CN108379654A - A kind of more gradients carry the preparation method of concentration artificial bone scaffold - Google Patents
A kind of more gradients carry the preparation method of concentration artificial bone scaffold Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/20—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
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- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
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- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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Abstract
The present invention proposes that a kind of more gradients carry the preparation method of concentration artificial bone scaffold, after the different fibroin microsphere powder for carrying concentration are mixed with biogum respectively, viscous liquid is added separately in the different liquid storage cylinder of biological D extrusion molding machine, using hydroxyapatite as main raw material(s), by the threedimensional model imported in advance, required more gradients are printed layer by layer and carry concentration artificial bone scaffold.The artificial bone scaffold preparation method that this invention provides can be according to the self-demand of sufferer, using the configuration of more gradient concentration drugs, make the more gentle of the drug release in artificial bone, increase the time of medicine effective concentration release, the drug of more gradient concentrations can also be selectively sprayed to the privileged site of artificial bone, targeting of the realization drug in wound.
Description
Technical field
The present invention relates to the technical fields of medical artificial bone transplantation material, and in particular to a kind of drug packet of more gradient concentrations
The preparation method of the artificial bone scaffold buried.
Background technology
In field of orthopaedics, the bone defect caused by many reasons such as severe trauma, bone tumour, osteomyelitis is very common.
Traumatic bone damage repair process in, easily cause infection, substitute bone collection success or not with whether infect it is closely related.It is infectious
The treatment of bone defect is due to should repair bone defect position, and prevention infection, is institute on Orthopedic Clinical all the time again
The problem faced.Bacterium forms biomembrane in implant surfaces and makes simple bone grafting operation due to infection is not easy to control after infection
Failure, therefore patient tends not to carry out one-stage bone grafting, and must thorough debridement, wait for infective agent elimination, wound healing, blood vessel weight
The second stage of bone grafting repairing bone defect is carried out after building again.This therapy had both increased the pain of patient, also extended treatment time.
In view of the anti-infective characteristic needed for bone holder, antibiotic is added in nano bone to the wind that can effectively reduce infection
Danger.Carrier of the artificial bone as osteoblast plays and provides sufficient nutriment and living space for Oesteoblast growth
Important function, the drug-loaded artificial bone with anti-infective characteristic can reduce patient's infection risk, by the characteristic of nano material, both
It can guarantee the mechanical property of bone holder, and can reach the slow release effect of drug in vivo.
Ideal artificial bone renovating material, which sums up the characteristic that should have, to be had：(1) good biocompatibility with it is weak
Immunological rejection；(2) no inflammation generates, is non-toxic；(3) osteoconductive and self-bone grafting reproducibility；(4) certain machinery is strong
Degree；(5) drug slow release；(6) aperture appropriate and porosity；(7) biological degradability, it is long that degradation speed is no more than new bone
The bone tissue reparation speed entered；(8) plasticity：It can cure in situ, hardening time is moderate, and can coagulate at a lower temperature
Gu heat release is few, not damaged to bone tissue, and can be prefabricated into required arbitrary shape.
Two kinds of the main having time control at present of the control method for releasing of drug and distributed controll.Wherein, time control be for
Realization slow release, allows medicament to the sustained release in one section of long time.The mechanism of time control includes that degradation is controlled
Releasing mechanism processed and diffusion control releasing mechanism.Degradation Control releasing mechanism refers to that drug is constantly released with the degradation of material
It releases, drug release rate is decided by the degradation speed of material；Diffusion control releasing mechanism is to utilize pharmaceutical carrier and body
Drug concentration difference realize that the continuous release of drug, concentration difference can reduce with the increase of drug release degree, therefore this
The maximum feature of one releasing mechanism is that rate of release is gradually reduced.Certainly, there is also both the above control releasing mechanisms to mix
Drug controlled release mode.Distributed controll is to realize that drug in the targeting of wound, makes drug in wound location
Concentration control is in therapeutic domain, and at other positions, control drug concentration is less than drug intoxicating minimum concentration.
Fibroin albumen is the protein extracted from silk, has excellent mechanical performance, good biocompatibility can
The properties such as biodegradable and portable related chemical functional group.Mechanical properties, mechanical modulus, fracture energy, elongation
This three's of rate is well balanced so that fibroin has good mechanical strength and toughness of material, can regenerate and be processed into difference
Product, such as film, porous support.Fibroin ultra microstructure can be well adapted for the injected organism tissue of human body, be widely used in hand
Art suture, artificial skin, slow releasing carrier of medication etc..Using the fibroin microsphere that fibroin albumen is prepared as raw material, there is specific surface area
Greatly, good biocompatibility, it is biodegradable the advantages that, be increasingly becoming a kind of important pharmaceutical carrier.
Vancomycin is glycopeptide antibiotic, to many gram-positive bacterias, such as staphylococcus aureus, has very strong kill
Bacterium acts on, and has been applied to multiple clinical fields.VCM is presently believed to be most effective anti-Staphylococcus aureus infection
With the drug for the treatment of.
Report there is no to be mixed with drug-loaded artificial bone holder with biological glue using the fibroin microsphere for carrying vancomycin at present,
Simultaneously according to the actual conditions of sufferer, the artificial bone scaffold that more gradients carry concentration is made, reaches drug release and distribution for a long time
The effect of control.
Invention content
The object of the present invention is to provide a kind of good biocompatibility, mechanical property is good, degradable, slow-release time and direction can
Control and more gradient concentration drug-loaded artificial bone holders with anti-infective effectiveness.Using powder gluing technology, by the hydroxyl of Nano grade
Base apatite, micron-sized polycaprolactone are mutually mixed the uniformly raw material as bone holder, utilize computer disposal artificial bone
Scaffold three-dimensional model makes every layer of powder cohere, obtains with being mixed with biological adhesive containing the different silk fibroin solutions for carrying concentration
To a kind of bone stent model carrying medicine with anti-infective characteristic and gradient.
The technical scheme is that：
A kind of more gradients carry the preparation method of concentration artificial bone scaffold, it is characterised in that：Include the following steps：
Step 1：According to molar ratio 8：2：1 configuration calcium chloride-alcohol-water ternary solution；Fibroin albumen is obtained, and according to three
First solution is 10 with fibroin albumen quality ratio：1 is matched；Fibroin albumen is placed in ternary solution, and is stirred at 80 DEG C
At least 30min is dissolved, mixed solution is obtained；Mixed solution after cooling is packed into bag filter, is dialysed in distilled water at least 3 days
To remove calcium chloride, silk fibroin solution is obtained using filter；
Step 2：It is 1 by vancomycin and fibroin albumen quality score:10、1:20、1:Fibroin is added in vancomycin by 30
In solution, at least 30min is stirred at normal temperatures, obtains the load medicine silk fibroin solution of three kinds of various concentrations；
Step 3：Ethyl acetate, and ethyl acetate by volume are added into container：Span80=50:1 adds into container
Enter suitable Span80, after the two is stirred at least 15min under room temperature, adjusts agitator speed to 600~700rpm, and
Ethyl acetate by volume：Silk fibroin solution=50:Suitable silk fibroin solution is instilled stirring by 1 dropwise with the speed of 0.4ml/min
Mixed solution in, continue stirring until few 2h, obtain the suspension containing drug bearing microsphere；
Step 4：Step 3 is repeated, the drug bearing microsphere suspension of three kinds of various concentrations is obtained；To drug bearing microsphere suspension stone
Oily ether, isopropanol and ethyl alcohol wash three times successively, and 12h is lyophilized under the conditions of -50 DEG C by freeze drier, collects three kinds of differences
The drug bearing microsphere of concentration；
Step 5：The drug bearing microsphere for three kinds of various concentrations that step 4 is obtained respectively with biological glue by volume 5:1 is mixed
Three kinds of mixture of viscous form are obtained after conjunction, then are separately added into the curing accelerator polyamide and 6.0% glue of 4.0% glue volume
The plasticizer P BMA of volume, is added separately to after mixing in liquid storage cylinder A, B and C of 3D single lead screw ex truding briquetting machines；
Step 6：Be 100 ± 30 μm poly- in oneself by hydroapatite particles that grain size is 100 ± 10nm sizes and grain size
Ester particle is 1 according to mass ratio:4 obtained mixing materials；
Step 7：Artificial bone scaffold threedimensional model is established, and hierarchy slicing processing is carried out to threedimensional model, layering is obtained and cuts
Face data；Corresponding load concentration gradient data is imported into layering cross-section data；
Step 8：The mixing material that on forming worktable prepared by uniform layer overlay step 6；According to point in step 7
Layer cross section data and load concentration gradient data, controlling corresponding three kinds of different nozzles for carrying concentration in 3D single lead screw ex truding briquetting machines will walk
The three kinds of drug-loaded biological glue prepared in rapid 5 are sprayed onto in mixing material, and biological glue average dose is 0.0004ml/mm2；
Step 9：Step 8 is repeated, until all layerings printing finishes, holder completes；Holder is taken out, holder is carried out
Strengthen, grinding process.
Further preferred embodiment, a kind of preparation method of more gradients load concentration artificial bone scaffolds, feature exist
In：The process of acquisition fibroin albumen is in step 1：
A certain amount of silk is weighed, is cleaned to remove impurity with distilled water, is dried under the conditions of 45~55 DEG C in drying box
It is dry；Configuration quality score is 0.02% sodium carbonate liquor, and sodium carbonate liquor is heated to 100 DEG C, by the silk after drying
It puts into sodium carbonate liquor, after boiling at least 0.5h, is fully rinsed with distilled water thoroughly to remove silk gum, fibroin is obtained after drying
Albumen.
Advantageous effect
(1) artificial bone scaffold of the invention uses hydroxyapatite as raw material, and mechanical property is good and life is utilized
The degradable property of object microballoon, this makes patient take out implantation bone without second operation, alleviates the pain of patient.The present invention's
Drug-loaded artificial bone holder makes bone holder have certain anti-infection property, effectively reduces or avoid two by the way that antibiotic is added
The possibility of subinfection.
(2) the shortcomings of general biological adhesive has viscosity low, calking poor performance, often cannot due to trickling the reason of
Porous surface is bonded, and brittleness is larger after solidification.In view of the foregoing drawbacks, the present invention sprays solidification in body surface and promotes
Agent, the plasticizer agent of biological adhesive and thickening toughening make it be sufficiently mixed, and pass through and the biological gluing of curing accelerator adjustment is added
The hardening time of agent to achieve the effect that biological adhesive thickening toughening and do in wink, and then increases bioceramic branch
The intensity and reliability of frame.
(3) drug-loaded artificial bone holder of the invention is added dropwise the different biological glues for carrying concentration by being mixed with, is formed different
The drug-loaded artificial bone of gradient concentration uses outside low concentration, the gradient of inside high concentration during artificial bone is gradually degraded
Variation tendency prevents the effect of infection to reach effective sterilizing, in addition to this it is also possible that drug is more permanent gentle
Release increases the time of medicine effective concentration release.
(4) drug-loaded artificial bone of the invention carries out distributed controll by the drug of different gradient concentrations, in wound location
Make drug concentration control in therapeutic domain, control drug concentration is less than drug intoxicating minimum concentration at other positions, realizes drug
Targeting in wound.
This method establishes CAD model according to sufferer individual character first, which is imported three-dimensional printer, will contain not
Fibroin microsphere with concentration vancomycin is mixed with biological glue, and it is mixed then to spray biological adhesive bonding by three-dimensional printer
Ball is closed, realizes the preparation of artificial bone scaffold.
Production method of the present invention is simple, and raw material are easily obtained, and mechanical property is good, is more similar to nature bone holder work
With.The present invention solves the problems, such as artificial bone scaffold and biocompatible, degradability on traditional preparation methods, while carrying medicine silk
The addition of plain microballoon solves the problems, such as the easy infection in bone grafting link, and the artificial bone plant of concentration is carried containing different gradients
After entering, the time of the release that drug can be made gentler, release is more permanent, in addition to this it is possible to realize that drug exists
The targeting of wound enables biological tissue faster to depend on bone grafting growth, there is higher appreciation rate.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obviously, or practice through the invention is recognized.
Specific implementation mode
The embodiment of the present invention is described below in detail, the embodiment is exemplary, it is intended to for explaining the present invention, and
It is not considered as limiting the invention.
A kind of more gradients carry the preparation method of concentration artificial bone scaffold, include the following steps：
Step 1：A certain amount of silk is weighed, is cleaned to remove impurity, in 45~55 DEG C of items in drying box with distilled water
8h is dried under part.The sodium carbonate liquor that configuration quality score is 0.02%, 100 are heated in electromagnetic oven by sodium carbonate liquor
DEG C, by the silk input sodium carbonate liquor after drying, 0.5h is boiled, is fully rinsed with distilled water thoroughly to remove silk gum, is dried
Fibroin albumen is obtained after dry.
According to molar ratio 8：2：1 configuration calcium chloride-alcohol-water ternary solution；Fibroin albumen is obtained, and according to ternary solution
It is 10 with fibroin albumen quality ratio：1 is matched；Fibroin albumen is placed in ternary solution, and the stirring and dissolving at 80 DEG C
30min obtains mixed solution；Mixed solution after cooling is packed into bag filter, 3 days are dialysed in distilled water to remove chlorination
Calcium obtains silk fibroin solution using filter.
Step 2：It is 1 by vancomycin and fibroin albumen quality score:10、1:20、1:Fibroin is added in vancomycin by 30
In solution, magnetic agitation 30min, obtains the load medicine silk fibroin solution of three kinds of various concentrations at normal temperatures.
Step 3：Ethyl acetate is added into beaker, pipette ethyl acetate by volume is used in combination：Span80=50:1 to
Suitable Span80 is added in beaker, after the two is stirred 15min under room temperature, adjusting magnetic stirring apparatus rotating speed to 600~
700rpm, and respectively with syringe ethyl acetate by volume：Silk fibroin solution=50:1 by suitable silk fibroin solution with 0.4ml/
The speed of min is instilled dropwise in the mixed solution of stirring, persistently stirs 2h, obtains the suspension containing drug bearing microsphere.
Step 4：Step 3 is repeated, the drug bearing microsphere suspension of three kinds of various concentrations is obtained；To drug bearing microsphere suspension stone
Oily ether, isopropanol and ethyl alcohol wash three times successively, and 12h is lyophilized under the conditions of -50 DEG C by freeze drier, collects three kinds of differences
The drug bearing microsphere of concentration.
Step 5：The drug bearing microsphere for three kinds of various concentrations that step 4 is obtained respectively with biological glue by volume 5:1 is mixed
Three kinds of mixture of viscous form are obtained after conjunction, then are separately added into the curing accelerator polyamide and 6.0% glue of 4.0% glue volume
The plasticizer P BMA of volume, is added separately to after mixing in liquid storage cylinder A, B and C of 3D single lead screw ex truding briquetting machines.
Step 6：Be 100 ± 30 μm poly- in oneself by hydroapatite particles that grain size is 100 ± 10nm sizes and grain size
Ester particle is 1 according to mass ratio:4 obtained mixing materials.
Step 7：Using bone tissue at Micro-CT scanning injury of human, injured bone image data is obtained；It will obtain
Injury region bone tissue image data import Mimics softwares, the bone threedimensional model at reconstruct injury of human position, with STL formats
Import 3D bone shaping systems；Computer disposal artificial bone scaffold three-dimensional CAD model, spacing is sequentially divided by it from bottom to up
Two dimension N parts of the sectional view of layering of 0.6mm, obtains layering cross-section data, corresponding load concentration gradient data is imported and is layered
In cross-section data, then cross-section data will be layered and imported in three-dimensional printer.
Step 8：Start three-dimensional printer, i=1 is set；On forming worktable prepared by uniform layer overlay step 6
Mixing material；According to the layering cross-section data and load concentration gradient data in step 7, control corresponding three in 3D single lead screw ex truding briquetting machines
Prepared in step 5 three kinds of drug-loaded biological glue are sprayed onto in mixing material by the different nozzles for carrying concentrations of kind, biological glue
Average dose is 0.0004ml/mm2So that corresponding load medicine gradient, after having printed one layer, i=i+1 are formed from outside to inside；
Step 9：Step 8 is repeated, until all layerings printing finishes, holder completes；Holder is taken out, holder is carried out
Strengthen, grinding process.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case of can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (2)
1. a kind of more gradients carry the preparation method of concentration artificial bone scaffold, it is characterised in that：Include the following steps：
Step 1：According to molar ratio 8：2：1 configuration calcium chloride-alcohol-water ternary solution；Fibroin albumen is obtained, and molten according to ternary
Liquid is 10 with fibroin albumen quality ratio：1 is matched；Fibroin albumen is placed in ternary solution, and the stirring and dissolving at 80 DEG C
At least 30min obtains mixed solution；Mixed solution after cooling is packed into bag filter, at least 3 days are dialysed in distilled water to remove
Calcium chloride is removed, silk fibroin solution is obtained using filter；
Step 2：It is 1 by vancomycin and fibroin albumen quality score:10、1:20、1:Silk fibroin solution is added in vancomycin by 30
In, at least 30min is stirred at normal temperatures, obtains the load medicine silk fibroin solution of three kinds of various concentrations；
Step 3：Ethyl acetate, and ethyl acetate by volume are added into container：Span80=50:1 be added into container it is suitable
The Span80 of amount after the two is stirred at least 15min under room temperature, adjusts agitator speed to 600~700rpm, and press body
Product compares ethyl acetate：Silk fibroin solution=50:1 instills suitable silk fibroin solution with the speed of 0.4ml/min the mixed of stirring dropwise
It closes in solution, continues stirring until few 2h, obtain the suspension containing drug bearing microsphere；
Step 4：Step 3 is repeated, the drug bearing microsphere suspension of three kinds of various concentrations is obtained；To drug bearing microsphere suspension oil
Ether, isopropanol and ethyl alcohol wash three times successively, and 12h is lyophilized under the conditions of -50 DEG C by freeze drier, it is dense to collect three kinds of differences
The drug bearing microsphere of degree；
Step 5：The drug bearing microsphere for three kinds of various concentrations that step 4 is obtained respectively with biological glue by volume 5:After 1 mixing
Three kinds of mixture of viscous form are obtained, then are separately added into the curing accelerator polyamide and 6.0% glue volume of 4.0% glue volume
Plasticizer P BMA, be added separately to after mixing in liquid storage cylinder A, B and C of 3D single lead screw ex truding briquetting machines；
Step 6：The polycaprolactone for being 100 ± 30 μm with grain size by the hydroapatite particles that grain size is 100 ± 10nm sizes
Grain is 1 according to mass ratio:4 obtained mixing materials；
Step 7：Artificial bone scaffold threedimensional model is established, and hierarchy slicing processing is carried out to threedimensional model, obtains layering number of cross-sections
According to；Corresponding load concentration gradient data is imported into layering cross-section data；
Step 8：The mixing material that on forming worktable prepared by uniform layer overlay step 6；It is cut according to the layering in step 7
Face data and load concentration gradient data control and correspond to three kinds of different nozzles for carrying concentration in 3D single lead screw ex truding briquetting machines by step 5
Three kinds of drug-loaded biological glue of middle preparation are sprayed onto in mixing material, and biological glue average dose is 0.0004ml/mm2；
Step 9：Step 8 is repeated, until all layerings printing finishes, holder completes；Holder is taken out, holder is carried out strong
Change, grinding process.
2. a kind of more gradients carry the preparation method of concentration artificial bone scaffold according to claim 1, it is characterised in that：
The process of acquisition fibroin albumen is in step 1：
A certain amount of silk is weighed, is cleaned to remove impurity with distilled water, is dried under the conditions of 45~55 DEG C in drying box；Match
It sets mass fraction and is 0.02% sodium carbonate liquor, and sodium carbonate liquor is heated to 100 DEG C, the silk after drying is put into carbon
In acid sodium solution, after boiling at least 0.5h, is fully rinsed with distilled water thoroughly to remove silk gum, fibroin albumen is obtained after drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810423739.1A CN108379654A (en) | 2018-05-06 | 2018-05-06 | A kind of more gradients carry the preparation method of concentration artificial bone scaffold |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810423739.1A CN108379654A (en) | 2018-05-06 | 2018-05-06 | A kind of more gradients carry the preparation method of concentration artificial bone scaffold |
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| CN108379654A true CN108379654A (en) | 2018-08-10 |
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| CN201810423739.1A Pending CN108379654A (en) | 2018-05-06 | 2018-05-06 | A kind of more gradients carry the preparation method of concentration artificial bone scaffold |
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