CN108469477A - A kind of assay method of Benzene in Beverages carbamide compound - Google Patents
A kind of assay method of Benzene in Beverages carbamide compound Download PDFInfo
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- CN108469477A CN108469477A CN201810163576.8A CN201810163576A CN108469477A CN 108469477 A CN108469477 A CN 108469477A CN 201810163576 A CN201810163576 A CN 201810163576A CN 108469477 A CN108469477 A CN 108469477A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/08—Preparation using an enricher
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N2030/042—Standards
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
Abstract
A kind of assay method of Benzene in Beverages carbamide compound disclosed by the invention, including step：S1. the configuration of standard solution；S2. on-line solid phase extraction and separation；S3. qualitative and quantitative analysis.The present invention uses on-line solid phase extraction/high performance liquid chromatography, integrates Solid Phase Extraction and qualitative and quantitative analysis, under the conditions of without cumbersome manual operation, completes quick, the Accurate Determining of Benzene in Beverages carbamide compound.
Description
Technical field
The invention belongs to chemical analysis technology field, more particularly to a kind of assay method of Benzene in Beverages carbamide compound.
Background technology
Phenylurea compound is a kind of widely used herbicide, can persistently be existed in the environment, and is generated to human health
Harm.It is mainly mass spectrometer and liquid chromatography to measure the common method of phenylurea compound at present.Due to phenylurea compound
Concentration in the sample is often relatively low, and itself is easily thermally decomposed, polarity is big, often need to be in conjunction with extraction, concentration using mass spectrometer
And derivative；To meet detection limit requirement and reducing matrix interference, solid phase extraction need to be often first passed through using high performance liquid chromatography sample
It takes, for example EPA532 combines offline Solid Phase Extraction and high performance liquid chromatography, using phosphate buffer and acetonitrile as mobile phase,
Measure nine kinds of phenylurea compounds in water body.To sum up, the method generally existing for measuring phenylurea compound at present is cumbersome, time-consuming
The shortcomings of consumption of long and organic solvent is big.
The present inventor is using double gradient high performance liquid chromatographs, it is proposed that a kind of on-line solid phase extraction/high performance liquid chromatography
The method that method measures Benzene in Beverages carbamide compound.
Invention content
It, can will be after beverage filter directly the purpose of the present invention is to provide a kind of assay method of Benzene in Beverages carbamide compound
Sample introduction, the Solid Phase Extraction of this method collection sample and is detected on one, avoids cumbersome manual operation and human error, has letter
Just, quickly, accurately and save organic solvent the characteristics of.
To achieve the goals above, technical scheme is as follows：
A kind of assay method of Benzene in Beverages carbamide compound, includes the following steps：
S1. the preparation of standard solution
It takes 10 μ L, 100 μ g/mL phenylureas standard mixed solution M-532 in 10 mL volumetric flasks, with methanol constant volume to scale, shakes
It is even, obtain a concentration of 100 μ g/L phenylurea standard reserving solutions；Take 10 μ L, 100 μ g/mL phenylurea phenylurea tracer standard solution M-
532SS, with methanol constant volume to scale, shakes up in 10 mL volumetric flasks, obtains the tracer storing solution of a concentration of 500 μ g/L,
A certain amount of phenylurea standard reserving solution and tracer storing solution are taken, is 1 with volume ratio：49 acetonitrile and water dilution, obtains phenylurea
Compound standard curve concentration：0.5 μ g/L, 1 μ g/L, 2 μ g/L, 5 μ g/L, 10 μ g/L, 20 μ g/L, 50 μ g/L and
100 μg/L；Phenylurea concentration of tracer in each calibration curve solution is 20 μ g/L；
S2. on-line solid phase extraction and separation
Using double gradient high performance liquid chromatographs to sample solution, sample mark-on solution and phenylurea compound standard curve solution
Machine testing is carried out, the sample solution uses green tea beverage, sample mark-on solution to use and add a certain amount of concentration phenylurea
The green tea beverage of object mixed standard solution is closed, double gradient high performance liquid chromatographs contain double gradient pump, autosampler, column temperatures
Case, six-way valve, SPE columns, analytical column and diode array detector；Its on-line solid phase extraction and separation process are as follows：
0-5.8 min, for six-way valve at 1-2, enrichment pump completes sample introduction, target compound on SPE columns by autosampler
Enrichment, analysis pump is in equilibrium analysis column；
5.8-7.8 min, for six-way valve at 6-1, SPE columns are introduced into analysis flow path, and target compound is eluted from SPE columns
And it flows into analytical column and is initially separated；
7.8-20 min, six-way valve are brought out analysis flow path in 1-2, SPE columns and are back to original state, and analytical column continues
Isolating target compound is simultaneously back to original state；It is dividing for next sample to be back to the SPE columns of original state and analytical column
Analysis is prepared；
S3. qualitative and quantitative analysis
1）Qualitative analysis：When the retention time of the retention time of target compound in sample and phenylurea compound mixed standard solution
Error spectrogram of target compound and when the consistent spectrogram of standard solution within 5% and in sample, then judge to contain in sample
There is phenylurea compound；
2）Quantitative analysis：Continuously into the mixed standard solution of a concentration of 100 μ g/L of nine sample introductions, target compound reservation is obtained
The relative standard deviation of time and peak area is respectively less than 5%；The standard curve of each target compound chromatogram has good line
Property, and the rate of recovery of method within the scope of 70-130%, then this method is suitable for the measurement of Benzene in Beverages carbamide compound；When a certain
Signal-to-noise ratio >=10 of target compound in the sample, then it is assumed that the target compound has detection in the sample.
Further, the phenylurea compound is grand Thidiazuron, fluometuron, diuron, propanil, Tupersan A, Tupersan B, spy
It is one or more in fourth benzthiazuron, linuron, weeding urea；The phenylurea tracer is telvar and/or carbazole.
Further, the on-line solid phase extraction and separation condition are as follows：
（1）Solid phase extraction system：
SPE columns：SolEx HRP, 12-14 μm, 2.1 × 20 mm
Mobile phase： A：Water, B：Methanol
Eluent gradient：0-4 min, 10-100% B；13 min, 100% B；13.1-20 min, 10% B
Flow velocity： 1.0 mL/min
Sampling volume： 2500 µL；
（2）Analysis system：
Analytical column：C18,3 μm, 3 × 150 mm
Mobile phase：A：20 mM ammonium formate solutions, B：Acetonitrile
Eluent gradient：0-4 min, 35% B；4.1 min, 40% B；7.5-15.8 min, 60% B；16 min35% B
Column temperature：25 °C
Flow velocity：0.6 mL/min
Detection wavelength：245 nm
The Vavle switching time：0 min, 1_2；5.8 min, 6_1；7.8 min, 1_2.
After adopting the above scheme, the present invention has the beneficial effect that：The present invention is straight suitable for the liquid samples such as beverage or water body
Sample detection after filtering is taken over, enrichment and qualitative and quantitative analysis can be completed on liquid chromatograph simultaneously, be not only not necessarily to cumbersome people
For pre-treatment, and organic solvent consumption less, take it is short, have the characteristics that it is easy, quickly, accurate and saving organic solvent, can
It is applied to the phenylurea compound of liquid sample or even the detection of other trace compounds in detection food and environment.
The characteristics of saving solvent, the phenylurea compound residual that can be applied in detection food and environment.
Below in conjunction with the drawings and specific embodiments, the invention will be further described.
Description of the drawings
Fig. 1 is a kind of general flow chart of the assay method of Benzene in Beverages carbamide compound of the present invention；
Fig. 2 is double gradient high performance liquid chromatograph on-line solid phase extraction model process figures that the present invention uses；
Fig. 3 is phenylurea compound chromatogram；
Fig. 4 is green tea sample (a), green tea sample mark-on (b) and phenylurea compound mixed standard solution (c) chromatogram.
Specific implementation mode
As shown in Figure 1, a kind of assay method for Benzene in Beverages carbamide compound that the present embodiment discloses, includes the following steps：
S1. the preparation of standard solution
It takes 10 μ L, 100 μ g/mL phenylureas standard mixed solution M-532 in 10 mL volumetric flasks, with methanol constant volume to scale, shakes
It is even, obtain a concentration of 100 μ g/L phenylurea standard reserving solutions；Take 10 μ L, 100 μ g/mL phenylurea phenylurea tracer standard solution M-
532SS, with methanol constant volume to scale, shakes up in 10 mL volumetric flasks, obtains the tracer storing solution of a concentration of 500 μ g/L,
A certain amount of phenylurea standard reserving solution and tracer storing solution are taken, is 1 with volume ratio：49 acetonitrile and water dilution, obtains phenylurea
Compound standard curve concentration：0.5 μ g/L, 1 μ g/L, 2 μ g/L, 5 μ g/L, 10 μ g/L, 20 μ g/L, 50 μ g/L and
100 μg/L；Phenylurea concentration of tracer in each calibration curve solution is 20 μ g/L；
The phenylurea compound that can measure of the present invention includes grand Thidiazuron, fluometuron, diuron, propanil, Tupersan A, Tupersan
B, terbufos benzthiazuron, linuron, weeding urea；Above-mentioned phenylurea tracer is telvar and/or carbazole.
S2. on-line solid phase extraction and separation
Using double gradient high performance liquid chromatographs to sample solution, sample mark-on solution and phenylurea compound standard curve solution
Machine testing is carried out, the sample solution uses green tea beverage, sample mark-on solution to use and add a certain amount of concentration phenylurea
The bottled green tea beverage of object mixed standard solution is closed, a certain amount of concentration refers to the phenylurea compound mixed standard solution concentration being added
Concentration identical with the phenylurea compound mixed standard solution of upper machine testing；
As shown in Fig. 2, double gradient high performance liquid chromatographs contain double gradient pumps 1（Including enrichment pump and analysis pump）, autosampler
2, column oven（It is not shown in figure）, six-way valve 3, SPE columns 4（Solid-phase extraction column）, analytical column 5 and diode array detector 6；This
The on-line solid phase extraction and separating experiment condition of embodiment are as follows：
（1）Solid phase extraction system：
SPE columns：SolEx HRP, 12-14 μm, 2.1 × 20 mm
Mobile phase： A：Water, B：Methanol
Eluent gradient：0-4 min, 10-100% B；13 min, 100% B；13.1-20 min, 10% B
Flow velocity： 1.0 mL/min
Sampling volume： 2500 µL；
（2）Analysis system：
Analytical column：C18,3 μm, 3 × 150 mm
Mobile phase：A：20 mM ammonium formate solutions, B：Acetonitrile
Eluent gradient：0-4 min, 35% B；4.1 min, 40% B；7.5-15.8 min, 60% B；16 min35% B
Column temperature：25 °C
Flow velocity：0.6 mL/min
Detection wavelength：245 nm
The Vavle switching time：0 min, 1_2；5.8 min, 6_1；7.8 min, 1_2.
Based on above-mentioned condition, this example on-line solid phase extraction and separation process are as follows：
0-5.8 min, for six-way valve at 1-2, enrichment pump completes sample introduction, target compound in SPE columns 4 by autosampler 2
Upper enrichment, analysis pump is in equilibrium analysis column 5；
5.8-7.8 min, six-way valve is at 6-1, and SPE columns 4 are introduced into analysis flow path, under target compound is eluted from SPE columns 4
Carry out and flow into analytical column 5 and is initially separated；
7.8-20 min, at 1-2, SPE columns 4 are brought out analysis flow path and are back to original state six-way valve, analytical column 5 after
Continuous isolating target compound is simultaneously back to original state；It is next sample to be back to the SPE columns 4 of original state and analytical column 5
Analysis prepare；
S3. qualitative and quantitative analysis
1）Qualitative analysis：In conjunction with Fig. 3, when the retention time of target compound in sample and phenylurea compound mixed standard solution
Retention time error spectrogram of target compound and when the consistent spectrogram of standard solution within 5% and in sample, then judge
Contain phenylurea compound in sample；
2）Quantitative analysis：Continuously into the mixed standard solution of a concentration of 100 μ g/L of nine sample introductions, target compound reservation is obtained
The relative standard deviation of time and peak area is respectively less than 5%；The standard curve of each target compound chromatogram has good line
Property, and the rate of recovery of method within the scope of 70-130%, then this method is suitable for the measurement of Benzene in Beverages carbamide compound；When a certain
Signal-to-noise ratio >=10 of target compound in the sample, then it is assumed that the target compound has detection in the sample.
In conjunction with Fig. 4, in figure：
1- tebuthiurons, 2- Thidiazurons, 3- telvars (tracer, 20 μ g/L), 4- fluometuron, 5- diuron, 6- propanil,
7- Tupersans A, 8- Tupersan B, 9- linuron, 10- carbazoles (tracer, 20 μ g/L), 11- diflubenzurons；
Green tea sample spiked levels and phenylurea compound mixed standard solution concentration are 2.5 μ g/L, and wherein concentration of tracer is
20 μ g/L, comparison green tea sample (a), green tea sample mark-on (b) and phenylurea compound mixed standard solution (c) chromatogram are found
There is the phenylureas such as terbufos benzthiazuron, linuron and diflubenzuron compound to be detected in green tea sample.
Specific embodiments of the present invention are these are only, not to the restriction of protection scope of the present invention.
The equivalent variations that all mentalities of designing according to this case are done, each fall within the protection domain of this case.
Claims (3)
1. a kind of assay method of Benzene in Beverages carbamide compound, which is characterized in that include the following steps：
S1. the preparation of standard solution
It takes 10 μ L, 100 μ g/mL phenylureas standard mixed solution M-532 in 10 mL volumetric flasks, with methanol constant volume to scale, shakes
It is even, obtain a concentration of 100 μ g/L phenylurea standard reserving solutions；Take 10 μ L, 100 μ g/mL phenylurea phenylurea tracer standard solution M-
532SS, with methanol constant volume to scale, shakes up in 10 mL volumetric flasks, obtains the tracer storing solution of a concentration of 500 μ g/L,
A certain amount of phenylurea standard reserving solution and tracer storing solution are taken, is 1 with volume ratio：49 acetonitrile and water dilution, obtains phenylurea
Compound standard curve concentration：0.5 μ g/L, 1 μ g/L, 2 μ g/L, 5 μ g/L, 10 μ g/L, 20 μ g/L, 50 μ g/L and
100 μg/L；Phenylurea concentration of tracer in each calibration curve solution is 20 μ g/L；
S2. on-line solid phase extraction and separation
Using double gradient high performance liquid chromatographs to sample solution, sample mark-on solution and phenylurea compound standard curve solution
Machine testing is carried out, the sample solution uses green tea beverage, sample mark-on solution to use and add a certain amount of concentration phenylurea
The green tea beverage of object mixed standard solution is closed, double gradient high performance liquid chromatographs contain double gradient pump, autosampler, column temperatures
Case, six-way valve, SPE columns, analytical column and diode array detector；Its on-line solid phase extraction and separation process are as follows：
0-5.8 min, for six-way valve at 1-2, enrichment pump completes sample introduction, target compound on SPE columns by autosampler
Enrichment, analysis pump is in equilibrium analysis column；
5.8-7.8 min, for six-way valve at 6-1, SPE columns are introduced into analysis flow path, and target compound is eluted from SPE columns
And it flows into analytical column and is initially separated；
7.8-20 min, six-way valve are brought out analysis flow path in 1-2, SPE columns and are back to original state, and analytical column continues
Isolating target compound is simultaneously back to original state；It is dividing for next sample to be back to the SPE columns of original state and analytical column
Analysis is prepared；
S3. qualitative and quantitative analysis
1）Qualitative analysis：When the retention time of the retention time of target compound in sample and phenylurea compound mixed standard solution
Error spectrogram of target compound and when the consistent spectrogram of standard solution within 5% and in sample, then judge to contain in sample
There is phenylurea compound；
2）Quantitative analysis：Continuously into the mixed standard solution of a concentration of 100 μ g/L of nine sample introductions, target compound reservation is obtained
The relative standard deviation of time and peak area is respectively less than 5%；The standard curve of each target compound chromatogram has good line
Property, and the rate of recovery of method within the scope of 70-130%, then this method is suitable for the measurement of Benzene in Beverages carbamide compound；When a certain
Signal-to-noise ratio >=10 of target compound in the sample, then it is assumed that the target compound has detection in the sample.
2. a kind of assay method of Benzene in Beverages carbamide compound as described in claim 1, it is characterised in that：The phenylurea chemical combination
Object is in grand Thidiazuron, fluometuron, diuron, propanil, Tupersan A, Tupersan B, terbufos benzthiazuron, linuron, weeding urea
It is one or more；The phenylurea tracer is telvar and/or carbazole.
3. a kind of assay method of Benzene in Beverages carbamide compound as described in claim 1, it is characterised in that：The online solid phase
Extraction and separation condition are as follows：
（1）Solid phase extraction system：
SPE columns：SolEx HRP, 12-14 μm, 2.1 × 20 mm
Mobile phase： A：Water, B：Methanol
Eluent gradient：0-4 min, 10-100% B；13 min, 100% B；13.1-20 min, 10% B
Flow velocity： 1.0 mL/min
Sampling volume： 2500 µL；
（2）Analysis system：
Analytical column：C18,3 μm, 3 × 150 mm
Mobile phase：A：20 mM ammonium formate solutions, B：Acetonitrile
Eluent gradient：0-4 min, 35% B；4.1 min, 40% B；7.5-15.8 min, 60% B；16 min35% B
Column temperature：25 °C
Flow velocity：0.6 mL/min
Detection wavelength：245 nm
The Vavle switching time：0 min, 1_2；5.8 min, 6_1；7.8 min, 1_2.
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| CN201810163576.8A Pending CN108469477A (en) | 2018-02-02 | 2018-02-27 | A kind of assay method of Benzene in Beverages carbamide compound |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113267579A (en) * | 2021-05-18 | 2021-08-17 | 中南民族大学 | Method for monitoring blood concentration of risperidone |
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2018
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| CN105467019A (en) * | 2014-08-18 | 2016-04-06 | 中粮营养健康研究院有限公司 | Determination method of vitamin A and vitamin E and corresponding on-line solid phase extraction analysis system |
| CN105203652A (en) * | 2015-08-21 | 2015-12-30 | 河南省农业科学院农业质量标准与检测技术研究所 | Method for detecting regulating-agent thidiazuron residues and regulating-agent diuron residues |
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| 滕曼 等: "在线固相萃取-高效液相色谱法测定水中的苯脲化合物", 《环境科学》 * |
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