CN108424857A - A kind of research method of simultaneous foster pattern scenedesmus culture for waste water fume treatment - Google Patents
A kind of research method of simultaneous foster pattern scenedesmus culture for waste water fume treatment Download PDFInfo
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- CN108424857A CN108424857A CN201810340119.1A CN201810340119A CN108424857A CN 108424857 A CN108424857 A CN 108424857A CN 201810340119 A CN201810340119 A CN 201810340119A CN 108424857 A CN108424857 A CN 108424857A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
Abstract
The present invention discloses a kind of research method of the simultaneous foster pattern scenedesmus culture for waste water fume treatment, mainly includes the following steps：Scenedesmus is inoculated with：Scenedesmus S.obliquus is inoculated in container, BBM culture mediums are added, inoculation volume is 10% (V inoculum/V culture mediums), is subsequently placed in light incubator and cultivates 10~15 days；Wastewater Pretreatment：Micro-filtration removes microorganism and suspended solid particles in waste water, and carries out 100~121 DEG C of high pressure sterilization processing；Basic culture experiment；And foster pattern culture experiment.Waste water flue gas is utilized and handles under this and foster pattern using scenedesmus, need not additionally add nutriment and can produce has cost-benefit biomass, is a kind of dual strategy of sustainable development.
Description
Technical field
The present invention relates to biological treatment waste water technology fields, and in particular to a kind of simultaneous foster pattern for waste water fume treatment
The research method of scenedesmus culture.
Background technology
In recent years, microalgae has obtained extensive concern, and is used for wastewater treatment, carbon dioxide discharge-reduction and bio-fuel life
The processes such as production[1].Microalgae is combined with existing wastewater treatment, Pollutants in Wastewater can be efficiently reduced and be carried out at the same time life
Substance produces.
Microalgae is CO2Seal a variety of advantages that provide up for safekeeping, photosynthetic efficiency can get height than terrestrial plant higher, biomass
The product of value.[2]High concentration CO is released from different industrial circles2Exhaust gas, can be used as a kind of CO of economy2Source, for micro-
The biomass of algae produces[3]。
And under the conditions of support, microalgae can absorb inorganic and organic substrates by synchronous breathing and photosynthesis, while real
Existing photoautotrophy and heterotrophic growth.[4]Food wastewater is rich in the nutriments such as nitrogen, phosphorus, calcium, iron, aluminium and total organic carbon, Ke Yizuo
For a kind of effective organic wastewater come realize microalgae biomass produce.[5]Therefore, using food wastewater and from industrial
High concentration CO2Mixotrophic cultivation pattern, can reach inexpensive benefit micro algae biomass production.
Most of research mainly utilizes CO at present2The production for carrying out biofuel feedstock is combined with municipal sewage, will be eaten
Product wastewater application is very limited in the research of industrial wastewater.
Invention content
It is an object of the invention to overcome defect existing for above-mentioned background technology, provide a kind of for waste water fume treatment
And the research method of foster pattern scenedesmus culture.
To solve technical problem mentioned above in the background art, technical scheme of the present invention:One kind being used for waste water flue gas
The research method of the simultaneous foster pattern scenedesmus culture of processing, includes the following steps：
1) scenedesmus is inoculated with：Scenedesmus S.obliquus is inoculated in container, BBM culture mediums are added, inoculation volume is 10%
(V inoculums/V culture mediums) is subsequently placed in light incubator and cultivates 10~15 days；
Condition of culture is：23~27 DEG C of temperature, white fluorescent illuminate 110~135 μm of ol.m2.s-1And it is passed through 0.5~
1.0L.min-1Filtration sterilization air conditions under cultivate, absorbance of the scenedesmus suspension in matrix culture medium be adjusted to 0.5~
1.0；
2) Wastewater Pretreatment：Micro-filtration removes microorganism and suspended solid particles in waste water, and carries out 100~121 DEG C of high pressures
Sterilization treatment[6]；
Wherein, waste water comes from secondary urban sewage MWW and food wastewater FW；
3) basic culture experiment：In BBM culture mediums, with 5% CO2Synthesis gas is cultivated under following condition of culture
S.obliquus scenedesmus 6~10 days；
Condition of culture：23~27 DEG C of temperature, white fluorescent illuminate 110~135 μm of ol.m2.s-1；
4) simultaneous foster pattern culture experiment：
(1) in aluminium means of press seals serum bottle, 300 milliliters of above-mentioned steps 2 are added) in sterilized processing secondary urban sewage
MWW is inoculated with 3~5% (VInoculum/VWaste water), it is aided with food wastewater FW and flue gas CO2；
(2) secondary urban sewage MWW filtrates are diluted with food wastewater FW, and the food wastewater FW bodies of 3 different levels are arranged
Product diluted concentration is the CO of 0.5,1,2% and 3 different levels2A concentration of 5,10,14.1%；
(3) 12 sewage solution are prepared altogether：Secondary urban sewage MWW and food wastewater FW volume ratios are 100:0 (MWW, point
Not in 5,10 and 14.1%CO2Under), 99.5:0.5 (FW0.5, in 5,10 and 14.1%CO2Under), 99:1 (FW1, in 5,10 and
14.1%CO2Under) and 98:2 (FW2, in 5,10 and 14.1% CO2Under)；
(5) the sewage solution is placed in inoculation bottle, is cultivated in 150rpm, 27 DEG C of water bath, white fluorescent shines
Bright intensity is 120 μm of ol.m2.s-1Culture 6 days.
Micro-filtration uses 0.2 μm of nylon in the step 2)[7]。
CO in the step 3)2Synthesis gas is by 5%CO2And 95%N2Composition.
Compared with prior art, the present invention has the advantage that：The present invention obtained secondary urban sewage by culture in 6 days
(MWW) it is diluted with 0.5~1% food wastewater (FW), the highest growth of scenedesmus is supported under 10~14.1% carbon dioxide
0.42~0.44g.L-1, TN nutrient removals are up to 21~22mg.L-1, lipid production rate is 10~11mg.L-1.d-1And carbon water
Production of chemicals rate is 13~16mg.L-1.d-1.Food wastewater is respectively by the aliphatic acid in scenedesmus, palmitic acid and oleic acid content
It is increased to 8% and 6%.Therefore, food wastewater and exhaust gas CO2Application scenedesmus can be promoted to grow, improve lipid/carbon aquation
Close the productivity and sewage treating efficiency of object.
Utilize and handle waste water flue gas under this and foster pattern using scenedesmus, need not additionally add nutriment and
Can produce has cost-benefit biomass, is a kind of dual strategy of sustainable development.
Description of the drawings
Fig. 1 is FW and three difference COs of the S.obliquus in different diluted concentrations2Schematic diagram is grown under concentration level：
(a) undiluted secondary urban sewage MWW is represented；
(b) the food wastewater FW0.5 that dilution volume is 0.5% is represented；
(c) the food wastewater FW1 that dilution volume is 1% is represented；
(d) the food wastewater FW2 that dilution volume is 2% is represented.
Fig. 2 is FW and three difference COs of the S.obliquus in different diluted concentrations2Total nitrogen (TN) under concentration level
Removal rate：
(a) undiluted secondary urban sewage MWW is represented；
(b) the food wastewater FW0.5 that dilution volume is 0.5% is represented：
(c) the food wastewater FW1 that dilution volume is 1% is represented；
(d) the food wastewater FW2 that dilution volume is 2% is represented.
Fig. 3 is FW and three difference COs of the S.obliquus in different diluted concentrations2Total phosphorus (TP) under concentration level
Removal rate：
(a) undiluted secondary urban sewage MWW is represented；
(b) the food wastewater FW0.5 that dilution volume is 0.5% is represented：
(c) the food wastewater FW1 that dilution volume is 1% is represented；
(d) the food wastewater FW2 that dilution volume is 2% is represented.
Fig. 4 is FW and three difference COs of the S.obliquus in different diluted concentrations2Fatty acid methyl ester under concentration level
(FAME) composition, wherein SFA represent saturated fatty acid, and MUFA represents monounsaturated fatty acids, and PUFA represents how unsaturated fat
Fat acid：
(a) undiluted secondary urban sewage MWW is represented；
(b) the food wastewater FW0.5 that dilution volume is 0.5% is represented；
(c) the food wastewater FW1 that dilution volume is 1% is represented；
(d) the food wastewater FW2 that dilution volume is 2% is represented
Fig. 5 is FW and three difference COs of the S.obliquus in different diluted concentrations2Carbohydrate under concentration level,
The content and productivity of lipid：
(a) undiluted secondary urban sewage MWW is represented；
(b) the food wastewater FW0.5 that dilution volume is 0.5% is represented：
(c) the food wastewater FW1 that dilution volume is 1% is represented；
(d) the food wastewater FW2 that dilution volume is 2% is represented
Specific implementation mode
Below by specific embodiments and the drawings, the present invention is further illustrated.The embodiment of the present invention is in order to more
So that those skilled in the art is more fully understood the present invention well, any limitation is not made to the present invention.
Embodiment 1
The typical group of the present embodiment flue gas becomes CO2：14.1%；CO:300ppm；NOX:207ppm；SOX:53ppm
Steps are as follows for the method for the present invention：
1) commercially available scenedesmus S.obliquus is inoculated in 2L cylindrical chambers, wherein the BBM culture mediums containing 1.7L,
Inoculation volume is 10% (VInoculum/VCulture medium), it is subsequently placed in light incubator and cultivates 10 days；Condition of culture is 23 DEG C of temperature, white
110 μm of ol.m of fluorescent illumination2.s-1And it is passed through 0.5L.min-1Filtration sterilization air conditions under cultivate, scenedesmus suspension matrix train
The absorbance supported in base is adjusted to 0.5.
BBM culture medium groups become shown in following table：
| Title | Concentration/(mg.L-1) | Title | Concentration/(mg.L-1) |
| NaNO3 | 250 | KH2PO4 | 175 |
| K2HPO4 | 75 | MgSO4·7H2O | 75 |
| CaCl2·2H2O | 25 | NaCl | 25 |
| EDTA | 50 | KOH | 31 |
| FeSO4·7H2O | 4.98 | H3BO3 | 11.42 |
| ZnSO4·7H2O | 8.82 | MnCl2 | 1.44 |
| MoO3 | 0.71 | CuSO4·5H2O | 1.57 |
| Co(NO3)2·6H2O | 0.49 |
2) pretreatment of waste water：Secondary urban sewage (MWW) and food wastewater (FW) are removed using 0.2 μm of nylon micro-filtration
Microorganism and suspended solid particles, and carry out 121 DEG C of high pressure sterilization processing.
Secondary urban sewage (MWW) is collected from municipal sewage plant, and food wastewater (FW) comes from food wastewater treatment plant.
TN=21 ± the 0.4mg/L, TP=2.3 ± 0.2mg/L, TOC=18.6 ± 0.2mg/ of secondary urban sewage (MWW)
L；
TN=725 ± the 1.6mg/L, TP=62.5 ± 1.1mg/L, TOC=10173 ± 2.3mg/L of food wastewater (FW)；
3) basic culture experiment：In BBM culture mediums, with 5% CO2Synthesis gas (includes 5%CO2And 95%N2Group
Point) S.obliquus scenedesmus is cultivated under following condition of culture 6 days；
Condition of culture：23 DEG C of temperature, white fluorescent illuminate 110 μm of ol.m2.s-1；
4) simultaneous foster pattern culture experiment：
A, in 500 milliliters of aluminium means of press seals serum bottles, the secondary urban sewage MWW of 300 milliliters of filtration sterilization processing is added,
It is inoculated with 3% (VInoculum/VWaste water), it is aided with food wastewater FW and flue gas CO2；
B, MWW filtrates are diluted with FW, and the FW volume dilutions concentration 0.5,1,2% and 3 differences of 3 different levels is arranged
Horizontal CO2Concentration 5,10,14.1%；
C, 12 sewage solution are shared：Municipal sewage MWW and diluent FW volume ratios 100:0 (MWW, respectively in 5,10 and
14.1%CO2Under), 99.5:0.5 (FW0.5, in 5,10 and 14.1%CO2Under), 99:1 (FW1, in 5,10 and 14.1% CO2
Under) and 98:2 (FW2, in 5,10 and 14.1% CO2Under)；
D, above-mentioned sewage solution is placed in inoculation bottle, is cultivated in 150rpm, 27 DEG C of water bath, white fluorescent illumination
Intensity is 120 μm of ol.m2.s-1Culture 6 days.
Embodiment 2
The typical group of the present embodiment flue gas becomes CO2：14.1%；CO:300ppm；NOX:207ppm；SOX:53ppm
Steps are as follows for the method for the present invention：
1) commercially available scenedesmus S.obliquus is inoculated in 2L cylindrical chambers, wherein the BBM culture mediums containing 1.7L,
Inoculation volume is 10% (VInoculum/VCulture medium), it is subsequently placed in light incubator and cultivates 15 days；Condition of culture is 27 DEG C of temperature, white
135 μm of ol.m of fluorescent illumination2.s-1And it is passed through 1.0L.min-1Filtration sterilization air conditions under cultivate, scenedesmus suspension matrix train
The absorbance supported in base is adjusted to 1.0.
BBM culture mediums are formed with embodiment 1
2) pretreatment of waste water：Secondary urban sewage (MWW) and food wastewater (FW) are removed using 0.2 μm of nylon micro-filtration
Microorganism and suspended solid particles, and carry out 100 DEG C of high pressure sterilization processing.
Secondary urban sewage (MWW) is collected from municipal sewage plant, and food wastewater (FW) comes from food wastewater treatment plant.
TN=21 ± the 0.4mg/L, TP=2.3 ± 0.2mg/L, TOC=18.6 ± 0.2mg/ of secondary urban sewage (MWW)
L；
TN=725 ± the 1.6mg/L, TP=62.5 ± 1.1mg/L, TOC=10173 ± 2.3mg/L of food wastewater (FW)；
3) basic culture experiment：In BBM culture mediums, with 5% CO2Synthesis gas (includes 5%CO2And 95%N2Group
Point) S.obliquus scenedesmus is cultivated under following condition of culture 10 days；
Condition of culture：27 DEG C of temperature, white fluorescent illuminate 135 μm of ol.m2.s-1；
4) simultaneous foster pattern culture experiment：
A, in 500 milliliters of aluminium means of press seals serum bottles, the secondary urban sewage MWW of 300 milliliters of filtration sterilization processing is added,
It is inoculated with 5% (VInoculum/VWaste water), it is aided with food wastewater FW and flue gas CO2；
B, MWW filtrates are diluted with FW, and the FW volume dilutions concentration 0.5,1,2% and 3 differences of 3 different levels is arranged
Horizontal CO2Concentration 5,10,14.1%；
C, 12 sewage solution are shared：Municipal sewage MWW and diluent FW volume ratios 100:0 (MWW, respectively in 5,10 and
14.1%CO2Under), 99.5:0.5 (FW0.5, in 5,10 and 14.1%CO2Under), 99:1 (FW1, in 5,10 and 14.1% CO2
Under) and 98:2 (FW2, in 5,10 and 14.1% CO2Under)；
D, above-mentioned sewage solution is placed in inoculation bottle, is cultivated in 150rpm, 27 DEG C of water bath, white fluorescent illumination
Intensity is 120 μm of ol.m2.s-1Culture 6 days.
Embodiment 3
The typical group of the present embodiment flue gas becomes CO2：14.1%；CO:300ppm；NOX:207ppm；SOX:53ppm
Steps are as follows for the method for the present invention：
1) commercially available scenedesmus S.obliquus is inoculated in 2L cylindrical chambers, wherein the BBM culture mediums containing 1.7L,
Inoculation volume is 10% (VInoculum/VCulture medium), it is subsequently placed in light incubator and cultivates 13 days；Condition of culture is 25 DEG C of temperature, white
125 μm of ol.m of fluorescent illumination2.s-1And it is passed through 0.75L.min-1Filtration sterilization air conditions under cultivate, scenedesmus suspension is in matrix
Absorbance in culture medium is adjusted to 0.8.
BBM culture mediums are formed with embodiment 1
2) pretreatment of waste water：Secondary urban sewage (MWW) and food wastewater (FW) are removed using 0.2 μm of nylon micro-filtration
Microorganism and suspended solid particles, and carry out 115 DEG C of high pressure sterilization processing.
Secondary urban sewage (MWW) is collected from municipal sewage plant, and food wastewater (FW) comes from food wastewater treatment plant.
TN=21 ± the 0.4mg/L, TP=2.3 ± 0.2mg/L, TOC=18.6 ± 0.2mg/ of secondary urban sewage (MWW)
L；
TN=725 ± the 1.6mg/L, TP=62.5 ± 1.1mg/L, TOC=10173 ± 2.3mg/L of food wastewater (FW)；
3) basic culture experiment：In BBM culture mediums, with 5% CO2Synthesis gas (includes 5%CO2And 95%N2Group
Point) S.obliquus scenedesmus is cultivated under following condition of culture 8 days；
Condition of culture：25 DEG C of temperature, white fluorescent illuminate 125 μm of ol.m2.s-1；
4) simultaneous foster pattern culture experiment：
A, in 500 milliliters of aluminium means of press seals serum bottles, the secondary urban sewage MWW of 300 milliliters of filtration sterilization processing is added,
It is inoculated with 4% (VInoculum/VWaste water), it is aided with food wastewater FW and flue gas CO2；
B, MWW filtrates are diluted with FW, and the FW volume dilutions concentration 0.5,1,2% and 3 differences of 3 different levels is arranged
Horizontal CO2Concentration 5,10,14.1%；
C, 12 sewage solution are shared：Municipal sewage MWW and diluent FW volume ratios 100:0 (MWW, respectively in 5,10 and
14.1%CO2Under), 99.5:0.5 (FW0.5, in 5,10 and 14.1%CO2Under), 99:1 (FW1, in 5,10 and 14.1%CO2Under)
With 98:2 (FW2, in 5,10 and 14.1% CO2Under)；
D, above-mentioned sewage solution is placed in inoculation bottle, is cultivated in 150rpm, 27 DEG C of water bath, white fluorescent illumination
Intensity is 120 μm of ol.m2.s-1Culture 6 days.
Fig. 1 is FW and three difference COs of the S.obliquus in different diluted concentrations2Schematic diagram is grown under concentration level；
Fig. 2 is FW and three difference COs of the S.obliquus in different diluted concentrations2Total nitrogen (TN) under concentration level
Removal rate；
Fig. 3 is FW and three difference COs of the S.obliquus in different diluted concentrations2Total phosphorus (TP) under concentration level
Removal rate；
Fig. 4 is FW and three difference COs of the S.obliquus in different diluted concentrations2Fatty acid methyl ester under concentration level
(FAME) composition, wherein SFA represent saturated fatty acid, and MUFA represents monounsaturated fatty acids, and PUFA represents how unsaturated fat
Fat acid；
By Fig. 1 to Fig. 4 it can be seen that being aided with food wastewater FW and being passed through C02The simultaneous foster pattern condition of culture of gas
Under, be conducive to the growth of scenedesmus.Wherein, the food wastewater FW0.5 and FW1.0 that scenedesmus is 0.5% and 1% in dilution volume
The middle CO cultivated and be passed through 10% and 14.1%2Growing state is best after gas.And foster pattern culture scenedesmus is for TN nutrition
Removal effect is preferable, food wastewater FW0.5 and FW1.0 and be passed through 10% and 14.1% CO2Gas culture can reach highest
TN remove 21-22mg.L-1, and it is relatively low that scenedesmus removal TN is cultivated in MWW and FW2.Scenedesmus exists for the removal of TP
It is not much different under each condition of culture, can obtains preferable removal effect.In food wastewater FW0.5 and FW1.0 and it is passed through
10% and 14.1% CO2Scenedesmus can reach highest lipid production rate 10-11mg.L under gas condition of culture-1.d-1And carbon water
Production of chemicals rate 13-16mg.L-1.d-1.Meanwhile food wastewater is respectively by the aliphatic acid in scenedesmus, palmitic acid and oleic acid content
It is increased to 8% and 6%.Utilize food wastewater and CO2The simultaneous foster pattern of flue gas is conducive to the culture of scenedesmus, obtains higher
Lipid and carbohydrate productivity also achieve the purified treatment of waste water simultaneously.
It should be understood that embodiment and example discussed herein simply to illustrate that, to those skilled in the art
For, it can be improved or converted, and all these modifications and variations should all belong to the protection of appended claims of the present invention
Range.
Pertinent literature：
[1].Bhatnagar,A.,Chinnasamy,S.,Singh,M.,Das,K.C.,2011.Renewable
biomass production by mixotrophic algae in the presence of various carbon
sources and wastewaters.Appl.Energ.88, 3425-3431.
[2].Lizzul,A.M.,Hellier,P.,Purton,S.,Baganz,F.,Ladommatos,N.,Campos,
L.,2014.Combined remediation and lipid production using Chlorella sorokiniana
grown on wastewater and exhaust gases.Bioresour.Technol.151,12-18.
[3].Chiu,S.Y.,Kao,C.Y.,Huang,T.T.,Lin,C.J.,Ong,S.C.,Chen,C.D.,Chang,
J.S.,Lin, C.S.,2011.Microalgal biomass production and on-site bioremediation
of carbon dioxide,nitrogen oxide and sulfur dioxide from flue gas using
Chlorella sp.cultures.Bioresour.Technol.102, 9135-9142.
[4].Salama,E.-S.,Kabra,A.N.,Ji,M.-K.,Kim,J.R.,Min,B.,Jeon,B.-H.,
2014.Enhancement of microalgae growth and fatty acid content under the
influence of phytohormones.Bioresour.Technol. 172,97-103.
[5].Cohen,Y.,Kirchmann,H.,2004.Increasing the pH of wastewater to
high levels with different gases-CO2 stripping.Water Air Soil Poll.159,265-
275.
[6].Zhang,L.,Jahng,D.,2010.Enhanced anaerobic digestion of piggery
wastewater by ammonia stripping:effects of alkali types.J.Hazard.Mater.182,
536-543.
[7].Hu,B.,Min,M.,Zhou,W.,Du,Z.,Mohr,M.,Chen,P.,Zhu,J.,Cheng,Y.,Liu,
Y.,Ruan, R.,2012.Enhanced mixotrophic growth of microalga Chlorella sp.on
pretreated swine manure for simultaneous biofuel feedstock production and
nutrient removal.Bioresour.Technol.126,71-79.
Claims (3)
1. a kind of research method of simultaneous foster pattern scenedesmus culture for waste water fume treatment, which is characterized in that including walking as follows
Suddenly：
1) scenedesmus is inoculated with：Scenedesmus S.obliquus is inoculated in container, BBM culture mediums are added, inoculation volume is that 10% (V connects
Kind object/V culture mediums), it is subsequently placed in light incubator and cultivates 10~15 days；
Condition of culture is：23~27 DEG C of temperature, white fluorescent illuminate 110~135 μm of ol.m2.s-1And it is passed through 0.5~1.0L.min-1Filtration sterilization air conditions under cultivate, absorbance of the scenedesmus suspension in matrix culture medium is adjusted to 0.5~1.0；
2) Wastewater Pretreatment：Micro-filtration removes microorganism and suspended solid particles in waste water, and carries out 100~121 DEG C of high pressure sterilizations
Processing；
Wherein, waste water comes from secondary urban sewage and food wastewater；
3) basic culture experiment：In BBM culture mediums, with 5% CO2Synthesis gas is cultivated under following condition of culture
S.obliquus scenedesmus 6~10 days；
Condition of culture：23~27 DEG C of temperature, white fluorescent illuminate 110~135 μm of ol.m2.s-1；
4) simultaneous foster pattern culture experiment：
(1) in aluminium means of press seals serum bottle, 300 milliliters of above-mentioned steps 2 are added) in sterilized processing secondary urban sewage, connect
3~5% (V of kindInoculum/VWaste water), it is aided with food wastewater and flue gas CO2；
(2) secondary urban sewage filtrate is diluted with food wastewater, and the food wastewater volume dilution that 3 different levels are arranged is a concentration of
0.5, the CO of 1,2% and 3 different levels2A concentration of 5,10,14.1%；
(3) 12 sewage solution are prepared altogether：Secondary urban sewage and food wastewater volume ratio are 100:0 (secondary urban sewage, point
Not in 5,10 and 14.1%CO2Under), 99.5:0.5 (food wastewater 0.5, in 5,10 and 14.1%CO2Under), 99:1 (food wastewater
1, in 5,10 and 14.1%CO2Under) and 98:2 (food wastewaters 2, in 5,10 and 14.1% CO2Under)；
(5) the sewage solution is placed in inoculation bottle, is cultivated in 150rpm, 27 DEG C of water bath, white fluorescent illumination is strong
Degree is 120 μm of ol.m2.s-1Culture 6 days.
2. a kind of research method of simultaneous foster pattern scenedesmus culture for waste water fume treatment according to claim 1,
It is characterized in that, micro-filtration uses 0.2 μm of nylon in the step 2).
3. a kind of research method of simultaneous foster pattern scenedesmus culture for waste water fume treatment according to claim 1,
It is characterized in that, CO in the step 3)2Synthesis gas is by 5%CO2And 95%N2Composition.
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| CN109456895A (en) * | 2018-10-23 | 2019-03-12 | 天津大学 | A kind of CO that chemical absorbing is coupled with bioconversion2Capture method |
| CN109609383A (en) * | 2018-12-11 | 2019-04-12 | 天津大学 | Regulate and control and utilize NH based on pH4HCO3The microalgae culture method of absorbing liquid |
| CN109897784A (en) * | 2019-02-27 | 2019-06-18 | 天津大学 | A kind of method that novel two stages autotrophy-Heterotrophic culture promotes microalgae lipid |
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| CN109456895A (en) * | 2018-10-23 | 2019-03-12 | 天津大学 | A kind of CO that chemical absorbing is coupled with bioconversion2Capture method |
| CN109609383A (en) * | 2018-12-11 | 2019-04-12 | 天津大学 | Regulate and control and utilize NH based on pH4HCO3The microalgae culture method of absorbing liquid |
| CN109897784A (en) * | 2019-02-27 | 2019-06-18 | 天津大学 | A kind of method that novel two stages autotrophy-Heterotrophic culture promotes microalgae lipid |
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