CN108402031A - A kind of CIK cell cryopreservation device - Google Patents
A kind of CIK cell cryopreservation device Download PDFInfo
- Publication number
- CN108402031A CN108402031A CN201810205756.8A CN201810205756A CN108402031A CN 108402031 A CN108402031 A CN 108402031A CN 201810205756 A CN201810205756 A CN 201810205756A CN 108402031 A CN108402031 A CN 108402031A
- Authority
- CN
- China
- Prior art keywords
- flat container
- flat
- container
- communicating pipe
- copper coin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
Abstract
The invention discloses a kind of CIK cell cryopreservation devices, including the identical flat container of multiple sizes, multiple copper coins and a communicating pipe；Multiple flat container stratiforms are superimposed, and the right end of each flat container is connected to a public communicating pipe；It is provided with copper coin between two neighboring flat container, ensures to be in close contact between the upper and lower surface of copper coin and the flat container of upper and lower；Other than the upper surface of the flat container of top layer is provided with opening, remaining all flat container and communicating pipe are closed.CIK cell survival rate higher is frozen using apparatus of the present invention.
Description
Technical field
The present invention relates to a kind of CIK cell cryopreservation devices.
Background technology
CIK is that human peripheral blood single nucleus cell is used cytokine profiles (such as CD 3-resisting monoclonal antibody, IL-2 in vitro
With IFN-γ etc.) a group foreign cell that obtains afterwards for a period of time of co-incubation.Due to this kind of cell simultaneously express CD3+ and
Two kinds of membrane protein molecules of CD56+, therefore the NK cell sample T lymphocytes that are otherwise known as, with the powerful anti-tumor activity of T lymphocytes
With the non-MHC of NK cells is restricted kills tumor advantage.
CIK cell advantage is as follows：1.CIK cell proliferation rates are fast, and antitumor activity cell can be largely proliferated, and cell is lived
Property also greatly enhances.2.CIK cells have the mechanism of identification tumour, act on normal cytotoxic.3. it is wide to kill tumor spectrum, can
It is equally sensitive to multidrug resistant tumour cell for the treatment of the kinds of tumors such as leukaemia, lymthoma, lung cancer, gastric cancer, intestinal cancer.
4. being typical personalized biological treatment mode.After this kind of cell is fed back, moreover it is possible to so that body immunity is improved, generate special
Antivirus action, to imposing dual effect to oncotherapy.5. due to CIK cell be activation autogenous cell, with rise
Come very safe.
Tumor biotherapy is a big hot spot in current tumor research field, and tumor biotherapy includes cell factor, base
Because of the methods for the treatment of, monoclonal antibody, tumor vaccine and adoptive immunotherapy, wherein tumour cell adoptive immunotherapy exists
In occupation of critical role in tumor biotherapy.Adoptive cellular immunotherapy is to study at present more and have been enter into clinical test
Biotherapy method.It is that the self or alloimmune effector cell of Activation In Vitro is transfused to patient, kills patient's body
Tumour cell；Be suitble to the low patient of cellular immune function, as after high-dose chemotherapy, radiotherapy, after bone-marrow transplantation, virus infection damage
Hinder the patient of immunocyte quantity and function.The appearance of cytokine induced kill cell CIK is controlled for tumour adoptive immunity
Treatment brings new hope.CIK cell is to be equal to take the lead within 1991 finding by the Schmidt Wolf of Stanford Univ USA,
It is heterogeneous thin that human peripheral blood mononuclear cell is obtained a group by they afterwards for a period of time with cytokine profiles co-incubation in vitro
Born of the same parents, the cell have co-expressed two kinds of membrane protein molecules of CD3 and CD56, are a kind of strongest tumour lifes of current known killing activity
Object response to treatment cell, which has both the non-MHC of the powerful anti-tumor activity of T lymphocytes and NK cells, and restricted to kill tumor etc. excellent
Point.In conjunction with treatments such as operation excision, intervention, radio frequency, argon helium knifes, the atomic tubercle stove or body that cannot use operation excision can be removed
It is interior to dissipate the oncocyte deposited, it plays an important role in terms of delaying or preventing the transfer or recurrence of tumour；For being partly temporarily not suitable for
It performs an operation, intervene or the tumor patient of other treatments, can also first carry out CIK cell treatment, improve physical function situation, improve
Quality of life strives for other therapy apparatus meetings.
CIK cell clinically apply it is extremely wide, at present clinic CIK cell cryopreservation methods be mainly：
1、 CIK is thin after this kind of cryopreservation methods thaw
The survival rate of born of the same parents is not high, is found through experiments that granulocyte-macrophage colony-producing units CFU-GM, cell viability, CD34+ are thin
Born of the same parents' rate of recovery is not very high, and inventor has been surprisingly found that one of them critically important reason is by long-term experimental study：Such as Fig. 1
It is shown,
Invention content
The purpose of the present invention is to provide a kind of CIK cell cryopreservation devices.
In order to achieve the goal above, technical scheme is as follows：A kind of CIK cell cryopreservation device, it is characterised in that：
Including the identical flat container of multiple sizes, multiple copper coins and a communicating pipe；
Multiple flat container stratiforms are superimposed, the right end of each flat container be connected to one it is public
Communicating pipe；
It is provided with copper coin between two neighboring flat container, ensures the upper and lower surface of copper coin and the flat appearance of upper and lower
It is in close contact between device；
In addition to the upper surface of the flat container of top layer be provided with opening other than, remaining all flat container be connected to
Pipe is closed.
Further more specifical technical solution：It is flat including the first flat container, the second flat container, third
Container, the 4th flat container, the 5th flat container, the 6th flat container, the 7th flat container, the 8th flat appearance
Device；First flat container is located above the second flat container, and the second flat container is located above the flat container of third,
The flat container of third is located above the 4th flat container, and the 4th flat container is located above the 5th flat container, the
Five flat containers are located above the 6th flat container, and the 6th flat container is located above the 7th flat container, and the 7th
Flat container is located above the 8th flat container；Copper coin, copper coin and upper and lower are set between two neighboring flat container
Container be in close contact, all flat containers are connected to the communicating pipe of right end, in addition to the first flat container upper surface
It is outer to be provided with opening, remaining flat container and communicating pipe are closed.
Compared with prior art, beneficial effects of the present invention：Apparatus of the present invention can with copper coin using flat container
To ensure that it is essentially identical that the CIK cell at each position freezes rate, the problem of being not in case shown in Fig. 1 ( ) apparatus of the present invention freeze thaw after CIK cell survival
Rate is higher than traditional common vessel, and granulocyte-macrophage colony-producing units CFU-GM, cell viability, CD34 is found through experiments that
+ cell recoveries are all higher.
Description of the drawings
Fig. 1 is the common vessel that existing cryopreservation methods use；
Fig. 2 is the structural schematic diagram of apparatus of the present invention.
Wherein, 1 it is planar as container；2 be copper coin；3 be communicating pipe.
Specific implementation mode
The invention will be further described below in conjunction with the accompanying drawings.
Embodiment：A kind of CIK cell cryopreservation device, including the first flat container, the second flat container, third are flat
It is shape container, the 4th flat container, the 5th flat container, the 6th flat container, the 7th flat container, the 8th flat
Container；First flat container is located above the second flat container, and the second flat container is located on the flat container of third
Side, the flat container of third are located above the 4th flat container, and the 4th flat container is located above the 5th flat container,
5th flat container is located above the 6th flat container, and the 6th flat container is located above the 7th flat container, the
Seven flat containers are located above the 8th flat container；Copper coin is set between two neighboring flat container, copper coin with up and down
The container of side is in close contact, and all flat containers are connected to the communicating pipe of right end, in addition to the first flat container upper table
Face is provided with outside opening, remaining flat container and communicating pipe are closed.
The operation principle of the present invention：Flat container can ensure in container all CIK cells with a distance from chamber wall all compared with
Closely, at the same use copper coin design can ensure all flat containers chamber wall each position at temperature almost phase
Together, it is added to by the opening of the first flat container upper surface after the CIK cell frozen being needed to be mixed with frozen stock solution in this way
In all flat containers, it is then placed in refrigerator and is frozen using Programmed freezing mode.
Claims (2)
1. a kind of CIK cell cryopreservation device, it is characterised in that：Including the identical flat container of multiple sizes, multiple copper coins and
One communicating pipe；
Multiple flat container stratiforms are superimposed, and the right end of each flat container is connected to a public company
Siphunculus；
Be provided with copper coin between two neighboring flat container, ensure copper coin upper and lower surface and upper and lower flat container it
Between be in close contact；
Other than the upper surface of the flat container of top layer is provided with opening, remaining all flat container and communicating pipe are equal
It is closed.
2. CIK cell cryopreservation device according to claim 1, it is characterised in that：Including the first flat container, second flat
It is the flat container of flat-shaped container, third, the 4th flat container, the 5th flat container, the 6th flat container, the 7th flat
Shape container, the 8th flat container；First flat container is located above the second flat container, and the second flat container is located at
Above the flat container of third, the flat container of third is located above the 4th flat container, and the 4th flat container is located at the
Above five flat containers, the 5th flat container is located above the 6th flat container, and the 6th flat container is located at the 7th
Above flat container, the 7th flat container is located above the 8th flat container；It is set between two neighboring flat container
Set copper coin, the container of copper coin and upper and lower is in close contact, and all flat containers are connected to the communicating pipe of right end, in addition to the
One flat container upper surface is provided with outside opening, remaining flat container and communicating pipe are closed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810205756.8A CN108402031A (en) | 2018-03-13 | 2018-03-13 | A kind of CIK cell cryopreservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810205756.8A CN108402031A (en) | 2018-03-13 | 2018-03-13 | A kind of CIK cell cryopreservation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108402031A true CN108402031A (en) | 2018-08-17 |
Family
ID=63131125
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810205756.8A Pending CN108402031A (en) | 2018-03-13 | 2018-03-13 | A kind of CIK cell cryopreservation device |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101066052A (en) * | 2007-06-18 | 2007-11-07 | 北京锦绣大地农业股份有限公司 | Livestock embryo vitrifying freeze process on metal surface |
| US20080247915A1 (en) * | 2007-04-05 | 2008-10-09 | Genx International, Inc. | Apparatus for the preparation of mammalian specimens for cryopreservation and/or vitrification, and for thawing and using susch specimens |
| CN205378749U (en) * | 2016-02-04 | 2016-07-13 | 重庆市畜牧科学院 | Large capacity tubule sample liquid nitrogen container storage device |
| CN106900694A (en) * | 2017-03-15 | 2017-06-30 | 柳州市妇幼保健院 | The efficient egg mother cell of drawer type or embryo cryopreservation carry bar |
| CN107047535A (en) * | 2016-09-23 | 2017-08-18 | 中国医学科学院整形外科医院（整形外科研究所） | Cell glass frozen cryopreservation device |
-
2018
- 2018-03-13 CN CN201810205756.8A patent/CN108402031A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080247915A1 (en) * | 2007-04-05 | 2008-10-09 | Genx International, Inc. | Apparatus for the preparation of mammalian specimens for cryopreservation and/or vitrification, and for thawing and using susch specimens |
| CN101066052A (en) * | 2007-06-18 | 2007-11-07 | 北京锦绣大地农业股份有限公司 | Livestock embryo vitrifying freeze process on metal surface |
| CN205378749U (en) * | 2016-02-04 | 2016-07-13 | 重庆市畜牧科学院 | Large capacity tubule sample liquid nitrogen container storage device |
| CN107047535A (en) * | 2016-09-23 | 2017-08-18 | 中国医学科学院整形外科医院（整形外科研究所） | Cell glass frozen cryopreservation device |
| CN106900694A (en) * | 2017-03-15 | 2017-06-30 | 柳州市妇幼保健院 | The efficient egg mother cell of drawer type or embryo cryopreservation carry bar |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180817 |
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