CN108414096A - Wide visual field chromatography imaging method based on adaptive optics and device - Google Patents
Wide visual field chromatography imaging method based on adaptive optics and device Download PDFInfo
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- 238000003384 imaging method Methods 0.000 title claims abstract description 57
- 230000000007 visual effect Effects 0.000 title claims abstract description 48
- 230000003044 adaptive effect Effects 0.000 title claims abstract description 44
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 12
- 230000008569 process Effects 0.000 claims abstract description 7
- 230000001351 cycling effect Effects 0.000 claims abstract description 4
- 230000003252 repetitive effect Effects 0.000 claims abstract description 4
- 239000006185 dispersion Substances 0.000 claims description 86
- 230000003287 optical effect Effects 0.000 claims description 78
- 230000010287 polarization Effects 0.000 claims description 28
- 238000000354 decomposition reaction Methods 0.000 claims description 17
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- 238000003325 tomography Methods 0.000 description 6
- 230000008901 benefit Effects 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
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- 238000009738 saturating Methods 0.000 description 2
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- 238000001228 spectrum Methods 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J11/00—Measuring the characteristics of individual optical pulses or of optical pulse trains
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
- G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
- G02B26/10—Scanning systems
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
- G02B27/283—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising used for beam splitting or combining
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Abstract
The invention discloses a kind of wide visual field chromatography imaging method and device based on adaptive optics, the wide visual field chromatography imaging method include the following steps：S1：Send out the incipient beam of light of ultrashort pulse；S2：Incipient beam of light, which is decomposed to obtain, decomposes light beam；S3：Light beam directive modulator will be decomposed, the corresponding frequency component decomposed in light beam of different modulation units of modulation is different；S4：The light beam directive measuring device that will be projected from modulator, measuring device measure the wavefront information at focal plane；S5：The optimum angle value is fed back to modulator, different modulation units is made to increase its phase with different rates by the optimum angle value for extracting each frequency component；S6：Process of the repetitive cycling from S4 to S5, until measuring device measures the wavefront information at focal plane and reaches predetermined state.Wide visual field chromatography imaging method according to the ... of the embodiment of the present invention based on adaptive optics realizes high speed, accurately measures and compensate phase distortion.
Description
Technical field
The present invention relates to optical microphotograph field more particularly to a kind of wide visual field chromatography imaging methods based on adaptive optics
And device.
Background technology
Optical microscopy imaging technology is widely used in microstructure imaging and biodynamic signal detection.Existing optics is aobvious
In micro mirror, common wide field single photon fluorescence microscope, which has that photobleaching is serious, background fluorescence is strong, does not have chromatography ability, etc. lacks
Point；Mating plate micro-imaging is placed in vertical both direction by that will excite with detection, reduces photobleaching, real in transparent sample
Tomography is showed, but it can not be applied to the biological sample with strong scattering characteristic；Common focus point scanning imagery passes through introducing
Confocal pinhole inhibits background fluorescence in a certain range, improves penetration depth, but the machinery for being constrained to scanning device is used
Property, temporal resolution is relatively low.In addition, multi-photon Mapping system uses long-wavelength excitation, penetration depth is improved, still
Its temporal resolution cannot be satisfied the demand of biomedical research.
In order to realize that wide visual field tomography, people have developed space-time focusing technology.The principle of this method is to use dispersion
Femtosecond pulse spatially dispersion is unfolded in element, makes power dissipation, then realize and assemble at focal plane, to based on non-thread
Property optical effect realize wide visual field chromatography.Space-time focusing technology realizes wide field and excites and remain chromatography ability, when improving
Between resolution ratio, compare and all have advantage for common wide-field microscope and spot scan microscope.
But in the imaging of practical Photobiology, it is contemplated that the anisotropy and heterogeneity of biological tissue, light are being organized
Experience is reflected and scattering at random when middle transmission so that focusing quality declines, and reduces the axial resolution of space-time focusing technology
And penetration depth.Specifically, in space-time focusing technology, different wave length component will undergo different light paths and reach focal plane, so will
Undergo different tissue refractions and scattering, along with tissue refraction, scattering have for the different wave length component of pulsed light it is different
It influences, therefore energy can not be assembled completely at focal plane, and launching efficiency is caused to decline, and reduce axial resolution and penetrate depth
Degree.Therefore these distortion are compensated, is very necessary.
For this purpose, there has been proposed the space-time focusing technology based on adaptive optics, by measuring different wave length component experience
Phase distortion and pre-compensated for, improve image quality.But this method searches for simultaneously the phase of distortion using greedy method
Supplement, speed is slow, is easily trapped into local minimum, can only compensate the distortion mode of low order.But ultrashort pulse is in biology
It can be influenced with scattering by random refraction during organizational communication, finally peak strength declines at focusing surface, reduces axial
Resolution ratio and penetration depth；On the other hand, in space-time focusing technology, the path that different wave length component reaches focusing surface is different,
In view of tissue anisotropy and heterogeneity, the phase distortion of exciting light experience is more non-negligible.Existing convenience is not
The compensation of phase distortion can be met.How a kind of high speed suitable for wide visual field tomography is provided, precision measuring phase position distorts
Adaptive optical technique become current significant challenge.
Invention content
The present invention is directed at least solve one of the technical problems existing in the prior art.For this purpose, the present invention proposes a kind of base
In the wide visual field chromatography imaging method of adaptive optics, the wide visual field chromatography imaging method can realize wide visual field tomography
Phase distortion accurately measuring and compensate.
The present invention, which also aims to, proposes a kind of wide visual field laminated imaging device based on adaptive optics.
According to the wide visual field chromatography imaging method based on adaptive optics, include the following steps：
S1：The incipient beam of light of ultrashort pulse is sent out, the incipient beam of light is light beam；
S2：The incipient beam of light is decomposed to obtain and decomposes light beam, the decomposition light beam in space divide by each frequency
Amount separation；
S3：By the decomposition light beam directive modulator, the modulator has multiple modulation units, the different modulation
The corresponding frequency component decomposed in light beam of unit is different；
S4：The light beam directive measuring device that will be projected from the modulator, the measuring device measure the wave at focal plane
Preceding information；
S5：The optimum angle value that each frequency component is extracted by the wavefront information feeds back to the optimum angle value
The modulator makes the different modulation units increase its phase with different rates；
S6：Process of the repetitive cycling from S4 to S5, until the measuring device measures the wavefront information at focal plane and reaches
Predetermined state.
Wide visual field chromatography imaging method according to the ... of the embodiment of the present invention based on adaptive optics, due to adding before modulation
Dispersion step is added, phase compensation can have been carried out to the light of different wavelength according to frequency to realize modulator, realized
Wavefront modification.Further, since in the imaging method of the present invention, there are feedback mechanisms between measuring device and modulator, realize
High speed accurately measures and compensates phase distortion, to realizing accurate effective observation to deep tissues.
In some embodiments, the modulator is pure phase-adjusted modulator or the modulator is intensity, phase
The modulator that position is adjusted jointly.
In some embodiments, the modulator is transmission-type modulator or the modulator is reflective modulator.
Wide visual field laminated imaging device according to the ... of the embodiment of the present invention based on adaptive optics, including：Femto-second laser,
The femto-second laser is for emitting initial light beam；Dispersion element, the dispersion element receive the femto-second laser
The incipient beam of light launched, and the incipient beam of light is resolved into decomposition light beam by the dispersion element according to frequency component；Light tune
Device processed, the optical modulator receive the decomposition light beam that the dispersion element decomposites, and the optical modulator has multiple tune
Unit processed, by the decomposition beam modulation at modulation light beam；Detector, the detector receives the modulation light beam, described
Modulation light beam is imaged on the detector；Wherein, image-forming information is fed back the optimum angle value and fed back to by the detector
The optical modulator, the multiple modulation unit adjust phase for different frequency component according to different rates；4f systems, it is described
4f systems are arranged on from the femto-second laser to the light path the detector.
Wide visual field laminated imaging device according to the ... of the embodiment of the present invention based on adaptive optics, due to adding before modulation
Dispersion step is added, phase compensation can have been carried out to the light of different wavelength according to frequency to realize optical modulator, realized
Wavefront modification.Further, since between the imaging device detector and modulator of the present invention, there are feedback mechanisms, realize height
Speed accurately measures and compensates phase distortion, to realize to being precisely effectively imaged.
In some optional embodiments, the 4f systems include the first lens and the second lens, first lens and
Second lens are convex lens, and first lens and the second lens are sequentially arranged at the member from the femto-second laser to the dispersion
In light path between part.
In some optional embodiments, the optical modulator is transmission-type modulator, and the 4f systems include that third is saturating
Mirror and the 4th lens, the third lens are located on from the dispersion element to the light path the optical modulator with to light beam
Collimated, the 4th lens be located at from the optical modulator in the light path of the detector with to light beam into line convergence.
In some optional embodiments, the wide visual field laminated imaging device based on adaptive optics further includes：
Galvanometer, the galvanometer are located on second lens to the light path of the dispersion element to change optical path direction.
Specifically, the wide visual field laminated imaging device based on adaptive optics further includes：Cylindrical lens, the column
Lens are located on the galvanometer to the light path of the dispersion element, from the galvanometer to the distance of the cylindrical lens, from the column
Lens to the dispersion element distance, it is equal with the focal length of the cylindrical lens.
Specifically, the wide visual field laminated imaging device based on adaptive optics further includes：Polarization beam apparatus, institute
State polarization beam apparatus be located at from the dispersion element to the light path of the optical modulator to change optical path direction, the light tune
Device processed is reflective modulator, and the modulation light beam that the optical modulator reflects described in the polarization beam apparatus directive by detecting
Device.
Specifically, the wide visual field laminated imaging device based on adaptive optics further includes：5th lens, it is described
5th lens are located between the dispersion element and the polarization beam apparatus；；A quarter slide, a quarter slide are set
Between the polarization beam apparatus and the optical modulator.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obviously, or practice through the invention is recognized.
Description of the drawings
The above-mentioned and/or additional aspect and advantage of the present invention will become in the description from combination following accompanying drawings to embodiment
Obviously and it is readily appreciated that, wherein：
Fig. 1 is the principle schematic of the wide visual field imaging method based on adaptive optics of the embodiment of the present invention.
Fig. 2 is the chromatic dispersion effects figure of the dispersion element of the embodiment of the present invention.
Fig. 3 is the modulation principle figure of the modulator of the embodiment of the present invention.
Fig. 4 is the structure diagram of the wide visual field imaging device based on adaptive optics of one embodiment of the invention.
Fig. 5 is the structure diagram of the wide visual field imaging device based on adaptive optics of another embodiment of the present invention.
Reference numeral：
Imaging device 1000,
Femto-second laser 100, dispersion element 110, modulator 120a, optical modulator 120b, measuring device 130a, detector
130b、
First 4f systems 140, the first lens 141, the second lens 142,
2nd 4f systems 150, the third lens 151, the 4th lens 152,
Galvanometer 160, cylindrical lens 170, polarization beam apparatus 180, a quarter slide 1900, the 5th lens 200.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, and is only used for explaining the present invention, and is not considered as limiting the invention.
In the description of the present invention, it is to be understood that, term "center", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on ... shown in the drawings or
Position relationship is merely for convenience of description of the present invention and simplification of the description, and does not indicate or imply the indicated device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.In addition, limit
There is the feature of " first ", " second " to can explicitly or implicitly include one or more this feature surely.The present invention's
In description, unless otherwise indicated, the meaning of " plurality " is two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
Can also be electrical connection to be mechanical connection；It can be directly connected, can also indirectly connected through an intermediary, Ke Yishi
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
The wide visual field tomography side according to the ... of the embodiment of the present invention based on adaptive optics is described below with reference to Fig. 1-Fig. 3
Method.
Wide visual field chromatography imaging method according to the ... of the embodiment of the present invention based on adaptive optics, includes the following steps：
S1：The incipient beam of light of ultrashort pulse is sent out, incipient beam of light is light beam；
S2：Incipient beam of light is decomposed to obtain and decomposes light beam, decomposes light beam each frequency component separation in space；
S3：Light beam directive modulator 120a will be decomposed, modulator 120a has multiple modulation units, different modulation units
Frequency component in corresponding decomposition light beam is different；
S4：The light beam directive measuring device 130a that will be projected from optical modulator 120b, measuring device 130a are measured in focal plane
The wavefront information at place；
S5：The optimum angle value is fed back to light tune by the optimum angle value that each frequency component is extracted by wavefront information
Device 120b processed, makes different modulation units increase its phase with different rates；
S6：Process of the repetitive cycling from S4 to S5, until measuring device 130a measures the wavefront information at focal plane and reaches
Predetermined state.
It is understood that as Figure 1-Figure 2, when light beam is after dispersion element 110, each frequency in space
Rate component detaches, that is to say, that when the light beam that property is polychromatic light can become different colours after dispersion element 110
Monochromatic light (shown in Fig. 2).Since modulator 120a has multiple modulation units, after the dispersion interaction of dispersion element 110,
Light beam can be irradiated at multiple surfaces of modulator 120a, hereby it is achieved that different modulation units corresponds to different frequency point
Amount.When the phase of modulator 120a variation different frequency components, the wavefront information that measuring device 130a is measured at focal plane can be sent out
Changing.Specific phase adjusted can make the fluorescence signal intensity at focal plane reach maximum value.To obtain this maximum value, pass through tune
The phase for saving modulator 120a extracts the optimum angle of optical modulator 120b from the wavefront signals changed over time measured
Value, and it is assigned to optical modulator 120b, with this so that the fluorescence signal intensity at focal plane reaches maximum.The link through iteration for several times after
Reach convergence, you can complete compensation.
That is, as shown in Fig. 2, the wide visual field chromatography imaging method based on adaptive optics of the embodiment of the present invention,
Light beam after 110 dispersion of dispersion element is compensated using light modulator modulates device 120a.The difference of light beam after dispersion
The different modulating unit of wavelength component corresponding modulating device 120a.The disparate modules of modulator 120a carry out phase to the light of different frequency
Position modulation.As shown in figure 3, when these modulation units increase phase with different rates, excites and produced by nonlinear effect on focal plane
Raw signal strength information also changes therewith, becomes the function of time.Different performance ginseng can be chosen by these strength informations
Number (such as all value becomes overall strength or overall strength plus Grad with the flatness etc. in constrained objective face to adduction), forms
Different one-dimensional time domain cost functions.Fourier transformation is carried out to one-dimensional time-domain function, due to each unit of modulator 120a
It is modulated with different orthogonal frequency, therefore the frequency spectrum after Fourier transformation is discrete.The phase of specific frequency point is extracted, and
It is assigned to corresponding modulation unit after the phase is taken negative value, is achieved that the process of a phase compensation.By iteration several times
Reach convergence later, that is, completes wavefront distortion precompensation.Meanwhile using the intensity of ultrashort pulse different frequency component at Gauss point
The relationship of cloth will be merged positioned at the modulation unit of low-intensity frequency component position, to shorten the phase-modulation time
Meanwhile ensureing that modulation accuracy is unaffected.
Wide visual field chromatography imaging method according to the ... of the embodiment of the present invention based on adaptive optics, due to adding before modulation
Add dispersion step, phase compensation can have been carried out to the light of different wavelength according to frequency to realize modulator 120a, it is real
Wavefront modification is showed.Further, since in the imaging method of the present invention, there are anti-between measuring device 130a and modulator 120a
Infeed mechanism realizes high speed, accurately measures and compensate phase distortion, to realize accurate effective observation to deep tissues.
In some embodiments, modulator 120a is pure phase-adjusted modulator or modulator 120a is intensity, phase
The modulator that position is adjusted jointly.It is understood that in actual application, the polarised light scattered by dispersion element 110
It may need phase or phase, intensity while modulate.Therefore, user can select the class of modulator 120a according to actual conditions
Type.
In some embodiments, it is reflective modulator that modulator 120a, which is transmission-type device modulator,.It should be noted that
According to the optical path direction during practice, user can voluntarily select the pattern of modulator 120a, improve this hair as a result,
The application range of the wide visual field chromatography imaging method based on adaptive optics of bright embodiment.
The wide visual field tomography dress according to the ... of the embodiment of the present invention based on adaptive optics is described below with reference to Fig. 4-Fig. 5
Set 1000.
As shown in fig. 4-5, the wide visual field laminated imaging device according to the ... of the embodiment of the present invention based on adaptive optics
1000 include femto-second laser 100, dispersion element 110, optical modulator 120b, detector 130b and 4f system.Femto-second laser
100 for emitting initial light beam, and dispersion element 110 receives the incipient beam of light that femto-second laser 100 is launched, and dispersion
Incipient beam of light is resolved into decomposition light beam by element 110 according to frequency component.Optical modulator 120b receives dispersion element 110 and decomposites
Decomposition light beam, optical modulator 120b have multiple modulation units, will decompose beam modulation at modulate light beam, detector 130b
Modulation light beam is received, modulation light beam is imaged on detector 130b.Image-forming information is fed back the optimum angle by detector 130b
Value feeds back to optical modulator 120b, and multiple modulation units adjust phase, 4f systems for different frequency component according to different rates
It is arranged on from femto-second laser 100 to the light path detector 130b.
It is understood that the wide visual field laminated imaging device 1000 based on adaptive optics of the embodiment of the present invention, right
Light beam after 110 dispersion of dispersion element is compensated using optical modulator 120b.The different wave length component of light beam after dispersion
The different modulating unit of corresponding optical modulator 120b.The disparate modules of optical modulator 120b carry out phase tune to the light of different frequency
System.When these modulation units increase phase with different rates, excites and believed by the signal strength that nonlinear effect generates on focal plane
Breath also changes therewith, becomes the function of time.By these strength informations can choose different performance parameter (such as adduction all
Value becomes overall strength or overall strength plus Grad with the flatness etc. in constrained objective face), form different one-dimensional time domain generations
Valence function.Fourier transformation is carried out to one-dimensional time-domain function, since each unit of light optical modulator 120b is with different orthogonal frequency
Rate is modulated, therefore the frequency spectrum after Fourier transformation is discrete.The phase of specific frequency point is extracted, and the phase is taken negative
It is assigned to corresponding modulation unit after value, is achieved that the process of a phase compensation.Reach convergence after iteration several times,
Complete wavefront distortion precompensation.Meanwhile using the intensity of ultrashort pulse different frequency component at the relationship of Gaussian Profile, by position
It is merged in the modulation unit of low-intensity frequency component position, to while shortening the phase-modulation time, ensure modulation
Precision is unaffected.
Wide visual field laminated imaging device 1000 according to the ... of the embodiment of the present invention based on adaptive optics, due in modulation
Before be added to dispersion step, phase benefit can be carried out to the light of different wavelength according to frequency to realize optical modulator 120b
It repays, realizes wavefront modification.Further, since being deposited between the imaging device 1000 detector 130b and optical modulator 120b of the present invention
There is feedback mechanism, is realizing high speed, accurately measures and compensate phase distortion, to realize to being precisely effectively imaged.
In some optional embodiments, as shown in fig. 4-5,4f systems include the first lens 141 and the second lens
142, the first lens 141 and the second lens 142 are convex lens, and the first lens 141 and the second lens 142 are sequentially arranged at from femtosecond
On laser 100 to the light path between dispersion element 110.It is understood that during practice, femto-second laser
The distance between 100 and dispersion element 110 can a good distance offs or close.Therefore, in femto-second laser 100 to dispersion member
The first lens 141 and the second lens 142 are arranged in light path between part 110 can ensure dispersion element 110 to a certain extent
For the chromatic dispersion effects for the polarised light that femto-second laser 100 is sent out.Certainly, what needs to be explained here is that, in practice process
In, the imaging device 1000 of the embodiment of the present invention may include multiple 4f systems, that is to say, that arrive dispersion in femto-second laser 100
Light path between element 110 is equipped with multiple first lens 141 and multiple second lens 142.
In some optional embodiments, as shown in fig. 4-5, optical modulator 120b is transmission-type light modulator 120b,
4f systems include the third lens 151 and the 4th lens 152, and the third lens 151 are located at from dispersion element 110 to optical modulator 120b
Between light path on to be collimated to light beam, the 4th lens 152 are located at the light path from optical modulator 120b to detector 130b
On with to light beam into line convergence.It is understood that the third lens 151 can ensure it is accurate by the polarised light of dispersion
Modulation unit on directive optical modulator 120b, and the 4th lens 152 can then ensure the monochrome of the different frequency through ovennodulation
Directive detector 130b after light convergence, it is therefore prevented that scattering phenomenon occurs after modulation unit for light, causes on detector 130b
Unsharp phenomenon is imaged to occur.What needs to be explained here is that according to the specifically used environment of imaging device 1000, the third lens
151 and the 4th the usage quantities of lens 152 can arbitrarily be arranged.Certainly, can also only have the third lens in some embodiments
151 either one in the 4th lens 152 or both without the third lens 151 or without the 4th lens 152.
It should be added that the third lens 151 act as collimated light beam, the 4th lens 152 act as assembling
Light beam, as long as respective function can be completed, the third lens 151 and the 4th lens 152 can be any type of lens, herein
Limitation is not made to the concrete type of the third lens 151 and the 4th lens 152.
For convenience of description, the 4f systems that the first lens 141 and the second lens 142 form hereinafter are known as the first 4f systems
140, the third lens 151 and the 4th lens 152 composition 4f systems be known as the 2nd 4f systems 150.
In some optional embodiments, as shown in figure 5, the wide visual field laminated imaging device 1000 based on adaptive optics
Further include galvanometer 160, galvanometer 160 is located on the second lens 142 to the light path of dispersion element 110 to change optical path direction.Due to
Galvanometer 160 can change optical path direction, it is possible thereby to which so that the polarised light generation deflecting that femto-second laser 100 is sent out, improves
The scope of application of imaging device 1000.Certainly, it should be noted that in an embodiment of the present invention, the number and tool of galvanometer 160
The direction of body can be arranged according to actual needs.
Specifically, as shown in figure 5, the wide visual field laminated imaging device 1000 based on adaptive optics further includes cylindrical lens
170, cylindrical lens 170 are located on galvanometer 160 to the light path of dispersion element 110, from galvanometer 160 to the distance of cylindrical lens 170, from column
Lens 170 arrive the distance of dispersion element 110, equal with the focal length of cylindrical lens 170.There is cylindrical lens 170 certain convergence to make
With the light beam that setting cylindrical lens 170 can reflect galvanometer 160 in the light path of galvanometer 160 to dispersion element 110 has
Certain converging action, to ensure that the light beam that galvanometer 160 reflects being capable of preferably directive dispersion element 110.
Specifically, as shown in figure 5, the wide visual field laminated imaging device 1000 based on adaptive optics includes polarization beam apparatus
180, polarization beam apparatus 180 be located at from dispersion element 110 to the light path of optical modulator 120b to change optical path direction, light tune
Device 120b processed is reflection optical modulator 120b, and the modulation light beam that optical modulator 120b is reflected is penetrated by polarization beam apparatus 180
To detector 130b.It should be noted that the use occasion smaller in certain spaces, in order to save space, by optical modulator
120b is set as reflection optical modulator 120b, that is to say, that the light receiver direction of optical modulator 120b and direction of the launch phase
Instead, then the phenomenon in the light that optical modulator 120b is sent out in order to prevent and retroeflection to dispersion element 110 occurs, in dispersion member
Polarization beam apparatus 180 is set between part 110 and the light path of optical modulator 120b, realizes the light sent out from dispersion element 110
Can only directive optical modulator 120b, the light sent out from optical modulator 120b can only directive detector 130b.
Specifically, as shown in figure 5, the wide visual field laminated imaging device 1000 based on adaptive optics further includes the 5th lens
200 and a quarter slide 190, the 5th lens 200 be located between polarization beam apparatus 180 and dispersion element 110, a quarter glass
Piece 190 is located between polarization beam apparatus 180 and optical modulator 120b.It is understood that the 5th lens 200 can play collimation
Effect, ensure that the directive optical modulator 120b that the light beam by polarization beam apparatus 180 can be collimated more.Simultaneously, by
Be located between polarization beam apparatus 180 and light modulator in a quarter slide 190, then light beam successively pass through dispersion element 110, partially
It shakes and reaches optical modulator 120b after beam splitter 180, a quarter slide 190, later using one of four parts slides, polarization beam splittings
Device 180 reaches detector 130b, and according to optical principle, for polarised light after two a quarter slides 190, polarization direction can be with
Originally vertical, that is to say, that the light beam that the light beam through 110 dispersion of dispersion element passes through a quarter slide 190 with second
Biased direction is orthogonal, and being achieved can only directive after polarization beam apparatus 180 from the light that dispersion element 110 is sent out
Optical modulator 120b, the light sent out from optical modulator 120b can only after a quarter slide 190 and polarization beam apparatus 180
Directive detector 130b.
Below with reference to Fig. 4-Fig. 5 description two specific embodiments of the present invention the wide visual field chromatography based on adaptive optics at
As device 1000.
Embodiment 1：
The space-time that the imaging device 1000 of the present embodiment is suitable for open side excitation focuses microscopic system, as shown in figure 4, imaging
Device 1000 include femto-second laser 100, dispersion element 110, optical modulator 120b, detector 130b, the first 4f systems 140 and
2nd 4f systems 150.Femto-second laser 100 receives femto-second laser for emitting initial light beam, dispersion element 110
100 incipient beam of light launched, and incipient beam of light is resolved into decomposition light beam by dispersion element 110 according to frequency component.Light modulation
Device 120b receives the decomposition light beam that dispersion element 110 decomposites, and optical modulator 120b has multiple modulation units, will decompose light
Beam is modulated into modulation light beam, and optical modulator 120b is formed as transmission-type light modulator 120b.Detector 130b receives modulation light beam,
Modulation light beam is imaged on detector 130b.Image-forming information is fed back the optimum angle value and feeds back to light modulation by detector 130b
Device 120b, multiple modulation units adjust phase for different frequency component according to different rates, and the first 4f systems 140 include first
Lens 141 and the second lens 142, the first lens 141 and the second lens 142 are convex lens, the first lens 141 and the second lens
142 are sequentially arranged on from femto-second laser 100 to the light path dispersion element 110.2nd 4f systems 150 include 4f system packets
The third lens 151 and the 4th lens 152 are included, the third lens 151 are located at from dispersion element 110 to the light optical modulator 120b
Road to be collimated to light beam, the 4th lens 152 be located at from optical modulator 120b in the light path of detector 130b with to light
Beam is into line convergence.
In the present embodiment, the light that femto-second laser 100 is sent out is saturating using third after 110 dispersion of dispersion element
After mirror 151 collimates, it is modulated on focal plane by optical modulator 120b, modulated light is assembled using the 4th lens 152,
Image planes are formed at detector 130b.Light distribution in detector 130b record image planes, feeds back to light after forming cost function
Modulator 120b is modulated.
Embodiment 2：
The space-time that the imaging device 1000 of the present embodiment is suitable for line scanning excitation focuses microscopic system, as shown in figure 5, imaging
Device 1000 include femto-second laser 100, dispersion element 110, optical modulator 120b, detector 130b, the first 4f systems 140,
2nd 4f systems 150, galvanometer 160, cylindrical lens 170, polarization beam apparatus 180, a quarter slide 190 and the 5th lens 200.
Femto-second laser 100 receives femto-second laser 100 and emits for emitting initial light beam, dispersion element 110
The incipient beam of light gone out, and incipient beam of light is resolved into decomposition light beam by dispersion element 110 according to frequency component.Optical modulator 120b connects
Receive the decomposition light beam that decomposites of dispersion element 110, optical modulator 120b has multiple modulation units, will decompose beam modulation at
Light beam is modulated, optical modulator 120b is formed as reflection optical modulator 120b.Detector 130b receives modulation light beam, modulates light beam
It is imaged on detector 130b.Image-forming information is fed back the optimum angle value and feeds back to optical modulator 120b by detector 130b,
Multiple modulation units adjust phase for different frequency component according to different rates.
First 4f systems 140 include the first lens 141 and the second lens 142, and the first lens 141 and the second lens 142 are
For convex lens, the first lens 141 and the second lens 142 are sequentially arranged at from femto-second laser 100 to the light dispersion element 110
On the road.Cylindrical lens 170 are located on galvanometer 160 to the light path of dispersion element 110, from galvanometer 160 to the distance of cylindrical lens 170, from
Cylindrical lens 170 arrive the distance of dispersion element 110, equal with the focal length of cylindrical lens 170.Polarization beam apparatus 180 is located at from dispersion
Element 110 between the light path of optical modulator 120b to change optical path direction.2nd 4f systems 150 include third including 4f systems
Lens 151 and the 4th lens 152, the third lens 151 are located on from dispersion element 110 to the light path polarization beam apparatus 180
To be collimated to light beam, the 4th lens 152 be located at from optical modulator 120b to light path on to light beam into line convergence.5th
Lens 200 are located between polarization beam apparatus 180 and dispersion element 110, and a quarter slide 190 is located at 180 He of polarization beam apparatus
Between optical modulator 120b.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " illustrative examples ",
The description of " example ", " specific example " or " some examples " etc. means specific features described in conjunction with this embodiment or example, knot
Structure, material or feature are included at least one embodiment or example of the invention.In the present specification, to above-mentioned term
Schematic representation may not refer to the same embodiment or example.Moreover, specific features, structure, material or the spy of description
Point can be combined in any suitable manner in any one or more of the embodiments or examples.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of being detached from the principle of the present invention and objective a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The range of invention is limited by claim and its equivalent.
Claims (10)
1. a kind of wide visual field chromatography imaging method based on adaptive optics, which is characterized in that include the following steps：
S1：The incipient beam of light of ultrashort pulse is sent out, the incipient beam of light is light beam；
S2：The incipient beam of light is decomposed to obtain and decomposes light beam, the decomposition light beam in space divide by each frequency component
From；
S3：By the decomposition light beam directive modulator, the modulator has multiple modulation units, the different modulation units
The corresponding frequency component decomposed in light beam is different；
S4：The light beam directive measuring device that will be projected from the modulator, the measuring device measure the letter of the wavefront at focal plane
Breath；
S5：The optimum angle value that each frequency component is extracted by the wavefront information feeds back to the optimum angle value described
Modulator makes the different modulation units increase its phase with different rates；
S6：Process of the repetitive cycling from S4 to S5, until the measuring device measures the wavefront information at focal plane and reaches predetermined
State.
2. the wide visual field chromatography imaging method according to claim 1 based on adaptive optics, which is characterized in that the tune
Device processed is that pure phase-adjusted modulator or the modulator are the modulator that intensity, phase are adjusted jointly.
3. the wide visual field chromatography imaging method according to claim 1 based on adaptive optics, which is characterized in that the tune
Device processed is transmission-type modulator or the modulator is reflective modulator.
4. a kind of wide visual field laminated imaging device based on adaptive optics, which is characterized in that including：
Femto-second laser, the femto-second laser is for emitting initial light beam；
Dispersion element, the dispersion element receives the incipient beam of light that the femto-second laser is launched, and the dispersion element will
The incipient beam of light resolves into decomposition light beam according to frequency component；
Optical modulator, the optical modulator receive the decomposition light beam that the dispersion element decomposites, the light modulation utensil
There are multiple modulation units, by the decomposition beam modulation at modulation light beam；
Detector, the detector receive the modulation light beam, and the modulation light beam is imaged on the detector；Wherein, institute
It states detector image-forming information is fed back to the optimum angle value gone out and feed back to the optical modulator, the multiple modulation unit is not for
Same frequency component adjusts phase according to different rates；
4f systems, the 4f systems are arranged on from the femto-second laser to the light path the detector.
5. the wide visual field laminated imaging device according to claim 4 based on adaptive optics, which is characterized in that the 4f
System includes the first lens and the second lens, and first lens and the second lens are convex lens, first lens and the
Two lens are sequentially arranged on from the femto-second laser to the light path the dispersion element.
6. the wide visual field laminated imaging device according to claim 4 based on adaptive optics, which is characterized in that the light
Modulator is transmission-type modulator, and the 4f systems include the third lens and the 4th lens, and the third lens are located at from described
To be collimated to light beam on dispersion element to the light path between the optical modulator, the 4th lens are located at from the light tune
On device processed to the light path of the detector with to light beam into line convergence.
7. the wide visual field laminated imaging device according to claim 5 based on adaptive optics, which is characterized in that also wrap
It includes：Galvanometer, the galvanometer are located on second lens to the light path of the dispersion element to change optical path direction.
8. the wide visual field laminated imaging device according to claim 7 based on adaptive optics, which is characterized in that also wrap
It includes：Cylindrical lens, the cylindrical lens are located on the galvanometer to the light path of the dispersion element, from the galvanometer to the cylindrical lens
Distance, from the cylindrical lens to the distance of the dispersion element, it is equal with the focal length of the cylindrical lens.
9. the wide visual field laminated imaging device according to claim 4 based on adaptive optics, which is characterized in that also wrap
It includes：Polarization beam apparatus, the polarization beam apparatus be located at from the dispersion element to the light path of the optical modulator with change
Optical path direction, the optical modulator are reflective modulator, and the modulation light beam that the optical modulator reflects passes through the polarization
Detector described in beam splitter directive.
10. the wide visual field laminated imaging device according to claim 9 based on adaptive optics, which is characterized in that also wrap
It includes：5th lens, the 5th lens are located between the dispersion element and the polarization beam apparatus；
A quarter slide, a quarter slide are located between the polarization beam apparatus and the optical modulator.
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