CN108484788A - A kind of extraction and separation process of algal polysaccharide extract - Google Patents
A kind of extraction and separation process of algal polysaccharide extract Download PDFInfo
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- CN108484788A CN108484788A CN201810399306.7A CN201810399306A CN108484788A CN 108484788 A CN108484788 A CN 108484788A CN 201810399306 A CN201810399306 A CN 201810399306A CN 108484788 A CN108484788 A CN 108484788A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The present invention relates to a kind of extraction and separation process of algal polysaccharide extract, belong to purification technique field.Technical solution is：It comprises the steps of：Chopping, immersion：The brown algas such as dry undaria pinnitafida, dry sargassum fusifome, dry kelp are taken, are infiltrated, brown alga silk is cut into, drinking water, 25 60 DEG C of soaking temperature, stirring extraction 5h 10h is added；Centrifugation, separation：By mixing liquid centrifugation, separation, filtering and impurity removing, filtrate is obtained；Concentration：Filtrate is concentrated with ultrafiltration membrane；Acidification：Concentrate is stirred down, hydrochloric acid is added, material liquid pH is adjusted and stablizes to 24,6h 10h are stood after stirring 30 min；Press filtration, separation：Liquid carries out press filtration, separation, removes impurity, obtains filtrate；Concentration, washing：Filtrate is concentrated, it is 68 to adjust pH with lye, and purified water is added to be washed；It is dry：Concentrate is subjected to microwave drying, obtains brown alga extract solid.The present invention greatly reduces cost relative to alcohol precipitating method etc., is dried with microwave method, and operating procedure is greatly saved, and improves efficiency.
Description
Technical field
The present invention relates to a kind of extraction and separation process of algal polysaccharide extract, belong to purification technique field.
Background technology
Algal polysaccharide extract has the function of anticoagulation, reducing blood lipid, antitumor and AntiHIV1 RT activity.Prevent region thyroid gland
It is swollen, cholesterol is significantly reduced, the absorption of calcium is increased, to hypertension, artery sclerosis and liposis have certain prevention and auxiliary
Help therapeutic effect.It is good to treatment nephrotic syndrome and morning, mid-term chronic kidney hypofunction effect, it has no toxic side effect, especially to improving kidney function
It is particularly evident, to reduce serum creatinine effect.Algal polysaccharide extract or a kind of good cosmetic material, pass through bioactivation
The potent skin that compacts of moisturizing compound prescription.It can be described as a calm and all apparent moisture-keeping function skin care raw material.But often
Alcohol precipitating method alcohol consumption quantity is big, and production cost is high, and the operation cycle is long, and finished product stability is poor.
Invention content
It is an object of the present invention to provide a kind of extraction and separation process of algal polysaccharide extract, greatly reduce cost, save
Operating procedure, solves the above problem present in background technology.
The technical scheme is that：
A kind of extraction and separation process of algal polysaccharide extract, comprises the steps of：
（1）Chopping, immersion：The brown algas such as dry undaria pinnitafida, dry sargassum fusifome, dry kelp are taken, are infiltrated, brown alga silk is cut into, dry brown alga is added
The drinking water of 10-15 times of weight, 25-60 DEG C of soaking temperature, stirring extraction 5h-10h；
（2）Centrifugation, separation：By step（1）Mixing liquid centrifugation, separation, filtering and impurity removing, obtain filtrate；
（3）Concentration：By step（2）Obtained filtrate is concentrated with ultrafiltration membrane；
（4）Acidification：By step（3）Under obtained concentrate stirring, hydrochloric acid is added, adjusts material liquid pH and stablizes to 2-4, stirring 30
6h-10h is stood after min；
（5）Press filtration, separation：Step（4）Liquid carry out press filtration, separation, remove impurity, obtain filtrate；
（6）Concentration, washing：By step（5）Obtained filtrate is concentrated, and it is 6-8 to adjust pH with lye, and purified water is added to be washed
It washs；
（7）It is dry：By step（6）Concentrate carries out microwave drying, obtains brown alga extract solid.
The positive effect of the present invention is：Cost is greatly reduced relative to alcohol precipitating method etc., is dried with microwave method, significantly
Operating procedure is saved, efficiency is improved.
Specific implementation mode
The invention will be further described with reference to embodiments.
A kind of extraction and separation process of algal polysaccharide extract, comprises the steps of：
（1）Chopping, immersion：The brown algas such as dry undaria pinnitafida, dry sargassum fusifome, dry kelp are taken, are infiltrated, brown alga silk is cut into, dry brown alga is added
The drinking water of 10-15 times of weight, 25-60 DEG C of soaking temperature, stirring extraction 5h-10h；
（2）Centrifugation, separation：By step（1）Mixing liquid centrifugation, separation, filtering and impurity removing, obtain filtrate；
（3）Concentration：By step（2）Obtained filtrate is concentrated with ultrafiltration membrane；
（4）Acidification：By step（3）Under obtained concentrate stirring, hydrochloric acid is added, adjusts material liquid pH and stablizes to 2-4, stirring 30
6h-10h is stood after min；
（5）Press filtration, separation：Step（4）Liquid carry out press filtration, separation, remove impurity, obtain filtrate；
（6）Concentration, washing：By step（5）Obtained filtrate is concentrated, and it is 6-8 to adjust pH with lye, and purified water is added to be washed
It washs；
（7）It is dry：By step（6）Concentrate carries out microwave drying, obtains brown alga extract solid.
Embodiment one：
The algal polysaccharide extract of the present embodiment, input raw material weight are：Dry kelp 1000 kg, drinking water 10m3, purified water
5.0m3, hydrochloric acid 60kg, sodium hydroxide 0.8kg.
（1）Chopping, immersion：Dry kelp 1000kg is taken, is infiltrated, the Shredded kelp of 2-5mm width is cut into, 10m is added3Drink
Water, 35 ± 5 DEG C of soaking temperature, stirring extraction 8h；
（2）Centrifugation, separation：By step（1）Mixing liquid centrifugation, separation, filtering and impurity removing, obtain filtrate；
（3）Concentration：By step（2）Obtained filtrate is concentrated with ultrafiltration membrane, concentrate solution to the 1/3 to 1/2 of original volume；
（4）Acidification：By step（3）Under obtained concentrate stirring, hydrochloric acid is added, adjusts material liquid pH and stablizes to 2-4, stirring 30
7h-10h is stood after min；
（5）Press filtration, separation：Step（4）Liquid carry out press filtration, separation, remove impurity, obtain filtrate；
（6）Concentration, washing：By step（5）Obtained filtrate is concentrated, and it is 6-8 to adjust pH with lye, and purified water is added to carry out
Washing；
（7）It is dry：By step（6）Concentrate carries out microwave drying, obtains brown alga extract solid.
Through assay, algal polysaccharide sulfate content reaches 85%-90% in solid product, and hydrolysis sulfate content reaches
To 22%-25%.
Embodiment two：
The algal polysaccharide extract of the present embodiment, input raw material weight are：Dry undaria pinnitafida 1000kg, drinking water 15m3, purified water
5.0m3, hydrochloric acid 60kg, sodium hydroxide 0.8kg.
Extracting method is the same as embodiment one
Through assay, algal polysaccharide sulfate content reaches 70%-85% in solid product, and hydrolysis sulfate content reaches
20%-25%.
Claims (1)
1. a kind of extraction and separation process of algal polysaccharide extract, it is characterised in that comprise the steps of：
（1）Chopping, immersion：The brown algas such as dry undaria pinnitafida, dry sargassum fusifome, dry kelp are taken, are infiltrated, brown alga silk is cut into, dry brown alga is added
The drinking water of 10-15 times of weight, 25-60 DEG C of soaking temperature, stirring extraction 5h-10h；
（2）Centrifugation, separation：By step（1）Mixing liquid centrifugation, separation, filtering and impurity removing, obtain filtrate；
（3）Concentration：By step（2）Obtained filtrate is concentrated with ultrafiltration membrane；
（4）Acidification：By step（3）Under obtained concentrate stirring, hydrochloric acid is added, adjusts material liquid pH and stablizes to 2-4, stirring 30
6h-10h is stood after min；
（5）Press filtration, separation：Step（4）Liquid carry out press filtration, separation, remove impurity, obtain filtrate；
（6）Concentration, washing：By step（5）Obtained filtrate is concentrated, and it is 6-8 to adjust pH with lye, and purified water is added to be washed
It washs；
（7）It is dry：By step（6）Concentrate carries out microwave drying, obtains brown alga extract solid.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810399306.7A CN108484788A (en) | 2018-04-28 | 2018-04-28 | A kind of extraction and separation process of algal polysaccharide extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810399306.7A CN108484788A (en) | 2018-04-28 | 2018-04-28 | A kind of extraction and separation process of algal polysaccharide extract |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109705232A (en) * | 2018-12-28 | 2019-05-03 | 金耀仑 | A kind of extraction process of low-molecular-weight algal carbohydrate gum and its application in cosmetics |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197674A (en) * | 1998-05-29 | 1998-11-04 | 青岛海洋大学 | Preparation of polyfucose sulfate with varible molecular weight |
| CN101659709A (en) * | 2009-09-18 | 2010-03-03 | 集美大学 | Preparation method of fucosan |
| CN106046193A (en) * | 2016-07-26 | 2016-10-26 | 东北师范大学 | Seaweed polysaccharide P155 and preparation process thereof |
-
2018
- 2018-04-28 CN CN201810399306.7A patent/CN108484788A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197674A (en) * | 1998-05-29 | 1998-11-04 | 青岛海洋大学 | Preparation of polyfucose sulfate with varible molecular weight |
| CN101659709A (en) * | 2009-09-18 | 2010-03-03 | 集美大学 | Preparation method of fucosan |
| CN106046193A (en) * | 2016-07-26 | 2016-10-26 | 东北师范大学 | Seaweed polysaccharide P155 and preparation process thereof |
Non-Patent Citations (3)
| Title |
|---|
| 丁晓平等: ""海带中提取岩藻多糖"", 《广州化工》 * |
| 张向宇: "《实用化学手册》", 31 October 2011, 国防工业出版社 * |
| 马爱霞等: "《药品GMP车间实训教程 上册》", 30 June 2016, 中国医药科技出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109705232A (en) * | 2018-12-28 | 2019-05-03 | 金耀仑 | A kind of extraction process of low-molecular-weight algal carbohydrate gum and its application in cosmetics |
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Application publication date: 20180904 |