CN108403474A - A kind of preparation of soy molasses biology cellulose moisture saver mask - Google Patents
A kind of preparation of soy molasses biology cellulose moisture saver mask Download PDFInfo
- Publication number
- CN108403474A CN108403474A CN201810369455.9A CN201810369455A CN108403474A CN 108403474 A CN108403474 A CN 108403474A CN 201810369455 A CN201810369455 A CN 201810369455A CN 108403474 A CN108403474 A CN 108403474A
- Authority
- CN
- China
- Prior art keywords
- soy molasses
- biology cellulose
- facial mask
- culture medium
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/731—Cellulose; Quaternized cellulose derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8141—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
- A61K8/8147—Homopolymers or copolymers of acids; Metal or ammonium salts thereof, e.g. crotonic acid, (meth)acrylic acid; Compositions of derivatives of such polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/89—Polysiloxanes
- A61K8/891—Polysiloxanes saturated, e.g. dimethicone, phenyl trimethicone, C24-C28 methicone or stearyl dimethicone
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/008—Preparations for oily skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
Abstract
The present invention relates to a kind of preparations of soy molasses biology cellulose moisture saver mask, it is to be inoculated into soy molasses culture medium by the Xing Shi bacillus of the coltfoal between two parties CGMCC12562 (Komagataeibacter intermedius CGMCC12562) after seed culture medium expands, biology cellulose basement membrane is generated, then coordinates (water phase with Essence：Deionized water 72% 90%, propylene glycol 2% 6%, EDTA 2Na 0.03% 0.07%, PVP 0.02% 0.06%, carbomer 0.1% 0.5%；Oil phase：0.5%) glycerine 4% 8%, dimethyl silicone polymer 3% 7%, vitamin E 1% 5%, methyl hydroxybenzoate 0.1% prepare biology cellulose facial mask, and the pH to facial mask, water retention, moisturizing rate, heat-resisting and cold-resistant stability are measured.The present invention uses soy molasses for fermentation substrate, prepares biology cellulose facial mask, possesses higher moisture holding capacity, anti-pull ability, and cooperation is without any side effects with the Essence that glycerine, propylene glycol and carbomer etc. are prepared by raw material；Soy molasses can reduce the cost of production facial mask as fermentation substrate, meanwhile, biology cellulose facial mask can degrade, and will not cause environmental pollution.
Description
Technical field
The invention belongs to soy molasses utilization and facial mask fields, and in particular to a kind of soy molasses biology cellulose moisturizing face
The preparation of film.
Background technology
Soy molasses are as the by-product generated during alcohol method production soy protein concentrate, needed for microorganism growth
The nutrition wanted and water-soluble mineral element, are good natural microbial leavenings, as having a extensive future for fermentation substrate,
Multiple types fermented product can be produced, soy molasses are cheap, and production cost can be substantially reduced as fermentation substrate.
Facial mask is to be temporarily isolating extraneous air and pollution using the of short duration time for being covered in face, improves skin temperature,
The pore of skin is expanded, and promotes sweat gland secretion and metabolism, the oxygen content of skin is made to increase, and it is thin to be conducive to skin exclusion epidermis
The oil substances of the product and accumulation of born of the same parents' metabolism, the moisture content in facial mask penetrate into the cuticula of skin epidermis, and skin becomes
Softness, skin light are flexible.With the development of the society, expanding economy, people increasingly pay attention to the maintenance for face, beautiful
Hold skincare product almost at product indispensable in people's daily life, facial mask also becomes ever more important.
When using biology cellulose facial mask, skin temperature increases, and secretory activity is vigorous, pore expands, and when stripping, is conducive to
Dirt in skin is taken away together, while the active ingredient in facial mask can also penetrate into skin, play the role of skin care.Biology
The Graded Density structure of cellulose facial mask can effectively adsorb the excess oil that skin is up to, the grease in pore be exported, simultaneously
Achieve the purpose that moisturizing and purification.And not yet it is seen in report by the preparation of the bacterial cellulose facial mask of substrate of soy molasses.
Invention content
The present invention provides a kind of preparations of soy molasses biology cellulose moisture saver mask.The facial mask is relative to generally with nothing
Woven fabric be matrix molding class facial mask the difference is that, the present invention uses soy molasses for fermentation substrate, prepares biology cellulose
Facial mask possesses higher moisture holding capacity, anti-pull ability, and cooperation is with the essence that glycerine, propylene glycol and carbomer etc. are prepared by raw material
Magnificent liquid, without any side effects, short time use can win initial success, and be used for a long time, then can generate good whitening, moisturizing, control
Oil is repaired, convergence pore, the skincare effects such as fine and smooth skin；Production face can be reduced using soy molasses as fermentation substrate
The cost of film, meanwhile, soy molasses biology cellulose facial mask can degrade, and will not cause environmental pollution.
The technical solution adopted by the present invention：
A kind of preparation of soy molasses biology cellulose moisture saver mask is by through seed culture medium (glucose 2%, ferment
Mother's leaching powder 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, citric acid 0.115%, remaining water polishing, adjust pH
=6) the Xing Shi bacillus of the coltfoal between two parties CGMCC12562 (Komagataeibacter intermedius CGMCC12562) after expanding
Be inoculated into soy molasses culture medium (soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%,
Malic acid 0.4% adjusts PH=6), biology cellulose basement membrane is generated, then coordinate (water phase with Essence：Deionized water 72%-
90%, propylene glycol 2%-6%, EDTA-2Na 0.03%-0.07%, PVP0.02%-0.06%, carbomer 0.1%-0.5%；
Oil phase：Glycerine 4%-8%, dimethyl silicone polymer 3%-7%, vitamin E 1%-5%, methyl hydroxybenzoate 0.1%-0.5%)
Biology cellulose facial mask is prepared, and the pH to facial mask, water retention, moisturizing rate, heat-resisting and cold-resistant stability are measured.
A kind of preparation of soy molasses biology cellulose moisture saver mask, step are：
(1) prepared by soy molasses biological fiber basement membrane
1) preparation of culture medium
Seed culture medium：Glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, lemon
Acid 0.115%, it is remaining to use water polishing, adjust pH=6；
Molasses culture medium：Soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, apple
Tartaric acid 0.4% adjusts PH=6；
Culture medium ultraviolet irradiation and is cooled to room temperature after high pressure steam sterilization；
2) it is inoculated with stationary culture
It will between two parties coltfoal Xing Shi bacillus CGMCC12562 (Komagataeibacter intermedius CGMCC12562) points
Be not inoculated in seed culture medium and spread cultivation, 22h is cultivated in the shaking table culture case (30 DEG C, 160r), after be inoculated in molasses culture medium
In, it is drawn off when bacteria cellulose film thickness reaches 3~5cm to get to soy molasses biology cellulose basement membrane；
3) biology cellulose basement membrane post-processes
Cultured biological cellulose membrane is boiled into 2h in 80 DEG C of water-baths with 0.1% sodium hydroxide, is washed away on face mask substrate material
Loose colour, modulation pH are neutrality.The dry 4h in baking oven, it is soy molasses biology cellulose film to take out；
(2) prepared by Essence
Water phase (appropriate amount of deionized water, propylene glycol, EDTA-2Na, PVP) is added to magnetic stirring apparatus, starts to stir, then
Appropriate carbomer is added, makes respectively to expect that body is uniformly dispersed in heating process, for use；Qs glycerin, dimethyl silicone polymer are added
Oil phase pot, makes it completely dissolved, for use；It vacuumizes and oil phase pot material is pumped into emulsification pot, vitamin E is added, homogeneous makes respectively
Material dispersion is uniformly dissolved, and adds methyl hydroxybenzoate, after stirring evenly, filtering and discharging；
(3) prepared by facial mask
By treated, biology cellulose is thin film dipped in the Essence of preparation, then takes out high pressure sterilization and packs low
Temperature is sealed up for safekeeping, as soy molasses biology cellulose facial mask.
(4) facial mask performance evaluation
1) pH is measured
Using dilution method：Film sample 1g is taken, distilled water 10g is added, is heated to 40 DEG C, is stirred until homogeneous, be cooled to room
Temperature is measured with pH meter；
2) measurement of water-retaining property
Weigh the sample m of a certain amount of moisture content0It is placed in drier (placing the adsorbents such as silica gel) and dries to quality perseverance
It is fixed, weigh constant weight quality m after sample dryingn, the water retention of sample is calculated, calculation formula is：
Water retention/%=mn/m0× 100,
3) measurement of moisturizing rate
The moisture value x that any cosmetics hand is not smeared in cleaning later is measured using moisture of skin tester0, will configure
Facial mask be applied to the identical position of hand, removed after applying 10min, timing (0.5,1,2,4,6 and 10h) measures hand water
Score value xn, moisturizing rate is calculated, calculation formula is：
Moisturizing rate/%=(xn-x0)/x0×100
4) stability test
Heat resistant test is to be placed in 40 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change；Cold-resistant examination
It tests to be placed in -5 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change.
Preferably, the percentage in the Essence shared by glycerine, propylene glycol and carbomer is respectively 5%, 4% and
0.2%；
It is as preferred embodiment of the invention：A kind of preparation of soy molasses biology cellulose moisture saver mask, is by passing through
Seed culture medium (glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, citric acid
0.115%, it is remaining use water polishing, adjust pH=6) amplification after the Xing Shi bacillus of coltfoal between two parties CGMCC12562
(Komagataeibacter intermedius CGMCC12562) is inoculated into soy molasses culture medium (soy molasses solid content
Content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, malic acid 0.4%, PH=6 is adjusted), generate biological fiber
Plain basement membrane, then coordinate (water phase with Essence：Deionized water 83.5%, propylene glycol 4%, EDTA-2Na0.05%, PVP
0.05%, carbomer 0.2%；Oil phase：Glycerine 5%, dimethyl silicone polymer 5%, vitamin E 2%, methyl hydroxybenzoate
0.2%) biology cellulose facial mask is prepared.
Compared to existing technology, beneficial effects of the present invention are：The present invention uses soy molasses to be made for fermentation substrate
Biology cellulose facial mask, raw material is extensive, green non-pollution, can reduce the production cost of facial mask, and biology cellulose obtained
Facial mask possesses higher moisture holding capacity, anti-pull ability, and Essence is not easy to evapotranspire, and extends the effect that active ingredient enters skin
Time, while soy molasses biology cellulose facial mask can degrade, and environmental pollution, processing method of the present invention will not be caused simply easy
In operation.
Description of the drawings
Fig. 1 is process flow chart prepared by soy molasses biology cellulose basement membrane
Fig. 2 is the preparation technology flow chart of Essence
Fig. 3 is the process flow chart that soy molasses biology cellulose basement membrane and Essence prepare facial mask
Fig. 4 is the schematic diagram of soy molasses biology cellulose facial mask
Fig. 5 is section explosion views of the Fig. 4 along horizontal center line
Specific implementation mode：
The present invention is explained further below in conjunction with specific embodiment, but embodiment does not do any type of limit to the present invention
It is fixed.
Percentage involved in following embodiment is weight percentage
A kind of preparation of 1 soy molasses biology cellulose moisture saver mask of embodiment, step are：
(1) prepared by soy molasses biological fiber basement membrane
1) preparation of culture medium
Seed culture medium：Glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, lemon
Acid 0.115%, it is remaining to use water polishing, adjust pH=6；
Molasses culture medium：Soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, apple
Tartaric acid 0.4% adjusts PH=6；
Culture medium ultraviolet irradiation and is cooled to room temperature after high pressure steam sterilization；
2) it is inoculated with stationary culture
It will between two parties coltfoal Xing Shi bacillus CGMCC12562 (Komagataeibacter intermedius CGMCC12562) points
Be not inoculated in seed culture medium and spread cultivation, 22h is cultivated in the shaking table culture case (30 DEG C, 160r), after be inoculated in molasses culture medium
In, it is drawn off when bacteria cellulose film thickness reaches 3~5cm to get to soy molasses biology cellulose basement membrane；
3) biology cellulose basement membrane post-processes
Cultured biological cellulose membrane is boiled into 2h in 80 DEG C of water-baths with 0.1% sodium hydroxide, is washed away on face mask substrate material
Loose colour, modulation pH is neutrality, and the dry 4h in baking oven, it is soy molasses biology cellulose film to take out；
(2) prepared by Essence
Water phase (deionized water 82.6%, propylene glycol 5%, EDTA-2Na 0.05%, PVP0.05%) is added to magnetic force
Blender starts to stir, and adds carbomer 0.1%, makes respectively to expect that body is uniformly dispersed in heating process, for use；By glycerine 5%,
Oil phase pot is added in dimethyl silicone polymer 5%, makes it completely dissolved, for use；It vacuumizes and oil phase pot material is pumped into emulsification pot,
Vitamin E 2% is added, homogeneous makes each material dispersion be uniformly dissolved, adds methyl hydroxybenzoate 0.2%, after stirring evenly, mistake
Filter out material；
(3) prepared by facial mask
By treated, biology cellulose is thin film dipped in the Essence of preparation, then takes out high pressure sterilization and packs low
Temperature is sealed up for safekeeping, as soy molasses biology cellulose facial mask.
(4) facial mask performance evaluation
1) pH is measured
Using dilution method：Film sample 1g is taken, distilled water 10g is added, is heated to 40 DEG C, is stirred until homogeneous, be cooled to room
Temperature is measured with pH meter；
2) measurement of water-retaining property
Weigh the sample m of a certain amount of moisture content0It is placed in drier (placing the adsorbents such as silica gel) and dries to quality perseverance
It is fixed, weigh constant weight quality m after sample dryingn, the water retention of sample is calculated, calculation formula is：
Water retention/%=mn/m0× 100,
3) measurement of moisturizing rate
The moisture value x that any cosmetics hand is not smeared in cleaning later is measured using moisture of skin tester0, will configure
Facial mask be applied to the identical position of hand, removed after applying 10min, timing (0.5,1,2,4,6 and 10h) measures hand water
Score value xn, moisturizing rate is calculated, calculation formula is：
Moisturizing rate/%=(xn-x0)/x0×100
4) stability test
Heat resistant test is to be placed in 40 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change；Cold-resistant examination
It tests to be placed in -5 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change.
A kind of preparation of 2 soy molasses biology cellulose moisture saver mask of embodiment, step are：
(1) prepared by soy molasses biological fiber basement membrane
1) preparation of culture medium
Seed culture medium：Glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, lemon
Acid 0.115%, it is remaining to use water polishing, adjust pH=6；
Molasses culture medium：Soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, apple
Tartaric acid 0.4% adjusts PH=6；
Culture medium ultraviolet irradiation and is cooled to room temperature after high pressure steam sterilization；
2) it is inoculated with stationary culture
It will between two parties coltfoal Xing Shi bacillus CGMCC12562 (Komagataeibacter intermedius CGMCC12562) points
Be not inoculated in seed culture medium and spread cultivation, 22h is cultivated in the shaking table culture case (30 DEG C, 160r), after be inoculated in molasses culture medium
In, it is drawn off when bacteria cellulose film thickness reaches 3~5cm to get to soy molasses biology cellulose basement membrane；
3) biology cellulose basement membrane post-processes
Cultured biological cellulose membrane is boiled into 2h in 80 DEG C of water-baths with 0.1% sodium hydroxide, is washed away on face mask substrate material
Loose colour, modulation pH are neutrality.The dry 4h in baking oven, it is soy molasses biology cellulose film to take out；
(2) prepared by Essence
Water phase (deionized water 82.4%, propylene glycol 4%, EDTA-2Na 0.05%, PVP0.05%) is added to magnetic force
Blender starts to stir, and adds carbomer 0.3%, makes respectively to expect that body is uniformly dispersed in heating process, for use；By glycerine 6%,
Oil phase pot is added in dimethyl silicone polymer 5%, makes it completely dissolved, for use；It vacuumizes and oil phase pot material is pumped into emulsification pot,
Vitamin E 2% is added, homogeneous makes each material dispersion be uniformly dissolved, adds methyl hydroxybenzoate 0.2%, after stirring evenly, mistake
Filter out material；
(3) prepared by facial mask
By treated, biology cellulose is thin film dipped in the Essence of preparation, then takes out high pressure sterilization and packs low
Temperature is sealed up for safekeeping, as soy molasses biology cellulose facial mask.
(4) facial mask performance evaluation
1) pH is measured
Using dilution method：Film sample 1g is taken, distilled water 10g is added, is heated to 40 DEG C, is stirred until homogeneous, be cooled to room
Temperature is measured with pH meter；
2) measurement of water-retaining property
Weigh the sample m of a certain amount of moisture content0It is placed in drier (placing the adsorbents such as silica gel) and dries to quality perseverance
It is fixed, weigh constant weight quality m after sample dryingn, the water retention of sample is calculated, calculation formula is：
Water retention/%=mn/m0× 100,
3) measurement of moisturizing rate
The moisture value x that any cosmetics hand is not smeared in cleaning later is measured using moisture of skin tester0, will configure
Facial mask be applied to the identical position of hand, removed after applying 10min, timing (0.5,1,2,4,6 and 10h) measures hand water
Score value xn, moisturizing rate is calculated, calculation formula is：
Moisturizing rate/%=(xn-x0)/x0×100
4) stability test
Heat resistant test is to be placed in 40 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change；Cold-resistant examination
It tests to be placed in -5 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change.
A kind of preparation of 3 soy molasses biology cellulose moisture saver mask of embodiment, step are：
(1) prepared by soy molasses biological fiber basement membrane
1) preparation of culture medium
Seed culture medium：Glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, lemon
Acid 0.115%, it is remaining to use water polishing, adjust pH=6；
Molasses culture medium：Soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, apple
Tartaric acid 0.4% adjusts PH=6；
Culture medium ultraviolet irradiation and is cooled to room temperature after high pressure steam sterilization；
2) it is inoculated with stationary culture
It will between two parties coltfoal Xing Shi bacillus CGMCC12562 (Komagataeibacter intermedius CGMCC12562) points
Be not inoculated in seed culture medium and spread cultivation, 22h is cultivated in the shaking table culture case (30 DEG C, 160r), after be inoculated in molasses culture medium
In, it is drawn off when bacteria cellulose film thickness reaches 3~5cm to get to soy molasses biology cellulose basement membrane；
3) biology cellulose basement membrane post-processes
Cultured biological cellulose membrane is boiled into 2h in 80 DEG C of water-baths with 0.1% sodium hydroxide, is washed away on face mask substrate material
Loose colour, modulation pH are neutrality.The dry 4h in baking oven, it is soy molasses biology cellulose film to take out；
(2) prepared by Essence
Water phase (deionized water 82.5%, propylene glycol 6%, EDTA-2Na 0.05%, PVP0.05%) is added to magnetic force
Blender starts to stir, and adds carbomer 0.2%, makes respectively to expect that body is uniformly dispersed in heating process, for use；By glycerine 4%,
Oil phase pot is added in dimethyl silicone polymer 5%, makes it completely dissolved, for use；It vacuumizes and oil phase pot material is pumped into emulsification pot,
Vitamin E 2% is added, homogeneous makes each material dispersion be uniformly dissolved, adds methyl hydroxybenzoate 0.2%, after stirring evenly, mistake
Filter out material；
(3) prepared by facial mask
By treated, biology cellulose is thin film dipped in the Essence of preparation, then takes out high pressure sterilization and packs low
Temperature is sealed up for safekeeping, as soy molasses biology cellulose facial mask.
(4) facial mask performance evaluation
1) pH is measured
Using dilution method：Film sample 1g is taken, distilled water 10g is added, is heated to 40 DEG C, is stirred until homogeneous, be cooled to room
Temperature is measured with pH meter；
2) measurement of water-retaining property
Weigh the sample m of a certain amount of moisture content0It is placed in drier (placing the adsorbents such as silica gel) and dries to quality perseverance
It is fixed, weigh constant weight quality m after sample dryingn, the water retention of sample is calculated, calculation formula is：
Water retention/%=mn/m0× 100,
3) measurement of moisturizing rate
The moisture value x that any cosmetics hand is not smeared in cleaning later is measured using moisture of skin tester0, will configure
Facial mask be applied to the identical position of hand, removed after applying 10min, timing (0.5,1,2,4,6 and 10h) measures hand water
Score value xn, moisturizing rate is calculated, calculation formula is：
Moisturizing rate/%=(xn-x0)/x0×100
4) stability test
Heat resistant test is to be placed in 40 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change；Cold-resistant examination
It tests to be placed in -5 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change.
Protection scope of the present invention is not limited to aforesaid operations example, and all technologies identical or equivalent with the present invention are each fallen within
In protection scope of the present invention.
Claims (7)
1. a kind of preparation of soy molasses biology cellulose moisture saver mask, it is characterised in that；It is by through seed culture medium (grape
Sugar 2%, peptone 0.5%, disodium hydrogen phosphate 0.27%, citric acid 0.115%, remaining is mended at yeast extract 0.5% with water
Together, adjust pH=6) amplification after the Xing Shi bacillus CGMCC12562 of coltfoal between two parties (Komagataeibacter intermedius
CGMCC12562 soy molasses culture medium (soy molasses solid content 15Brix, corn protein powder 0.15%, sulphur) are inoculated into
Sour zinc 0.15%, adjusts PH=6 at malic acid 0.4%), biology cellulose basement membrane is generated, then coordinate (water phase with Essence：Go from
Sub- water 72%-90%, propylene glycol 2%-6%, EDTA-2Na 0.03%-0.07%, PVP 0.02%-0.06%, carbomer
0.1%-0.5%；Oil phase：Glycerine 4%-8%, dimethyl silicone polymer 3%-7%, vitamin E 1%-5%, methyl hydroxybenzoate
0.1%-0.5%) prepare biology cellulose facial mask, and the pH to facial mask, water retention, moisturizing rate, heat-resisting and cold-resistant stability into
Row measures.
2. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that it is
It is realized by following steps：
(1) prepared by soy molasses biological fiber basement membrane
1) preparation of culture medium
Seed culture medium：Glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, citric acid
0.115%, it is remaining to use water polishing, adjust pH=6；
Molasses culture medium：Soy molasses solid content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, malic acid
0.4%, adjust PH=6；
Culture medium ultraviolet irradiation and is cooled to room temperature after high pressure steam sterilization；
2) it is inoculated with stationary culture
It coltfoal Xing Shi bacillus CGMCC12562 (Komagataeibacter intermedius CGMCC12562) will connect respectively between two parties
Kind spread cultivation in seed culture medium, 22h cultivated in shaking table culture case (30 DEG C, 160r), after be inoculated in molasses culture medium, when
Bacteria cellulose film thickness is drawn off when reaching 3~5cm to get to soy molasses biology cellulose basement membrane；
3) biology cellulose basement membrane post-processes
Cultured biological cellulose membrane is boiled into 2h in 80 DEG C of water-baths with 0.1% sodium hydroxide, washes away loose colour on face mask substrate material,
It is neutrality to modulate pH.The dry 4h in baking oven, it is soy molasses biology cellulose film to take out；
(2) prepared by Essence
Water phase (appropriate amount of deionized water, propylene glycol, EDTA-2Na, PVP) is added to magnetic stirring apparatus, starts to stir, add
Appropriate carbomer makes respectively to expect that body is uniformly dispersed in heating process, for use；Oil phase is added in qs glycerin, dimethyl silicone polymer
Pot, makes it completely dissolved, for use；It vacuumizes and oil phase pot material is pumped into emulsification pot, vitamin E is added, homogeneous makes each material
Dispersing and dissolving is uniform, adds methyl hydroxybenzoate, after stirring evenly, filtering and discharging；
(3) prepared by facial mask
By treated, biology cellulose is thin film dipped in the Essence of preparation, then takes out high pressure sterilization and packs low temperature envelope
It deposits, as soy molasses biology cellulose facial mask.
(4) facial mask performance evaluation
1) pH is measured
Using dilution method：Film sample 1g is taken, distilled water 10g is added, is heated to 40 DEG C, is stirred until homogeneous, be cooled to room temperature, is used
PH meter measures；
2) measurement of water-retaining property
Weigh the sample m of a certain amount of moisture content0It is placed in drier (placing the adsorbents such as silica gel) and dries to constant mass, claim
Constant weight quality m after amount sample dryingn, the water retention of sample is calculated, calculation formula is：
Water retention/%=mn/m0× 100,
3) measurement of moisturizing rate
The moisture value x that any cosmetics hand is not smeared in cleaning later is measured using moisture of skin tester0, the face that will configure
Film is applied to the identical position of hand, is removed after applying 10min, and timing (0.5,1,2,4,6 and 10h) measures hand moisture value
xn, moisturizing rate is calculated, calculation formula is：
Moisturizing rate/%=(xn-x0)/x0×100
4) stability test
Heat resistant test is to be placed in 40 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change；Low temperature resistant test is
It is placed in -5 DEG C of storages for 24 hours, has seen whether layering, oil slick phenomenon, color and luster change.
3. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that with big
Beans molasses are that fermentation substrate produces bacterial cellulose facial mask, can reduce cost, and facial mask is degradable, will not cause dirt to environment
Dye.
4. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that with sweet
The Essence that oil, propylene glycol and carbomer etc. are prepared for raw material, it is without any side effects, good whitening, moisturizing, control can be generated
Oil is repaired, convergence pore, the skincare effects such as fine and smooth skin.
5. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that it is
By through seed culture medium (glucose 2%, yeast extract 0.5%, peptone 0.5%, disodium hydrogen phosphate 0.27%, citric acid
0.115%, it is remaining use water polishing, adjust pH=6) amplification after the Xing Shi bacillus of coltfoal between two parties CGMCC12562
(Komagataeibacter intermedius CGMCC12562) is inoculated into soy molasses culture medium (soy molasses solid content
Content 15Brix, corn protein powder 0.15%, zinc sulfate 0.15%, malic acid 0.4%, PH=6 is adjusted), generate biological fiber
Plain basement membrane.
6. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that essence
Formula of liquid is water phase：Deionized water 83.5%, propylene glycol 4%, EDTA-2Na 0.05%, PVP 0.05%, carbomer 0.2%；
Oil phase：Glycerine 5%, dimethyl silicone polymer 5%, vitamin E 2%, methyl hydroxybenzoate 0.2%.
7. a kind of preparation of soy molasses biology cellulose moisture saver mask according to claim 1, it is characterised in that prepare
Facial mask
PH=4.64, water retention 98.35%, moisturizing rate 9.74% stand for 24 hours at -5 DEG C, restore to room in stability test
Temperature is without lamination；It stands for 24 hours, restores to room temperature without lamination, stability is preferable at 40 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369455.9A CN108403474A (en) | 2018-04-24 | 2018-04-24 | A kind of preparation of soy molasses biology cellulose moisture saver mask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810369455.9A CN108403474A (en) | 2018-04-24 | 2018-04-24 | A kind of preparation of soy molasses biology cellulose moisture saver mask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108403474A true CN108403474A (en) | 2018-08-17 |
Family
ID=63136368
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810369455.9A Pending CN108403474A (en) | 2018-04-24 | 2018-04-24 | A kind of preparation of soy molasses biology cellulose moisture saver mask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108403474A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652489A (en) * | 2018-12-21 | 2019-04-19 | 华南协同创新研究院 | A kind of method of the full fermentation bacteria cellulose film of One-step production class containing mushroom extractive from fermentative |
| CN110368315A (en) * | 2019-08-05 | 2019-10-25 | 衡欣实业有限公司 | A kind of probiotics conditioning foster skin facial mask and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101532041A (en) * | 2009-04-24 | 2009-09-16 | 天津实发中科百奥工业生物技术有限公司 | Method for producing bacterial cellulose by taking molasses as raw material |
| WO2016197146A1 (en) * | 2015-06-05 | 2016-12-08 | Cornell University | Modified cellulosic compositions having increased hydrophobicity and processes for their production |
-
2018
- 2018-04-24 CN CN201810369455.9A patent/CN108403474A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101532041A (en) * | 2009-04-24 | 2009-09-16 | 天津实发中科百奥工业生物技术有限公司 | Method for producing bacterial cellulose by taking molasses as raw material |
| WO2016197146A1 (en) * | 2015-06-05 | 2016-12-08 | Cornell University | Modified cellulosic compositions having increased hydrophobicity and processes for their production |
Non-Patent Citations (2)
| Title |
|---|
| 尹园等: "居间驹形氏杆菌发酵大豆糖蜜生产细菌纤维素条件的优化", 《食品科学》 * |
| 陈竞等: "细菌纤维素的制备和应用研究进展", 《纤维素科学与技术》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652489A (en) * | 2018-12-21 | 2019-04-19 | 华南协同创新研究院 | A kind of method of the full fermentation bacteria cellulose film of One-step production class containing mushroom extractive from fermentative |
| CN109652489B (en) * | 2018-12-21 | 2020-12-22 | 华南协同创新研究院 | Method for producing full-fermentation bacterial cellulose membrane containing mushroom fermentation extract by one-step method |
| CN110368315A (en) * | 2019-08-05 | 2019-10-25 | 衡欣实业有限公司 | A kind of probiotics conditioning foster skin facial mask and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103179875B (en) | Cosmetic bio-cellulose mask pack sheet and method for manufacturing same and preparation method thereof | |
| CN108653059A (en) | A kind of probiotics fermention prepares method and the application of the fermentation of seaweed liquid in cosmetics of fermentation of seaweed liquid | |
| CN108220371A (en) | Application with prebiotic function pea peptide preparation method and pea peptide in food | |
| CN115105452B (en) | Black tea fermentation filtrate and preparation method and application thereof | |
| CN108517345A (en) | A kind of method of thermus thermophilus and saccharomycete combined fermentation | |
| CN107080008A (en) | A kind of natural pile fermentation of golden flower Pu'er cooked teas loose tea is grown dim method | |
| KR102284711B1 (en) | Cosmetic composition comprising the sprout fermentation and preparation method thereof | |
| CN110946792A (en) | Quinoa fermented product and application thereof | |
| CN108403474A (en) | A kind of preparation of soy molasses biology cellulose moisture saver mask | |
| CN110235985A (en) | A kind of microbial fermentation processes for eliminating anti-nutritional factors in bean dregs feed | |
| CN116211767B (en) | Preparation method and application of jasmine flower/black tea fermentation filtrate | |
| CN105296541A (en) | Oat fermentation liquor, preparation method thereof and application of oat fermentation liquor as cosmetic raw material | |
| CN106906160A (en) | A kind of synergist and its fermentation process for lactobacillus-fermented | |
| CN107164308A (en) | A kind of cultural method of melanocyte homoepitaxial | |
| CN106120384B (en) | A kind of blue-black chromabacterium biolaceum colouring method of silk fabric | |
| CN110484569A (en) | A kind of meter of source fermentation material and the preparation method and application thereof | |
| CN114621881A (en) | Saccharomyces cerevisiae fermentation liquor and skin care product comprising saccharomyces cerevisiae fermentation liquor | |
| CN109044876A (en) | A kind of water lock moisture-keeping composition rich in scleroglucan | |
| CN113398045A (en) | Anti-aging and relieving aloe fermented product and preparation method and application thereof | |
| CN108570419A (en) | The large-scale preparation method of Paecilomyces lilacinus | |
| CN111454863A (en) | Lactobacillus fermentum and application thereof in preparation of lactobacillus fermentation liquor with anti-aging function | |
| CN107058448A (en) | A kind of fermentation process of high yield bacteria cellulose | |
| CN109971027A (en) | A method of adjusting bacteria cellulose porosity | |
| CN108660096A (en) | A kind of cultural method of amphimicrobian bacillus | |
| CN109010199A (en) | A kind of skin whitening, moisturizing composition and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180817 |
|
| WD01 | Invention patent application deemed withdrawn after publication |