CN108426927A - Hematocrit is than computational methods, biochemical indicator data calibration method and correction system - Google Patents
Hematocrit is than computational methods, biochemical indicator data calibration method and correction system Download PDFInfo
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- CN108426927A CN108426927A CN201710080338.6A CN201710080338A CN108426927A CN 108426927 A CN108426927 A CN 108426927A CN 201710080338 A CN201710080338 A CN 201710080338A CN 108426927 A CN108426927 A CN 108426927A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
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Abstract
A kind of hematocrit in blood includes than computational methods：Apply blood sample in the reagent layer of Electrochemical Detection on piece；Sequentially apply the first high positive voltage and the second high positive voltage to blood sample, wherein the second high positive voltage is more than the first high positive voltage, and the first high positive voltage is more than or equal to 1.0 volts；Calculate charge value corresponding during applying the second high positive voltage；And the hematocrit ratio in blood sample is calculated according to charge value.
Description
Technical field
The invention relates to the hematocrits in a kind of blood than the biochemical indicator data calibration in computational methods, blood
Method and its correction system.
Background technology
In the detection process of whole blood, hematocrit ratio hematocrit, HCT in whole blood sample) often influence detected value.
By taking blood glucose as an example, when hematocrit than it is high when, detection blood glucose value can then underestimate.It is anti-, when hematocrit than it is low when, inspection
The blood glucose value of survey can then be over-evaluated.Most at present electrochemical sensor is all to overcome hematocrit ratio dense in blood glucose with alternating current
Interference on degree, but the test piece electrode design of adopting said method, majority needs 4 or 4 or more electrodes, and removes electrode design
It is upper complex outer, also because being detected simultaneously using AC impedance (AC impedance) and direct current, cause blood glucose meter
Circuit design can be more complex.
In addition to this, if being detected with direct current, existing sensor is mostly that one section of voltage applies, and calculates blood cell and hold
The method of product ratio is all obtaining current value.However, the method is often because of the interference of other substances in reagent layer or blood, and cause blood cell
Volumetric ratio measurement is extremely inaccurate, and its test piece procedure for producing also becomes not easy to control, and result is caused to be difficult to reproduce.
Therefore, a kind of bearing calibration to solve the above problems and correction system how to be proposed, is that current industry wants to throw
Enter development resources one of to solve the problems, such as.
Invention content
In view of this, the purpose of the present invention is the hematocrit being accurately calculated in blood sample ratio, and pass through
This hematocrit biochemical indicator numerical value more measured than in correction blood sample.
In order to achieve the above object, an embodiment according to the present invention, the hematocrit in a kind of blood is than calculating side
Method includes：Apply blood sample in the reagent layer of Electrochemical Detection on piece；Blood sample is sequentially applied the first high positive voltage with
And second high positive voltage, wherein the second high positive voltage is more than the first high positive voltage, and the first high positive voltage is more than or equal to 1.0 volts
It is special；Calculate charge value corresponding during applying the second high positive voltage；And the blood cell in blood sample is calculated according to charge value
Volumetric ratio.
It is above-mentioned to include according to charge value calculating hematocrit ratio in one or more embodiments of the present invention：With electricity
Magnitude is mapped than curve based on Standard clectrical quantity-hematocrit and obtains hematocrit ratio.
In one or more embodiments of the present invention, the first above-mentioned high positive voltage is 1.0~2.0 volts, and second
High positive voltage is 2.4~4.0 volts.
In one or more embodiments of the present invention, the application of the first above-mentioned high positive voltage continued for the first period, the
The application of two high positive voltages continued for the second period, and the second period was greater than or equal to for the first period.
In one or more embodiments of the present invention, the first above-mentioned period is 0.1~1.0 second, and the second period was
1.0~10 seconds.
In one or more embodiments of the present invention, interval period between the first period and the second period is 0~
10 seconds.
In order to achieve the above object, another embodiment according to the present invention, the biochemical indicator numerical value school in a kind of blood
Correction method includes sequentially：Apply blood sample in the reagent layer of Electrochemical Detection on piece；Low-voltage is applied to blood sample, to take
The original biochemical indicator of blood sample is obtained, the wherein absolute value of low-voltage is less than 1.0 volts；It is high just that first is applied to blood sample
Voltage, wherein the first high positive voltage is more than or equal to 1.0 volts；Second high positive voltage is applied to blood sample, wherein second is high just
Voltage is more than the first high positive voltage；Calculate charge value corresponding during applying the second high positive voltage；Blood is calculated according to charge value
Hematocrit ratio in liquid sample；And according to hematocrit than correcting original biochemical indicator numerical value.
It is above-mentioned to include according to charge value calculating hematocrit ratio in one or more embodiments of the present invention：With electricity
Magnitude is based on Standard clectrical quantity-hematocrit and calculates acquisition hematocrit ratio than relational expression.
It is above-mentioned according to hematocrit biochemical indicator numerical value more original than correction in one or more embodiments of the present invention
Including：It is based on standard correction value-hematocrit with hematocrit ratio and calculates acquisition corrected value than relational expression；And by original biochemistry
Index value is multiplied with corrected value.
In one or more embodiments of the present invention, the absolute value of above-mentioned low-voltage is 0.1~0.7 volt.
In one or more embodiments of the present invention, the sustained periods of time of the application of above-mentioned low-voltage is 1.0~10 seconds.
In order to achieve the above object, another embodiment according to the present invention, the biochemical indicator numerical value school in a kind of blood
Positive system includes Electrochemical Detection piece and processor.Electrochemical Detection piece includes base material, first electrode, second electrode and examination
Oxidant layer.Wherein first electrode connects processor anode, and second electrode connects processor cathode.First electrode is set to the surface of base material
On.Second electrode is set on this surface.Reagent layer is set on this surface, and at least partly covering first electrode and the second electricity
Pole.Processor is configured sequentially to apply low-voltage between first electrode and second electrode to obtain the original biochemistry of blood sample
Index applies the first high positive voltage and applies the second high positive voltage.The absolute value of low-voltage is less than 1.0 volts.First is high just
Voltage is more than or equal to 1.0 volts.Second high positive voltage is more than the first high positive voltage.Processor also configures high to calculate application second
Corresponding charge value during positive voltage, configuration with calculated according to charge value hematocrit ratio in blood sample and configuration with
According to hematocrit than correcting original biochemical indicator numerical value.
In one or more embodiments of the present invention, the material of above-mentioned first electrode includes in carbon, palladium, platinum and gold
At least one.
In one or more embodiments of the present invention, the material of above-mentioned second electrode includes in carbon, palladium, platinum and gold
At least one.
In one or more embodiments of the present invention, the line impedance summation of above-mentioned first electrode and second electrode is
300~1500 ohm.
In one or more embodiments of the present invention, above-mentioned reagent layer includes ferment and an electron transmission medium.
In one or more embodiments of the present invention, the contact area between above-mentioned second electrode and reagent layer is
0.8~1.2mm2。
In one or more embodiments of the present invention, there is the first contact surface between above-mentioned first electrode and reagent layer
Product.There is the second contact area, and the ratio between the second contact area and the first contact area between second electrode and reagent layer
Value is 1.0~2.0.
In one or more embodiments of the present invention, the thickness of above-mentioned first electrode and the thickness of second electrode are 6
~20 μm.
In conclusion the hematocrit in the blood of the present invention is by sequentially applying two groups of high positive electricity than computational methods
Pressure, and corresponding charge value calculates the ratio of the hematocrit in blood sample when by applying second group of high positive voltage.Due to
Standard clectrical quantity-hematocrit is very more linear than curve, therefore carries out mapping with charge value and can be obtained accurate blood cell appearance
Product ratio.Biochemical indicator data calibration method in the blood of the present invention and the biochemical indicator data calibration system in blood can lead to
Cross the calculated accurate hematocrit biochemical indicator numerical value more measured than in correction blood sample.Also, the present invention's
The electrode design of biochemical indicator data calibration system in blood is simple (only with two electrodes), and voltage applying mode is simple
(only applying DC voltage), and detection time is short.The biochemical indicator numerical value of blood sample described in this case can be blood sugar concentration or urine
Acid concentration or other can be by hematocrit than influencing biochemical indicator numerical value.
It is described above only illustrating the problem of present invention is to be solved, technical means to solve problem and its generation
Effect etc., detail of the invention will be discussed in detail in embodiment and relevant drawings below.
Description of the drawings
For above and other purpose, feature, advantage and the embodiment of the present invention can be clearer and more comprehensible, appended attached drawing is said
It is bright as follows：
Fig. 1 is the schematic diagram for the biochemical indicator data calibration system being painted in the blood of an embodiment of the present invention.
Fig. 2 is the sectional view of the Electrochemical Detection piece that is painted in Fig. 1 along line segment 2-2.
Fig. 3 is the flow chart of the hematocrit that is painted in the blood of an embodiment of the present invention than computational methods.
Fig. 4 is the voltage-vs-time of the application the first high positive voltage and the second high positive voltage that are painted an embodiment of the present invention
Curve graph.
Fig. 5 is the current-vs-time of the application the first high positive voltage and the second high positive voltage that are painted an embodiment of the present invention
Curve graph.
Fig. 6 is the electricity-hematocrit ratio song being painted corresponding to second high positive voltage of application of an embodiment of the present invention
Line chart.
Fig. 7 is to be painted being summed up using different line impedances from second electrode in first electrode for an embodiment of the present invention
Under condition, applies electricity-hematocrit corresponding to the second high positive voltage and compare curve graph.
Fig. 8 is that be painted arranging in pairs or groups using different-thickness in first electrode and second electrode for an embodiment of the present invention not collinear
Under the condition of the anti-sum total of roadlock, applies electricity-hematocrit corresponding to the second high positive voltage and compare curve graph.
Fig. 9 be painted an embodiment of the present invention second electrode and first electrode respectively with the contact area of reagent layer
Under condition using different ratios, applies electricity-hematocrit corresponding to the second high positive voltage and compare curve graph.
Figure 10 is the flow chart for the biochemical indicator data calibration method being painted in the blood of an embodiment of the present invention.
Figure 11 is the application low-voltage for being painted an embodiment of the present invention, the electricity of the first high positive voltage and the second high positive voltage
Pressure-time plot.
Figure 12 be painted an embodiment of the present invention after the biochemical indicator data calibration method in blood is corrected
Biochemical indicator numerical bias percentage-hematocrit compare test chart.
Figure 13 is to be painted the corrected value-hematocrit of an embodiment of the present invention to compare curve graph.
【Symbol description】
100：Biochemical indicator data calibration system in blood
110：Electrochemical Detection piece
111：Base material
112：First electrode
113：Second electrode
114：Reagent layer
120：Processor
A1：First contact area
A2：Second contact area
HCT：Hematocrit ratio
H1：First high positive voltage
H2：Second high positive voltage
L：Low-voltage
R1：First line impedance
R2：Second line impedance
T1：First thickness
T2：Second thickness
WB：Blood sugar concentration
S101~S207：Step
Specific implementation mode
Multiple embodiments of the present invention, as clearly stated, the details in many practices will be disclosed with attached drawing below
It will be explained in the following description.It should be appreciated, however, that the details in these practices is not applied to limit the present invention.Also
It is to say, in some embodiments of the present invention, the details in these practices is non-essential.In addition, for the sake of simplifying attached drawing, one
A little existing usual structures will be painted it in a manner of simply illustrating in the accompanying drawings with element.
Fig. 1 is please referred to, is the biochemical indicator data calibration system 100 being painted in the blood of an embodiment of the present invention
Schematic diagram.As shown in Figure 1, in present embodiment, the biochemical indicator data calibration system 100 in blood includes Electrochemical Detection
Piece 110 and processor 120.Please coordinate is the Electrochemical Detection piece 110 that is painted in Fig. 1 with reference to Fig. 2 along line segment 2-2
Sectional view.As shown in Figures 1 and 2, Electrochemical Detection piece 110 include base material 111, first electrode 112, second electrode 113 and
Reagent layer 114.First electrode 112, second electrode 113 and reagent layer 114 are set on the same surface of base material 111.Reagent layer
114 at least partly cover first electrode 112 and second electrode 113.Processor 120 is electrically connected first electrode 112 and the second electricity
Pole 113, and configure to form a potential difference between first electrode 112 and second electrode 113 (via reagent layer 114).Yu Ben
In embodiment, first electrode 112 connects processor anode, and second electrode 113 connects processor cathode.
In some embodiments, the material of first electrode 112 include in carbon, palladium, platinum and gold at least first, but this
Invention is not limited thereto.
In some embodiments, the material of second electrode 113 include in carbon, palladium, platinum and gold at least first, but this
Invention is not limited thereto.
Fig. 3 is please referred to, is the flow of the hematocrit that is painted in the blood of an embodiment of the present invention than computational methods
Figure.As shown in figure 3, the hematocrit in the blood of present embodiment can utilize the life in blood shown in FIG. 1 than computational methods
Change index value correction system 100 to implement.Hematocrit in the blood of present embodiment includes step S101 than computational methods
~S104.
First, in step S101, blood sample is applied in the reagent layer 114 on Electrochemical Detection piece 110.
Then, in step s 102, blood sample is sequentially applied the first high positive voltage and the second high positive voltage, wherein
Second high positive voltage is more than the first high positive voltage, and the first high positive voltage is more than or equal to 1.0 volts.It please coordinate with reference to Fig. 4, be
It is painted the voltage-time curve figure of application the first high positive voltage H1 and the second high positive voltage H2 of an embodiment of the present invention.
It should be noted that the purpose for forming a potential difference between first electrode 112 and second electrode 113 is to promote to try
Blood sample in oxidant layer 114 chemically reacts, and then generates electronics and transmit signal, i.e., so-called current value.
For example, in the embodiment for detecting blood glucose value using Electrochemical Detection piece 110, reagent layer 114 is wrapped
Glucose (Glucose) in blood sample can be converted to glucolactone by the main ingredient contained
(Gluconolactone), then by oxidation state electron transmission medium (Mediator) it is reduced into reduction-state substance.And it penetrates
The mode for applying potential difference between first electrode 112 and second electrode 113, can be by reduction-state substance reaction at oxidation state species.
Substance in reagent layer 114 includes the ferment that reaction can be accelerated to carry out, such as glucose dehydrogenase (Glucose
Dehydrogenase, GDH) ferment or glucose oxidase (Glucose oxidase, GOD) ferment, other are such as buffer solution
(Buffer), electron transmission medium (Mediator), ferment stabilizer, forming agent, interfacial agent or thickener etc..
In an embodiment, the first high positive voltage H1 is 1.0~2.0 volts, and preferably 1.2~1.8 volts.
In an embodiment, the second high positive voltage H2 is 2.4~4.0 volts and preferably 2.8~3.6 volts.
In an embodiment, the application of the first high positive voltage H1 continued for the first period, the application of the second high positive voltage H2
Continued for the second period, and the second period was greater than or equal to for the first period.For example, the first period was 0.1~1.0 second, and the
Two periods were 1.0~10 seconds.
As shown in figure 4, the first high positive voltage H1 and the second high positive voltage H2 that are applied are all definite value, but the present invention is not
As limit.
Also, in present embodiment, the interval period between the first period and the second period is preferably 0 second (also
Uninterruptedly apply that is, the second high positive voltage H2 connects the first high positive voltage H1).In an embodiment, the first period with
Interval period between second period is 0~10 second.
Then, in step s 103, corresponding charge value is calculated during applying the second high positive voltage H2.It please coordinate
With reference to Fig. 5, be painted an embodiment of the present invention application the first high positive voltage H1 and the second high positive voltage H2 electric current-when
Half interval contour figure.As shown in figure 5, (i.e. through the area under the current versus time curve calculated corresponding to the second high positive voltage H2 of application
Electricity), different hematocrit ratio HCT can be obtained and have difference results on area.
Fig. 6 is please referred to, is the electricity-blood cell being painted corresponding to second high positive voltage of application of an embodiment of the present invention
Volumetric ratio curve graph.Fig. 6 can apply the specific second high positive electricity respectively by multigroup blood sample with different hematocrit ratio HCT
It presses H2 and obtains.That is, electricity-hematocrit shown in fig. 6 can be considered than curve applies specific second high positive voltage H2
Corresponding Standard clectrical quantity-hematocrit compares curve.
In other embodiment, aforesaid standards electricity-hematocrit also can be exchanged into one Standard clectrical quantity-blood cell than curve
Volumetric ratio relational expression, as long as therefore the charge value for applying corresponding to specific second high positive voltage H2 is substituted into this relational expression, i.e.,
The hematocrit ratio HCT corresponding to this charge value can be found out.
It should be noted that some prior arts only apply one section of high positive voltage, and at the end of being applied with this high positive voltage
Current value estimates different hematocrit ratios.However, electric current-hematocrit for being made of these prior arts not than curve line
Property (also that is, same current value may be corresponded to two hematocrit ratios), therefore simultaneously can not effectively identify different blood
Ball volumetric ratio.In comparison, it can be understood by Fig. 6 of the present invention and be learnt, apply the electricity corresponding to specific second high positive voltage H2
Magnitude can generally increase with the increase of hematocrit ratio HCT, and charge value and hematocrit than relationship it is very linear
(being substantially proportionate), therefore can effectively identify different hematocrit ratio HCT.
Finally, in step S104, the hematocrit ratio HCT in blood sample is calculated according to charge value.As before
It is described, since the relationship of charge value and hematocrit ratio HCT is very linear, only it is to be understood that applying specific second high positive voltage
Corresponding charge value when H2, you can electricity-hematocrit is mapped than curve graph and obtained compared with subject to according to figure 6
True hematocrit ratio HCT.
In an embodiment, step S102~S104 can be executed by processor 120, but the present invention not as
Limit.
Fig. 7 is please referred to, is to be painted being used not in first electrode 112 and second electrode 113 for an embodiment of the present invention
Under condition with line impedance sum total, applies electricity-hematocrit corresponding to the second high positive voltage H2 and compare curve graph.Such as figure
1, with shown in Fig. 7, uses different in the first line impedance R1 of first electrode 112 and the second line impedance R2 of second electrode 113
Line impedance sum up (for example, 400ohm and 1000ohm) and arrange in pairs or groups different blood sugar concentration WB (for example, 200mg/dL and
Under condition 400mg/dL), apply electricity-hematocrit that specific second high positive voltage H2 is made remained to than curve it is clear
Ground identifies the difference of different hematocrit ratio HCT, and this difference is not by interfering with the blood sugar concentration WB for surveying blood sample.
Fig. 8 is please referred to, is to be painted being used not in first electrode 112 and second electrode 113 for an embodiment of the present invention
Stack pile is arranged in pairs or groups under the condition that different line impedances are summed up, and electricity-hematocrit corresponding to the second high positive voltage H2 is applied
Compare curve graph.As shown in Fig. 2 and Fig. 8, first electrode 112 and second electrode 113 be respectively adopted different first thickness T1 and
Second thickness T2, and the first line impedance R1 for first electrode 112 of arranging in pairs or groups and the second line impedance R2 of second electrode 113 are used
Under the condition of different line impedance sum totals, applies electricity-hematocrit that specific second high positive voltage H2 is made and compare curve
Remain to clearly identify the difference of different hematocrit ratio HCT.In present embodiment, the first of first electrode 112 is thick
It is 6~20 μm to spend T1 and the second thickness T2 of second electrode 113, preferably 10 μm~17 μm.
Please refer to Fig. 9, be painted an embodiment of the present invention second electrode 113 and first electrode 112 respectively with
The contact area of reagent layer 114 uses under the condition of different ratios, applies electricity-blood cell corresponding to the second high positive voltage H2
Volumetric ratio curve graph.As shown in Figure 1, having the first contact area A1 (the i.e. first electricity between first electrode 112 and reagent layer 114
The area that pole 112 is covered by reagent layer 114), and there is the second contact area A2 between second electrode 113 and reagent layer 114
(area that i.e. second electrode 113 is covered by reagent layer 114).As shown in figure 9, in the second contact area of second electrode 113
A2 is used from the first contact area A1 of first electrode 112 under the condition of different ratio, applies specific second high positive voltage
Electricity-hematocrit that H2 is made remains to clearly identify the difference of different hematocrit ratio HCT than curve.Yu Benshi
Apply in mode, the ratio between the second contact area A2 and the first contact area A1 be 1.33~1.63, but the present invention not with
This is limited.In practical application, the ratio between the second contact area A2 and the first contact area A1 can be 1.0~2.0.
In an embodiment, the contact area (i.e. the second contact area A2) between second electrode 113 and reagent layer 114
For 0.8~1.2mm2。
Figure 10 is please referred to, is the stream for the biochemical indicator data calibration method being painted in the blood of an embodiment of the present invention
Cheng Tu.As shown in Figure 10, the biochemical indicator data calibration method in the blood of present embodiment can utilize in blood shown in FIG. 1
Biochemical indicator data calibration system 100 implement.Biochemical indicator data calibration method in the blood of present embodiment is sequentially wrapped
S201 containing step~S207.
First, in step s 201, blood sample is applied in the reagent layer 114 on Electrochemical Detection piece 110.
In step S202, blood sample is applied in low-voltage, to obtain the original biochemical indicator of blood sample, low-voltage
Can be positive voltage or negative voltage, the wherein absolute value of low-voltage is less than 1.0 volts.
In an embodiment, the absolute value of low-voltage is 0.1~0.7 volt, and preferably 0.3~0.5 volt.
In an embodiment, the sustained periods of time of the application of low-voltage is 1.0~10 seconds.
In step S203, blood sample is applied in the first high positive voltage, wherein the first high positive voltage is more than or equal to 1.0 volts
It is special.
In step S204, blood sample is applied in the second high positive voltage, wherein the second high positive voltage is more than the first high electricity
Pressure.
Please coordinate referring to Fig.1 1, be the application low-voltage L for being painted an embodiment of the present invention, the first high positive voltage H1 with
The voltage-time curve figure of second high positive voltage H2.As shown in figure 11, the low-voltage L, the first high positive voltage H1 that are applied and
Two high positive voltage H2 are all definite value, but the present invention is not limited thereto.
In step S205, corresponding charge value is calculated during applying the second high positive voltage H2.
In step S206, the hematocrit ratio HCT in blood sample is calculated according to charge value.
It should be noted that related in biochemical indicator data calibration method in the blood of present embodiment calculate blood cell appearance
The step of step S203~S206 of the product than HCT, hematocrit ratio HCT computational methods in blood substantially as shown in figure 3
S102~S104 is identical, therefore can refer to aforementioned related description, herein without repeating.
Finally, in step S207, original biochemical indicator numerical value is corrected according to hematocrit ratio HCT.
Specifically, Figure 12 and Figure 13 is please referred to.Figure 12 be painted an embodiment of the present invention through the life in blood
Change biochemical indicator numerical bias percentage-hematocrit ratio HCT test charts after index value bearing calibration is corrected.Figure 13
Corrected value-hematocrit to be painted an embodiment of the present invention compares curve graph.In present embodiment, step S207 can be wrapped
Contain：It is based on standard correction value-hematocrit with hematocrit ratio HCT and calculates acquisition corrected value than relational expression；And by original life
Change index value to be multiplied with corrected value.In other words, corrected value is multiple proportional.In practical application, blood sample is applied
Standard correction value-hematocrit corresponding to specific second positive voltage is than the correction that relational expression can be obtained by repetition test
Value-hematocrit is converted than curve (as shown in figure 13), but the present invention is not limited thereto.
For example, in the embodiment for detecting blood glucose using Electrochemical Detection piece 110, (also that is, applying low-voltage L
Measured original biochemical indicator is blood glucose value), electrode design includes the following conditions：The First Line roadlock of first electrode 112
The line impedance sum total of anti-R1 and the second line impedance R2 of second electrode 113 is 800 ohm, second electrode 113 second connects
It is 0.9mm that the ratio that contacting surface accumulates A2 and the first contact area A1 of first electrode 112, which is the 1.5, second contact area A2,2, first
The first thickness T1 of electrode 112 is about 15 μm and the second thickness T2 of second electrode 113 is about 14 μm.
Under the condition of former electrodes design, to apply the current-vs-time figure corresponding to specific second high positive voltage H2
Area under a curve (i.e. electricity) compares Standard clectrical quantity-hematocrit shown in fig. 6 and obtains hematocrit ratio HCT than curve,
Then the hematocrit obtained ratio HCT is calculated into (or the correction by Figure 13 by standard correction value-hematocrit than relational expression
Value-hematocrit is mapped than curve graph and is obtained) corresponding corrected value, will finally apply current value that low-voltage L is obtained with
Numerical value after the multiplication of aforementioned corrected value is reconverted into blood glucose value, and (current value is also turned according to a particular kind of relationship formula with blood glucose value
Change, hold not describing herein), you can blood glucose value deviation measured under each hematocrit ratio HCT is eliminated, and then by blood sugar concentration
Deviation within correction to ± 10%.
In an embodiment, step S202~S207 can be executed by processor 120, but the present invention not as
Limit.
By above for the present invention specific implementation mode detailed description, it is apparent that the present invention blood in
Hematocrit is by sequentially applying two groups of high positive voltages than computational methods, and by applying second group of high positive voltage when is corresponding
Charge value calculate the ratio of the hematocrit in blood sample.Since Standard clectrical quantity-hematocrit is very more linear than curve,
Mapping, which is carried out, with charge value can be obtained accurate hematocrit ratio.Biochemical indicator data calibration in the blood of the present invention
Biochemical indicator data calibration system in method and blood can be by calculated accurate hematocrit than correction blood
Measured biochemical indicator numerical value in sample.Also, the electrode of the biochemical indicator data calibration system in the blood of the present invention is set
Meter is simple (only with two electrodes), and voltage applying mode is simple (only applying DC voltage), and detection time is short.Described in this case
The biochemical indicator numerical value of blood sample can be blood sugar concentration or uric acid concentration or other can be referred to by hematocrit than the biochemistry influenced
Mark numerical value.
Although the present invention is disclosed above with embodiment, so it is any to be familiar with this skill not to limit the present invention
Person, without departing from the spirit and scope of the present invention, when can be used for a variety of modifications and variations, therefore protection scope of the present invention is worked as
Subject to appended claims institute defender.
Claims (23)
1. the hematocrit in a kind of blood is than computational methods, including：
Apply a blood sample in a reagent layer of an Electrochemical Detection on piece；
One first high positive voltage and one second high positive voltage are sequentially applied to the blood sample, wherein second high positive voltage is big
In first high positive voltage, and first high positive voltage is more than or equal to 1.0 volts；
Calculate a charge value corresponding during applying second high positive voltage；And
The hematocrit ratio in the blood sample is calculated according to the charge value.
2. the hematocrit in blood as described in claim 1 is than computational methods, wherein this calculates the blood according to the charge value
Ball volumetric ratio includes：
It is mapped than curve based on one Standard clectrical quantity-hematocrit with the charge value and obtains the hematocrit ratio.
3. the hematocrit in blood as described in claim 1 is than computational methods, wherein first high positive voltage be 1.0~
2.0 volts, and second high positive voltage is 2.4~4.0 volts.
4. the hematocrit in blood as described in claim 1 is than computational methods, the application of wherein first high positive voltage is held
Continuous one first period, the application of second high positive voltage continued for one second period, and second period be greater than or equal to this first
Period.
5. the hematocrit in blood as claimed in claim 4, than computational methods, wherein first period is 0.1~1.0 second,
And second period is 1.0~10 seconds.
6. the hematocrit in blood as claimed in claim 4 is than computational methods, wherein first period and this second when
An interval period between section is 0~10 second.
7. a kind of biochemical indicator data calibration method in blood, includes sequentially：
Apply a blood sample in a reagent layer of an Electrochemical Detection on piece；
One low-voltage is applied to the blood sample, with obtain the blood sample one original biochemical indicator, the wherein low-voltage
Absolute value is less than 1.0 volts；
One first high positive voltage is applied to the blood sample, wherein first high positive voltage is more than or equal to 1.0 volts；
One second high positive voltage is applied to the blood sample, wherein second high positive voltage is more than first high positive voltage；
Calculate a charge value corresponding during applying second high positive voltage；
The hematocrit ratio in the blood sample is calculated according to the charge value；And
According to the hematocrit than correcting the original biochemical indicator numerical value.
8. the biochemical indicator data calibration method in blood as claimed in claim 7, wherein this should according to charge value calculating
Hematocrit ratio includes：
It is based on one Standard clectrical quantity-hematocrit with the charge value and obtains the hematocrit ratio than relational expression calculating.
9. the biochemical indicator data calibration method in blood as claimed in claim 7, wherein this compares school according to the hematocrit
Just the original biochemical indicator numerical value includes：
It is based on a standard correction value-hematocrit with the hematocrit ratio and calculates one corrected value of acquisition than relational expression；And
The original biochemical indicator numerical value is multiplied with the corrected value.
10. the biochemical indicator data calibration method in blood as claimed in claim 7, the absolute value of the wherein low-voltage are
0.1~0.7 volt.
11. the biochemical indicator data calibration method in blood as claimed in claim 7, wherein the one of the application of the low-voltage hold
The continuous period is 1.0~10 seconds.
12. the biochemical indicator data calibration method in blood as claimed in claim 7, wherein first high positive voltage are 1.0
~2.0 volts, and second high positive voltage is 2.4~4.0 volts.
13. the biochemical indicator data calibration method in blood as claimed in claim 7, the wherein application of first high positive voltage
Continued for one first period, the application of second high positive voltage continued for one second period, and second period be greater than or equal to this
One period.
14. the biochemical indicator data calibration method in blood as claimed in claim 13, wherein first period be 0.1~
1.0 seconds, and second period is 1.0~10 seconds.
15. the biochemical indicator data calibration method in blood as claimed in claim 7, wherein first period and this second
An interval period between period is 0~10 second.
16. the biochemical indicator data calibration system in a kind of blood, including：
One Electrochemical Detection piece, including：
One base material；
One first electrode is set on a surface of the base material；
One second electrode is set on the surface；And
One reagent layer is set on the surface, and at least partly covers the first electrode and the second electrode；And
One processor is configured sequentially to apply a low-voltage between the first electrode and the second electrode to obtain a blood sample
This original biochemical indicator applies one first high positive voltage and applies one second high positive voltage, and wherein the low-voltage is exhausted
1.0 volts are less than to value, which is more than or equal to 1.0 volts, and second high positive voltage is being more than first height just
Voltage,
The wherein processor is also configured to calculate a charge value corresponding during applying second high positive voltage, configuration with basis
The charge value calculate a hematocrit ratio in the blood sample and configuration with according to the hematocrit than the correction original life
Change index value.
17. the biochemical indicator data calibration system in blood as claimed in claim 16, wherein the material choosing of the first electrode
At least one in the group that free carbon, palladium, platinum, gold are formed.
18. the biochemical indicator data calibration system in blood as claimed in claim 16, wherein the material choosing of the second electrode
At least one in the group that free carbon, palladium, platinum, gold are formed.
19. the biochemical indicator data calibration system in blood as claimed in claim 16, the wherein first electrode with this second
One line impedance summation of electrode is 300~1500 ohm.
20. the biochemical indicator data calibration system in blood as claimed in claim 16, the wherein reagent layer include a ferment
With an electron transmission medium.
21. the biochemical indicator data calibration system in blood as claimed in claim 16, the wherein second electrode and the reagent
Contact area between layer is 0.8~1.2mm2。
22. the biochemical indicator data calibration system in blood as claimed in claim 16, the wherein first electrode and the reagent
Layer between have one first contact area, between the second electrode and the reagent layer have one second contact area, and this second
A ratio between contact area and first contact area is 1.0~2.0.
23. the biochemical indicator data calibration system in blood as claimed in claim 16, the wherein thickness of the first electrode with
The thickness of the second electrode is 6~20 μm.
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