CN108479874A - A kind of electric pipettor - Google Patents
A kind of electric pipettor Download PDFInfo
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- CN108479874A CN108479874A CN201810516532.9A CN201810516532A CN108479874A CN 108479874 A CN108479874 A CN 108479874A CN 201810516532 A CN201810516532 A CN 201810516532A CN 108479874 A CN108479874 A CN 108479874A
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- 239000007788 liquid Substances 0.000 claims abstract description 97
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- 238000009826 distribution Methods 0.000 description 10
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- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
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- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/061—Counting droplets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
Abstract
A kind of electric pipettor, including：Micro air pump, for generating negative pressure；Solenoid valve, when being connected to, powering off with micro air pump for controlling the pipeline that is connected with micro-fluidic chip when powering on and atmosphere；Displacement driver so that liquid is sprayed with drop form in micro-fluidic chip for passing through rigid extension device to one impact force of micro-fluidic chip；Micro-fluidic chip, for drawing, storing and spraying liquid；Control system, for controlling micro air pump, solenoid valve and the working condition of displacement driver；The cavity for storing liquid and can be tapped is provided in micro-fluidic chip；The micro-spraying hole of microchannel and cavity perforation is provided on micro-fluidic chip, the setting of micro-spraying hole meets condition：So that liquid has latching characteristics in micro-fluidic chip.The droplet size for impacting generation is small, and consistency is good.Distributed liquid volume is codetermined by single droplet size and number of shocks, therefore, with higher resolution ratio and precision.
Description
Technical field
The present invention relates to metrical instrument technical fields, and in particular to a kind of high-precision electric pipettor for drawing, divides
With micro liquid.
Background technology
In medical treatment, drug, gene and protein research, biological study, drug development laboratories and other biotechnologys
In application field, it is often necessary to mainly sharp using pipettor come operation laboratory sample in various laboratory procedures
With pipettor by the liquid sucking pipette of certain volume, liquid is then distributed into several required volume of liquid.
Currently, common manual pipettor generally realizes suction and the release (distribution) of liquid by way of piston movement.
Electric pipettor usually changes the air pressure in suction nozzle come pumping fluid by motor or negative pressure pump.For example, Chinese patent
CN201310485164.3《Electric pipettor and its automatic gauge method, automatic liquid separation method》It describes a kind of by controlling suction
Amount intraductal atmospheric pressure is come the method for quantification extraction and distribution to realize liquid, although this method, which can be realized, automatically controls suction
Liquid and drain, but its collective effect based on pipeline liquid dead weight and negative pressure, are only applicable to the distribution of larger volume liquid.China
Patent CN 201120338698.X《The control system of high-precision electrical pipettor》A kind of push by stepper motor is described to live
Plug moves to realize that high-precision imbibition and drain, precision mainly have with the step angle of stepper motor and piston cross-section
It closes.Rainin house journals US6254832B1 and CN1300239A, principle are also to push piston to complete liquid by stepper motor
The absorption and distribution of body.The present invention has significant difference, and precision higher of the present invention, droplet size minimum with its principle and structure
It can reach 0.1 nanoliter to 1 nanoliter.High-precision pipettor is largely all based on air slide principle at present, due to the pressure of air
Contracting is acted on suction nozzle surface by capillary force, electrostatic force with micro liquid so that reliable operation condition be limited to 1 microlitre with
On.It is nominal accurate when distributing 1 microlitre of volume of liquid such as the LTS E4-10XLS+ high-precision electrical pipettors of Rainin companies
Spend error ± 2.5%, random error ± 1.2%.For another example the Eppendorf Xplorer Series of High Accuracies of Eppendorf companies
Electric pipettor, minimum allocation liquid volume is 1 microlitre, when distributing 1 microlitre of volume of liquid, accuracy rating error ±
2.5%, random error ± 1.8%.But in drug development, biochemical test, required sample is very precious sometimes, and to liquid
The required precision of sample and reagent is very harsh, and current existing pipettor is difficult to meet the requirements, and therefore, is badly in need of a kind of high-precision
Pipettor.
Invention content
In view of this, an embodiment of the present invention provides a kind of high-precision electric pipettors.
To achieve the above object, the embodiment of the present invention provides the following technical solutions：
A kind of electric pipettor, including：
Micro air pump, solenoid valve, displacement driver, micro-fluidic chip and control system；
The micro air pump provides power for generating negative pressure to draw liquid；
The solenoid valve, the pipeline that control is connected with micro-fluidic chip when powering on are connected to micro air pump, when power is off
Control the pipeline and atmosphere being connected with micro-fluidic chip；
Institute's displacement drive, at work, by along the non-telescoping rigid extension device of the direction of motion to miniflow
It controls the cavity in chip and provides an impact force so that the micro-fluidic chip drips drop；
The micro-fluidic chip can abandon suction nozzle to draw, store and spray liquid for serving as；
The control system, for controlling micro air pump, solenoid valve and the working condition of displacement driver；
The cavity for storing liquid and can be tapped is provided in the micro-fluidic chip；
Deformable films are covered on cavity；
The micro-spraying hole of microchannel and cavity perforation is provided on the micro-fluidic chip, the micro-spraying hole is set
Set the condition of satisfaction：So that liquid has latching characteristics in micro-fluidic chip.
Preferably, in above-mentioned electric pipettor, the control system includes：
Controller and input keyboard；
The first interface of the controller is connected with solenoid valve, to be exported to solenoid valve for controlling electromagnetic valve work state
Control signal；
The second interface of the controller is connected with institute displacement drive, for being used for institute's displacement drive output
Control the control signal that the rigid extension device impacts the deformable films；
The input keyboard and the controller, for be arranged the control signal duration of the controller output, frequency and
Number.
Preferably, in above-mentioned electric pipettor, a circular cylindrical cavity structure, the cylinder are provided on the microchannel
Cavity body structure is used to bear the impact of the rigid extension device.
Preferably, in above-mentioned electric pipettor, institute's displacement drive is electromagnetic driver or piezoelectric actuator.
Preferably, in above-mentioned electric pipettor, the power supply of the electric pipettor is built-in power or external power supply.
Preferably, in above-mentioned electric pipettor, including n groups micro air pump, solenoid valve, displacement driver and micro-fluidic core
Piece, the n are the natural number not less than 2；
The n groups micro air pump, solenoid valve, displacement driver and micro-fluidic chip share a set control system.
Preferably, in above-mentioned electric pipettor, including n groups displacement driver, micro-fluidic chip, the n are not less than 2
Natural number；
The n groups displacement driver and micro-fluidic chip share one group of micro air pump, solenoid valve and control system.
Preferably, in above-mentioned electric pipettor, the electric pipettor include n groups displacement driver, micro-fluidic chip and
Solenoid valve, the n are the natural number not less than 2；Shared one group of the n groups displacement driver, micro-fluidic chip and solenoid valve is micro-
Type air pump and control system.
Preferably, in above-mentioned electric pipettor, the micro air pump, solenoid valve, displacement driver and controller are mounted on
In electric pipettor shell, the input keyboard is arranged on the surface of the electric pipettor shell, the micro-fluidic chip with
It is removably mounted the end point of the shell.
Preferably, in above-mentioned electric pipettor, the shell is tapering type strip structure, and the micro-fluidic chip setting exists
At the necking of the shell.
Preferably, in above-mentioned electric pipettor, the input keyboard is touch keyboard.
Based on the above-mentioned technical proposal, technical solution provided in an embodiment of the present invention is as it can be seen that the electric pipettor is using micro-
Type air pump forms negative pressure as the power for drawing liquid, draws liquid into the cavity of micro-fluidic chip, can be by controlling electricity
The switch time of magnet valve and the uptake that control liquid may be implemented using the motor speed for controlling micro air pump are controlled,
It utilizes displacement driver to impact the micro-fluidic chip after drawing liquid, the liquid in chip is arranged in the form of uniform drop
Go out micro-fluidic chip, also, since the liquid volume of each drop is substantially coincident, it can be driven by controlling electromagnetism
Dynamic device impacts the number of micro-fluidic chip to control the amount of droplets of drippage and the amount of the drop always dripped.Single droplet size
Small (0.1nL-10nL), and it is good to print generation droplet size consistency, it can be seen that, electric pipettor provided by the present application has
Higher resolution ratio and precision.
Description of the drawings
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
The embodiment of invention for those of ordinary skill in the art without creative efforts, can also basis
The attached drawing of offer obtains other attached drawings.
Fig. 1 is a kind of high-level schematic functional block diagram of electric pipettor disclosed in the embodiment of the present application；
Fig. 2 is a kind of structural schematic diagram of electric pipettor disclosed in the embodiment of the present application；
Fig. 3 shows for a kind of single channel electric pipettor imbibition disclosed in the embodiment of the present application and the operating process of distribution liquid
It is intended to；
Fig. 4 is that respectively there are one the electric pipettor knots of air pump for a kind of multichannel and each channel disclosed in the embodiment of the present application
Structure schematic diagram；
Fig. 5 is a kind of electric pipettor structural schematic diagram of the shared air pump of multichannel disclosed in the embodiment of the present application；
Fig. 6 is a kind of electric pipettor structural representation of multichannel shared air pump and solenoid valve disclosed in the embodiment of the present application
Figure.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
It, can be micro- with automatic sucking in order to meet requirements for high precision this application provides a kind of high-precision electric pipettor
Quantity of fluid volume, then can also continuously generate micro-scale volume drop, volume of liquid needed for the absorption of quantification and needed for generating
Dispensed volume liquid, referring to Fig. 1 and Fig. 2, which may include：
Micro air pump 1, solenoid valve 2, displacement driver 3, micro-fluidic chip 4 and control system 5；
The micro air pump 1 is used to generate negative pressure at work, dynamic to be provided for electric pipettor absorption liquid
Power；
The solenoid valve 2, there are three ports for the solenoid valve 2, are denoted as first port, second port and third port,
The pipeline connection that middle first port is connected with the micro-fluidic chip 4, second port are connected to the micro air pump 1, third end
Mouth is connected to external environment, and when the solenoid valve 2 powers on, the first port is connected with second port so that micro-fluidic
The pipeline of chip connection is connected to micro air pump 1, is surged so that the micro-fluidic chip 4 obtains taking for the offer of micro air pump 1
Power, when the solenoid valve 2 powers off, the first port is connected with third port, so that the pipe of micro-fluidic chip connection
Road and atmosphere, prepare for dropping liquid；
Institute's displacement drive 3, at work, by along the non-telescoping rigid extension device of the direction of motion to miniflow
It controls the cavity in chip 4 and provides an impact force so that the micro-fluidic chip drips drop, wherein the unique drive
It can be set according to user demand to the intensity of the impact force of the cavity by rigid extension device, this intensity is to set in advance
It sets, for example, the intensity by the way that impact force is arranged so that the micro-fluidic chip 4 is often primary by impact, in the cavity
One drop of liquid drippage, before implementation, can precalculate the volume and quality of the drop of liquid that micro-fluidic chip 4 often drips,
Due to the quantity that rigid extension device often impacts the drop that micro-fluidic chip 4 is primary, and micro-fluidic chip 4 drips be it is known, therefore
It can be accurately controlled the drippage of the micro-fluidic chip 4 by controlling the number that rigid extension device impacts micro-fluidic chip 4
The amount of liquid；
The micro-fluidic chip 4 can abandon suction nozzle to draw, store and spray the liquid of micro volume, institute for serving as
Stating has cavity and the deformable films covered on cavity, the micro- spray being connected with the cavity by microchannel on micro-fluidic chip
Hole can carry out super-hydrophobic processing at the micro-spraying hole, so that preventing liquid adhesive when sucking liquid by the micro-spraying hole
At micro-spraying hole, the cavity is used to store the liquid of sucking micro-fluidic chip 4, which is used to serve as the spray of dynamic pipettor
Mouthful, it is connected by microchannel between the micro-spraying hole and cavity, the liquid in cavity is not rushed in micro-fluidic chip 4 in order to prevent
It is dripped in the case of hitting, the setting of the micro-spraying hole meets condition：So that liquid has latching characteristics in micro-fluidic chip, and
And the volume of low single drop can be limited by the size of the microchannel and micro-spraying hole, for example, can will be single
The volumetric constraint of drop is within 0.1 nanoliter to 10 nanoliters.Institute's displacement drive 3 is tapped micro- by the extension device connected thereon
Fluidic chip makes the liquid in cavity be sprayed from the spout with drop form；It is provided in the micro-fluidic chip for storing up
The cavity of liquid storage body；
The control system 5 can be wrapped for controlling micro air pump, solenoid valve and the working condition of displacement driver
Controller 510, display module 520 and power supply module 530 are included, the first interface of the controller 510 is connected with solenoid valve, described
The second interface of controller is connected with institute displacement drive, and controller 510 is used to receive the input of parameter, which can be
It is input by user, the work shape of micro air pump 1, solenoid valve 2 and displacement driver 3 described in the state modulator according to the input
State, for example, the control signal that control signal, control micro air pump 1 that output electric control solenoid valve 2 powers on start, command displacement
The control signal etc. that driver 3 starts；The display module can assist user's input parameter, user that can pass through the display mould
Block shows the input parameter of user；The power supply module is used for the electricity consumption power elements in electric pipettor.The driving electricity
Road includes air pump motor driving circuit, driving circuit for electromagnetic valve and displacement driver driving circuit, for responding the controller
Control signal to be driven to micro air pump 1, solenoid valve 2 and displacement driver 3.The control system 5 can also include
Keyboard is inputted, it can also be mechanical keyboard that the input keyboard, which can be touch screen type keyboard, when the input keyboard is to touch
When screen formula keyboard, the input keyboard can be used as the display module, and user can be defeated by the input keyboard
Enter the parameter of setting, the parameter of the input may include：The rotating speed of 1 motor of micro air pump, solenoid valve 2 power on duration, position
It moves driver 3 and impacts the number of the micro-fluidic chip, frequency etc., the premise known to the micro-fluidic chip pipeline flow resistance
Under, the rotating speed realization sucking liquid bulk of micro-fluidic chip 4 for powering on duration, micro air pump motor of the solenoid valve 2 can be passed through
Long-pending control, certainly, if in the case that the rotating speed of micro air pump motor is also fixed value, can directly pass through solenoid valve 2
It powers on duration and realizes that micro-fluidic chip 4 sucks the control of liquid volume, for example, the fixing fabric structure of liquid will be sucked at 0.5 microlitre
To between sub- milliliter.Certainly, user can also input micro-fluidic chip dropping liquid duration and this need to control micro-fluidic chip
The total volume of liquid is discharged, the controller 510 calculates automatically according to the volume of the duration of dropping liquid, total volume and each drop
Displacement driver 3 impacts the number and frequency of the micro-fluidic chip.
By technical solution disclosed in the above embodiments of the present application as it can be seen that the electric pipettor is formed using micro air pump
Negative pressure is drawn liquid into as the power for drawing liquid in the cavity of micro-fluidic chip, can pass through the switch of control solenoid valve
Time and the uptake that control liquid may be implemented using the motor speed for controlling micro air pump are controlled, after drawing liquid
The micro-fluidic chip is impacted using displacement driver, micro-fluidic core is discharged in the liquid in chip in the form of uniform drop
Piece, also, since the liquid volume of each drop is substantially coincident, it can be micro- by controlling electromagnetic driver impact
The number of fluidic chip controls the amount of droplets of drippage and the amount of the drop always dripped, it can be seen that, it is provided by the present application
Electric pipettor has higher precision.
In technical solution disclosed in the embodiment of the present application, due to the miniflow of application various sizes of microchannel and micro-spraying hole
The volume for the drop that control chip 4 drips every time is different, therefore, in order to meet different dropping liquid demands, the above-mentioned reality of the application
It applies in technical solution disclosed in example, the micro-fluidic chip 4 is removably mounted in be inserted for the pipettor of the shell
On head, the micro air pump 1, solenoid valve 2, displacement driver 3 and the installation of control system 5 are on the housing, wherein for side
Just display module described in user's input parameter and input keyboard are arranged in the outer surface of shell, at this point, user can be according to difference
Experiment demand the micro-fluidic chip 4 of different size is installed on the housing, at this point, user can also be by the micro-fluidic chip
The volume of the drop of 4 corresponding drippages inputs to the controller by the input keyboard；
In technical solution disclosed in another embodiment of the application, the miniflow is impacted in order to facilitate institute's displacement drive
Chip is controlled, so that the micro-fluidic chip generates drop of uniform size, a circular cylindrical cavity is provided on the microchannel
Structure, the circular cylindrical cavity structure are used to bear the impact of the rigid extension device, i.e. the rigidity of institute's displacement drive is prolonged
Long part is by impacting the circular cylindrical cavity, so that the micro-fluidic chip generates evenly sized drop.
In technical solution disclosed in the embodiment of the present application, the electric pipettor can be single-pass configuration, can also
For multi-channel structure.
When it is single-pass configuration, one group of micro air pump, solenoid valve, displacement driver and micro-fluidic chip correspond to one
Control system, at this point it is possible to be set to portable construction, referring to Fig. 3, in Fig. 3, the display screen in above-described embodiment
The display module of introduction is connected, and the shell 6 of the electric pipettor is tapering type strip structure, and the micro-fluidic chip setting exists
At the necking of the shell.
The occupation mode of the electric pipettor of the single-pass configuration is as shown in Figure 3：
The first step：As shown in Fig. 3 1., first micro-fluidic chip 4 is mounted on the plug of electric pipettor shell, then
According to demand by inputting keyboard Designing relevant parameter, the volume and displacement driver work such as drawn liquid volume, drip liquid
Working frequency time etc..
Second step：As shown in Fig. 3 2., the micro-spraying hole of 4 end of micro-fluidic chip is immersed in the liquid to be drawn,
Then the corresponding button drawn on the input keyboard is pressed, control system can send out control corresponding with parameter is previously set at this time
Signal processed, i.e., first send out pwm signal, so that the micro air pump is operated in set operating mode, (can be after the delay adjustments time
Several milliseconds), to the high level signal of one setting duration of solenoid valve so that the solenoid valve starts under the action of high level,
The setting duration is to control controller according to the negative pressure that absorption liquid volume and the micro air pump are provided set by user
What size was calculated.After solenoid valve powers on opening, the connected pipe end of micro-fluidic chip is connected to the micro air pump, in gas
It pumps under the suction function formed, liquid is inhaled into from the end micro-spraying hole of micro-fluidic chip in micro-fluidic chip, the pipettor
Will as the step in Fig. 3 3. shown in draw setting volume of liquid.After high level signal, solenoid valve power-off is micro-fluidic
The connected pipe end of chip is connected with air, and it is atmospheric pressure to make the pressure of the liquid storage area of micro-fluidic chip, in order to described micro-
Drop can be smoothly discharged in printing in fluidic chip 4, and due to the scale effect of micro-spraying hole in the micro-fluidic chip 4,
Liquid in micro-fluidic chip has latching characteristics, i.e., in the case of only by its own gravity, the liquid in micro-fluidic chip is not
It can flow out.
Third walks：As shown in Fig. 3 4., the distribution liquid button on input keyboard is pressed, control system can drive to displacement
Dynamic device sends out a square-wave signal for having quantification and frequency, and the quantity of the square-wave signal has with set distribution liquid volume
It closes, frequency is related with the set time.Micro-fluidic chip 4 can drop out designated volume under the continuous percussion of displacement driver
Liquid.
When the electric pipettor is multi-channel structure, the principle of multichannel electric pipettor and single pass electric moving
Liquid device is identical, mainly has Fig. 4-three kinds of embodiments shown in fig. 6, the arrangement mode in each embodiment channel can be single
Arrangement, can also be matrix arrangement.
Referring to Fig. 4, the electric pipettor may include n groups micro air pump, solenoid valve, displacement driver and micro-fluidic core
Piece, the n are the natural number not less than 2；The n groups micro air pump, solenoid valve, displacement driver and micro-fluidic chip share one
Set control system.In Fig. 4, the structure in each channel is identical as single channel, i.e., each channel execution unit is by a miniature gas
Pump, a solenoid valve, a displacement driver and a micro-fluidic chip are constituted.The control signal in each channel is control system point
It does not provide, i.e., user the operating mode in the channel can be arranged by the input keyboard according to the demand in different channels.It is such
The each channel of multichannel electric pipettor is independent, and each channel can draw different volumes liquid according to demand, also may be used
To distribute different volumes liquid according to demand.By the input keyboard be arranged, it is also possible that each channel can simultaneously work
Work can also independently work, naturally it is also possible to partly work at the same time.
Referring to Fig. 5, the electric pipettor may include n groups displacement driver, micro-fluidic chip and solenoid valve, the n
For the natural number not less than 2；The n groups displacement driver, micro-fluidic chip and solenoid valve share one group of micro air pump and control
System.All channels share an air pump, and the control signal in each channel is provided respectively by control system.Implementation shown in Fig. 5
In example, when drawing liquid and distribution liquid, its each channel is also independent from each other, and such multichannel electric pipettor is every
A channel is all independent, and each channel can draw different volumes liquid according to demand, can also distribute difference according to demand
Volume of liquid.Each channel, which can work at the same time, independently to work, and can also partly work at the same time.But due to be by
Each channel shares air pump as the power source for drawing liquid, when liquid is drawn in especially multiple channels simultaneously, the load of air pump
With regard to bigger, this requires air pumps can bear the load of bigger, and the power of air pump will increased accordingly, but reduce
The volume of more electric pipettor devices.
Shown in Fig. 6, the electric pipettor may include n groups displacement driver, micro-fluidic chip, and the n is not less than 2
Natural number；The n groups displacement driver and micro-fluidic chip share one group of micro air pump, solenoid valve and control system.In Fig. 6
Shown in embodiment, all channels share a micro air pump and a solenoid valve, and the control signal in each channel is by control
System provides respectively.Such multichannel electric pipettor, when drawing liquid, each channel is to draw same volume hydrops simultaneously
Body, but when distributing liquid, each channel is independent from each other, and can both be distributed identical or different volume of liquid simultaneously, also may be used
Identical or different volume of liquid is distributed with different time.And the volume of entire electric pipettor can also further decrease.
Further, in order to reduce electric pipettor can cost, in technical solution disclosed in the embodiment of the present application, institute
It states micro-fluidic chip to design based on micro-fluidic principle, including three-decker：The elastic layer on upper layer, intermediate pipe layers and lower layer
High molecular material may be used in supporting layer, elastic layer and pipe layers, and high molecular material or glass, macromolecule may be used in supporting layer
Material is, for example, PDMS (dimethyl silicone polymer).
Further, in the electric pipettor, the concrete type of institute's displacement drive can be according to user demand certainly
Row setting, for example, institute's displacement drive is electromagnetic driver or piezoelectric actuator.The power supply type of the electric pipettor
It can voluntarily be selected according to user demand, such as can be built-in power or external power supply.
It can be seen from the above technical proposal that the invention has the advantages that：
(1), the single droplet size smaller that the present invention generates, the typical volume of drop is between 0.1 nanoliter to 10 nanoliters.
Single droplet size CV values are less than 3.4%, and when carrying out the liquid sample-adding of sub-micro liter volume, average effect can further decrease at random
Error, therefore, using the precision higher of the liquid feeding of the electric pipettor of this programme offer.
(2), micro-fluidic chip of the present invention is made of high molecular material, at low cost and replaceable.It is different from other shiftings
Liquid device, the micro-fluidic chip is detachable, without cleaning, without cross contamination.And it is done at its end micro-spraying hole super-hydrophobic
Processing effectively prevents the liquid adhesive at the micro-spraying hole of micro-fluidic chip end when drawing liquid with distribution liquid.
(3), the present invention realizes absorption and distribution liquid automation mechanized operation, and easy to operate, precision is high.
(4), the present invention controls liquid assimilating volume using controller control solenoid valve switching time, easy to operate and easy
In realization.
(5), apparatus of the present invention it is simple, it is small, be easily designed as hand-held or portable desktop desk-top instrument.
Each embodiment is described by the way of progressive in this specification, the highlights of each of the examples are with other
The difference of embodiment, just to refer each other for identical similar portion between each embodiment.For device disclosed in embodiment
For, since it is corresponded to the methods disclosed in the examples, so description is fairly simple, related place is said referring to method part
It is bright.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest range caused.
Claims (10)
1. a kind of electric pipettor, which is characterized in that including：
Micro air pump, solenoid valve, displacement driver, micro-fluidic chip and control system；
The micro air pump provides power for generating negative pressure to draw liquid；
The solenoid valve, the pipeline that control is connected with micro-fluidic chip when powering on are connected to micro air pump, control when power is off
The pipeline and atmosphere being connected with micro-fluidic chip；
Institute's displacement drive is used at work, by giving micro-fluidic core along the non-telescoping rigid extension device of the direction of motion
Cavity in piece provides an impact force so that the micro-fluidic chip drips drop；
The micro-fluidic chip can abandon suction nozzle to draw, store and spray liquid for serving as；
The control system, for controlling micro air pump, solenoid valve and the working condition of displacement driver；
The cavity for storing liquid and can be tapped is provided in the micro-fluidic chip；
Deformable films are covered on cavity；
The micro-spraying hole of microchannel and cavity perforation is provided on the micro-fluidic chip, the setting of the micro-spraying hole is full
Sufficient condition：So that liquid has latching characteristics in micro-fluidic chip.
2. electric pipettor according to claim 1, which is characterized in that the control system includes：
Controller and input keyboard；
The first interface of the controller is connected with solenoid valve, to export the control for controlling electromagnetic valve work state to solenoid valve
Signal processed；
The second interface of the controller is connected with institute displacement drive, for institute's displacement drive output for controlling
The rigidity extension device impacts the control signal of the deformable films；
The input keyboard and the controller, control signal duration, frequency and number for the controller output to be arranged.
3. electric pipettor according to claim 1, which is characterized in that be provided with a circular cylindrical cavity on the microchannel
Structure, the circular cylindrical cavity structure are used to bear the impact of the rigid extension device.
4. electric pipettor according to claim 1, which is characterized in that institute's displacement drive is electromagnetic driver or pressure
Electric drive.
5. electric pipettor according to claim 1, which is characterized in that the power supply of the electric pipettor is built-in power
Or external power supply.
6. electric pipettor according to claim 1, which is characterized in that driven including n groups micro air pump, solenoid valve, displacement
Dynamic device and micro-fluidic chip, the n are the natural number not less than 2；
The n groups micro air pump, solenoid valve, displacement driver and micro-fluidic chip share a set control system.
7. electric pipettor according to claim 1, which is characterized in that including n groups displacement driver, micro-fluidic chip,
The n is the natural number not less than 2；
The n groups displacement driver and micro-fluidic chip share one group of micro air pump, solenoid valve and control system.
8. electric pipettor according to claim 1, which is characterized in that the electric pipettor includes n group displacement drives
Device, micro-fluidic chip and solenoid valve, the n are the natural number not less than 2；The n groups displacement driver, micro-fluidic chip and electricity
Magnet valve shares one group of micro air pump and control system.
9. electric pipettor according to claim 2, which is characterized in that the micro air pump, solenoid valve, displacement driver
It is mounted in electric pipettor shell with controller, the input keyboard is arranged in the surface of the electric pipettor shell, institute
State the end point that micro-fluidic chip is removably mounted in the shell.
10. electric pipettor according to claim 9, which is characterized in that the shell is tapering type strip structure, described
Micro-fluidic chip is arranged at the necking of the shell.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810516532.9A CN108479874A (en) | 2018-05-25 | 2018-05-25 | A kind of electric pipettor |
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| CN201810516532.9A CN108479874A (en) | 2018-05-25 | 2018-05-25 | A kind of electric pipettor |
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Family
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| CN110052298A (en) * | 2019-05-09 | 2019-07-26 | 中国计量大学 | A method of vibration pipeline control micro-fluidic chip generates microlayer model |
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| CN113711056A (en) * | 2019-04-18 | 2021-11-26 | 美国西门子医学诊断股份有限公司 | Integrated microfluidic device with pipettor adaptation |
| CN115106143A (en) * | 2022-06-29 | 2022-09-27 | 合肥瀚海星点生物科技有限公司 | High-precision electric micro-liquid pipettor |
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