CN108485910A - A kind of Double-spiral micro-fluidic chip - Google Patents
A kind of Double-spiral micro-fluidic chip Download PDFInfo
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Abstract
The invention belongs to rare particle enrichments to screen field,It is related to a kind of Double-spiral micro-fluidic chip,Technical barrier for spiral shape microchannel in the sorting enrichment field of Peripheral Blood from Patients with Malignant circulating tumor cell,Dexterously have devised the series connection inertial separation structure of two spiral shape microchannels of reasonable application of the manystage cascade connection,Relative to existing similar technique,The present invention is enriched with Peripheral Circulation tumour cell to sorting,Not only capture rate and processing flux with good circulating tumor cell,The purity of circulating tumor cell can also be maximized,Minimize the loss of circulating tumor cell,And maintain the original phenotype of circulating tumor cell,The cell suspension containing high-purity circulating tumor cell obtained is particularly suitable for subsequent biomedical detection,Genetic analysis,Cell culture,Xenograft tumor preparation etc.,With extensive clinical practice application value.
Description
Technical field
The invention belongs to rare particle enrichments to screen field, be related to a kind of Double-spiral micro-fluidic chip.
Background technology
Circulating tumor cell in the circulatory system not only has morphology and science of heredity heterogeneous but also very rare, even if
It is late tumor patient, the circulating tumor cell in every milliliter of whole blood can only have 1-10, therefore, realize circulating tumor cell
Detection, it is an essential step that sorting enrichment is carried out to it, also, the good and bad of circulating tumor cell sorting enrichment will
Directly affect its subsequent detection result.Relative to linear type and curved arc shape inertia micro flow chip, spiral shape inertia micro flow chip
There is unique advantage in sorting enrichment cycles tumour cell field, and be mainly manifested in and can rapidly process whole blood sample,
Reach the speed of 3mL/min.But existing spiral shape inertia micro flow chip be mainly single channel form single-screw shape it is micro- logical
Road its major drawbacks of inertia micro flow chip are that obtained circulating tumor cell purity is low, after circulating tumor cell
It is continuous to differentiate that with clinical practice application, practical clinical value is relatively low there are bigger difficulty.Although existing spiral shape is micro-
Channel still has larger deficiency and relevant technical bottleneck in terms of sorting enrichment cycles tumour cell, still, existing
Result of study prompts, if it is possible to which dexterously " cascade " applies multiple single spiral shape microchannels, can ensure good prize
Under the precondition of efficiency and processing flux, circulating tumor cell is pure in the cell mixture that the sorting that greatlys improve is enriched to
Degree makes it meet clinical actual demand.
Invention content
In view of this, the purpose of the present invention is to provide a kind of Double-spiral micro-fluidic chips.
In order to achieve the above objectives, the present invention provides the following technical solutions：
A kind of Double-spiral micro-fluidic chip, including two single-screw shape channels, respectively the first single-screw shape channel with
And the second single-screw shape channel；The first single-screw shape channel includes first entrance, first with first entrance sealed connection
Microchannel and the first outlet being tightly connected with the one end of the first microchannel far from first entrance；The first entrance is by
One cell entry and the first sheath fluid entrance converge；The first outlet is by the first waste liquid outlet and the first cell outlet
It converges；The second single-screw shape channel include second entrance, with second entrance be tightly connected the first microchannel and
The second outlet being tightly connected with the one end of the second microchannel far from second entrance；The second entrance by the second cell entry with
And second sheath fluid entrance converge；The second outlet is converged by the first waste liquid outlet and the second cell outlet；Institute
It states and is equipped with pressure balance module at the first waste liquid outlet；It is tightly connected at first cell outlet and the second cell entry；Institute
The flow rate of liquid for stating the second cell entry is v1, and the flow rate of liquid of the first cell entry is v2, the liquid flow of the second cell outlet
Speed is v3, and the flow rate of liquid of first cell outlet is v4；The flow rate of liquid of second waste liquid outlet is v5；Described first
The flow rate of liquid of waste liquid outlet is v6；The pressure balance module is used to make the liquid flow direction and first of the second cell entry
The liquid flow direction of cell entry is consistent, and 0.7*v2≤v1≤1.3*v2；Make the liquid flow direction of the second cell outlet
It is consistent with the liquid flow direction of the first cell outlet, and 0.7*v4≤v3≤1.3*v4, and v1=v4；Make the second waste liquid outlet
Liquid flow direction and the first waste liquid outlet liquid flow direction it is consistent, and 0.7*v6≤v5≤1.3*v6；It is described two
Single-screw shape microchannel forms non-cross spiral shape microchannel, and is fixed at a chip interior；
Wherein, the first cell entry, which is used to enter, contains dilute fine-grained sample liquid；Second cell outlet divides for collecting
Rare particle after choosing enrichment；The first sheath fluid entrance and the second sheath fluid entrance carry osmotic pressure for adding, and ensure dilute
There is the complete dilution of particle.
Optionally, the pressure balance module is to be arranged at the first waste liquid outlet and be tightly connected with the first waste liquid outlet
Pressure balance microchannel.
Optionally, the pressure balance microchannel is arranged in the chip interior.
Optionally, the pressure balance module is the compression pump being arranged in the first waste liquid outlet.
Optionally, the first outlet and second outlet are arranged at outside the geometric shape of the spiral shape microchannel
Portion.
Optionally, the first single-screw shape channel and the second single-screw shape channel are in the cyclic structure being mutually parallel.
Optionally, the horizontal distance between the first single-screw shape channel and the second single-screw shape channel be 0.1~
1000μm。
Optionally, the section in the first single-screw shape channel and the second single-screw shape channel is trapezoidal.
Optionally, the side height of the geometric center of the trapezoidal close spiral shape microchannel is less than the trapezoidal separate spiral shape
The side height of the geometric center of microchannel.
Optionally, the section in the first single-screw shape channel and the second single-screw shape channel is rectangle, the rectangle
Width w and height h ratio w/h=1.2~10.
The beneficial effects of the present invention are：
Sorting of the present invention for spiral shape microchannel in Peripheral Blood from Patients with Malignant circulating tumor cell is enriched with field
Technical barrier, develop more simple and effective technology, realize the cascade superposing type on individual spiral shape inertia micro flow chip
Classification enrichment and/or purification cycle tumour cell.Relative to existing similar technique, the present invention is to sorting enrichment and/or periphery
Blood circulation tumour cell not only has the capture rate and processing flux of good circulating tumor cell, it is also possible to maximum
The purity for changing circulating tumor cell, minimizes the loss of circulating tumor cell, and maintain the original phenotype of circulating tumor cell, pole
The earth reduces testing cost, shortens processing time and flow, and the cell containing high-purity circulating tumor cell obtained is outstanding
Liquid has extensive particularly suitable for subsequent biomedical detection, genetic analysis, cell culture, xenograft tumor preparation etc.
And far-reaching clinical practice application value.
Other advantages, target and the feature of the present invention will be illustrated in the following description to a certain extent, and
And to a certain extent, based on will be apparent to those skilled in the art to investigating hereafter, Huo Zheke
To be instructed from the practice of the present invention.The target and other advantages of the present invention can by following specification realizing and
It obtains.
Description of the drawings
To make the objectives, technical solutions, and advantages of the present invention clearer, the present invention is made below in conjunction with attached drawing excellent
The detailed description of choosing, wherein：
Fig. 1 is a kind of concrete structure schematic diagram of Double-spiral micro-fluidic chip involved in the present invention.
Specific implementation mode
Illustrate that embodiments of the present invention, those skilled in the art can be by this specification below by way of specific specific example
Disclosed content understands other advantages and effect of the present invention easily.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also be based on different viewpoints with application, without departing from
Various modifications or alterations are carried out under the spirit of the present invention.It should be noted that the diagram provided in following embodiment is only to show
Meaning mode illustrates the basic conception of the present invention, and in the absence of conflict, the feature in following embodiment and embodiment can phase
Mutually combination.
Referring to Fig. 1, the element numbers in attached drawing indicate respectively：First cell entry 1, the first sheath fluid entrance 2, first
Entrance A, pressure balance microchannel 3, the first cell outlet 4, the second sheath fluid entrance 5, the first waste liquid outlet 6, first outlet B,
Two waste liquid outlets 7, the second cell outlet 8, second outlet C.
The present invention relates to a kind of Double-spiral micro-fluidic chips, are enriched with rare particle for sorting, wrap in the present embodiment
Two single-screw shape microchannels are included, which is arranged in the same plane and non-crossly winds cyclization
Shape, as shown in Figure 1.
Single single-screw shape microchannel include entrance A, the microchannel being connect with inlet seal and with channel far from enter
The outlet B that one end of mouth is tightly connected, the entrance are converged by cell entry 1 and sheath fluid entrance 2, the outlet B
It is converged by waste liquid outlet 6 and cell outlet 4；
It is equipped with pressure balance microchannel 3 in the first waste liquid outlet 6, the flow rate of liquid of the second cell entry is v1, and first is thin
The flow rate of liquid of born of the same parents' entrance 1 is v2；The flow rate of liquid of second cell outlet 8 is v3, and the flow rate of liquid of the first cell outlet 4 is
v4；The flow rate of liquid of second waste liquid outlet 7 is v5, and the flow rate of liquid of the first waste liquid outlet 6 is v6；It uses the pressure balance microchannel
It is consistent in the liquid flow direction of the liquid flow direction for making the second cell entry and the first cell entry 1, and 0.7*v2≤v1
≤1.3*v2；The liquid flow direction of the liquid flow direction and the first cell outlet 4 that make the second cell outlet 8 is consistent, and
0.7*v4≤v3≤1.3*v4, and v1=v4；Make the liquid of the liquid flow direction and the first waste liquid outlet 6 of the second waste liquid outlet 7
Body flow direction is consistent, and 0.7*v6≤v5≤1.3*v6；
Wherein, the first cell entry 1, which is used to enter, contains dilute fine-grained sample liquid；Second cell outlet 8 is for collecting
Rare particle after sorting enrichment；The first sheath fluid entrance 2 and the sheath fluid entrance 5 in the second single-screw shape channel are for adding
With osmotic pressure, ensure rare particulate integrity and the dilution of biological activity.
Using the form of two single-screw shape microchannels, the second single-screw shape channel can be directly logical to the first single-screw shape
The rare particle that road sorting is enriched to carries out further sorting enrichment, to rarer than single single-screw shape microchannel capture
Particle it is more efficient, be equivalent to and sorting enrichment twice and purifying carried out to rare particle, to the capture rate of target cell,
The quadrinomial parameters such as handling capacity, cell purity, cell activity are superior to single-screw shape microchannel.
Double helix of the present invention is direct through head and the tail by the first single-screw shape channel and the second single-screw shape channel
Connection type constitutes the whole geometry structure that an appearance is Double-spiral microchannel parallel and with identical centre point, special
Sign is that the first entrance A of Double-spiral microchannel is respectively positioned on the outside of microchannel main body geometry with second outlet.
There are cell entry 1, sheath fluid entrance in each single-screw shape channel of Double-spiral micro-fluidic chip of the present invention
2, cell outlet 4 and waste liquid outlet 6, wherein cell entry 4 and sheath fluid entrance 2 pool entering for each single-screw shape microchannel
Mouth A, cell outlet 4 and waste liquid outlet 6 pool the outlet B of each single-screw shape microchannel.Cell entry 1 is usually located at set
The exterior sidewall portions of single-screw shape microchannel entrance, sheath fluid entrance 2 are usually located at the inside of set single-screw shape microchannel entrance
Wall part；Cell outlet 4 is usually located at the inner sidewall part of set single-screw shape microchannel outlet, and waste liquid outlet 6 is usually located at
The exterior sidewall portions of set single-screw shape microchannel outlet.
Two single-screw shape channels of the present invention have the rectangle cross sections of same or similar restriction aspect ratio, or
Madial wall height be less than lateral wall height trapezoid cross section, for because target cell with existing for non-target cell size difference due to only make
Target cell forms focused flow in microchannel madial wall, and non-target cell is then with Dien vortex-like fashion in microchannel madial wall and lateral wall
Between lateral transfer back and forth.Further, the width of microchannel is 0.1-1000 μm, and depth is 0.1-800 μm, or more into one
Step, the width of microchannel is 100-800 μm, and depth is 50-500 μm, or further, the width of microchannel is 600 μm, depth
It is 140-180 μm.
Two single-screw shape microchannels of Double-spiral micro-fluidic chip of the present invention have same or similar length,
It is characterized in that the length of the spiral shape microchannel starting point of the 1st and the 2nd single-screw shape microchannel to terminal is non-target cell
The 1.7-2.0 Dien period of hydrodynamics characteristic, alternatively, 2.7-3.0 Dien period, alternatively, 3.7-4.0 Dien week
Phase, alternatively, 4.7-5.0 Dien period, alternatively, 5.7-6.0 Dien period, alternatively, 6.7-7.0 Dien period, alternatively,
7.7-8.0 Dien period, alternatively, 8.7-9.0 Dien period, alternatively, 9.7-10.0 Dien period.With this condition,
In first and the terminal point in the second single-screw shape channel, non-target cell is along dien vertex stream migration to the outside of spiral shape microchannel
Wall part.
The waste liquid outlet in the first single-screw shape channel of Double-spiral micro-fluidic chip of the present invention has pressure balance micro-
Channel 3, the size and shape of above-mentioned pressure balance microchannel 3 of the invention are without particular/special requirement, to its aspect ratio, curvature half
Without particular/special requirement, effect is to balance the pressure of the first cell outlet 1, makes the second single-screw shape for diameter, width, height and length
The flow velocity of the cell entry in channel or the cell outlet 4 in the first single-screw shape channel and direction and the first single-screw shape channel
The flow velocity of cell entry 1 and the cell outlet 4 in the second single-screw shape channel is identical as direction holding or extremely close, meanwhile, make
Flow velocity and side of the flow velocity of the waste liquid outlet 6 in one single-screw shape channel with direction with the waste liquid outlet 7 in the second single-screw shape channel
It is identical or extremely close to holding.
The horizontal parallel spacing of first and second spirality channels of Double-spiral micro-fluidic chip of the present invention is
0.1-1000μm。
The width of 1 microchannel of the first and/or second cell entry of Double-spiral micro-fluidic chip of the present invention is micro-
The width of the microchannel of the waste liquid outlets of the 10-40% of channel overall width, first and/or second 6 is microchannel overall width
40-90%.
Blood sample of the present invention refers to the blood sample for having certain hematid specific volume, alternatively, passing through haemolysis side
Formula removes the blood sample of a large amount of red blood cells, alternatively, by other approach well knowns or device obtain without red blood cell or
Only a small amount of or minute quantity red blood cell blood sample.
Sheath fluid of the present invention refers to any type this field for having certain osmotic pressure, and not destroying haemocyte integrality
Well known various solution, such as phosphate buffer, culture serum, physiological saline.
Can particle form the size and radius of curvature, particle that inertia focuses flowing and microchannel in curved arc shape microchannel
Size and the factors such as flow velocity have relationship.Existing research shows that the width in channel is more than the rectangular section channel of height
In, the particle of certain size forms inertia and focuses flowing it is generally necessary to meet the diameter that condition a/h >=0.07, a are particles, and h is
The minimum dimension of microchannel, the height of microchannel is typically minimum dimension in inertia micro flow chip.It, can if particle size is too small
Particle can be caused inertia can not to be focused in the microchannel of finite length.Also some researches show that a/h<0.5 is also restrictive condition,
The excessive channel that may result in of particle size is easy to block.It therefore, be according to the size of separating particles come really when designing preparation
Determine the size of microchannel.
The operation principle of Double-spiral micro-fluidic chip of the present invention is as follows：At first entrance A, blood sample is certainly
First cell entry 1 enters microchannel, and sheath fluid enters microchannel from the first sheath fluid entrance 2, due to the fluid of the first sheath fluid entrance 2
Flow velocity is higher than the fluid flow rate of the first cell entry 1, and all cells of the first cell entry 1 are extruded into the first single-screw shape
The lateral wall in channel, and thus enter subsequent microchannel, therefore, it can be stated that, at 0 Dien period, all cells rise
Beginning position is the lateral wall in the first single-screw shape channel.Under the collective effect of inertia lift and Dien drag force, target cell due to
Its size is larger, and at 0.5 Dien period, the madial wall in the first single-screw shape channel, which is formed, focuses a fluid stream, and due to it
The effect of inertia lift and Dien drag force, target cell remain in the first subsequent microchannel in single-screw shape channel in it
The focused flow of side wall；Non-target cell is mainly acted on by Dien drag force because its size is smaller, the in the form of Dien is vortexed
The lateral wall in one single-screw shape channel and madial wall lateral transfer back and forth, also, it is located at first when typically 0.5 Dien period
The madial wall in single-screw shape channel, and its initial position is back at 1 complete Dien period, i.e. the first single-screw shape is logical
The lateral wall in road.Since there are certain proportions with the above-mentioned Dien period for the length in the first single-screw shape channel, in its exit, target
Cell positioned at the first cell outlet 4 from flowing out and being directly entered the second cell entry, and non-target cell fluid is then from positioned at first
Waste liquid outlet 5 is flowed out and is collected through pressure balance microchannel 3；Since the first cell outlet 4 is the second cell entry,
In second single-screw shape channel, according to the identical operation principle in the first single-screw shape channel, the first single-screw shape channel sorting
The target cell being enriched to is sorted in the second single-screw shape channel by the 2nd sorting enrichment or tandem type sorting enrichment or tandem
Enrichment or superposing type sorting enrichment.It can thus be stated that the Double-spiral micro-fluidic chip described in through the invention, blood sample
In target cell be cascaded or tandem carried out the sorting enrichment of 2 superposing types, simultaneously as most of non-target cell is
By the first single-screw shape channel remove, into the second single-screw shape channel cell suspension total number of cells on the whole well below
The total number of cells of the cell suspension in the second single-screw shape channel, to which, because the interference of its density is few, sorting is enriched with due to cell
Effect can be more preferable than single spiral shape microchannel is used alone, and can finally obtain the target cell of high-purity.
In specific experiment, the width l of spiral shape microchannel is 600 μm, radius of curvature 1.15cm, microchannel height h
It is 130 μm.Traditional single-screw shape microchannel is in spiral planar inside configuration design entry, and planar structure build up outside goes out
Mouthful, the width of cell entry and cell outlet is 0.15mm, is located at the lateral wall and madial wall of spiral shape microchannel, sheath
The width of liquid entrance and waste liquid outlet is 0.45mm, is located at the madial wall and lateral wall of spiral shape microchannel, and cell enters
Mouth and waste liquid entrance are respectively positioned on the inside of spiral shape microchannel planar geometry, and cell outlet and sheath fluid outlet are respectively positioned on spiral
The outside of shape microchannel planar geometry.
The cell entry 1 of Double-spiral micro-fluidic chip of the present invention and the width of cell outlet 4 are 0.15mm,
The width of sheath fluid entrance 2 and waste liquid outlet 6 is 0.45mm；There is cell entry 1, sheath fluid entrance 5, thin in each single-screw shape channel
Born of the same parents outlet 4 and waste liquid outlet 6, wherein cell entry 1 and sheath fluid entrance 2 pool the entrance of each single-screw shape microchannel, carefully
Born of the same parents outlet 4 and waste liquid outlet 6 pool the outlet B of each single-screw shape microchannel；It is micro- that cell entry 1 is located at set single-screw shape
The exterior sidewall portions of feeder connection, sheath fluid entrance 2 are located at the inner sidewall part of set single-screw shape microchannel entrance；Cell outlet
4 are located at the inner sidewall part of set single-screw shape microchannel outlet, and waste liquid outlet 6 is located at the outlet of set single-screw shape microchannel
Exterior sidewall portions.
The particle purity that the lower record different in flow rate of table one is collected into
| Flow velocity | Double helix | Single-screw |
| 150μl/min | 54.56±1.12 | 34.58±5.28 |
| 300μl/min | 76.24±2.35 | 42.56±5.81 |
| 450μl/min | 86.10±2.84 | 62.57±6.12 |
| 600μl/min | 94.12±2.08 | 67.60±7.27 |
| 750μl/min | 99.08±0.43 | 70.36±8.76 |
| 900μl/min | 97.02±2.47 | 54.56±5.21 |
Doubling dilution is distinguished to 10 using HCC827, MCF-7, SW480 tumor cell line manually cultivated2A cell/ml,
According to 1:5 volume ratio is mixed into using erythrocyte cracked liquid treated normal plasma cell, using Double-spiral of the present invention
Micro-fluidic chip carries out sorting enrichment, and testing result is shown in Table two using the count results of immunofluorescence technique stain smear.As a result
Show that Double-spiral micro-fluidic chip of the present invention is at least up to 98% or more, and purity to the rate of recovery of kinds of tumor cells
It is similar to above-mentioned Double-spiral microchannel, it is each about 99%.
Two variety classes tumour cell of table using Double-spiral microchannel be enriched with after the rate of recovery
Compared with traditional single-screw shape microchannel, the time of Double-spiral micro-fluidic chip detection of the present invention shortens
To the 1/4 of traditional single-screw shape microchannel, and sorts the dilute fine-grained purity being enriched to, the repeatability of experimental result and return
The methods of yield learns parameter and is far superior to traditional single-screw shape microchannel, illustrates relative to traditional single-screw shape microchannel,
Double-spiral micro-fluidic chip of the present invention can preferably meet the actual demand of clinical application.
Finally illustrate, the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although with reference to compared with
Good embodiment describes the invention in detail, it will be understood by those of ordinary skill in the art that, it can be to the skill of the present invention
Art scheme is modified or replaced equivalently, and without departing from the objective and range of the technical program, should all be covered in the present invention
Right in.
Claims (10)
1. a kind of Double-spiral micro-fluidic chip, it is characterised in that：
Including two single-screw shape channels, respectively the first single-screw shape channel and the second single-screw shape channel；
The first single-screw shape channel includes first entrance, the first microchannel being tightly connected with first entrance and with the
The first outlet that the one end of one microchannel far from first entrance is tightly connected；
The first entrance is converged by the first cell entry and the first sheath fluid entrance；
The first outlet is converged by the first waste liquid outlet and the first cell outlet；
The second single-screw shape channel includes second entrance, the first microchannel being tightly connected with second entrance and with the
The second outlet that the one end of two microchannels far from second entrance is tightly connected；
The second entrance is converged by the second cell entry and the second sheath fluid entrance；
The second outlet is converged by the first waste liquid outlet and the second cell outlet；
Pressure balance module is equipped at first waste liquid outlet；
It is tightly connected at first cell outlet and the second cell entry；
The flow rate of liquid of second cell entry is v1, and the flow rate of liquid of the first cell entry is v2, the second cell outlet
Flow rate of liquid is v3, and the flow rate of liquid of first cell outlet is v4；The flow rate of liquid of second waste liquid outlet is v5；Institute
The flow rate of liquid for stating the first waste liquid outlet is v6；
The pressure balance module is used to make the liquid flow direction of the second cell entry and the liquid of the first cell entry to flow
Direction is consistent, and 0.7*v2≤v1≤1.3*v2；Make the liquid of the liquid flow direction and the first cell outlet of the second cell outlet
Body flow direction is consistent, and 0.7*v4≤v3≤1.3*v4, and v1=v4；Make the liquid flow direction and of the second waste liquid outlet
The liquid flow direction of one waste liquid outlet is consistent, and 0.7*v6≤v5≤1.3*v6；
Described two single-screw shapes microchannel forms non-cross spiral shape microchannel, and is fixed at a chip interior；
Wherein, the first cell entry, which is used to enter, contains dilute fine-grained sample liquid；Second cell outlet is rich for collecting sorting
Rare particle after collection；The first sheath fluid entrance and the second sheath fluid entrance carry osmotic pressure for adding, and ensure rare micro-
The complete dilution of grain.
2. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The pressure balance module is setting
The pressure balance microchannel being tightly connected at the first waste liquid outlet and with the first waste liquid outlet.
3. Double-spiral micro-fluidic chip as described in claim 2, it is characterised in that：The pressure balance microchannel setting
In the chip interior.
4. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The pressure balance module is setting
In the compression pump of the first waste liquid outlet.
5. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The first outlet and second go out
Mouth is arranged at outside the geometric shape of the spiral shape microchannel.
6. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The first single-screw shape channel with
Second single-screw shape channel is in the cyclic structure being mutually parallel.
7. Double-spiral micro-fluidic chip as described in claim 6, it is characterised in that：The first single-screw shape channel with
Horizontal distance between second single-screw shape channel is 0.1~1000 μm.
8. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The first single-screw shape channel with
The section in the second single-screw shape channel is trapezoidal.
9. Double-spiral micro-fluidic chip as described in claim 8, it is characterised in that：The trapezoidal close spiral shape microchannel
Geometric center side height less than the trapezoidal separate spiral shape microchannel geometric center side height.
10. Double-spiral micro-fluidic chip as described in claim 1, it is characterised in that：The first single-screw shape channel
Section with the second single-screw shape channel is rectangle, ratio w/h=1.2~10 of the width w and height h of the rectangle.
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