CN108485967A - A kind of cell culture apparatus and the method using its detection cell - Google Patents
A kind of cell culture apparatus and the method using its detection cell Download PDFInfo
- Publication number
- CN108485967A CN108485967A CN201810324126.2A CN201810324126A CN108485967A CN 108485967 A CN108485967 A CN 108485967A CN 201810324126 A CN201810324126 A CN 201810324126A CN 108485967 A CN108485967 A CN 108485967A
- Authority
- CN
- China
- Prior art keywords
- cell culture
- culture apparatus
- thin slice
- window
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
Abstract
The invention belongs to micro-imaging technique fields, more particularly to a kind of cell culture apparatus and application its detect cell method, cell culture apparatus therein is cubic type, including four sides and a bottom surface, first window is offered on bottom surface, be provided in first window with matched the first thin slice for making light transmit of first window shape, the first thin slice is parallel with bottom surface；The second window is offered at least one side, be provided in the second window with matched the second thin slice for making light transmit of the second window shape, the second thin slice is parallel with the side where it.Compared to the prior art, the cell culture apparatus of the present invention uses cubical structure, rather than the mode of the vertical adhesion of two slides, cell culture apparatus is set to be less likely to be damaged, and sample is not easy to reveal, it can ensure that excitation light path and light path are vertical relation simultaneously, to improve the practicability of the cell culture apparatus for mating plate imaging technique.
Description
Technical field
Cell is detected the invention belongs to micro-imaging technique field more particularly to a kind of cell culture apparatus and using it
Method.
Background technology
With the development of science and technology the optical microscopy imaging technology of a new generation has obtained huge development, as mating plate fluorescence is aobvious
Microtechnology.In mating plate fluorescence microscopy, excitation light path is to irradiate sample from side using thin layer light, then from the upper of sample
Fluorescence is detected in portion or lower part, and excitation light path is mutually perpendicular to light path.When traditional inverted microscope detects active somatic cell, make
It is laser co-focusing culture dish, bottom is the slide of 0.17mm, and side is plastic material, and thickness is about 1mm, is had exceeded
The investigation depth of high power objective, in the case where excitation light path and light path are mutually perpendicular to, excitation light source object lens can not be from side
The nearly sample of face paste, therefore it is copolymerized the cell sample culture that burnt Tissue Culture Dish cannot be used for mating plate microtechnic.Prepare light at present
When the cell sample of piece imaging, typically vertically it is sticked together with two sheet glass, the samples such as cell are usually dispersed in solidifying
In glue, it is placed in solid form on horizontal slide, the object lens of wherein excitation light source are attached on the slide of vertical direction, and
The object lens of probe source paste on slide in the horizontal direction.But this method haves the shortcomings that notable：1. being easily damaged；2. sample
Product are easy leakage；3. slide is difficult to keep plumbness, it is difficult control to make the angle of excitation light path and light path.
Invention content
The technical problems to be solved by the invention are to provide a kind of cell culture apparatus and the method using its detection cell,
Aim to solve the problem that the cell culture apparatus for being used for mating plate imaging technique in the prior art is easily damaged, sample is easy leakage, exciting light
Road is difficult to keep vertical problem with light path.
In order to solve the above technical problems, the invention is realized in this way, a kind of cell culture for mating plate imaging technique
Device, the cell culture apparatus are cubic type, including four sides and a bottom surface；
First window is offered on the bottom surface, is provided in the first window matched with the first window shape
The first thin slice that light can be made to transmit, first thin slice are parallel with the bottom surface；
The second window is offered at least one side, is provided in second window and second window-shaped
Matched the second thin slice for making light transmit of shape, second thin slice are parallel with the side where it.
Further, the cell culture apparatus further includes head cover, for buckle closure in the top surface of the cell culture apparatus.
Further, the shape of the first window is round or rectangular.
Further, the shape of second window is round or rectangular.
Further, the material of first thin slice is transparent glass or transparent plastic.
Further, the material of second thin slice is transparent glass or transparent plastic.
Further, the thickness of first thin slice and second thin slice is 0.17mm.
The present invention also provides a kind of method detecting cell using above-mentioned cell culture apparatus, the method includes with
Lower step：
A. cell sample is positioned in the cell culture apparatus；
B. excitation object lens are tightly attached on the lateral surface of the second thin slice of the cell culture apparatus, the excitation object lens are used
In to the cell sample emit exciting light；
C. objective lens are tightly attached on the lateral surface of the first thin slice of the cell culture apparatus, the objective lens are used
In the state for detecting the cell sample.
Compared with prior art, the present invention advantageous effect is：Cell culture apparatus in the present invention is cubic type, packet
Four sides and a bottom surface are included, first window is offered on bottom surface, is provided in first window and is matched with first window shape
Make the first thin slice that light transmits, the first thin slice is parallel with bottom surface；Offer the second window at least one side, second
It is provided in window and matched the second thin slice for making light transmit of the second window shape, side of second thin slice where with it
It is parallel.Compared to the prior art, cell culture apparatus of the invention uses cubical structure, rather than two slides vertically glue
Mode even, makes cell culture apparatus be less likely to be damaged, and sample is not easy to reveal, simultaneously because the first thin slice is parallel with bottom surface,
Second thin slice is parallel with the side where it, and cubical bottom surface is vertical relation with side, if therefore being close to excitation object lens
The outside of second thin slice, objective lens are close to the outside of the first thin slice, then excitation light path and light path are vertical relation, to
Improve the practicability of the cell culture apparatus for mating plate imaging technique.
Description of the drawings
Fig. 1 is the structural schematic diagram of cell culture apparatus provided in an embodiment of the present invention；
Fig. 2 is the structural schematic diagram of another angles of Fig. 1；
Fig. 3 is the structural schematic diagram of the another angles of Fig. 1.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
As shown in Figures 1 to 3, it is a kind of cell culture apparatus provided in an embodiment of the present invention, the cell culture apparatus is main
Applied to mating plate imaging technique, which is cubic type, including a bottom surface 1 and four sides 2.
First window is offered on bottom surface 1, is provided in first window and first window shape is matched keeps light saturating
The first thin slice 101 penetrated, the first thin slice 101 are parallel with bottom surface 1.
The second window is offered at least one side 2, be provided in the second window with the second window shape is matched can
The second thin slice 201 for making light transmit, the second thin slice 201 are parallel with the side 2 where it.It, can according to the needs of different detections
To open up the second window on a side 2, the second window can also be opened up on four sides 2, it can be according to specific needs
It is selected, is not limited herein.
Above-mentioned cell culture apparatus further includes head cover 3, and head cover 3 is used for buckle closure in the top surface of cell culture apparatus, to protect
Hold the cleaning of cell culture apparatus.
The shape of first window and the second window can be designed as round, rectangular, triangle or various other shapes,
It does not limit herein.
The material of first thin slice 101 and the second thin slice 102 can use transparent glass or transparent plastic.
The thickness of first thin slice 101 and the second thin slice 102 may be configured as 0.17mm.
Using the method for cell culture apparatus detection cell in the present embodiment, include the following steps：
A. cell sample is positioned in cell culture apparatus；
B. excitation object lens are tightly attached on the lateral surface of the second thin slice of cell culture apparatus, excitation object lens are used for cell
Electromagnetic radiation exciting light；
C. objective lens are tightly attached on the lateral surface of the first thin slice of cell culture apparatus, objective lens are thin for detecting
The state of born of the same parents' sample.
The method of its detection cell of cell culture apparatus provided in this embodiment and application, cell culture apparatus therein are adopted
With cubical structure, rather than the mode of the vertical adhesion of two slides, so that cell culture apparatus is less likely to be damaged, and sample is not
Easily leakage, simultaneously because the first thin slice is parallel with bottom surface, the second thin slice is parallel with the side where it, cubical bottom surface and side
Face is vertical relation, if therefore object lens will be excited to be close to the outside of the second thin slice, objective lens are close to the outside of the first thin slice, then
Excitation light path is vertical relation with light path, to improve the practicality of the cell culture apparatus for mating plate imaging technique
Property.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (8)
1. a kind of cell culture apparatus, which is characterized in that the cell culture apparatus is cubic type, including four sides and one
A bottom surface；
First window is offered on the bottom surface, is provided in the first window and the first window shape is matched makes
First thin slice of light transmission, first thin slice are parallel with the bottom surface；
The second window is offered at least one side, is provided in second window and second window shape
The second thin slice for making light transmit matched, second thin slice are parallel with the side where it.
2. cell culture apparatus as described in claim 1, which is characterized in that the cell culture apparatus further includes head cover, is used
In buckle closure in the top surface of the cell culture apparatus.
3. cell culture apparatus as described in claim 1, which is characterized in that the shape of the first window is round or side
Shape.
4. cell culture apparatus as described in claim 1, which is characterized in that the shape of second window is round or side
Shape.
5. cell culture apparatus as described in claim 1, which is characterized in that the material of first thin slice be transparent glass or
Person's transparent plastic.
6. cell culture apparatus as described in claim 1, which is characterized in that the material of second thin slice be transparent glass or
Person's transparent plastic.
7. cell culture apparatus as described in claim 1, which is characterized in that the thickness of first thin slice and second thin slice
Degree is 0.17mm.
8. a kind of method of cell culture apparatus detection cell of application as described in claim 1 to 7 any one, feature exist
In the described method comprises the following steps：
A. cell sample is positioned in the cell culture apparatus；
B. will excitation object lens be tightly attached on the lateral surface of the second thin slice of the cell culture apparatus, the excitation object lens for
The cell sample emits exciting light；
C. objective lens are tightly attached on the lateral surface of the first thin slice of the cell culture apparatus, the objective lens are for examining
Survey the state of the cell sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324126.2A CN108485967A (en) | 2018-04-12 | 2018-04-12 | A kind of cell culture apparatus and the method using its detection cell |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810324126.2A CN108485967A (en) | 2018-04-12 | 2018-04-12 | A kind of cell culture apparatus and the method using its detection cell |
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| Publication Number | Publication Date |
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| CN108485967A true CN108485967A (en) | 2018-09-04 |
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| CN201810324126.2A Pending CN108485967A (en) | 2018-04-12 | 2018-04-12 | A kind of cell culture apparatus and the method using its detection cell |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019196043A1 (en) * | 2018-04-12 | 2019-10-17 | 深圳大学 | Cell culture device and method for detecting cell using same |
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| CN105359025A (en) * | 2013-07-10 | 2016-02-24 | 卡尔蔡司显微镜有限责任公司 | Assembly for light sheet microscopy |
| CN105378537A (en) * | 2013-07-10 | 2016-03-02 | 卡尔蔡司显微镜有限责任公司 | Arrangement for light sheet microscopy |
| CN205856488U (en) * | 2016-07-07 | 2017-01-04 | 深圳大学 | A kind of novel cell cultivates sheet |
| WO2017079406A1 (en) * | 2015-11-03 | 2017-05-11 | President And Fellows Of Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
| CN107430264A (en) * | 2015-03-16 | 2017-12-01 | 卡尔蔡司显微镜有限责任公司 | The method and apparatus that mating plate microscope for sample detects |
| CN107430266A (en) * | 2014-12-19 | 2017-12-01 | 卡尔蔡司显微镜有限责任公司 | Method for carrying out the micro- detection of mating plate to sample |
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2018
- 2018-04-12 CN CN201810324126.2A patent/CN108485967A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105359025A (en) * | 2013-07-10 | 2016-02-24 | 卡尔蔡司显微镜有限责任公司 | Assembly for light sheet microscopy |
| CN105378537A (en) * | 2013-07-10 | 2016-03-02 | 卡尔蔡司显微镜有限责任公司 | Arrangement for light sheet microscopy |
| CN107430266A (en) * | 2014-12-19 | 2017-12-01 | 卡尔蔡司显微镜有限责任公司 | Method for carrying out the micro- detection of mating plate to sample |
| CN107430264A (en) * | 2015-03-16 | 2017-12-01 | 卡尔蔡司显微镜有限责任公司 | The method and apparatus that mating plate microscope for sample detects |
| WO2017079406A1 (en) * | 2015-11-03 | 2017-05-11 | President And Fellows Of Harvard College | Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix |
| CN205856488U (en) * | 2016-07-07 | 2017-01-04 | 深圳大学 | A kind of novel cell cultivates sheet |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2019196043A1 (en) * | 2018-04-12 | 2019-10-17 | 深圳大学 | Cell culture device and method for detecting cell using same |
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Application publication date: 20180904 |