CN108414513A - Alcohol and drug test All-in-One sampling detector in DNA sample acquisition, saliva - Google Patents
Alcohol and drug test All-in-One sampling detector in DNA sample acquisition, saliva Download PDFInfo
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- CN108414513A CN108414513A CN201810361169.8A CN201810361169A CN108414513A CN 108414513 A CN108414513 A CN 108414513A CN 201810361169 A CN201810361169 A CN 201810361169A CN 108414513 A CN108414513 A CN 108414513A
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- 210000003296 saliva Anatomy 0.000 title claims abstract description 89
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title claims abstract description 73
- 238000005070 sampling Methods 0.000 title claims abstract description 48
- 238000003255 drug test Methods 0.000 title claims abstract description 31
- 238000001514 detection method Methods 0.000 claims abstract description 97
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000012360 testing method Methods 0.000 claims description 50
- 239000003814 drug Substances 0.000 claims description 32
- 229940079593 drug Drugs 0.000 claims description 30
- 239000000463 material Substances 0.000 claims description 20
- BQJCRHHNABKAKU-KBQPJGBKSA-N morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 claims description 16
- 239000002117 illicit drug Substances 0.000 claims description 13
- 238000007689 inspection Methods 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- 239000000020 Nitrocellulose Substances 0.000 claims description 11
- 230000021615 conjugation Effects 0.000 claims description 11
- 239000010931 gold Substances 0.000 claims description 11
- 229910052737 gold Inorganic materials 0.000 claims description 11
- 229920001220 nitrocellulos Polymers 0.000 claims description 11
- 210000000981 epithelium Anatomy 0.000 claims description 10
- YQEZLKZALYSWHR-UHFFFAOYSA-N Ketamine Chemical compound C=1C=CC=C(Cl)C=1C1(NC)CCCCC1=O YQEZLKZALYSWHR-UHFFFAOYSA-N 0.000 claims description 8
- 229960003299 ketamine Drugs 0.000 claims description 8
- 229960005181 morphine Drugs 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 8
- 238000010521 absorption reaction Methods 0.000 claims description 7
- 230000000890 antigenic effect Effects 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 claims description 7
- 229960001252 methamphetamine Drugs 0.000 claims description 6
- 230000000007 visual effect Effects 0.000 claims description 4
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- 241001494479 Pecora Species 0.000 claims description 3
- 125000003158 alcohol group Chemical group 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 3
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- 239000000203 mixture Substances 0.000 claims description 3
- 229920001778 nylon Polymers 0.000 claims description 3
- 241000532370 Atla Species 0.000 claims description 2
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- 230000008859 change Effects 0.000 claims description 2
- 231100000252 nontoxic Toxicity 0.000 claims description 2
- 230000003000 nontoxic effect Effects 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- 239000003365 glass fiber Substances 0.000 claims 1
- 230000003533 narcotic effect Effects 0.000 abstract description 13
- 238000000034 method Methods 0.000 abstract description 7
- 206010011409 Cross infection Diseases 0.000 abstract description 2
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 2
- 230000006957 competitive inhibition Effects 0.000 abstract description 2
- 238000012252 genetic analysis Methods 0.000 abstract description 2
- 238000003317 immunochromatography Methods 0.000 abstract description 2
- 238000010339 medical test Methods 0.000 abstract description 2
- 210000000214 mouth Anatomy 0.000 description 13
- 239000008280 blood Substances 0.000 description 10
- 210000004369 blood Anatomy 0.000 description 10
- 238000010586 diagram Methods 0.000 description 10
- 210000003128 head Anatomy 0.000 description 6
- 239000002574 poison Substances 0.000 description 6
- 231100000614 poison Toxicity 0.000 description 6
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- -1 polyethylene Polymers 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- WBODDOZXDKQEFS-UHFFFAOYSA-N 1,2,3,4-tetramethyl-5-phenylbenzene Chemical group CC1=C(C)C(C)=CC(C=2C=CC=CC=2)=C1C WBODDOZXDKQEFS-UHFFFAOYSA-N 0.000 description 1
- 102000007698 Alcohol dehydrogenase Human genes 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 208000007848 Alcoholism Diseases 0.000 description 1
- 102000008102 Ankyrins Human genes 0.000 description 1
- 108010049777 Ankyrins Proteins 0.000 description 1
- 206010006326 Breath odour Diseases 0.000 description 1
- 206010013654 Drug abuse Diseases 0.000 description 1
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- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
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- 239000011521 glass Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 210000000653 nervous system Anatomy 0.000 description 1
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- 229920002223 polystyrene Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
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- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 208000011117 substance-related disease Diseases 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/0045—Devices for taking samples of body liquids
- A61B10/0051—Devices for taking samples of body liquids for taking saliva or sputum samples
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B10/00—Other methods or instruments for diagnosis, e.g. instruments for taking a cell sample, for biopsy, for vaccination diagnosis; Sex determination; Ovulation-period determination; Throat striking implements
- A61B10/02—Instruments for taking cell samples or for biopsy
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Heart & Thoracic Surgery (AREA)
- Public Health (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Medical Informatics (AREA)
- Hematology (AREA)
- Pulmonology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Analytical Chemistry (AREA)
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- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides alcohol and drug test All-in-One sampling detector in a kind of novel DNA sample acquisition, saliva, is made of sample detecting stick and drug test card, the sample detecting stick is for saliva, DNA sample acquisition and alcohol detection.The detection card that can be used for drug test in saliva sample is prepared the present invention is based on colloidal gold A competitive inhibition method immunochromatography technique.The All-in-One sampling detector of the present invention is easy to use, easy to operate, can complete DNA sample while narcotic saliva detects and collect, saliva alcohol content detects three indexs.This product is simple in structure, compact, is not only suitable for onsite application when law enforcement, is also very suitable for personal and family and uses at any time on demand.Realize painless acquisition, without any wound or invasive, user not will produce any discomfort sense.It is disposable, cross-infection risk is not present.The DNA sample acquired is suitable for genetic analysis detection and other medical tests etc..
Description
Technical field
The present invention relates to medical instruments fields, specifically, being related to alcohol and drug test in DNA sample acquisition, saliva
All-in-One sampling detector.
Background technology
With the rapid development of Chinese Automobile Industry ' and the raising of living standards of the people, motor vehicle and fresh driver are rapid
Increase.Present many traffic accidents are related to driver's driving with poison, drunk driving and drunk driving.Drug abuse can cause driver to generate
Phonism photis and nervous system is seriously affected, often results in very serious and great traffic accident.Driving with poison is China's explicit order
Forbid.In addition to driving with poison, in many systems, such as enlisted in the arms or civil servant's registration physical examination etc. also all proposes the need of illicit drugs inspection
It asks.Equally contain the poison with blood equivalent other than it can be detected by urine, blood or hair equal samples, in saliva
Product molecule can be used for the detection and screening of addicts.According to standard GB/T 19522-2010《Vehicle operator's blood,
Breath alcohol concentration threshold value and inspection》It is to drive when intoxicated that content, which reaches 20mg/100ml, in regulation driver's blood, in blood
It is drink-driving that content, which reaches 80mg/100ml,.Existing frequently-used blood testing needs professional and equipment, process
Complexity can not complete Site Detection.It is alcohol content detector when exhaling that law enforcement field, which is applied at most, and instrument price is high
Expensive, testing result is by the influences such as environment and driver's expiration mode, it is difficult to accurate quantification.The study found that alcohol in saliva
Concentration is close with blood alcohol concentration content, can completely by saliva detection come directly, fast and accurately reflect blood in wine
The content of essence.
The difficulty of acquisition urine specimen is avoided using saliva detection and privacy concern and authenticity are difficult to be completely secured
The problem of.Also avoid acquisition blood sample need professional, special environment and and special installation detect the problem of, operation
It is simple and convenient, it is very suitable for field quick detection.Meanwhile in practical enforcing law, it is due to being difficult to intuitive judgment driver
Drunk driving or driving with poison either way exist, and generally require the double check that drunk driving and driving with poison are carried out to driver.In addition,
For drunk driving driver, the driver especially to cause a serious accident has the necessity for acquiring its DNA sample, due to individual difference,
Everyone is larger to the metabolic capability gap of alcohol, and in addition to alcohol detection, the crowd for having demand, such as alcohol generation are detected to other
Thank gene, the detection of alcohol tolerance gene etc., it is also desirable to a kind of mode that is easy, noninvasive, being easy to receiving be provided and obtain its DNA sample
Product.Therefore, exploitation one kind can realize that the integrated products of drugs in saliva, alcohol detection and DNA sample acquisition are aobvious
It obtains particularly necessary.
Invention content
The object of the present invention is to provide alcohol and drug test All-in-One sample detectings in a kind of acquisition of DNA sample, saliva
Device.
It is a further object of the present invention to provide the applications of above-mentioned multi-functional sampling detector.
In order to realize the object of the invention, alcohol and drug test All-in-One in DNA sample acquisition provided by the invention, saliva
Sampling detector is made of sample detecting stick and drug test card, the sample detecting stick for saliva, DNA sample acquisition and
Alcohol detection.
The sample detecting stick includes grip part, sampling unit and test section；Wherein, the sampling sites are in the grip part
One end, and be connected with the test section being arranged on the grip part；It is fixed in the sampling unit for adsorbing saliva sample
This sorbing material is provided with alcohol detection test strips on the test section.
The present invention carries out the detection of drug in saliva sample using colloidal gold A competitive inhibition method immunochromatography technique.The medicine
Analyte detection card includes sample pad, gold conjugation pad, the nitrocellulose filter and absorption pad for being fixed with drug antigenic to be measured, described
Be fixed with drug antigenic to be measured nitrocellulose filter be equipped with several detection lines and at least 1 nature controlling line, the sample pad,
Gold conjugation pad, the nitrocellulose filter for being fixed with drug antigenic to be measured and absorption pad are pasted onto on plastics lining board successively.Its
In, the gold conjugation pad is fixed with the specific antibody of anti-drug to be measured, and it is polyclonal that the nature controlling line is fixed with sheep anti mouse
Antibody.
Specific antibody of the present invention includes monoclonal antibody.Therefrom select the highest monoclonal of sensitivity and specificity
Antibody is marked as drug antigenic, reaches the particular/special requirement of saliva detection.
Sample detecting stick above-mentioned, one end that the grip part is connected with the sampling unit are hollow tubular structure, are passed through
The saliva sample of fixed sorbing material acquisition enters alcohol detection test strips via hollow collecting pipe in sampling unit.
One end setting of sample detecting stick above-mentioned, the grip part is fluted, and the alcohol detection test strips are fixed on
In the groove.
The alcohol detection test strips groove position of sample detecting stick above-mentioned, the test section directly exposes or with transparent
Visual windows, the visual windows are corresponding with the position of alcohol detection test strips, for observing the aobvious of alcohol detection test strips
Colour response.
Sorbing material of the present invention is spongy top or other nontoxic hygroscopic materials.Preferably, in the spongy top
On be coated with nylon membrane, either tightened in the sampling unit adjacent spongy top the micro- tooth scraper plate nylon membrane of the subsidiary circle in region or
The design of micro- tooth scraper plate is for the ease of acquisition port intracavitary side mucosa cells and epithelial tissue.
Material for making alcohol detection test strips of the present invention be filter membrane or nitrocellulose filter etc. it is adsorbable and
The material of ankyrin, coated detection reagent is alcohol dehydrogenase and related colour reagent (tetramethyl biphenyl in test strips
Amine, a concentration of 50-500 μ g/ml), peridium concentration is 0.1-1 μ g/ml.The detection range of alcohol content is 0- in saliva sample
30%.
The other end of sample detecting stick above-mentioned, the grip part is anti-skidding design.
Preferably, drug test card of the present invention is additionally provided with a lug boss, the boss in the top of sample pad,
The center of lug boss is set there are one well, the size of well and fixed sorbing material size phase on the sample detecting stick
It matches, the saliva acquired on sorbing material enters sample pad by well.
The drug test card is additionally provided with visible observation window, which is located at the upper of the detection line and nature controlling line
Side is used for drug test result interpretation.In addition, medicine corresponding with drug test line to be measured (T lines) can be marked on visible observation window
Name claims, and the corresponding position of nature controlling line (C lines) can also be marked out on the visible observation window.Meanwhile in visible observation window
On blank space can be equipped with sample message register column.
The material of sample pad is cellulose membrane in drug test card of the present invention, and the material of the gold conjugation pad is glass
Tunica fibrosa.
Drug of the present invention includes illegal drug or drugs；The drugs include but not limited to methamphetamine, morphine, methyl peace
Fei Taming, Ketamine etc..
The present invention also provides a kind of for alcohol and the kit of drug test in DNA sample acquisition, saliva.The reagent
Box includes above-mentioned All-in-One sampling detector, standard color comparison card, cell-preservation liquid for measuring alcohol content in saliva sample
And sampling pipe.Wherein, cell-preservation liquid and sampling pipe are for preserving the collected oral cavity inside mucous membrane from sample detecting stick
Cell and epithelial tissue.Preferably, the cell-preservation liquid is PBS buffer solution.
The present invention further provides the All-in-One sampling detectors or kit to be acquired in saliva, DNA sample, Yi Jifei
Application in the saliva sample alcohol of diagnostic purpose and drug test.
Aforementioned applications include：Saliva sample is acquired with sample detecting stick, is added in the sample pad of drug test card, at one section
Between after observe result：Detection line and nature controlling line all develop the color, and drug test result is feminine gender；Nature controlling line develops the color, and drug to be measured corresponds to
Detection line do not develop the color, the corresponding testing result of the drug be the positive；Nature controlling line does not develop the color, and indicates invalid or failure detection knot
Fruit.
The saliva sample acquired with sample detecting stick enters test section, according to the color change and mark of alcohol detection test strips
Quasi- colorimetric card is compared, to measure the alcohol content in saliva sample.
After acquiring Oral Mucosal Cells and epithelial tissue with sample detecting stick, it is placed in cell-preservation liquid and preserves, or will adopt
Sample test bar is sealed after drying.
In the specific embodiment of the present invention, the multi-functional sample detecting stick is by sheath (can omit), sponge
Head, handle, micro- tooth scraper plate, hollow collecting pipe and alcohol detection test strips composition.Spongy top is fixed on handle front end, close to sea
The micro- tooth scraper plate of the subsidiary circle in the region of continuous head.The handle being connected with spongy top is hollow structure, and mouth is put into for collecting spongy top
The saliva that chamber is adsorbed, hollow collecting pipe are connected with the alcohol detection test strips being fixed on thereafter in handle grooves.
In the specific embodiment of the present invention, being arranged side by side along the central shaft of the drug test card has two
Sense channel is spaced between two sense channels, wherein be disposed on a sense channel 1 methamphetamine detection line, 1
Morphine detection line and 1 nature controlling line are disposed with 1 Ketamine detection line and 1 nature controlling line on another sense channel.
In use, spongy top is stretched into oral cavity, saliva is sucked and flowed into hollow handle collecting pipe by spongy top, subsequent saliva
Liquid enters with hollow collecting pipe in the alcohol detection test strips being attached thereto, can according to the variation of test strips shade and
With the comparison interpretation of standard color comparison card, to measure the content of alcohol in saliva.It can be carried out with standard color comparison card in general 2 minutes
It compares, sentence read result.Alcohol detection test strips can show that the depth of color, alcohol concentration are got over according to the content of alcohol concentration
Height, color are deeper.Using the comparison interpretation with standard color comparison card, the content of alcohol in saliva can be determined, the method is semidefinite
Amount detection.
After sample collection, spongy top is inserted into the drug test card with well, slightly exerts oneself, makes the saliva of extrusion
Whether liquid stream is compared to sample pad by band corresponding with the purpose drug of detection, judge in saliva sample containing corresponding
Drug.General 3-5 minutes observation drug test results.
Picker by sample detecting stick when being put into oral cavity acquisition saliva, along band in the oral cavity (portion on the inside of cheek
Position), by collecting oral cavity epithelial tissue with the adjacent micro- tooth scraper plate of spongy top.In the way of upper and lower scraping, 15 are about scraped per side
Under, and the head of sample detecting stick is rotated while scraping, so as to which mucosa cells are speckled on spongy top everywhere.Pay attention to：It scrapes
Scraping wound location is avoided when taking.Then micro- tooth flight portion is immersed in PBS buffer solution and is gently shaken, oral epithelium group is made
It knits and falls off, complete the collection of DNA sample.It is sealed after the sample detecting stick after collection sample can also being dried, facilitates long-distance fortune
It is defeated.
The present invention has the following advantages：
(1) simple in structure, compact is not only suitable for onsite application when law enforcement, be also very suitable for personal and family by
It need to use at any time.
(2) painless acquisition sample, without any wound or invasive, user not will produce any discomfort sense.
(3) disposable, cross-infection risk is not present.
(4) detection in real time of drugs in saliva detection and alcohol and the disposable acquisition of DNA sample can be achieved.For drink
Wine crowd, while having other detection demands (such as phase of drinking such as detection alcohol metabolism gene, alcohol addiction gene, high lithemia again
Correlation gene) individual provide simply and easily All-in-One acquisition method.
(5) DNA sample acquired is suitable for genetic analysis detection and other medical tests etc..
Description of the drawings
Fig. 1 is the multi-functional sample detecting stick for saliva, DNA sample acquisition and alcohol detection in the embodiment of the present invention 1
Structural schematic diagram；Wherein, 1- spongy tops, the micro- tooth scraper plates of 2-, the hollow collecting pipes of 3-, 4- alcohol detection test strips, 5- handles.1-
3 correspond to sampling unit, and 4 correspond to test section, and 5 correspond to grip part.
Fig. 2 is the pictorial diagram of multi-functional sample detecting stick in the embodiment of the present invention 1；Wherein, A is front, and B is reverse side.
Fig. 3 is the structural schematic diagram of narcotic saliva detection card in the embodiment of the present invention 2；Wherein, 6- sample pads, 7- colloidal golds
Bonding pad, 8- nitrocellulose filters, 9- detection lines, 10- nature controlling lines, 11- absorption pads, 12- plastics lining boards.
Fig. 4 is the vertical view of narcotic saliva detection card in the embodiment of the present invention 2；Wherein, 13- narcotic salivas detection card, 14-
Sense channel, 15- wells；1. methamphetamine detection line, 2. morphine detection line, 3. nature controlling line, 4. Ketamine detection line, 5. nature controlling line.
Fig. 5 is the pictorial diagram of narcotic saliva detection card in the embodiment of the present invention 2.
Fig. 6 is alcohol and illicit drugs inspection All-in-One sampling detector in DNA sample acquisition, saliva in the embodiment of the present invention 3
Pictorial diagram.
Fig. 7 is alcohol and illicit drugs inspection All-in-One sampling detector in DNA sample acquisition, saliva in the embodiment of the present invention 3
Combination assumption diagram；Wherein, 1- spongy tops, the micro- tooth scraper plates of 2-, the hollow collecting pipes of 3-, 4- alcohol detection test strips, 5- handles, 13-
Narcotic saliva detection card, 14- sense channels, 15- wells；1. methamphetamine detection line, 2. morphine detection line, 3. nature controlling line, 4. Ketamine
Detection line, 5. nature controlling line.
Fig. 8 is thin using mucous membrane on the inside of multi-functional sample detecting stick acquisition saliva sample and oral cavity in the embodiment of the present invention 3
The schematic diagram of born of the same parents and epithelial tissue.
Fig. 9 is 3 Plays colorimetric card schematic diagram of the embodiment of the present invention, and alcohol content can reflect blood simultaneously in saliva
The content of middle alcohol；Wherein, in from left to right indicating respectively per 100ml salivas alcohol content be respectively 0mg, 20mg, 50mg,
80mg and 300mg.
Figure 10 is alcohol detection test strips after (left side) before multi-functional sample detecting stick detection in the embodiment of the present invention 3 and detection
Colour developing situation (right side).
Figure 11 is loaded signal into the illicit drugs inspection card with well after being finished for sample collection in the embodiment of the present invention 3
Figure.
Figure 12 is the schematic diagram for carrying out illicit drugs inspection in the embodiment of the present invention 3 using All-in-One sampling detector.
Figure 13 is the testing result interpretation of narcotic saliva detection card in the embodiment of the present invention 3.
Figure 14 is that micro- tooth flight portion in the embodiment of the present invention 3 by multi-functional sample detecting stick is immersed equipped with PBS bufferings
The sampling pipe of liquid acquires the schematic diagram of DNA sample.
Figure 15 is the functional schematic of All-in-One sampling detector in the embodiment of the present invention 3.
Specific implementation mode
In the description of the present invention, unless otherwise indicated, the orientation of the instructions such as term "upper", "lower" or state relation are only
For convenience of description of the present invention and simplification of the description, specific side must be had by not indicating or implying the indicated device or element
Position, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In following embodiment make sample detecting stick handle used in material be polyvinyl chloride, polystyrene, polyethylene or
Polypropylene etc. has the material of some strength and toughness.
Multi-functional sample detecting stick of the embodiment 1 for saliva, DNA sample acquisition and alcohol detection
- Fig. 2 referring to Fig.1, it is provided in this embodiment for saliva, DNA sample acquisition and the multi-functional sampling of alcohol detection
Test bar is by sheath (can omit), spongy top, handle, micro- tooth scraper plate, hollow collecting pipe and the saliva being embedded in handle grooves
A few part compositions such as liquid alcohol detection test strips.Spongy top and micro- tooth scraper plate close to, then with before the handle of hollow belt collecting pipe
End is connected, and handle is equipped with groove, and saliva alcohol detection test paper is fixed in groove.Hollow collecting pipe one end is communicated with spongy top,
The other end is communicated with saliva alcohol detection test paper, and handle rear end is in flat, is easy to hold.
2 narcotic saliva of embodiment detection card
With reference to Fig. 3-Fig. 5, narcotic saliva detection card provided in this embodiment includes sample pad, gold conjugation pad, fixation
There are the nitrocellulose filter and absorption pad of drugs antigen to be measured.The sample pad, gold conjugation pad, to be fixed with drugs to be measured anti-
Former nitrocellulose filter and absorption pad is pasted onto on plastics lining board successively.It is arranged side by side along the central shaft of nitrocellulose filter
There are two sense channels, be spaced between two sense channels, wherein being disposed with 1 methamphetamine detection on a sense channel
Line, 1 morphine detection line and 1 nature controlling line are disposed with 1 Ketamine detection line and 1 Quality Control on another sense channel
Line.
Wherein, the gold conjugation pad is fixed with the specific antibody of anti-drugs to be measured, three kinds of methamphetamine, morphine and Ketamine
The peridium concentration of antibody is respectively 0.5mg/ml, 0.75mg/ml and 0.5mg/ml, and it is polyclonal that the nature controlling line is fixed with sheep anti mouse
Antibody, peridium concentration 2.0mg/ml.
Narcotic saliva detection card is additionally provided with a lug boss, and the boss is in the top of sample pad, the center of lug boss
If there are one well, the size of well matches with fixed sponge area of bed on the sample detecting stick of embodiment 1, sponge
The saliva of head acquisition enters sample pad by well.
Narcotic saliva detection card is additionally provided with visible observation window, which is located at the detection line and nature controlling line
Top, for illicit drugs inspection result interpretation in saliva.It is registered in addition, the blank space on visible observation window can be equipped with sample message
Column.
Kit and application of the embodiment 3 for alcohol and illicit drugs inspection in DNA sample acquisition, saliva
By embodiment 1 multi-functional sample detecting stick and embodiment 2 narcotic saliva detection card form DNA sample acquire,
Alcohol and illicit drugs inspection All-in-One sampling detector (Fig. 6 and Fig. 7) in saliva.
It is provided in this embodiment for DNA sample acquisition, the kit of alcohol and illicit drugs inspection includes above-mentioned more in saliva
Unify sampling detector, for measure the standard color comparison card, cell-preservation liquid (PBS buffer solution) of alcohol content in saliva sample with
And sampling pipe.Wherein, mucous membrane is thin on the inside of collected oral cavity from sample detecting stick for preserving for cell-preservation liquid and sampling pipe
Born of the same parents and epithelial tissue.
In use, the spongy top of multi-functional sample detecting stick is stretched into oral cavity (Fig. 8), saliva is first inhaled into spongy top
In, it is subsequently introduced in the hollow collecting pipe that sponge back of head is attached thereto, saliva wine is then flowed into from the other end by collecting pipe
In smart test strip.Final alcohol enters alcohol detection test strips by hollow collecting pipe, and alcohol detection test strips can basis
The content of alcohol concentration shows the depth of color, and alcohol concentration is higher, and color is deeper.It can be with standard ratio in general 2 minutes
Colour atla is compared, sentence read result.Using the comparison interpretation with standard color comparison card (Fig. 9), containing for alcohol in saliva can be determined
It measures (Figure 10).
After sample collection, spongy top is inserted into the illicit drugs inspection card with well (Figure 11), slightly exerts oneself, makes to squeeze
The saliva gone out flows to sample pad, is compared by band corresponding with the purpose drugs of detection, judges whether contain in saliva sample
There are corresponding drugs (Figure 12).General 3-5 minutes observation testing results.It is corresponded on detection card wired at illicit drugs inspection line (T lines)
Item is feminine gender, and no lines are the positive, and no C lines (nature controlling line) show that result is invalid (Figure 13).The detection water of methamphetamine in saliva sample
It puts down as 1000ng/ml-8mg/ml.The detection level of morphine is 300ng/ml-5mg/ml in saliva sample.Ketamine in saliva sample
Detection level be 1000ng/ml-4mg/ml.
Picker by sample detecting stick when being put into oral cavity acquisition saliva, along band in the oral cavity (portion on the inside of cheek
Position), by collecting oral cavity epithelial tissue with the adjacent micro- tooth scraper plate of spongy top.In the way of upper and lower scraping, 15 are about scraped per side
Under, and the head of sample detecting stick is rotated while scraping, so as to which mucosa cells are speckled on spongy top everywhere.Pay attention to：It scrapes
Scraping wound location is avoided when taking.Then micro- tooth flight portion is immersed in PBS buffer solution (sampling pipe) and is gently shaken, mouth is made
Chamber epithelial tissue falls off, and completes the collection (Figure 14) of DNA sample.Sample detecting stick after collection sample can also be dried rear close
Envelope, facilitates long-distance transport.
The All-in-One sampling detector of the present invention is easy to use, easy to operate, it can be achieved that painless acquisition, is examined in narcotic saliva
While survey, completes DNA sample and collect, saliva alcohol content detects three indexs (Figure 15).
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be modified or is improved, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
- Alcohol and drug test All-in-One sampling detector in 1.DNA sample collections, saliva, which is characterized in that by sample detecting Stick and drug test card composition, the sample detecting stick is for saliva, DNA sample acquisition and alcohol detection；The sample detecting stick includes grip part, sampling unit and test section；Wherein, the sampling sites in the grip part one End, and be connected with the test section being arranged on the grip part；It is fixed in the sampling unit for adsorbing saliva sample Sorbing material is provided with alcohol detection test strips on the test section；The drug test card include sample pad, gold conjugation pad, the nitrocellulose filter for being fixed with drug antigenic to be measured and Absorption pad, the nitrocellulose filter for being fixed with drug antigenic to be measured are equipped with several detection lines and at least 1 nature controlling line, The sample pad, gold conjugation pad, the nitrocellulose filter for being fixed with drug antigenic to be measured and absorption pad are pasted onto modeling successively Expect on liner plate；Wherein, the gold conjugation pad is fixed with the specific antibody of anti-drug to be measured, and the nature controlling line is fixed with sheep Anti- mouse polyclonal antibody.
- 2. sampling detector according to claim 1, which is characterized in that the grip part of the sample detecting stick and sampling unit Connected one end is hollow tubular structure, by the saliva sample of fixed sorbing material acquisition in sampling unit via hollow collection Pipe enters alcohol detection test strips；One end setting of the grip part is fluted, and it is recessed that the alcohol detection test strips are fixed on this In slot；The other end of the grip part is anti-skidding design.
- 3. sampling detector according to claim 2, which is characterized in that the sorbing material is spongy top or other nontoxic Hygroscopic material is coated with nylon membrane on the spongy top, or tightens the region subsidiary one of adjacent spongy top in the sampling unit Enclose micro- tooth scraper plate.
- 4. sampling detector according to claim 2, which is characterized in that the alcohol detection test strips groove of the test section Position directly exposes or with transparent visual windows, the visual windows are corresponding with the position of alcohol detection test strips, are used for Observe the chromogenic reaction of alcohol detection test strips.
- 5. sampling detector according to claim 1, which is characterized in that the drug test card is additionally provided with a protrusion Portion, for the boss in the top of sample pad, the center of lug boss sets that there are one well, the size of well and the samplings Fixed sorbing material size matches on test bar, and the saliva acquired on sorbing material enters sample pad by well.
- 6. sampling detector according to claim 1, which is characterized in that the drug test card is additionally provided with visible observation Window, the visible observation window are located at the top of the detection line and nature controlling line, are used for drug test result interpretation.
- 7. sampling detector according to claim 1, which is characterized in that the material of sample pad is in the drug test card The material of cellulose membrane, the gold conjugation pad is glass fibre membrane；The drug includes illegal drug or drugs；The drugs include methamphetamine, morphine, meth, Ketamine.
- 8. for alcohol and the kit of drug test in DNA sample acquisition, saliva, which is characterized in that including claim 1-7 Any one of them sampling detector, for measure in saliva sample the standard color comparison card, cell-preservation liquid of alcohol content and Sampling pipe.
- 9. kit described in any one of the claim 1-7 sampling detectors or claim 8 is acquired in saliva, DNA sample, And non-diagnostic purpose saliva sample alcohol and the application in drug test.
- 10. application according to claim 9, which is characterized in that acquire saliva sample with sample detecting stick, add to drug inspection It surveys in the sample pad of card, result is observed after a period of time：Detection line and nature controlling line all develop the color, and drug test result is feminine gender；Matter Line colour developing is controlled, the corresponding detection line of drug to be measured does not develop the color, and the corresponding testing result of the drug is the positive；Nature controlling line does not develop the color, Indicate invalid or failure detection result；The saliva sample acquired with sample detecting stick enters test section, according to the color change of alcohol detection test strips and standard ratio Colour atla is compared, to measure the alcohol content in saliva sample；It after acquiring Oral Mucosal Cells and epithelial tissue with sample detecting stick, is placed in cell-preservation liquid and preserves, or sampling is examined It surveys after stick dries and is sealed.
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