CN108362784A - A kind of method of bee honey and Apis mellifera honey in discriminating - Google Patents
A kind of method of bee honey and Apis mellifera honey in discriminating Download PDFInfo
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- CN108362784A CN108362784A CN201810008004.2A CN201810008004A CN108362784A CN 108362784 A CN108362784 A CN 108362784A CN 201810008004 A CN201810008004 A CN 201810008004A CN 108362784 A CN108362784 A CN 108362784A
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- 235000012907 honey Nutrition 0.000 title claims abstract description 117
- 241000256844 Apis mellifera Species 0.000 title claims abstract description 100
- 238000000034 method Methods 0.000 title claims abstract description 18
- -1 C37 alkane Chemical class 0.000 claims abstract description 16
- 150000001335 aliphatic alkanes Chemical class 0.000 claims abstract description 16
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000003208 petroleum Substances 0.000 claims abstract description 7
- 238000004949 mass spectrometry Methods 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 24
- 239000000284 extract Substances 0.000 claims description 21
- 229910052757 nitrogen Inorganic materials 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 7
- NLAGNNORBYGNAV-UHFFFAOYSA-N isotriacontane Natural products CCCCCCCCCCCCCCCCCCCCCCCCCCCC(C)C NLAGNNORBYGNAV-UHFFFAOYSA-N 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000010792 warming Methods 0.000 claims description 6
- 239000004215 Carbon black (E152) Substances 0.000 claims description 4
- 230000009514 concussion Effects 0.000 claims description 4
- 229930195733 hydrocarbon Natural products 0.000 claims description 4
- 239000012159 carrier gas Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 150000002430 hydrocarbons Chemical class 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000001819 mass spectrum Methods 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000012074 organic phase Substances 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 239000012224 working solution Substances 0.000 claims description 3
- 238000004817 gas chromatography Methods 0.000 claims 1
- 239000004615 ingredient Substances 0.000 abstract description 4
- 235000013379 molasses Nutrition 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 238000010348 incorporation Methods 0.000 abstract description 2
- 238000012545 processing Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 abstract 1
- 238000000605 extraction Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 229940109850 royal jelly Drugs 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 241000241413 Propolis Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 229940069949 propolis Drugs 0.000 description 2
- 241000256846 Apis cerana Species 0.000 description 1
- 241000358063 Apis cerana cerana Species 0.000 description 1
- 241000256843 Apis mellifera ligustica Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000009341 apiculture Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 238000012850 discrimination method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N2030/062—Preparation extracting sample from raw material
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Jellies, Jams, And Syrups (AREA)
Abstract
Both a kind of method that the present invention provides bee honey and Apis mellifera honey in discriminating, achieve the purpose that differentiate according to alkane component difference in two kinds of honey.Gaschromatographic mass spectrometry detection is carried out after honey sample petroleum ether extraction, bee honey and Apis mellifera honey have differences on alkane component in as a result showing, middle bee honey contains C37 alkane, and Apis mellifera honey contains C31 alkane.Determine that this difference ingredient is only related with honeybee kind source, eliminates influence of the plant source to discriminating after great amount of samples tests and analyzes.Otherness alkane component is stabilized in honey, is not influenced by concentration processing and shelf life.The method of the present invention reasonable design, convenient and efficient, it is accurate to differentiate, is suitble to the discriminating of green molasses and commodity honeybee kind source, is also applied for the identification in bee honey in the incorporation of certain proportion Apis mellifera honey.
Description
Technical field
The invention belongs to bee product field, a kind of method for relating generally to bee honey and Apis mellifera honey in discriminating, is to pass through
Bee honey and Apis mellifera honey during the method for Instrumental Analysis differentiates.
Technical background
Honey is the nectar, secretion or honeydew of honeybee herborization, after being combined with itself secretion, by honeycomb
Conversion, dehydration are stored to natural sweet substance made of ripe process.The secretion of honeybee is mainly some albumen in honey
Matter, including some digestive ferments and major royal jelly proteins etc..
Apis mellifera (Apis mellifera ligustica, abbreviation Apis mellifera) is a subspecies for apis mellifera, former
Italy is originated in, honey production ability is strong, and the ability for producing royal jelly is also strong, is the ideal kind of honey slurry and production, and is also pollen
The ideal kind of production, it may also be used for propolis produces.It is the bee species cultivated extensively in world wide, and Chinese state at present
The principal item of interior bee raising.
Apis cerana (Apis cerana cerana, abbreviation in bee) is a subspecies for Eastern bee, is that China is exclusive
Bee species, have using fragmentary nectariferous plant, acquisition power is strong, utilization rate is higher, honey harvest is long and adaptability, the disease-resistant energy of anti-mite
Power is strong, and consumption feed waits the incomparable advantage of apis mellifera Linnaeus less, is very suitable for Chinese Mountain Areas fixed point raising, but its honey output
It is relatively low, do not produce propolis and royal jelly.
Middle bee honey is also referred to as " scoliid honey ", and nutrition and health care effect is trusted by consumer deeply, however because yield is relatively low,
The market price is several times of common Apis mellifera honey.To earn in bee honey during some illegal retailers are pretended to be or are mixed with Apis mellifera honey
Juice is taken, causes greater loss to Chinese honeybee apiculture person and related bee product enterprise, while having caused the letter of consumer
Appoint crisis, the brand image of centering bee honey to adversely affect, is unfavorable for the sound development of bee's product market.
Invention content
The object of the present invention is to provide a kind of methods of bee honey and Apis mellifera honey in discriminating, are achieved by the steps of：
(1) alkane component extracts in honey：Honey 20g is weighed, is put in 50mL centrifuge tubes, 20mL pure water is added, is vortexed
15000g room temperature centrifuges 10min after shaking mixing, discards supernatant liquid, precipitation plus 60-90 DEG C of 10mL of petroleum ether, and be vortexed concussion 10-
15min, 8000g centrifuges 10min under normal temperature condition, and Aspirate supernatant obtains honey extract liquor H1 in 15mL centrifuge tubes.
(2) concentration of honey extract liquor：Honey extract liquor H1 is concentrated with nitrogen evaporator, nitrogen evaporator heating and temperature control
At 30-40 DEG C, extract liquor is concentrated into 1-2mL, stops nitrogen and blows, obtain extract liquor H2.
(3) preparation (1mg/mL) of positive melissane hydrocarbon standard working solution：The positive melissane standard items of 50mg are weighed in 50mL to hold
Measuring bottle, with 60-90 DEG C of dissolving of petroleum ether and constant volume, -20 DEG C of refrigerators preserve.
(4) gas chromatography-mass spectrum detects：Extract liquor H2 via hole diameters be 0.22um organic phase filter membrane filtering after take 1mL in
It is detected in sample bottle.
GC conditions：
Chromatographic column：InertCap 5,0.25mm × 30m × 0.25um, or quite；
Injector temperature：300℃；
Temperature program：Initial furnace temperature is 120 DEG C, continues 1min；180 DEG C are warming up to the speed of 30 DEG C/min, is continued
1min；300 DEG C are warming up to the speed of 10 DEG C/min, continues 15min；
Sampling volume：1 μ L, split sampling；
Carrier gas：High Purity Nitrogen (>=99.999%), flow velocity 1mL/min；
Mass Spectrometry Conditions：
Transfer tube temperature：300℃；
Ion source temperature：270℃；
Tetrode temperature：160℃；
Scan pattern：Full scan；
Scanning range：50-550nm.
(5) feature alkane component peak identifies：Apis mellifera honey has characteristic peak, middle bee honey to have spy in 29.2min in 21.3min
Peak is levied, 21.3min characteristic peaks are C31 alkane, and 29.2min characteristic peaks are C37 alkane, are formed according to two kinds of honey alkane components
Difference reaches discriminating purpose.
It is a further object to provide the discrimination methods in the discriminating of middle bee honey and Apis mellifera honey
Using.
A kind of method that the present invention proposes bee honey and Apis mellifera honey in discriminating, according to middle bee honey and Apis mellifera honey alkane
Hydrocarbon achievees the purpose that discriminating, C37 alkane are middle bee honey endemic element, C31 alkane is that Apis mellifera honey is special at the difference being grouped as
There is ingredient.The present invention reaches discriminating purpose by distinguishing the specific alkane component of different honeybee kind honey, can accurately differentiate
Middle bee honey and Apis mellifera honey.
The present invention is the difference of bee honey and Apis mellifera honey on alkane component composition in discovery by instrument analytical method
It is different, differentiate the honey of two kinds of source difference honeybee kinds with this, bee honey and Apis mellifera honey in can accurately differentiating solve market
In upper presence bee honey and Apis mellifera honey be difficult to differentiate and identify and Apis mellifera honey pretend to be and mix in bee honey this ask
Topic, contains pretend to be this confusion of bee honey in high price with low price Apis mellifera honey to a certain extent.Otherness in the method for the present invention
Alkane component is stabilized in middle bee honey and Apis mellifera honey, is not influenced by concentration processing and shelf life.The method of the present invention
Reasonable design, convenient and efficient, it is accurate to differentiate, is suitble to the discriminating of green molasses and commodity honeybee kind source, is also applied for certain proportion meaning
Identification in the incorporation of bee honey in bee honey.
Description of the drawings
Fig. 1 is Apis mellifera honey and middle bee honey gas chromatogram, is followed successively by M1, M2, M3, C1, C2, C3 from top to bottom.
Fig. 2 is blending honey gas chromatogram, is from top to bottom positive melissane standard items, blending sample 1, blending sample 2.
Specific implementation mode
The present invention is further described in conjunction with the accompanying drawings and embodiments.
Embodiment 1
The method of bee honey and Apis mellifera honey, is achieved by the steps of in a kind of discriminating：
(1) alkane component extracts in honey：Honey 20g is weighed, is put in 50mL centrifuge tubes, 20mL pure water is added, is vortexed
15000g room temperature centrifuges 10min after shaking mixing, discards supernatant liquid, precipitation plus 60-90 DEG C of 10mL of petroleum ether, and be vortexed concussion 10-
15min, 8000g centrifuges 10min under normal temperature condition, and Aspirate supernatant obtains honey extract liquor H1 in 15mL centrifuge tubes.
(2) concentration of honey extract liquor：Honey extract liquor H1 is concentrated with nitrogen evaporator, nitrogen evaporator heating and temperature control
At 30-40 DEG C, extract liquor is concentrated into 1-2mL, stops nitrogen and blows, obtain extract liquor H2.
(3) preparation (1mg/mL) of positive melissane hydrocarbon standard working solution：The positive melissane standard items of 50mg are weighed in 50mL to hold
Measuring bottle, with 60-90 DEG C of dissolving of petroleum ether and constant volume, -20 DEG C of refrigerators preserve.
(4) gas chromatography-mass spectrum detects：Extract liquor H2 via hole diameters be 0.22um organic phase filter membrane filtering after take 1mL in
It is detected in sample bottle.
GC conditions：
Chromatographic column：InertCap 5,0.25mm × 30m × 0.25um, or quite；
Injector temperature：300℃；
Temperature program：Initial furnace temperature is 120 DEG C, continues 1min；180 DEG C are warming up to the speed of 30 DEG C/min, is continued
1min；300 DEG C are warming up to the speed of 10 DEG C/min, continues 15min；
Sampling volume：1ul, split sampling；
Carrier gas：High Purity Nitrogen (>=99.999%), flow velocity 1mL/min.
Mass Spectrometry Conditions：
Transfer tube temperature：300℃；
Ion source temperature：270℃；
Tetrode temperature：160℃；
Scan pattern：Full scan；
Scanning range：50-550nm.
(5) feature alkane component peak identifies：Apis mellifera honey has characteristic peak, middle bee honey to have spy in 29.2min in 21.3min
Peak is levied, 21.3min characteristic peaks are C31 alkane, and 29.2min characteristic peaks are C37 alkane, are formed according to two kinds of honey alkane components
Difference reaches discriminating purpose.
Embodiment 2
To ensure that experiment effect, several cooperation bee farms and Zhejiang of the green molasses from Jinhua, Zhejiang Province city in the present embodiment are big
Experiment bee farm is learned, sample message see the table below 1.
1 honey sample information table of table
It is tested according to the method and step of embodiment one, gas chromatograph results are shown in Fig. 1, this it appears that two kinds in figure
The peak figure of honey has significant difference, Apis mellifera honey to have characteristic peak, middle bee honey to have characteristic peak in 29.2min in 21.3min, subsequently
Mass Spectrometric Identification result show that Apis mellifera honey in the characteristic peak ingredient of 21.3min is respectively C31 alkane components, middle bee honey exists
The characteristic peak ingredient of 29.2min is C37 alkane components.It can differentiate two kinds of different honeybee kinds sources according to the two otherness characteristic peak
Honey.
Embodiment 3
Several cooperation bee farms and Zhejiang University of the honey sample from Jinhua, Zhejiang Province city in the present embodiment test bee farm,
Two middle bee honey samples and two Apis mellifera honey samples are taken to be admixed, two kinds of honey blending ratios are 50%, admix honey
Sample number is 2.It is tested according to the method and step of embodiment 1, blending honey gas chromatograph results are shown in Fig. 2.It can from Fig. 2
There are two characteristic peaks of C31, C37 alkane will become apparent from blending honey, that is, proves that this honey sample is blending honey.
Although above-described embodiment has made detailed description to the present invention, protection scope of the present invention is not limited to
This can also make the solution of the present invention its modification without departing from center, they belong to protection scope of the present invention.
Claims (4)
1. a kind of method of bee honey and Apis mellifera honey in discriminating, which is characterized in that realized by following steps：
(1) alkane component extracts in honey：Honey 20g is weighed, is put in 50mL centrifuge tubes, 20mL pure water is added, be vortexed concussion
15000g room temperature centrifuges 10min after mixing, discards supernatant liquid, precipitation plus 60-90 DEG C of 10mL of petroleum ether, and be vortexed 10-15 points of concussion
Clock, 8000g centrifuges 10min under normal temperature condition, and Aspirate supernatant obtains honey extract liquor H1 in 15mL centrifuge tubes；
(2) concentration of honey extract liquor：Honey extract liquor H1 is concentrated with nitrogen evaporator, nitrogen evaporator heating and temperature control exists
30-40 DEG C, extract liquor is concentrated into 1-2mL, stops nitrogen and blows, obtain extract liquor H2；
(3) preparation (1mg/mL) of positive melissane hydrocarbon standard working solution：The positive melissane standard items of 50mg are weighed in 50mL volumetric flasks,
With 60-90 DEG C of dissolving of petroleum ether and constant volume, -20 DEG C of refrigerators preserve；
(4) gas chromatography-mass spectrum detects：Take 1mL in sample after the organic phase filter membrane filtering that extract liquor H2 via hole diameters are 0.22 μm
It is detected in bottle；
(5) feature alkane component peak identifies：Apis mellifera honey has characteristic peak, middle bee honey to have feature in 29.2min in 21.3min
Peak, 21.3min characteristic peaks are C31 alkane components, and 29.2min characteristic peaks are C37 alkane components, according to two kinds of honey alkane components
The difference of composition reaches discriminating purpose.
2. the method for bee honey and Apis mellifera honey in a kind of discriminating according to claim 1, which is characterized in that gas-chromatography
Condition：
Chromatographic column：InertCap 5,0.25mm × 30m × 0.25 μm, or quite；
Injector temperature：300℃；
Temperature program：Initial furnace temperature is 120 DEG C, continues 1min；180 DEG C are warming up to the speed of 30 DEG C/min, continues 1min；With
The speed of 10 DEG C/min is warming up to 300 DEG C, continues 15min；
Sampling volume：1 μ L, split sampling；
Carrier gas：>=99.999% High Purity Nitrogen, flow velocity 1mL/min；
Mass Spectrometry Conditions：
Transfer tube temperature：300℃；
Ion source temperature：270℃；
Tetrode temperature：160℃；
Scan pattern：Full scan；
Scanning range：50-550nm.
3. the method for bee honey and Apis mellifera honey in a kind of discriminating according to claim 1, which is characterized in that C37 alkane
For middle bee honey endemic element, C31 alkane is Apis mellifera honey endemic element, step (5) according to two kinds of honey alkane component differences,
Achieve the purpose that differentiate the two.
4. the method for bee honey and Apis mellifera honey is in middle bee honey and Apis mellifera honey in a kind of discriminating according to claim 1
Application in discriminating.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810008004.2A CN108362784A (en) | 2018-01-04 | 2018-01-04 | A kind of method of bee honey and Apis mellifera honey in discriminating |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810008004.2A CN108362784A (en) | 2018-01-04 | 2018-01-04 | A kind of method of bee honey and Apis mellifera honey in discriminating |
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| Publication Number | Publication Date |
|---|---|
| CN108362784A true CN108362784A (en) | 2018-08-03 |
Family
ID=63010771
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109470785A (en) * | 2018-10-23 | 2019-03-15 | 广东省生物资源应用研究所 | The construction method and identification application of a kind of natural middle bee honey of lungan flowers and natural Apis mellifera honey of lungan flowers finger-print |
| CN109470779A (en) * | 2018-09-28 | 2019-03-15 | 广东省生物资源应用研究所 | The construction method and identification application of a kind of natural middle bee honey of lychee flowers and natural Apis mellifera honey of lychee flowers finger-print |
| CN110376384A (en) * | 2019-06-24 | 2019-10-25 | 浙江大学 | The ELISA detection kit of bee honey and Apis mellifera honey in detection |
| CN111398497A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of kynurenine in identifying Chinese bee honey and apis mellifera honey |
| CN111398499A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of 3-amino-2-naphthoic acid in identifying apis cerana honey and apis mellifera honey |
| CN111398498A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of indole-3-methyl acetate in identifying apis cerana honey and apis mellifera honey |
| CN112595784A (en) * | 2020-10-19 | 2021-04-02 | 秦皇岛海关技术中心 | Method for distinguishing Chinese bee honey and Italian bee honey |
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2018
- 2018-01-04 CN CN201810008004.2A patent/CN108362784A/en active Pending
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| CN109470779B (en) * | 2018-09-28 | 2021-11-23 | 广东省科学院动物研究所 | Construction method and identification application of natural apis cerana litchi honey and natural apis mellifera litchi honey fingerprint |
| CN109470779A (en) * | 2018-09-28 | 2019-03-15 | 广东省生物资源应用研究所 | The construction method and identification application of a kind of natural middle bee honey of lychee flowers and natural Apis mellifera honey of lychee flowers finger-print |
| CN109470785B (en) * | 2018-10-23 | 2021-11-23 | 广东省科学院动物研究所 | Construction method and identification application of natural Chinese honeybee longan honey and natural Italian honeybee longan honey finger prints |
| CN109470785A (en) * | 2018-10-23 | 2019-03-15 | 广东省生物资源应用研究所 | The construction method and identification application of a kind of natural middle bee honey of lungan flowers and natural Apis mellifera honey of lungan flowers finger-print |
| CN110376384B (en) * | 2019-06-24 | 2020-06-16 | 浙江大学 | ELISA detection kit for detecting Chinese bee honey and Italian bee honey |
| CN110376384A (en) * | 2019-06-24 | 2019-10-25 | 浙江大学 | The ELISA detection kit of bee honey and Apis mellifera honey in detection |
| CN111398499A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of 3-amino-2-naphthoic acid in identifying apis cerana honey and apis mellifera honey |
| CN111398498A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of indole-3-methyl acetate in identifying apis cerana honey and apis mellifera honey |
| CN111398497A (en) * | 2020-03-19 | 2020-07-10 | 中国农业科学院蜜蜂研究所 | Application of kynurenine in identifying Chinese bee honey and apis mellifera honey |
| CN111398499B (en) * | 2020-03-19 | 2022-08-19 | 中国农业科学院蜜蜂研究所 | Application of 3-amino-2-naphthoic acid in identifying apis cerana honey and apis mellifera honey |
| CN111398497B (en) * | 2020-03-19 | 2022-08-19 | 中国农业科学院蜜蜂研究所 | Application of kynurenine in identifying Chinese bee honey and apis mellifera honey |
| CN111398498B (en) * | 2020-03-19 | 2023-01-03 | 中国农业科学院蜜蜂研究所 | Application of indole-3-methyl acetate in identifying apis cerana honey and apis mellifera honey |
| CN112595784A (en) * | 2020-10-19 | 2021-04-02 | 秦皇岛海关技术中心 | Method for distinguishing Chinese bee honey and Italian bee honey |
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