CN108452298A - A kind of technique producing yellow fever attenuated live vaccine with SPF chick-embryo cells - Google Patents
A kind of technique producing yellow fever attenuated live vaccine with SPF chick-embryo cells Download PDFInfo
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- CN108452298A CN108452298A CN201710085260.7A CN201710085260A CN108452298A CN 108452298 A CN108452298 A CN 108452298A CN 201710085260 A CN201710085260 A CN 201710085260A CN 108452298 A CN108452298 A CN 108452298A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
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- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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- A61K2039/5254—Virus avirulent or attenuated
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
- C12N2770/00011—Details
- C12N2770/24011—Flaviviridae
- C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
- C12N2770/24151—Methods of production or purification of viral material
Abstract
The invention belongs to field of biological product, and in particular to a kind of technique producing yellow fever attenuated live vaccine with SPF chick-embryo cells.The present invention uses the 17D attenuated vaccine strains that WHO is provided, through seed culture of viruses, stroma cell of the chick-embryo cell that inoculating cell factory cultivates as Virus culture is made in chick-embryo cell adaptation of virus; virus liquid is collected after culture; using degerming, gelatin-free protective agent is added, then freeze-drying prepares finished product vaccine.Vaccine potency index accords with WHO regulations, and other quality control standards (QCS)s meet 2015 editions《Chinese Pharmacopoeia》Third portion requires.It is infected for preventing yellow fever.
Description
Technical field
The invention belongs to biotechnologies, and in particular to a kind of to produce yellow fever attenuated live vaccine with SPF chick-embryo cells
Technique.
Background technology：
Yellow fever is her mosquito-borne serious thermophilic visceral and Neural invasion viral infectious, and case fatality rate is very high, right and wrong
The public health problem that continent and many countries of South America pay special attention to.In Africa, 5.08 hundred million national population structures of the disease pair 33
At seriously threatening, African sub- the Sahara poverty-stricken area is mostly occurred in.It is at least reported every year with Endemic Areas such as Africa in South America
Case is more than 200,000, and death is more than 30,000.Though China is not at epidemic-stricken area, annual past as China's foreign economic opens
More and more, the biography of yellow fever come the personnel in Africa, South America torrid areas (labor service is outgoing, public affair accesses, tourism is on home leave)
It is very wide to broadcast medium-yellow-fever mosquito distribution, it is increasing to flavivirus potential hazard.Due to not treated targetedly to yellow fever
Measure, vaccine inoculation are the only effective prevention methods, thus to people going abroad's immunoprophylaxis prevention be it is extremely necessary, simultaneously
Also it to maintain vigilance to flavivirus bringing into through returned personnel, carry out the preparation of reply.
The current domestic production yellow fever vaccine in China.Annual about 300,000 person-portions are mainly used for people going abroad's inoculation.
These vaccines are inoculated with using SPF chick embryo yolk sacs, and chicken embryo tissue culture is ground to wait process that the crude vaccine of full embryo is made.By
More in impurity protein, there is insecurity, it is necessary to be improved.
For guarantor's vaccine safety, to vaccine, using production stroma cell, there are strict requirements for national Bureau of Drugs Supervision.Two
Times body cell is because coming from human normal tissue, by stringent calibrating, it was demonstrated that be a kind of safest cell, but because source has
Limit, cannot meet mass production needs.VERO cells come from
African green monkey kidney transformed cells have potential oncogenicity, although WHO is allowed for production of vaccine, have stringent
Generation limitation, country also formulated stringent residual DNA control standard.And general primary zooblast, there is external source because
Son pollution.For only SPF chick-embryo cells because material is easy to get, exogenous factor pollution is few to be classified as production of vaccine first choice by national Bureau of Drugs Supervision
Cell.
The present invention discloses a kind of safe yellow fever vaccine production technology, can equally reach existing chicken embryo production yellow fever and subtract
The vaccine potency and protecting effect that virus live vaccine WHO is required.This technique vaccine impurity is few, and side reaction is less than traditional processing technology system
The vaccine made, vaccine safety are more preferable.Production of vaccine efficiency can be greatly improved, reduce cost simultaneously.
Invention content
A kind of production technology with chick-embryo cell yellow fever attenuated live vaccine of the invention.Specially：It is trained using cell factory
Poultry blastocyte is Virus culture stroma cell, and inoculation passes through through the 17D attenuated vaccine strain seeds culture of viruses in chick-embryo cell adaptation of virus
Virus liquid is collected in culture, and finished product vaccine is prepared using freeze-drying after degerming, purifying, addition protective agent.
The present invention relates to a kind of Yellow Fever Vaccine,Live production technology is produced with chick-embryo cell culture 17D strains.Using thin
Chick-embryo cell is cultivated as Virus culture stroma cell by born of the same parents factory, is inoculated with the 17D attenuated vaccine strains through chick-embryo cell adaptation of virus
Seed culture of viruses collects virus liquid by culture, after filtering out bacterium, freeze drying protectant is added, freeze-drying prepares finished product vaccine.
In a preference, the 17D attenuated vaccine strain seeds culture of viruses used in the present invention are what the World Health Organization (WHO) provided
17D-204 strains (NIBS CODE 213-77).Chick-embryo cell is prepared with SPF chicken embryos.Chick-embryo cell seed culture of viruses used in the present invention
For seed culture of viruses of the inventor through chick-embryo cell adaptation of virus.Seed culture of viruses titre presses mouse LD50Method titre is not less than 5.60LD50；Plaque method
Titration is not less than 5.8LgPFU/mL.
In another preference, this vaccine bebcell cultural method is：
1) trypsin digestion and cells concentration 0.25%, pH are 7.4~7.6.
2) chick-embryo cells culture solution is containing 5% newborn bovine serum, 0.3% lactoalbumin hydrolysate, 1%NaHCO3, 2mM paddy ammonia
The MEM culture mediums of amide.
3) viruses culture solutions are the DMEM solution without cow's serum, and 2%~3%NaHCO is added3,
PH7.4~7.6, while adding 0.5% human albumin.
4) for the culture of chick-embryo cells using cell factory as culture carrier, cultivation temperature is 37 ± 0.1.
In another preference, vaccine preparation uses method：
1) viruses point kind method：Using cell factory culture chick-embryo cell.Chick-embryo cell presses 2~8 × 10 after digesting5/
cm2Cell number is inoculated into cell factory, cell culture fluid is added, 37 ± 0.1 DEG C of cultures, inoculated into chick embryo is thin after cell is at single layer
Born of the same parents' seed culture of viruses.
It is trained the cell of single layer, first the culture solution of falling virus removal, by 0.01~0.5 virus inoculations of MOI.After virus inoculation
Cell set 35 DEG C~37 DEG C 4~6 hours, make virus with cell come into full contact with absorption.Then prevent or cure a disease venom, it is slow with phosphate
Fliud flushing (PBS) is rinsed cell surface, is eventually adding the virus-culturing fluids of pH7.4~7.6, is sent into 35 DEG C~37 DEG C thermostatic chambers
It is cultivated.
2) viral miscegenation method is：After postdigestive cell suspension is sufficiently mixed by 0.001~0.1MOI virus inoculations, point
It is filled to cell factory, sets 35 DEG C~37 DEG C cultures.
The culture solution of falling virus removal after culture 48 hours, is rinsed cell surface with phosphate buffer (PBS), finally
Virus-culturing fluid is added, 35 DEG C~37 DEG C cultures are sent into pH7.4~7.6.
In another preference, the two methods virus harvest time be from be inoculated with after cultivate 72~96 hours after receive
Obtain virus.The virus liquid of harvest is again stoste, assay approval through 0.22 μm of aseptic filtration after 1 μm or 0.6 μm of filter membrane clarification
Addition freeze drying protectant carries out freeze-drying and vaccine is made afterwards.
In another preference, the ingredient that this patent vaccine protects freeze-drying shield agent solution is：PH7.4 without gelatin~
7.6DMEM polysaccharide solution.
Description of the drawings
Fig. 1 chick-embryo cell yellow fever attenuated live vaccine process flow charts (miscegenation).
Fig. 2 chick-embryo cell yellow fever attenuated live vaccines process flow chart (point kind).
The flow chart of the vaccine bebcell cultural method of Fig. 3 present invention.
Specific implementation mode
Below in conjunction with specific embodiment, the present invention will be further described.The present embodiment is only used for the description of the invention,
It is not construed as limiting the invention.
17D attenuated vaccine strain seeds culture of viruses used in the present invention are the 17D-204 strains that the World Health Organization (WHO) provides
(NIBS CODE 213-77) (Fig. 3).Chick-embryo cell is prepared with SPF chicken embryos.Chick-embryo cell seed culture of viruses used in the present invention is invention
Seed culture of viruses of the people through chick-embryo cell adaptation of virus.Seed culture of viruses titre presses mouse LD50Method titre is not less than 5.60LD50；Plaque method titrates not
Less than 5.8LgPFU/mL.
This vaccine bebcell cultural method is：
1) trypsin digestion and cells concentration 0.25%, pH are 7.4~7.6.
2) chick-embryo cells culture solution is containing 5% newborn bovine serum, 0.3% lactoalbumin hydrolysate, 1%NaHCO3, 2mM paddy ammonia
The MEM culture mediums of amide.
3) viruses culture solutions are the DMEM solution without cow's serum, and 2%~3%NaHCO is added3,
PH7.4~7.6, while adding 0.5% human albumin.
4) for the culture of chick-embryo cells using cell factory as culture carrier, cultivation temperature is 37 ± 0.1.
Vaccine preparation uses method：
1) viruses point kind method：
Using cell factory culture chick-embryo cell.Chick-embryo cell presses 2~8 × 10 after digesting5/cm2Cell number is inoculated into carefully
Born of the same parents factory, is added cell culture fluid, and 37 ± 0.1 DEG C of cultures wait for that cell is followed by kind of a chick-embryo cell seed culture of viruses at single layer.
It is trained the cell of single layer, first the culture solution of falling virus removal, by 0.01~0.5 virus inoculations of MOI.After virus inoculation
Cell set 35 DEG C~37 DEG C 4~6 hours, make virus with cell come into full contact with absorption.Then prevent or cure a disease venom, it is slow with phosphate
Fliud flushing (PBS) is rinsed cell surface, is eventually adding the virus-culturing fluids of pH7.4~7.6, is sent into 35 DEG C~37 DEG C thermostatic chambers
It is cultivated.
2) viral miscegenation method is：
After postdigestive cell suspension is sufficiently mixed by 0.001~0.1MOI virus inoculations, dispenses to cell factory, set 35
DEG C~37 DEG C of cultures.
The culture solution of falling virus removal after culture 48 hours, is rinsed cell surface with phosphate buffer (PBS), finally
Virus-culturing fluid is added, 35 DEG C~37 DEG C cultures are sent into pH7.4~7.6.
The two methods virus harvest time be after cultivate 72~96 hours from after being inoculated with harvest it is viral.The virus of harvest
Liquid through 0.22 μm of aseptic filtration is again stoste after 1 μm or the clarification of 0.6 μm of filter membrane, be added after assay approval freeze drying protectant into
Vaccine is made in row freeze-drying.
Embodiment 1, chick-embryo cell digestion
Chick-embryo cell comes from domestic SPF chicken embryos, takes the 9-10 day instar chicken embryos of normal development, and chicken is taken out in sterile working method
Embryo removes head and internal organ, is cleaned three times with PBS, is cut into 2-3mm tissue blocks with sterilizing medical operation, is added 0.25%
Trypsin solution digests 20 points of kinds, and centre gently shakes for 10 minutes makes trypsase be come into full contact with tissue block.
When digestion terminates, trypsin solution is removed.Then 4 are blown and beaten by 20,30,50,40 to tissue block with piping and druming suction pipe
Secondary, dynamics is by please be to gradual exacerbation.After having beaten every time, cell culture fluid, as cell suspension is added by per embryo 30ml.
Examples of implementation 2, chick-embryo cell are cultivated on cell factory and virus inoculation
1) virus point kind method is：
Using cell factory culture chick-embryo cell.Chick-embryo cell presses 2~4 × 10 after digesting5/cm2Cell number is inoculated into carefully
Born of the same parents factory, is added cell culture fluid, and 37 ± 0.1 DEG C of cultures wait for that cell is followed by kind of a chick-embryo cell seed culture of viruses at single layer.Specific method：
It is trained the cell of single layer, first the culture solution of falling virus removal, by 0.01~0.5 virus inoculations of MOI.After virus inoculation
Cell set 35 DEG C~37 DEG C 4~6 hours, make virus with cell come into full contact with absorption.Then prevent or cure a disease venom, it is slow with phosphate
Fliud flushing (PBS) is rinsed cell surface, is eventually adding the virus-culturing fluids of pH7.4~7.6, is sent into 35 DEG C~37 DEG C thermostatic chambers
It is cultivated.
Chick-embryo cell yellow fever attenuated live vaccine process flow chart (miscegenation) such as Fig. 1.
2) viral miscegenation method is：
After postdigestive cell suspension is sufficiently mixed by 0.001~0.1MOI and virus, by 3~5 × 105/cm2Packing is extremely
Cell factory sets 35 DEG C~37 DEG C cultures.
The culture solution of falling virus removal after culture 48 hours, is rinsed cell surface with phosphate buffer (PBS), finally
Virus-culturing fluid is added, 35 DEG C~37 DEG C cultures are sent into pH7.4~7.6.
In the above cell factory cell growth medium is added by every layer of 200ml.
Chick-embryo cell yellow fever attenuated live vaccine process flow chart (point kind) such as Fig. 2.
Embodiment 3, virus harvest
Virus inoculation 72 to 96 hours, when pathological development extremely +++~++++Virus can be harvested.
Examples of implementation 4：It is prepared by seed culture of viruses
Viral continuous passage is carried out with above method and prepares the yellow hot vaccine virus seeds of 17D, when titre reaches 5.6lgPFU/
Ml, which is believed that, obtains the 17D Strain that chick-embryo cell adapts to, and it is viral seed to carry out indices assay approval.
The calibrating of seed lot seed culture of viruses
Main seed lot should carry out following comprehensive calibrating：
1) discrimination test
Discrimination test is carried out using plaque method.By viral dilution to 50~100PFU/0.4ml, with yellow fever virus specificity
Immune serum and non-immune serum mixed in equal amounts in 37 ± 1 DEG C of water-baths and 60 minutes, are set 35 ± 1 DEG C and are cultivated 6 days, immune serum
The plaque number of group should be not less than 80% than the slip of non-immune serum group.Serum and cell controls should be set simultaneously, should be cloudy
Property；Virus control virus quantity should be 50~100PFU/0.4ml, should be positive.
2) virus titer
Plaque method can be used or mouse method carries out titration of virus.Main seed lot should use plaque method and mouse LD simultaneously50Method
Carry out titration of virus.
A. plaque method：
It takes seed culture of viruses to carry out 10 times of dilutions, then carries out 4 times and be serially diluted, take 3~4 acceptable diluent degree inoculation Vero cells,
It sets 35 ± 1 DEG C to cultivate 6 days, while setting cell controls, plaque number, virus titer are counted after Plaque Formation.It should be not less than
5.8LgPFU/ml。
B. mouse method：
It takes seed culture of viruses to carry out 10 times to be serially diluted, 10~12g of each dilution poison disease vaccination mouse 6, every intracerebral
0.03ml observes died in 21 days, 3 days and disregards, (animal dead quantity should must not exceed the 20% of experimental animal sum), meter
Calculate LD50.Virus titer should be not less than 5.50lgLD50/ml.
3) sterility test checks in accordance with the law, should meet 2015 editions《Chinese Pharmacopoeia》Regulation.
4) mycoplasma inspection checks in accordance with the law, should meet 2015 editions《Chinese Pharmacopoeia》Regulation.
5) viral exogenous factor inspection checks in accordance with the law, should meet 2015 editions《Chinese Pharmacopoeia》Regulation.
6) exogenous avian leukosis virus detection
Test sample is inoculated with SPF chicken embryos, culture is checked using enzyme-linked immunization after culture, as a result should be negative.
7) exogenous aviadenovirus detection
Test sample is inoculated with SPF Embryo liver cell cultures, detects training with suitable serological method respectively
As a result the I types and type III adenovirus for supporting object should be negative.
8) gene sequencing
Into the viral seed of cell continuous passage adaptation, its gene order is consistent with jungle fowl embryo culture virus seed.
9) monkey body test
Main seed lot carries out monkey body test.2015 editions should be met《Chinese Pharmacopoeia》Regulation.
1)~8 working seed lots should at least carry out) item assay approval.
First three batch vaccinogen liquid of the freshly prepd seed lot for when producing, continuously preparing should carry out viral key gene sequence
Row measure, and measurement result should be consistent with main seed lot.
10) virus seed conservation
Seed lot seed culture of viruses preserves after should being lyophilized in -60 DEG C or less.
Embodiment 5, virus harvest
Virus after harvest removes cell fragment host protein through 1 μm or 0.6 μm of membrane filtration with 0.2 μm of filter membrane degerming,
It is stoste that freeze drying protectant, which is added, and stoste should be placed in -20 DEG C of preservations.After and to freeze-drying sets 2 DEG C~8 DEG C in time after titre qualification
It preserves.
With preparation method of the present invention, vaccine primary quality measure is：
Malicious finished product titre is not less than 4.5IU/ml/ agent
Vaccine immunity dosage：0.5ml/ people
Egg white protein content：≤ 100ng/ agent
Antibiotic residual quantity：≤ 50ng/ agent
Cow's serum residual quantity：≤ 50ng/ agent
Pyrogen：≤ 5EU/ agent
Vaccine pH:7.2-8.0
Moisture is lyophilized：≤ 3%
Thermal stability:37 DEG C place 2 weeks after be not less than 4.5LgPFU/ml/ agent, 37 DEG C place 2 weeks after titre decline do not surpass
Cross 1.0LgPFU/ml.
Sterility test：Meet 2015《Chinese Pharmacopoeia》Third ministerial standard is qualified.
Mycoplasma inspection：Meet 2015《Chinese Pharmacopoeia》Third ministerial standard is qualified.
Abnormal toxicity test：Meet 2015《Chinese Pharmacopoeia》Third ministerial standard is qualified.
Discrimination test：It is qualified.
It should be understood that after reading the above teachings of the present invention, those skilled in the art can make the present invention
Various changes or modification, these equivalent forms also fall within the scope of the appended claims of the present application.
Claims (6)
1. producing Yellow Fever Vaccine,Live production technology with chick-embryo cell culture 17D strains the present invention relates to a kind of.Its feature exists
In：Using cell factory culture chick-embryo cell as Virus culture stroma cell, 17D of the inoculation through chick-embryo cell adaptation of virus subtracts
Toxic vaccine strain seed culture of viruses collects virus liquid by culture, after filtering out bacterium, freeze drying protectant is added, freeze-drying prepares finished product epidemic disease
Seedling.
2. preparation method according to claim 1, the 17D attenuated vaccine strain seeds culture of viruses used in the present invention are the World Health Organization
(WHO) the 17D-204 strains (NIBS CODE213-77) provided.Chick-embryo cell is prepared with SPF chicken embryos.Chicken used in the present invention
Blastocyte seed culture of viruses is seed culture of viruses of the inventor through chick-embryo cell adaptation of virus.Seed culture of viruses titre presses mouse LD50Method titre is not less than
5.60LD50；The titration of plaque method is not less than 5.8LgPFU/mL.
3. preparation method according to claim 1, this vaccine bebcell cultural method are：
1) trypsin digestion and cells concentration 0.25%, pH are 7.4~7.6.
2) chick-embryo cells culture solution is containing 5% newborn bovine serum, 0.3% lactoalbumin hydrolysate, 1%NaHCO3, 2mM glutamine
MEM culture mediums.
3) viruses culture solutions are the DMEM solution without cow's serum, and 2%~3%NaHCO is added3, pH7.4~7.6 are mended simultaneously
Add 0.5% human albumin.
4) for the culture of chick-embryo cells using cell factory as culture carrier, cultivation temperature is 37 ± 0.1.
4. vaccine preparation method according to claim 1, vaccine preparation uses method：
1) viruses point kind method：Using cell factory culture chick-embryo cell.Chick-embryo cell presses 2~8 × 10 after digesting5/cm2Carefully
Born of the same parents' number is inoculated into cell factory, and cell culture fluid is added, and 37 ± 0.1 DEG C of cultures wait for that cell is followed by kind of a chick-embryo cell poison at single layer
Kind.
It is trained the cell of single layer, first the culture solution of falling virus removal, by 0.01~0.5 virus inoculations of MOI.It is thin after virus inoculation
Born of the same parents set 35 DEG C~37 DEG C 4~6 hours, and virus is made to come into full contact with absorption with cell.Then prevent or cure a disease venom, use phosphate buffer
(PBS) cell surface is rinsed, is eventually adding the virus-culturing fluids of pH7.4~7.6, be sent into 35 DEG C~37 DEG C thermostatic chambers and carry out
Culture.
2) viral miscegenation method is：After postdigestive cell suspension is sufficiently mixed by 0.001~0.1MOI virus inoculations, packing is extremely
Cell factory sets 35 DEG C~37 DEG C cultures.
The culture solution of falling virus removal after culture 48 hours, is rinsed cell surface with phosphate buffer (PBS), is eventually adding
35 DEG C~37 DEG C cultures are sent into virus-culturing fluid, pH7.4~7.6.
5. vaccine preparation method according to claim 1, the two methods virus harvest time be from after being inoculated with culture 72~
Start harvest virus after 96 hours.The virus liquid of harvest is again through 0.22 μm of aseptic filtration after 1 μm or the clarification of 0.6 μm of filter membrane
Stoste, freeze drying protectant is added after assay approval carries out freeze-drying vaccine is made.
6. preparation method according to claim 1, the ingredient that the vaccine protects freeze-drying shield agent solution are：Without gelatin
PH7.4~7.6DMEM polysaccharide solutions.
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| CN111909905B (en) * | 2020-09-14 | 2022-04-12 | 天津中逸安健生物科技有限公司 | Concentration and preservation method of mumps attenuated live vaccine virus |
| CN112342185A (en) * | 2020-11-11 | 2021-02-09 | 深圳市卫光生物制品股份有限公司 | Bioreactor process for chick embryo cell rabies virus |
| CN112342185B (en) * | 2020-11-11 | 2022-12-02 | 深圳市卫光生物制品股份有限公司 | Bioreactor process for chick embryo cell rabies virus |
| CN113373107A (en) * | 2021-01-29 | 2021-09-10 | 上海生物制品研究所有限责任公司 | Method for producing virus by preparing passage chick embryo cells in large scale |
| CN113373107B (en) * | 2021-01-29 | 2022-12-30 | 上海生物制品研究所有限责任公司 | Method for producing virus by preparing passage chick embryo cells in large scale |
| CN115627259A (en) * | 2022-11-30 | 2023-01-20 | 北京赛尔富森生物科技有限公司 | Adaptation method of virus in chick embryo fibroblast |
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