CN108433013A - A kind of preparation method of natural Beta-carotene preparation - Google Patents
A kind of preparation method of natural Beta-carotene preparation Download PDFInfo
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- CN108433013A CN108433013A CN201810183408.5A CN201810183408A CN108433013A CN 108433013 A CN108433013 A CN 108433013A CN 201810183408 A CN201810183408 A CN 201810183408A CN 108433013 A CN108433013 A CN 108433013A
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- carotene
- natural beta
- emulsification pretreatment
- water
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- 238000002360 preparation method Methods 0.000 title claims abstract description 110
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 title claims abstract description 75
- 235000013734 beta-carotene Nutrition 0.000 title claims abstract description 75
- 239000011648 beta-carotene Substances 0.000 title claims abstract description 75
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 title claims abstract description 75
- 229960002747 betacarotene Drugs 0.000 title claims abstract description 75
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 title claims abstract description 75
- 238000004945 emulsification Methods 0.000 claims abstract description 64
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 43
- 239000000463 material Substances 0.000 claims abstract description 24
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 21
- 150000001875 compounds Chemical class 0.000 claims abstract description 20
- 239000008367 deionised water Substances 0.000 claims abstract description 16
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 16
- 239000000839 emulsion Substances 0.000 claims abstract description 13
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000000265 homogenisation Methods 0.000 claims abstract description 6
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 12
- 235000010413 sodium alginate Nutrition 0.000 claims description 12
- 239000000661 sodium alginate Substances 0.000 claims description 12
- 229940005550 sodium alginate Drugs 0.000 claims description 12
- 229920002752 Konjac Polymers 0.000 claims description 11
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims description 10
- 235000010445 lecithin Nutrition 0.000 claims description 10
- 239000000787 lecithin Substances 0.000 claims description 10
- 229940067606 lecithin Drugs 0.000 claims description 10
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 claims description 10
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims description 9
- 229920002581 Glucomannan Polymers 0.000 claims description 9
- 229940046240 glucomannan Drugs 0.000 claims description 9
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims description 8
- 238000010008 shearing Methods 0.000 claims description 5
- 210000000481 breast Anatomy 0.000 claims description 4
- 230000008859 change Effects 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 17
- 239000000049 pigment Substances 0.000 abstract description 8
- 230000000052 comparative effect Effects 0.000 description 22
- 235000021466 carotenoid Nutrition 0.000 description 9
- 150000001747 carotenoids Chemical class 0.000 description 9
- 230000014759 maintenance of location Effects 0.000 description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 244000000626 Daucus carota Species 0.000 description 5
- 235000002767 Daucus carota Nutrition 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000003960 organic solvent Substances 0.000 description 5
- 239000000843 powder Substances 0.000 description 5
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000002203 pretreatment Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- 244000088415 Raphanus sativus Species 0.000 description 3
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- ANVAOWXLWRTKGA-XHGAXZNDSA-N all-trans-alpha-carotene Chemical compound CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1C(C)=CCCC1(C)C ANVAOWXLWRTKGA-XHGAXZNDSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 239000012065 filter cake Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 241000432824 Asparagus densiflorus Species 0.000 description 1
- HRQKOYFGHJYEFS-UHFFFAOYSA-N Beta psi-carotene Chemical compound CC(C)=CCCC(C)=CC=CC(C)=CC=CC(C)=CC=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C HRQKOYFGHJYEFS-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 235000003903 alpha-carotene Nutrition 0.000 description 1
- 239000011795 alpha-carotene Substances 0.000 description 1
- ANVAOWXLWRTKGA-HLLMEWEMSA-N alpha-carotene Natural products C(=C\C=C\C=C(/C=C/C=C(\C=C\C=1C(C)(C)CCCC=1C)/C)\C)(\C=C\C=C(/C=C/[C@H]1C(C)=CCCC1(C)C)\C)/C ANVAOWXLWRTKGA-HLLMEWEMSA-N 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000002019 anti-mutation Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000000633 gamma-carotene Nutrition 0.000 description 1
- 239000011663 gamma-carotene Substances 0.000 description 1
- HRQKOYFGHJYEFS-RZWPOVEWSA-N gamma-carotene Natural products C(=C\C=C\C(=C/C=C/C=C(\C=C\C=C(/C=C/C=1C(C)(C)CCCC=1C)\C)/C)\C)(\C=C\C=C(/CC/C=C(\C)/C)\C)/C HRQKOYFGHJYEFS-RZWPOVEWSA-N 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 150000008282 halocarbons Chemical class 0.000 description 1
- 230000000215 hyperchromic effect Effects 0.000 description 1
- 235000010485 konjac Nutrition 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000013557 residual solvent Substances 0.000 description 1
- 238000005245 sintering Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001694 spray drying Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/40—Colouring or decolouring of foods
- A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
- A23L5/43—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives
- A23L5/44—Addition of dyes or pigments, e.g. in combination with optical brighteners using naturally occurring organic dyes or pigments, their artificial duplicates or their derivatives using carotenoids or xanthophylls
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/03—Organic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Polymers & Plastics (AREA)
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- Mycology (AREA)
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- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
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Abstract
The invention belongs to natural pigment technical fields, and in particular to a kind of preparation method of natural Beta-carotene preparation.The preparation method of natural Beta-carotene preparation provided by the invention includes the following steps：Natural Beta-carotene is taken, compound emulsifying agent is added, is uniformly mixed, obtains oil phase；Ionized water is removed, water-soluble high-molecular material is added in above-mentioned deionized water, stirs to being completely dissolved, obtains water phase；Gained oil phase is added in gained water phase, emulsification pretreatment twice, obtains emulsion；Homogeneous is carried out to gained emulsion, homogenization pressure is 50 100Mpa, and homogenizing time is 2 4min.The preparation method of natural Beta-carotene preparation provided by the invention is simple, the good, good stability using natural Beta-carotene preparation water dispersible made from method provided by the invention, and easy to use.In addition, natural Beta-carotene preparation grain size made from method using the present invention is smaller, its bioavilability can be significantly improved.
Description
Technical field
The invention belongs to natural pigment technical fields, and in particular to a kind of preparation method of natural Beta-carotene preparation.
Background technology
Natural Beta-carotene primary raw material source is palm fruit, the one kind obtained through techniques such as molecularly distilled extraction, concentrations
Natural mixed carotenoid, color are orange-yellow, the experiment proved that the healthcare function with anti-aging and anti-mutation, by a variety of
The mixture of carrotene composition, mainly has alpha-carotene, beta carotene and a small amount of gamma carotene, bronzing paste sticky
Liquid has special odor, is dissolved in grease but not soluble in water, is slightly soluble in ethyl alcohol, heat-resisting, acid resistance is good, light resistance elementary errors.
Chinese patent CN100421650C discloses a kind of preparation method of water-dispersed carotenoid powder, and steps are as follows：
Carotenoid coarse crystallization is dissolved in containing in antioxidant, the halogenated hydrocarbons of emulsifier or esters class solvent, is added to Sprayable
In the ethyl alcohol or isopropanol of stirring, carotenoid is made to be precipitated in the form of amorphous powder；It is precipitated with filter membrane or sintering filter stick filtering
Carotenoid, filter cake washs with ethyl alcohol or isopropanol, is filtered dry；Filter cake is added in the aqueous solution containing protecting colloid, is first stirred
Mashing is mixed, by emulsifying, residual solvent is then removed, aqueous dispersions is made；Dispersion liquid is granulated with spray-on process, then through stream
Stateization is dried.Although the invention easily removes solvent, less residue, removing is fast, efficient, required carotenoid easy to control
Grain size and color and luster, but troublesome precipitation process, low production efficiency must be added in the invention before emulsification, and uses
Inevitably there is dissolvent residual in a large amount of organic solvent, in addition products obtained therefrom is powder, is not directly used.
US3998753 discloses a kind of dispersible carotenoid preparation method of water, and the granularity of wherein carotenoid is small
In 1 μm, this method is that carotenoid and other additives are first made into the solution of organic solvent, is then added to containing gelatin, divides
Powder, stabilizer aqueous solution in, make System forming lotion through high speed shear；Remove it is organic molten, spray drying obtain required powder
End.The invention a large amount of solvents, required time contained in removing system need to be grown in protecting colloid, and it is clean to be not easy removing.
In conclusion still lacking in the prior art, a kind of preparation method is simple, natural Beta-carotene preparation moisture obtained
Scattered property is good, grain size is smaller, without organic solvent is used, to avoid the problem that organic solvent residual, bioavilability height, stability
It is good, and the preparation method for the natural Beta-carotene preparation that can be used directly.
Invention content
In view of the deficiencies of the prior art, the purpose of the present invention is to provide a kind of preparation methods of natural Beta-carotene preparation.
The preparation method of natural Beta-carotene preparation provided by the invention is simple, using natural carrot made from method provided by the invention
Plain preparation water dispersible is good, good stability, and using when can be directly added into liquid component, it is easy to use.In addition, using this hair
Natural Beta-carotene preparation grain size made from bright method is smaller, can significantly improve its bioavilability, and organic solvent-free is residual
It stays.
The technical scheme is that：
A kind of preparation method of natural Beta-carotene preparation, includes the following steps：
S1 takes natural Beta-carotene, compound emulsifying agent is added, the addition of compound emulsifying agent is above-mentioned natural carrot quality
The 30%-40% of amount is uniformly mixed, obtains oil phase；
S2 removes ionized water, and the quality of deionized water is 3-5 times of natural Beta-carotene quality in step S1, will be water-soluble
High molecular material is added in above-mentioned deionized water, and the addition of water-soluble high-molecular material is the 4- of above-mentioned deionized water quality
6%, it stirs to being completely dissolved, obtains water phase；
Oil phase obtained by step S1 is added in water phase obtained by step S2 by S3, and emulsification pretreatment twice, obtains emulsion；
S4 carries out homogeneous to emulsion obtained by step S3, and homogenization pressure 50-100Mpa, homogenizing time 2-4min are obtained
Natural Beta-carotene preparation.
Further, compound emulsifying agent presses quality by lecithin, median chain triglyceride oil and Tween 80 in the step S1
Compare 6-9:4-6:1-3 is formed.
Further, compound emulsifying agent presses matter by lecithin, median chain triglyceride oil and Tween 80 in the step S1
Measure ratio 8:5:2 compositions.
Further, in the step S2 water-soluble high-molecular material by chitosan oligosaccharide, sodium alginate and konjaku glucomannan
15-18 in mass ratio:7-10:1-3 is formed.
Further, in the step S2 water-soluble high-molecular material by chitosan oligosaccharide, sodium alginate and konjak portuguese gansu polyose
Sugar in mass ratio 16:9:2 compositions.
Preferably, the average molecular weight of the chitosan oligosaccharide is 1000-3000.
It is further preferred that the average molecular weight of the chitosan oligosaccharide is 2000.
Further, twice, the temperature of first time emulsification pretreatment is 70-80 DEG C to the step S3 emulsification pretreatments, shearing breast
The speed of change is 13000-16000r/min, and the time of emulsification pretreatment is 1-3min；The temperature of second of emulsification pretreatment is 70-80
DEG C, the speed of emulsification pretreatment is 4000-6000r/min, and the time of emulsification pretreatment is 5-8min.
Further, twice, the temperature of first time emulsification pretreatment is 70 DEG C to the step S3 emulsification pretreatments, emulsification pretreatment
Speed be 15000r/min, time of emulsification pretreatment is 2min；The temperature of second of emulsification pretreatment is 70 DEG C, emulsification pretreatment
Speed is 5000r/min, and the time of emulsification pretreatment is 6min.
Be added in the present invention by chitosan oligosaccharide, sodium alginate and konjaku glucomannan by certain mass than the water solubility that forms
High molecular material can not only wrap up drop, play a protective role, and further increase the stability of product, and to natural Hu trailing plants
Bu Su has protection and hyperchromic effect, can improve the pigment retention rate of product.
In the present invention, emulsification pretreatment twice has been carried out, first time emulsification pretreatment speed (13000-16000r/min),
Dispersion and emulsion efficiency early period of drop can be improved, second of emulsification pretreatment speed relatively slow (4000-6000r/min) can make drop
It is spaced further apart and disperses, improve emulsifying power, so that the drop after emulsification is reached suitable grain size, and keep size droplet diameter size equal
It is even, so as to improve the stability and using effect of gained natural Beta-carotene preparation.
Compared with prior art, the present invention has the advantage that：
(1) preparation method of natural Beta-carotene preparation provided by the invention is simple, is easy to industrialized production, and produce effect
Rate is high.
(2) use natural Beta-carotene preparation made from the method for the present invention that there is good dispersibility, and stability in water
It is good.
(3) use natural Beta-carotene preparation made from the method for the present invention for mobility liquid, when use can be added directly into
It needs in the liquid component being added to, it is easy to use.
(4) preparation method of natural Beta-carotene preparation provided by the invention is simple, and without adding organic solvent hydrotropy,
It ensure that the safety of products obtained therefrom.
Specific implementation mode
The following describes the present invention further through the description of specific embodiments, but this is not the limit to the present invention
System, those skilled in the art's basic thought according to the present invention can make various modifications or improvements, but without departing from this
The basic thought of invention, is all within the scope of the present invention.
In the present invention, lecithin is purchased from Jiangsu Tian De food additives Co., Ltd, food-grade；Median chain triglyceride oil
It is purchased from Guangzhou Tao Sheng Chemical Co., Ltd.s, model：005, food-grade；Tween 80 is purchased from the limited public affairs of Guangzhou closest friend's additive
Department, food-grade；Sodium alginate is purchased from Shaanxi Sen Fu natural products Co., Ltd, model：SF021HZSN, food-grade；Konjaku Portugal
Sweet glycan is purchased from Xi'an asparagus fern bio tech ltd, food-grade.
Embodiment 1, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation, includes the following steps：
S1 takes natural Beta-carotene, compound emulsifying agent is added, the addition of compound emulsifying agent is above-mentioned natural carrot quality
The 30% of amount is uniformly mixed, obtains oil phase；
S2 removes ionized water, and the quality of deionized water is 3 times of natural Beta-carotene quality in step S1, by high water solubility
Molecular material is added in above-mentioned deionized water, and the addition of water-soluble high-molecular material is the 4% of above-mentioned deionized water quality,
Stirring obtains water phase to being completely dissolved；
Oil phase obtained by step S1 is added in water phase obtained by step S2 by S3, emulsification pretreatment twice, first time emulsification pretreatment
Temperature be 70 DEG C, the speed of emulsification pretreatment is 13000r/min, and the time of emulsification pretreatment is 1min；Second emulsification pretreatment
Temperature is 70 DEG C, and the speed of emulsification pretreatment is 4000r/min, and the time of emulsification pretreatment is 5min, obtains emulsion；
S4 carries out homogeneous to emulsion obtained by step S3, and homogenization pressure 50Mpa, homogenizing time 2min are obtained naturally recklessly
Radish element preparation.
Compound emulsifying agent is by lecithin, median chain triglyceride oil and Tween 80 in mass ratio 6 in the step S1:6:3 groups
At.
Chitosan oligosaccharide, sodium alginate and the konjaku that water-soluble high-molecular material is 1000 by average molecular weight in the step S2
Glucomannan in mass ratio 15:10:3 compositions.
Embodiment 2, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation, includes the following steps：
S1 takes natural Beta-carotene, compound emulsifying agent is added, the addition of compound emulsifying agent is above-mentioned natural carrot quality
The 40% of amount is uniformly mixed, obtains oil phase；
S2 removes ionized water, and the quality of deionized water is 5 times of natural Beta-carotene quality in step S1, by high water solubility
Molecular material is added in above-mentioned deionized water, and the addition of water-soluble high-molecular material is the 6% of above-mentioned deionized water quality,
Stirring obtains water phase to being completely dissolved；
Oil phase obtained by step S1 is added in water phase obtained by step S2 by S3, emulsification pretreatment twice, first time emulsification pretreatment
Temperature be 80 DEG C, the speed of emulsification pretreatment is 16000r/min, and the time of emulsification pretreatment is 3min；Second emulsification pretreatment
Temperature is 80 DEG C, and the speed of emulsification pretreatment is 6000r/min, and the time of emulsification pretreatment is 8min, obtains emulsion；
S4 carries out homogeneous to emulsion obtained by step S3, and homogenization pressure 100Mpa, homogenizing time 4min are obtained naturally recklessly
Radish element preparation.
Compound emulsifying agent is by lecithin, median chain triglyceride oil and Tween 80 in mass ratio 9 in the step S1:4:1 group
At.
Chitosan oligosaccharide, sodium alginate and the konjaku that water-soluble high-molecular material is 3000 by average molecular weight in the step S2
Glucomannan in mass ratio 18:7:1 composition.
Embodiment 3, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation, includes the following steps：
S1 takes natural Beta-carotene, compound emulsifying agent is added, the addition of compound emulsifying agent is above-mentioned natural carrot quality
The 35% of amount is uniformly mixed, obtains oil phase；
S2 removes ionized water, and the quality of deionized water is 4 times of natural Beta-carotene quality in step S1, by high water solubility
Molecular material is added in above-mentioned deionized water, and the addition of water-soluble high-molecular material is the 5% of above-mentioned deionized water quality,
Stirring obtains water phase to being completely dissolved；
Oil phase obtained by step S1 is added in water phase obtained by step S2 by S3, emulsification pretreatment twice, first time emulsification pretreatment
Temperature be 70 DEG C, the speed of emulsification pretreatment is 15000r/min, and the time of emulsification pretreatment is 2min；Second emulsification pretreatment
Temperature is 70 DEG C, and the speed of emulsification pretreatment is 5000r/min, and the time of emulsification pretreatment is 6min, obtains emulsion；
S4 carries out homogeneous to emulsion obtained by step S3, and homogenization pressure 70Mpa, homogenizing time 3min are obtained naturally recklessly
Radish element preparation.
Compound emulsifying agent is by lecithin, median chain triglyceride oil and Tween 80 in mass ratio 8 in the step S1:5:2 groups
At.
Chitosan oligosaccharide, sodium alginate and the konjaku that water-soluble high-molecular material is 2000 by average molecular weight in the step S2
Glucomannan in mass ratio 16:9:2 compositions.
Comparative example 1, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, compound emulsifying agent is pressed by lecithin and median chain triglyceride oil in the step S1
Mass ratio 8:5 compositions.
Comparative example 2, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, compound emulsifying agent by lecithin, median chain triglyceride oil and is spat in the step S1
Temperature 80 in mass ratio 1:1:1 composition.
Comparative example 3, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, water-soluble high-molecular material is 2000 by average molecular weight in the step S2
Chitosan oligosaccharide and sodium alginate in mass ratio 16:9 compositions.
Comparative example 4, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, water-soluble high-molecular material is 2000 by average molecular weight in the step S2
Chitosan oligosaccharide, sodium alginate and konjaku glucomannan in mass ratio 1:1:1 composition.
Comparative example 5, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, water-soluble high-molecular material is 2500 by average molecular weight in the step S2
Chitosan oligosaccharide, sodium alginate and konjaku glucomannan in mass ratio 16:9:2 compositions.
Comparative example 6, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, twice, the temperature of first time emulsification pretreatment is 70 to the step S3 emulsification pretreatments
DEG C, the speed of emulsification pretreatment is 5000r/min, and the time of emulsification pretreatment is 6min；The temperature of second of emulsification pretreatment is 70 DEG C,
The speed of emulsification pretreatment is 15000r/min, and the time of emulsification pretreatment is 2min.
Comparative example 7, a kind of preparation method of natural Beta-carotene preparation
The preparation method of the natural Beta-carotene preparation is similar to Example 3.
It is with the difference of embodiment 3, the step S3 emulsification pretreatments are primary, and the temperature of emulsification pretreatment is 70 DEG C, shearing
The speed of emulsification is 15000r/min, and the time of emulsification pretreatment is 2min.
The pigment retention rate test of test example one, natural Beta-carotene preparation
1, test material：Using embodiment 1, the method for embodiment 2, embodiment 3, comparative example 3, comparative example 4, comparative example 5
Natural Beta-carotene preparation obtained.
2, test method：
The percentage composition and color value that natural Beta-carotene preparation is detected using spectrophotometer, solvent, wavelength are done with acetone
Absorbance value is detected at 450nm absorption maximum wave crests, calculates E0, after three months, six months, nine months, 12 months respectively
E1, E2, E3, E4 are obtained by same procedure detection, obtain pigment retention rate.3, test result is as shown in table 1.
Table 1：The pigment retention rate of natural Beta-carotene preparation compares
As can be seen from Table 1, the pigment retention rate of natural Beta-carotene preparation made from method using the present invention is higher,
Wherein, the pigment retention rate highest of embodiment 3 is highly preferred embodiment of the present invention；Compared with comparative example 3-5, the color of embodiment 3
Plain retention rate higher.This illustrates that natural Beta-carotene preparation made from method using the present invention is with good stability.
Test example two, the test of the particle size of natural Beta-carotene preparation
1, test material：Using day made from embodiment 3, comparative example 1, comparative example 2, comparative example 6, the method for comparative example 7
Right carotene preparation.
2, test method：
With LA-920 laser particle size analyzers to using embodiment 3, comparative example 1, comparative example 2, comparative example 6, comparative example 7
Natural Beta-carotene preparation made from method carries out particle size test.
3, test result is as shown in table 2.
Table 2：The particle size test result of natural Beta-carotene preparation compares
As can be seen from Table 2, the grain size of natural Beta-carotene preparation made from method using the present invention is smaller；With comparison
Example 1, comparative example 2, comparative example 6, comparative example 7 are compared, and the grain size smaller of embodiment 3 significantly improves natural Beta-carotene preparation
Bioavilability.
Claims (9)
1. a kind of preparation method of natural Beta-carotene preparation, which is characterized in that include the following steps：
S1 takes natural Beta-carotene, compound emulsifying agent is added, the addition of compound emulsifying agent is above-mentioned natural Beta-carotene quality
30%-40% is uniformly mixed, obtains oil phase；
S2 removes ionized water, and the quality of deionized water is 3-5 times of natural Beta-carotene quality in step S1, by water-soluble high score
Sub- material is added in above-mentioned deionized water, and the addition of water-soluble high-molecular material is the 4-6% of above-mentioned deionized water quality,
Stirring obtains water phase to being completely dissolved；
Oil phase obtained by step S1 is added in water phase obtained by step S2 by S3, and emulsification pretreatment twice, obtains emulsion；S4 is to step S3
Gained emulsion carries out homogeneous, and homogenization pressure 50-100Mpa, homogenizing time 2-4min obtain natural Beta-carotene preparation.
2. the preparation method of natural Beta-carotene preparation as described in claim 1, which is characterized in that compound in the step S1
Emulsifier is by lecithin, median chain triglyceride oil and Tween 80 6-9 in mass ratio:4-6:1-3 is formed.
3. the preparation method of natural Beta-carotene preparation as claimed in claim 2, which is characterized in that compound in the step S1
Emulsifier is by lecithin, median chain triglyceride oil and Tween 80 in mass ratio 8:5:2 compositions.
4. the preparation method of natural Beta-carotene preparation as described in claim 1, which is characterized in that water-soluble in the step S2
Property high molecular material is by chitosan oligosaccharide, sodium alginate and konjaku glucomannan 15-18 in mass ratio:7-10:1-3 is formed.
5. the preparation method of natural Beta-carotene preparation as claimed in claim 4, which is characterized in that water-soluble in the step S2
Property high molecular material is by chitosan oligosaccharide, sodium alginate and konjaku glucomannan in mass ratio 16:9:2 compositions.
6. the preparation method of natural Beta-carotene preparation as claimed in claim 4, which is characterized in that the chitosan oligosaccharide is averaged
Molecular weight is 1000-3000.
7. the preparation method of natural Beta-carotene preparation as claimed in claim 6, which is characterized in that the chitosan oligosaccharide is averaged
Molecular weight is 2000.
8. the preparation method of natural Beta-carotene preparation as described in claim 1, which is characterized in that the step S3 shearings breast
Change twice, the temperature of first time emulsification pretreatment is 70-80 DEG C, and the speed of emulsification pretreatment is 13000-16000r/min, shearing breast
The time of change is 1-3min；The temperature of second of emulsification pretreatment is 70-80 DEG C, and the speed of emulsification pretreatment is 4000-6000r/
The time of min, emulsification pretreatment are 5-8min.
9. the preparation method of natural Beta-carotene preparation as claimed in claim 8, which is characterized in that the step S3 shearings breast
Change twice, the temperature of first time emulsification pretreatment is 70 DEG C, and the speed of emulsification pretreatment is 15000r/min, and the time of emulsification pretreatment is
2min；The temperature of second of emulsification pretreatment is 70 DEG C, and the speed of emulsification pretreatment is 5000r/min, and the time of emulsification pretreatment is
6min。
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| CN113243476A (en) * | 2021-05-07 | 2021-08-13 | 广州市纳趣尔食品有限责任公司 | Compound natural carotene pigment product and preparation method thereof |
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