CN108432994A - A kind of algae drink preparation method and gained algae drink based on marine microorganism ferment - Google Patents

A kind of algae drink preparation method and gained algae drink based on marine microorganism ferment Download PDF

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Publication number
CN108432994A
CN108432994A CN201810174664.8A CN201810174664A CN108432994A CN 108432994 A CN108432994 A CN 108432994A CN 201810174664 A CN201810174664 A CN 201810174664A CN 108432994 A CN108432994 A CN 108432994A
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China
Prior art keywords
algae
ferment
preparation
drink
hops
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Pending
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CN201810174664.8A
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Chinese (zh)
Inventor
尹花
董建军
余俊红
田玉红
钱中华
常宗明
闫鹏
张宇昕
陈华磊
刘晓琳
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Tsingtao Brewery Co Ltd
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Tsingtao Brewery Co Ltd
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Priority to CN201810174664.8A priority Critical patent/CN108432994A/en
Publication of CN108432994A publication Critical patent/CN108432994A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof

Abstract

The present invention proposes a kind of algae drink preparation method based on marine microorganism ferment and gained algae drink, belong to food processing technology field, the algae drink that can be solved the problems, such as seaweed fishy smell, while make has good potableness and retains the corresponding function of algae.The technical solution includes the preparation of algae ferment, the preparation of hops tea, the preparation for pre-processing algae drink and is carbonated to obtain several steps such as algae drink to it.Algae drink provided by the invention solves the problems, such as seaweed fishy smell, while having good potableness and retaining the corresponding function of algae.

Description

A kind of algae drink preparation method and gained algae based on marine microorganism ferment Drink
Technical field
The invention belongs to food processing technology field more particularly to a kind of algae drink systems based on marine microorganism ferment Preparation Method and gained algae drink.
Background technology
Seaweed is important living marine resources, in recent years, as people go deep into the gradual of living marine resources research, Bioactive substance and its biological function in seaweed are increasingly valued by people, and become the hot spot studied both at home and abroad.
China's marine algae resource is extremely abundant, is global maximum seaweed industry state, is provided for seaweed processing industry huge Raw material basis and advantage.Rich in polysaccharide, Polyphenols, terpene, protein, amino acid, unsaturated fatty acid isoreactivity in seaweed Substance provides various biological function, such as antioxidant activity, adjusting immunocompetence for seaweed.Pay close attention to the activity in seaweed Ingredient and biological function research and develop novel functional sea food and have great importance.But since seaweed itself has Fishy smell keeps its potableness when directly drinking poor, cannot be satisfied expectation of the consumer to beverage.
Invention content
The present invention proposes a kind of algae drink preparation method based on marine microorganism ferment and gained algae drink, the drink Product solve the problems, such as seaweed fishy smell, while the algae drink made has good potableness and retains the corresponding work(of algae Energy.
In order to achieve the above object, an aspect of of the present present invention provides a kind of red algae drink based on marine microorganism ferment Preparation method includes the following steps:
The preparation of algae ferment:Fermentation tank will be moved into through shearing after fresh algae raw material or dry algae rehydration plus after water homogenisation Middle sterilizing, the fermentation substrate after sterilizing is successively accessed under different temperatures bacillus subtilis suspension and lactobacillus plantarum suspension into Seaweed ferment after fermentation is obtained algae ferment by row fermentation by being separated by solid-liquid separation;
The preparation of hops tea:Pure water by hop pellets and after boiling is according to 1:The ratio of 100-150 is prepared, after preparation Continue to boil 1min, stops heating and being cooled to room temperature, hops tea is obtained by filtration;
Pre-process the preparation of algae drink:By mass fraction by algae ferment 1.5%-15%, hops tea 1%-10%, sugar 2%-5%, acid flavoring 0.01%-0.05% are starched, surplus is to be stirred evenly in water input material-compound tank, obtains pretreatment algae drink Product;
Carbonating:Pretreatment algae drink is carbonated with carbon dioxide, obtains algae drink.
Preferably, the algae includes red algae and brown alga, the algae ferment is the mixed of red algae ferment and brown alga ferment Close object, wherein the mass fraction of the red algae ferment of addition is 0.5-5%, the mass fraction of brown alga ferment is 1-10%.
Preferably, the mass fraction of the red algae ferment of addition is 0.5%-2%, the mass fraction of brown alga ferment is 2- 5%, the coefficient optimum proportion of hops tea is 2-6%.
Preferably, carbon dioxide content is 0.35%-0.65% in the algae drink.
Preferably, the fermentation substrate after sterilizing is hanged by the sterile prepared bacillus subtilis of access of inoculum concentration 1.5% Liquid ferments 3 days in 40 DEG C of stirrings, stops stirring, then cool the temperature to 30 DEG C, prepared by the sterile access of inoculum concentration 1% Lactobacillus plantarum suspension stirs 5 minutes in 30 DEG C, stops stirring, stationary culture 2 days.
Preferably, the syrup is at least one of fructose syrup or glucose syrup.
Preferably, the acid flavoring is at least one of citric acid, malic acid or lactic acid.
Preferably, the hops are aroma hops or bitter fragrant inclusive type hops, selected from Qingdao great Hua, Tettanger, Any one in Saaz, Hallertauer MFR, Cascade or Citral.
Preferably, the red algae includes at least one of seaweed, agar, asparagus, Irish moss or Eucheuma, institute It includes at least one of kelp, kelp, undaria pinnitafida or sargassum fusifome to state brown alga.
Another aspect provides a kind of preparation methods as described in any of the above-described technical solution to be prepared Algae drink.
Compared with prior art, the advantages and positive effects of the present invention are:
Algae drink provided by the invention remains the functions such as anti-oxidant and brown alga the anticoagulation of red algae, brown alga, mouth Allocated using pure natural hops tea on taste, solve the problems, such as the fishy smell of seaweed, at the same make algae drink mouthfeel it is clean, It is tasty and refreshing, have the slight fragrance of a flower, potableness preferable.
Specific implementation mode
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common The every other embodiment that technical staff is obtained without making creative work belongs to the model that the present invention protects It encloses.
The red algae drink preparation method based on marine microorganism ferment that an embodiment of the present invention provides a kind of, including following step Suddenly:
S1:The preparation of algae ferment:
Selection is clean and meets red algae and the brown alga raw material of food hygienic standard, respectively by the fresh raw material of red algae and brown alga or Through shearing after dry raw material rehydration, by weight 1:10 ratio moves into fermentation tank after adding water homogenisation, and stirring sterilizing is cooled to 40 ± 2 It is DEG C spare.By food grade bacillus subtilis solid dry bacteria (1010CFU/g it is configured to sterile saline before) being inoculated with 10% bacteria suspension, plant lactobacillus powder microbial inoculum (109CFU/g) be configured to sterile saline before inoculation 10% bacterium it is outstanding Liquid.By under the 40 DEG C of keeping warm modes of fermentation substrate to have sterilized, hanged by the sterile prepared bacillus subtilis of access of inoculum concentration 1.5% Liquid, stirring fermentation, fermentation condition:40 DEG C ferment 3 days, stop stirring, terminate fermentation.Then system temperature is down to 30 DEG C, pressed The sterile prepared lactobacillus plantarum suspension of access of inoculum concentration 1%, 30 DEG C are stirred 5 minutes, stop stirring, stationary culture 2 days, pH4-5.The mixture fermented is obtained into red algae ferment and brown alga ferment by separation of solid and liquid.In this step, the algae packet Include red algae and brown alga, obtained red algae ferment and brown alga ferment have proteinase activity, an amylase activity, while red algae, brown Contain a large amount of seaweed activated oligosaccharides of fermented generation in algae ferment.
S2:The preparation of hops tea:
Pure water by hop pellets and after boiling is according to 1:The ratio of 100-150 is prepared, and continues to boil 1min after preparation, Stop heating and being cooled to room temperature, hops tea is obtained by filtration.
In the step, the amount for the pure water being added can obtain meeting the hops tea subsequently needed within the above range. It is understood that within the above range, the proportioning of hop pellets and the pure water after boiling can also be 1:110、1:120、 1:130、1:140 or match other arbitrary point values within the scope of this.
S3:Pre-process the preparation of algae drink:By mass fraction by algae ferment 1.5%-15%, hops tea 1%- 10%, syrup 2%-5%, acid flavoring 0.01%-0.05%, surplus are to be stirred evenly in water input material-compound tank, are pre-processed Algae drink.Wherein, the mass fraction of the red algae ferment of addition is 0.5-5%, the mass fraction of brown alga ferment is 1-10%.
In the step, by by above-mentioned component compatibility, being allocated, can be effectively solved from taste with natural hops tea The fishy smell problem of seaweed so that obtained pretreatment algae drink mouthfeel is clean, it is tasty and refreshing, have a slight fragrance of a flower, potableness compared with It is good.Wherein, the amount that red algae ferment is added can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% etc., brown alga ferment The amount that element is added can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% etc., the addition of hops tea can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9% etc., the addition of syrup can be 2.5%, 3%, 3.5%, 4%, 4.5% etc., acid flavoring Addition can be 0.02%, 0.03%, 0.04% etc..
S4:Carbonating:Pretreatment algae drink is carbonated with carbon dioxide, obtains algae drink.
In the step, carbonating is carried out to pretreatment algae drink using carbon dioxide, final algae drink is prepared. In a preferred embodiment, carbon dioxide content is 0.35%-0.65% in the algae drink.Under the carbon dioxide content Drink mouthfeel is tasty and refreshing, and potableness is preferable.
In a preferred embodiment, the mass fraction of the red algae ferment of addition is 0.5%-2%, the mass parts of brown alga ferment Number is 0.5-1%, and hops tea is 2-6%.In the present embodiment, to red algae ferment, brown alga ferment and the hops tea in formula Addition is advanced optimized, and is effectively removed the fishy smell problem of algae ferment in drink with realization and is adjusted obtained The sensory issues of algae drink.
In a preferred embodiment, the syrup is at least one of fructose syrup or glucose syrup.It is preferred real one It applies in example, the acid flavoring is at least one of citric acid, malic acid or lactic acid.In a preferred embodiment, the hops For aroma hops or bitter fragrant inclusive type hops, it is selected from Qingdao great Hua, Di Tenan (Tettanger), Sa scholar (Saaz), Harrar road Any one in (Hallertauer MFR), Ka Sikate (Cascade) or western Chu (Citral).In a preferred embodiment In, the red algae includes at least one of seaweed, agar, asparagus, Irish moss or Eucheuma, and the brown alga includes sea At least one of band, kelp, undaria pinnitafida or sargassum fusifome.
The specific example of syrup, acid flavoring and hops is enumerated in the various embodiments described above, it is to be understood that for each Enumerating for component is not limited to cited by above-described embodiment, and can also be familiar to those skilled in the art can rationally replace The respective components used.In addition, selected red algae, brown alga type can also be other types known in the art, and Cited by being not limited to the above embodiment.
The embodiment of the present invention additionally provides a kind of algae that the preparation method as described in any of the above-described embodiment is prepared Class drink.The algae drink remains the functions such as anti-oxidant and brown alga the anticoagulation of red algae, brown alga, while being used on taste Pure natural hops tea is allocated, and solves the problems, such as the fishy smell of seaweed so that seaweed beverage mouthfeel is clean, it is tasty and refreshing, have it is slight The fragrance of a flower, potableness it is preferable.
Below in conjunction with specific embodiment to the red algae drink preparation side provided by the invention based on marine microorganism ferment Method and gained red algae drink illustrate.
Embodiment 1
Red algae ferment 0.5%, brown alga ferment 1%, hops tea 1%, syrup 2%, acid flavoring 0.0125% are taken, according to above-mentioned Mass fraction puts into material-compound tank, and surplus is water, is stirred evenly, and pretreatment algae drink is made.Pretreatment algae obtained is drunk Product are carbonated with carbon dioxide so that carbon dioxide content is 0.4% to get to algae drink in drink.
Embodiment 2
Red algae ferment 5%, brown alga ferment 10%, hops tea 10%, syrup 5%, acid flavoring 0.05% are taken, according to above-mentioned matter It measures score and puts into material-compound tank, surplus is water, is stirred evenly, and pretreatment algae drink is made.By pretreatment algae drink obtained It is carbonated with carbon dioxide so that carbon dioxide content is 0.5% to get to algae drink in drink.
Embodiment 3
Red algae ferment 2.75%, brown alga ferment 5.5%, hops tea 5.5%, syrup 3.5%, acid flavoring 0.03% are taken, is pressed Material-compound tank is put into according to above-mentioned mass fraction, surplus is water, is stirred evenly, and pretreatment algae drink is made.By pretreatment obtained Algae drink is carbonated with carbon dioxide so that carbon dioxide content is 0.45% to get to algae drink in drink.
Embodiment 4
Red algae ferment 2%, brown alga ferment 5%, hops tea 5%, syrup 2.5%, acid flavoring 0.02% are taken, according to above-mentioned matter It measures score and puts into material-compound tank, surplus is water, is stirred evenly, and pretreatment algae drink is made.By pretreatment algae drink obtained It is carbonated with carbon dioxide so that carbon dioxide content is 0.55% to get to algae drink in drink.
Anti-oxidation function is tested
Detection method:According to superoxide dismutase (SOD) determination of activity in GB/T 5009.171-2003 health foods Method is measured the obtained algae drinks of above-described embodiment 1-4 and control sample water.Unit is detected in terms of bottle, bottled capacity is 500ml。
Testing result:It is shown in Table 1.
The anti-oxidation function test result of the obtained algae drinks of 1 embodiment 1-4 of table and control sample water
From the data in table 1, it can be seen that the preparation-obtained algae drink of the embodiment of the present invention has higher antioxidant activity, it can It is effectively retained the anti-oxidation function of algae itself.
Anticoagulant functions are tested
Detection method:It is obtained to above-described embodiment 1-4 according to Chinese Pharmacopoeia two annex, Ⅻ D heparin bioassary method Algae drink and control sample water are measured.
Testing result:It is shown in Table 2.
The anticoagulant functions test result of the obtained algae drinks of 2 embodiment 1-4 of table and control sample water
From the data in table 2, it can be seen that the preparation-obtained algae drink of the embodiment of the present invention has higher anticoagulant functions, it can It is effectively retained the anticoagulant functions of brown alga itself.
Flavor evaluation
Flavor evaluation measurement is carried out to the obtained algae drinks of above-described embodiment 1-4, the results are shown in Table 3.
The obtained algae drink flavor evaluation results of 3 embodiment 1-4 of table
As seen from the results in Table 3, algae drink provided by the invention is not only solved after using pure natural hops tea allotment The fishy smell problem of seaweed, also so that seaweed beverage mouthfeel it is clean, it is tasty and refreshing, have the slight fragrance of a flower, potableness preferable.

Claims (10)

1. a kind of algae drink preparation method based on marine microorganism ferment, which is characterized in that include the following steps:
The preparation of algae ferment:It will go out through shearing after fresh algae raw material or dry algae rehydration plus after water homogenisation in immigration fermentation tank Fermentation substrate after sterilizing is successively accessed bacillus subtilis suspension under different temperatures and lactobacillus plantarum suspension is sent out by bacterium Seaweed ferment after fermentation is obtained algae ferment by ferment by being separated by solid-liquid separation;
The preparation of hops tea:Pure water by hop pellets and after boiling is according to 1:The ratio of 100-150 is prepared, and is continued after preparation 1min is boiled, stops heating and being cooled to room temperature, hops tea is obtained by filtration;
Pre-process the preparation of algae drink:By mass fraction by algae ferment 1.5%-15%, hops tea 1%-10%, syrup 2%-5%, acid flavoring 0.01%-0.05%, surplus are to be stirred evenly in water input material-compound tank, obtain pretreatment algae drink;
Carbonating:Pretreatment algae drink is carbonated with carbon dioxide, obtains algae drink.
2. preparation method according to claim 1, which is characterized in that the algae includes red algae and brown alga, the algae Ferment is the mixture of red algae ferment and brown alga ferment, wherein the mass fraction of the red algae ferment of addition is 0.5-5%, brown alga The mass fraction of ferment is 1-10%.
3. preparation method according to claim 2, which is characterized in that the mass fraction of the red algae ferment of addition is 0.5%- 2%, the mass fraction of brown alga ferment is 2-5%, and the coefficient optimum proportion of hops tea is 2-6%.
4. preparation method according to claim 1, which is characterized in that carbon dioxide content is in the algae drink 0.35%-0.65%.
5. preparation method according to claim 1, which is characterized in that the fermentation substrate after sterilizing is pressed inoculum concentration 1.5% The sterile prepared bacillus subtilis suspension of access, ferments 3 days in 40 DEG C of stirrings, stops stirring, then cool the temperature to 30 DEG C, By the sterile prepared lactobacillus plantarum suspension of access of inoculum concentration 1%, is stirred 5 minutes in 30 DEG C, stop stirring, stationary culture 2 It.
6. preparation method according to claim 1, which is characterized in that the syrup is in fructose syrup or glucose syrup It is at least one.
7. preparation method according to claim 1, which is characterized in that the acid flavoring is citric acid, malic acid or lactic acid At least one of.
8. preparation method according to claim 1, which is characterized in that the hops are aroma hops or bitter fragrant inclusive type wine It spends, any one in Qingdao great Hua, Tettanger, Saaz, Hallertauer MFR, Cascade or Citral.
9. preparation method according to claim 2 or 3, which is characterized in that the red algae includes seaweed, agar, dragon At least one of dish, Irish moss or Eucheuma, the brown alga include at least one in kelp, kelp, undaria pinnitafida or sargassum fusifome Kind.
10. a kind of algae drink being prepared such as the claims 1-8 any one of them preparation methods.
CN201810174664.8A 2018-03-02 2018-03-02 A kind of algae drink preparation method and gained algae drink based on marine microorganism ferment Pending CN108432994A (en)

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CN103704839A (en) * 2013-12-18 2014-04-09 王桂宏 Production method for seaweed beverage
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Application publication date: 20180824