CN108384732A - A kind of hydrogenlike silicon ion and its application for reducing metrifonate toxicity - Google Patents

A kind of hydrogenlike silicon ion and its application for reducing metrifonate toxicity Download PDF

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Publication number
CN108384732A
CN108384732A CN201810134691.2A CN201810134691A CN108384732A CN 108384732 A CN108384732 A CN 108384732A CN 201810134691 A CN201810134691 A CN 201810134691A CN 108384732 A CN108384732 A CN 108384732A
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Prior art keywords
metrifonate
strain
silicon ion
hydrogenlike silicon
toxicity
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CN201810134691.2A
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CN108384732B (en
Inventor
曹海鹏
张书萌
安健
陈百尧
虞晶晶
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Lianyungang Marine And Fisheries Development Promotion Center
Shanghai Ocean University
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Lianyungang Marine And Fisheries Development Promotion Center
Shanghai Ocean University
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/306—Pesticides
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/34—Organic compounds containing oxygen

Abstract

The present invention relates to a kind of hydrogenlike silicon ion and its applications, the new strains hydrogenlike silicon ion XR12 that inventor screens has very strong tolerance not only for the metrifonate in water environment in other words culture environment of aquatic products, more importantly have the effect of reducing the toxic effect of metrifonate of the water environment in other words in culture environment of aquatic products, can be applied to aquaculture field or water environment treatment field.

Description

A kind of hydrogenlike silicon ion and its application for reducing metrifonate toxicity
Technical field
The present invention relates to a kind of hydrogenlike silicon ion for reducing metrifonate toxicity and its applications, belong to microorganism field.
Background technology
Metrifonate is widely used insecticide in China's aquaculture, is widely used in the anti-of aquatic livestock parasitic disease It controls.However, in practical applications, metrifonate is in alkaline water body, toxicity enhancing, frequently results in aquatic livestock and poisoning thing occur Therefore.In addition to this, the excessive Reusability of metrifonate is also easy to cause its residual in water environment, seriously affects aquatic products Quality safety generates murder by poisoning to aquatile, and then is generated to aquatic ecosystem and human health potentially hazardous.
Therefore, the incidence for how reducing the intoxication accident caused by metrifonate use in aquaculture has important meaning Justice.
While researcher in this field wants to develop the toxicity that can be resistant to metrifonate always, additionally it is possible to drop Microorganism fungus kind that is low or weakening the poisonous effect of metrifonate in water environment.
Invention content
In order to solve the above technical problems, one aspect of the present invention provides a kind of hydrogenlike silicon ion XR12, the hydrogenlike silicon ion XR12 is preserved in China typical culture collection center, and deposit number is CCTCC NO： M2017826.
Another aspect of the present invention additionally provides applications of the above-mentioned hydrogenlike silicon ion XR12 in aquaculture field.
In a preferred embodiment of the invention, the hydrogenlike silicon ion XR12 is in the water environment for reducing aquaculture In metrifonate toxicity application.
Further aspect of the present invention provides applications of the above-mentioned hydrogenlike silicon ion XR12 in water environment treatment field.
In a preferred embodiment of the invention, metrifonate of the hydrogenlike silicon ion XR12 in reducing water environment The application of toxicity.
The present invention provides a kind of new strains --- and hydrogenlike silicon ion XR12, not only for water environment, aquatic products is supported in other words The metrifonate grown in environment has very strong tolerance, it is often more important that in reduction water environment in other words culture environment of aquatic products Metrifonate toxic effect effect, can be applied to aquaculture field or water environment treatment field.
Description of the drawings
Fig. 1 is the photos of the strain X R12 of the present invention under an optical microscope.
Specific implementation mode
Below with reference to specific implementation mode shown in the drawings, the present invention will be described in detail.But these embodiments are simultaneously The present invention is not limited, structure that those skilled in the art are made according to these embodiments, method or functionally Transformation is included within the scope of protection of the present invention.
Example embodiment is described more fully with reference to the drawings.However, example embodiment can be with a variety of shapes Formula is implemented, and is not understood as limited to embodiment set forth herein；On the contrary, thesing embodiments are provided so that the present invention will Fully and completely, and by the design of example embodiment comprehensively it is communicated to those skilled in the art.
Material used in following example, reagent etc., are commercially available unless otherwise specified.Embodiment In particular technique or condition person is not specified, according to technology described in document in the art or condition (such as with reference to J. Pehanorms The works such as Brooker, what Huang Peitang etc. was translated《Molecular Cloning:A Laboratory guide》The third edition, Science Press) or according to product description into Row.
One, the hydrogenlike silicon ion that present inventor's separation screening from natural environment obtains the present invention is described below The operating process of (Rhodobacter sphaeroides) XR12.
1) primary dcreening operation：The aquaculture pond mud (picking up from Qingpu Shanghai farm) that 5g was frequently used to fishing metrifonate, with The sterile ordinary nutritional broth bouillon of 100mL a concentration of 1000mg/L containing metrifonate is added in the conical flask of 250mL and mixes It is even, 25 DEG C of shake culture 18h；Then again in the flat of the sterile ordinary nutrient agar culture medium of a concentration of 1000mg/L containing metrifonate Scribing line isolation and purification is carried out on plate；Several bacterial strains isolated are positioned over the sterile general of the 2000mg/L of concentration containing metrifonate again On logical nutrient agar tablet, if growing state is good, (enemy hundred is reduced with potential as the candidate strain filtered out The strain excellent of worm toxic effect)；
2) morphologic observation of the candidate strain of primary dcreening operation：Then it is sterile general the candidate strain screened to be inoculated in 100 mL In the conical flask of the 250mL of logical nutrient broth medium (being free of metrifonate), what 25 DEG C of shake cultures obtained after 18-24 hours Culture solution is the culture solution of candidate strain.It is flat that the sterile ordinary nutrient agar culture medium of dilution spread may be used in the concentration of bacterial strain Plate method is measured, and simple staining is then used to carry out microexamination to the form of candidate strain.
3) it is further screened by tolerance test：Tolerance of the candidate strain to metrifonate is measured using doubling dilution (using metrifonate to the minimum bactericidal concentration of candidate strain as criterion).By metrifonate solution, (whole mass concentration is 1000mg/mL) be separately added into the test tube containing 2mL sterile vegetative meat soups, until whole mass concentration is respectively 30720,15360, 7680、3840、1920、960、480、240、 120、60、30、15、7.5、3.75、1.88、0.94、0.47、0.24、0.12、 0.06,0.03,0 mg/L, and using the sterile ordinary nutritional broth bouillon of blank as blank control, then inoculation is candidate respectively The culture solution of bacterial strain is to final concentration 5.0 × 105Cfu/mL, in 25 DEG C of constant temperature incubations for 24 hours afterwards observation as a result, asepsis growth, dilute The mass concentration of the maximum metrifonate of degree of releasing is as the maximal tolerable concentration with candidate strain to metrifonate.
By the tolerance test to high concentration metrifonate, filter out with the potential metrifonate toxic effect of reducing Strain excellent XR12.
Two, the qualification process about strain X R12 is as follows：
1) molecular biology identification
According to bacterial 16 S rRNA sequences, Molecular Identification is carried out to strain X R12 using Phylogenetic Analysis.
Specifically, using the bacterial genomes extracts kit of Tiangeng biochemical technology Co., Ltd extraction strain X R12's Genomic DNA；The PCR amplification kit of Tiangeng biochemical technology Co., Ltd is used to expand the 16S rRNA sequences of strain X R12 again Row, wherein it is 5 '-AGAGTTTGATCCTGGCTCAG-3 ' (such as SEQ ID NO.1 institutes to expand the forward primer sequence used Show), reverse primer sequences are：5 '-GGTTACCTTGTTACGACTT-3 ' (as shown in SEQ ID NO.2), concrete operations according to Kit specification carries out.Amplified band carries out cutting glue using the DNA purifying QIAquick Gel Extraction Kit of Tiangeng biochemical technology Co., Ltd Recycling.The sample of recycling send to Shanghai Jie Li Bioisystech Co., Ltd and carries out sequencing.
Determine that the 16S rRNA of strain X R12 are the segment of 1338bp sizes, particular sequence such as SEQ ID by sequencing Shown in NO.3.The 16S rRNA sequences of the sequence and known bacterium in Genbank are carried out similitude to compare, as a result show bacterial strain XR12 has 99%~100% homology with the known bacterial strain that red bacterium belongs to, on this basis, the systematic growth belonged to from red bacterium Tree further identifies that strain X R12 is hydrogenlike silicon ion (Rhodobacter sphaeroides).
2) strain morphology is identified
The thalline of strain X R12 is in rod-shaped, is partly elongated rod shape, morphologically has in ovoid with common hydrogenlike silicon ion Institute is different.
Photos of the strain X R12 under 40 times of light microscopes is referring to Fig. 1.
Three, about the preservation of bacterial strain
The present invention's has the strain X R12 for reducing metrifonate toxic effect, belongs to hydrogenlike silicon ion (Rhodobacter Sphaeroides), preservation, preservation date have been carried out in China typical culture collection center：On December 20th, 2017；It protects Hide number：CCTCC NO：M2017826.
Four, tests of strain X R12 of the present invention with reduction metrifonate toxic effect for the tolerance of metrifonate
It uses doubling dilution to measure candidate strain (to kill the minimum of candidate strain with metrifonate the tolerance of metrifonate Bacteria concentration is as criterion).Metrifonate solution (whole mass concentration is 1000mg/mL) is separately added into containing the sterile battalion of 2mL In the test tube for supporting meat soup, until whole mass concentration is respectively 30720,15360,7680,3840,1920,960,480,240,120, 60,30,15,7.5,3.75,1.88,0.94,0.47,0.24,0.12,0.06,0.03,0mg/L, and it is sterile common with blank Nutrient broth medium as blank control, then respectively the culture solution of inoculation candidate strain to final concentration 5.0 × 105 cfu/ ML, in 25 DEG C of constant temperature incubations for 24 hours afterwards observation as a result, using asepsis growth, the maximum metrifonate of dilution mass concentration as have There is maximal tolerable concentration of the candidate strain to metrifonate.Tolerance with equal conditions lower class ball Rhodobacter strain RED1 to metrifonate Power is as a contrast.
Experimental result is shown in the following table 1.Strain X R12 to the maximum tolerance of metrifonate up to 7680mg/L, with control strain (hydrogenlike silicon ion RED1, from national aquatic animal cause of disease library (Shanghai Ocean University) to the maximum tolerance of metrifonate (3840mg/L) is compared, and strain X R12 of the invention is stronger to the tolerance of metrifonate.
Tolerances of 1 strain X R12 of the table and control strain RED1 to metrifonate
Concentration (mg/L) Strain X R12 Bacterial strain RED1 Concentration (mg/L) Strain X R12 Bacterial strain RED1
0 + + 30 + +
0.03 + + 60 + +
0.06 + + 120 + +
0.12 + + 240 + +
0.24 + + 480 + +
0.47 + + 960 + +
0.94 + + 1920 + +
1.88 + + 3840 + -
3.75 + + 7680 - -
7.5 + + 15360 - -
15 + + 30720 - -
Note：+ indicate bacterium growth；Indicate that bacterium does not grow
Five, the present invention has the Security test for the strain X R12 for reducing metrifonate toxic effect and reduces metrifonate toxicity Effect measurement
First, the safety of candidate strain is measured：Assay method reference《Water quality substance is to fresh-water fishes (zebra fish) acute poison Property assay method》(GB/T13267-1991) is carried out, and specifically, is filling 0.1m3It is prepared according to GB/T13267-1991 Standard dilution water glass aquarium in carry out, be put into 10 tail of healthy zebra fish per cylinder.Test group is often for 24 hours into each aquarium It is 1.0 × 10 that bacteria concentration, which is added,11The culture solution of the strain X R12 of cfu/mL to dosage be 0.1,1,10,100,1000, 10000mg/L, control group do not add any substance.Then after the culture solution for being continuously added to bacterial strain 4 days 1,2,4,8,16, 24,48,72,96h observes the indexs such as fish body respond, travelling ability, respiration capability, records each concentration group zebra fish Mortality and any irregular behavior, calculate strain X R12 to the LC of zebra fish50, each concentration set 3 it is parallel, examination Condition control is in 24~26 DEG C, DO during testing>4mg/L、pH 7.2.
Experimental result is referring to the following table 2, security test results of the strain X R12 to zebra fish.
Acute toxicity results of the 2 strain X R12 of table to zebra fish
It is that 0.1-10000mg/L is continuously added to 1.0 × 10 with dosage11The culture solution of cfu/mL strain Xs R12 4 days, experiment It is abnormal that group and control group zebra fish do not occur fish body slow in reacting, rollover, travelling and respiration capability decrease and death etc. yet Phenomenon, equal well-grown illustrate that bacteria concentration is 1.0 × 101196h-LCs of the strain X R12 of cfu/mL to zebra fish50It is more than 10000mg/L。
Then, metrifonate is measured to zebra fish half lethal dose；Assay method reference《Water quality substance is to fresh-water fishes (spot Horse fish) determination of acute toxicity method》(GB/T13267-1991) is carried out, and specifically, is filling 0.1m3According to GB/ It is carried out in the glass aquarium of standard dilution water prepared by T13267-1991,10 tail of healthy zebra fish is put into per cylinder.Test group Into each aquarium, metrifonate does not add any substance to final concentration 10,20,30,40mg/L, control group, and enemy then is being added After hundred worms 24,48,72,96h the indexs such as fish body respond, travelling ability, respiration capability are observed, record each dense The mortality of degree group zebra fish and any irregular behavior calculate 96 h-LCs of the metrifonate to zebra fish50.During experiment Condition is controlled in 24~26 DEG C, DO>4mg/L、pH 7.2.
Finally, after being added to the strain X R12 with reduction metrifonate toxic effect of the present invention, metrifonate is measured To the half lethal dose of zebra fish, the strain X R12 for thus measuring the present invention reduces the effect of metrifonate toxicity.
Specifically, 0.1m is being filled3According in the glass aquarium of the standard dilution water prepared of GB/T13267-1991 It carries out, 10 tail of healthy zebra fish is put into per cylinder.It is 1.0 × 10 that bacteria concentration is added into each aquarium for test group11The bacterium of cfu/mL The culture solution of strain XR12 to dosage is 10mg/L, is at the same time added in the glass aquarium that strain X R12 is added final concentration of 10,20,30,40mg/L metrifonate, blank control group do not add any substance.Then after on-test 24,48,72,96h The indexs such as fish body respond, travelling ability, respiration capability are observed, record each concentration group zebra fish mortality and Any irregular behavior calculates 96h-LC of the metrifonate to zebra fish of addition strain X R1250.Condition controls during experiment In 24~26 DEG C, DO>4mg/L、pH 7.2.To add the metrifonate of control strain hydrogenlike silicon ion bacterial strain RED1 under equal conditions As a contrast to the acute toxicity test of zebra fish.
Experimental result is shown in the following table 3.
Table 3
It is 1.0 × 10 to add cell concentration11After the strain X R12 culture solutions of cfu/mL, 96h- of the metrifonate to zebra fish LC50For 49.13mg/L, than 96 h-LCs of the metrifonate to zebra fish for being not added with any bacterial strain5096.68% is improved, than adding Add the metrifonate of control strain RED1 to the 96h-LC of zebra fish50391.3% is improved, illustrates that the addition of strain XR12 is obviously dropped Low toxicity of the metrifonate to zebra fish, and control strain RED1 is significantly better than (in addition, control strain RED1 does not have not only Generating reduces the effect of metrifonate toxicity, and also enhances the toxicity of metrifonate, illustrates and not all hydrogenlike silicon ion Bacterial strain all has the function of reducing metrifonate toxicity).
To sum up, the hydrogenlike silicon ion XR12 of the present inventor's screening is not only for water environment aquaculture ring in other words Metrifonate in border has very strong tolerance, it is often more important that with the enemy in reduction water environment in other words culture environment of aquatic products The effect of the toxic effect of hundred worms.
Therefore, hydrogenlike silicon ion XR12 of the invention can be applied to aquaculture field.Preferably, class ball of the invention Red bacterium XR12 can be applied to reduce the toxicity of the metrifonate in the water environment of aquaculture.
The hydrogenlike silicon ion XR12 of the present invention can also be applied to water environment treatment field.Preferably, class ball of the invention Red bacterium XR12 can be applied to reduce the toxicity of the metrifonate in water environment.
It should be appreciated that although this specification is described in terms of embodiments, but not each embodiment only includes one A independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should will say As a whole, the technical solution in each embodiment may also be suitably combined to form those skilled in the art can for bright book With the other embodiment of understanding.
The series of detailed descriptions listed above only for the present invention feasible embodiment specifically Bright, they are all without departing from equivalent implementations made by technical spirit of the present invention not to limit the scope of the invention Or change should all be included in the protection scope of the present invention.
SEQUENCE LISTING
<110>Shanghai Ocean University
Lianyungang ocean promotes center with Fisheries Development
<120>A kind of hydrogenlike silicon ion and its application for reducing metrifonate toxicity
<130> 2018-shou-1
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 20
<212> RNA
<213>It is artificial synthesized
<400> 1
agaguuugau ccuggcucag 20
<210> 2
<211> 19
<212> RNA
<213>It is artificial synthesized
<400> 2
gguuaccuug uuacgacuu 19
<210> 3
<211> 1338
<212> RNA
<213>Hydrogenlike silicon ion
<400> 3
uaccaugcaa gucgagcgaa gucuucggac uuagcggcgg acgggugagu aacgcguggg 60
aacaugccca aagguacgga auagccccgg gaaacuggga guaauaccgu augugcccuu 120
cgggggaaag auuuaucgcc uuuggauugg cccgcguugg auuagguagu ugguggggua 180
auggccuacc aagccgacga uccauagcug guuugagagg augaucagcc acacugggac 240
ugagacacgg cccagacucc uacgggaggc agcagugggg aaucuuagac aaugggcgca 300
agccugaucu agccaugccg cgugaucgau gaaggccuua ggguuguaaa gaucuuucag 360
gugggaagau aaugacggua ccaccagaag aagccccggc uaacuccgug ccagcagccg 420
cgguaauacg gagggggcua gcguuauucg gaauuacugg gcguaaagcg cacguaggcg 480
gacuggaaag ucagggguga aaucccgggg cucaaccccg gaacugccuu ugaaacuccc 540
agucuugagg ucgagagagg ugaguggaau uccgagugua gaggugaaau ucguagauau 600
ucggaggaac accaguggcg aaggcggcuc acuggcucga uacugacgcu gaggugcgaa 660
agcgugggga gcaaacagga uuagauaccc ugguagucca cgccguaaac gaugaaugcc 720
agucgucggg cagcaugcug uucggugaca caccuaacgg auuaagcauu ccgccugggg 780
aguacggccg caagguuaaa acucaaagga auugacgggg gcccgcacaa gcgguggagc 840
augugguuua auucgaagca acgcgcagaa ccuuaccaac ccuugacaug gcgaucgcgg 900
uuccagagau gguuccuuca guucggcugg aucgcacaca ggugcugcau ggcugucguc 960
agcucguguc gugagauguu cgguuaaguc cggcaacgag cgcaacccac guccucaguu 1020
gccagcauuc aguugggcac ucuggggaaa cugccgguga uaagccggag gaaggugugg 1080
augacgucaa guccucaugg cccuuacggg uugggcuaca cacgugcuac aauggcagug 1140
acaauggguu aaucccaaaa agcugucuca guucggauug gggucugcaa cucgacccca 1200
ugaagucgga aucgcuagua aucgcguaac agcaugacgc ggugaauacg uucccgggcc 1260
uuguacacac cgcccgucac accaugggaa uugguucuac ccgaaggcgg ugcgccaacc 1320
ucgcaagagg aggcagcg 1338
SEQUENCE LISTING
<110>Shanghai Ocean University
Lianyungang ocean promotes center with Fisheries Development
<120>A kind of hydrogenlike silicon ion and its application for reducing metrifonate toxicity
<130> 2018-shou-1
<160> 3
<170> PatentIn version 3.3
<210> 1
<211> 20
<212> RNA
<213>It is artificial synthesized
<400> 1
agaguuugau ccuggcucag 20
<210> 2
<211> 19
<212> RNA
<213>It is artificial synthesized
<400> 2
gguuaccuug uuacgacuu 19
<210> 3
<211> 1338
<212> RNA
<213>Hydrogenlike silicon ion
<400> 3
uaccaugcaa gucgagcgaa gucuucggac uuagcggcgg acgggugagu aacgcguggg 60
aacaugccca aagguacgga auagccccgg gaaacuggga guaauaccgu augugcccuu 120
cgggggaaag auuuaucgcc uuuggauugg cccgcguugg auuagguagu ugguggggua 180
auggccuacc aagccgacga uccauagcug guuugagagg augaucagcc acacugggac 240
ugagacacgg cccagacucc uacgggaggc agcagugggg aaucuuagac aaugggcgca 300
agccugaucu agccaugccg cgugaucgau gaaggccuua ggguuguaaa gaucuuucag 360
gugggaagau aaugacggua ccaccagaag aagccccggc uaacuccgug ccagcagccg 420
cgguaauacg gagggggcua gcguuauucg gaauuacugg gcguaaagcg cacguaggcg 480
gacuggaaag ucagggguga aaucccgggg cucaaccccg gaacugccuu ugaaacuccc 540
agucuugagg ucgagagagg ugaguggaau uccgagugua gaggugaaau ucguagauau 600
ucggaggaac accaguggcg aaggcggcuc acuggcucga uacugacgcu gaggugcgaa 660
agcgugggga gcaaacagga uuagauaccc ugguagucca cgccguaaac gaugaaugcc 720
agucgucggg cagcaugcug uucggugaca caccuaacgg auuaagcauu ccgccugggg 780
aguacggccg caagguuaaa acucaaagga auugacgggg gcccgcacaa gcgguggagc 840
augugguuua auucgaagca acgcgcagaa ccuuaccaac ccuugacaug gcgaucgcgg 900
uuccagagau gguuccuuca guucggcugg aucgcacaca ggugcugcau ggcugucguc 960
agcucguguc gugagauguu cgguuaaguc cggcaacgag cgcaacccac guccucaguu 1020
gccagcauuc aguugggcac ucuggggaaa cugccgguga uaagccggag gaaggugugg 1080
augacgucaa guccucaugg cccuuacggg uugggcuaca cacgugcuac aauggcagug 1140
acaauggguu aaucccaaaa agcugucuca guucggauug gggucugcaa cucgacccca 1200
ugaagucgga aucgcuagua aucgcguaac agcaugacgc ggugaauacg uucccgggcc 1260
uuguacacac cgcccgucac accaugggaa uugguucuac ccgaaggcgg ugcgccaacc 1320
ucgcaagagg aggcagcg 1338

Claims (5)

1. a kind of hydrogenlike silicon ion XR12, hydrogenlike silicon ion XR12 are preserved in China typical culture collection center, preservation is compiled Number be CCTCC NO：M2017826.
2. hydrogenlike silicon ion XR12 as described in claim 1 is in the application of aquaculture field.
3. application as claimed in claim 2, it is characterised in that：The hydrogenlike silicon ion XR12 is in the water environment for reducing aquaculture In metrifonate toxicity application.
4. hydrogenlike silicon ion XR12 as described in claim 1 is in the application in water environment treatment field.
5. application as claimed in claim 4, it is characterised in that：Metrifonate of the hydrogenlike silicon ion XR12 in reducing water environment The application of toxicity.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111471622A (en) * 2020-04-21 2020-07-31 上海海洋大学 Fermentation production method of bdellovibrio bacteriovorus by using rhodobacter sphaeroides XR12 as host bacteria
CN114317332A (en) * 2021-12-16 2022-04-12 上海海洋大学 Aerobic photosynthetic bacteria and application thereof

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