CN108384743A - A kind of preparation method of cereal embryo source active component nano-scale excretion body - Google Patents
A kind of preparation method of cereal embryo source active component nano-scale excretion body Download PDFInfo
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- CN108384743A CN108384743A CN201810176397.8A CN201810176397A CN108384743A CN 108384743 A CN108384743 A CN 108384743A CN 201810176397 A CN201810176397 A CN 201810176397A CN 108384743 A CN108384743 A CN 108384743A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/04—Plant cells or tissues
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
Abstract
The present invention provides a kind of preparation method of cereal embryo source active component nano-scale excretion body, and the preparation method comprises the following steps：(1) acquisition of cereal embryo；(2) degreasing of cereal embryo；(3) cereal blastochyle is produced；(4) ceramic membrane cleans；（5）Micro-filtration degerming；The extraction of supernatant；(6) nanofiltration concentrates；Nanoscale homogenization；（7）The complexing of active constituent and nanoscale film bubble are formed.The present invention also provides a kind of preparation method of cereal embryo source active component nano-scale excretion body, cereal embryo source active constituent excretion bodies to be applied to the treatment of tumour by concentration and particle diameter distribution such as Fig. 5 of nanoscale excretion body.The present invention prepares excretion body using cereal embryo, abundant with raw material sources, at low cost, can mass production the advantages that, so that scale is become a reality using excretion body.
Description
Technical field
The invention belongs to Field of Drug Discovery, and in particular to the preparation and application of nanoscale excretion body.
Background technology
Wheat is sweet in flavor mild-natured, has nourishing heart, kidney-nourishing and blood, four big primary efficacy of invigorating the spleen；Wheat is main grain in the world
The wheat total output of one of food crop, China accounts for No. 1 in the world, and gross annual output amount is 1.10 ten thousand tons.Wheat is wheat seed
Component part, accounts for the 2.5%~3.0% of wheat seed weight, and the glutathione peroxidase in wheat germ in enzyme, is a kind of effect
Fabulous natural, oxidation resistance is 500 times stronger than vitamin E, is a kind of effective efficiency for prolonging and returning aging anti-cancer
The factor.Contained plumule lectin has a variety of biological effects such as antimicrobial and ant- imutagenicity in wheat embryo, with adipocyte
Reaction, there is similar insulin, and glucose oxidase can be activated to reduce blood-sugar content, can induce macrophage dissolving tumour
Cell.Wheat embryo can keep the integrality of erythrocyte, adjust the synthesis of internal compound, and the biology of erythrocyte is promoted to close
At.Promote cellular respiration, protects lung tissue from air pollution.Improve blood circulation, prevents cholesterol and neutral fat from hoarding
In blood, to take precautions against heart disease, the generation of headstroke.
Excretion body（exosomes）It is a kind of double membrane structure that the diameter that can be secreted by various kinds of cell is about 30-100nm
Cup-shaped vesicles.Exosomes can be participated in many important in intercellular trafficking albumen, mRNA, microRNA isoreactivity substance
Physiology, pathologic process, unique effect is by more and more concerns.
At present exosomes mainly from cells and supernatant, tumor tissues the materials such as diffusate in obtain, separation method
Mainly there are filter membrane filtration method, supercentrifugation, sucrose density gradient centrifugation.It is obtained from the sources such as cells and supernatant
Exosome, material source is limited, and material cost is higher, and the exosomes contents obtained are very low, the exosomes after purification at
This higher, this just constrains the large-scale industrial production of exosomes.Therefore, it explores and is carried from the plant material of abundance
Exosomes is taken, can realize production cost reduction and output increased, there is weight for the large-scale application of exosomes
Want meaning.
Invention content
It is abundant, at low cost, mass producible nanoscale excretion body that the object of the present invention is to provide a kind of raw material sources
Preparation method can be applied to the treatment of tumour.
A kind of preparation method of cereal embryo source active component nano-scale excretion body, which is characterized in that the preparation side
Method includes the following steps：
Step 1, the acquisition of cereal embryo：Select good quality wheat（Corn, rice）The complete cereal embryo obtained Deng the de- embryo of machinery；Powder
It is broken to 120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains the plumule dregs of rice；
Step 3, cereal blastochyle is produced：In parts by weight, 10-20 parts of distilled water, homogeneous is added in the cereal embryo obtained in step 2
Obtain cereal blastochyle；
Step 4, ceramic membrane cleans：The cereal blastochyle is removed into residue using 0.22 um ceramic membrane filters, collects the one of filtration
Secondary supernatant；
Step 5, micro-filtration degerming：By a supernatant of the step 4, degerming is removed using 0.22 um ceramic membrane filters, is collected
The secondary supernatant of filtration（Retain 100 nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30 nm reverse osmosis membranes,
5-20 times of concentration, collects the concentrate of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：It is added 2% in the concentrate of step 6（Relative to total volume）Poly- second
Glycol（PEG-400）Or polyethylene glycol（PEG-800）Homogeneous complex reaction 3-5 hours, that is, formed and stablize cereal embryo source and receive
Meter level active constituent excretion body.
Wherein, the plant material is wheat, maize or rice embryo；
Wherein, the dispersibility, physiological inertia（Stabilizer）, nanoscale fruit glaze agent, slow-released carrier be polyethylene glycol（PEG-
400）Or polyethylene glycol（PEG-800）.
Preferably, such as the preparation method of claim 1 cereal embryo source active component nano-scale excretion body, feature exists
In the plant material is wheat, maize or rice embryo；
Preferably, such as the preparation method of claim 1 cereal embryo source active component nano-scale excretion body, which is characterized in that
The dispersibility, physiological inertia（Stabilizer）, nanoscale fruit glaze agent, slow-released carrier be polyethylene glycol（PEG-400）Or poly- second
Glycol（PEG-800）.
Description of the drawings
The concentration and particle diameter distribution of 1 excretion body of Fig. 1 embodiments.
The concentration and particle diameter distribution of 2 excretion body of Fig. 2 embodiments.
The concentration and particle diameter distribution of 3 excretion body of Fig. 3 embodiments.
The concentration and particle diameter distribution of 4 excretion body of Fig. 4 embodiments.
The concentration and particle diameter distribution of Fig. 5 embodiment 1-4 excretion bodies.
Specific implementation mode
Embodiment 1
Step 1, the acquisition of cereal embryo：The complete wheat germ that the de- embryo of the machineries such as selection good quality wheat obtains；Wheat germ 10g is taken to be crushed to
120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains plumule dregs of rice 9g；
Step 3, cereal blastochyle is produced：In parts by weight, select the plumule dregs of rice obtained in rapid 2 that 12 parts of distilled water (108g) are added,
Homogeneous obtains wheat germ suspension；
Step 4, ceramic membrane cleans：The wheat germ suspension is removed into residue using 0.22 um ceramic membrane filters, collects filtration
A supernatant 105g；
Step 5, micro-filtration degerming：By a supernatant of the step 4, degerming is removed using 0.1 um ceramic membrane filters, is collected
The secondary supernatant 103g of filtration（Retain 100nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30nm reverse osmosis membranes,
10 times of concentration or more, collects the concentrate 10g of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：It is added 1% in the concentrate of step 6（Relative to total volume）Poly- second
Glycol（PEG-400）The homogeneous complex reaction of 0.1g 3 hours forms and stablizes wheat germ source nano level active ingredient excretion body.
Pass through nano particle trace analysis technology（NTA）Determine the grain size distributed number of Exosome as implemented illustration 1.
Embodiment 2
A kind of preparation method of cereal embryo source active component nano-scale excretion body, which is characterized in that the preparation method packet
Include following steps：
Step 1, the acquisition of cereal embryo：The complete wheat germ that the de- embryo of the machineries such as selection good quality wheat obtains；Wheat germ 10g is taken to be crushed to
120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains plumule dregs of rice 9g；
Step 3, cereal blastochyle is produced：In parts by weight, select the plumule dregs of rice obtained in rapid 2 that 16 parts of distilled water (144g) are added,
Homogeneous obtains wheat germ suspension；
Step 4, ceramic membrane cleans：The wheat germ suspension is removed into residue using 0.22 um ceramic membrane filters, collects filtration
A supernatant 139g；
Step 5, micro-filtration degerming：By a supernatant of the step 4, degerming is removed using 0.1 um ceramic membrane filters, is collected
The secondary supernatant 135g of filtration（Retain 100nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30nm reverse osmosis membranes,
13 times of concentration or more, collects the concentrate 10g of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：It is added 1.5% in the concentrate of step 6（Relative to total volume）It is poly-
Ethylene glycol（PEG-400）The homogeneous complex reaction of 0.15g 3.5 hours forms and stablizes outside the nano level active ingredient of wheat germ source
Secrete body.Pass through nano particle trace analysis technology（NTA）Determine the grain size distributed number of Exosome as implemented illustration 2.
Embodiment 3
A kind of preparation method of cereal embryo source active component nano-scale excretion body, which is characterized in that the preparation method packet
Include following steps：
Step 1, the acquisition of cereal embryo：The complete wheat germ that the de- embryo of the machineries such as selection good quality wheat obtains；Wheat germ 10g is taken to be crushed to
120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains plumule dregs of rice 9g；
Step 3, cereal blastochyle is produced：In parts by weight, select the plumule dregs of rice obtained in rapid 2 that 18 parts of distilled water (162g) are added,
Homogeneous obtains wheat germ suspension；
Step 4, ceramic membrane cleans：The wheat germ suspension is removed into residue using 0.22 um ceramic membrane filters, collects filtration
A supernatant 156g；
Step 5, micro-filtration degerming：By a supernatant of the step 4, degerming is removed using 0.1 um ceramic membrane filters, is collected
The secondary supernatant 151g of filtration（Retain 100nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30nm reverse osmosis membranes,
15 times of concentration or more, collects the concentrate 10g of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：It is added 1% in the concentrate of step 6（Relative to total volume）Poly- second
Glycol（PEG-800）The homogeneous complex reaction of 0.1g 4 hours forms and stablizes wheat germ source nano level active ingredient excretion body.
Pass through nano particle trace analysis technology（NTA）Determine the grain size distributed number of Exosome as implemented illustration 3.
Embodiment 4
A kind of preparation method of cereal embryo source active component nano-scale excretion body, which is characterized in that the preparation method packet
Include following steps：
Step 1, the acquisition of cereal embryo：The complete wheat germ that the de- embryo of the machineries such as selection good quality wheat obtains；Wheat germ 10g is taken to be crushed to
120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains plumule dregs of rice 9g；
Step 3, cereal blastochyle is produced：In parts by weight, select the plumule dregs of rice obtained in rapid 2 that 20 parts of distilled water (180g) are added,
Homogeneous obtains wheat germ suspension；
Step 4, ceramic membrane cleans：The wheat germ suspension is removed into residue using 0.22 um ceramic membrane filters, collects filtration
A supernatant 176g；
Step 5, micro-filtration degerming：By a supernatant of the step 4, degerming is removed using 0.1 um ceramic membrane filters, is collected
The secondary supernatant 170g of filtration（Retain 100nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30nm reverse osmosis membranes,
17 times of concentration or more, collects the concentrate 10g of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：It is added 1.5% in the concentrate of step 6（Relative to total volume）It is poly-
Ethylene glycol（PEG-800）The homogeneous complex reaction of 0.15g 5 hours forms and stablizes wheat germ source nano level active ingredient excretion
Body.Pass through nano particle trace analysis technology（NTA）Determine the grain size distributed number of Exosome as implemented illustration 4.
Wherein, the plant material is wheat, maize or rice embryo；
Wherein, the dispersibility, physiological inertia（Stabilizer）, nanoscale fruit glaze agent, slow-released carrier be polyethylene glycol（PEG-
400）Or polyethylene glycol（PEG-800）.
It is abundant, at low cost, mass producible nanoscale excretion body that the object of the present invention is to provide a kind of raw material sources
Preparation method can be applied to the treatment of tumour.
The model of film employed in embodiment is not limited to the concrete numerical value being previously mentioned, can be in claim and invention
Arbitrarily change in the pointed corresponding range of speeds in appearance.
What has been described above is only a preferred embodiment of the present invention, it is noted that for those of ordinary skill in the art
For, without departing from the concept of the premise of the invention, various modifications and improvements can be made, these belong to the present invention
Protection domain.
Claims (3)
1. a kind of preparation method of cereal embryo source active component nano-scale excretion body, which is characterized in that the preparation method
Include the following steps：
Step 1, the acquisition of cereal embryo：Select good quality wheat（Corn, rice）The complete cereal embryo obtained Deng the de- embryo of machinery；Powder
It is broken to 120 mesh；
Step 2, the degreasing of cereal embryo：Subcritical abstraction deviates from embryo oil, obtains the plumule dregs of rice；
Step 3, cereal blastochyle is produced：10~20 parts of distilled water, homogeneous is added in the cereal embryo obtained in step 2 in parts by weight
Obtain cereal blastochyle；
Step 4, ceramic membrane cleans：The cereal blastochyle is removed into residue using 0.22 um -0.45 um ceramic membrane filters, is received
Collect a supernatant of filtration；
Step 5, micro-filtration degerming：By a supernatant of the step 4, removed using 0.1 um -0.22 um ceramic membrane filters
The secondary supernatant of filtration is collected in degerming（Retain 100 nm active constituents below）；
Step 6, nanofiltration concentrates：By the secondary supernatant of the step 5, water and other molecules are removed using 30nm reverse osmosis membranes,
5-15 times of concentration, collects the concentrate of filtration（Retain the active constituent of 30 nm or more）；
Step 7, the complexing of active constituent and film bubble are formed：1-5% is added in the concentrate of step 6（Relative to total volume）It is poly-
Ethylene glycol（PEG-400）Or polyethylene glycol（PEG-800）Homogeneous complex reaction 1-5 hours, that is, formed and stablize cereal embryo source
Nano level active ingredient excretion body.
2. such as the preparation method of claim 1 cereal embryo source active component nano-scale excretion body, which is characterized in that the plant
Raw material is wheat, maize or rice embryo.
3. such as the preparation method of claim 1 cereal embryo source active component nano-scale excretion body, which is characterized in that described point
Dissipate property, physiological inertia（Stabilizer）, nanoscale fruit glaze agent, slow-released carrier be polyethylene glycol（PEG-400）Or polyethylene glycol
（PEG-800）.
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| CN201810176397.8A CN108384743A (en) | 2018-03-03 | 2018-03-03 | A kind of preparation method of cereal embryo source active component nano-scale excretion body |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3576844A4 (en) * | 2017-02-01 | 2020-12-23 | Yeditepe Üniversitesi | A product containing plant derived exosomes |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087169A (en) * | 2013-02-05 | 2013-05-08 | 江南大学 | Preparation method of antitumor wheat germ proteins |
| CN103479682A (en) * | 2012-06-14 | 2014-01-01 | 苏州恒宇生物科技有限公司 | Preparation method for plant source active component nano-scale membrane type vesicle |
| WO2016033695A1 (en) * | 2014-09-05 | 2016-03-10 | Exerkine Corporation | Exosome isolation |
| WO2017147719A1 (en) * | 2016-03-04 | 2017-09-08 | Exerkine Corporation | Method for treating neuropathy |
| CN107523536A (en) * | 2017-10-20 | 2017-12-29 | 上海市同济医院 | A kind of extracting method of tissue-derived excretion body and application |
-
2018
- 2018-03-03 CN CN201810176397.8A patent/CN108384743A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103479682A (en) * | 2012-06-14 | 2014-01-01 | 苏州恒宇生物科技有限公司 | Preparation method for plant source active component nano-scale membrane type vesicle |
| CN103087169A (en) * | 2013-02-05 | 2013-05-08 | 江南大学 | Preparation method of antitumor wheat germ proteins |
| WO2016033695A1 (en) * | 2014-09-05 | 2016-03-10 | Exerkine Corporation | Exosome isolation |
| WO2017147719A1 (en) * | 2016-03-04 | 2017-09-08 | Exerkine Corporation | Method for treating neuropathy |
| CN107523536A (en) * | 2017-10-20 | 2017-12-29 | 上海市同济医院 | A kind of extracting method of tissue-derived excretion body and application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3576844A4 (en) * | 2017-02-01 | 2020-12-23 | Yeditepe Üniversitesi | A product containing plant derived exosomes |
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Application publication date: 20180810 |