is as follows：
A kind of micro- spectral method traced to the source for phoenix tail chicken, it is characterised in that：Including following methods step：
A, phoenix tail chicken chicken meat sample and white feather chicken chicken meat sample are taken；
B, phoenix tail chicken chicken meat sample and the element species in white feather chicken chicken meat sample and content are measured；
C, the type and content of two kinds of chicken trace elements are obtained by step B, then carries out Multiple range test analysis, obtains two
Content in kind chicken tentatively infers that the trace element of these significant differences can there are the micro- type of significant difference
Applied to tracing to the source for phoenix tail chicken；
D, using Principal Component Analysis and hierarchical clustering method to tentatively infer in step C can be applied to phoenix tail chicken trace to the source it is micro-
Secondary element is further analyzed, and verifying these types of trace element can distinguish phoenix tail chicken with white feather chicken；
E, discriminant analysis is carried out, is selected from several trace elements with significant difference and effective become is differentiated to kind
Amount, establishes discrimination model, and verify the validity of model, thus obtains having effective taste to phoenix tail chicken sample, can apply
In the Trace Elements that phoenix tail chicken is traced to the source.
It is currently preferred, in step, specially：Take 6 phoenix tail chicken samples and 6 white feather chicken samples；Wherein phoenix tail
Chicken sample is by 3 phoenix tail chicken chicken leg samples and 3 phoenix tail chicken pigeon breast sample compositions；Wherein white feather chicken sample is by 3 white feather chicken chickens
Leg sample and 3 white feather chicken pigeon breast sample compositions.
It is currently preferred, in stepb, specially：Using micro-wave digestion inductivity coupled plasma mass spectrometry (ICP-
MS it) is measured in phoenix tail chicken and white feather chicken with micro-wave digestion inductively coupled plasma atomic emission (ICP-OES) two methods
Element species and content, wherein using biochemical component-chicken as internal standard substance.
Currently preferred, in step C, there are the micro- types of significant difference for the content in described two chicken
For Mn, Sr, Mo, Cs and Ag.
It is currently preferred, in step E, is selected from several trace elements with significant difference and kind is differentiated
Effective variable is Mn, Cs and Ag.
It is currently preferred, in step E, establish discrimination model in validation test using 2/3 sample as test set, 1/3
Sample combines the validity of cross-validation method verification model as extrapolation verification collection.
Currently preferred, in step E, the discrimination model is as follows：
Model 1 (A)=- 86.261+0.199Mn+0.370Ag-0.552Cs
Model 2 (B)=- 15.283+0.059Mn+0.367Cs+0.088Ag
Model 3 (C)=- 20.251+0.017Mn+0.995Cs-0.005Ag
Model 4 (D)=- 36.722-0.018Mn+1.589Cs-0.062Ag
Wherein model 1-4 indicates phoenix tail chicken chicken leg sample, phoenix tail chicken pigeon breast sample, white feather chicken chicken leg sample and Bai Yu respectively
Chicken pigeon breast sample.
The advantageous effects that the present invention is brought：
1, the present invention, which solves method in the prior art, can not be directed to phoenix tail chicken, be determined to for its kind, the place of production
The problem of standard traced to the source, provides a kind of micro- spectral method traced to the source for phoenix tail chicken, and this method can be determined can
For the Trace Elements that phoenix tail chicken breed, the place of production are traced to the source, can be contained by three kinds of elements in discriminant analysis model and sample
Amount, it is accurate, quickly and to effectively identify phoenix tail chicken, it can be advantageously applied in the tracing to the source and differentiate of phoenix tail chicken.
2, currently preferred, in step, specially：Take 6 phoenix tail chicken samples and 6 white feather chicken samples；Wherein phoenix
Tail chicken sample is by 3 phoenix tail chicken chicken leg samples and 3 phoenix tail chicken pigeon breast sample compositions；Wherein white feather chicken sample is by 3 white feather chickens
Chicken leg sample and 3 white feather chicken pigeon breast sample compositions.It is analyzed above by the sample of two breeder different parts, it can be more
Accurately determine out the Trace Elements traced to the source for phoenix tail chicken breed, the place of production.
3, currently preferred, in stepb, specially：Using micro-wave digestion inductivity coupled plasma mass spectrometry (ICP-
MS it) is measured in phoenix tail chicken and white feather chicken with micro-wave digestion inductively coupled plasma atomic emission (ICP-OES) two methods
Element species and content, wherein using biochemical component-chicken as internal standard substance.Two kinds of assay methods are combined, are avoided micro-
In addition the missing inspection of secondary element uses biochemical component-chicken as internal standard substance, effectively corrects the interference of matrix.
4, currently preferred, it is proposed that specific 4 discriminant analysis models correspond to phoenix tail chicken chicken leg sample, phoenix respectively
Tail chicken pigeon breast sample, white feather chicken chicken leg sample and white feather chicken pigeon breast sample.The group model is applicable to phoenix tail chicken breed, the place of production
It traces to the source, after Mn, Cs and Ag content for detecting sample and reference substance (white feather chicken), substitutes into model and pass through statistical analysis software
Calculate analysis, it will be able to rapidly and accurately judge whether sample is phoenix tail chicken.
Description of the drawings
Fig. 1 is the chart of principal component analysis in the embodiment of the present invention 3, and figure the left side of the table is phoenix tail chicken, and right side is white feather chicken；
Fig. 2 is the chart that hierarchical clustering method is analyzed in the embodiment of the present invention 3.
Specific implementation mode
Embodiment 1
A kind of micro- spectral method traced to the source for phoenix tail chicken, including following methods step：
A, phoenix tail chicken chicken meat sample and white feather chicken chicken meat sample are taken；
B, phoenix tail chicken chicken meat sample and the element species in white feather chicken chicken meat sample and content are measured；
C, the type and content of two kinds of chicken trace elements are obtained by step B, then carries out Multiple range test analysis, obtains two
Content in kind chicken tentatively infers that the trace element of these significant differences can there are the micro- type of significant difference
Applied to tracing to the source for phoenix tail chicken；
D, using Principal Component Analysis and hierarchical clustering method to tentatively infer in step C can be applied to phoenix tail chicken trace to the source it is micro-
Secondary element is further analyzed, and verifying these types of trace element can distinguish phoenix tail chicken with white feather chicken；
E, discriminant analysis is carried out, is selected from several trace elements with significant difference and effective become is differentiated to kind
Amount, establishes discrimination model, and verify the validity of model, thus obtains having effective taste to phoenix tail chicken sample, can apply
In the Trace Elements that phoenix tail chicken is traced to the source.
Amino acid, carbohydrate, micro member have all been carried out to phoenix tail chicken and the presently commercially available most wide white feather chicken present invention
The many indexes such as element detect, and there were significant differences for 5 kinds of discovery in the 15 kinds of trace elements detected, continues through statistics credit
It analyses (principal component analysis, clustering, discriminant analysis, verification, modeling), just finds that wherein three kinds have specificity, and by this
100% ground of phoenix tail chicken can coincide by the model of invention to be identified.This Research on Statistics and Analysis process needs are practised to animal
Property, source, known to local habitat on the basis of, the detection and data analysis of Multiple components are carried out, to find internal rule.
Embodiment 2
One, amino acid classes and sample explanation are detected
Detect aspartic acid, threonine, serine, glutamic acid, proline, glycine, alanine, Guang ammonia in phoenix tail chicken
18 kinds of acid, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, histidine, lysine, arginine ammonia
The content of base acid, and each amino acid classes and content of itself and commercially available white feather chicken are compared and analyzed.Sample illustrate as
Under：
Phoenix tail chicken chicken leg meat is respectively labeled as：S1、S2、S3；Phoenix tail chicken Fresh Grade Breast is respectively labeled as S4, S5, S6；Totally 6
Sample；White feather chicken chicken leg meat is respectively labeled as：C1、C2、C3；Commercially available white feather chicken Fresh Grade Breast is respectively labeled as C4, C5, C6；Totally 6
Sample, two kinds of kind chickens amount to 12 samples.
Two, detection method and data analysis reference standard
Amino acid content detection method is according to GB/T5009.124-2016 in phoenix tail chicken《National food safety standard food
The measurement of middle amino acid》.
Three, detecting instrument
The full-automatic amino-acid analyzers of Britain phase promise (Biochrom) 30+
Four, different parts muscle amino acid total amount
As shown in Table 1, total amino acid content is lower than Fresh Grade Breast in phoenix tail chicken chicken leg meat, respectively 22.55g/100g,
26.39g/100g the case where this is with other chickens are almost the same.The white feather chicken of detection is also in this way, respectively 21.51g/ simultaneously
100g、24.55g/100g.Therefore, the total amino acid content of chicken-breasted is usually higher than das Beinfleisch.
In chicken leg, serine and arginine content are all remarkably higher than white feather chicken (p in phoenix tail chicken<0.05).In Fresh Grade Breast
In, phoenix tail chicken Glutamic Acid, glycine and alanine content are all remarkably higher than white feather chicken (p<0.05).Although in pigeon breast and chicken leg
In meat, total amino acid content is slightly above white feather chicken in phoenix tail chicken, but does not have significant difference, it is believed that almost the same.
Table 1：Different chicken kind different parts Amino Acid Contents of Muscle and relative amount
Note：* the significant difference (p of amino acid content of phoenix tail chicken and white feather chicken is indicated<0.05)
Five, phoenix tail chicken muscle amino acid forms
The various amino acid relative amounts of phoenix tail chicken have a certain difference.As shown in Table 1, the highest ammonia of phoenix tail chicken content
Base acid is all glutamic acid, is secondly lysine, aspartic acid, leucine, arginine, alanine, threonine, isoleucine, silk
Propylhomoserin, glycine, valine, tyrosine and phenylalanine；The content of proline and histidine is relatively low.Methionine and cystine
Content it is minimum, from testing result as can be seen that the amino acid composition of phoenix tail chicken muscle is formed with the muscle amino acid of white feather chicken
It is almost the same.Illustrate that the amino acid composition of chicken has certain regularity and relative stability, is differentiated with amino acid composition
Or certain species of tracing to the source do not have feasibility.
Embodiment 3
One, micro- type and sample explanation are detected
Detect 15 kinds of elements of Cr, Cu, Fe, Zn, Rb, Mn, Sr, Sn, Mo, Cs, Se, Ba, Co, Ag, Sb in phoenix tail chicken
Content, and the element species and content of itself and commercially available white feather chicken are compared and analyzed.Sample is described as follows：
Phoenix tail chicken chicken leg meat is respectively labeled as：S1、S2、S3；Phoenix tail chicken Fresh Grade Breast is respectively labeled as S4, S5, S6；Totally 6
Sample；White feather chicken chicken leg meat is respectively labeled as：C1、C2、C3；White feather chicken Fresh Grade Breast is respectively labeled as C4, C5, C6；Totally 6 samples
Product, two kinds of kind chickens amount to 12 samples.
Two, detection method and data analysis reference standard
1, method for detecting microelements foundation GB 5009.268-2016 in phoenix tail chicken《National food safety standard food
The measurement of middle multielement》.Using micro-wave digestion inductivity coupled plasma mass spectrometry (ICP-MS) and micro-wave digestion inductive coupling etc.
Ionomer emission spectrum (ICP-OES) two methods measure 15 kinds of elements in Heisui River phoenix tail chicken and commercially available white feather chicken wherein with life
Object constituent analysis-chicken effectively corrects the interference of matrix as internal standard substance.
2, data analysis is carried out with Excel and SPSS statistics softwares.
Three, detecting instrument
German Spike ICP-ARCOS, the silent winged ICAPQ of U.S.'s match
Four, determination of trace elements
1, Preliminary design detection iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), selenium (Se), chromium (Cr), strontium (Sr), cadmium
(Cd), 17 kinds of tin (Sn), caesium (Cs), antimony (Sb), barium (Ba), silver-colored (Ag), cobalt (Co), molybdenum (Mo), rubidium (Rb), tellurium (Te) elements.
The every element of detection is referring especially to GB 5009.268-2016《Multielement in national food safety standard food
It measures》This standard defines inductively coupled plasma mass spectrometry (ICP-MS) and inductive coupling of multi element analysis in food etc.
Ionomer emission spectrum method (ICP-OES).First method suitable for food boron, sodium, magnesium, aluminium, potassium, calcium, titanium, vanadium, chromium, manganese, iron,
The measurement of cobalt, nickel, copper, zinc, arsenic, selenium, strontium, molybdenum, cadmium, tin, antimony, barium, mercury, thallium, lead；Second method be suitable for aluminum in food, boron,
Barium, calcium, copper, iron, potassium, magnesium, manganese, sodium, nickel, phosphorus, strontium, titanium, vanadium, Direct spectrophotometry.
Two kinds of detection modes of ICP-MS and ICP-OES have been used in detection process, avoid the missing inspection of element in phoenix tail chicken.
Last testing result, which is shown within detection limit in phoenix tail chicken, only contains 15 kinds of trace elements.
Five, constituent content difference and composition characteristic in phoenix tail chicken
15 kinds of constituent contents of the white feather chicken to the phoenix tail chicken of Heishui County and purchased from supermarket of Wal-Mart carry out multiple ratio respectively
Compared with analysis, in detection elements content, select biochemical component-chicken standard substance as internal standard, it can with ensure data
By property, testing result shows Mn, Sr, Mo, Cs and Ag in phoenix tail chicken and white feather chicken, and there are significant difference (tables 2), wherein phoenix
Mn, Sr and Ag in tail chicken are significantly higher than white feather chicken, and the Mo and Cs in phoenix tail chicken are substantially less than white feather chicken.Tentatively infer above-mentioned
The element of five kinds of significant difference, potential tracing to the source applied to phoenix tail chicken.
Table 2：Different chicken kind different parts constituent contents (μ g/g)
Six, in phoenix tail chicken micronutrient levels principal component analysis
To there are five kinds of elements of Mn, Sr, Mo, Cs and Ag of significant difference to carry out principal component point in phoenix tail chicken and white feather chicken
Analysis, analysis result are as shown in Figure 1.The chicken leg and pigeon breast sample of phoenix tail chicken are mainly related to the 2nd principal component, and the chicken of white feather chicken
Leg and pigeon breast sample are mainly related to the 1st principal component, the above result shows that can according to above-mentioned five kinds of elements by phoenix tail chicken in vain
Plumage chicken distinguishes.
From table 3 it is observed that the contribution rate of population variance 71.451% carrys out autonomic elements 1,2, the 1st principal component mainly integrates
Cs, Mo element information in chicken, the 2nd principal component mainly integrate Mn element informations.Cs, Mo constituent content are higher in white feather chicken,
So its 1st principal component scores highest, Mn constituent content highests in phoenix tail chicken, so it is the 2nd main at highest scoring.Illustrate sample
Breed difference it is corresponding with constituent content difference, therefore principal component analysis can be by comprehensive mode a variety of members in sample
The information of element is intuitively expressed.
Table 3：The feature value vector of each variable and the contribution rate of accumulative variance in preceding 2 principal components
Seven, in phoenix tail chicken constituent content clustering
Using hierarchical clustering method, clustering, knot are carried out to 5 kinds of elements with significant difference in early period testing result
Fruit such as Fig. 2.When the standard of cluster (distance) is different, cluster result is different.From clustering distance be 600 from cut off dendrogram, sample
It is divided into 2 major class：One kind is phoenix tail chicken, including 5 phoenix tail chicken samples (S-1, S-2, S-4, S-5 and S-6), wherein S-3
Sort out mistake；Second class is white feather chicken, including 6 white feather chicken samples and 1 phoenix tail chicken sample (S-3, C-1, C-2, C-3,
C-4, C-5 and C-6), wherein S-3 sorts out mistake.Although there is 1 sample to occur sorting out mistake, other samples in cluster process
The differentiation in the place of production achieves good effect, and explanation can be classified with above-mentioned 5 kinds of elements.
Eight, in phoenix tail chicken constituent content discriminant analysis
Further to verify differentiation effect of each element content's index to chicken kind, discriminant analysis is carried out, is examined from early period
It surveys 5 kinds of elements with significant difference in result and selects and effective variable is differentiated to kind, establish discrimination model, and verify mould
The validity of type.Discrimination model is established with test set (2/3 sample) in validation test, extrapolation verification collection (1/3 sample), which combines, to be handed over
Pitch the validity of proof method verification model.Wherein model 1-4 indicates phoenix tail chicken chicken leg sample, phoenix tail chicken pigeon breast sample, white respectively
Plumage chicken chicken leg sample and white feather chicken pigeon breast sample.
Model 1 (A)=- 86.261+0.199Mn+0.370Ag-0.552Cs
Model 2 (B)=- 15.283+0.059Mn+0.367Cs+0.088Ag
Model 3 (C)=- 20.251+0.017Mn+0.995Cs-0.005Ag
Model 4 (D)=- 36.722-0.018Mn+1.589Cs-0.062Ag
As can be seen from Table 4, this model is 100% to the whole correct decision rate of test set chicken kind, phoenix tail chicken chicken leg,
The correct decision rate of phoenix tail pigeon breast, white feather chicken leg and white feather chicken chest kind is all 100%.The cross validation results of the model are aobvious
Show, the chicken leg and pigeon breast sample of phoenix tail chicken and white feather chicken sentence category correctly, and kind, which differentiates, has value, discriminant analysis result verification
Mn, Cs and Ag have effective taste to phoenix tail chicken sample.
The result explanation：It is determining the content of sample chicken leg and pigeon breast Mn, Cs and Ag, while determining for comparing
The content of white feather chicken chicken leg and pigeon breast Mn, Cs and Ag carries out discriminant analysis, it will be able to determine sample using model 1-4 and software
Whether it is phoenix tail chicken.
Table 4：The discrimination model of elemental fingerprints information is verified
Note:A. cross validation is carried out to the case in analysis, in cross validation, each case is according to from the case
Function that every other case in addition derives from is classified.
B. correctly classified to 100% in initial packet case.
C. correct classification has been carried out to 83.3% that cross validation is grouped in case.
Embodiment 4
Sample chicken leg and sample pigeon breast and white feather chicken chicken leg and white feather chicken pigeon breast are taken, detects wherein Mn, Cs and Ag respectively
Content：
Table 5：The content (ng/g) of Mn, Cs and Ag in sample chicken leg, sample pigeon breast, white feather chicken chicken leg and white feather chicken pigeon breast
| Mn | Cs | Ag | |
| Sample chicken leg | 481.00 | 21.67 | 232.00 |
| Sample pigeon breast | 248.00 | 22.67 | 54.00 |
| White feather chicken chicken leg | 229.33 | 34.00 | 4.67 |
| White feather chicken pigeon breast | 180.67 | 46.67 | 2.67 |
The content that detection obtains is brought into discriminant analysis model：
Model 1 (A)=- 86.261+0.199Mn+0.370Ag-0.552Cs
Model 2 (B)=- 15.283+0.059Mn+0.367Cs+0.088Ag
Model 3 (C)=- 20.251+0.017Mn+0.995Cs-0.005Ag
Model 4 (D)=- 36.722-0.018Mn+1.589Cs-0.062Ag
Wherein model 1-4 indicates sample chicken leg, sample pigeon breast, white feather chicken chicken leg and white feather chicken pigeon breast respectively.
It is calculated by software analysis and judges that the sample is phoenix tail chicken.
Claims (7)
1. a kind of micro- spectral method traced to the source for phoenix tail chicken, it is characterised in that：Including following methods step：
A, phoenix tail chicken chicken meat sample and white feather chicken chicken meat sample are taken；
B, phoenix tail chicken chicken meat sample and the element species in white feather chicken chicken meat sample and content are measured；
C, the type and content of two kinds of chicken trace elements are obtained by step B, then carries out Multiple range test analysis, obtains two breeders
Content in meat tentatively infers that the trace element of these significant differences can be applied there are the micro- type of significant difference
In tracing to the source for phoenix tail chicken；
D, using Principal Component Analysis and hierarchical clustering method to tentatively inferring the micro member that can be applied to phoenix tail chicken and trace to the source in step C
Element is further analyzed, and verifying these types of trace element can distinguish phoenix tail chicken with white feather chicken；
E, discriminant analysis is carried out, is selected from several trace elements with significant difference and effective variable is differentiated to kind,
Discrimination model is established, and verifies the validity of model, thus obtains that there is effective taste to phoenix tail chicken sample, can be applied to phoenix
The Trace Elements that tail chicken is traced to the source.
2. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1, it is characterised in that：In step
In A, specially：Take 6 phoenix tail chicken samples and 6 white feather chicken samples；Wherein phoenix tail chicken sample by 3 phoenix tail chicken chicken leg samples and
3 phoenix tail chicken pigeon breast sample compositions；Wherein white feather chicken sample is by 3 white feather chicken chicken leg samples and 3 white feather chicken pigeon breast sample sets
At.
3. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1, it is characterised in that：In step
In B, specially：It is sent out using micro-wave digestion inductively coupled plasma atomic mass spectrometry and micro-wave digestion inductively coupled plasma
It penetrates two methods of spectrum ICP-OES and measures phoenix tail chicken and the element species in white feather chicken and content, wherein with biochemical component-
Chicken is as internal standard substance.
4. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1, it is characterised in that：In step
In C, content in described two chicken is Mn, Sr, Mo, Cs and Ag there are the micro- type of significant difference.
5. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1, it is characterised in that：In step
In E, is selected from several trace elements with significant difference and effective variable, which is Mn, Cs and Ag, to be differentiated to kind.
6. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1, it is characterised in that：In step
In E, discrimination model is established in validation test using 2/3 sample as test set, 1/3 sample is combined to intersect and be tested as extrapolation verification collection
Demonstration verifies the validity of model.
7. a kind of micro- spectral method traced to the source for phoenix tail chicken according to claim 1 or 5, it is characterised in that：
In step E, the discrimination model is as follows：
Model 1 (A)=- 86.261+0.199Mn+0.370Ag -0.552Cs
Model 2 (B)=- 15.283+0.059Mn+0.367Cs+0.088Ag
Model 3 (C)=- 20.251+0.017Mn+0.995Cs -0.005Ag
Model 4 (D)=- 36.722-0.018Mn+1.589Cs-0.062Ag
Wherein model 1-4 indicates phoenix tail chicken chicken leg sample, phoenix tail chicken pigeon breast sample, white feather chicken chicken leg sample and white feather chicken chicken respectively
Chest sample.
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