CN108398547A - A kind of water silk earthworm is applied to contaminated sediments repairing effect appraisal procedure - Google Patents
A kind of water silk earthworm is applied to contaminated sediments repairing effect appraisal procedure Download PDFInfo
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- CN108398547A CN108398547A CN201810107266.4A CN201810107266A CN108398547A CN 108398547 A CN108398547 A CN 108398547A CN 201810107266 A CN201810107266 A CN 201810107266A CN 108398547 A CN108398547 A CN 108398547A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention discloses a kind of water silk earthworms to be applied to contaminated sediments repairing effect appraisal procedure, includes the following steps：S1 deposits first carry out preculture, then add repair materials, carry out simulation reparation；S2 water silk earthworms are located away from natural sediment, are added to after laboratory cultures in the deposit repaired；After S3 water silk earthworms culture 28 days, the water silk earthworm of survival is obtained by sieving, incubation is without feeding and changes water, is counted finally by water silk earthworm, weigh obtains sediment remediation recruitment evaluation parameter with larva quantity.Water silk earthworm proposed by the present invention is applied to contaminated sediments repairing effect appraisal procedure, is applied to sediment remediation appraisal procedure, can effectively solve the shortcomings of water environment sediment remediation post evaluation method is complicated for operation, cost is excessively high.
Description
Technical field
The present invention relates to biological prosthetic recruitment evaluation technical fields, it particularly relates to which a kind of water silk earthworm is applied to get dirty
Contaminate sediment remediation effect evaluation method.
Background technology
The bed mud of natural water is source and the remittance of pollutant, and polluter type is more contained by existing marine sediments, is led to
Addition repair materials are crossed pollutant can be effectively removed and be controlled.But pollutant kind is various, it can not be single by removing
Pollutant characterizes sediment remediation effect.
Water silk earthworm has survival ability strong as a kind of benthon that heavily contaminated bed mud in river is common, widely distributed etc.
Advantage, growth rate, survival rate and breeding potential are influenced by the comprehensive toxicity of deposit.Therefore, water silk earthworm is applied to bottom
The assessment of mud repairing effect is a research direction.
For the problems in the relevant technologies, currently no effective solution has been proposed.
Invention content
For above-mentioned technical problem in the related technology, the present invention proposes that a kind of water silk earthworm is repaiied applied to heavily contaminated deposit
Multiple effect evaluation method can be used in the repairing effect for characterizing heavily contaminated deposit.
To realize the above-mentioned technical purpose, the technical proposal of the invention is realized in this way：
A kind of water silk earthworm is applied to heavily contaminated sediment remediation effect evaluation method, includes the following steps：
S1. deposit first carries out preculture, then adds repair materials, carries out simulation reparation.
S2. water silk earthworm is located away from natural sediment, is added to after laboratory cultures in the deposit repaired.
S3. after water silk earthworm culture 28 days, pass through the water silk earthworm for sieving and obtaining survival.Incubation is without feeding and changes
Water is counted by water silk earthworm, weigh obtains sediment remediation recruitment evaluation parameter with larva quantity.
Further, the preculture specifically comprises the following steps：Contaminated sediment remediation is put into the appearance of the glass after pickling
Device, is then added overlying water, and overlying water uses the aeration above tap water for 24 hours, wherein the quality of contaminated sediment remediation and overlying water
Than being 1:5, it is cultivated 2 days or more before repair materials are added.
Further, addition repair materials include that activated carbon, charcoal etc. can play fixed work to pollutant in deposit
With equal materials.Adding method includes that nature covering method or warp are manually stirred method.
Further, after simulation is repaired specifically, repair materials are added, culture apparatus is put at room temperature, it is continuous to cultivate
15 days or more.Overlying water is not changed in incubation.
Further, step S2 specifically comprises the following steps：
A:Separation obtains water silk earthworm from low stain deposit.Using 80 mesh nylon mesh screens, water silk earthworm is screened out to come.
B:Screening is obtained in the tap water that water silk earthworm is positioned over after aeration 24, is put in incubated at room temperature, continuously cultivates 15
It, selects adult water silk earthworm, contaminated sediment remediation is added to after weighing.
Further, water silk earthworm described in step A is that type chooses Hope's water silk earthworm, is the resistant kind in common deposit.
Further, water silk earthworm of growing up described in step B is that monomer weight is 5-10 mg, and length is 3-5 cm.It is added to
It weighs before deposit, will be removed on water silk earthworm surface with filter paper before weighing, then weighed with high accuracy balance, obtain
Initial water silk earthworm weight.
Further, the quantity that deposit is added in water silk earthworm described in step B is contaminated sediment remediation, overlying water and water silk
The mass ratio of earthworm is 1:5:2.
Further, particular content described in step S3 includes after culture, being sieved and being deposited by 80 mesh nylon mesh screens
Running water silk earthworm.It weighed, counted to the water silk earthworm adult of survival and larva.
Further repairing effect evaluation parameter includes that survival rate is determined by cultivating front and back water silk earthworm quantity；Biomass
Variation is determined by cultivating front and back water silk earthworm gross mass；Breeding potential by cultivating after water silk earthworm larva quantity than initial water silk earthworm
Dosage.
Beneficial effects of the present invention：
1, water silk earthworm is most common zoobenthos in fresh water, is original work biology in deposit, belongs to Tubificidae, nematode nematode water silk
Earthworm category, thus select water silk earthworm as characterization object it is of low cost, be easy to acquire；
2, for water silk earthworm living environment based on deposit, the upgrowth situation of itself can indicate the quality of growth environment；
3, during river and lake and reservoir sediment remediation, growth rate, survival rate and the breeding potential of water silk earthworm can be used as deposit
Repairing effect identifies index.
Description of the drawings
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the present invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is that a kind of water silk earthworm described according to embodiments of the present invention is applied to contaminated sediments repairing effect appraisal procedure
Flow diagram；
Fig. 2 is the signal of influence of the repair materials addition to water silk earthworm biomass variety described according to embodiments of the present invention one
Figure；
Fig. 3 is the schematic diagram of influence of the repair materials addition to the survival rate of water silk earthworm described according to embodiments of the present invention two；
Fig. 4 is the schematic diagram of influence of the repair materials addition to water silk earthworm breeding yield described according to embodiments of the present invention three.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, the every other embodiment that those of ordinary skill in the art are obtained belong to what the present invention protected
Range.
As shown in Figure 1, a kind of water silk earthworm is commented applied to contaminated sediments repairing effect according to embodiments of the present invention
Estimate method, includes the following steps：
After S1 carry out preculture to deposit, repair materials are added, simulation reparation is carried out,
Contaminated sediment remediation is put into the glass container after pickling by S11, and overlying water is added, cultivate 2 days or more；
S12 adds repair materials into deposit, and the repair materials include activated carbon, charcoal；
Culture apparatus is put at room temperature by S13, continuously cultivate 15 days or more；
Water silk earthworm is added in the deposit repaired by S2；
After S3 was to water silk earthworm culture 28 days, screening obtains the water silk earthworm of survival, wherein incubation is without feeding and changes water.
Preferably, overlying water uses the aeration above tap water for 24 hours, the quality of contaminated sediment remediation and overlying water in S11
Than being 1:5.
Preferably, the addition manner of the repair materials, which includes nature covering method or warp, is manually stirred method.
Preferably, not changing overlying water in S13 incubations.
Further, S2 is specifically included：
S21 uses 80 mesh nylon mesh screens, screening water outlet silk earthworm from low stain deposit；
The water silk earthworm that screening obtains is positioned in the tap water after aeration 24 by S22, is put in incubated at room temperature, is continuously cultivated 15
It, selects adult water silk earthworm, is added in the deposit after having repaired after weighing.
Preferably, the earthworm of water silk described in S21 is Hope's water silk earthworm.
Preferably, the monomer weight for water silk earthworm of growing up described in S22 is 5-10 mg, length is 3-5 cm, and it is heavy to be added to
It weighs before product object, dirt on water silk earthworm surface is removed with filter paper before weighing, is then weighed, is obtained with high accuracy balance
Obtain initial water silk earthworm weight.
Preferably, the quantity that deposit is added in the earthworm of water silk described in S22 is contaminated sediment remediation, overlying water and water silk
The mass ratio of earthworm is 1:5:2.
Step S3 further comprises：After culture, sieve to obtain survival water silk earthworm by 80 mesh nylon mesh screens, to survival
Water silk earthworm adult and larva weighed, count obtain sediment remediation recruitment evaluation parameter.Repairing effect evaluation parameter packet
It includes：Survival rate is determined by the water silk earthworm quantity for cultivating front and back；Biomass variety is determined by the water silk earthworm gross mass for cultivating front and back；
Breeding potential, by cultivating after water silk earthworm larva quantity than initial water silk earthworm dosage.
In order to facilitate understand the present invention above-mentioned technical proposal, below by way of in specifically used mode to the present invention it is above-mentioned
Technical solution is described in detail.
When specifically used, a kind of water silk earthworm according to the present invention is applied to heavily contaminated sediment remediation effect assessment
Method can carry out concrete application according to following examples：
Embodiment one
The deposit 20g for taking certain river heavily contaminated section, is put into glass container, in addition tap water 100 of the aeration after 24 hours
Ml, deposit are mixed 2 days with water, repair materials are added 5 days again.Repair materials additive amount is the 0-15% of deposit dry weight.
Water silk earthworm can be detached from natural water deposit and be obtained, laboratory cultures 30 days or more after separation, then select it is suitable
Water silk earthworm tested, the water silk earthworm monomer mass selected be 8 ± 1 mg；Length is 3-5 cm.
Water silk earthworm is added and carries out culture 28 days, is then detached water silk earthworm deposit with 80 mesh mesh screens.Incubated at room temperature；
Incubation is without feeding and changes water.Biomass variety is determined by cultivating front and back water silk earthworm gross mass.
Embodiment two
Differently with example one, repairing effect index is survival rate, and survival rate is determined by cultivating front and back water silk earthworm quantity.
Embodiment three
Differently with example one, repairing effect index be breeding potential, breeding potential by cultivating after water silk earthworm larva quantity than just
Beginning water silk earthworm dosage.
After above-described embodiment one is by adding external source repair materials, it is not added with water silk earthworm biomass variety rate before repair materials
There is negative growth, when additive amount repair materials are more than 3%, the change rate of water silk earthworm biomass increases.Pass through repair materials
Addition, water silk earthworm growing environment improved, and water silk earthworm growth rate dramatically increases.
After above-described embodiment two is by adding external source repair materials, it is not added with before repair materials that water silk earthworm survival rate is relatively low to be
11%, when additive amount repair materials are more than 10%, water silk earthworm survival rate to 90% or more.Pass through the addition of repair materials, water silk earthworm
Growing environment is improved, and the survival rate of water silk earthworm dramatically increases.
After above-described embodiment three is by adding external source repair materials, water silk earthworm breeding potential is born before being not added with repair materials
Increase, when additive amount repair materials are more than 3%, water silk earthworm breeding potential reaches 10% or more.Pass through the addition of repair materials, water silk
Earthworm growing environment is improved, and the breeding potential of water silk earthworm dramatically increases.
In conclusion index is identified using the growth rate, survival rate and breeding potential of water silk earthworm as sediment remediation effect, it can
Applied to assessment contaminated sediments repairing effect deposit original is not interfered with using deposit original work biology as characterization object
Raw ecological environment；Meanwhile the present invention can also overcome the unicity and one-sidedness of existing sediment remediation Characterization result.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (9)
1. a kind of water silk earthworm is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that include the following steps：
After S1 carry out preculture to deposit, repair materials are added, simulation reparation is carried out,
Contaminated sediment remediation is put into the glass container after pickling by S11, and overlying water is added, cultivate 2 days or more；
S12 adds repair materials into deposit, and the repair materials include activated carbon, charcoal；
Culture apparatus is put at room temperature by S13, continuously cultivate 15 days or more；
Water silk earthworm is added in the deposit repaired by S2；
After S3 was to water silk earthworm culture 28 days, screening obtains the water silk earthworm of survival, wherein incubation is without feeding and changes water.
2. water silk earthworm according to claim 1 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that
Overlying water, which uses, in S11 is aerated the above tap water for 24 hours, and the mass ratio of contaminated sediment remediation and overlying water is 1:5.
3. water silk earthworm according to claim 1 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that institute
The addition manner for stating repair materials includes that nature covering method or warp are manually stirred method.
4. water silk earthworm according to claim 1 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that
Overlying water is not changed in S13 incubations.
5. water silk earthworm according to claim 1 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that S2
It specifically includes：
S21 uses 80 mesh nylon mesh screens, screening water outlet silk earthworm from low stain deposit；
The water silk earthworm that screening obtains is positioned in the tap water after aeration 24 by S22, is put in incubated at room temperature, is continuously cultivated 15
It, selects adult water silk earthworm, is added in the deposit after having repaired after weighing.
6. water silk earthworm according to claim 5 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that
The earthworm of water silk described in S21 is Hope's water silk earthworm.
7. a kind of water silk earthworm according to claim 5 is applied to contaminated sediments repairing effect appraisal procedure, feature exists
In the monomer weight for water silk earthworm of growing up described in S22 is 5-10 mg, and length is 3-5 cm, needs to carry out before being added to deposit
It weighs, dirt on water silk earthworm surface is removed with filter paper before weighing, is then weighed with high accuracy balance, obtain initial water silk earthworm weight
Amount.
8. water silk earthworm according to claim 5 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that
The quantity that deposit is added in the earthworm of water silk described in S22 is that the mass ratio of contaminated sediment remediation, overlying water and water silk earthworm is 1:5:2.
9. water silk earthworm according to claim 1 is applied to contaminated sediments repairing effect appraisal procedure, which is characterized in that step
Rapid S3 further comprises：After culture, sieve to obtain survival water silk earthworm by 80 mesh nylon mesh screens, to the water silk earthworm of survival at
Year and larva are weighed, are counted.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810107266.4A CN108398547A (en) | 2018-02-02 | 2018-02-02 | A kind of water silk earthworm is applied to contaminated sediments repairing effect appraisal procedure |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529099A (en) * | 2014-11-20 | 2015-04-22 | 环境保护部南京环境科学研究所 | Rigid substrate modifier used after riverway or lake dredging, and preparation method and applications thereof |
| CN104878721A (en) * | 2015-04-29 | 2015-09-02 | 暨南大学 | Application of porous ecological concrete system in remediation of heavy metal and/or nitrogen and phosphor polluted sediment |
| CN204613185U (en) * | 2015-05-22 | 2015-09-02 | 环境保护部南京环境科学研究所 | A kind of bottom mud in lake of simulating covers device to bottom-dwelling poisonous effect |
| CN105967471A (en) * | 2016-05-27 | 2016-09-28 | 中国科学院生态环境研究中心 | Method for restoring copper pollution in sediment under condition of bioturbation and application of method |
| CN106771020A (en) * | 2015-11-23 | 2017-05-31 | 南开大学 | A kind of test method for studying Huo Fu water silk earthworm toxicity in sediment-water body |
| CN107273669A (en) * | 2017-05-27 | 2017-10-20 | 水利部交通运输部国家能源局南京水利科学研究院 | The monitoring method of ecological dredging engineering water Effect of Ecological Restoration |
-
2018
- 2018-02-02 CN CN201810107266.4A patent/CN108398547A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529099A (en) * | 2014-11-20 | 2015-04-22 | 环境保护部南京环境科学研究所 | Rigid substrate modifier used after riverway or lake dredging, and preparation method and applications thereof |
| CN104878721A (en) * | 2015-04-29 | 2015-09-02 | 暨南大学 | Application of porous ecological concrete system in remediation of heavy metal and/or nitrogen and phosphor polluted sediment |
| CN204613185U (en) * | 2015-05-22 | 2015-09-02 | 环境保护部南京环境科学研究所 | A kind of bottom mud in lake of simulating covers device to bottom-dwelling poisonous effect |
| CN106771020A (en) * | 2015-11-23 | 2017-05-31 | 南开大学 | A kind of test method for studying Huo Fu water silk earthworm toxicity in sediment-water body |
| CN105967471A (en) * | 2016-05-27 | 2016-09-28 | 中国科学院生态环境研究中心 | Method for restoring copper pollution in sediment under condition of bioturbation and application of method |
| CN107273669A (en) * | 2017-05-27 | 2017-10-20 | 水利部交通运输部国家能源局南京水利科学研究院 | The monitoring method of ecological dredging engineering water Effect of Ecological Restoration |
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